Nanometer adhesive tape for surface enhanced Raman scattering detection and preparation method and application thereof

Abstract

The invention relates to a nano adhesive tape for surface enhanced Raman scattering detection, a method for preparing the nano adhesive tape for surface enhanced Raman scattering detection and application of the nano adhesive tape in preparation of an in-vitro diagnosis and detection kit, a food sanitation and safety detection kit or a water resource pollution detection kit. The nano adhesive tape comprises a flexible insulator film, a metal film deposited on the flexible insulator film, nano metal thorns deposited on the metal film, and a Raman tag modified on the nano metal thorns and the metal film. According to the technical scheme, the nano adhesive tape disclosed by the invention has flexibility and can be attached to any curved surface, so that the detection sensitivity of a Raman enhanced detection chip can be remarkably improved when the nano adhesive tape is matched with the curved surface which is more beneficial to Raman scattering detection.

Description

Technical Field

[0001] The present invention relates to the field of nanomaterials, and in particular, to a nano tape for surface enhanced Raman scattering detection, a method for preparing the nano tape for surface enhanced Raman scattering detection, and use of the nano tape in preparing an in vitro diagnostic test kit, a food hygiene and safety test kit, or a water resource pollution test kit. Background Art

[0002] With the rapid technological development and economic growth, the concern for public health issues such as biomedicine, food safety and environmental pollution has led to an increasing demand for rapid and portable sensing technologies. SERS substrate materials with good stability, high sensitivity and ultra-clean surface are essential for the practical use of biochemical sensing, especially for point-of-care testing (POCT). As a rapid and portable sensing technology, point-of-care testing has the advantages of high precision and high sensitivity, and plays an important role in the field of in vitro diagnosis. While preparing SERS detection chips conveniently and effectively, we constructed gold nanothorn (AuNT) flexible film nanotape based on surface Raman enhancement technology, which can fit human skin for clinical point-of-care testing in biomedicine. Using the AuNT ultra-clean surface flexible SERS chip obtained instantly, high-sensitivity detection can be achieved, including food hygiene and safety, biomedicine and water resource pollution monitoring.

[0003] CN202010650843.1 discloses a method for preparing a gold nanothorn Raman enhanced detection chip, which includes: preparing a chip substrate with a clean surface; spraying a layer of platinum particles on the surface of the chip substrate by ion sputtering; dripping silver nitrate solution and chloroauric acid solution on the chip substrate, and then dripping hydrogen peroxide for reduction; after the reaction in step S3 is completed, washing the chip with deionization to obtain the gold nanothorn Raman enhanced detection chip.

[0004] However, the detection sensitivity of existing Raman enhanced detection chips still needs to be improved. Summary of the invention

[0005] The purpose of the invention is to improve the detection sensitivity of the Raman enhanced detection chip.

[0006] In order to achieve the above-mentioned object, the present invention provides a nano tape for surface enhanced Raman scattering detection, which comprises a flexible insulator film, a metal film deposited on the flexible insulator film, nano metal spikes precipitated on the metal film, and Raman tags modified on the nano metal spikes and the metal film.

[0007] Optionally, the flexible insulator film is a polydimethylsiloxane film.

[0008] Optionally, the thickness of the flexible insulator film is 0.1-5 mm.

[0009] Optionally, the metal film is a Pt film.

[0010] Optionally, the metal film is a nano Pt film obtained by sputtering.

[0011] Optionally, the Raman tag is docosapentaenol.

[0012] Optionally, the nano metal thorns are thorn-shaped nano gold and thorn-shaped nano silver.

[0013] Optionally, the molar ratio between the gold element in the thorn-shaped nanogold and the silver element in the thorn-shaped nanosilver is 5-20:1.

[0014] The present invention also provides a method for preparing a nano-tape for surface-enhanced Raman scattering detection, the method comprising: S1, depositing a metal film on a flexible insulator film to obtain a first flexible substrate; S2, dripping a metal source solution and a precipitant on the flexible substrate to precipitate and generate nano-metal thorns to obtain a second flexible substrate; S3, modifying a Raman tag on the second flexible substrate to obtain a nano-tape for surface-enhanced Raman scattering detection.

[0015] The present invention also provides the use of the nano tape as described above in preparing an in vitro diagnostic test kit, a food hygiene and safety test kit or a water resource pollution test kit.

[0016] Through the above technical solution, the nano tape of the present invention is flexible and can fit any curved surface. Therefore, when used with a curved surface that is more conducive to Raman scattering detection, the detection sensitivity of the Raman enhanced detection chip can be significantly improved.

[0017] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention, but do not constitute a limitation of the present invention. In the accompanying drawings:

[0019] Figure 1 It is a picture of the preferred flexible nano tape of the present invention.

[0020] Figure 2 It is a schematic diagram of the structure of a preferred flexible nano tape of the present invention.

[0021] Description of Reference Numerals

[0022] 1 Flexible insulator film

[0023] 2 metal film

[0024] 3 nano metal spikes DETAILED DESCRIPTION

[0025] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0026] The invention provides a nano tape for surface enhanced Raman scattering detection. The nano tape comprises a flexible insulating film, a metal film deposited on the flexible insulating film, nano metal spikes precipitated on the metal film, and Raman labels modified on the nano metal spikes and the metal film.

[0027] In the present invention, the flexible insulator film has the function of supporting the metal film, the nano-metal spikes and the Raman tag, and can be attached to any curved surface due to its flexibility, so when used with a curved surface that is more conducive to Raman scattering detection, the detection sensitivity of the Raman enhanced detection chip can be significantly improved. The metal film can provide a binding substrate for the nano-metal spikes. The nano-metal spikes can enhance the Raman signal. The Raman tag can be used as a marker for qualitative or quantitative detection.

