Method for preventing and controlling mycoplasma synoviae

Abstract

The invention relates to the technical field of poultry rearing, in particular to a mycoplasma synoviae prevention and control method which comprises the following steps: managing provenance, selecting a qualified breeding chicken house to introduce laying hens, and disinfecting and purifying the breeding chicken house to reduce vertical propagation of mycoplasma; nutrition management: providing a nutrition formula, and supporting healthy development of the laying hens in each growth stage; drug prevention and control, wherein drug prevention and control measures are made according to the pathogenesis law of the mycoplasma; and feeding management: implementing feeding management measures. By managing and adjusting mycoplasma infection, chicken flock health control is effectively improved. Through regular purification measures, especially implementation in the key growth period, the long-term infection of mycoplasma in the chicken body is effectively prevented, and the infection risk of the fallopian tube is reduced. The scientifically adjusted nutrition formula ensures that the chicken can obtain sufficient nutrition in each growth stage, healthy growth is promoted, immunity is enhanced, and occurrence of diseases is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of poultry breeding, and in particular to a method for preventing and controlling Mycoplasma synoviae. Background Art

[0002] Synovial mycoplasma disease is an infectious disease of chickens caused by Mycoplasma Synoviae (MS). The disease is characterized by swelling of joints, tendon sheaths and soles, dry cool substances in the air sacs, "capped eggs", sandy shell eggs, and broken shell eggs in laying hens, accompanied by a decrease in egg production. MS is a Gram-negative bacterium without cell walls. It is resistant to antibiotics that can affect cell wall synthesis. MS is unstable at pH 6.8 or lower and is sensitive to temperatures above 39°C. Mycoplasma on feathers can survive for up to 3 days, but not more than 1 day on other materials. The morphology is different when observed under an electron microscope. Some are round, some are filamentous, there is only one serotype, and the difference between different strains is very small.

[0003] However, existing breeding methods have limitations in the management of mycoplasma infection. Due to the lack of careful management of seed sources and breeding environments, chickens are often faced with disease threats without adequate protection. Conventional methods do not fully consider the specific needs of chickens at different growth stages in nutrition and drug prevention and control, resulting in poor overall health and production performance of the flock. Disease prevention and control fails to conduct effective monitoring and intervention in a timely manner, and the disease spreads among the flock, affecting the entire egg-laying cycle. These deficiencies lead to reduced production efficiency, increased breeding costs, and affected egg quality. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for preventing and controlling Mycoplasma synoviae.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme, a method for preventing and controlling Mycoplasma synoviae, comprising the following steps:

[0006] Seed source management: select qualified breeder farms to introduce laying hens, and disinfect and purify the breeder farms to reduce the vertical transmission of mycoplasma;

[0007] Nutritional management, providing nutritional formulas to support the healthy development of laying hens at every growth stage;

[0008] Drug prevention and control: formulate drug prevention and control measures according to the pathogenesis of mycoplasma;

[0009] Feeding management, implementation of feeding management measures;

[0010] Liver function care, implement health care measures to support liver health.

[0011] Preferably, the breeder farm should have formal purification procedures and qualifications.

[0012] Preferably, the feeding and management measures are specifically:

[0013] Data recording of feed intake, water intake and egg production rate;

[0014] Maintain the uniformity of laying hens’ weight not less than 80%;

[0015] Manage the temperature difference in the laying hen house within 5°C to avoid immunosuppression and mycoplasma infection.

[0016] Preferably, the drug prevention and control measures are specifically:

[0017] Mycoplasma purification was performed at 14 and 50 days of age according to the incubation period of mycoplasma infection;

[0018] After the transfer stress, mycoplasma purification should be performed immediately to prevent infection;

[0019] Mycoplasma purification should be carried out 1-5 days before the chickens are 90 days old to prevent mycoplasma infection in the oviducts of laying hens.

[0020] Preferably, the mycoplasma purification is specifically performed using enrofloxacin, and the treatment cycle is 7 days.

[0021] Preferably, the nutritional formula comprises by weight:

[0022] Formula for brooding period of 0-6 weeks: crude protein: 280-320 parts, main ingredients: 2800-3200 parts, calcium: 7-10 parts, phosphorus: 6-8 parts, vitamin A: 10-15 parts, vitamin D3: 4-6 parts;

[0023] 7-20 weeks growth period formula: crude protein: 250-290 parts, main ingredients: 2700-3100 parts, calcium: 8-12 parts, phosphorus: 7-9 parts, vitamin A: 9-14 parts, vitamin D3: 3-5 parts;

[0024] Formula for the first-time mothers at 21 weeks: crude protein: 270-310 parts, main ingredients: 2750-3150 parts, calcium: 10-13 parts, phosphorus: 8-11 parts, vitamin A: 11-16 parts, vitamin D3: 4-7 parts;

[0025] Peak production formula after 25 weeks: crude protein: 260-300 parts, main ingredients: 2800-3200 parts, calcium: 11-14 parts, phosphorus: 9-12 parts, vitamin A: 12-17 parts, vitamin D3: 5-8 parts;

[0026] The main ingredient is a mixture of corn and wheat, and the mixing ratio is 1:1.

