Sleep-aiding fragrance composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of application of natural products, and particularly relates to a sleep-aiding fragrance composition as well as a preparation method and application thereof. The sleep-aiding fragrance composition comprises the following raw materials: white tea essential oil, cinnamomum camphora essential oil, kumquat flower essential oil, honey pomelo essential oil, michelia alba essential oil and sandalwood essential oil. The compound essential oil disclosed by the invention has good effects of synergistically soothing the nerves and improving sleep, and the aroma is highly accepted by people. In addition, the compound essential oil does not contain artificially synthesized essence, preservatives, mineral grease and chemical components causing allergy, the components are safe, and allergy is not prone to occurring.

Description

Technical Field

[0001] The present invention belongs to the technical field of natural product application, and in particular relates to a sleep-aiding fragrance composition and a preparation method and application thereof. Background Art

[0002] Insomnia refers to difficulty falling asleep, waking up easily and early in the middle of sleep, poor sleep quality, significantly reduced sleep time, and some serious patients can not sleep all night. Long-term insomnia can easily cause upset, fatigue, and even headaches, nightmares, sweating, memory loss, and can also cause a series of clinical symptoms and induce some psychosomatic diseases. Nowadays, people are under great pressure at work and have more things to think about, which causes the brain to be active when people sleep, or causes insomnia due to anxiety. Many people use drugs to assist sleep, but drugs cannot be used for a long time. Long-term use will not only produce dependence, but also make the brain slow to react and have adverse effects on the body. It is often manifested as: insufficient sleep time and depth lead to the inability to eliminate fatigue, restore physical strength and energy. In mild cases, it is difficult to fall asleep, or sleep but not soundly, wake up from sleep, or cannot fall asleep again after waking up, and in severe cases, stay awake all night.

[0003] At present, the methods for treating insomnia include drug therapy, muscle relaxation, hypnosis, suggestion therapy, behavioral therapy, etc. At present, the main medical treatment is drug therapy, but the current clinical drugs for the treatment of insomnia mainly include melatonin receptor agonists, benzodiazepine receptor agonists and antidepressants with hypnotic effects, etc. However, long-term use of the above drugs usually has strong side effects, such as dizziness, slow reaction or drowsiness.

[0004] Aromatherapy products, such as aromatherapy liquid, ointment, aromatherapy candles, etc., are prepared by adding essential oils with aromatic odors into a certain carrier. In existing aromatherapy products, the essential oils used are mostly prepared by chemically synthesized essences. However, such chemically synthesized essential oil components will cause harm to the air and human body when volatilized.

[0005] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants. Essential oils enter the nasal cavity through deep breathing. When breathing, the essential oil molecules in the air will be carried to the olfactory cells at the top of the nose, and the cilia in the cells will remember and transmit the fragrance, and then pass through the olfactory valve to the olfactory area of ​​the brain. The chemicals in the essential oils trigger the release of neurochemicals, which produce calming, relaxing or exciting effects. Essential oils will also enter the lungs and enter the blood circulation through gas exchange.

[0006] Studies have shown that medicinal materials containing more Chinese herbal essential oils, such as Acorus calamus, Albizzia julibrissin, rose, frankincense, cypress seed, jujube seed, sandalwood, etc., have a good effect of promoting sleep. Chinese invention patent application CN101632761A discloses a compound tranquilizing oil and Chinese herbal essential oil and aromatherapy products with tranquilizing effects. The compound tranquilizing oil is composed of 15%-25% Acorus calamus extract, 2%-7% star anise extract, 1%-3% Nepeta extract, 0.5%-1.5% eucalyptus extract and 65%-80% camphor leaf extract; the Chinese herbal essential oil includes by weight: 30%-60% compound tranquilizing oil, 30%-60% lavender essential oil and 5%-15% peppermint oil. The prepared Chinese herbal essential oil has a fragrant smell and has the effects of sedation, sleep aid and tranquilization. However, the essential oil of Acorus calamus contains safrole, which has a certain carcinogenic effect, limiting its scope of application.

[0007] Therefore, it is extremely important to find products that are purely natural, safe, and have no toxic side effects to improve sleep quality. Summary of the invention

[0008] In view of the deficiencies in the prior art, the present invention provides a sleep-aiding fragrance composition and a preparation method and application thereof.

