Culture dish applied to bacterial drug sensitive test

Abstract

The invention belongs to the field of biological experiment instruments, and particularly relates to a culture dish applied to a bacterial drug sensitive test, which comprises a culture dish body, a supporting frame is arranged on the outer side of the culture dish body; supporting legs are symmetrically and fixedly connected to the side wall of the supporting frame. Threaded rods are symmetrically arranged on the side wall of the supporting frame. The threaded rod penetrates through the surface of the supporting frame and is in threaded connection with the supporting frame; through the arrangement of the through hole structure, the design is simple and convenient to operate, and when the culture dish body is used, the contact between the bottom of the culture dish and a contact surface can be reduced, so that the influence on heat transfer can be reduced, and the culture dish body is heated more uniformly; and the culture dish bodies with different sizes and models can be extruded and fixed, so that the phenomenon that the culture dish bodies shake can be reduced.

Description

Technical Field

[0001] The invention belongs to the field of biological experimental instruments, in particular to a culture dish used for bacterial drug sensitivity test. Background Art

[0002] With the development of society, culture dishes are often needed in other fields such as biology, medicine, food industry, environmental science, etc. A culture dish is a laboratory vessel used for culturing microorganisms or cells.

[0003] When conducting bacterial drug sensitivity tests, culture dishes are often required. The culture dishes are usually filled with culture media suitable for bacterial growth, which can provide bacteria with the necessary nutrients, such as carbon sources, nitrogen sources, inorganic salts, growth factors, etc., and the culture dishes are usually strictly disinfected before use to ensure that they are in a sterile state. In bacterial drug sensitivity tests, a sterile environment is crucial to ensure the accuracy of experimental results. In the prior art, it has been found through long-term observation and use that in the existing bacterial drug sensitivity test, the culture dish usually needs to be placed in a constant temperature incubator for culture. If the bottom of the culture dish has a large contact with the contact surface, the heat transfer may be affected, resulting in uneven heating in different areas of the bottom of the culture dish. For example, the heat transfer may be faster and the temperature may be higher in the parts with close contact, while the temperature may be lower in the parts with relatively loose contact. This uneven heating will affect the growth rate and growth state of the bacteria, and thus affect the results of the drug sensitivity test. To this end, the present invention provides a culture dish for use in bacterial drug sensitivity testing. Summary of the invention

[0004] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0005] The technical solution adopted by the present invention to solve its technical problems is: the present invention is a culture dish used for bacterial drug sensitivity test, comprising a culture dish body; a support frame is arranged on the outer side of the culture dish body; legs are symmetrically fixedly connected to the side walls of the support frame; threaded rods are symmetrically arranged on the side walls of the support frame; the threaded rods penetrate the surface of the support frame and are threadedly connected thereto; a handle is fixedly connected to the end of the threaded rod; a push plate is rotatably connected to the end of the threaded rod; a guide rod is symmetrically fixedly connected to the side wall of the push plate; the end of the guide rod is fixedly connected to the side wall of the support frame; multiple groups of spring rods are fixedly connected to the side wall of the push plate; a rubber block is fixedly connected to the end of the spring rod; the rubber block is arranged in an arc shape; multiple groups of support rods are arranged at the bottom end of the support frame; a support plate is arranged on the side wall of the support rod; multiple groups of through holes are opened on the side wall of the support plate; the through holes penetrate the interior of the support plate; and a cover is arranged on the top of the culture dish body.

[0006] Preferably, the side wall of the culture dish body is provided with a plurality of mounting grooves; the inner side of the mounting groove is slidably connected with a mounting block; the mounting block is connected to the mounting groove by a sliding connection; the side wall of the mounting block is fixedly connected with a placement box; and the side wall of the culture dish body is fixedly connected with a plurality of marking columns.

[0007] Preferably, the side walls of the support frame are symmetrically fixed with symmetrical connecting frames; the side walls of the connecting frames are fixed with fixed frames; and the side walls of the fixed frame are fixed with multiple groups of cleaning brushes.

[0008] Preferably, a sealing ring is provided on the inner side of the cover body; the sealing ring and the cover body are connected by bonding.

[0009] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides a through-hole structure, which is not only simple and convenient to operate, but also can reduce the contact between the bottom of the culture dish and the contact surface when the culture dish body is in use, thereby reducing the impact on heat transfer, making the culture dish body heated more evenly, and can squeeze and fix culture dish bodies of different sizes and models, thereby reducing the shaking of the culture dish body.

