Anti-aging life-prolonging and qi-tonifying decoction composition and application thereof

Abstract

The anti-aging life-prolonging and qi-tonifying decoction composition comprises the following raw material medicines or extracts of the raw material medicines in parts by weight: 9-30 parts of radix pseudostellariae, 9-30 parts of poria with hostwood, 6-20 parts of angelica sinensis, 6-20 parts of fried spina date seeds, 6-15 parts of radix ophiopogonis, 3-15 parts of roasted polygala tenuifolia, 10-60 parts of longan aril, 10-30 parts of radix rehmanniae, 9-30 parts of radix scrophulariae, 3-20 parts of rhizoma acori graminei, 9-30 parts of semen boitae and 15-30 parts of roasted tortoise plastron. , 15-30 parts of calcined dragon bone, 6-30 parts of lotus, and 3-60 parts of licorice. The traditional Chinese medicine composition can inhibit the activity of an aging marker beta-galactosidase in cells and animal bodies and the expression of P16 or P21, eliminate aging cells, improve the oxidation resistance of the cells and animals and reduce the inflammatory factor expression level of the cells and animals, and has a remarkable anti-aging effect.

Description

Technical Field

[0001] This invention relates to the fields of medicine and cosmetics, specifically to an anti-aging longevity-boosting and qi-tonifying decoction composition and its uses. Background Technology

[0002] The statements in this section provide only background information relevant to the disclosure of this application and may not constitute prior art.

[0003] Aging is a degenerative change in the body's overall function that occurs with age. It is an extremely complex biological process, involving mechanisms such as gene mutations, telomere loss, somatic cell mutations, free radical damage, immune dysfunction, mitochondrial dysfunction, and autophagy dysfunction. Cardiovascular disease, cancer, cataracts, osteoporosis, hypertension, and neurodegenerative diseases such as Alzheimer's and Parkinson's are all related to aging. Against the backdrop of an aging population, many countries have entered aging societies, posing a serious threat to economic development and human health. Therefore, research into anti-aging drugs is urgently needed.

[0004] Studies have shown that drug therapy can effectively delay aging and has a positive effect on age-related diseases. Aspirin and metformin are commonly used synthetic anti-aging drugs, but these drugs also have significant side effects. Aspirin has an antiplatelet aggregation effect, and long-term use can easily cause bleeding. Patients taking metformin may experience adverse reactions such as diarrhea, nausea, and abdominal discomfort. The discovery and development of anti-aging drugs is difficult and progresses slowly; finding a safe and effective anti-aging drug is challenging. In recent years, traditional Chinese medicine (TCM) has been considered a safe and effective anti-aging drug with great development potential. Research has found that many TCMs and their active ingredients can delay aging and prevent age-related diseases. The main mechanisms of action of TCM are anti-oxidation and anti-inflammation, and different mechanisms of action have been determined according to different organs and functions. Many single and compound TCMs have shown beneficial anti-aging effects in various parts and functions of the body. To realize the industrialization of TCM, fully utilize the advantages of TCM, and develop safe, effective, and innovative anti-aging and longevity-enhancing decoction preparations with independent intellectual property rights, is of great significance for promoting the internationalization of my country's health industry and improving the quality of life of Chinese residents.

[0005] Therefore, an anti-aging, longevity-boosting and qi-tonifying soup composition and its uses are invented. Summary of the Invention

[0006] The purpose of this invention is to address the current shortage of anti-aging longevity-boosting and qi-tonifying decoction compositions, and to provide an anti-aging longevity-boosting and qi-tonifying decoction composition and its uses, which can significantly inhibit aging in cell models and animal models.

[0007] The technical solutions of the present invention are as follows:

[0008] A longevity-enhancing and qi-tonifying decoction composition for anti-aging, characterized in that it comprises the following raw materials or extracts thereof in parts by weight: Codonopsis pilosula 9-30 parts, Poria cocos 9-30 parts, Angelica sinensis 6-20 parts, stir-fried Ziziphus jujuba var. spinosa 6-20 parts, Ophiopogon japonicus 6-15 parts, processed Polygala tenuifolia 3-15 parts, longan pulp 10-60 parts, Rehmannia glutinosa 10-30 parts, Scrophularia ningpoensis 9-30 parts, Acorus tatarinowii 3-20 parts, Platycladus orientalis 9-30 parts, processed tortoise shell 15-30 parts, calcined dragon bone 15-30 parts, lotus flower 6-30 parts, and licorice root 3-60 parts.

