SERS substrate carrying detection device and use method thereof

Abstract

The invention discloses an SERS substrate carrying detection device and a use method thereof, and belongs to the technical field of SERS detection.The SERS substrate carrying detection device comprises a carrying table, a detection disc and a rotating stand, a plurality of substrate containing grooves are formed in the detection disc, the detection disc is connected with the carrying table through a first rotating shaft, and the first rotating shaft is rotationally sleeved with the rotating stand; a supporting rod is arranged on the upper surface of the rotating frame, a crest-shaped arc-shaped pushing block is fixed to the top of the supporting rod, a mounting disc is fixed to one side of the carrying table through a fixing rod, a plurality of upper dropwise adding pipes are fixed to the mounting disc, and lower dropwise adding pipes are detachably connected to the bottom ends of the upper dropwise adding pipes; a pneumatic transmission mechanism is arranged on one side of the upper dripping pipe and is matched with the arc-shaped push block to realize automatic dripping of a solution; automatic dropwise adding of the solution to be detected is achieved, the detection efficiency and accuracy are improved, and use is easy and convenient.

Description

Technical Field

[0001] The present invention relates to the field of SERS detection technology, and in particular to a SERS substrate-mounted detection device and a use method thereof. Background Art

[0002] Surface enhanced Raman spectroscopy (SERS) is a detection technology based on Raman spectroscopy and nanotechnology. With the help of the electromagnetic field enhancement mechanism and chemical enhancement mechanism of the substrate nanomaterial, it shows very high sensitivity and can be used for fingerprint detection of ultra-trace substances. Manufacturing wafer-compatible composite materials and directly integrating them into miniaturized, portable SERS sensors is an important direction for realizing the equipmentization of SERS technology.

[0003] After searching, it was found that the patent with publication number CN207248750U discloses a SERS substrate carrying detection device, wherein a rotating frame is provided on the top of the loading platform, the rotating frame is provided with a vertically arranged rotating shaft and can be rotatably mounted on the loading platform through the rotating shaft, a driving mechanism is connected to the rotating frame for driving the rotating frame to rotate, a limiting structure is provided on the rotating frame, a detection disc is detachably mounted on the rotating frame and rotates with the rotating frame through the limiting structure, a plurality of substrate accommodating grooves are provided on the top of the detection disc, and the plurality of substrate accommodating grooves are distributed along the circumference around the rotating shaft of the rotating frame;

[0004] This patent adopts a rotating sample measurement method, which is more conducive to the rapid detection and combination of a portable Raman instrument on site, but there are still some problems: in this patent, a plurality of substrate holding grooves are opened on the detection disc, and the solution to be tested needs to be dripped into each substrate holding groove during detection. At present, it is generally done by manual dripping one by one, which is not only time-consuming and labor-intensive, but also the solution will be dripped to the outside of the substrate holding groove if there is any negligence, polluting the environment and affecting the accuracy of the detection results. Summary of the invention

[0005] The purpose of the present invention is to provide a SERS substrate-mounted detection device and a method of using the same to solve the following technical problems: The prior art generally uses manual dripping, which is not only time-consuming and labor-intensive, but also causes the solution to be dripped onto the outside of the substrate holding tank if there is any negligence, thereby polluting the environment and affecting the accuracy of the detection results.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A SERS substrate-mounted detection device comprises a loading platform, a detection disc and a rotating frame, wherein a plurality of substrate accommodating grooves are provided on the detection disc, the detection disc is connected to the loading platform via a first rotating shaft, the rotating frame is rotatably mounted on the outside of the first rotating shaft, a support rod is provided on the upper surface of the rotating frame, a wave-shaped arc-shaped push block is fixed on the top of the support rod, a mounting disc is fixed on one side of the loading platform via a fixing rod, a plurality of upper dripping tubes are fixed on the mounting disc, a lower dripping tube is detachably connected to the bottom end of the upper dripping tube, a pneumatic transmission mechanism is provided on one side of the upper dripping tube, and the pneumatic transmission mechanism cooperates with the arc-shaped push block to realize automatic dripping of the solution;

[0008] It also includes a driving mechanism, which drives the first rotating shaft and the rotating frame to rotate respectively.