[0028] Optionally, the flexible insulator film may be a polydimethylsiloxane film and / or a polyimide film, preferably a polydimethylsiloxane film.

[0029] Optionally, the thickness of the flexible insulator film may be 0.1-5 mm, preferably 0.5-3 mm.

[0030] Optionally, the metal film may be at least one of a Pt film, an Au film and an Ag film.

[0031] Optionally, the metal film may be at least one of a nano-Pt film, a nano-Au film and a nano-Ag film obtained by sputtering.

[0032] Optionally, the Raman tag may be docosapentaenol (DPA) or parathioaniline (PATP). Docosapentaenol (DPA) may be used as a Bacillus biomarker.

[0033] Optionally, the nano metal thorns may be a mixture of thorn-shaped nano gold and thorn-shaped nano silver. The nano metal thorns may be prepared by precipitation.

[0034] Optionally, the molar ratio between the gold element in the thorn-shaped nanogold and the silver element in the thorn-shaped nanosilver may be 5-20:1, preferably 10-15:1.

[0035] The present invention also provides a method for preparing a nano-tape for surface-enhanced Raman scattering detection, the method comprising: S1, depositing a metal film on a flexible insulator film to obtain a first flexible substrate; S2, dripping a metal source solution and a precipitant on the flexible substrate to precipitate and generate nano-metal thorns to obtain a second flexible substrate; S3, modifying a Raman tag on the second flexible substrate to obtain a nano-tape for surface-enhanced Raman scattering detection.

[0036] In the above method of the present invention, the metal film can be deposited on the flexible insulator film by ion sputtering. For example, in a vacuum container, under the action of high voltage (such as 1200-2000V), the residual metal gas is ionized to form plasma, and the positive ions bombard the metal target under the acceleration of the electric field, so that the metal atoms are sputtered to the surface of the sample to form a conductive film.

[0037] Wherein, the metal source solution may include AgNO3 solution and HAuCl4 solution, and the precipitant may include H2O2 solution. The precipitant reduces the metal Ag and Au in the metal source solution to precipitate and generate nano-metal thorns. The concentration of the AgNO3 solution may be 1-100mM, preferably 5-20mM, and the concentration of the HAuCl4 solution may be 1-200mM, preferably 20-100mM. The concentration of the H2O2 solution may be 1-100nM.

[0038] The present invention also provides the use of the nano tape as described above in preparing an in vitro diagnostic test kit, a food hygiene and safety test kit or a water resource pollution test kit.

[0039] The present invention is further described in detail below by way of examples.

[0040] Example 1

[0041] Preparation and characterization of nanotapes for surface-enhanced Raman scattering detection.

[0042] Step S1, firstly, a layer of metal Pt is deposited on a clean PDMS film, and the ion sputtering time is 20-100s.

[0043] Step S2, add AgNO3 solution (20μL, 10mM) and HAuCl4 solution (60μL, 50mM) in sequence, and after the solution is spread on the glass slide, add H2O2 solution to produce nano-metal thorns. The reaction time is about 20s. Rinse with a large amount of deionized water and air-dry to obtain a flexible AuNT Raman enhanced chip with an ultra-clean surface; use a flexible AuNT nanochip.

[0044] Step S3, 20 μL of an aqueous solution containing the Bacillus biomarker DPA (0.1-1000 ppb) and animal serum are added dropwise to the previously prepared SERS chip, and dried at 37° C., thereby completing the preparation of the DPA-modified AuNT chip.

[0045] Finally, SERS detection was performed using a Raman spectrometer.

[0046] The AuNT chip is flexible and can be attached to a curved surface, which can significantly improve the detection sensitivity of the Raman enhanced detection chip compared to a flat surface.

[0047] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0048] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0049] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A nano tape for surface enhanced Raman scattering detection, characterized in that: The nano adhesive tape comprises a flexible insulating film, a metal film deposited on the flexible insulating film, nano metal spikes precipitated on the metal film, and Raman labels modified on the nano metal spikes and the metal film.

2. The nano tape according to claim 1, wherein The flexible insulator film is a polydimethylsiloxane film.

3. The nano tape according to claim 1 or 2, wherein The thickness of the flexible insulator film is 0.1-5 mm.

4. The nano tape according to claim 1, wherein The metal film is a Pt film.

5. The nano tape according to claim 1 or 4, wherein The metal film is a nano Pt film obtained by sputtering.

6. The nano tape according to claim 1, wherein The Raman tag is docosapentaenol.

7. The nano tape according to claim 1, wherein The nano metal thorns are thorn-shaped nano gold and thorn-shaped nano silver.

8. The nano tape according to claim 7, wherein: The molar ratio between the gold element in the thorn-shaped nanogold and the silver element in the thorn-shaped nanosilver is 5-20:

1.

9. A method for preparing a nanotape for surface enhanced Raman scattering detection, characterized in that: The method includes: S1, depositing a metal film on a flexible insulator film to obtain a first flexible substrate; S2, adding a metal source solution and a precipitant dropwise onto the flexible substrate to precipitate and generate nano-metal thorns to obtain a second flexible substrate; S3, modifying the Raman label on the second flexible substrate to obtain a nanotape for surface enhanced Raman scattering detection.

10. Use of the nano tape according to any one of claims 1 to 8 in the preparation of an in vitro diagnostic test kit, a food hygiene and safety test kit or a water resource pollution test kit.

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