Hydrogen sulfide sensing detector based on cholesterol modified lipid perovskite as well as preparation method and application of hydrogen sulfide sensing detector

Abstract

The invention discloses a hydrogen sulfide sensing detector based on cholesterol modified lipid perovskite as well as a preparation method and application of the hydrogen sulfide sensing detector, and belongs to the technical field of biological industry. According to the invention, phospholipid and cholesterol with high biocompatibility are adopted to construct a stable protective layer for the hydrophobic perovskite nanoparticles, so that the hydrophobic perovskite nanoparticles can emit light stably, the stability of perovskite is remarkably improved, and an ideal platform is provided for accurate detection of hydrogen sulfide. According to the hydrogen sulfide sensing detector based on cholesterol modified lipid perovskite, the detection limit and response speed of hydrogen sulfide are remarkably improved, the risk of false negative results is reduced, a visual and visual detection means is provided for detection of hydrogen sulfide, the detection limit is as low as ng / mL, development of a gas sensing technology is promoted, and the application prospect is wide. The accuracy and efficiency of harmful gas detection are improved, and meanwhile, a new research direction is provided for application of nano materials in the field of environmental monitoring.

Description

Technical Field

[0001] The present invention belongs to the technical field of bio-industry, and in particular relates to a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite and a preparation method and application thereof. Background Art

[0002] Hydrogen sulfide (H2S) is a highly toxic gas with a distinct rotten egg smell, known for its potential to block cellular respiration by binding to iron in mitochondrial cytochrome enzymes. Hydrogen sulfide is widely present in many industrial production processes, especially in petrochemicals, coal mining, and sewage treatment. This gas not only poses a serious threat to human health, but is also a major hidden danger to the ecological environment. Continuous exposure to H2S environment can cause coma, severe poisoning, rapid respiratory arrest, inactivation of the olfactory system (30-40ppm H2S), and even death (greater than 250ppmH2S). Based on the widespread poisoning effects of H2S (i.e., attacking the nervous system and several other systems in the body), the Occupational Safety and Health Administration (OSHA) has set the exposure limit at 10ppm (8 hours). In addition to the harm to human health, hydrogen sulfide also causes serious damage to metal equipment due to its strong corrosiveness. In industrial production processes, hydrogen sulfide can react with metal surfaces, causing corrosion and damage to equipment, which not only increases maintenance costs, but more importantly, may cause safety accidents and threaten the life safety of operators. The corrosive effect of hydrogen sulfide is not limited to metals. It can also damage building materials, pipelines, and electronic equipment, thus affecting the stability and reliability of industrial production. In view of these hazards of hydrogen sulfide, it is particularly important to effectively detect and control it. In industrial environments, rapid and accurate monitoring of hydrogen sulfide concentration is an important measure to prevent poisoning accidents, ensure personnel safety, and protect the environment. However, the detection of hydrogen sulfide faces many challenges, such as its concentration fluctuations in the air, cross-interference with other gases, and the performance limitations of the detection equipment itself.

[0003] Therefore, there is an urgent need for a hydrogen sulfide detector with good stability, fast response speed and low detection limit to solve the above problems. Summary of the invention

[0004] In order to solve the above technical problems, the present invention proposes a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite and a preparation method and application thereof.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite, comprising the following steps:

[0007] (1) mixing a CsPbBr3 nanocrystal solution with an organic solvent containing cholesterol and phospholipids, and then performing rotary evaporation to obtain a green film;

[0008] (2) The green film obtained in step (1) is mixed with water for hydration, and then subjected to ultrasound and centrifugation to obtain the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite.

[0009] Preferably, in step (1), the mass ratio of CsPbBr3 nanocrystals to cholesterol and phospholipids in the CsPbBr3 nanocrystal solution is (4-8):(5-18):(55-65).

[0010] Preferably, in step (1), the phospholipid comprises soybean lecithin.

[0011] Preferably, in step (1), the rotary evaporation temperature is 32-37° C. and the time is 10 min.

[0012] Preferably, in step (1), the method for preparing CsPbBr3 nanocrystals in the CsPbBr3 nanocrystal solution comprises: mixing PbBr2, CsBr, oleylamine and oleic acid in N,N-dimethylformamide, and obtaining a precursor solution by stirring; and quickly adding the precursor solution into ethyl acetate at 32-37°C, and obtaining the CsPbBr3 nanocrystals by stirring and centrifuging.

[0013] Preferably, in step (2), the ratio of the green film to water is 0.064 g:6 mL.

[0014] Preferably, in step (2), the ultrasonic time is 2-5 min and the power is 50 W.

