Method for extracting total polyphenol from peach branch medicinal material

Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for extracting total polyphenol from a peach branch medicinal material. According to the method, an ethanol solution is adopted as an extraction solvent to carry out ultrasonic extraction on the total polyphenol in the peach branch medicinal material. By investigating the extraction process, the optimal extraction process conditions of the total polyphenol of the peach branch medicinal material are as follows: the extraction solvent is ethanol, the extraction mode is ultrasonic extraction, the extraction time is 80min, the material-liquid ratio is 1: 40g / mL, the volume fraction of ethanol is 60%, and the yield of the total polyphenol of the peach branch medicinal material is higher under the conditions. The extraction process is simple and efficient, the total polyphenol yield is high, and powerful support is provided for quality evaluation and medicinal material development and utilization of peach branch medicinal materials.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine extraction, and in particular to a method for extracting total polyphenols from peach branch medicinal materials. Background Art

[0002] Peach branch is the dried branch of Prunus persica (L.) Batsch, a plant of the Rosaceae family. Peach branch tastes bitter and flat, and enters the heart and liver meridians. It is used to treat heart and abdominal pain, rheumatism, traumatic injuries, sores, etc. It has the effects of promoting blood circulation, unblocking meridians, detoxifying and killing insects. [1] The chemical components contained in peach branch medicinal materials mainly include phenolic compounds, flavonoid compounds, sugars, etc. Studies have shown that total polyphenols have good antioxidant effects and can effectively inhibit the activity of xanthine oxidase, thereby reducing the production of uric acid in the body. [2] In the prior art, there are many studies on the extraction process of total plant polyphenols, but there is no report on the extraction process of phenolic compounds in peach branch medicinal materials.

[0003] Based on this, the present invention designs a method for extracting total polyphenols from peach twig medicinal materials, and uses ethanol solution as an extraction solvent to ultrasonically extract total polyphenols from peach twig medicinal materials. By investigating the extraction process, the optimal extraction process conditions for total polyphenols from peach twig medicinal materials are obtained: the extraction solvent is ethanol, the extraction method is ultrasonic extraction, the extraction time is 80min, the solid-liquid ratio is 1:40g / mL, and the ethanol volume fraction is 60%. Under this condition, the maximum yield of total polyphenols from peach twig medicinal materials is 7.454±0.04mg / g. The extraction process is simple, efficient, and has a high yield of total polyphenols, which provides strong support for the quality assessment of peach twig medicinal materials and the development and utilization of medicinal materials. Summary of the invention

[0004] The purpose of the present invention is to provide a method for extracting total polyphenols from peach branch medicinal materials.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] The method for extracting total polyphenols from peach branch medicinal materials of the present invention comprises the following steps:

[0007] 1) Cut the peach branch medicinal material into small pieces, dry them in an oven at 50-60°C for 24-48 hours, crush them, pass them through a 40-100 mesh sieve to obtain peach branch medicinal material fine powder, and seal them for later use;

[0008] 2) Take peach branch medicinal material fine powder, add 40-80% ethanol solution at a solid-liquid ratio of 1:30-1:50 g / mL, and perform ultrasonic extraction for 50-90 minutes.

[0009] Preferably, in the extraction method of the present invention, step 1) is: cutting the peach branch medicinal material into small pieces, drying in an oven at 50-55° C. for 30-42 hours, crushing, passing through a 40-80 mesh sieve to obtain peach branch medicinal material fine powder, and sealing for standby use.

[0010] Further preferably, in the extraction method described in the present invention, step 1) is: cutting the peach branch medicinal material into small pieces, drying in an oven at 55° C. for 36 hours, crushing, passing through a 40-mesh sieve to obtain peach branch medicinal material fine powder, and sealing for later use.

[0011] Preferably, in the extraction method of the present invention, step 2) is: taking peach branch medicinal material fine powder, adding 50-80% ethanol solution at a solid-liquid ratio of 1:30-1:45 g / mL, and ultrasonically extracting for 60-90 minutes.

