Strain breeding preservation device for research and development of edible and medicinal fungi and preservation method thereof

Abstract

The invention belongs to the technical field of strain breeding preservation devices, and discloses a strain breeding preservation device for research and development of edible and medicinal fungi and a preservation method thereof.The strain breeding preservation device comprises a device body, a box door is hinged to one side of the device body, a storage rack is installed in the device body, and a ventilation assembly is arranged in the device body; the ventilation assembly comprises a fan frame arranged in the device body, a filtering assembly is arranged at the top end of the fan frame, a ventilation fan is installed in the fan frame, and a carbon dioxide monitor is installed on the side wall of the device body. Then the storage rack is placed in the device main body for cultivation, the carbon dioxide concentration in the device main body is monitored in real time through the carbon dioxide monitor during cultivation, and when the monitored carbon dioxide concentration reaches a certain threshold value, the ventilation fan is controlled to start ventilation through the PLC, so that the growth efficiency and quality are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of bacterial strain breeding and storage devices, and specifically relates to a bacterial strain breeding and storage device for the research and development of edible and medicinal fungi and a storage method thereof. Background Art

[0002] Edible and medicinal fungi are a large category of fungi that cover both edible and medicinal functions. They are on a par with the plant and animal kingdoms in nature, with extremely high biodiversity and important economic value. In the research and development of edible and medicinal fungi, strains are the foundation and core. In order to ensure the purity, activity and genetic stability of strains, strains must be properly preserved and managed; After searching, patent CN115627235A discloses a freeze-drying preservation method for Ganoderma lucidum mycelium, comprising: (1) inoculating Ganoderma lucidum seed liquid into a culture medium, culturing on a shaking table, adding sterilized polyvinyl pyrrolidone at a final concentration of 0.6% on the fifth day of culturing, and continuing culturing until the seventh day; (2) adding sterile skimmed milk powder at a final concentration of 10% to the Ganoderma lucidum seed liquid to be freeze-dried, standing in a refrigerator at 4°C overnight, adding sterile trehalose and mannitol to the Ganoderma lucidum seed liquid after standing overnight, and shaking well; (3) placing the Ganoderma lucidum seed liquid obtained in step (2) in a refrigerator at -20°C, and then transferring it to a freeze dryer for freeze-drying to obtain freeze-dried Ganoderma lucidum mycelium powder, freeze-drying the Ganoderma lucidum mycelium, and keeping the freeze-dried Ganoderma lucidum mycelium at room temperature for half a year without changing its seed activity; In the current process of breeding Ganoderma lucidum, the shaking table culture method is usually used. This culture method requires regular ventilation during the culture process to ensure that Ganoderma lucidum can grow in a suitable environment. Although the current shaking table equipment is equipped with a ventilation structure, the shaking table culture cycle is relatively long, and the Ganoderma lucidum strains have different requirements for ventilation time at different growth stages. The current ventilation structure is often difficult to adjust the ventilation frequency according to the growth stage of the Ganoderma lucidum strains, which to a certain extent affects the quality and efficiency of Ganoderma lucidum strain culture. Summary of the invention

[0003] The object of the present invention is to provide a strain breeding and storage device and a storage method for edible and medicinal fungi for research and development, so as to solve the problems raised in the above-mentioned background technology.

[0004] In order to achieve the above-mentioned object, the present invention provides the following technical solutions: a strain breeding and storage device for the research and development of edible and medicinal fungi, comprising a device body, a door is hingedly connected to one side of the device body, a storage rack is installed inside the device body, and a ventilation component is arranged inside the device body; Among them, the ventilation component includes a fan rack arranged inside the device body, a filter component is arranged on the top of the fan rack, a ventilation fan is installed inside the fan rack, first connecting rods are fixedly connected at the four corners of the fan rack, and mounting seats are arranged at the bottom ends of the four first connecting rods, each of the first connecting rods is connected to the mounting seat by a connecting bolt, and each of the mounting seats is fixedly installed inside the device body, a diversion hole and an air outlet are opened inside the device body, the diversion hole is arranged above the storage rack, and a carbon dioxide monitor is installed on the side wall of the device body.