[0009] As a further solution of the present invention: the pneumatic transmission mechanism includes a sleeve fixed to one side of the upper drip tube through a fixing block, a connecting pipe is connected between the sleeve and the upper drip tube, a first piston is arranged in the sleeve, a driven rod is connected to the bottom of the first piston, a driven ball is fixed to the bottom of the driven rod, and a second piston is arranged in the upper drip tube.

[0010] As a further solution of the present invention: the first piston is connected to a first spring, one end of which is connected to the top wall of the sleeve; the second piston is connected to a second spring, one end of which is connected to the top wall of the upper drip tube.

[0011] As a further solution of the present invention: a downward pressure rod is fixed to the bottom of the second piston, and the bottom of the downward pressure rod is a conical structure, connecting chambers are arranged on both sides of the lower drip tube, movable rods are movably arranged on the side walls of the two connecting chambers that are away from each other, and wedge blocks are fixed on the ends of the two movable rods that are close to each other, and a third spring is mounted on the movable rod, and the third spring is located between the wedge block and the connecting chamber.

[0012] As a further solution of the present invention: the driving mechanism includes a first motor and a second motor located in the loading platform, the output shaft of the first motor is connected to the first rotating shaft, the output shaft of the second motor is connected to the second rotating shaft, a gear is installed at one end of the second rotating shaft, a gear ring is mounted on the rotating frame, and the gear ring is meshed with the gear, and a plurality of sliding rods are fixed to the bottom of the rotating frame, and the bottom of the sliding rods is in contact with the surface of the loading platform.

[0013] As a further solution of the present invention: a notch is provided on the installation plate.

[0014] As a further solution of the present invention: a third rotating shaft is rotatably mounted at the bottom of the inner cavity of the lower drip tube, and a plurality of buffer blocks are arranged in an array along the circumferential direction on the third rotating shaft.

[0015] As a further solution of the present invention: the detection disc is fixed on the upper surface of the rotating frame, the bottom of the rotating frame is connected to the loading platform through a first rotating shaft, a plurality of support rods are fixed on the upper surface of the rotating frame, and a crest-shaped arc-shaped push block is fixed on the top of each support rod.

[0016] A method for using a SERS substrate-mounted detection device comprises the following steps:

[0017] S1. Place the prepared SERS substrate in a substrate holding tank, take appropriate amounts of the solutions to be added, and inject them into the lower dropping tube, and then fix the lower dropping tube to the upper dropping tube;

[0018] S2, using the driving mechanism to first drive the rotating frame to rotate, and the arc-shaped push block cooperates with the pneumatic transmission mechanism on one side of each upper dripping tube to automatically drip the solution into each substrate containing tank;

[0019] S3. The driving mechanism is then used to drive the detection disk to rotate, and the detection result can be obtained by using it in conjunction with the portable Raman instrument.

[0020] Beneficial effects of the present invention:

[0021] (1) The present invention sets a first rotating shaft connected to the loading platform, and the rotating frame is mounted outside the first rotating shaft and an arc-shaped push block is set, so that the automatic dripping of the solution can be realized through the cooperation of the pneumatic transmission mechanism and the arc-shaped push block, thereby improving the detection efficiency and accuracy. At the same time, the driving mechanism drives the first rotating shaft and the rotating frame to rotate respectively, thereby enhancing the convenience and automation of the experimental operation;

[0022] (2) The present invention provides a sleeve, a first piston, a driven rod, a driven ball, and a second piston. When the arc-shaped push block contacts the corresponding driven ball, the arc-shaped push block pushes the driven rod and the first piston to gradually rise, thereby using air pressure to push the second piston down, so that the solution drips from the lower drip tube into the substrate receiving tank, thereby improving the efficiency and accuracy of dripping.

