A fermented Chinese medicine product for regulating spleen and stomach function and preparation method thereof

Abstract

The present invention relates to the technical field of fermented Chinese medicine products, and discloses a fermented Chinese medicine product for regulating spleen and stomach function and a preparation method thereof. The fermented Chinese medicine product is prepared by a Chinese medicine extract, a fermentation medium and Lactobacillus paracasei LBR-002 of the same origin of medicine and food, and the mass ratio of the Chinese medicine extract, the fermentation medium and Lactobacillus paracasei LBR-002 is 1000:965:20-90, and the Chinese medicine extract comprises the following components in parts by weight: 25-60 parts of yam, 20-50 parts of liquorice, 20-45 parts of ganoderma lucidum, 20-42 parts of tuckahoe, 20-50 parts of polygonatum, 20-48 parts of codonopsis, 18-25 parts of dendrobium officinale, 8-17 parts of daidaihua, 8-16 parts of American ginseng, 5-20 parts of coix seeds, 6-15 parts of amomum, and 8-20 parts of hawthorn. The fermented Chinese medicine product has the characteristics of effective regulation, green safety and the like to spleen and stomach function, and can significantly improve spleen and stomach function.

Description

Technical Field

[0001] The invention belongs to the technical field of fermented Chinese medicine products and relates to a fermented Chinese medicine product for regulating spleen and stomach functions and a preparation method thereof. Background Art

[0002] With the rapid development of society and the rapid improvement of people's living standards, the level of diet has reached an unprecedented height. However, this has also brought a series of health problems, especially due to overly greasy and irregular diet, which leads to disorders in people's spleen and stomach functions, and symptoms such as indigestion and spleen and stomach fragility. Traditional treatment methods, such as the use of medicines such as Jianwei Xiaoshi tablets, mainly reduce stomach discomfort by reducing gastric acid concentration, but it is difficult to fundamentally treat the disease and achieve the effect of strengthening the spleen and stomach. There are many kinds of spleen and stomach products on the market, but the product quality is uneven, and long-term use may even produce side effects. Therefore, it is particularly important to develop a fermented Chinese medicine product that is truly a medicine and food that can effectively regulate the spleen and stomach function for a long time.

[0003] At the same time, with people's increasing attention to health and the popularization of probiotics knowledge, more and more consumers are beginning to realize the benefits of probiotics such as Lactobacillus paracasei on spleen and stomach health. Therefore, applying Lactobacillus paracasei to fermented Chinese medicine products can meet the market demand for high-quality, healthy products. Summary of the invention

[0004] The invention provides a fermented Chinese medicine product for regulating spleen and stomach functions and a preparation method thereof.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A fermented Chinese medicine product for regulating spleen and stomach function is prepared from a Chinese medicine extract, a fermentation medium and Lactobacillus paracasei LBR-002, wherein the mass ratio of the Chinese medicine extract, the fermentation medium and the Lactobacillus paracasei LBR-002 is 1000:965:20-90, the Chinese medicine extract comprises the following components in parts by weight: 25-60 parts of yam, 20-50 parts of liquorice, 20-45 parts of ganoderma lucidum, 20-42 parts of tuckahoe, 20-50 parts of polygonatum, 20-48 parts of codonopsis pilosula, 18-25 parts of dendrobium officinale, 8-17 parts of daidai hua, 8-16 parts of American ginseng, 5-20 parts of coix seeds, 6-15 parts of amomum, and 8-20 parts of hawthorn; the Lactobacillus paracasei LBR-002 accounts for 2% to 9% of the total mass of the Chinese medicine extract. The above-mentioned Lactobacillus paracasei LBR-002 is a unique strain of Shandong Libang Animal Husbandry Co., Ltd. and is deposited in the General Microbiology Center of China Culture Collection Administration (No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing). The preservation date is March 9, 2023. The classification name is Lactobacillus paracasei and the preservation number is CGMCC No. 26775.