[0010] 2. The present invention provides a placement box structure, which is designed to separate the interior of the culture dish, reduce mutual interference between different bacteria, and enable multiple bacteria to be detected at the same time, thereby reducing the workload of the staff and making it easy for the staff to install and disassemble the placement box, thereby making it easy for the staff to clean and disinfect the placement box. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The present invention will be further described below in conjunction with the accompanying drawings.

[0012] Figure 1 It is a stereogram in the present invention; Figure 2 It is a structural schematic diagram of the placement box in the present invention; Figure 3 It is a schematic diagram of the structure of the cleaning brush of the present invention; Figure 4 It is a structural schematic diagram of the sealing ring in the present invention; Figure 5 It is a schematic flow chart of the manufacturing method of the present invention; Figure 6 It is a schematic flow chart of the method of using the present invention.

[0013] The serial numbers in the figure are: 1. Culture dish body; 10. Support frame; 11. Support leg; 12. Threaded rod; 13. Handle; 14. Push plate; 15. Guide rod; 16. Spring rod; 17. Rubber block; 18. Support rod; 19. Support plate; 110. Through hole; 111. Cover; 2. Mounting groove; 21. Mounting block; 22. Placement box; 23. Marking column; 3. Connecting frame; 31. Fixed frame; 32. Cleaning brush; 4. Sealing ring. DETAILED DESCRIPTION

[0014] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods. Example 1

[0015] A culture dish used for bacterial drug sensitivity testing, such as Figure 1 - Figure 4As shown, it includes a culture dish body 1; a support frame 10 is arranged on the outer side of the culture dish body 1; the side wall of the support frame 10 is symmetrically fixed with legs 11; the side wall of the support frame 10 is symmetrically provided with threaded rods 12; the threaded rods 12 penetrate the surface of the support frame 10 and are threadedly connected thereto; the end of the threaded rod 12 is fixedly connected with a handle 13; the end of the threaded rod 12 is rotatably connected with a push plate 14; the side wall of the push plate 14 is symmetrically fixed with a guide rod 15; the end of the guide rod 15 is fixedly connected to the side wall of the support frame 10; the side wall of the push plate 14 is fixedly connected with multiple groups of spring rods 16; The end of the spring rod 16 is fixedly connected with a rubber block 17; the rubber block 17 is arranged in an arc shape; a plurality of support rods 18 are arranged at the bottom end of the support frame 10; a support plate 19 is arranged on the side wall of the support rod 18; a plurality of through holes 110 are opened on the side wall of the support plate 19; the through holes 110 penetrate the interior of the support plate 19; a cover 111 is arranged on the top of the culture dish body 1; when working, the support frame 10 can be connected through the legs 11, the support frame 10 can be connected with the support rods 18, the support plate 19 can be connected with the support rods 18, and the culture dish body 1 is placed by the staff The culture dish body 1 is put into the support plate 19, and then the staff rotates the handle 13, and the handle 13 can drive the threaded rod 12 to rotate, and the threaded rod 12 can pass through the support frame 10 so as to drive the push plate 14 to move, and the push plate 14 is moved, so as to drive the spring rod 16 to move, and the spring rod 16 is moved, so as to drive the rubber block 17 to squeeze and fix the culture dish body 1. At the same time, the guide rod 15 can play a connecting and guiding role for the push plate 14. After the fixation is completed, when the staff needs to put the culture dish body 1 into the constant temperature incubator, the staff covers the cover 111 on the culture dish body 1, so that it can play a sealing role, and the heat can be spread more evenly through the through hole 110. This design is not only simple and convenient to operate, but also when the culture dish body 1 is in use, the contact between the bottom of the culture dish and the contact surface can be reduced, thereby reducing the influence on heat transfer, making the culture dish body 1 more evenly heated, and can squeeze and fix culture dish bodies 1 of different sizes and models, thereby reducing the shaking of the culture dish body 1.