[0023] (3) The present invention provides a connecting chamber, a wedge block, a lower pressure rod and a third spring. Initially, the two wedge blocks are in contact with each other, so that the solution will not drip randomly. When the lower pressure rod gradually contacts the two wedge blocks and separates the two wedge blocks, the solution will drip, thereby avoiding waste of the solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention will be further described below in conjunction with the accompanying drawings.

[0025] Figure 1 is a schematic diagram of the structure of the first viewing angle of the embodiment of the present invention;

[0026] Figure 2 is a schematic diagram of the structure of the second viewing angle of the first embodiment of the present invention;

[0027] Figure 3 is a schematic structural diagram of a loading platform according to a first embodiment of the present invention;

[0028] Figure 4 1 is a schematic structural diagram of a mounting plate, an upper dripping tube and a lower dripping tube according to a first embodiment of the present invention;

[0029] Figure 5 is a schematic structural diagram of an installation disk according to a first embodiment of the present invention;

[0030] Figure 6 Schematic diagram of the internal structure of the upper dripping tube, the lower dripping tube and the sleeve in the first embodiment of the present invention;

[0031] Figure 7 It is a structural diagram of embodiment 2 of the present invention.

[0032] In the figure: 1, loading platform; 2, first rotating shaft; 3, detection disc; 4, rotating frame; 5, sliding rod; 6, gear ring; 7, second rotating shaft; 8, gear; 9, fixing rod; 10, mounting plate; 11, supporting rod; 12, arc-shaped push block; 13, notch; 14, upper drip tube; 15, lower drip tube; 16, fixing block; 17, sleeve; 18, connecting pipe; 19, first spring; 20, first piston; 21, driven rod; 22, driven ball; 23, second spring; 24, second piston; 25, pressing rod; 26, connecting chamber; 27, movable rod; 28, wedge block; 29, third spring; 30, third rotating shaft; 31, buffer block.

[0033] The drawings are only used for illustrative purposes and are not to be construed as limitations of the present invention. To better illustrate the present embodiment, some parts of the drawings may be omitted, enlarged, or reduced in size, and do not represent the size and shape of actual products. For those skilled in the art, it is understandable that some well-known structures and their descriptions may be omitted in the drawings. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Example 1: Please refer to Figure 1-5As shown, the present invention is a SERS substrate carrying detection device, including a loading platform 1, a detection disc 3 and a rotating frame 4, a plurality of substrate accommodating grooves are provided on the detection disc 3, the detection disc 3 is connected to the loading platform 1 through a first rotating shaft 2, the rotating frame 4 is rotatably mounted on the outside of the first rotating shaft 2, a support rod 11 is provided on the upper surface of the rotating frame 4, a crest-shaped arc-shaped push block 12 is fixed on the top of the support rod 11, a mounting plate 10 is fixed on one side of the loading platform 1 through a fixing rod 9, a plurality of upper dripping tubes 14 are fixed on the mounting plate 10, a lower dripping tube 15 is detachably connected to the bottom end of the upper dripping tube 14, a pneumatic transmission mechanism is provided on one side of the upper dripping tube 14, and a pneumatic transmission mechanism is matched with the arc-shaped push block 12. The invention also includes a driving mechanism for driving the first rotating shaft 2 and the rotating frame 4 to rotate respectively; placing the prepared SERS substrate in the substrate containing tank, taking appropriate amounts of the solutions to be dripped, and then injecting them into the lower dripping tube 15, and then fixing the lower dripping tube 15 with the upper dripping tube 14. The lower dripping tube 15 and the upper dripping tube 14 can be fixed by means of threaded connection or bolt connection, and the specific connection method is not repeated here, and then the driving mechanism is used to drive the rotating frame 4 to rotate, driving the support rod 11 and the arc-shaped push block 12 to perform circular motion, and the arc-shaped push blocks 12 will cooperate with the pneumatic transmission mechanism one by one, so as to realize the automatic dripping of the solution and improve the detection efficiency and accuracy.