[0009] Preferably, the active pharmaceutical ingredient or extract thereof comprises the following parts by weight: 15-25 parts of Codonopsis pilosula, 15-25 parts of Poria cocos, 15-20 parts of Angelica sinensis, 15-20 parts of stir-fried Ziziphus jujuba var. spinosa, 10-15 parts of Ophiopogon japonicus, 7-15 parts of processed Polygala tenuifolia, 5-15 parts of longan pulp, 15-25 parts of Rehmannia glutinosa, 15-25 parts of Scrophularia ningpoensis, 10-20 parts of Acorus tatarinowii, 15-25 parts of Platycladus orientalis, 15-25 parts of processed tortoise shell, 15-25 parts of calcined dragon bone, 6-11 parts of lotus flower, and 3-11 parts of licorice root.

[0010] Preferably, the active pharmaceutical ingredient or extract thereof comprises the following parts by weight: 17-23 parts of Codonopsis pilosula, 17-23 parts of Poria cocos, 17-20 parts of Angelica sinensis, 17-20 parts of stir-fried Ziziphus jujuba var. spinosa, 12-15 parts of Ophiopogon japonicus, 9-15 parts of processed Polygala tenuifolia, 7-13 parts of longan pulp, 17-23 parts of Rehmannia glutinosa, 17-23 parts of Scrophularia ningpoensis, 12-18 parts of Acorus tatarinowii, 17-23 parts of Platycladus orientalis, 17-23 parts of processed tortoise shell, 17-23 parts of calcined dragon bone, 6-9 parts of lotus flower, and 3-9 parts of licorice root.

[0011] According to a preferred embodiment, the lotus flower is lotus petals that have been picked fresh and freeze-dried for 3-5 days, or air-dried for 2-4 weeks.

[0012] According to a preferred embodiment, the aging is characterized by increased activity of the in vivo aging markers β-galactosidase and high expression of P16 or P21.

[0013] This application also provides an anti-aging pharmaceutical preparation, including the anti-aging longevity-boosting and qi-tonifying decoction composition as described above.

[0014] According to a preferred embodiment, the aging is characterized by increased activity of the in vivo aging markers β-galactosidase and high expression of P16 or P21.

[0015] According to a preferred embodiment, it also includes one or more pharmaceutically acceptable excipients.

[0016] According to a preferred embodiment, it can be formulated as a topical or oral preparation.

[0017] This application also provides the use of the anti-aging longevity-tonifying decoction composition as described above in the preparation of anti-aging traditional Chinese medicine or cosmetics.

[0018] The anti-aging longevity and qi-tonifying decoction composition or pharmaceutical preparations and cosmetics prepared from the longevity and qi-tonifying decoction composition provided by this invention can be applied to mammals, including but not limited to humans, rats, horses, pigs, dogs, cattle, sheep, rabbits, etc.

[0019] Compared with existing technologies, the advantages of this invention are:

[0020] 1. An anti-aging longevity and qi-tonifying decoction composition, which is non-toxic to HaCat cells, promotes HaCat cell proliferation and enhances cell activity, significantly reduces the expression of HaCat cell senescence markers P16 and P21 genes, induces apoptosis of HaCat senescent cells and thus clears senescent cells, improves the antioxidant capacity of HaCat cells, reduces the expression level of HaCat cell inflammatory factors, and can significantly inhibit cell senescence.

