Sheep waste compound bacteria fermented organic fertilizer

Abstract

The invention relates to the field of biology, in particular to a sheep waste compound bacterium fermented organic fertilizer which is obtained by fermenting compound bacteria, and the compound bacteria comprise any one of bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae and clostridium butyricum. The organic fertilizer has the following beneficial effects: (1) the organic fertilizer is fermented by using four microorganisms at the same time, so that the fermentation is more sufficient, and the content of free amino acid is relatively high; (2) the organic fertilizer contains four kinds of probiotics, can obviously inhibit diseases and insect pests, and actual planting of tomatoes and Chinese cabbages also proves this; and (3) the problems that the sheep slaughtering wastes pollute the environment and are discharged are solved, a high-quality organic fertilizer source can be provided for agricultural production, and the wastes are comprehensively recycled.

Description

Technical Field

[0001] The invention relates to the biological field, and in particular to an organic fertilizer fermented from sheep waste using composite bacteria. Background Art

[0002] With the continuous development of my country's economy, people's consumption level continues to improve, consumption concepts continue to upgrade, and the demand for mutton is gradually increasing. In 2021, my country's mutton production will be 5.14 million tons and the demand will be 5.5487 million tons. In recent years, sheep farming in my country has shifted from extensive to intensive, and the scale of farming has continued to increase. In 2021, my country's sheep output will be 330 million heads and the number of sheep on hand will be 320 million heads, which also means a large amount of slaughter waste. The waste after slaughtering live sheep in the slaughterhouse is mainly the contents of the sheep's rumen, as well as a small amount of blood, feces, fur and some mutton scraps. These substances contain a small amount of nitrogen, phosphorus, potassium and a large amount of organic matter, which are essential nutrients for crops. However, there are many pathogens of zoonotic infectious diseases and parasitic diseases in this waste.

[0003] The current disposal methods are mainly to pile up waste in the factory area or transport it to landfill, which seriously pollutes the internal and surrounding environment of the enterprise, and the slaughterhouse has almost become a polluting factory. Therefore, it is necessary to develop new technologies for harmless waste treatment and resource utilization, prevent and eliminate pollution from slaughterhouse waste, which is very important for protecting the ecological environment and promoting sustainable agricultural development.

[0004] Composting is the fundamental way to make organic solid waste harmless and recyclable. Therefore, the composting research of organic solid waste has always been one of the hot topics in the field of environmental science. At present, there are many studies on the composting of organic solid waste such as livestock and poultry manure, straw, and sludge. However, there are few studies on the composting of slaughterhouse waste.

[0005] For example, the prior art CN 110078576 B discloses a method for producing improved saline-alkali land solid and liquid bio-organic fertilizers from slaughterhouse and breeding waste sewage and dead livestock and poultry straw, biogas residue and liquid biogas. The waste sewage and dead livestock and poultry from slaughterhouses and breeding farms are sterilized and disinfected, then compounded with whey powder, brown sugar and cornmeal, and then enzymolyzed with keratinase, papain, alkaline protease and lipase, inoculated with lactobacillus and yeast for fermentation, and then inoculated with licheniformis and gelatinous bacillus for fermentation. After the fermentation is completed, the supernatant obtained by filtering is the liquid bio-organic fertilizer, and the sediment is compounded with crushed straw and feces or biogas residue for composting and fermentation, and then compounded with straw carbon powder to produce solid bio-organic fertilizer.

[0006] Prior art CN 117567214A discloses a composite organic fertilizer for soil improvement and restoration and a preparation method thereof, wherein the composite organic fertilizer comprises a poultry and livestock excrement composition; plant straw powder; Chinese medicine waste residue; and fermented composite bacterial solution. The Chinese medicine active extract used acts on the seed solution of Bacillus licheniformis, Bacillus subtilis, Trichoderma longifolia, and Streptomyces tenuifolius in advance, and the Chinese medicine active extract has the effect of stimulating Bacillus licheniformis, Bacillus subtilis, Trichoderma longifolia, and Streptomyces tenuifolius, significantly improving the fermentation effect of Bacillus licheniformis, Bacillus subtilis, Trichoderma longifolia, and Streptomyces tenuifolius, and can significantly reduce the content of antibiotics in the organic fertilizer after fermentation, thereby reducing the secondary pollution of poultry and livestock excrement to the environment.