[0012] Further preferably, in the extraction method of the present invention, step 2) is: taking peach branch medicinal material fine powder, adding 50-70% ethanol solution at a solid-liquid ratio of 1:35-1:40 g / mL, and ultrasonically extracting for 70-80 minutes.

[0013] More preferably, in the extraction method of the present invention, step 2) is: taking peach branch medicinal material fine powder, adding 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and ultrasonically extracting for 80 minutes.

[0014] In the extraction method of the present invention, the temperature of ultrasonic extraction is 40-60°C.

[0015] Preferably, in the extraction method of the present invention, the temperature of ultrasonic extraction is 50°C.

[0016] In the extraction method of the present invention, the power of ultrasonic extraction is 60W and the frequency is 35-40kHz.

[0017] Preferably, in the extraction method of the present invention, the power of ultrasonic extraction is 60W and the frequency is 40kHz.

[0018] Beneficial effects of the present invention:

[0019] The present invention provides a method for extracting total polyphenols from peach twig medicinal materials, and uses ethanol solution as an extraction solvent to ultrasonically extract total polyphenols from peach twig medicinal materials. By investigating the extraction process, the optimal extraction process conditions for total polyphenols from peach twig medicinal materials are obtained: the extraction solvent is ethanol, the extraction method is ultrasonic extraction, the extraction time is 80 minutes, the solid-liquid ratio is 1:40g / mL, and the ethanol volume fraction is 60%. Under this condition, the maximum yield of total polyphenols from peach twig medicinal materials is 7.454mg / g. The extraction process is simple, efficient, and has a high yield of total polyphenols, which provides strong support for the quality assessment of peach twig medicinal materials and the development and utilization of medicinal materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is the gallic acid standard curve;

[0021] Figure 2 The effect of different material-liquid ratios on the yield of total polyphenols from peach twig medicinal materials;

[0022] Figure 3 The effect of different ethanol volume fractions on the total polyphenol yield of peach branch medicinal materials;

[0023] Figure 4 is the effect of different extraction times on the yield of total polyphenols;

[0024] Figure 5 These are the results of total polyphenol content determination in 28 batches of peach branch medicinal materials. DETAILED DESCRIPTION

[0025] The technical solution of the present invention is described in detail below in conjunction with specific embodiments. The following embodiments are only used for explanation and illustration, and do not constitute a limitation on the technical solution of the present invention.

[0026] Example 1

[0027] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0028] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0029] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 80 min.

[0030] Example 2

[0031] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0032] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0033] 2) Peach branch medicinal material powder was added into 50% ethanol solution at a solid-liquid ratio of 1:25 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 50 min.

[0034] Example 3

[0035] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0036] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0037] 2) Peach branch medicinal material powder was added into 50% ethanol solution at a solid-liquid ratio of 1:50 g / mL, and extracted by ultrasound (power 60 W, frequency 40 kHz) at 50° C. for 50 min.

[0038] Example 4

[0039] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0040] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0041] 2) Peach branch medicinal material powder was added into 40% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 50 min.

[0042] Example 5

[0043] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0044] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0045] 2) Peach branch medicinal material powder was added into 80% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 50 min.

[0046] Example 6

[0047] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0048] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0049] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 50 min.

[0050] Example 7

[0051] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0052] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0053] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted by ultrasound (power 60 W, frequency 40 kHz) at 50° C. for 90 min.

[0054] Example 8

[0055] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0056] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 60°C and dry them for 24 hours, crush them, pass them through a 100-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0057] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 80 min.

[0058] Example 9

[0059] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0060] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 50°C and dry them for 48 hours, crush them, pass them through an 80-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0061] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 40 kHz) for 80 min.