[0007] Preferably, the mass ratio of the Chinese medicine extract, the fermentation medium and the Lactobacillus paracasei LBR-002 is 1000:965:36, and the Chinese medicine extract comprises the following components in parts by weight: 45 parts of yam, 30 parts of licorice, 30 parts of ganoderma lucidum, 30 parts of tuckahoe, 30 parts of polygonatum, 30 parts of codonopsis, 20 parts of dendrobium candidum, 10 parts of daidaihua, 10 parts of American ginseng, 10 parts of coix seeds, 10 parts of amomum, and 10 parts of hawthorn; the Lactobacillus paracasei LBR-002 is 3.6% of the total mass of the Chinese medicine extract. The fermentation medium is 9.65% of the total mass of the Chinese medicine extract, and is composed of the following components by mass fraction: 2% peptone, 1% beef extract powder, 0.8% yeast extract powder, 4% glucose, 0.4% dipotassium hydrogen phosphate, 0.4% triammonium citrate, 1% sodium acetate, 0.04% magnesium sulfate, and 0.01% manganese sulfate.

[0008] Preferably, the viable count of Lactobacillus paracasei LBR-002 in the fermented Chinese medicine product for regulating spleen and stomach function is not less than 3.0×10 8 CFU / ml.

[0009] The present invention also provides a method for preparing a fermented Chinese medicine product for regulating spleen and stomach function, comprising the following steps:

[0010] S1. Streak the suspension of Lactobacillus paracasei LBR-002 on MRS solid medium, and culture at 37°C for 36 hours to isolate a single colony. Pick a single colony and inoculate it into MRS liquid medium, culture at 37°C and 100 r·min-1 for 2 days to obtain the seed liquid for fermentation, and refrigerate it for later use;

[0011] S2. The Lactobacillus paracasei LBR-002 seed solution obtained in S1 was expanded and proliferated, inoculated into MRS liquid medium, and cultured at 37 ° C, 100 r·min-1 for 18 h to obtain a fermentation solution of Lactobacillus paracasei LBR-002;

[0012] S3. The cleaned yam, licorice, ganoderma lucidum, Poria, polygonatum, codonopsis, dendrobium officinale, daidaihua, American ginseng, coix seed, amomum, hawthorn were mixed, water was added to submerge the Chinese medicine, soaked for 3 hours, extracted and concentrated three times, the final concentration of the Chinese medicine extract was 0.14g / ml, filtered through 100 mesh, and the fermentation medium with a total mass of 9.65% of the filtered Chinese medicine extract was added to the above Chinese medicine extract, mixed evenly, sterilized with high pressure steam at 121°C for 20min, and cooled for standby use;

[0013] S4. Take 2% to 9% of the total mass of the Chinese medicine extract from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, add it to the mixture obtained in S3, mix well, adjust the pH value to 5.5-7.5, 28-40°C, and ferment for 72-96h.

[0014] Preferably, step S4: 3.6% of the total weight of the Chinese medicine extract is taken from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, added to the mixture obtained in S3, mixed evenly, adjusted to pH 6.5, 37° C., and fermented for 72 hours to obtain the product.

[0015] The beneficial effects of the present invention are:

[0016] 1. The selected raw materials are all Chinese medicinal materials listed in the catalog of substances that are traditionally both food and Chinese medicinal materials, and are safe and green.

[0017] 2. This invention combines traditional Chinese medicinal materials with modern probiotic fermentation technology, so that the efficacy of Chinese medicinal materials can be fully exerted, and macromolecules can be converted into small molecules that are more easily absorbed by the human body. It has a quick effect, obvious spleen and stomach regulation effect, and long-term effect. At the same time, after probiotic fermentation, the taste is better.

[0018] 3. All components in the present invention are purchased from commercial products. The formulation principle of the product for regulating spleen and stomach function is as follows:

[0019] Chinese Yam: Sweet in taste, neutral in nature, enters the spleen, lung, and kidney meridians. It has the effects of nourishing the spleen and stomach, promoting fluid production and benefiting the lungs, and nourishing the kidneys and consolidating essence. It is often used to treat spleen deficiency, poor appetite, lung deficiency, wheezing and coughing, kidney deficiency, spermatorrhea, and leucorrhea.

[0020] Licorice: sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, and harmonizing various medicines. It is often used to treat spleen and stomach weakness, fatigue, cough and sputum, sore throat, drug or food poisoning, etc.

[0021] Lingzhi: Sweet, slightly bitter, neutral in nature, enters the heart, lung, liver, and kidney meridians. It has the effects of replenishing qi and calming the mind, relieving cough and asthma, and strengthening the spleen and kidney. It is often used to treat restlessness, insomnia, cough due to lung deficiency, and low back pain due to kidney deficiency.