[0036] See also Figure 4 and Figure 6 The pneumatic transmission mechanism includes a sleeve 17 fixed to one side of the upper drip tube 14 through a fixing block 16, a connecting pipe 18 is connected between the sleeve 17 and the upper drip tube 14, a first piston 20 is arranged in the sleeve 17, a driven rod 21 is connected to the bottom of the first piston 20, a driven ball 22 is fixed to the bottom of the driven rod 21, a second piston 24 is arranged in the upper drip tube 14, a first spring 19 is connected to the first piston 20, one end of the first spring 19 is connected to the top wall of the sleeve 17, and the second A second spring 23 is connected to the piston 24, and one end of the second spring 23 is connected to the top wall of the upper drip tube 14; when the arc-shaped push block 12 contacts the corresponding driven ball 22, the arc-shaped push block 12 will push the driven rod 21 and the first piston 20 to gradually rise, thereby using air pressure to push the second piston 24 down, so that the solution drips from the lower drip tube 15 into the base receiving groove, and the first spring 19 and the second spring 23 automatically reset the first piston 20 and the second piston 24 to facilitate subsequent detection.

[0037] See also Figure 6A lower pressure rod 25 is fixed at the bottom of the second piston 24, and the bottom of the lower pressure rod 25 is a conical structure. A connecting chamber 26 is arranged on both sides of the lower dripping tube 15. A movable rod 27 is movably arranged on the side walls away from the two connecting chambers 26. A wedge block 28 is fixed at one end of the two movable rods 27 that are close to each other. A third spring 29 is sleeved on the movable rod 27, and the third spring 29 is located between the wedge block 28 and the connecting chamber 26; in order to avoid the waste caused by spontaneous dripping of the solution, two wedge blocks 28 are arranged in the lower dripping tube 15, and the solution is located above the two wedge blocks 28. When the second piston 24 descends, the lower pressure rod 25 descends synchronously. At this time, the upper dripping tube 14 and the lower dripping tube 15 will be pressurized first. After the lower pressure rod 25 contacts the two wedge blocks 28, the two wedge blocks 28 are automatically separated. At this time, the solution automatically falls, and due to the pressurization, the dripping speed of the solution can be accelerated, further improving the work efficiency.

[0038] See also Figure 1 , Figure 2 and Figure 3 The driving mechanism includes a first motor and a second motor located in the loading platform 1. The output shaft of the first motor is connected to the first rotating shaft 2, and the output shaft of the second motor is connected to the second rotating shaft 7. A gear 8 is installed at one end of the second rotating shaft 7. A ring gear 6 is mounted on the rotating frame 4, and the ring gear 6 is meshed with the gear 8. A plurality of sliding rods 5 are fixed to the bottom of the rotating frame 4, and the bottom of the sliding rods 5 is in contact with the surface of the loading platform 1.

[0039] See also Figure 5 A notch 13 is provided on the mounting plate 10, and the notch 13 is provided to facilitate the detection of the solution using a Raman instrument, thereby preventing the mounting plate 10 from obstructing the detection.

[0040] See also Figure 6 A third rotating shaft 30 is rotatably installed at the bottom of the inner cavity of the lower dripping tube 15, and a plurality of buffer blocks 31 are arranged in an array along the circumferential direction on the third rotating shaft 30; in order to prevent the solution from splashing to the outside due to excessive dripping speed, the buffer blocks 31 are arranged to buffer the solution, which speeds up the dripping rate while preventing the solution from splashing to the outside.