[0009] In order to achieve the above-mentioned purpose of the present invention, the specific technical solution adopted by the present invention is:

[0010] A sleep-aiding fragrance composition, the raw materials of which include white tea essential oil, camphor essential oil, kumquat flower essential oil, honey pomelo essential oil, white jasmine essential oil and sandalwood essential oil.

[0011] Preferably, the raw materials of the sleep-aiding fragrance composition include, by volume: 4-6 parts of white tea essential oil, 14-16 parts of camphor essential oil, 0.8-1.2 parts of kumquat essential oil, 14-16 parts of honey pomelo essential oil, 7-8 parts of white jasmine essential oil and 6-7 parts of sandalwood essential oil.

[0012] Further preferably, the raw materials of the sleep-aiding fragrance composition include, by volume: 5 parts of white tea essential oil, 15 parts of camphor essential oil, 1 part of kumquat flower essential oil, 15 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0013] Preferably, the white tea essential oil, camphor tree essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil and sandalwood essential oil are volatile oils prepared from white tea leaves, camphor tree leaves, kumquat flowers, pomelo peels, white jasmine and sandalwood as raw materials, respectively, by steam distillation, low-polarity organic solvent extraction, carbon dioxide supercritical extraction, supramolecular distillation or pressing.

[0014] Preferably, the raw materials of the sleep-aiding fragrance composition further include auxiliary materials, and the auxiliary materials are selected from one or more of water, ethanol, dipropylene glycol and glycerin.

[0015] Further preferably, the volume ratio of the total volume of the white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil and sandalwood essential oil to the auxiliary material is 1:1-100, preferably 1:50-75.

[0016] Preferably, the method of using the sleep-aiding fragrance composition includes any one of aromatherapy inhalation, spraying, diffusion, application and massage.

[0017] The present invention also relates to a method for preparing the above-mentioned sleep-aiding fragrance composition, comprising the following steps:

[0018] The white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil, sandalwood essential oil and auxiliary materials are mixed, stirred and allowed to stand to obtain the sleep-aiding fragrance composition.

[0019] The present invention also relates to the use of the sleep-aiding fragrance composition in the preparation of sleep-aiding products.

[0020] Preferably, the sleep aid product is used to treat, prevent or improve symptoms of insomnia, dreaminess, waking during sleep and waking up easily in the middle of the night.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention rationally mixes white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil and sandalwood essential oil, and combines a specific ratio, so that the obtained compound essential oil has good synergistic effects of calming the nerves and improving sleep, and the fragrance is highly accepted by the public. In addition, the compound essential oil of the present invention does not contain artificial synthetic essence, does not add preservatives, does not contain mineral oils and fats, does not contain chemical components that cause allergies, has safe ingredients, and is not prone to allergies. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a graph showing the results of gas chromatography-mass spectrometry (GC-MS) testing of the sleep-aiding fragrance composition of Example 1 of the present invention. DETAILED DESCRIPTION

[0024] The present invention will be further described in detail below in conjunction with specific examples. The following examples are not intended to limit the present invention, but are only intended to illustrate the present invention. The experimental methods used in the following examples are generally conventional, unless otherwise specified, and the materials, reagents, etc. used in the following examples are commercially available, unless otherwise specified.

[0025] The raw material essential oil of the sleep-aiding fragrance composition can be directly purchased, or it can be extracted from the original plant as the raw material using the conventional method of extracting essential oils, and the conventional method of extracting essential oils includes but is not limited to steam distillation, pressing, supercritical extraction, chemical solvent extraction, oil separation (lipid absorption), or soaking. In a preferred embodiment, the white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white orchid essential oil and sandalwood essential oil are respectively made of white tea leaves, camphor leaves, kumquat flowers, pomelo peels, white orchids and sandalwood as raw materials, and their volatile oils are extracted by steam distillation. Steam distillation is a conventional method for extracting essential oils, which is not described in detail here.