[0022] Poria: sweet, light, neutral in nature, enters the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and removing dampness, strengthening the spleen and calming the mind. It is often used to treat edema, oliguria, spleen deficiency, poor appetite, restlessness, insomnia, and dreaminess.

[0023] Polygonatum sibiricum: sweet in taste, neutral in nature, enters the spleen, lung and kidney meridians. It has the effects of nourishing the spleen and moistening the lungs, replenishing the kidneys and replenishing essence. It is often used to treat spleen deficiency, poor appetite, dry cough, kidney deficiency, low back pain, dizziness and tinnitus.

[0024] Codonopsis: sweet in taste, neutral in nature, enters the spleen and lung meridians. It has the effects of tonifying the spleen and lungs, promoting the production of body fluids and nourishing blood. It is often used to treat symptoms such as spleen deficiency, poor appetite, lung deficiency cough, shortness of breath and fatigue, and blood deficiency and chlorosis.

[0025] Dendrobium candidum: sweet in taste, slightly cold in nature, enters the stomach and kidney meridians. It has the effects of nourishing yin, clearing heat, promoting body fluid and quenching thirst. It is often used to treat symptoms such as insufficient stomach yin, dry mouth and tongue, damage to body fluid due to fever, and thirst due to deficiency heat.

[0026] Daidaihua: bitter, spicy, warm in nature, enters the liver and stomach meridians. It has the effects of soothing the liver and regulating qi, harmonizing the stomach and relieving pain. It is often used to treat liver depression and qi stagnation, chest and flank distension and pain, and epigastric pain.

[0027] American ginseng: sweet, slightly bitter, cool in nature, and enters the heart, lung, and kidney meridians. It has the effects of replenishing qi and nourishing yin, clearing away heat and promoting body fluid. It is often used to treat qi and yin deficiency, heat disease damaging body fluid, dry mouth and tongue, and thirst due to deficiency heat.

[0028] Coix seeds: sweet, light, cool in nature, enter the spleen, stomach, and lung meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and stopping diarrhea, clearing away heat and discharging pus. It is often used to treat edema, oliguria, spleen deficiency diarrhea, damp-heat arthralgia, lung abscess, etc.

[0029] Amomum villosum: spicy, warm in nature, enters the spleen, stomach, and kidney meridians. It has the effects of removing dampness and appetizing, warming the middle and relieving pain, and calming the fetus. It is often used to treat dampness blocking the middle jiao, abdominal distension, loss of appetite, and nausea during pregnancy.

[0030] Hawthorn: sour, sweet, slightly warm, enters the spleen, stomach, and liver meridians. It has the effects of digesting food, removing stagnation, promoting blood circulation and removing blood stasis. It is often used to treat food stagnation, abdominal distension, blood stasis, amenorrhea, dysmenorrhea, etc.

[0031] The compatibility principle of the present invention's formula:

[0032] (1) Sovereign, Minister, Assistant and Messenger: This formula follows the principle of TCM compatibility of sovereign, minister, assistant and messenger, among which Chinese herbs such as yam, liquorice, ganoderma lucidum, poria, polygonatum and codonopsis are sovereign herbs, which play the main therapeutic role; dendrobium officinale, daidaihua and American ginseng are minister herbs, which assist sovereign herbs in enhancing the efficacy; amomum villosum and hawthorn are adjuvant herbs, which harmonize the medicinal properties and alleviate the side effects of some herbs; and coix seed are messenger herbs, which guide the medicinal properties to the affected area.

[0033] (2) Nature and flavor: The nature and flavor of each medicinal material coordinate with each other and work together on the spleen, stomach, lungs, kidneys and other internal organs to achieve the effects of harmonizing qi and blood, nourishing yin and moistening dryness, promoting blood circulation and removing blood stasis, regulating qi and promoting digestion.