[0015] Preferably, in step (2), the centrifugal speed is 12000 rpm and the time is 10 min.

[0016] The present invention provides a hydrogen sulfide sensing detector based on cholesterol-modified lipid perovskite prepared by the preparation method described in the above technical scheme.

[0017] The present invention also provides the use of the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite described in the above technical solution in detecting hydrogen sulfide.

[0018] Compared with the prior art, the present invention has the following advantages and technical effects:

[0019] The present invention uses highly biocompatible phospholipids and cholesterol to construct a stable protective layer for hydrophobic perovskite nanoparticles, so that it can stably emit light, which not only significantly improves the stability of perovskite, but also provides an ideal platform for the accurate detection of hydrogen sulfide. The hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite provided by the present invention significantly improves the detection limit and response speed of hydrogen sulfide, reduces the risk of false negative results, and provides an intuitive visual detection method for the detection of hydrogen sulfide. The detection limit is as low as ng / mL, which helps to promote the development of gas sensing technology, improve the accuracy and efficiency of harmful gas detection, and also provides a new research direction for the application of nanomaterials in the field of environmental monitoring. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0021] Figure 1 Schematic diagram of the process of preparing the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite in Example 1;

[0022] Figure 2 TEM image of CsPbBr3@PL prepared in Example 1;

[0023] Figure 3 TEM image and element mapping of CsPbBr3@PL prepared in Example 1;

[0024] Figure 4 Actual pictures of CsPbBr3 n-hexane solution (a) and CsPbBr3@LP aqueous solution (b) under fluorescent light irradiation;

[0025] Figure 5 The photoluminescence intensity diagram of CsPbBr3@PL prepared in Example 1 at different time points in PBS;

[0026] Figure 6 The fluorescence intensity diagram is a graph after different concentrations of sodium sulfide are incubated with the aqueous solution of CsPbBr3@PL prepared in Example 1 for 10 minutes;

[0027] Figure 7 This is a graph showing the detection results of the CsPbBr3@PL prepared in Example 1 on the hydrogen sulfide content in colorectal cancer cells. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] The embodiment of the present invention provides a method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite, comprising the following steps:

[0031] (1) mixing a CsPbBr3 nanocrystal solution with an organic solvent containing cholesterol and phospholipids, and then performing rotary evaporation to obtain a green film;

[0032] (2) The green film obtained in step (1) is mixed with water for hydration, and then subjected to ultrasound and centrifugation to obtain the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite.

[0033] In a preferred embodiment, in step (1), the mass ratio of CsPbBr3 nanocrystals to cholesterol and phospholipids in the CsPbBr3 nanocrystal solution is (4-8): (5-18): (55-65). The present invention constructs a stable protective layer for hydrophobic perovskite nanoparticles by using highly biocompatible phospholipids and cholesterol, so that it can stably emit light, which not only significantly improves the stability of perovskite, but also provides an ideal platform for the accurate detection of hydrogen sulfide. The present invention controls the mass ratio of CsPbBr3 nanocrystals to cholesterol and phospholipids within the above range, which is beneficial to improving the stability of perovskite. Changing the dosage of cholesterol and phospholipids will reduce the stability of CsPbBr3 nanocrystals.

[0034] In a preferred embodiment, in step (1), the phospholipid comprises soybean lecithin; more preferably, the phospholipid comprises soybean lecithin and DSPE-PEG.

[0035] In a preferred embodiment, in step (1), the organic solvent is dichloromethane.

[0036] In a preferred embodiment, in step (1), the preparation method of CsPbBr3 nanocrystals in the CsPbBr3 nanocrystal solution comprises: mixing PbBr2, CsBr, oleylamine and oleic acid in N,N-dimethylformamide, and stirring to obtain a precursor solution; and rapidly adding the precursor solution into ethyl acetate at 32-37°C, stirring and centrifuging to obtain the CsPbBr3 nanocrystals. The CsPbBr3 nanocrystals prepared by the method provided by the present invention have good dispersibility.

[0037] In a preferred embodiment, the usage ratio of PbBr2, CsBr, oleylamine, oleic acid and N,N-dimethylformamide is 0.367 g:0.212 g:0.1 mL:0.5 mL:5.0 mL.

[0038] In a preferred embodiment, the stirring temperature is room temperature and the stirring time is 5 minutes.

[0039] In a preferred embodiment, the volume ratio of the precursor solution to ethyl acetate is 0.5:10.

[0040] In a preferred embodiment, the stirring speed during the stirring and centrifugation is 1000 rpm and the time is 10 min; the centrifugation speed is 1000 rpm and the time is 5 min.