[0005] As a further technical solution of the present invention, the filter assembly includes a filter plate arranged at the top of the fan frame, filter holes are evenly opened inside the filter plate, and a second connecting rod is fixedly connected at the four corners of the filter plate. The second connecting rod is arranged at the top of the first connecting rod, and a connecting assembly is arranged between the second connecting rod and the first connecting rod.

[0006] As a further technical solution of the present invention, a second filter is provided below the fan frame, and the second filter is installed inside the device body.

[0007] As a further technical solution of the present invention, a first filter is installed inside the air outlet.

[0008] As a further technical solution of the present invention, the connecting assembly includes a wedge embedded in the first connecting rod, one end of the wedge is fixedly connected to the first connecting plate, the first connecting plate is slidably installed in the second connecting rod, and a spring is provided on one side of the first connecting plate.

[0009] As a further technical solution of the present invention, the top end of the first connecting plate is fixedly connected to the second connecting plate, the top end of the second connecting plate is fixedly connected to the moving plate, and the moving plate is arranged on the top end of the filter plate.

[0010] As a further technical solution of the present invention, a connecting tube is embedded in the inner wall of the filter plate, a fixing rod is fixedly connected to the outer wall of the bottom end of the connecting tube, the fixing rod is fixedly installed on the inner wall of the filter hole, and a schematic tape is fixedly connected to the top end of the connecting tube.

[0011] As a further technical solution of the present invention, the schematic band is arranged on the top of the filter plate.

[0012] As a further technical solution of the present invention, an exhaust hole is provided at the top of the schematic tape.

[0013] A method for preserving a strain breeding storage device for the research and development of edible and medicinal fungi, comprising the following steps: S1: First, inoculate the Ganoderma lucidum seed liquid into the culture medium, place it on the top of the rack, and then place the rack in the main body of the device for cultivation. On the fifth day of cultivation, add sterilized polyvinyl pyrrolidone at a final concentration of 0.6%, and continue to cultivate until the seventh day; S2: Add sterile skimmed milk powder to the lyophilized Ganoderma lucidum seed liquid at a final concentration of 10%, and place it in a refrigerator at 4°C overnight. Add sterile trehalose and mannitol to the Ganoderma lucidum seed liquid after it has been placed overnight, and shake well. S3: placing the obtained Ganoderma lucidum seed liquid in a -20°C refrigerator, and then transferring it to a freeze dryer for freeze drying to obtain Ganoderma lucidum mycelium freeze-dried powder; Among them, S1 also includes: S11: During the culture process, the carbon dioxide concentration inside the device body is monitored in real time through a carbon dioxide monitor. When the monitored carbon dioxide concentration reaches a certain threshold, the ventilation fan is started through PLC control to allow external air to enter; S12: The external air is first filtered by the filter plate and the filter holes, then flows downward and contacts the second filter screen, is filtered again by the second filter screen, and is directed to the side wall of the device body through the diversion hole, thereby ventilating the inside of the device body; S13: When external air enters the filter hole, part of the gas enters the connecting pipe and flows upward to the schematic belt. The air pressure inside the connecting pipe and the schematic belt increases until the schematic belt is filled with gas, causing the schematic belt to expand and stretch, making it easier for staff to observe and maintain the filter plate.

[0014] The beneficial effects of the present invention are as follows: The present invention arranges a ventilation component. When cultivating ganoderma lucidum strains, ganoderma lucidum seed liquid is first inoculated into a culture medium and then placed on the top of a rack. The rack is then placed in a device body for cultivation. During cultivation, the carbon dioxide concentration inside the device body is monitored in real time through a carbon dioxide monitor. When the monitored carbon dioxide concentration reaches a certain threshold value that is preset, the ventilation fan is started through a PLC control to allow external air to enter and be discharged into the device body through a diversion hole for ventilation, so as to reduce the carbon dioxide concentration and provide a suitable and stable growth environment for the cultivation of ganoderma lucidum strains, thereby effectively improving its growth efficiency and quality.

[0015] The present invention sets a filter component. When the device starts the ventilation fan for ventilation, the external air is first filtered by the filter plate before entering the device. The gas filtering by the filter plate can reduce the possibility of external pollutants entering the device body as much as possible, avoid bacterial contamination, and improve the culture environment.