[0062] Example 10

[0063] The extraction of total polyphenols from peach branch medicinal materials is as follows:

[0064] 1) Cut the peach branch medicinal material into small pieces, put them into an oven at 55°C and dry them for 36 hours, crush them, pass them through a 40-mesh sieve, and obtain peach branch medicinal material fine powder, which is then sealed for later use.

[0065] 2) Peach branch medicinal material powder was added into 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and extracted at 50° C. with ultrasound (power 60 W, frequency 35 kHz) for 80 min.

[0066] In order to further verify the reliability of the present invention and screen out the best solution, the inventor conducted a series of experiments, as follows:

[0067] 1 Experimental Materials

[0068] 1.1 Peach branch medicinal material sample

[0069] After professional identification by Mr. Yang Ye from the Key Laboratory of Pharmacognosy of Chinese Medicine of Guizhou University of Traditional Chinese Medicine, it was confirmed that the 28 batches of peach twig medicinal materials provided were all dried branches of peach of the genus Prunus in the Rosaceae family. The 28 batches of peach twig medicinal materials were cut into small pieces, placed in an oven at 55°C for 36 hours, crushed, passed through a 40-mesh sieve, sealed and stored for later use. The batch information is shown in Table 1, and the TZ-8 batch was selected for total polyphenol extraction condition screening.

[0070] Table 1 Information on 28 batches of peach branch medicinal materials

[0071]

[0072] 1.2 Experimental instruments

[0073] The experimental instruments are shown in Table 2.

[0074] Table 2 Experimental instruments

[0075]

[0076]

[0077] 1.3 Experimental reagents

[0078] The experimental reagents are shown in Table 3.

[0079] Table 3 Experimental reagents

[0080]

[0081] 2 Experimental methods

[0082] 2.1 Solution preparation

[0083] 2.1.1 Preparation of test samples

[0084] Accurately weigh 1.000g of peach branch medicinal material powder and place it in a 150mL conical flask. Then, accurately measure 25mL of 50% ethanol solution by volume and perform ultrasonic extraction for 50min. After the extract is cooled to room temperature, filter it to obtain the test solution.

[0085] 2.1.2 Preparation of reference solution

[0086] Accurately weigh 1.000 mg of gallic acid and place it in a 10 mL brown volumetric flask. Add 5 mL of distilled water to the volumetric flask and shake it thoroughly to mix it completely. After confirming that the gallic acid has been completely dissolved, make up the volume to obtain a gallic acid solution with a concentration of 0.1 mg / mL.

[0087] 2.1.3 Preparation of 20% sodium carbonate solution

[0088] Accurately weigh 4.000 g of anhydrous sodium carbonate powder and place it in a 50 mL flask. Accurately add 20 mL of distilled water and stir until completely dissolved to obtain a 20% sodium carbonate solution (prepare and use immediately).

[0089] 2.1.4 Color development and determination method

[0090] Use a pipette to accurately draw 50μL of the test solution in Item 2.1.1 into a 20mL brown volumetric flask, and use a pipette to sequentially add 1mL of Folin phenol reagent and 2mL of 20% sodium carbonate solution to the reaction container. After the two reagents are mixed in the container, a chemical reaction will occur. Let it continue for 3 minutes, and then add distilled water to the container until the liquid level reaches the calibration mark of the volumetric flask to complete the volume. In order to make the reaction more complete, place the container at room temperature and let it stand for 30 minutes. After the reaction is completed, use a pipette to accurately extract 200μL of the reaction solution, then inject it into a 96-well plate and measure the absorbance value at a wavelength of 760nm.

[0091] 2.2 Extraction condition setting

[0092] According to literature review and combined with the polyphenol extraction process, in the preliminary experimental design, this study first used a solid-liquid ratio of 1:25 (g / mL) and 50% ethanol solution as the extraction solvent to extract total polyphenols from peach twig medicinal materials under ultrasonic heating at 50°C for 50 minutes.

