Auxiliary device for realizing simultaneous single-photon and double-photon in-vivo imaging of brain and spinal cord of mouse

Abstract

The invention discloses an auxiliary device for achieving simultaneous single-photon and double-photon in-vivo imaging of the brain and the spinal cord of a mouse, and relates to the technical field of experimental devices.The auxiliary device comprises a brain imaging auxiliary plate, a spine clamping piece, a spinal cord imaging auxiliary plate and an adjustable telescopic piece, and a first water containing groove is formed in the middle of the brain imaging auxiliary plate; the brain imaging auxiliary part is adhered and fixed to a mouse brain; the spine clamping piece comprises a clamping bottom plate and a pair of clamping plate assemblies, the clamping bottom plate comprises a first bottom plate and a second bottom plate which are connected in an inserted mode, the clamping plate assemblies are arranged on the first bottom plate and the second bottom plate respectively, and the clamping plate assemblies can get close to each other in the same direction and get away from each other in the opposite directions to clamp and loosen the spine of the mouse; the spinal cord imaging auxiliary plate is detachably arranged on the clamping plate assembly, and a second water containing groove is formed in the middle of the spinal cord imaging auxiliary plate; the adjustable telescopic piece is connected to the brain imaging auxiliary plate and the spinal cord imaging auxiliary plate. According to the method, groove milling in the skull and spine of the mouse can be avoided, and the operation difficulty and the operation risk are greatly reduced.

Description

Technical Field

[0001] The present application relates to the technical field of experimental devices, and in particular to an auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord. Background Art

[0002] When performing single- and double-photon imaging of the mouse brain and spinal cord, the current method is to use a milling cutter to mill grooves on the mouse's skull and spine, respectively, and then fill the grooves with water and use an imaging instrument to perform imaging.

[0003] The traditional operation method has high requirements on the experimental personnel. The slightest negligence can easily damage the mouse's brain and spine. It has the disadvantages of high risk factor and great difficulty in operation. Summary of the invention

[0004] The main purpose of the present application is to provide an auxiliary device for achieving simultaneous single-photon and double-photon in vivo imaging of the mouse brain and spinal cord, aiming to solve the above-mentioned problems.

[0005] The technical solutions adopted in this application are as follows:

[0006] An auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord, comprising:

[0007] A brain imaging auxiliary board, wherein a first water tank is centrally arranged on the brain imaging auxiliary board, and the brain imaging auxiliary piece is adhesively fixed to the mouse brain;

[0008] A spine clamp, the spine clamp comprising a clamping base plate and a pair of clamping plate assemblies, the clamping base plate comprising a first base plate and a second base plate plugged into each other, the first base plate and the second base plate are respectively provided with the clamping plate assemblies, and the clamping plate assemblies can approach and move away in the same direction to clamp and release the mouse spine;

[0009] A spinal cord imaging auxiliary board, the spinal cord imaging auxiliary board is detachably arranged on the clamping plate assembly, and a second water tank is centrally arranged on the spinal cord imaging auxiliary board;

[0010] An adjustable telescopic member is connected to the brain imaging auxiliary plate and the spinal cord imaging auxiliary plate.

[0011] Optionally, the splint assembly comprises:

[0012] An L-shaped slide, the L-shaped slide is fixedly arranged on the clamping bottom plate, and a slide groove is arranged on the horizontal section of the L-shaped slide;

[0013] A slider, the slider is slidably disposed in the slide groove, and the slider is provided with a positioning bolt for locking the slider in the slide groove;

[0014] A clamping block is arranged at the front end of the sliding block, and a dovetail clamping groove is arranged at one end of the clamping block away from the sliding block.

[0015] Optionally, the clamping block and the sliding block are detachably connected via a countersunk bolt.

[0016] Optionally, the spinal cord imaging auxiliary plate is detachably connected to the slider via a fastening bolt.

[0017] Optionally, the spinal cord imaging auxiliary plate is provided with strip grooves on both sides of the second water tank, and the fastening bolts can move in the strip grooves.

[0018] Optionally, the adjustable telescopic member includes:

[0019] A mounting plate, one mounting plate is provided on each of the brain imaging auxiliary plate and the spinal cord imaging auxiliary plate, and the mounting plate is provided with a card slot along the longitudinal direction;

[0020] A first rod, one end of which is slidably disposed in a slot where a mounting plate on the brain imaging auxiliary plate is located, and the other end of the first rod is provided with a step shaft, and the step shaft is provided with a spring pin;

[0021] A second rod member, one end of which is slidably disposed in a slot where a mounting plate on the spinal cord imaging auxiliary plate is located, the other end of which is provided with a socket for inserting the step shaft, and a plurality of positioning pin holes are disposed on an outer wall of the second rod member, and the spring pin can be inserted into different positioning pin holes.