[0041] Example 2: In order to further speed up the dropping speed, in this example, refer to Figure 7As shown, the detection disc 3 is fixed on the upper surface of the rotating frame 4, the bottom of the rotating frame 4 is connected to the loading platform 1 through the first rotating shaft 2, and a plurality of support rods 11 are fixed on the upper surface of the rotating frame 4, and a crest-shaped arc-shaped push block 12 is fixed on the top of each support rod 11; in this embodiment, a plurality of arc-shaped push blocks 12 are arranged, and the detection disc 3 rotates together with the rotating frame 4, and when the arc-shaped push block 12 pushes the driven ball 22 to the highest point, the substrate receiving groove is just located under the lower dripping tube 15, and at this time, the lower pressure rod 25 separates the two wedge blocks 28 to enter the dripping of the solution, thereby avoiding the solution dripping to the outside of the substrate receiving groove and causing waste.

[0042] A method for using a SERS substrate-mounted detection device comprises the following steps:

[0043] S1, placing the prepared SERS substrate in a substrate holding tank, taking appropriate amounts of the solutions to be added, and then injecting them into the lower dropping tube 15, and then fixing the lower dropping tube 15 with the upper dropping tube 14;

[0044] S2, using the driving mechanism to first drive the rotating frame 4 to rotate, the arc-shaped push block 12 cooperates with the pneumatic transmission mechanism on one side of each upper dripping tube 14, so that the solution is automatically dripped into each substrate receiving groove;

[0045] S3, the driving mechanism is then used to drive the detection disk 3 to rotate, and the detection result can be obtained by using it in conjunction with the portable Raman instrument.

[0046] The working principle of the present invention is as follows: the prepared SERS substrate is placed in a plurality of substrate containing grooves, and appropriate amounts of the solution to be dripped are respectively injected into the lower dripping tube 15, and then the lower dripping tube 15 is fixedly connected with the upper dripping tube 14, and then the second motor drives the second rotating shaft 7 and the gear 8 to rotate, and the gear 8 drives the gear ring 6 and the rotating frame 4 to rotate, and drives the support rod 11 and the arc-shaped push block 12 to perform circular motion. When the arc-shaped push block 12 contacts the corresponding driven ball 22, the arc-shaped push block 12 will push the driven rod 21 and the first piston 20 to gradually rise, thereby using the air pressure to push the second piston 24 down. Due to the obstruction of the two wedge blocks 28, the solution will not drip immediately. When the arc-shaped push block 12 pushes the driven ball 22 to the highest point, the lower pressure rod 25 contacts the two wedge blocks 28, and the two wedge blocks 28 automatically separate. At this time, the solution automatically falls and is buffered by the buffer block 31. While accelerating the dripping rate, it also prevents the solution from splashing to the outside. After the solution is dripped, the first motor drives the first rotating shaft 2 to drive the detection disc 3 to rotate. When used in conjunction with the portable Raman instrument, a rotating sample measurement method is adopted, which is more conducive to the rapid detection and combination of the portable Raman instrument on site;

[0047] In order to further speed up the dripping efficiency, a plurality of arc-shaped push blocks 12 can be provided, and the detection disc 3 rotates with the rotating frame 4. When the arc-shaped push block 12 pushes the driven ball 22 to the highest point, the substrate receiving tank is just located directly below the lower dripping tube 15. At this time, the lower pressure rod 25 separates the two wedge blocks 28 to allow the solution to be dripped, thereby avoiding the solution from dripping to the outside of the substrate receiving tank and causing waste, and then the portable Raman instrument is used for detection.

[0048] The embodiments of the present invention are described in detail above, but the contents described are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made according to the scope of application of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A SERS substrate-mounted detection device, comprising a loading platform (1), a detection disc (3) and a rotating frame (4), wherein the detection disc (3) is provided with a plurality of substrate accommodating grooves, characterized in that: The detection disc (3) is connected to the loading platform (1) via a first rotating shaft (2); the rotating frame (4) is rotatably mounted on the outside of the first rotating shaft (2); a support rod (11) is arranged on the upper surface of the rotating frame (4); a wave-shaped arc-shaped push block (12) is fixed on the top of the support rod (11); a mounting plate (10) is fixed on one side of the loading platform (1) via a fixing rod (9); a plurality of upper dripping tubes (14) are fixed on the mounting plate (10); the bottom end of the upper dripping tube (14) is detachably connected to a lower dripping tube (15); a pneumatic transmission mechanism is arranged on one side of the upper dripping tube (14) to cooperate with the arc-shaped push block (12) to realize automatic dripping of the solution; It also comprises a driving mechanism for driving the first rotating shaft (2) and the rotating frame (4) to rotate respectively.