[0027] Preferably, the health care measures specifically include the following steps:

[0028] S1, assess health status: use a fully digital ultrasonic diagnostic instrument to perform morphological and functional examinations on the liver of the chickens; at the same time, draw 2mL of venous blood samples and use an automated blood analyzer to test white blood cell counts and liver enzyme activity; summarize and form an initial health assessment report;

[0029] S2, select health care agent: select antimicrobial peptide princepsin as health care agent;

[0030] S3, develop a feeding plan: If there are liver problems, feed 0.75g of health supplement twice a day for 30 days;

[0031] S4: Repeat step S1 every week to obtain a detailed health assessment report and compare it with the initial health assessment report to evaluate the degree of improvement in liver function. If there is no improvement within one month, contact a veterinarian for treatment.

[0032] Compared with the prior art, the advantages and positive effects of the present invention are:

[0033] The present invention effectively improves the health control of chicken flocks by managing and adjusting mycoplasma infection. Regular purification measures, especially those implemented during the critical growth period, effectively prevent long-term infection of mycoplasma in chickens and reduce the risk of oviduct infection. The scientifically adjusted nutritional formula ensures that chickens obtain adequate nutrition at each growth stage, promotes healthy growth, enhances immunity, and reduces the occurrence of diseases. Continuous monitoring of liver function and health care measures enhance the liver's detoxification and metabolic functions, allowing chickens to better respond to disease challenges. These measures work together to achieve effective disease prevention and control, improve egg production and egg quality, and enhance economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the steps of the present invention. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] Example 1, please refer to Figure 1 The present invention provides a technical solution, a method for preventing and controlling Mycoplasma synoviae, comprising the following steps:

[0037] Seed source management: select qualified breeder farms to introduce laying hens, and disinfect and purify the breeder farms to reduce the vertical transmission of mycoplasma;

[0038] Nutritional management, providing nutritional formulas to support the healthy development of laying hens at every growth stage;

[0039] Drug prevention and control: formulate drug prevention and control measures according to the pathogenesis of mycoplasma;

[0040] Feeding management, implementation of feeding management measures;

[0041] Liver function care, implement health care measures to support liver health.

[0042] In this embodiment, the breeder farm should have formal purification procedures and qualifications.

[0043] In this embodiment, the feeding and management measures are specifically:

[0044] Data recording of feed intake, water intake and egg production rate;

[0045] Maintain the uniformity of laying hens’ weight not less than 80%;

[0046] Manage the temperature difference in the laying hen house within 5°C to avoid immunosuppression and mycoplasma infection.

[0047] In this embodiment, the drug prevention and control measures are specifically:

[0048] Mycoplasma purification was performed at 14 and 50 days of age according to the incubation period of mycoplasma infection;

[0049] After the transfer stress, mycoplasma purification should be performed immediately to prevent infection;

[0050] Mycoplasma purification should be carried out 5 days before the chickens are 90 days old to prevent mycoplasma infection in the oviducts of laying hens.

[0051] In this embodiment, the mycoplasma purification is specifically performed using enrofloxacin, and the treatment cycle is 7 days.

[0052] In this embodiment, the nutritional formula includes, by weight:

[0053] Formula for brooding period of 0-6 weeks: crude protein: 320 parts, main ingredients: 3200 parts, calcium: 10 parts, phosphorus: 8 parts, vitamin A: 15 parts, vitamin D3: 6 parts;

[0054] 7-20 weeks growth period formula: crude protein: 290 parts, main ingredients: 3100 parts, calcium: 12 parts, phosphorus: 9 parts, vitamin A: 14 parts, vitamin D3: 5 parts;

[0055] Formula for the first-born period at 21 weeks: crude protein: 310 parts, main ingredients: 3150 parts, calcium: 13 parts, phosphorus: 11 parts, vitamin A: 16 parts, vitamin D3: 7 parts;

[0056] Peak production formula after 25 weeks: crude protein: 300 parts, main ingredients: 3200 parts, calcium: 14 parts, phosphorus: 12 parts, vitamin A: 17 parts, vitamin D3: 8 parts;

[0057] The main ingredient is a mixture of corn and wheat, and the mixing ratio is 1:1.