[0041] In a preferred embodiment, in step (1), the size of the CsPbBr3 nanocrystals in the CsPbBr3 nanocrystal solution is 10-20 nm.

[0042] In a preferred embodiment, in step (1), the solvent of the CsPbBr3 nanocrystal solution is n-hexane.

[0043] In a preferred embodiment, in step (1), the temperature of the rotary evaporation is 32-37°C, the time is 10 minutes, and the equipment is a rotary evaporator; the rotary evaporation is performed under vacuum. The present invention removes the solvent n-hexane and the organic solvent dichloromethane in the CsPbBr3 nanocrystal solution by rotary evaporation, and forms a green film at the same time. The present invention controls the temperature and time of the rotary evaporation within the above range, which is conducive to removing the organic solvent and preventing the oxidation of the CsPbBr3 nanocrystals. Changing the temperature and time of the rotary evaporation will affect the formation of the green film.

[0044] In a preferred embodiment, in step (2), the ratio of the amount of the green film to water is 0.064 g: 6 mL. In the present invention, the green film is mixed with water for hydration, and cholesterol and phospholipids can be freely assembled into a shell structure in the solution, thereby encapsulating the CsPbBr3 nanocrystals in the lipid layer, thereby improving the stability of the perovskite.

[0045] In a preferred embodiment, in step (2), the ultrasonication time is 2-5 min and the power is 50 W.

[0046] In a preferred embodiment, in step (2), the centrifugal speed is 12000 rpm and the time is 10 min. The present invention performs purification by centrifugation.

[0047] The present invention provides a hydrogen sulfide sensing detector based on cholesterol-modified lipid perovskite prepared by the preparation method described in the above technical scheme.

[0048] In a preferred embodiment, the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite has a particle size of 40-110 nm and a spherical shape.

[0049] The hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite provided by the present invention has long-term stability, reusability and applicability under complex environmental conditions, and also has excellent sensitivity and specificity.

[0050] The present invention also provides the use of the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite described in the above technical solution in detecting hydrogen sulfide.

[0051] The room temperature in the embodiments of the present invention refers to "25±2°C".

[0052] In the following examples, cesium bromide (CsBr, 99.5%), lead bromide (PbBr2, 99%), dichloromethane (DCM), oleic acid (OA, 90%), oleylamine (OAm, 80-90%) and N,N-dimethylformamide (DMF, 100%) were purchased from Aladdin Industries (Shanghai, China). Soy lecithin and cholesterol were purchased from Meilun Biotechnology (Dalian, China). Hexane was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Phosphate buffered saline (PBS, pH 7.4) was purchased from Nanjing Bookman Biotechnology Co., Ltd. DSPE-PEG was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0053] Example 1

[0054] A preparation method for a hydrogen sulfide sensing detector based on cholesterol-modified lipid perovskite:

[0055] (1) 0.367 g PbBr2, 0.212 g CsBr, 0.1 mL OAm and 0.5 mL OA were mixed in 5.0 mL DMF and stirred at room temperature for 5 min to obtain a precursor solution; 0.5 mL of the precursor solution was quickly added into 10 mL ethyl acetate at 37 °C, and then stirred at 1000 rpm for 10 min, and centrifuged at 10000 rpm for 5 min to obtain CsPbBr3 nanocrystals, denoted as CsPbBr3NCs;

[0056] (2) dispersing the CsPbBr3 nanocrystals obtained in step (1) in n-hexane to obtain a CsPbBr3 nanocrystal solution; mixing 500 μL of the CsPbBr3 nanocrystal solution with dichloromethane containing cholesterol, soybean lecithin and DSPE-PEG, wherein the mass ratio of the CsPbBr3 nanocrystals to cholesterol, soybean lecithin and DSPE-PEG in the CsPbBr3 nanocrystal solution is 4:5:45:10, and then using a rotary evaporator to rotary evaporate at 37° C. under vacuum conditions for 10 min to obtain a green film;

[0057] (3) The green film obtained in step (2) was mixed with deionized water at a ratio of 0.064 g:6 mL for hydration, ultrasonicated at 50 W for 2 min, and then centrifuged at 12000 rpm for 10 min and filtered. The resulting precipitate was a hydrogen sulfide sensor based on cholesterol-modified lipid perovskite, denoted as CsPbBr3@PL.