2. A SERS substrate-mounted detection device according to claim 1, characterized in that: The pneumatic transmission mechanism comprises a sleeve (17) fixed to one side of an upper dripping tube (14) through a fixing block (16); a connecting pipe (18) is connected between the sleeve (17) and the upper dripping tube (14); a first piston (20) is arranged in the sleeve (17); a driven rod (21) is connected to the bottom of the first piston (20); a driven ball (22) is fixed to the bottom of the driven rod (21); and a second piston (24) is arranged in the upper dripping tube (14).

3. A SERS substrate-mounted detection device according to claim 2, characterized in that: The first piston (20) is connected to a first spring (19), one end of which is connected to the top wall of the sleeve (17); the second piston (24) is connected to a second spring (23), one end of which is connected to the top wall of the upper drip tube (14).

4. A SERS substrate-mounted detection device according to claim 2, characterized in that: A pressing rod (25) is fixed at the bottom of the second piston (24), and the bottom of the pressing rod (25) is a conical structure. Communication chambers (26) are arranged on both sides of the lower drip tube (15). Movable rods (27) are movably arranged on the side walls of the two communication chambers (26) that are separated from each other. A wedge block (28) is fixed at the end of the two movable rods (27) that are close to each other. A third spring (29) is mounted on the movable rod (27), and the third spring (29) is located between the wedge block (28) and the communication chamber (26).

5. A SERS substrate-mounted detection device according to claim 1, characterized in that: The driving mechanism comprises a first motor and a second motor located inside the loading platform (1); the output shaft of the first motor is connected to the first rotating shaft (2); the output shaft of the second motor is connected to the second rotating shaft (7); a gear (8) is installed at one end of the second rotating shaft (7); a gear ring (6) is mounted on the rotating frame (4), and the gear ring (6) is meshed with the gear (8); a plurality of slide bars (5) are fixed to the bottom of the rotating frame (4), and the bottoms of the slide bars (5) are in contact with the surface of the loading platform (1).

6. A SERS substrate-mounted detection device according to claim 1, characterized in that: The installation plate (10) is provided with a notch (13).

7. A SERS substrate-mounted detection device according to claim 4, characterized in that: A third rotating shaft (30) is rotatably mounted at the bottom of the inner cavity of the lower dripping tube (15), and a plurality of buffer blocks (31) are arranged in an array along a circumferential direction on the third rotating shaft (30).

8. A SERS substrate-mounted detection device according to claim 1, characterized in that: The detection disc (3) is fixed on the upper surface of a rotating frame (4); the bottom of the rotating frame (4) is connected to the loading platform (1) via a first rotating shaft (2); a plurality of support rods (11) are fixed on the upper surface of the rotating frame (4); and a wave-shaped arc-shaped push block (12) is fixed on the top of each support rod (11).

9. The method for using a SERS substrate-mounted detection device according to claim 1, characterized in that: The following steps are involved: S1, placing the prepared SERS substrate in a substrate holding tank, taking appropriate amounts of the solutions to be added, and then injecting them into the lower dropping tube (15), and then fixing the lower dropping tube (15) and the upper dropping tube (14); S2, using the driving mechanism to first drive the rotating frame (4) to rotate, and the arc-shaped push block (12) cooperates with the air pressure transmission mechanism on one side of each upper dripping tube (14) to automatically drip the solution into each substrate containing groove; S3, the driving mechanism is then used to drive the detection disk (3) to rotate, and the detection result can be obtained by using it in conjunction with the portable Raman instrument.

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