[0021] 2. An anti-aging longevity and qi-tonifying decoction composition, demonstrated through animal behavioral experiments, shows that the longevity and qi-tonifying decoction composition of the present invention can improve the spatial learning and memory abilities of aging mice, delay the aging of the kidneys of mice, significantly reduce the expression of aging markers P16 and P21 genes in mice, improve the antioxidant capacity of mice, and reduce the expression level of inflammatory factors in mice, thus having an anti-aging effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The effect of Yishou Buqi Decoction on HaCat senescent cells: Compared with the model group, the blank group showed ##P<0.01; compared with the 500μg / mL drug administration group, the model group showed **P<0.01.

[0023] Figure 2 The effect of the Yishou Buqi Decoction of this application on the activity of normal HaCat cells. Note: Compared with the drug-treated group, **P<0.01;

[0024] Figure 3 Flow cytometry scatter plots of HaCat cells in different groups according to this application;

[0025] Figure 4 The statistical analysis of apoptotic cells in HaCat cells of different groups after 24 hours is shown in the figure. Compared with the model group, the blank group showed ##P<0.01; compared with the 500μg / mL drug administration group, the model group showed **P<0.01.

[0026] Figure 5To investigate the effects of Yishou Buqi Decoction on the expression of aging markers P16 and P21, note: (A) P16 expression map of HaCat cells in different groups; (B) P21 expression map of HaCat cells in different groups. Compared with the model group, the blank group... ## P < 0.01; Compared with 125 μg / mL and 500 μg / mL, **P < 0.01;

[0027] Figure 6 The effect of Yishou Buqi Decoction on the antioxidant capacity of HaCaT cells: comparison between the blank group and the model group. ## P < 0.01; Compared with 62.5 μg / mL, 125 μg / mL, 250 μg / mL, 500 μg / mL and 1000 μg / mL, **P < 0.01, *P < 0.05;

[0028] Figure 7 The effects of Yishou Buqi Decoction on HaCaT cell inflammatory factors: Compared with the model group, the blank group... ## P < 0.01, ### P < 0.001; Compared with 500 μg / mL, **P < 0.01, ***P < 0.001 in the model group;

[0029] Figure 8 Kidney β-galactosidase staining sections of mice in different groups. Note: (A) Blank group; (B) Model group; (C) Low-dose group; (D) High-dose group;

[0030] Figure 9 Effects of Yishou Buqi Decoction on P16 and P21 expression in vivo. Note: (A) P16 expression in mice of different groups; (B) P21 expression in mice of different groups. Compared with the model group, the blank group... ## P < 0.01; Compared with the low-dose group and the high-dose group, **P < 0.01;

[0031] Figure 10 Effects of Yishou Buqi Decoction on Antioxidant Capacity in D-Galactose-Induced Aging Mice. Compared with the model group, the control group... # P < 0.05 ## P < 0.01; Compared with the high-dose group, *P < 0.05, **P < 0.01;

[0032] Figure 11 The effect of Yishou Buqi Decoction on inflammatory factors in D-galactose-induced aging mice: Compared with the model group, the blank group... ## P < 0.01, ### P < 0.001; Compared with the low-dose group, high-dose group and positive control group (VE), *P < 0.05, **P < 0.01, ****P < 0.0001;

[0033] Figure 12 Comparative Example 1 and Example 1: DPPH, ABTS + Comparison with FRAP radical scavenging ability;

[0034] Figure 13 The effects of Comparative Example 1 and Example 1 on HaCaT cell survival were compared with those of the control group and the drug-treated group. *P<0.05, **P<0.01;

[0035] Figure 14 Comparative Example 2 and Example 1: DPPH, ABTS + Comparison with FRAP radical scavenging ability;

[0036] Figure 15 The effect of Comparative Example 2 and Example 1 on HaCaT cell survival rate was compared with that of the control group and the drug-treated group. **P<0.01. Detailed Implementation

[0037] The features and performance of the present invention will be further described in detail below with reference to embodiments. Unless otherwise specified, the methods mentioned in this application are commonly used methods in the art; unless otherwise specified, the experimental materials used in this application are all commercially available.