[0093] 2.3 Standard curve inspection

[0094] Use a pipette to accurately draw 0.2, 0.3, 0.4, 0.5, and 0.6 mL of the reference substance in 2.1.2, and place the samples in 10 mL brown volumetric flasks, then perform color development according to the method described in 2.1.4, and measure the absorbance value. After the determination is completed, use the obtained absorbance data as the ordinate and the concentration of the gallic acid reference solution (mg / mL) as the abscissa to draw a standard curve.

[0095] 2.4 Determination of total polyphenol content in peach branch medicinal materials

[0096] Take 50 μL of peach branch liquid in a 20 mL brown bottle, measure the absorbance according to 2.1.4, substitute the polyphenol concentration into the standard curve, and then calculate the total polyphenol yield of peach branch medicinal materials in different batches:

[0097]

[0098] In the formula: W is the sample weight of peach twig medicinal material (g); n is the dilution factor; C is the total polyphenol concentration of the standard curve (mg / mL); V is the volume of the peach twig medicinal material test solution absorbed (mL).

[0099] 2.5 Optimization experiment of extraction process of total polyphenols from peach twig

[0100] In the process of in-depth exploration of the optimization of the extraction process, the experimental conditions were carefully set, including the precise control of the extraction temperature at 50°C, the stabilization of the ultrasonic power at 60W, and the consistency of the ultrasonic frequency (40KHz). On this basis, the three core variables of solid-liquid ratio, ethanol extraction concentration and extraction time were investigated to determine the effects on the total polyphenol extraction efficiency. To ensure the accuracy of the experimental results, three rigorous repeatability experiments were conducted on each single variable. Finally, the average of the absorbance values ​​obtained from the three experiments was used to accurately calculate the extraction rate of total polyphenols.

[0101] 2.5.1 Investigation of extraction solvent and extraction method

[0102] Extract the total polyphenols of peach twigs according to the method under 2.1.1, take samples and add 50% methanol and ethanol respectively, perform ultrasonic extraction, perform color development and determine the absorbance value according to the conditions of 2.1.4, and calculate the total polyphenol content of peach twigs.

[0103] Take a sample and add a solvent of known concentration, perform ultrasonic extraction and reflux extraction respectively, perform color development and determine the absorbance value under the conditions of 2.1.4, and calculate the total polyphenol content of peach branch medicinal materials.

[0104] 2.5.2 Investigation of material-liquid ratio

[0105] The total polyphenols of peach twig were extracted by fixing the extraction method and extraction solvent according to the preparation conditions in 2.1.1, and the colorimetric determination was performed according to the method in 2.1.4. The absorbances at solid-liquid ratios of 1:30, 1:35, 1:40, 1:45 and 1:50 (g / mL) were measured, and the total polyphenol yield of peach twig was calculated.

[0106] 2.5.3 Investigation of ethanol volume fraction

[0107] The solid-liquid ratio (g / mL) was fixed at 1:40, and other conditions remained unchanged. The preparation of the test sample in 2.1.1 was followed to implement the extraction process of total polyphenols in peach twig medicinal materials. In this process, 5 different volume fractions of ethanol solutions were adopted, specifically 40, 50, 60, 70, and 80%, for extraction operations. After the extraction was completed, the color reaction and determination were carried out as described in 2.1.4. Next, the extract was filtered and its absorbance was measured, and based on this, the extraction yield of total polyphenols was calculated.

[0108] 2.5.4 Investigation of extraction time

[0109] Accurately weigh 1.000g of peach branch medicinal material powder. Follow the peach branch medicinal material test solution preparation method detailed in 2.1.1 for extraction. In order to further explore the effect of extraction time on the extraction effect, extraction was carried out at different time points (50, 60, 70, 80 and 90min), and the color development and determination process specified in 2.1.4 was strictly followed. The absorbance of the extract at each time point was accurately measured to calculate the total polyphenol yield of peach branch medicinal material.