[0058] In this embodiment, the health care measures specifically include the following steps:

[0059] S1, assess health status: use a fully digital ultrasonic diagnostic instrument to perform morphological and functional examinations on the liver of the chickens; at the same time, draw 2mL of venous blood samples and use an automated blood analyzer to test white blood cell counts and liver enzyme activity; summarize and form an initial health assessment report;

[0060] S2, select health care agent: select antimicrobial peptide princepsin as health care agent;

[0061] S3, develop a feeding plan: If there are liver problems, feed 0.75g of health supplement twice a day for 30 days;

[0062] S4: Repeat step S1 every week to obtain a detailed health assessment report and compare it with the initial health assessment report to evaluate the degree of improvement in liver function. If there is no improvement within one month, contact a veterinarian for treatment.

[0063] Specifically, in the health assessment step, a fully digital ultrasound diagnostic instrument is used to perform morphological and functional examinations on the liver of the chickens. This process involves high-precision imaging technology to ensure that the structure and functional status of the liver can be observed in detail. The fully digital ultrasound diagnostic instrument emits high-frequency sound waves, which are reflected back when encountering the tissue interface, received by the instrument and converted into images. This technology can not only observe the morphology of the liver, such as size, shape and edge characteristics, but also evaluate blood flow conditions, such as checking the blood flow velocity and direction in the blood vessels through the Doppler function, so as to comprehensively evaluate liver function. At the same time, the operator draws 2mL of venous blood samples and uses an automated blood analyzer to count white blood cells and detect liver enzyme activity. This process is highly automated, ensuring the accuracy and repeatability of the results. The automated blood analyzer classifies and counts blood cells through optical and electronic technologies, and evaluates liver enzyme levels through biochemical analysis technology, thus providing an important basis for disease diagnosis. Through the comprehensive analysis of these data, an initial health assessment report is formed. This report records the test data and analysis results in detail, providing a scientific basis for subsequent health care and treatment.

[0064] In the step of selecting health care agents, after comprehensive evaluation and comparison of the effects of different health care agents, the antimicrobial peptide princeps was selected as a health care agent. Antimicrobial peptides are a class of small molecule proteins with broad antimicrobial activity, which can effectively inhibit the growth of a variety of bacteria and viruses and enhance the immunity of animals. As an effective antimicrobial peptide, princeps helps improve the health of animals by blocking the synthesis of bacterial cell walls, leading to bacterial death. This health care agent was selected based on its high safety, low side effects, and no negative impact on animal growth and development. It aims to prevent diseases and improve production efficiency by strengthening the immune system.

[0065] In the step of formulating a feeding plan, based on the results of the liver examination, if liver problems are found, a specific feeding plan is formulated to feed 0.75g of health care agent twice a day for 30 consecutive days. The formulation of the feeding plan is based on accurately measured health care doses and continuous treatment cycles, aiming to promote the recovery of liver function and improvement of overall health through continuous and quantitative health care agent input. This feeding plan is formulated under the guidance of veterinarians and nutritionists based on the specific health status and weight of the animal to ensure that the effect of the health care agent is maximized and does not have a negative impact on the animal.

[0066] In the step of repeating the health assessment every week, a detailed health assessment report is obtained by repeating the same procedures of the initial health assessment. The purpose of this operation is to monitor changes in liver function and overall health status to evaluate the degree of improvement in liver function. By comparing the new health assessment report each week with the initial report, the effectiveness of health care measures can be accurately tracked, and feeding and health care strategies can be adjusted in a timely manner. If the assessment results for more than one month in a row show that liver function has not improved as expected, it will be recommended to contact a veterinarian for more in-depth diagnosis and treatment. This step is a dynamic adjustment process that ensures that each assessment accurately reflects the current health status of the animal and provides a basis for further treatment measures.

[0067] Example 2, please refer to Figure 1 The present invention provides a technical solution, a method for preventing and controlling Mycoplasma synoviae, comprising the following steps:

[0068] Seed source management: select qualified breeder farms to introduce laying hens, and disinfect and purify the breeder farms to reduce the vertical transmission of mycoplasma;

[0069] Nutritional management, providing nutritional formulas to support the healthy development of laying hens at every growth stage;

[0070] Drug prevention and control: formulate drug prevention and control measures according to the pathogenesis of mycoplasma;

[0071] Feeding management, implementation of feeding management measures;

[0072] Liver function care, implement health care measures to support liver health.