[0038] Example 1: Preparation of water extract samples

[0039] Weigh out the following Chinese herbal medicine slices according to the following dosages: Codonopsis pilosula 20g, Poria cocos 20g, Angelica sinensis 20g, stir-fried Ziziphus jujuba var. spinosa 20g, Ophiopogon japonicus 15g, Polygala tenuifolia 12g, longan aril 10g, Rehmannia glutinosa 20g, Scrophularia ningpoensis 20g, Acorus tatarinowii 15g, Platycladus orientalis 20g, stir-fried tortoise shell 20g, calcined dragon bone 20g, lotus flower 6g, licorice root 6g;

[0040] Mix the above-mentioned Chinese herbs in the specified proportions, add 10 times the total mass of distilled water, soak for 30 minutes, and then decoct three times, adding 10 times the total mass of distilled water each time. Combine the decoctions from the three decoctions, filter out the dregs, and finally concentrate the filtered decoction using a rotary evaporator and then freeze-dry it. Accurately weigh 50 mg of the sample, dissolve it in DMEM culture medium to a concentration of 50 mg / mL, vortex mix, dilute with the culture medium to the required concentration, and filter through a 0.22 μm microporous membrane for cell experiments.

[0041] Example 2: In vitro evaluation of the anti-aging activity of the longevity-boosting and qi-tonifying decoction composition

[0042] Experimental cells: HaCat cells (human immortalized keratinocytes), divided into a blank control group, a model group, and a drug-treated group. The blank control group consisted of HaCat cells with culture medium, the model group consisted of HaCat cells irradiated with ultraviolet light for 30 seconds, and the drug-treated group consisted of cells irradiated with ultraviolet light for 30 seconds, then supplemented with 7.8125, 15.625, 31.25, 62.5, 125, 250, 500, and 1000 μg / mL of traditional Chinese medicine extract.

[0043] 1. In vitro experiments

[0044] 1.1 Validation of the cellular senescence model

[0045] First, irradiate the cells with a UV lamp at a distance of 15 cm for 30 seconds, then replace with fresh DMEM medium and continue culturing. After 24 hours of culture, use Xite... TM β-D-galactopyranoside staining was used to detect senescent cells. Figure 1 It was observed that after the Yishou Buqi Decoction was administered to the cells, the FITC fluorescence intensity decreased significantly, indicating a reduction in the number of senescent cells within the cells. Simultaneously, there was a significant difference between the model group and the control group, indicating the successful establishment of the senescence model. This demonstrates that the Yishou Buqi Decoction reduces the number of senescent cells and has a senescent cell-clearing effect.

[0046] 1.2 Effects of Yishou Buqi Decoction on Normal Cells

[0047] After combining the Yishou Buqi Decoction with other ingredients, CCK8 assay results showed that cell activity was not decreased compared to the control group. Furthermore, cell activity was enhanced under high drug concentrations. Figure 2 This indicates that the drug is not toxic to cells, and that high concentrations of the drug promote the proliferation of normal HaCat cells, thereby enhancing their cell activity.

[0048] 1.3 Longevity-Boosting Decoction Induces Apoptosis in HaCat Senescent Cells

[0049] Flow cytometry analysis results of the effect of Yishou Buqi Decoction on apoptosis in HaCat senescent cells are as follows: Figure 3 and Figure 4 As shown, cells were divided into three groups: control group, model group, and drug-treated group (500 μg / mL). After 24 hours of drug treatment on senescent cells, as... Figure 3 It is clearly visible that the number of cells in regions Q2 and Q3 of the drug-treated group is significantly increased. Figure 4 It can be seen that, compared with the blank group and the model group, the apoptosis rate of the drug-treated group was significantly increased, showing a statistically significant difference. Therefore, Yishou Buqi Decoction can induce apoptosis in HaCat senescent cells and thus eliminate senescent cells.

[0050] 1.4 Yishou Buqi Decoction inhibits the expression of p16 and p21 in vitro.

[0051] P16 and P21 are typical biomarkers of aging, and both are highly expressed in senescent cells. Real-time PCR was used to detect the expression of P16 and P21 in cells treated with Yishou Buqi Decoction. Figure 5 As shown in A, P16 expression was high in the model group, and the ability to inhibit P16 expression increased with increasing drug concentration in the drug-treated group. Similarly, from Figure 5 As shown in Figure B, P21 was highly expressed in the model group, and the ability to inhibit P21 expression increased with increasing drug concentration in the drug-treated group. Therefore, it can be concluded that Yishou Buqi Decoction inhibits the expression of aging genes P16 and P21.