[0058] Figure 1 FIG. 1 is a schematic diagram of the process of preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite in Example 1. Figure 1 It can be seen that CsPbBr3 NCs are first synthesized with PbBr2, CsBr, oleylamine and oleic acid as raw materials and then dissolved in n-hexyl ring to obtain CsPbBr3 NCs solution. The CsPbBr3 NCs solution is then mixed with dichloromethane containing cholesterol and phospholipids. The organic solvent is then removed by thin film spinning method, and deionized water is injected. Cholesterol and phospholipids are freely assembled into shell structures in the solution, thereby encapsulating a large number of CsPbBr3 NCs.

[0059] The CsPbBr3@PL prepared in Example 1 was characterized by transmission electron microscopy (TEM). Figure 2 As shown. Figure 2It can be clearly seen that there are a large number of CsPbBr3NCs (small black dots in the figure) in CsPbBr3@PL, proving that CsPbBr3NCs are successfully encapsulated by phospholipids and cholesterol. It can also be seen that CsPbBr3NCs have good monodispersity in CsPbBr3@PL, with a size of about 10nm, and CsPbBr3@PL has a spherical structure of about 110nm. This shows that through the method of free encapsulation of phospholipids and cholesterol, CsPbBr3NCs are successfully encapsulated in the lipid layer to form larger composite nanoparticles.

[0060] Figure 3 TEM image and element mapping of CsPbBr3@PL prepared in Example 1. Figure 3 As shown in Figure 1, the elemental map shows a uniform distribution of Cs, Pb, Br, P, N, and O, which confirms the existence of CsPbBr3 perovskite nanoparticles and lipid layers. Among them, Cs, Pb, and Br correspond to the constituent elements of perovskite, P may come from the phospholipid molecules of the lipid layer, and N and O may come from the nitrogen and oxygen elements in the lipid layer. The uniform distribution of elements indicates that in CsPbBr3@PL, CsPbBr3 nanoparticles are uniformly encapsulated in the lipid layer, and this uniformity is crucial to ensure the stability and reproducibility of material properties.

[0061] 0.011 g of the CsPbBr3 nanocrystals in Example 1 were dispersed in 2 mL of n-hexane solution to obtain a CsPbBr3 n-hexane solution. 0.011 g of the CsPbBr3@PL prepared in Example 1 was dispersed in 2 mL of deionized water to obtain a CsPbBr3@PL aqueous solution. The CsPbBr3 n-hexane solution and the CsPbBr3@PL aqueous solution were irradiated under a fluorescent lamp. Figure 4 . Figure 4 The actual pictures of CsPbBr3 n-hexane solution (a) and CsPbBr3@LP aqueous solution (b) under fluorescent light. Figure 4 It can be seen that both the CsPbBr3 n-hexane solution and the CsPbBr3@LP aqueous solution are bright green, which further illustrates that the CsPbBr3 nanocrystals encapsulated in the lipid cholesterol layer show better stability, which may be because the lipid layer provides additional protection, preventing the nanoparticles from reacting with the aqueous solution and reducing the formation of surface defects. In biomedical applications, the stability of nanoparticles is crucial. Stable nanoparticles can ensure that they remain functional in the body, reduce potential toxicity and immune responses, and improve their application in diagnosis and treatment.

[0062] Figure 5 The photoluminescence intensity of CsPbBr3@PL prepared in Example 1 at different time points in PBS. Figure 5It can be seen that the photoluminescence spectrum of CsPbBr3@PL shows obvious luminescence characteristics, which indicates that CsPbBr3 nanocrystals have excellent optical properties; even after being co-encapsulated by phospholipids and cholesterol, the photoluminescence spectrum remains unchanged. This may be because the encapsulation of the lipid layer changes the local environment of the nanoparticles, affecting the band structure and luminescence center; as time increases, its photoluminescence spectrum still shows obvious luminescence characteristics, and the fluorescence intensity remains unchanged, which indicates that the co-encapsulation of CsPbBr3 nanocrystals by phospholipids and cholesterol does not significantly affect the luminescence properties of CsPbBr3 nanocrystals.

[0063] 100 μg / mL sodium sulfide standard solution (S 2- 10 μL of the sodium sulfide dilution was placed in different EP tubes, and then 100 μL of the aqueous solution of CsPbBr3@PL prepared in Example 1 with a concentration of 0.17 mg / mL (based on the content of CsPbBr3) was added. After gently shaking for 10 minutes, the fluorescence value of each well was immediately detected under 365 nm excitation light using a fluorescence microplate reader. The test results are shown in Figure 6 .

[0064] Figure 6 The fluorescence intensity diagram is shown after incubation of CsPbBr3@PL aqueous solution prepared in Example 1 with different concentrations of sodium sulfide for 10 minutes. Figure 6 It can be seen that as the concentration of sodium sulfide increases, the photoluminescence intensity of CsPbBr3@PL decreases and changes linearly, indicating that the CsPbBr3@PL prepared by the present invention can be used to detect the concentration of hydrogen sulfide.

