Cleaning method of liquid chromatogram mobile phase suction filter head

Abstract

The invention provides a cleaning method of a liquid chromatography mobile phase suction filter head, and belongs to the field of liquid chromatography accessories. The method comprises the following steps: primarily cleaning a to-be-cleaned liquid chromatography mobile phase suction filter head to obtain a first suction filter head; performing first soaking on the first suction filtration head in an ethanol solution to obtain a second suction filtration head; performing second soaking on the second suction filter head in a dilute acid solution, and determining the second soaking time according to the pollution degree of the to-be-cleaned liquid chromatography mobile phase suction filter head to obtain a third suction filter head; and carrying out post-treatment on the third suction filter head to obtain the clean liquid chromatography mobile phase suction filter head. Through the synergistic cleaning effect of the ethanol solution and the acid solution, bacteria, molds and other microorganisms, impurities and residues attached to the surface of the suction filter head can be efficiently removed, and therefore the purpose of efficiently cleaning the suction filter head is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of liquid chromatography accessories, and in particular to a method for cleaning a liquid chromatography mobile phase suction filter. Background Art

[0002] Liquid chromatograph is an analytical instrument widely used in the fields of chemistry, biology, medicine, etc. Its working principle is to dissolve the sample to be tested, and the mobile phase in the liquid reservoir is pumped into the system by a high-pressure pump. The sample solution enters the mobile phase through the injector and is carried into the chromatographic column (stationary phase) by the mobile phase, and then separated and detected by the chromatographic column. The mobile phase suction filter is an important component in the liquid chromatograph. Its main function is to filter impurities and particulate matter in the mobile phase to prevent impurities from entering the chromatographic column and affecting the separation effect.

[0003] Since the mobile phase may contain various impurities and particulate matter, the suction filter head will gradually become clogged during use, resulting in a reduction in the flow rate of the mobile phase or even complete blockage. In order to solve this problem, the suction filter head needs to be cleaned and maintained. The selection of the cleaning method and the technical level are directly related to the use effect of the instrument and the accuracy of the analysis results. However, there is currently no systematic cleaning method for the liquid chromatography mobile phase suction filter head. Therefore, researching and developing an effective liquid chromatography mobile phase suction filter head cleaning method is of great significance for improving the analytical performance of the liquid chromatograph, extending the service life of the instrument, and ensuring the accuracy and reliability of the analysis results. Summary of the invention

[0004] The present application provides a method for cleaning a liquid chromatography mobile phase suction filter head to solve the following technical problem: how to efficiently clean the liquid chromatography mobile phase suction filter head.

[0005] The present application provides a method for cleaning a liquid chromatography mobile phase suction filter head, the method comprising:

[0006] Preliminarily cleaning the liquid chromatography mobile phase suction filter to be cleaned to obtain a first suction filter;

[0007] soaking the first suction filter head in an ethanol solution to obtain a second suction filter head;

[0008] The second suction filter head is immersed in a dilute acid solution for a second time, and the time of the second immersion is determined according to the contamination degree of the liquid chromatography mobile phase suction filter head to be cleaned, to obtain a third suction filter head;

[0009] The third suction filter is post-processed to obtain a clean liquid chromatography mobile phase suction filter.

[0010] Optionally, determining the second immersion time according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned includes:

[0011] The second soaking time is determined according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned; wherein,

[0012] If the degree of contamination is mild, the soaking time is 30 to 40 minutes;

[0013] If the degree of contamination is moderate, the soaking time is 40 to 50 minutes;

[0014] If the pollution degree is severe, the soaking time is 50 minutes to 60 minutes.

[0015] Optionally, the degree of contamination is determined based on stains, residues, color changes and blockages of the liquid chromatography mobile phase filter to be cleaned.

[0016] Optionally, the dilute acid solution is dilute nitric acid, and the volume concentration of the dilute nitric acid is 1% to 10%.

[0017] Optionally, after the first suction filter head is first immersed in the ethanol solution, the method further comprises:

[0018] Use a soft brush or cotton swab to dip in an appropriate amount of ethanol solution to scrub the surface and pores of the first suction filter head after the first soaking to remove microorganisms on the surface of the first suction filter head.

[0019] Optionally, the first soaking time is 5 min to 10 min.

[0020] Optionally, the volume concentration of the ethanol solution is 60% to 80%.

[0021] Optionally, the liquid chromatography mobile phase suction filter to be cleaned is preliminarily cleaned to obtain a first suction filter, comprising:

[0022] The liquid chromatography mobile phase suction filter to be cleaned is placed in a container, the suction filter is cleaned with pure water or deionized water, and the suction filter is rotated to allow the water to cover the pores to remove dirt and residues on the surface of the suction filter, thereby obtaining a first suction filter.