[0110] 2.6 Experimental treatment

[0111] The above experiments were repeated three times, and the data were processed using Origin and other software. The results are expressed as X±SD.

[0112] 3 Results Analysis

[0113] 3.1 Drawing of standard curve

[0114] The linear regression equation of mass concentration and absorbance value was obtained by experiment: A = 0.0691X + 0.0351 (R 2 =0.9994), the result is as follows Figure 1 shown.

[0115] 3.2 Effects of extraction methods and different extraction solvents on the yield of total polyphenols

[0116] The experiment adopted the controlled variable method, first fixed the extraction conditions as follows: extraction time 50min, solid-liquid ratio 1:25 (g / mL), and investigated the effects of extraction methods and different extraction solvents on the yield of total polyphenols from peach branches. The results showed that when the extraction solvent was ethanol, the yield of total polyphenols obtained by ultrasonic extraction was 4.348±0.008mg / g; the yield of total polyphenols obtained by reflux extraction was 4.234±0.013mg / g, so the extraction method selected in the experiment was ultrasonic extraction. The different extraction solvents methanol and ethanol were further investigated. The results showed that under ultrasonic extraction conditions, the total polyphenol yield measured when the extraction solvent was methanol was 3.887±0.018mg / mL, so ethanol was selected as the extraction solvent in the experiment.

[0117] 3.3 Effect of solid-liquid ratio on total polyphenol yield

[0118] This study used 50 minutes as the extraction time and set the volume fraction of ethanol to 50%, and then focused on exploring the influence of different solid-liquid ratios on the total polyphenol extraction efficiency. The results showed that when the solid-liquid ratio was between 1:30 and 1:40 (g / mL), the total polyphenol yield trended upward. When the solid-liquid ratio reached 1:40 (g / mL), the total polyphenol yield of peach branch medicinal materials reached a peak value; when it exceeded 1:40 to 1:50 (g / mL), the total polyphenol yield tended to decrease. The results screened out the best solid-liquid ratio of 1:40 (g / mL). The results are as follows Figure 2 shown.

[0119] 3.4 Effect of ethanol volume fraction on total polyphenol yield

[0120] When the extraction time was set to 50 min and the solid-liquid ratio was 1:40 (g / mL), the effect of ethanol volume fraction on the yield of polyphenols in peach twig medicinal materials was explored. The results showed that when the volume fraction of ethanol gradually increased in the range of 40-60%, the extraction rate of total polyphenols in peach twig medicinal materials also increased. In particular, when the volume fraction of ethanol reached the critical point of 60%, the extraction rate of total polyphenols climbed to the highest point. However, as the volume fraction of ethanol increased further from 60% to 80%, the extraction rate of total polyphenols declined instead. It is further speculated that this may be due to the presence of certain alcohol-soluble impurities in peach twig medicinal materials, which compete with polyphenols for solvents, thereby affecting the effective dissolution of polyphenols. Based on this finding, an ethanol volume fraction of 60% was determined as the most ideal extraction condition to maximize the extraction efficiency of total polyphenols in peach twig medicinal materials. The results are as follows Figure 3 shown.

[0121] 3.5 Effect of extraction time on total polyphenol yield

[0122] In the setting data of solid-liquid ratio of 1:40 (g / mL) and ethanol volume fraction of 60%, the effect of extraction time on the yield of polyphenols in peach branch medicinal material was deeply explored.

[0123] After in-depth analysis of the result data, it was found that within the extraction time range of 50 to 80 minutes, the extraction rate of total polyphenols in peach branch medicinal materials showed a positive correlation with the extraction time. Specifically, as the extraction time increased, the extraction rate of total polyphenols gradually increased, and when the extraction time reached 80 minutes, the extraction rate reached its highest point. However, as the extraction time was extended from 80 minutes to 90 minutes, the extraction rate showed a downward trend. This change may be due to the damage caused to the total polyphenol components by the excessively long ultrasonic extraction time, which in turn affected the extraction effect. Based on the above data, it can be concluded that the extraction time has an important influence on the extraction rate of total polyphenols, and an extraction time of 80 minutes can achieve the best extraction effect of total polyphenols. At the same time, the degree of influence on total polyphenols is relatively low, so the extraction time is set to 80 minutes. The results are as follows Figure 4 shown.