[0052] 1.5 Effects of Yishou Buqi Decoction on the Antioxidant Capacity of HaCaT Cells

[0053] Continuous contact between the human body and the external environment generates excessive free radicals, disrupting the balance between the body's own antioxidants and free radicals, thus accelerating aging. Figure 6 It can be seen that with the increase of drug concentration, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cells increased significantly, while the content of malondialdehyde (MDA) decreased significantly, indicating that Yishou Buqi Decoction improved the antioxidant capacity of cells.

[0054] 1.6 Effects of Yishou Buqi Decoction on HaCaT Cell Inflammatory Factors

[0055] Depend on Figure 7 It can be seen that when Yishou Buqi Decoction was administered to cells, the levels of intracellular IL-6 (interleukin-6), TNF-α (tumor necrosis factor), and IL-1β (interleukin-1β) decreased significantly, indicating that Yishou Buqi Decoction reduced the expression of intracellular inflammatory factors.

[0056] Example 3: Evaluation of the anti-aging activity of traditional Chinese medicine in mice.

[0057] Seventy-two healthy C57BL / 6 mice, weighing 18-20g each, half male and half female, were used. They were fed a normal diet and were acclimatized for one week before the experiment was conducted.

[0058] C57BL / 6 mice were randomly divided into 6 groups: blank control group, model group, vitamin E (VE) group, and low, medium, and high dose drug groups. Each mouse in the blank control group was intraperitoneally injected with 0.3 mL of physiological saline, while the other groups were intraperitoneally injected with an equal volume of D-galactose (0.75 g of D-galactose accurately weighed and dissolved in 25 mL of 0.9% physiological saline) once daily for 6 consecutive weeks to establish the model.

[0059] After successful model establishment, mice in the low, medium, and high dose groups were administered 0.3 mL of the drug (200, 400, and 800 mg / kg respectively) via gavage. The vitamin E group was administered an equal volume of vitamin E (100 mg / kg), while the control and model groups were administered an equal volume of physiological saline. This was done once daily for 5 consecutive weeks. Mouse weight was measured and recorded weekly, and coat color, activity level, and mental state were observed.

[0060] 1.1Y Maze Analysis

[0061] Mice were placed in a Y-maze, and the proportion of time spent entering the new or different arm was calculated. The results showed that mice with successful modeling spent less time entering the new or different arm than mice in the control group, while mice in the drug-treated group spent significantly more time entering the new or different arm compared to the model group, indicating that Yishou Buqi Decoction improves the spatial learning and memory abilities of aging mice (as shown in Table 1).

[0062] Table 1. Percentage of mice from different groups entering the new heteroarm (%) (x±s, n=7)

[0063]

[0064] Note: Different lowercase letters in the same column indicate significant differences, and the p-value between two letters is <0.05.

[0065] 1.2β-galactosidase stained sections

[0066] Since the kidneys are one of the important organs of aging, we analyzed β-galactosidase stained sections of the kidneys of mice in different groups. Figure 8 (A) is the blank group; (B) is the model group; (C) is the low-dose group; (D) is the high-dose group. Figure 8 It can be seen that compared with the blank group (A), the model group (B) showed a significant increase in positive staining, and the number of positive staining decreased significantly with increasing dosage. This indicates that Yishou Buqi Decoction can effectively delay kidney aging.

[0067] 1.3 Yishou Buqi Decoction inhibits the expression of P16 and P21 in vivo.

[0068] The kidneys are vital organs in the aging process. RNA was extracted from the kidneys of mice in different groups by grinding, and then subjected to RT-PCR experiments. Figure 9 As shown in A, P16 was highly expressed in the model group, and the ability to inhibit P16 expression increased with increasing drug dosage. Figure 9 As shown in B, P21 was highly expressed in the model group, and the ability to inhibit P21 expression increased with increasing drug dosage in the treatment group. Therefore, it can be concluded that Yishou Buqi Decoction also inhibits the expression of aging genes P16 and P21 in vivo.