[0016] like Figure 1 - Figure 3As shown, the side wall of the culture dish body 1 is provided with multiple groups of mounting grooves 2; the inner side of the mounting groove 2 is slidably connected with a mounting block 21; the mounting block 21 is connected to the mounting groove 2 in a sliding manner; the side wall of the mounting block 21 is fixedly connected with a placement box 22; the side wall of the culture dish body 1 is fixedly connected with multiple groups of marking columns 23; during operation, multiple bacteria can be detected simultaneously by setting multiple groups of placement boxes 22, and different bacteria can be annotated by the marking columns 23, and the staff can remove the mounting block 21 from the mounting groove 2, so that the placement box 22 can be disassembled, and the staff can insert the mounting block 21 into the mounting groove 2, so that the placement box 22 can be installed. Through this design, the interior of the culture dish can be separated, and the mutual interference between different bacteria can be reduced, so that multiple bacteria can be detected at the same time, thereby reducing the work intensity of the staff, and it is convenient for the staff to install and disassemble the placement box 22, so that it is convenient for the staff to clean and disinfect the placement box 22.

[0017] like Figure 1 and Figure 3 As shown, the side walls of the support frame 10 are symmetrically fixed with symmetrical connecting frames 3; the side walls of the connecting frames 3 are fixed with fixed frames 31; and the side walls of the fixed frames 31 are fixed with multiple groups of cleaning brushes 32; when working, the fixed frames 31 can be supported by the connecting frames 3, and the cleaning brushes 32 can be connected through the fixed frames 31. When the staff puts the culture dish body 1 into the support frame 10 and takes it out, the outer surface of the culture dish body 1 can be cleaned by the cleaning brush 32. With this design, when the staff takes the culture dish body 1, the outer surface of the culture dish body 1 can be cleaned by the cleaning brush 32, thereby reducing the impurities adhering to the outer surface of the culture dish body 1, making it easier for the staff to observe the inside of the culture dish body 1.

[0018] like Figure 4 As shown, a sealing ring 4 is provided on the inner side of the cover body 111; the sealing ring 4 and the cover body 111 are connected by bonding; when working, the sealing ring 4 can improve the sealing performance. This design can improve the sealing performance, thereby reducing the influence of bacteria in the air on the experimental objects inside the culture dish body 1.

[0019] Working principle of the culture dish used for bacterial susceptibility testing: The support frame 10 can be connected through the support leg 11, the support frame 10 can be connected to the support rod 18, the support plate 19 can be connected through the support rod 18, the staff puts the culture dish body 1 on the support plate 19, and then the staff rotates the handle 13, and the handle 13 can drive the threaded rod 12 to rotate, and the threaded rod 12 can pass through the support frame 10 so that the push plate 14 can be driven to move, and the push plate 14 can be moved to drive the spring rod 16 to move, and the spring rod 16 can be moved to drive the rubber block 17 to squeeze and fix the culture dish body 1. At the same time, the guide rod 15 can play a role of connecting and guiding the push plate 14. After the fixation is completed, when the staff needs to put the culture dish body 1 into the constant temperature incubator, through The staff covers the cover 111 on the culture dish body 1 so that it can play a sealing role. The through hole 110 can make the heat spread more evenly. By setting up multiple groups of placement boxes 22, multiple bacteria can be detected simultaneously, and different bacteria can be annotated through the marking column 23. The staff takes out the mounting block 21 from the mounting groove 2, so that the placement box 22 can be disassembled. The staff inserts the mounting block 21 into the mounting groove 2, so that the placement box 22 can be installed. The connecting frame 3 can support the fixing frame 31, and the fixing frame 31 can be connected to the cleaning brush 32. When the staff puts the culture dish body 1 into the supporting frame 10 and takes it out, the cleaning brush 32 can clean the outer surface of the culture dish body 1, and the sealing ring 4 can improve the sealing performance.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: Beneficial effects 1. The through-hole structure provided in the present invention is not only simple and convenient to operate, but also can reduce the contact between the bottom of the culture dish and the contact surface when the culture dish body is in use, thereby reducing the impact on heat transfer and making the culture dish body heated more evenly. It can also squeeze and fix culture dish bodies of different sizes and models, thereby reducing the shaking of the culture dish body.