[0016] The present invention arranges the connection assembly. When installing the filter plate and the fan frame, the filter plate is first placed on the top of the fan frame, so that the second connecting rod is embedded in the inside of the first connecting rod. When the second connecting rod is embedded in the first connecting rod, the wedge block is embedded in the inside of the second connecting rod. Finally, the connecting bolt passes through the inner wall of the second connecting rod and the first connecting rod and is embedded in the mounting seat. Finally, the connecting bolt is rotated to install it in the inside of the mounting seat. During maintenance, the fan frame can be pulled upward together by the limiting of the wedge block, which is convenient for maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic cross-sectional view of the overall structure of the present invention; Figure 3 This is a schematic diagram of the structure of the mounting seat of the present invention; Figure 4 It is a structural schematic diagram of the filter plate of the present invention; Figure 5 It is a schematic diagram of the cross-section structure of the filter plate of the present invention; Figure 6 For the present invention Figure 5 A schematic diagram of the structure enlargement in the middle; Figure 7 It is a schematic diagram of the cross-section structure of the filter hole of the present invention; Figure 8 For the present invention Figure 7 A magnified schematic diagram of the structure at B in the middle; In the figure: 1. device body; 2. box door; 3. storage rack; 4. fan rack; 5. ventilation fan; 6. first connecting rod; 7. mounting base; 8. connecting bolt; 9. diversion hole; 10. carbon dioxide monitor; 11. air outlet; 12. first filter; 13. filter plate; 14. filter hole; 15. second connecting rod; 16. second filter; 17. first connecting plate; 18. wedge block; 19. spring; 20. second connecting plate; 21. movable plate; 22. connecting pipe; 23. fixing rod; 24. schematic tape; 25. exhaust hole. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] like Figures 1 to 8As shown, in an embodiment of the present invention, a strain breeding and storage device for edible and medicinal fungi research and development includes a device body 1, a door 2 is hingedly connected to one side of the device body 1, a storage rack 3 is installed inside the device body 1, and a ventilation component is arranged inside the device body 1; Among them, the ventilation component includes a fan frame 4 arranged inside the device body 1, a filter component is arranged on the top of the fan frame 4, a ventilation fan 5 is installed inside the fan frame 4, and first connecting rods 6 are fixedly connected at the four corners of the fan frame 4. The bottom ends of the four first connecting rods 6 are all provided with mounting seats 7. Each first connecting rod 6 is connected to the mounting seat 7 by a connecting bolt 8. Each mounting seat 7 is fixedly installed inside the device body 1. A diversion hole 9 and an air outlet 11 are opened inside the device body 1. The diversion hole 9 is arranged above the storage rack 3, and a carbon dioxide monitor 10 is installed on the side wall of the device body 1.

[0020] When cultivating the ganoderma lucidum, firstly, the ganoderma lucidum seed liquid is inoculated into the culture medium, and then placed on the top of the rack 3, and then the rack 3 is placed in the device body 1 for cultivation. During the cultivation, the carbon dioxide concentration inside the device body 1 is monitored in real time by the carbon dioxide monitor 10. When the monitored carbon dioxide concentration reaches a certain threshold value (the threshold value is preset), the ventilation fan 5 is started by the PLC control, so that the external air enters and is discharged into the device body 1 through the diversion hole 9 for ventilation, so as to reduce the carbon dioxide concentration, provide a suitable and stable growth environment for the cultivation of the ganoderma lucidum, and effectively improve its growth efficiency and quality; The four sides of the device body 1 are evenly provided with diversion holes 9, and the diversion holes 9 are arranged to be tilted downward, so that the gas is blown toward the side wall of the device body 1 when entering, so as to avoid the wind directly blowing toward the culture placed on the top of the rack 3, and prevent the mycelium from drying or being mechanically damaged, thereby ensuring the breeding quality; The mounting seat 7 is connected to the connecting bolt 8 by means of internal and external threads; The ventilating fan 5 is a common ventilation ventilating fan 5 structure, and its working method will not be described in detail here; The method for obtaining the ganoderma lucidum seed liquid is as follows: tissue expansion culture is performed on the original seed, and the expanded ganoderma lucidum seeds are inoculated into a liquid culture medium, placed in a 28°C constant temperature box, and cultured on a shaking table for 3 days at a temperature of 27°C and a rotation speed of 160 rpm / min; each 1000 ml of the liquid culture medium contains: 50 g of yeast extract powder, 3 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, 20 g of glucose, and 60 g of peptone; Each 1000 ml culture medium contains: 50 g yeast extract powder, 3 g potassium dihydrogen phosphate, 2 g magnesium sulfate, 20 g glucose, 60 g peptone, and 25 g sawdust.