[0069] 1.4 Effects of Yishou Buqi Decoction on Antioxidant Capacity in D-Galactose-Induced Aging Mice

[0070] Depend on Figure 10 It can be seen that high-dose Yishou Buqi Decoction significantly increased the activity of superoxide dismutase (SOD) and catalase (CAT) in the kidneys of mice, and significantly decreased the content of malondialdehyde (MDA). Moreover, there was a significant difference between the model group and the blank group, indicating that Yishou Buqi Decoction improved the antioxidant capacity of aging mice.

[0071] 1.5 Effects of Yishou Buqi Decoction on Inflammatory Factors in D-Galactose-Induced Aging Mice

[0072] Depend on Figure 11 It was found that when mice were administered different doses of Yishou Buqi Decoction by gavage, the levels of IL-6 (interleukin-6), TNF-α (tumor necrosis factor), and IL-1β (interleukin-1β) in the mice decreased significantly with the dosage, and the differences were statistically significant, indicating that Yishou Buqi Decoction reduced the expression of inflammatory factors in mice.

[0073] Comparative Example 1

[0074] A traditional Chinese medicine composition for nourishing the mind and calming the nerves was prepared, consisting of the following ingredients: 25.5g of stir-fried jujube seeds, 26.4g of raw dragon bone, 17g of schisandra fruit, 16.5g of polygala root, 8g of red ginseng, 16.5g of poria cocos, 12.5g of raw rehmannia root, 11g of ophiopogon root, 25.2g of lily bulb, 6.5g of angelica root, 5.8g of longan pulp, 12.3g of white peony root, 20g of processed tortoise shell, 9g of albizia bark, 26.7g of polygonum multiflorum stem, 7.5g of magnetite, and 1.2g of amber.

[0075] Using the same preparation method as in Example 1, the above-mentioned drugs were soaked in water for half an hour, decocted three times, and the decoctions from the three decoctions were concentrated using a rotary evaporator. Finally, the concentrated liquid was freeze-dried.

[0076] 1.1 Antioxidant activity

[0077] Depend on Figure 12 It is evident that when the drug concentration reaches 1000 μg / mL, the DPPH radical scavenging rate of Comparative Example 1 reaches 80%, but the DPPH radical scavenging rate of Example 1 reaches 96%. Compared with Comparative Example 1, the DPPH radical scavenging rate of Example 1 is stronger.

[0078] When the drug concentration reaches 1000 μg / mL, ABTS in Example 1 + The free radical scavenging rate reached 96%. Figure 12 It can be seen that ABTS at various concentrations in Example 1 + The free radical scavenging rates were all higher than those of control example 1.

[0079] Depend on Figure 12 It can be seen that the FRAP free radical scavenging rate at all concentrations in Example 1 is higher than that in Comparative Example 1. In summary, the overall antioxidant capacity of Example 1 is significantly stronger than that of Comparative Example 1.

[0080] 1.2 Cytotoxicity

[0081] Depend on Figure 13 It was found that Comparative Example 1 was toxic to cells at concentrations of 500 μg / mL and above, and cell viability decreased by 25% at a concentration of 1000 μg / mL. Example 1 was not toxic to cells, and at concentrations of 500 μg / mL and above, it had a proliferative effect on cells, increasing cell viability by 7%.

[0082] In summary, the compound formulation of this application, with fewer types of compound drugs, increases antioxidant activity without producing cytotoxicity, and its overall antioxidant effect is superior to that of Comparative Example 1.

[0083] Comparative Example 2

[0084] A traditional Chinese medicine composition for skin anti-aging was prepared, comprising the following substances: 10g saffron extract, 40g ginseng extract, 35g lotus extract, 30g Polygonum cuspidatum extract, 25g Ligusticum chuanxiong extract, and 25g Angelica sinensis extract. Using the same preparation method as in Example 1, the above-mentioned drugs were soaked in water for half an hour, decocted three times, and the decoctions from each decoction were concentrated using a rotary evaporator. Finally, the concentrated liquid was freeze-dried.