[0022] Optionally, a recessed groove is provided on the side of the first bottom plate facing the second bottom plate, an insert block inserted into the recessed groove is provided on the side of the second bottom plate facing the first bottom plate, limiting grooves are provided on both sides of the recessed groove, and L-shaped spring clips that are inserted into the limiting grooves are provided on both sides of the insert block.

[0023] Compared with the prior art, the beneficial effects of this application are:

[0024] An auxiliary device for achieving simultaneous single- and double-photon in vivo imaging of a mouse brain and spinal cord is proposed in an embodiment of the present application. By setting a brain imaging auxiliary plate, a spine clamp and a spinal cord imaging auxiliary plate, the brain imaging plate is fitted and fixed to the mouse brain, the spine clamp is clamped by the mouse spine, and the spinal cord imaging auxiliary plate is fixed on the spine clamp to fit the spinal cord imaging auxiliary plate to the mouse spine. The first water tank and the second water tank are used to hold water, thereby avoiding milling grooves on the mouse skull and spine, greatly reducing the difficulty and risk of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1A schematic diagram of the structure of an auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of the mouse brain and spinal cord provided in an embodiment of the present application at one viewing angle;

[0026] Figure 2 A schematic diagram of the structure of the auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of the mouse brain and spinal cord provided in an embodiment of the present application from another perspective;

[0027] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0028] Figure 4 for Figure 2 Enlarged view of point B

[0029] Figure 5 for Figure 4 A cross-sectional view of

[0030] Figure 6 A cross-sectional view of the clamping base plate.

[0031] Description of the reference numerals in the accompanying drawings:

[0032] 1-brain imaging auxiliary plate, 101-first water tank, 2-spinal clamp, 201-clamping base plate, 2010-first base plate, 2011-second base plate, 2012-insertion block, 2013-L-shaped spring clip, 202-L-shaped slide, 203-slider, 204-positioning bolt, 205-clamping block, 206-countersunk bolt, 3-spinal cord imaging auxiliary plate, 301-second water tank, 302-strip groove, 4-fastening bolt, 5-adjustable telescopic part, 501-first rod, 502-second rod, 503-step shaft, 504-spring pin, 505-positioning pin hole, 506-mounting plate, 507-slot, 508-locking nut. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0034] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0035] In this application, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0036] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0037] See attached Figures 1 to 3 The embodiment of the present application provides an auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of the mouse brain and spinal cord, including a brain imaging auxiliary board 1, a spine clamp 2, a spinal cord imaging auxiliary board 3, and an adjustable telescopic part 5. Among them, the brain imaging auxiliary board 1 is a square strip board, a first water tank 101 is arranged in the center of the brain imaging auxiliary board 1, and U-shaped bayonet is arranged on both sides. The U-shaped bayonet is used to fix the brain imaging auxiliary board 1 to the base buckle of the imaging instrument during imaging. The brain imaging auxiliary part is fixed to the mouse brain by structural adhesive, and the first water tank 101 is sealed around the mouse brain by structural adhesive to prevent water seepage.

[0038] As attached Figures 1 to 3 As shown, the spine clamp 2 includes a clamping base plate 201 and a pair of clamping plate assemblies.

[0039] The clamping plate assembly is arranged symmetrically with the center of the clamping base plate 201, and the clamping plate assemblies on both sides can approach and move away from each other in the same direction to clamp and release the mouse spine. When in use, the clamping base plate 201 is placed under the spine, and the clamping plate assemblies on both sides are adjusted to clamp the mouse spine.

[0040] In a preferred embodiment, in order to facilitate placing the clamping base plate 201 under the spine, see Figure 6As shown, the clamping base plate 201 includes a first base plate 2010 and a second base plate 2011, a sink groove is provided on the side of the first base plate 2010 facing the second base plate 2011, and an insert block 2012 inserted into the sink groove is provided on the side of the second base plate 2011 facing the first base plate 2010, and limiting grooves are provided on both sides of the sink groove, and L-shaped spring pieces 2013 inserted into the limiting grooves are provided on both sides of the insert block 2012. In the process of placing the clamping base plate 201 under the spine, the first base plate 2010 and the second base plate 2011 are first disassembled and then placed on both sides of the spine, and the first base plate 2010 and the second base plate 2011 are pushed toward the middle so that the insert block 2012 is inserted into the sink groove, and the L-shaped spring piece 2013 enters the sink groove simultaneously, and when it is opposite to the limiting groove, it pops into the limiting groove and clamps, thereby conveniently placing the clamping base plate 201 under the spine. If the clamping base plate 201 needs to be removed, the L-shaped spring piece 2013 only needs to be pressed inward to make the L-shaped spring piece 2013 withdraw from the limiting groove, and then the first base plate 2010 and the second base plate 2011 are pulled to both sides to be removed from under the spine, which is very convenient.