[0021] like Figures 1 to 8As shown, the filter assembly includes a filter plate 13 arranged at the top of the fan frame 4, and filter holes 14 are evenly opened inside the filter plate 13. Second connecting rods 15 are fixedly connected at the four corners of the filter plate 13. The second connecting rod 15 is arranged at the top of the first connecting rod 6, and a connecting assembly is arranged between the second connecting rod 15 and the first connecting rod 6.

[0022] When the device starts the ventilation fan 5 for ventilation, the external air is first filtered by the filter plate 13 before entering the device. The gas filtering by the filter plate 13 can reduce the possibility of external pollutants entering the device body 1 as much as possible, avoid bacterial contamination, and improve the culture environment.

[0023] like Figure 3 As shown, a second filter screen 16 is provided below the fan frame 4 , and the second filter screen 16 is installed inside the device body 1 .

[0024] When the device is ventilated, the external air is first filtered by the filter plate 13 and then flows downward to contact the second filter screen 16, and is filtered again by the second filter screen 16 to further improve the culture environment inside the device body 1 to ensure the breeding quality of the strains; The second filter screen 16 is slidably connected to the mounting seat 7. When maintaining the second filter screen 16, the fan frame 4 and the filter plate 13 are first removed, and then the second filter screen 16 is directly taken out upward for maintenance.

[0025] like Figure 2 As shown, a first filter screen 12 is installed inside the air outlet 11 .

[0026] The possibility of external impurities entering the device through the air outlet 11 is reduced as much as possible.

[0027] like Figure 5 and Figure 6 As shown, the connecting assembly includes a wedge 18 embedded in the first connecting rod 6, one end of the wedge 18 is fixedly connected to the first connecting plate 17, the first connecting plate 17 is slidably installed in the second connecting rod 15, and a spring 19 is provided on one side of the first connecting plate 17.

[0028] When installing the filter plate 13 and the fan frame 4, the filter plate 13 is first placed on the top of the fan frame 4, so that the second connecting rod 15 is embedded in the first connecting rod 6. When the second connecting rod 15 is embedded in the first connecting rod 6, the inclined surface at the bottom end of the wedge block 18 contacts the outer wall of the first connecting rod 6 and slides into the second connecting rod 15 under force, and the spring 19 is deformed to store elastic potential energy; A sliding groove is provided inside the first connecting rod 6 for the wedge block 18 to slide. When the wedge block 18 moves to one side of the sliding groove, the spring 19 releases elastic potential energy to push the wedge block 18 to move outward and embed into the sliding groove, thereby connecting the second connecting rod 15 with the first connecting rod 6. Finally, the connecting bolt 8 passes through the inner wall of the second connecting rod 15 and the first connecting rod 6 and is embedded into the mounting seat 7. Finally, the connecting bolt 8 is rotated to install it inside the mounting seat 7. When the fan frame 4 needs to be maintained, the connecting bolts 8 are first rotated to remove them, and then the filter plate 13 is pulled upwards. The fan frame 4 can be pulled upwards together with the wedge block 18 to facilitate maintenance.

[0029] like Figure 6 As shown, the top end of the first connecting plate 17 is fixedly connected to the second connecting plate 20 , the top end of the second connecting plate 20 is fixedly connected to the moving plate 21 , and the moving plate 21 is arranged on the top end of the filter plate 13 .

[0030] When the filter plate 13 and the fan frame 4 need to be separated, the movable plate 21 is moved to one side, and the movement of the movable plate 21 drives the movement of the second connecting plate 20, and the movement of the second connecting plate 20 drives the movement of the first connecting plate 17, and the movement of the first connecting plate 17 drives the wedge block 18 to slide out of the first connecting rod 6, so as to facilitate the upward movement of the filter plate 13 and the separation from the fan frame 4.

[0031] like Figure 7 and Figure 8 As shown, a connecting tube 22 is embedded in the inner wall of the filter plate 13 , a fixing rod 23 is fixedly connected to the outer wall of the bottom end of the connecting tube 22 , the fixing rod 23 is fixedly installed on the inner wall of the filter hole 14 , and a schematic tape 24 is fixedly connected to the top end of the connecting tube 22 .