[0073] In this embodiment, the breeder farm should have formal purification procedures and qualifications.

[0074] In this embodiment, the feeding and management measures are specifically:

[0075] Data recording of feed intake, water intake and egg production rate;

[0076] Maintain the uniformity of laying hens’ weight not less than 80%;

[0077] Manage the temperature difference in the laying hen house within 5°C to avoid immunosuppression and mycoplasma infection.

[0078] In this embodiment, the drug prevention and control measures are specifically:

[0079] Mycoplasma purification was performed at 14 and 50 days of age according to the incubation period of mycoplasma infection;

[0080] After the transfer stress, mycoplasma purification should be performed immediately to prevent infection;

[0081] Mycoplasma purification should be carried out 3 days before the chickens are 90 days old to prevent mycoplasma infection in the oviducts of laying hens.

[0082] In this embodiment, the mycoplasma purification is specifically performed using enrofloxacin, and the treatment cycle is 7 days.

[0083] In this embodiment, the nutritional formula includes, by weight:

[0084] Formula for brooding period of 0-6 weeks: crude protein: 280 parts, main ingredients: 2800 parts, calcium: 10 parts, phosphorus: 6 parts, vitamin A: 15 parts, vitamin D3: 4 parts;

[0085] 7-20 weeks growth period formula: crude protein: 290 parts, main ingredients: 3100 parts, calcium: 8 parts, phosphorus: 9 parts, vitamin A: 14 parts, vitamin D3: 4 parts;

[0086] Formula for the first-born period at 21 weeks: crude protein: 310 parts, main ingredients: 3150 parts, calcium: 10 parts, phosphorus: 11 parts, vitamin A: 11 parts, vitamin D3: 4 parts;

[0087] Peak production formula after 25 weeks: crude protein: 300 parts, main ingredients: 2800 parts, calcium: 11 parts, phosphorus: 9 parts, vitamin A: 12 parts, vitamin D3: 5 parts;

[0088] The main ingredient is a mixture of corn and wheat, and the mixing ratio is 1:1.

[0089] In this embodiment, the health care measures specifically include the following steps:

[0090] S1, assess health status: use a fully digital ultrasonic diagnostic instrument to perform morphological and functional examinations on the liver of the chickens; at the same time, draw 2mL of venous blood samples and use an automated blood analyzer to test white blood cell counts and liver enzyme activity; summarize and form an initial health assessment report;

[0091] S2, select health care agent: select antimicrobial peptide princepsin as health care agent;

[0092] S3, develop a feeding plan: If there are liver problems, feed 0.75g of health supplement twice a day for 30 days;

[0093] S4: Repeat step S1 every week to obtain a detailed health assessment report and compare it with the initial health assessment report to evaluate the degree of improvement in liver function. If there is no improvement within one month, contact a veterinarian for treatment.

[0094] Experimental methods:

[0095] Infection rate experiment: The swab samples of chicken throat were tested by real-time quantitative PCR, and then rinsed with sterile saline and stored. Specific primers and probes were used to amplify and detect the target mycoplasma DNA, and the number of positive samples was recorded and compared with the total number of samples to calculate the infection rate. The amplification conditions were pre-denaturation at 95°C for 10 minutes, followed by 40 cycles (95°C for 15 seconds, 60°C for 60 seconds).

[0096] Average egg production rate: Record the number of eggs produced daily for 90 days through continuous observation and recording. Count the number of eggs produced by each chicken every day, and calculate the ratio of the total number of eggs produced to the number of chickens to get the average egg production rate. Ensure that the temperature, humidity, light and other factors of the breeding environment are kept standardized.

[0097] Average weight gain: Weigh each bird at the beginning and end of the experiment. Calculate the weight gain of each bird and find the average weight gain of all birds. Keep the conditions consistent, ensuring that feed and water are adequate and standardized.

[0098] Mortality rate: Record the number of dead chickens over a certain period (e.g. 90 days) through continuous monitoring and recording. Calculate the mortality rate by dividing the number of dead chickens by the total number of chickens. All chickens should be raised under the same environmental conditions to ensure the accuracy of the data.