[0021] Beneficial effect 2. The present invention provides a placement box structure, and this design can separate the interior of the culture dish, which can reduce the mutual interference between different bacteria, so that multiple bacteria can be detected at the same time, thereby reducing the workload of the staff, and making it easy for the staff to install and disassemble the placement box, thereby facilitating the staff to clean and disinfect the placement box. Example 2

[0022] A method for preparing a culture dish for bacterial drug sensitivity test comprises the following steps: S1. Raw material preparation: prepare high-quality polystyrene particles or powder as the main raw material for making culture dishes, ensure that the raw materials are free of impurities, endotoxins, heat sources, and DNA / RNA enzymes, and prepare the mold; S2, injection molding: polystyrene particles or powder are heated to a molten state to make it fluid, and the molten polystyrene material is injected into a mold, which is a mold with the same shape as the culture dish, and maintained at a certain pressure and temperature for a period of time to allow the material to fully fill the mold cavity to form the main shape of the culture dish, and then the mold is cooled; S3, demoulding process: when the culture dish is completely solidified in the mold, the mold is opened and the molded culture dish is taken out of the mold; S4, post-processing: trimming and grinding the culture dish after demoulding, removing excess burrs and scraps, making the edge of the culture dish smooth and flat, and then cleaning and disinfecting the culture dish; S5. Quality inspection: Conduct quality inspection on the finished culture dishes, including appearance inspection, size measurement, and sealing test.

[0023] like Figure 5 As shown, according to S2, maintaining a certain pressure and temperature for a period of time means that the temperature is usually between 180°C and 250°C, the pressure is generally between 50MPa and 150MPa, and the holding time is usually from several seconds to tens of seconds.

[0024] like Figure 5 As shown, the quality inspection proposed in S4 refers to the appearance inspection mainly checking whether the culture dish has cracks, bubbles, and deformation defects; the size measurement mainly checks whether the diameter, height, thickness and other dimensions of the culture dish meet the design requirements; the sealing test mainly checks whether the sealing performance between the lid and the main body of the culture dish is good.

[0025] Compared with the prior art, the method for making a culture dish of the present invention has the following beneficial effects: Beneficial effects 1. The present invention can reduce the uncertainty in the experiment by ensuring that the raw materials are free of impurities, endotoxins, pyrogens, and DNA / RNA enzymes during production, so that different batches of experiments can obtain similar results under the same conditions, thereby improving the repeatability and scientificity of the experiment.

[0026] Beneficial effect 2. The present invention can make the surface of the culture dish smoother through post-processing, reduce microscopic unevenness, and the flat surface helps cells to adhere and grow evenly, avoiding uneven cell distribution or poor local growth due to uneven surface. Example 3

[0027] A method for using a culture dish for bacterial drug sensitivity testing comprises the following steps: A1. Sample preparation: Ensure the operating environment is clean, use appropriate disinfectants to disinfect the work surface and laboratory equipment, and at the same time, check the culture dish itself to ensure that it is not damaged or contaminated, and prepare the culture medium at the same time; A2. Inoculation of bacteria: Bacterial sample collection: Collect an appropriate amount of bacterial samples from clinical samples such as blood, urine, sputum or pure cultured bacterial strains. Use sterile cotton swabs and inoculation loop tools to collect the samples. Spread the collected bacterial samples evenly on the surface of the culture medium under sterile conditions. Ordinary nutrient agar culture medium can be purchased at a biochemical reagent store. Different culture media can be selected for drug sensitivity tests of different bacteria. For example, ordinary nutrient agar or wheat bran Kai culture medium can be selected for drug sensitivity tests of Escherichia coli. Serum culture medium can be selected for Salmonella.

[0028] A3. Place drug-sensitive paper: According to the purpose of the test and the type of bacteria, select the appropriate drug-sensitive paper and place it accurately on the surface of the culture medium. Preparation method of drug-sensitive paper: Take Xinhua No. 1 qualitative filter paper and use a puncher to punch it into a small round paper with a diameter of 6 mm. Take 50 round paper pieces and put them into a clean and dry empty penicillin bottle, and wrap the bottle mouth with a single layer of kraft paper. After high-pressure sterilization at 15 pounds for 15-20 minutes, place it in a 37℃ incubator or oven for several days to completely dry. Preparation of antibacterial paper: Add 0.25 ml of drug solution to the above penicillin bottle containing 50 paper pieces, and turn the paper pieces to make each paper piece fully soaked with the drug solution. Do not smash the paper pieces when turning them over. At the same time, record the name of the drug on the bottle mouth, place it in a 37℃ incubator overnight, cover it tightly after drying, and vacuum dry it if conditions permit.