[0032] A connecting pipe 22 is installed inside some of the filter holes 14 through a fixing rod 23; The schematic band 24 is set to be an airtight material, such as plastic and rubber, and can expand after being inflated; Through the setting of the connecting pipe 22, during ventilation, air enters the interior of the filter hole 14, and part of the gas enters the interior of the connecting pipe 22 and flows upward to the schematic belt 24. The air pressure inside the connecting pipe 22 and the schematic belt 24 increases until the interior of the schematic belt 24 is filled with gas, causing the schematic belt 24 to expand and stretch. At this time, the staff can see that the schematic belt 24 has changed, and it is known that the gas inside the filter hole 14 is flowing, and the filter plate 13 does not need maintenance; As the use time increases, some impurities will accumulate inside the filter hole 14, causing blockage inside the filter hole 14 or poor gas circulation. At this time, the air pressure inside the connecting tube 22 is insufficient, and the signal belt 24 cannot be stretched or the expansion effect is insufficient. The staff can observe that the filter plate 13 needs to be maintained, which facilitates timely maintenance of the filter plate 13 and ensures a sterile environment inside the device body 1 to breed the strains, thereby improving the strain breeding quality during the research and development of edible and medicinal fungi.

[0033] like Figure 7 As shown, the schematic band 24 is arranged on the top of the filter plate 13.

[0034] like Figure 8 As shown, a vent hole 25 is provided at the top of the belt 24 .

[0035] During ventilation, gas always enters the interior of the schematic tape 24 , and part of the gas can be discharged through the exhaust holes 25 to ensure the stability and reliability of the schematic tape 24 .

[0036] A method for preserving a strain breeding storage device for the research and development of edible and medicinal fungi, comprising the following steps: S1: First, inoculate the Ganoderma lucidum seed liquid into the culture medium, place it on the top of the rack 3, and then place the rack 3 in the device body 1 for cultivation. On the fifth day of cultivation, add sterilized polyvinyl pyrrolidone at a final concentration of 0.6%, and continue to cultivate until the seventh day to end the cultivation; S2: Add sterile skimmed milk powder to the lyophilized Ganoderma lucidum seed liquid at a final concentration of 10%, and place it in a refrigerator at 4°C overnight. Add sterile trehalose and mannitol to the Ganoderma lucidum seed liquid after it has been placed overnight, and shake well. S3: placing the obtained Ganoderma lucidum seed liquid in a -20°C refrigerator, and then transferring it to a freeze dryer for freeze drying to obtain Ganoderma lucidum mycelium freeze-dried powder; Among them, S1 also includes: S11: During the culture process, the carbon dioxide concentration inside the device body 1 is monitored in real time by the carbon dioxide monitor 10. When the monitored carbon dioxide concentration reaches a certain threshold, the ventilation fan 5 is started by the PLC control to allow external air to enter; S12: The external air is first filtered by the filter plate 13 and the filter hole 14, then flows downward and contacts the second filter screen 16, is filtered again by the second filter screen 16, and is directed to the side wall of the device body 1 through the diversion hole 9, thereby ventilating the inside of the device body 1; S13: When external air enters the filter hole 14, part of the gas enters the connecting pipe 22 and flows upward to the schematic belt 24. The air pressure inside the connecting pipe 22 and the schematic belt 24 increases until the schematic belt 24 is filled with gas, causing the schematic belt 24 to expand and stretch, making it easier for staff to observe and maintain the filter plate 13.

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for preserving strains of edible and medicinal fungi for research and development, comprising a device body (1), characterized in that: A door (2) is hingedly connected to one side of the device body (1), a storage rack (3) is installed inside the device body (1), and a ventilation component is arranged inside the device body (1); The ventilation assembly comprises a fan frame (4) arranged inside the device body (1), a filter assembly is arranged at the top of the fan frame (4), a ventilation fan (5) is installed inside the fan frame (4), four corners of the fan frame (4) are fixedly connected with first connecting rods (6), the bottom ends of the four first connecting rods (6) are provided with mounting seats (7), each of the first connecting rods (6) is connected to the mounting seat (7) by a connecting bolt (8), each of the mounting seats (7) is fixedly installed inside the device body (1), a diversion hole (9) and an air outlet hole (11) are opened inside the device body (1), the diversion hole (9) is arranged above the storage rack (3), and a carbon dioxide monitor (10) is installed on the side wall of the device body (1).