[0007] Prior art CN 104261913 B discloses a process for preparing pig manure bio-organic fertilizer by microbial fermentation. The product is made of pig manure as raw material, supplemented with straw, earthworm manure, seaweed, etc., through a two-step fermentation process of a biological fermentation agent. The product has the effects of both bio-organic fertilizer and seaweed fertilizer, and is rich in seaweed functional active ingredients and a large number of probiotics.

[0008] Prior art CN 116120113 B discloses a method for preparing biological organic fertilizer using livestock and poultry waste, which comprises the following steps: dissolving Bacillus subtilis, Bacillus cereus, Clostridium butyricum and nitrogen-fixing bacteria in water and adding agar to obtain a mixed bacterial solution, adding attapulgite powder to the mixed bacterial solution and freeze-drying to obtain a composite bacterial agent A; stirring and mixing livestock and poultry manure and livestock and poultry wastewater evenly, then adding the composite bacterial agent A to mix and ferment to obtain slurry A, adding a flocculant to the slurry A for reaction and filtration to obtain slurry B; mixing sweet potato stems and leaves, bagasse, soybean meal and citronella, crushing them, mixing them with water, adding the composite bacterial agent B to ferment to obtain slurry C; the composite bacterial agent B comprises cellulase, hemicellulase and yeast; mixing slurry B and slurry C and continuing to ferment to obtain liquid organic fertilizer.

[0009] Due to the different livestock species and feed formulas, the waste left after slaughtering different livestock is also different, and the physical and chemical properties and other characteristics of the high-temperature fermentation process are also different. Therefore, it is urgent to explore a method suitable for the compound bacterial fermentation of sheep waste, and to prepare the corresponding organic fertilizer, so as to solve the problem of sheep slaughter waste polluting the environment and its outlet, and provide a high-quality organic fertilizer source for agricultural production, and realize the comprehensive utilization of waste resources. Summary of the invention

[0010] In order to solve the above problems, the present invention first provides a sheep waste composite bacteria fermented organic fertilizer, the organic fertilizer is obtained by composite bacteria fermentation, and the composite bacteria includes any one of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae, and Clostridium butyricum.

[0011] Preferably, the composite bacteria comprises any two of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae, and Clostridium butyricum.

[0012] Preferably, the composite bacteria comprises any three of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae, and Clostridium butyricum.

[0013] Preferably, the composite bacteria consists of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae and Clostridium butyricum.

[0014] The present invention also provides a method for preparing organic fertilizer fermented from sheep waste by composite bacteria, comprising the following steps:

[0015] (1) Compound bacteria propagation; (2) Compound bacteria fermentation.

[0016] Preferably, the composite bacteria ferments into natural compost.

[0017] Preferably, the natural composting is turned over once every 2 days.

[0018] The present invention finally provides the application of the sheep waste composite bacteria fermented organic fertilizer, and the application is crop planting.

[0019] Preferably, the crops include vegetables or forage grass.

[0020] Preferably, the vegetables include tomatoes and cabbage.

[0021] Compared with the prior art, the present invention has at least the following beneficial effects:

[0022] (1) The organic fertilizer fermentation of the present invention uses four kinds of microorganisms to ferment simultaneously, and the fermentation is more complete and the free amino acid content is higher;

[0023] (2) The organic fertilizer of the present invention contains four kinds of probiotics, which can significantly inhibit pests and diseases, and the actual planting of tomatoes and cabbages also proves this point;

[0024] (3) The present invention solves the problem of environmental pollution caused by sheep slaughtering waste and its disposal, and can provide a high-quality organic fertilizer source for agricultural production, thereby realizing the comprehensive utilization of waste resources. DETAILED DESCRIPTION

[0025] In order to make the technical problems, technical solutions and advantages to be solved by the present invention more clear, specific embodiments are described in detail below.

[0026] Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae, and Clostridium butyricum were purchased from China Agricultural Culture Collection Center (ACCC).

[0027] Example 1

[0028] Sheep waste was provided by Inner Mongolia Green Grass Biotechnology Co., Ltd. and its basic characteristics are shown in Table 1.

[0029] Table 1 Basic characteristics of sheep waste

[0030]

[0031] Example 2 Preparation of organic fertilizer.

[0032] The preparation method of the organic fertilizer comprises the following steps:

[0033] (1) Propagation of composite bacteria: Weigh 10 kg bran (high temperature sterilized), 0.8 kg brown sugar, 200 g composite bacteria and 1.5 L warm water, mix well, put into special bags for cultivating bacteria, put into an incubator, and culture at 30°C for 80 hours to obtain propagation composite bacteria. Then weigh 6 kg composite bacteria and 10 kg bran, mix well, and then mix with 500 kg sheep waste and pile for fermentation.