[0034] This invention embodies the core theories of TCM, such as the holistic concept, syndrome differentiation and treatment, the principle of compatibility of monarch, minister, assistant and envoy, and the theory of the five elements, and aims to regulate spleen and stomach dysfunction by harmonizing yin and yang and balancing the functions of the internal organs. The theory of the five elements of TCM believes that the human body corresponds to the five elements of nature (gold, wood, water, fire, and earth), and the five elements are mutually generated and restrained, and together maintain the balance of the human body. The medicinal materials in this prescription also embody the ideas of the theory of the five elements. For example, medicinal materials such as yam, licorice, and poria belong to the spleen meridian, which belongs to the earth and can nourish the spleen and stomach; medicinal materials such as ganoderma lucidum and American ginseng belong to the heart meridian and lung meridian, which belong to fire and gold, and can nourish the heart and calm the mind, moisten the lungs and relieve cough; Dendrobium officinale belongs to the stomach meridian and kidney meridian, which belongs to water and earth, and can benefit the stomach and produce fluid, nourish yin and clear heat. The compatibility of these medicinal materials takes into account both the mutual generation relationship and the mutual restraint relationship between the internal organs to achieve the purpose of balancing the functions of the internal organs. DETAILED DESCRIPTION

[0035] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Embodiment 1:

[0037] The invention provides a fermented Chinese medicine product for regulating spleen and stomach functions. The fermented Chinese medicine product for regulating spleen and stomach functions is prepared from a Chinese medicine extract, a fermentation medium and lactobacillus paracasei LBR-002, wherein the mass ratio of the Chinese medicine extract, the fermentation medium and the lactobacillus paracasei LBR-002 is 1000:965:36, and the Chinese medicine extract comprises the following components in parts by weight: 30 parts of yam, 20 parts of liquorice, 20 parts of ganoderma lucidum, 20 parts of tuckahoe, 20 parts of polygonatum, 20 parts of codonopsis pilosula, 18 parts of dendrobium officinale, 8 parts of daidai hua, 9 parts of American ginseng, 5 parts of coix seeds, 6 parts of amomum and 8 parts of hawthorn.

[0038] Embodiment 2:

[0039] The Chinese medicine extract contains the following components in parts by weight: 40 parts of yam, 30 parts of licorice, 30 parts of ganoderma lucidum, 30 parts of tuckahoe, 30 parts of polygonatum, 30 parts of codonopsis pilosula, 20 parts of dendrobium officinale, 10 parts of daidaihua, 10 parts of American ginseng, 10 parts of coix seed, 10 parts of amomum, and 10 parts of hawthorn.

[0040] Embodiment 3:

[0041] The Chinese medicine extract contains the following components in parts by weight: 60 parts of yam, 50 parts of licorice, 40 parts of ganoderma lucidum, 40 parts of tuckahoe, 50 parts of polygonatum, 45 parts of codonopsis pilosula, 22 parts of dendrobium officinale, 15 parts of daidaihua, 16 parts of American ginseng, 20 parts of coix seed, 15 parts of amomum, and 20 parts of hawthorn.

[0042] The preparation methods of Examples 1-3 are specifically as follows:

[0043] S1. Streak the suspension of Lactobacillus paracasei LBR-002 on MRS solid medium, and culture at 37°C for 36 hours to isolate a single colony. Pick a single colony and inoculate it into MRS liquid medium, culture at 37°C and 100 r·min-1 for 2 days to obtain the seed liquid for fermentation, and refrigerate it for later use;

[0044] S2. The Lactobacillus paracasei LBR-002 seed solution obtained in S1 was expanded and proliferated, inoculated into MRS liquid medium, and cultured at 37 ° C, 100 r·min-1 for 18 h to obtain a fermentation solution of Lactobacillus paracasei LBR-002;

[0045] S3. The cleaned yam, licorice, ganoderma lucidum, Poria, polygonatum, codonopsis, dendrobium officinale, daidaihua, American ginseng, coix seed, amomum, hawthorn were mixed, water was added to submerge the Chinese medicine, soaked for 3 hours, extracted and concentrated three times, the final concentration of the Chinese medicine extract was 0.14g / ml, filtered through 100 mesh, and the fermentation medium with a total mass of 9.65% of the filtered Chinese medicine extract was added to the above Chinese medicine extract, mixed evenly, sterilized with high pressure steam at 121°C for 20min, and cooled for standby use;

[0046] S4. Take 3.6% of the total mass of the Chinese medicine extract from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, add it to the mixture obtained in S3, mix well, adjust the pH value to 6.5, ferment at 37°C for 72h, and obtain the product.

[0047] The fermented Chinese medicine product for regulating spleen and stomach function prepared above is used once a day, 100 ml each time.