[0026] Example 1

[0027] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 5 parts of white tea essential oil, 15 parts of camphor essential oil, 1 part of kumquat flower essential oil, 15 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0028] Example 2

[0029] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 4 parts of white tea essential oil, 14 parts of camphor essential oil, 0.8 parts of kumquat flower essential oil, 14 parts of honey pomelo essential oil, 7 parts of white jasmine essential oil and 6 parts of sandalwood essential oil.

[0030] Example 3

[0031] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 6 parts of white tea essential oil, 16 parts of camphor essential oil, 1.2 parts of kumquat flower essential oil, 16 parts of honey pomelo essential oil, 8 parts of white jasmine essential oil and 7 parts of sandalwood essence.

[0032] Example 4

[0033] Preparation of an aromatherapy volatile liquid:

[0034] The sleep-aiding fragrance composition in Example 1 was mixed with ethanol in a volume ratio of 1:60, stirred thoroughly, and allowed to stand at room temperature for 7 days to be fully blended.

[0035] After testing, the aromatherapy volatile liquid prepared by the present invention has a lead content of less than 5.0 mg / kg, an arsenic content of less than 2.5 mg / kg, and a mercury content of less than 0.1 mg / kg, all of which meet the standard requirements of GB7916-1987.

[0036] Comparative Example 1

[0037] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 35 parts of white tea essential oil, 0 parts of camphor essential oil, 1 part of kumquat flower essential oil, 0 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0038] Comparative Example 2

[0039] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 0 parts of white tea essential oil, 17.5 parts of camphor essential oil, 1 part of kumquat flower essential oil, 17.5 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0040] Comparative Example 3

[0041] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 5 parts of white tea essential oil, 30 parts of camphor essential oil, 1 part of kumquat flower essential oil, 0 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0042] Comparative Example 4

[0043] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 5 parts of white tea essential oil, 0 parts of camphor essential oil, 1 part of kumquat flower essential oil, 30 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

[0044] Comparative Example 5

[0045] A sleeping-aiding fragrance composition comprises the following raw materials, measured by volume: 7 parts of white tea essential oil, 12 parts of camphor essential oil, 1 part of kumquat flower essential oil, 15 parts of honey pomelo essential oil, 10 parts of white jasmine essential oil and 5 parts of sandalwood essential oil.

[0046] Effect test

[0047] Test Example 1 Component Analysis

[0048] 0.5 mL of the sleep-aiding fragrance composition in Example 1 was transferred into a 1.5 mL centrifuge tube and centrifuged for 1 min; 50 μL of the supernatant was taken, weighed, and ethyl acetate was added to prepare an essential oil solution with a concentration of 100 mg / mL; anhydrous sodium sulfate powder was added at a mass ratio of 1:10, mixed, allowed to stand, and centrifuged; 10 μL of the supernatant was taken, diluted 100 times with ethyl acetate to obtain a test solution with a concentration of 1 mg / mL; the test solution was centrifuged for 1 min, the supernatant was taken, and detection was performed by gas chromatography-mass spectrometry.

[0049] The detection conditions were as follows: chromatographic column: Agilent HP-5MS UI (30m×0.25mm×0.25μm); carrier gas: high-purity helium; flow rate: 1.0mL / min; injection port temperature: 250℃; split ratio: 10:1; injection volume: 1μL; heating program: 50℃-180℃ (3℃ / min)-300℃ (20℃ / min, hold for 5min); ion source: EI + ; Ion source temperature: 230℃; Scan mode: full ion scan; Scan range: m / z 40-600; Solvent delay: 4min.

[0050] The raw data of GC-MS detection were analyzed by Agilent MassHunter Qualitative Analysis (VB.07.00) software to obtain the information of each component peak (retention time, integrated peak area, peak area percentage); the structure of each component peak was assigned by matching the mass spectrum database (NIST 2020V2.4) and the retention index library (NIST Chemistry WebBook). The results are as follows Figure 1 And as shown in Table 1.

[0051] Table 1 Information of component peaks

[0052]

[0053]

[0054] Test Example 2 Animal Experiment

[0055] SD mice, clean grade, half male and half female, weighing 18g-20g. After 1 week of adaptive feeding, the mice were randomly divided into a blank control group, Example 1-3 groups, and Comparative Example 1-5 groups, with 10 mice in each group.