[0029] A4. Cultivation and observation: Place the culture dish inoculated with bacteria and drug-sensitive paper in a constant temperature incubator for cultivation. The culture temperature is generally 35-37°C. The culture time depends on the different bacterial species and test requirements, usually 18-24 hours. Regularly observe the bacterial growth in the culture dish and the antibacterial zone around the drug-sensitive paper. A5. Result analysis: The results of the drug sensitivity test shall be presented in the form of a report, including the type of bacteria, the name of the drug on the drug sensitivity paper, the diameter of the inhibition zone, and the information on the sensitivity of the bacteria to the drug. The culture dishes shall be processed. After the test, the used culture dishes shall be placed in a special medical waste container and handled in accordance with the relevant regulations on medical waste disposal.

[0030] like Figure 6As shown, the culture medium preparation proposed in A1 refers to preparing a suitable bacterial culture medium, such as agar culture medium, according to the test requirements, heating the culture medium until it is completely dissolved, and then pouring it into the culture dish under sterile conditions so that it is evenly distributed on the bottom of the culture dish, with a thickness of generally 3-5 mm. After the culture medium is cooled and solidified, it is ready for use.

[0031] Table 1 Criteria for drug sensitivity testing Diameter of inhibition zone (mm) Sensitivity 20 and above Extremely sensitive 15~20 High sensitivity 10~14 Zhongmin Below 10 Hypoallergenic 0 Insensitive .

[0032] After the drug sensitivity test, highly sensitive drugs should be selected for treatment, and two drugs can also be used together to reduce drug-resistant strains. When choosing highly sensitive drugs, the absorption pathway of the drugs should be considered, because our drug sensitivity test is the direct contact between the drug solution and the bacteria, and when the drug is given to living animals, it must be absorbed by the body to achieve a certain effect. Therefore, when giving drugs to living animals, highly sensitive drugs must be used in conjunction with appropriate administration methods to achieve good therapeutic effects.

[0033] Compared with the prior art, the method for using the culture dish of the present invention has the following beneficial effects: Beneficial effects 1. The present invention can reduce the phenomenon of damage to the culture dish by inspecting the culture dish itself before use, thereby reducing the leakage of bacteria from the damaged part, thereby reducing safety hazards.

[0034] Beneficial Effect 2. The present invention can avoid the spread of bacteria and viruses by processing the culture dish after use, and through the correct processing method, the waste in the culture dish can be classified, disinfected or harmlessly treated, reducing the risk of pollution to the soil, water and air.

[0035] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A culture dish for use in bacterial drug sensitivity testing, comprising a culture dish body (1); characterized in that: A support frame (10) is arranged on the outside of the culture dish body (1); legs (11) are symmetrically fixedly connected to the side walls of the support frame (10); threaded rods (12) are symmetrically arranged on the side walls of the support frame (10); the threaded rods (12) penetrate the surface of the support frame (10) and are threadedly connected thereto; a handle (13) is fixedly connected to the end of the threaded rod (12); a push plate (14) is rotatably connected to the end of the threaded rod (12); a guide rod (15) is symmetrically fixedly connected to the side walls of the push plate (14); the end of the guide rod (15) is rotatably connected to the support frame The push plate (14) is fixedly connected to the side wall of the push plate (14); a plurality of spring rods (16) are fixedly connected to the end of the spring rod (16); the rubber block (17) is arranged in an arc shape; a plurality of support rods (18) are arranged at the bottom end of the support frame (10); a support plate (19) is arranged on the side wall of the support rod (18); a plurality of through holes (110) are arranged on the side wall of the support plate (19); the through holes (110) penetrate the interior of the support plate (19); and a cover (111) is arranged at the top end of the culture dish body (1).

2. A culture dish for bacterial drug sensitivity testing according to claim 1, characterized in that: The side wall of the culture dish body (1) is provided with a plurality of mounting grooves (2); a mounting block (21) is slidably connected to the inner side of the mounting groove (2); the mounting block (21) is connected to the mounting groove (2) in a slidable manner; a placement box (22) is fixedly connected to the side wall of the mounting block (21); and a plurality of marking columns (23) are fixedly connected to the side wall of the culture dish body (1).

3. A culture dish for bacterial drug sensitivity test according to claim 1, characterized in that: A symmetrical connecting frame (3) is symmetrically fixedly connected to the side wall of the support frame (10); a fixed frame (31) is fixedly connected to the side wall of the connecting frame (3); and a plurality of groups of cleaning brushes (32) are fixedly connected to the side wall of the fixed frame (31).

4. A culture dish for bacterial drug sensitivity test according to claim 1, characterized in that: A sealing ring (4) is provided on the inner side of the cover body (111); the sealing ring (4) and the cover body (111) are connected by bonding.

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