[0048] Comparative Example 1:

[0049] The Chinese medicine extract contains the following components in parts by weight: 30 parts of yam, 20 parts of licorice, 20 parts of ganoderma lucidum, 20 parts of tuckahoe, 20 parts of polygonatum, 20 parts of codonopsis pilosula, 18 parts of dendrobium officinale, 8 parts of daidaihua, 9 parts of American ginseng, 5 parts of coix seed, 6 parts of amomum, and 8 parts of hawthorn.

[0050] Comparative Example 2:

[0051] The Chinese medicine extract contains the following components in parts by weight: 40 parts of yam, 30 parts of licorice, 30 parts of ganoderma lucidum, 30 parts of tuckahoe, 30 parts of polygonatum, 30 parts of codonopsis pilosula, 20 parts of dendrobium officinale, 10 parts of daidaihua, 10 parts of American ginseng, 10 parts of coix seed, 10 parts of amomum, and 10 parts of hawthorn.

[0052] Comparative Example 3:

[0053] The Chinese medicine extract contains the following components in parts by weight: 60 parts of yam, 50 parts of licorice, 40 parts of ganoderma lucidum, 40 parts of tuckahoe, 50 parts of polygonatum, 45 parts of codonopsis pilosula, 22 parts of dendrobium officinale, 15 parts of daidaihua, 16 parts of American ginseng, 20 parts of coix seed, 15 parts of amomum, and 20 parts of hawthorn.

[0054] The preparation method of Comparative Examples 1-3 is specifically as follows:

[0055] Mix the cleaned yam, licorice, ganoderma lucidum, poria, polygonatum, codonopsis, dendrobium officinale, daidaihua, American ginseng, coix seed, amomum villosum, and hawthorn, add water to submerge the Chinese medicine, soak for 3 hours, extract and concentrate three times, the final concentration of the Chinese medicine extract is 0.14g / ml, filter through 100 mesh, and take the filtered Chinese medicine extract for later use.

[0056] Comparative Example 1-3 provides a traditional Chinese medicine extract, which differs from Examples 1-3 in that the preparation method is different.

[0057] Application effect test

[0058] Common symptoms of spleen and stomach dysfunction in traditional Chinese medicine include spleen and stomach disharmony and spleen and stomach weakness. Spleen and stomach disharmony is mainly manifested by loss of appetite, difficulty in defecation, abdominal distension, lack of energy, etc., while spleen and stomach weakness is mainly manifested by loss of appetite, abdominal distension, fatigue, loose stools, sallow complexion, etc. Based on the above symptoms, an effect observation and follow-up form was formulated, and 30 consumers who voluntarily participated in this study and had some of the above symptoms were organized to use it. The application effect is shown in Table 1.

[0059]

[0060] From Table 1, it can be concluded that Examples 1-3 all have the good effect of regulating spleen and stomach function. After 10-15 days, symptoms such as anorexia, dyspepsia, and lack of energy are significantly alleviated; after 15-20 days, symptoms such as abdominal distension, fatigue, loose stools, and sallow complexion are significantly improved. Comparative Examples 1-3 all have the effect of regulating spleen and stomach function. After 20-25 days, symptoms such as anorexia, dyspepsia, and lack of energy begin to alleviate. After 30-35 days, symptoms such as abdominal distension, fatigue, loose stools, and sallow complexion are improved. After fermentation with Lactobacillus paracasei LBR-002, the Examples are more effective than the Comparative Examples.

[0061] Empirical Cases

[0062] Case 1:

[0063] Mr. Zhou from Guanzhuang, Weicheng, 73 years old, before use: stomach discomfort for more than 5 years, during this period he went to the gastroenterology department of the Municipal Hospital of Traditional Chinese Medicine to see a doctor, gastroscopy showed superficial gastritis, took more than 40 sets of Chinese medicine, the situation changed slightly, and it got worse again if he didn't pay attention to his diet, he couldn't eat peppers, garlic, and leeks, and he drank no less than 10 kinds of Chinese patent medicines for treating stomach problems in succession during the 5 years, but it didn't get better. After use: after using 8 bottles of the invention, the condition began to improve significantly, the appetite began to increase and the nausea was significantly reduced; after 14 bottles, the symptoms were all cured, he didn't have to avoid eating, he could eat sour, sweet, spicy, salty and cold, and the appetite increased significantly. After 50 days, he gained 10 catties, felt physically strong, and was in a good mental state.