[0065] Detection of hydrogen sulfide content in colorectal cancer cells by CsPbBr3@PL prepared in Example 1: HCT-116 cells were inoculated into a confocal culture dish. After 24 hours, HCT116 cells were treated with a cell culture solution with a concentration of 100 μM H2S for 2 hours, then rinsed twice with 1×PBS, and then suspended in 100 μL of 1×PBS containing 1% BSA. 100 μL of CsPbBr3@PL prepared in Example 1 was added. After incubation at room temperature for 20 minutes, 1 mL of 1×PBS solution was added. After washing the cells three times at room temperature, the cells were immediately detected by a high-resolution spectrofluorimeter. The results are shown in FIG. Figure 7 .

[0066] Figure 7The results of the detection of hydrogen sulfide content in colorectal cancer cells by CsPbBr3@PL prepared in Example 1 are shown in Figure A for HCT116 cells and Figure B for HCT116 cells pre-treated with a cell culture medium containing 100 μM H2S for 2 hours. Figure 7 It can be seen that the cells in Figure A show green fluorescence, while the fluorescence in HCT116 cells treated with high concentrations of hydrogen sulfide is quenched, indicating that CsPbBr3@PL can detect the hydrogen sulfide content in colorectal cancer cells.

[0067] Comparative Example 1

[0068] The difference from Example 1 is that in step (2), rotary evaporation is carried out under vacuum conditions at 27° C., and the other steps and process parameters are the same as in Example 1.

[0069] Comparative Example 2

[0070] The difference from Example 1 is that in step (2), rotary evaporation is carried out at 37° C. under vacuum conditions for 5 min, and the other steps and process parameters are the same as in Example 1.

[0071] Comparative Example 3

[0072] The difference from Example 1 is that in step (3), the green film obtained in step (2) is mixed with deionized water in a ratio of 0.084 g:6 mL for hydration, and the other steps and process parameters are the same as in Example 1.

[0073] Comparative Example 4

[0074] The difference from Example 1 is that in step (2), 500 μL of CsPbBr3 nanocrystal solution is mixed with dichloromethane containing soybean lecithin and DSPE-PEG, and the other steps and process parameters are the same as Example 1.

[0075] 10 μL of 100 μg / mL sodium sulfide standard solution (S 2- The samples were placed in different EP tubes, and then 100 μL of 0.17 mg / mL (based on the content of CsPbBr3) of the aqueous solution of CsPbBr3@PL prepared in Example 1 and Comparative Examples 1-4 was added. After gently shaking for 2 minutes, the fluorescence value of each well was immediately detected under an excitation light of 365 nm using a fluorescence microplate reader. The results are shown in Table 1.

[0076] Table 1 Performance of CsPbBr3@PL prepared in Example 1 and Comparative Examples 1-4

[0077] Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Fluorescence value 24226 35978 38189 38920 40029

[0078] It can be seen from Table 1 that the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite provided by the present invention has excellent sensitivity.

[0079] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite, characterized in that: The following steps are involved: (1) mixing a CsPbBr3 nanocrystal solution with an organic solvent containing cholesterol and phospholipids, and then performing rotary evaporation to obtain a green film; (2) The green film obtained in step (1) is mixed with water for hydration, and then subjected to ultrasound and centrifugation to obtain the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite.

2. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (1), the mass ratio of CsPbBr3 nanocrystals to cholesterol and phospholipids in the CsPbBr3 nanocrystal solution is (4-8):(5-18):(55-65).

3. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (1), the phospholipid comprises soybean lecithin.

4. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (1), the rotary evaporation temperature is 32-37° C. and the time is 10 min.

5. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (1), the preparation method of CsPbBr3 nanocrystals in the CsPbBr3 nanocrystal solution includes: mixing PbBr2, CsBr, oleylamine and oleic acid in N,N-dimethylformamide, and obtaining a precursor solution by stirring; and quickly adding the precursor solution into ethyl acetate at 32-37°C, and obtaining the CsPbBr3 nanocrystals by stirring and centrifuging.

6. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (2), the usage ratio of the green film to water is 0.064 g:6 mL.

7. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (2), the ultrasonic time is 2-5 minutes and the power is 50W.

8. The method for preparing a hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 1, characterized in that: In step (2), the centrifugal speed is 12000 rpm and the time is 10 min.

9. A hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the hydrogen sulfide sensor detector based on cholesterol-modified lipid perovskite according to claim 9 in detecting hydrogen sulfide.

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