2. The device for storing and breeding edible and medicinal fungi according to claim 1, characterized in that: The filter assembly comprises a filter plate (13) arranged at the top end of the fan frame (4), filter holes (14) are evenly arranged inside the filter plate (13), second connecting rods (15) are fixedly connected at four corners of the filter plate (13), the second connecting rod (15) is arranged at the top end of the first connecting rod (6), and a connecting assembly is arranged between the second connecting rod (15) and the first connecting rod (6).

3. The device for breeding and preserving edible and medicinal fungi according to claim 1, characterized in that: A second filter (16) is provided below the fan frame (4), and the second filter (16) is installed inside the device body (1).

4. The device for storing and breeding edible and medicinal fungi according to claim 1, characterized in that: A first filter screen (12) is installed inside the air outlet hole (11).

5. The device for storing and breeding edible and medicinal fungi according to claim 2, characterized in that: The connecting assembly comprises a wedge (18) embedded in the first connecting rod (6), one end of the wedge (18) is fixedly connected to a first connecting plate (17), the first connecting plate (17) is slidably mounted inside the second connecting rod (15), and a spring (19) is provided on one side of the first connecting plate (17).

6. The device for storing and breeding edible and medicinal fungi according to claim 5, characterized in that: The top end of the first connecting plate (17) is fixedly connected to a second connecting plate (20), the top end of the second connecting plate (20) is fixedly connected to a movable plate (21), and the movable plate (21) is arranged on the top end of the filter plate (13).

7. The device for storing and breeding edible and medicinal fungi according to claim 2, characterized in that: A connecting tube (22) is embedded in the inner wall of the filter plate (13); a fixing rod (23) is fixedly connected to the outer wall of the bottom end of the connecting tube (22); the fixing rod (23) is fixedly mounted on the inner wall of the filter hole (14); and a schematic tape (24) is fixedly connected to the top end of the connecting tube (22).

8. The device for storing and breeding edible and medicinal fungi according to claim 7, characterized in that: The schematic band (24) is arranged on the top of the filter plate (13).

9. A bacterial strain breeding and storage device for research and development of edible and medicinal fungi according to claim 8, characterized in that: An exhaust hole (25) is provided at the top end of the schematic band (24).

10. A method for preserving a strain breeding storage device for edible and medicinal fungi research and development, the method being applicable to the strain breeding storage device for edible and medicinal fungi research and development as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: S1: First, inoculate the Ganoderma lucidum seed liquid into the culture medium, place it on the top of the rack (3), then place the rack (3) in the device body (1) for culture, add sterilized polyvinyl pyrrolidone at a final concentration of 0.6% on the fifth day of culture, and continue to culture until the seventh day to end the culture; S2: Add sterile skimmed milk powder to the lyophilized Ganoderma lucidum seed liquid at a final concentration of 10%, and place it in a refrigerator at 4°C overnight. Add sterile trehalose and mannitol to the Ganoderma lucidum seed liquid after it has been placed overnight, and shake well. S3: placing the obtained Ganoderma lucidum seed liquid in a -20°C refrigerator, and then transferring it to a freeze dryer for freeze drying to obtain Ganoderma lucidum mycelium freeze-dried powder; Among them, S1 also includes: S11: During the culture process, the carbon dioxide concentration inside the device body (1) is monitored in real time by a carbon dioxide monitor (10). When the monitored carbon dioxide concentration reaches a certain threshold, the ventilation fan (5) is started by PLC control to allow external air to enter; S12: the external air is first filtered by the filter plate (13) and the filter holes (14), then flows downward and contacts the second filter screen (16), is filtered again by the second filter screen (16), and is directed through the diversion holes (9) to be sprayed onto the side wall of the device body (1), thereby ventilating the inside of the device body (1); S13: When external air enters the filter hole (14), part of the gas enters the interior of the connecting pipe (22) and flows upward to the schematic band (24). The internal air pressure of the connecting pipe (22) and the schematic band (24) increases until the interior of the schematic band (24) is filled with gas, causing the schematic band (24) to expand and stretch, thereby facilitating the staff to observe and maintain the filter plate (13).

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