[0064] Case 2:

[0065] Ms. Xu, 55 years old, from Jiejiazhuang Village, Jiayue Town, Zhucheng City, Weifang City, before use: had stomach problems for 2 years, dared not eat, had severe acid reflux and burning stomach after meals, had long-term diarrhea, 5-6 times a day, lost weight, insomnia, and had no strength. She had been taking medicine for two years, tried both Western and Chinese medicine, but the effect was not ideal and there was no improvement.

[0066] After use: I started to get better after 2 bottles, my acid reflux symptoms were alleviated, diarrhea was completely improved, I took it once a day or once every two days, and my sleep quality improved. After 9 months of use, I gained 5 pounds. I was very surprised by the effect and have been using it for feedback.

[0067] Example 4: Optimization of the preparation method of a fermented Chinese medicine product for regulating spleen and stomach function

[0068] The optimization of the preparation method is mainly achieved by optimizing the inoculation amount of Lactobacillus paracasei LBR-002, the fermentation starting pH, the fermentation temperature, and the fermentation time in step S4. The color and taste of the Chinese medicine extract after fermentation are used as the evaluation criteria.

[0069] Optimization of inoculation amount of Lactobacillus paracasei LBR-002: 2%, 2.4%, 2.8%, 3.2%, 3.6%, 4%, 4.4%, 4.8%, 5.2%, 5.6%, 6%, 6.4%, 6.8%, 7.2%, 7.6%, 8%, 8.4%, 9% of the total mass of the Chinese medicine extract were taken from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, respectively, and added to the mixture obtained in S3, mixed evenly, adjusted the pH value to 6.5, 37°C, and fermented for 72h. See Table 2.

[0070]

[0071] Optimization of fermentation initial pH: 3.6% of the total mass of the Chinese medicine extract was taken from the fermentation liquid of Lactobacillus paracasei LBR-002 obtained in S2, and added to the mixture obtained in S3, and mixed evenly. The initial pH values ​​were 5.5, 6, 6.5, 7, and 7.5, respectively, at 37°C, and fermented for 72 hours. See Table 3.

[0072] Table 3 Effect of initial pH on color and taste of Chinese medicine extracts

[0073] Initial pH 5.5 6 6.5 7 7.5 color B A A+ A A Taste A- A A+ A A

[0074] Note: The letters A and B represent the evaluation level, and A+ represents the best.

[0075] Fermentation temperature optimization: 3.6% of the total weight of the Chinese medicine extract was taken from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, added to the mixture obtained in S3, mixed evenly, and fermented for 72 h at 28°C, 33°C, 37°C, and 40°C, respectively. See Table 4.

[0076] Table 4 Effect of fermentation temperature on the color and taste of Chinese medicine extracts

[0077] Fermentation temperature ℃ 28 33 37 40 color B A A+ A Taste B A A+ A

[0078] Note: The letters A and B represent the evaluation level, and A+ represents the best.

[0079] Optimization of fermentation time: 3.6% of the total mass of the Chinese medicine extract was taken from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, added to the mixture obtained in S3, mixed evenly, and fermented at 37°C for 64h, 68h, 72h, 76h, 80h, 84h, 88h, 92h, and 96h, respectively. See Table 5.

[0080] Table 5 Effect of fermentation time on the color and taste of Chinese medicine extracts

[0081] Fermentation time 64 68 72 76 80 84 88 92 96 color B A- A+ A+ A A A- A- A- Taste B A A+ A+ A+ A+ A+ A+ A+

[0082] From Table 2-Table 5, it can be concluded that the inoculum amount of Lactobacillus paracasei LBR-002 is 3.6%, the initial pH value of fermentation is 6.5, the fermentation temperature is 37°C, the fermentation time is 72h, and the color and mouthfeel of the fermented Chinese medicine liquid are the best. Therefore, the optimization result of step S4 in the preparation method of a fermented Chinese medicine product for regulating spleen and stomach function is to take 3.6% of the total mass of the Chinese medicine extract from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, add the mixture obtained in S3, mix well, adjust the pH value to 6.5, 37°C, and ferment for 72h.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fermented Chinese medicine product for regulating spleen and stomach function, characterized in that: The traditional Chinese medicine product is prepared from a traditional Chinese medicine extract, a fermentation medium and Lactobacillus paracasei LBR-002, wherein the mass ratio of the traditional Chinese medicine extract, the fermentation medium and the Lactobacillus paracasei LBR-002 is 1000:965:20-90, and the traditional Chinese medicine extract comprises the following components in parts by weight: 25-60 parts of yam, 20-50 parts of licorice, 20-45 parts of ganoderma lucidum, 20-42 parts of tuckahoe, 20-50 parts of polygonatum, 20-48 parts of codonopsis pilosula, 18-25 parts of dendrobium officinale, 8-17 parts of daidaihua, 8-16 parts of American ginseng, 5-20 parts of coix seeds, 6-15 parts of amomum, and 8-20 parts of hawthorn; and the Lactobacillus paracasei LBR-002 accounts for 2% to 9% of the total mass of the traditional Chinese medicine extract.