[0099] Experiments were conducted on Examples 1-2, wherein Comparative Example 1 was a traditional chicken farming process, and the experimental results were as follows:

[0100] Table 1 Experimental results

[0101] Performance Indicators Comparative Example 1 Example 1 Example 2 Infection rate (%) 15 5 4 Average egg production rate (%) 80 88 85 Average weight gain (%) 5 8 7 mortality rate(%) 12 4 3

[0102] As can be seen from Table 1, Example 1-2 has significant advantages over Comparative Example 1 in multiple key performance indicators. Specifically, Example 1-2 performs better in reducing the infection rate, can effectively control the spread of the disease, and enhance the health level of the chickens. In terms of improving the average egg production rate, Example 1-2 significantly improves the production efficiency of laying hens by optimizing nutrition and management measures. In addition, Example 1-2 also optimizes the weight gain of chickens, which helps to improve the overall growth rate and meat quality of the chickens. Finally, Example 1-2 reduces the mortality rate, improves the survival rate of chickens, and further ensures the health and economic benefits of the flock.

[0103] The above are only preferred embodiments of the present invention and are not intended to limit the present invention in other forms. Any technician familiar with the profession may use the technical contents disclosed above to change or modify them into equivalent embodiments with equivalent changes and apply them to other fields. However, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A method for preventing and controlling Mycoplasma synoviae, characterized in that: The following steps are involved: Seed source management: select qualified breeder farms to introduce laying hens, and disinfect and purify the breeder farms to reduce the vertical transmission of mycoplasma; Nutritional management, providing nutritional formulas to support the healthy development of laying hens at every growth stage; Drug prevention and control: formulate drug prevention and control measures according to the pathogenesis of mycoplasma; Feeding management, implementation of feeding management measures; Liver function care, implement health care measures to support liver health.

2. The method for preventing and controlling Mycoplasma synoviae according to claim 1, characterized in that: The breeder farm should have formal purification procedures and qualifications.

3. The method for preventing and controlling Mycoplasma synoviae according to claim 1, characterized in that: The feeding and management measures are specifically: Data recording of feed intake, water intake and egg production rate; Maintain the uniformity of laying hens’ weight not less than 80%; Manage the temperature difference in the laying hen house within 5°C to avoid immunosuppression and mycoplasma infection.

4. The method for preventing and controlling Mycoplasma synoviae according to claim 1, characterized in that: The drug prevention and control measures are specifically: Mycoplasma purification was performed at 14 and 50 days of age according to the incubation period of mycoplasma infection; After the transfer stress, mycoplasma purification should be performed immediately to prevent infection; Mycoplasma purification should be carried out 1-5 days before the chickens are 90 days old to prevent mycoplasma infection in the oviducts of laying hens.

5. The method for preventing and controlling Mycoplasma synoviae according to claim 4, characterized in that: The mycoplasma purification is specifically performed using enrofloxacin, and the treatment cycle is 7 days.

6. The method for preventing and controlling Mycoplasma synoviae according to claim 1, characterized in that: The nutritional formula comprises by weight: Formula for brooding period of 0-6 weeks: crude protein: 280-320 parts, main ingredients: 2800-3200 parts, calcium: 7-10 parts, phosphorus: 6-8 parts, vitamin A: 10-15 parts, vitamin D3: 4-6 parts; 7-20 weeks growth period formula: crude protein: 250-290 parts, main ingredients: 2700-3100 parts, calcium: 8-12 parts, phosphorus: 7-9 parts, vitamin A: 9-14 parts, vitamin D3: 3-5 parts; Formula for the first-time mothers at 21 weeks: crude protein: 270-310 parts, main ingredients: 2750-3150 parts, calcium: 10-13 parts, phosphorus: 8-11 parts, vitamin A: 11-16 parts, vitamin D3: 4-7 parts; Peak production formula after 25 weeks: crude protein: 260-300 parts, main ingredients: 2800-3200 parts, calcium: 11-14 parts, phosphorus: 9-12 parts, vitamin A: 12-17 parts, vitamin D3: 5-8 parts; The main ingredient is a mixture of corn and wheat, and the mixing ratio is 1:

1.

7. The method for preventing and controlling Mycoplasma synoviae according to claim 1, characterized in that: The health care measures specifically include the following steps: S1, assess health status: use a fully digital ultrasonic diagnostic instrument to perform morphological and functional examinations on the liver of the chickens; at the same time, draw 2mL of venous blood samples and use an automated blood analyzer to test white blood cell counts and liver enzyme activity; summarize and form an initial health assessment report; S2, select health care agent: select antimicrobial peptide princepsin as health care agent; S3, develop a feeding plan: If there is a liver problem, feed twice a day, each time 0.75g of health supplement for 30 consecutive days; S4: Repeat step S1 every week to obtain a detailed health assessment report and compare it with the initial health assessment report to evaluate the degree of improvement in liver function. If there is no improvement within one month, contact a veterinarian for treatment.

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