[0085] 1.1 Antioxidant activity

[0086] like Figure 14 As can be seen, when the drug concentration reaches 1000 μg / mL, the DPPH free radical scavenging rate of Comparative Example 2 reaches 90%, but the DPPH free radical scavenging rate of Example 1 reaches 96%. Therefore, Example 1 has a stronger DPPH free radical scavenging rate compared to Comparative Example 2.

[0087] like Figure 14 It can be seen that when the drug concentration reaches 1000 μg / mL, the ABTS in Example 1... + The free radical scavenging rate reached 96%, and the ability to scavenge free radicals continuously increased with increasing concentration. Comparative Example 2: ABTS + The free radical scavenging rate showed a slow decreasing trend after 250 μg / mL.

[0088] Depend on Figure 14 It can be seen that the iron ion reducing power of Example 1 at all concentrations is higher than that of Comparative Example 2.

[0089] 1.2 Cytotoxicity

[0090] Further verification of the cytotoxicity of comparison example 2 was conducted. Figure 15 It was found that Comparative Example 2 was toxic to cells at concentrations of 15.625 μg / mL and above, and cell viability decreased by 31% at a concentration of 1000 μg / mL. Example 1 was not toxic to cells, and at concentrations of 500 μg / mL and above, it promoted cell proliferation, increasing cell viability by 7%.

[0091] In summary, the compound formulation of this application has increased antioxidant activity, and its overall antioxidant effect at high concentrations is superior to that of Comparative Example 2, without producing cytotoxicity, while Comparative Example 2 does produce cytotoxicity.

[0092] The embodiments described above merely illustrate specific implementation methods of this application, and while the descriptions are detailed and specific, they should not be construed as limiting the scope of protection of this application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the technical solution of this application, and these modifications and improvements all fall within the scope of protection of this application.

Claims

1. An anti-aging longevity-invigorating qi-replenishing soup composition, characterized in that: The invention comprises the following raw materials or extracts of raw materials in parts by weight: 9-30 parts of Pseudostellariae Radix, 9-30 parts of Poria, 6-20 parts of Angelicae Sinensis, 6-20 parts of stir-fried Ziziphus jujuba seeds, 6-15 parts of Ophiopogon japonicus, 3-15 parts of roasted Polygala tenuifolia, 10-60 parts of longan pulp, 10-30 parts of Rehmannia root, 9-30 parts of Scrophulariae ningpoensis, 3-20 parts of Acorus calamus, 9-30 parts of Platycladus chinensis seeds, 15-30 parts of roasted Tortoise shell, 15-30 parts of calcined Dragon bone, 6-30 parts of Lotus, and 3-60 parts of Licorice.

2. The anti-aging longevity-replenishing qi decoction composition according to claim 1, characterized in that: The lotus is fresh lotus petals picked and freeze-dried for 3-5 days or dried in the shade for 2-4 weeks.

3. The anti-aging longevity-invigorating qi-replenishing decoction composition according to claim 1, characterized in that: The aging is the increase of the activity of β-galactosidase, a marker of aging in the body, and the high expression of P16 or P21.

4. An anti-aging pharmaceutical preparation, characterized in that: It comprises an anti-aging, longevity-promoting and qi-replenishing soup composition as claimed in claim 1 or 2.

5. An anti-aging pharmaceutical preparation according to claim 4, characterized in that: The aging is the increase of the activity of β-galactosidase, a marker of aging in the body, and the high expression of P16 or P21.

6. An anti-aging pharmaceutical preparation according to claim 4, characterized in that: One or more pharmaceutically acceptable excipients are also included.

7. An anti-aging pharmaceutical preparation according to claim 4, characterized in that: It can be prepared as an external preparation or an internal preparation.

8. Use of the anti-aging Yishou Buqi Decoction composition as claimed in claim 1 or 2 in the preparation of anti-aging traditional Chinese medicine or cosmetics.

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