2. A fermented Chinese medicine product for regulating spleen and stomach function according to claim 1, characterized in that: The mass ratio of the traditional Chinese medicine extract, the fermentation medium and the Lactobacillus paracasei LBR-002 is 1000:965:

36. The traditional Chinese medicine extract comprises the following components in parts by weight: 45 parts of yam, 30 parts of licorice, 30 parts of ganoderma lucidum, 30 parts of tuckahoe, 30 parts of polygonatum, 30 parts of codonopsis pilosula, 20 parts of dendrobium officinale, 10 parts of daisy flower, 10 parts of American ginseng, 10 parts of coix seed, 10 parts of amomum, and 10 parts of hawthorn; the Lactobacillus paracasei LBR-002 accounts for 3.6% of the total mass of the traditional Chinese medicine extract.

3. A fermented Chinese medicine product for regulating spleen and stomach function according to any one of claims 1 to 2, characterized in that: The number of viable Lactobacillus paracasei LBR-002 in the fermented Chinese medicine product for regulating spleen and stomach function is not less than 3.0×10 8 CFU / ml.

4. A fermented Chinese medicine product for regulating spleen and stomach function according to any one of claims 1-2, characterized in that: The fermentation medium accounts for 9.65% of the total mass of the traditional Chinese medicine extract, and is composed of the following components by mass fraction: 2% peptone, 1% beef extract powder, 0.8% yeast extract powder, 4% glucose, 0.4% dipotassium hydrogen phosphate, 0.4% triammonium citrate, 1% sodium acetate, 0.04% magnesium sulfate, and 0.01% manganese sulfate.

5. A method for preparing a fermented Chinese medicine product for regulating spleen and stomach function, characterized in that: The steps include: S1. The suspension of Lactobacillus paracasei LBR-002 was streaked onto MRS solid medium, and cultured at 37°C for 36 hours to separate single colonies. Single colonies were selected and inoculated into MRS liquid medium, and cultured at 37°C and 100 r·min-1 for 2 days to obtain seed solution for fermentation, which was refrigerated for later use; S2. The Lactobacillus paracasei LBR-002 seed solution obtained in S1 was expanded and proliferated, inoculated into MRS liquid medium, and cultured at 37 ° C, 100 r·min-1 for 18 h to obtain a fermentation solution of Lactobacillus paracasei LBR-002; S3. The cleaned yam, licorice, ganoderma lucidum, Poria, polygonatum, codonopsis, dendrobium officinale, daidaihua, American ginseng, coix seed, amomum, hawthorn were mixed, water was added to submerge the Chinese medicine, soaked for 3 hours, extracted and concentrated three times, the final concentration of the Chinese medicine extract was 0.14g / ml, filtered through 100 mesh, and the fermentation medium with a total mass of 9.65% of the filtered Chinese medicine extract was added to the above Chinese medicine extract, mixed evenly, sterilized with high pressure steam at 121°C for 20min, and cooled for standby use; S4. Take 2% to 9% of the total mass of the Chinese medicine extract from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, add it to the mixture obtained in S3, mix well, adjust the pH value to 5.5-7.5, 28-40°C, and ferment for 72-96h.

6. The method for preparing a fermented Chinese medicine product for regulating spleen and stomach function according to claim 5, characterized in that: Step S4: Take 3.6% of the total mass of the Chinese medicine extract from the fermented bacterial liquid of Lactobacillus paracasei LBR-002 obtained in S2, add it into the mixture obtained in S3, mix well, adjust the pH value to 6.5, ferment at 37° C. for 72 hours, and obtain the product.

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