[0056] Experimental method: The blank control group inhaled distilled water by nebulization, and the Example 1-3 group and the Comparative Example 1-5 group inhaled the sleep-aiding fragrance composition described in the Example 1-3 and the Comparative Example 1-5 respectively. For 7 consecutive days, 30 minutes each time, and 100 μL each time. On the 7th day, all groups of mice inhaled the corresponding substances by nebulization, and then injected pentobarbital solution (50 mg / kg) intraperitoneally. The tail of the mouse was lifted, and the mouse was laid flat on the pad, and the nebulization administration was continued. The sleep latency was from the time of intraperitoneal injection to the disappearance of the righting reflex of the mouse, and the duration of the righting reflex was the sleep time. The sleep latency time and sleep time were recorded, and the results are shown in Table 2.

[0057] Data processing: The measurement data were expressed as mean ± standard deviation, and the data were processed by one-way analysis of variance. P < 0.05 indicated statistical significance.

[0058] Table 2 Effects of sleep-aiding fragrance composition on sleep of mice

[0059] Group Sleep latency time (min) Sleep time (min) Blank control group 4.61±0.35 40.12±0.59 Example 1 <![CDATA[2.05±0.21 * ]]> <![CDATA[62.39±0.57 * ]]> Example 2 <![CDATA[2.57±0.24 * ]]> <![CDATA[60.13±1.05 * ]]> Example 3 <![CDATA[2.36±0.19 * ]]> <![CDATA[61.34±0.92 * ]]> Comparative Example 1 <![CDATA[4.22±0.12 *# ]]> <![CDATA[43.64±0.85 *# ]]> Comparative Example 2 <![CDATA[3.95±0.21 *# ]]> <![CDATA[45.40±1.16 *# ]]> Comparative Example 3 <![CDATA[4.05±0.11 *# ]]> <![CDATA[44.51±0.83 *# ]]> Comparative Example 4 <![CDATA[4.14±0.17 *# ]]> <![CDATA[44.07±1.05 *# ]]> Comparative Example 5 <![CDATA[4.18±0.15 *# ]]> <![CDATA[43.96±1.12 *# ]]>

[0060] Note: Compared with the blank control group, * P<0.05; compared with the Example 1 group, # P<0.05.

[0061] Test Example 3: Human sleep aid efficacy test

[0062] Subjects: A total of 104 people, 22 males, 82 females, aged 20-72 years old, average age 44±14 years old, including:

[0063] 18-35 years old, 12 males, 23 females, aged 20-35 years, average age 28±4 years;

[0064] 35-55 years old, 3 males and 32 females, aged 38-55 years, with an average age of 46±5 years;

[0065] 55 years and above, 7 males and 27 females, aged 56-72 years, average age 59±4 years;

[0066] Test method: The subjects were tested for EEG before sniffing and while continuously sniffing the aromatherapy volatile liquid in Example 4, and the data of the proportion of theta waves in the brain wave channels T3 (left temporal lobe) and T4 (right temporal lobe) were statistically analyzed.

[0067] Test equipment: Digital EEG topograph, XL-998C, 16 channels, Jiangsu Mutai Medical Technology Co., Ltd.

[0068] Environmental conditions: temperature 20-25℃, humidity 50-60%RH.

[0069] Test process: The subjects wait quietly for 15 minutes in an environment of 20-25℃; wear brain wave monitoring equipment, monitor for 5 minutes, and continuously smell the test substance for 10 minutes using the aromatherapy button method, while monitoring for 10 minutes.

[0070] Statistics:

[0071] Descriptive statistics were performed on the data of the percentage of theta waves in the T3 (left temporal lobe) and T4 (right temporal lobe) EEG channels;

[0072] The data of the percentage of theta waves in the EEG channels of T3 (left temporal lobe) and T4 (right temporal lobe) were tested for normal distribution. If the data were normally distributed, the paired T test was used for statistical analysis; if the test data were non-normally distributed, the rank sum test method was used for statistical analysis. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05.

[0073] If the proportion of △θ waves is >0, it has a sleep-aiding effect; if the proportion of △θ waves is ≤0, it has no sleep-aiding effect.

[0074] The test results are shown in Table 3.