[0124] 3.6 Determination results of total polyphenol content in different batches of peach branch medicinal materials

[0125] Under the optimal extraction conditions, this study conducted a detailed content determination of 28 different batches of peach twig medicinal materials. Through in-depth analysis of these data, it was found that the peach twig medicinal materials of batch TZ-15 showed the highest total polyphenol extraction rate, significantly better than all other batches, with a yield of 7.454±0.04mg / g; the batch with the lowest yield was TZ-2, with a total polyphenol yield of 2.136±0.013mg / g. The results are shown in Table 4. Figure 5 .

[0126] Table 4 Determination of total polyphenol content in 28 batches of peach branch medicinal materials (X±SD)

[0127]

[0128] References:

[0129] [1] Chinese Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China. Volume 1[M]. China Medical Science and Technology Press, 2020: 291.

[0130] [2] Xu Huimin. Study on quality evaluation of green-calyx plum based on antioxidant and xanthine oxidase inhibition activity[D]. Anhui University of Traditional Chinese Medicine, 2024.

Claims

1. A method for extracting total polyphenols from peach branch medicinal materials, characterized in that: The following steps are involved: 1) Cut the peach branch medicinal material into small pieces, dry them in an oven at 50-60°C for 24-48 hours, crush them, pass them through a 40-100 mesh sieve to obtain peach branch medicinal material fine powder, and seal them for later use; 2) Take peach branch medicinal material fine powder, add 40-80% ethanol solution at a solid-liquid ratio of 1:30-1:50 g / mL, and perform ultrasonic extraction for 50-90 minutes.

2. The extraction method according to claim 1, characterized in that The step 1) is as follows: cutting the peach branch medicinal material into small pieces, drying in an oven at 50-55° C. for 30-42 hours, crushing, passing through a 40-80 mesh sieve to obtain peach branch medicinal material fine powder, and sealing for later use.

3. The extraction method according to claim 2, characterized in that The step 1) is as follows: cutting the peach branch medicinal material into small pieces, drying in an oven at 55° C. for 36 hours, crushing, passing through a 40-mesh sieve, obtaining peach branch medicinal material fine powder, and sealing for later use.

4. The extraction method according to claim 1, characterized in that The step 2) is as follows: taking peach branch medicinal material fine powder, adding 50-80% ethanol solution at a solid-liquid ratio of 1:30-1:45 g / mL, and ultrasonically extracting for 60-90 minutes.

5. The extraction method according to claim 4, characterized in that The step 2) is as follows: taking peach branch medicinal material fine powder, adding 50-70% ethanol solution at a solid-liquid ratio of 1:35-1:40 g / mL, and ultrasonically extracting for 70-80 minutes.

6. The extraction method according to claim 5, characterized in that The step 2) is as follows: taking peach branch medicinal material fine powder, adding 60% ethanol solution at a solid-liquid ratio of 1:40 g / mL, and ultrasonically extracting for 80 minutes.

7. The extraction method according to any one of claims 1, 4, 5 or 6, characterized in that: The temperature of the ultrasonic extraction is 40-60°C.

8. The extraction method according to claim 7, characterized in that The temperature of the ultrasonic extraction is 50°C.

9. The extraction method according to any one of claims 1 or 4 or 5 or 6, characterized in that The power of the ultrasonic extraction is 60W and the frequency is 35-40kHz.

10. The extraction method according to claim 9, characterized in that The power of the ultrasonic extraction is 60W and the frequency is 40kHz.

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