[0041] Meanwhile, the first base plate 2010 and the second base plate 2011 are both provided with a clamping plate assembly, specifically, the clamping plate assembly includes an L-shaped slide 202202, a slider 203 and a clamping block 205, wherein the vertical section of the L-shaped slide 202202 is integrally formed with the clamping base plate 201, the horizontal section of the L-shaped slide 202202 is supported from the clamping base plate 201 and remains parallel to the clamping base plate 201, the horizontal section of the L-shaped slide 202202 is provided with a slide groove along the long axis direction of the clamping base plate 201, the slider 203 is slidably installed in the slide groove, and the slider 203 is provided with a positioning bolt 204, which is used to lock the slider 203 at any position of the slide groove. The clamping block 205 is provided at one end of the slider 203 close to the center of the clamping base plate 201, and the clamping block 205 is detachably connected to the slider 203 through a countersunk bolt 206, so as to facilitate the replacement of the clamping block 205 after use. At the same time, a dovetail-shaped clamping groove is designed at one end of the clamping block 205 away from the sliding block 203 to facilitate clamping the mouse spine.

[0042] The above mentioned Figures 1 to 3 As shown, the spinal cord imaging auxiliary board 3 is a square board, and a second water tank 301 is centrally arranged on the spinal cord imaging auxiliary board 3. Meanwhile, the spinal cord imaging auxiliary board 3 is detachably connected to the clamping block 205. Specifically, the spinal cord imaging auxiliary board 3 is provided with strip grooves 302 on both sides of the second water tank 301, and fastening bolts 4 are arranged in the strip grooves 302. Bolt holes for connecting the fastening bolts 4 are arranged on the clamping block 205. After the spinal cord imaging board is connected to the clamping block 205 by the fastening bolts 4, the strip grooves 302 can be used to facilitate the clamping and movement of the clamping block 205.

[0043] At the same time, in order to be able to adjust the distance between the brain imaging auxiliary board 1 and the spinal cord auxiliary imaging board 3 according to mice of different sizes, such as Figures 1 to 5 As shown, an adjustable telescopic member 5 is provided between the brain imaging auxiliary plate 1 and the spinal cord imaging auxiliary plate 3, and the adjustable telescopic member 5 includes a mounting plate 506, a first rod 501 and a second rod 502. Among them, the mounting plate 506 is integrally formed on the sides of the brain imaging auxiliary plate 1 and the spinal cord imaging auxiliary plate 3 that are close to each other, and the mounting plate 506 is provided with a slot 507 along the longitudinal direction; one end of the first rod 501 is slidably provided in the slot 507 where the mounting plate 506 on the brain imaging auxiliary plate is located, and one end of the second rod 502 is slidably provided in the slot 507 where the mounting plate 506 on the spinal cord imaging auxiliary plate is located, and the first rod 501 and the second rod 502 are both fixed to the mounting plate 506 by bolt heads and locking nuts 508. The other end of the first rod 501 is provided with a stepped shaft 503, and a spring pin 504 is provided on the stepped shaft 503; the other end of the second rod 502 is provided with a socket for inserting the stepped shaft 503, and the outer wall of the second rod 502 is provided with a plurality of positioning pin holes 505, and the spring pin 504 can be inserted into different positioning pin holes 505 according to the different lengths of the stepped shaft 503 inserted into the socket. It can be imagined that by providing an adjustable telescopic member 5 between the brain imaging auxiliary board 1 and the spinal cord auxiliary imaging board 3, the entire device is formed into a whole, and by providing a card slot 507 on the mounting seat, the first rod 501 and the second rod 502 can adjust the height as needed to adapt to the height difference between the skull and the spine, and the different depths of the stepped shaft 503 inserted into the socket can adjust the distance between the brain imaging auxiliary board 1 and the spinal cord imaging auxiliary board 3, so that it can be suitable for mice of different sizes.