[0075] Table 3 Test results of human sleep-aiding efficacy

[0076]

[0077]

[0078] Note: Compared with the proportion of theta waves before sniffing, ** P<0.01, there is a very significant difference.

[0079] The test data results show that compared with the time period when the sleep-aiding fragrance composition in Example 1 was not inhaled, there was a significant difference in the proportion of theta waves in the T3 (left temporal lobe) and T4 (right temporal lobe) EEG channels after inhaling the sleep-aiding fragrance composition, indicating that it has a significant sleep-aiding effect.

[0080] Test Example 4: Survey on the Acceptance of Fragrance by the Public

[0081] 100 volunteers were selected, and none of the volunteers had respiratory diseases, colds, or rhinitis symptoms. The sleep-aiding fragrance compositions obtained in Examples 1-3 and Comparative Examples 1-5 were subjected to odor tests. Before the tests, the compositions in Examples 1-3 and Comparative Examples 1-5 were respectively mixed with ethanol at a volume ratio of 1:50 to obtain aromatherapy products. The evaluation results are shown in Table 4.

[0082] Table 4 Odor acceptance evaluation results

[0083] Group Satisfied (people) General (people) Dissatisfied (people) Example 1 95 3 2 Example 2 89 8 3 Example 3 90 6 4 Comparative Example 1 76 15 9 Comparative Example 2 80 13 7 Comparative Example 3 78 16 6 Comparative Example 4 81 12 7 Comparative Example 5 77 16 7

[0084] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A sleeping-aiding fragrance composition, characterized in that: The raw materials of the sleep-aiding fragrance composition include: white tea essential oil, camphor essential oil, kumquat flower essential oil, honey pomelo essential oil, white jasmine essential oil and sandalwood essential oil.

2. The sleep-aiding fragrance composition according to claim 1, characterized in that: The raw materials of the sleep-aiding fragrance composition include, by volume: 4-6 parts of white tea essential oil, 14-16 parts of camphor essential oil, 0.8-1.2 parts of kumquat essential oil, 14-16 parts of honey pomelo essential oil, 7-8 parts of white jasmine essential oil and 6-7 parts of sandalwood essential oil.

3. The sleep-aiding fragrance composition according to claim 2, characterized in that: The raw materials of the sleep-aiding fragrance composition include, by volume: 5 parts of white tea essential oil, 15 parts of camphor essential oil, 1 part of kumquat flower essential oil, 15 parts of honey pomelo essential oil, 7.5 parts of white jasmine essential oil and 6.5 parts of sandalwood essential oil.

4. The sleep-aiding fragrance composition according to claim 1, characterized in that: The white tea essential oil, camphor tree essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil and sandalwood essential oil are volatile oils prepared by using white tea leaves, camphor tree leaves, kumquat flowers, pomelo peels, white jasmine and sandalwood as raw materials, respectively, through steam distillation, low-polarity organic solvent extraction, carbon dioxide supercritical extraction, supramolecular distillation or pressing.

5. The sleep-aiding fragrance composition according to claim 1, characterized in that: The raw materials of the sleep-aiding fragrance composition also include auxiliary materials, and the auxiliary materials are selected from one or more of water, ethanol, dipropylene glycol and glycerin.

6. The sleep-aiding fragrance composition according to claim 5, characterized in that: The volume ratio of the total volume of the white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil and sandalwood essential oil to the auxiliary material is 1:1-100.

7. The sleep-aiding fragrance composition according to claim 6, characterized in that: The method of using the sleep-aiding fragrance composition includes any one of aromatherapy inhalation, spraying, diffusing, applying and massaging.

8. A method for preparing the sleep-aiding fragrance composition according to any one of claims 5 to 7, characterized in that: The following steps are involved: The white tea essential oil, camphor essential oil, kumquat flower essential oil, pomelo essential oil, white jasmine essential oil, sandalwood essential oil and auxiliary materials are mixed, stirred and allowed to stand to obtain the sleep-aiding fragrance composition.

9. Use of the sleep-aiding fragrance composition according to any one of claims 1 to 7 in the preparation of a sleep-aiding product.

10. The use according to claim 9, characterized in that: The sleep aid product is used to treat, prevent or improve the symptoms of insomnia, dreaminess, waking up during sleep and waking up easily in the middle of the night.

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