[0034] The composite bacteria include Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae and Clostridium butyricum in a ratio of 1:1:1:1.

[0035] (2) Composting: The mixed mixture is composted and fermented naturally for 16 days, with regular stirring; the pile is turned every 2 days. Fermentation is considered complete when the pile temperature drops from high to low and stops changing below 40°C.

[0036] A comparative example was set according to Table 2, except that the types of composite bacteria were different, the ratios were the same, and the other conditions were the same as those in Example 2;

[0037] Table 2 Differences between Comparative Example and Example 2 (- means no)

[0038]

[0039]

[0040] Blank control: 500 kg of sheep waste and 10 kg of bran were fully mixed, and no composite bacteria were added. The compost was naturally fermented for 16 days, and stirred regularly. The compost was turned every 2 days. Fermentation was considered complete when the temperature of the compost dropped from high to low and did not change below 40°C.

[0041] Example 3 Determination of organic fertilizer index

[0042] The fertilizer indexes of the organic base fertilizer prepared above were tested, and the results are shown in Table 3 below.

[0043] Table 3 Organic fertilizer indicators

[0044]

[0045]

[0046] The results are shown in Table 3: the organic fertilizer of Example 2 has the lowest organic matter content and the highest free amino acid content, indicating that the fermentation is more complete and the effect is most significant (p<0.05).

[0047] Experimental Example 4: Tomato Planting Test:

[0048] 17 mu of farmland were selected for field experiments and divided into 17 groups on average, each group with one mu; tomato planting was carried out using the organic fertilizer prepared by the method described in Example 2, Comparative Examples 1-15, and the control group, and the relative yield increase rate and relative incidence rate of diseases and insect pests of tomatoes were calculated based on the control group at the same application rate. The specific results are shown in Table 4.

[0049] Table 4 Tomato planting results

[0050]

[0051]

[0052] The results are shown in Table 4: The organic fertilizer of Example 2 has the best tomato planting yield increase rate and the lowest incidence of pests and diseases, and the effect is most significant (p<0.05), indicating that the combined fermentation of the four bacteria is more complete, the content of free amino acids is higher, and it contains a variety of microorganisms, which inhibits some pathogenic microorganisms or insect eggs, resulting in a lower incidence of pests and diseases.

[0053] Example 5: Cabbage Planting Test

[0054] 17 mu of farmland was selected for field experiments and divided into 17 groups on average, each group with one mu; tomato planting was carried out using the organic fertilizer prepared by the method described in Example 2, Comparative Examples 1-15, and the control group, and the relative yield increase rate of cabbage was calculated based on the control group at the same application amount (50 kg / mu / month). The specific results are shown in Table 4.

[0055] Table 4 Cabbage planting results

[0056]

[0057]

[0058] The results are shown in Table 5: the organic fertilizer of Example 2 has the best cabbage planting yield increase rate and the lowest incidence of pests and diseases, and the effect is the most significant (p<0.05), indicating that the combined fermentation of the four bacteria is more complete, the content of free amino acids is higher, and it contains a variety of microorganisms, which inhibits some pathogenic microorganisms or insect eggs, resulting in a lower incidence of pests and diseases.

[0059] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A sheep waste compound bacteria fermented organic fertilizer, characterized in that: The organic fertilizer is obtained by fermentation with composite bacteria, and the composite bacteria comprises any one of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae, and Clostridium butyricum.

2. The organic fertilizer according to claim 1, wherein The composite bacteria comprises any two of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae and Clostridium butyricum.

3. The organic fertilizer according to claim 1, wherein The composite bacteria comprises any three of Bacillus subtilis, lactic acid bacteria, Saccharomyces cerevisiae and Clostridium butyricum.

4. The organic fertilizer according to claim 3, wherein The composite bacteria consists of bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae and clostridium butyricum.

5. A method for preparing organic fertilizer fermented from sheep waste by composite bacteria, characterized in that: The method comprises the following steps: (1) compound bacteria propagation; and (2) compound bacteria fermentation.

6. The preparation method according to claim 5, characterized in that: The composite bacteria fermentation is natural compost.

7. The preparation method according to claim 6, characterized in that: The natural composting is turned over once every 2 days.

8. The use of the sheep waste composite bacteria fermentation organic fertilizer as claimed in any one of claims 1 to 4, characterized in that: The application is crop cultivation.

9. The use according to claim 8, characterized in that The crops include vegetables or forage.

10. The use according to claim 9, characterized in that The vegetables include tomatoes and cabbage.

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