[0044] In summary, the embodiment of the present application provides an auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of the mouse brain and spinal cord, and the method of using the auxiliary device is as follows:

[0045] First, anesthetize the mouse on a sterile operating laboratory table and cut the subcutaneous tissue of the back and brain to expose the skull and spine; then lift the mouse spine slightly, place the first base plate 2010 and the second base plate 2011 on both sides of the mouse spine, and squeeze the first base plate 2010 and the second base plate 2011 toward the middle to snap them together and integrate them into one. At this time, the clamping base plate is placed under the mouse spine, and then apply structural glue on one side of the spinal cord imaging auxiliary plate 3 and fit it on the clamp block 205. Use the fastening bolts 4 to install the spinal cord imaging auxiliary plate 3 on the clamp block 205, and use the structural glue to fit the spinal cord imaging auxiliary plate 3 to the mouse spine to avoid the first The second water tank 301 leaks water, and then the length between the first rod 501 and the second rod 502 is adjusted to make the brain imaging auxiliary board 1 located on the top of the mouse skull, and the brain imaging auxiliary board 1 is adhered to the mouse head by structural adhesive, so that the first water tank 101 fits the skull on the top of the mouse head, and the gap between the skull and the brain imaging auxiliary board 1 is sealed by structural adhesive to prevent water from leaking into the first water tank 101; finally, after the brain imaging auxiliary board 1 and the spinal cord imaging auxiliary board 3 are installed, the mouse is placed on the base of the imager, water is filled into the first water tank 101 and the second water tank 301, and the image is observed under the imager.

[0046] It can be seen that the embodiment of the present application provides an auxiliary device for realizing simultaneous single- and double-photon in vivo imaging of the mouse brain and spinal cord. By setting a brain imaging auxiliary plate, a spine clamp and a spinal cord imaging auxiliary plate, the brain imaging plate is fitted and fixed to the mouse brain, the spine clamp is clamped by the mouse spine, and the spinal cord imaging auxiliary plate is fixed on the spine clamp to make the spinal cord imaging auxiliary plate fit the mouse spine. The first water tank and the second water tank are used to hold water, thereby avoiding milling grooves on the mouse skull and spine, greatly reducing the difficulty and risk of operation.

[0047] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. An auxiliary device for achieving simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord, characterized in that: include: A brain imaging auxiliary board, wherein a first water tank is centrally arranged on the brain imaging auxiliary board, and the brain imaging auxiliary piece is adhesively fixed to the mouse brain; A spine clamp, the spine clamp comprising a clamping base plate and a pair of clamping plate assemblies, the clamping base plate comprising a first base plate and a second base plate plugged into each other, the first base plate and the second base plate are respectively provided with the clamping plate assemblies, and the clamping plate assemblies can approach and move away in the same direction to clamp and release the mouse spine; A spinal cord imaging auxiliary board, the spinal cord imaging auxiliary board is detachably arranged on the clamping plate assembly, and a second water tank is centrally arranged on the spinal cord imaging auxiliary board; An adjustable telescopic member is connected to the brain imaging auxiliary plate and the spinal cord imaging auxiliary plate.

2. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 1, characterized in that: The splint assembly comprises: An L-shaped slide, the L-shaped slide is fixedly arranged on the clamping bottom plate, and a slide groove is arranged on the horizontal section of the L-shaped slide; A slider, the slider is slidably disposed in the slide groove, and the slider is provided with a positioning bolt for locking the slider in the slide groove; A clamping block is arranged at the front end of the sliding block, and a dovetail clamping groove is arranged at one end of the clamping block away from the sliding block.

3. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 2, characterized in that: The clamping block and the sliding block are detachably connected via countersunk bolts.

4. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 2, characterized in that: The spinal cord imaging auxiliary plate is detachably connected to the sliding block via fastening bolts.

5. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 4, characterized in that: The spinal cord imaging auxiliary plate is provided with strip grooves on both sides of the second water tank, and the fastening bolts can move in the strip grooves.

6. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 1, characterized in that: The adjustable telescopic member comprises: A mounting plate, one mounting plate is provided on each of the brain imaging auxiliary plate and the spinal cord imaging auxiliary plate, and the mounting plate is provided with a card slot along the longitudinal direction; A first rod, one end of which is slidably disposed in a slot where a mounting plate on the brain imaging auxiliary plate is located, and the other end of the first rod is provided with a step shaft, and the step shaft is provided with a spring pin; A second rod member, one end of which is slidably disposed in a slot where a mounting plate on the spinal cord imaging auxiliary plate is located, the other end of which is provided with a socket for inserting the step shaft, and a plurality of positioning pin holes are disposed on an outer wall of the second rod member, and the spring pin can be inserted into different positioning pin holes.

7. The auxiliary device for realizing simultaneous single-photon and double-photon in vivo imaging of mouse brain and spinal cord according to claim 1, characterized in that: A recessed groove is provided on one side of the first bottom plate toward the second bottom plate, an insert block inserted into the recessed groove is provided on one side of the second bottom plate toward the first bottom plate, limiting grooves are provided on both sides of the recessed groove, and L-shaped spring pieces inserted into the limiting grooves are provided on both sides of the insert block.

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