[0023] Optionally, the third suction filter is post-processed to obtain a clean liquid chromatography mobile phase suction filter, comprising:

[0024] Rinse the third suction filter with deionized water;

[0025] Checking the cleanliness of the third suction filter after flushing;

[0026] If the third suction filter does not meet the cleaning requirements, repeat the cleaning process;

[0027] If the third suction filter meets the cleaning requirement, the third suction filter is dried to obtain a clean liquid chromatography mobile phase suction filter.

[0028] Optionally, the inspection method includes one of the following: visual inspection, water flow test, and weight comparison.

[0029] The above technical solution provided by the embodiment of the present application has the following advantages compared with the prior art:

[0030] The present application provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising: performing a preliminary cleaning on a liquid chromatography mobile phase suction filter head to be cleaned to obtain a first suction filter head; performing a first soaking on the first suction filter head in an ethanol solution to obtain a second suction filter head; performing a second soaking on the second suction filter head in a dilute acid solution, and determining the time of the second soaking according to the degree of contamination of the liquid chromatography mobile phase suction filter head to be cleaned to obtain a third suction filter head; performing post-treatment on the third suction filter head to obtain a clean liquid chromatography mobile phase suction filter head. Ethanol solution is a commonly used disinfectant that can quickly kill bacteria and remove most organic residues. Acidic solution can effectively remove inorganic residues and metal oxides. Through the synergistic cleaning effect of ethanol solution and acidic solution, bacteria, molds and other microorganisms and impurities and residues attached to the surface of the suction filter head can be efficiently removed, and the time of dilute nitric acid soaking can be determined according to the degree of contamination of the suction filter head, so as to improve the cleaning efficiency in a targeted manner and save cleaning time. Thereby achieving the purpose of efficiently cleaning the suction filter head. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0032] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0033] Figure 1 A schematic flow chart of a method for cleaning a liquid chromatography mobile phase filter provided in an embodiment of the present application. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0035] Various embodiments of the present application may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present application; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0036] In addition, in the description of the specification of the present application, the terms "including", "comprising", etc. mean "including but not limited to". In this article, relational terms such as "first" and "second", etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. In this article, "and / or" describes the association relationship of associated objects, indicating that three relationships may exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. Wherein A and B can be singular or plural. In this article, "at least one" means one or more, and "plurality" means two or more. "At least one", "the following at least one item (items)" or similar expressions refer to any combination of these items, including any combination of single items (items) or plural items (items). For example, "at least one of a, b, or c", or "at least one of a, b and c", can both mean: a, b, c, ab (i.e. a and b), ac, bc or abc, where a, b, c can be single or plural, respectively.

[0037] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in this application can be purchased from the market or prepared by existing methods.

[0038] Figure 1A schematic flow chart of a method for cleaning a liquid chromatography mobile phase filter provided in an embodiment of the present application.

[0039] See also Figure 1 The present application provides a method for cleaning a liquid chromatography mobile phase suction filter head, the method comprising:

[0040] S1, preliminarily cleaning the liquid chromatography mobile phase suction filter to be cleaned to obtain a first suction filter;

[0041] In some embodiments, the method of preliminarily cleaning the liquid chromatography mobile phase suction filter to be cleaned to obtain a first suction filter includes: placing the liquid chromatography mobile phase suction filter to be cleaned into a container, cleaning the suction filter with pure water or deionized water, and rotating the suction filter to allow the water to cover the pores, so as to remove dirt and residues on the surface of the suction filter, thereby obtaining the first suction filter.

[0042] S2, soaking the first suction filter head in an ethanol solution to obtain a second suction filter head;

[0043] In some embodiments, after the first suction filter head is first immersed in the ethanol solution, the method further includes: using a soft brush or a cotton swab to dip in an appropriate amount of ethanol solution to scrub the surface and pores of the first suction filter head after the first immersion to remove microorganisms on the surface of the first suction filter head.

[0044] In some embodiments, the first soaking time is 5 min to 10 min.

[0045] In some embodiments, the volume concentration of the ethanol solution is 60% to 80%.

[0046] In some embodiments, the volume concentration of the ethanol solution is 75%.

[0047] Ethanol solution is a commonly used disinfectant that can quickly kill bacteria and remove most organic residues. By soaking in ethanol solution, microorganisms attached to the surface of the suction filter can be effectively killed; by brushing with ethanol solution, microorganisms can be completely removed. Exemplarily, the first soaking time can be 5min, 6min, 7min, 8min, 9min, 10min, etc., and the volume concentration of the ethanol solution can be 60%, 62%, 65%, 70%, 72%, 75%, 78%, 80%, etc.

[0048] S3, soaking the second suction filter in a dilute acid solution for a second time, and determining the time of the second soaking according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned, to obtain a third suction filter;

[0049] In some embodiments, determining the second immersion time according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned includes: determining the second immersion time according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned; wherein, if the contamination degree is mild, the immersion time is 30 minutes to 40 minutes; if the contamination degree is moderate, the immersion time is 40 minutes to 50 minutes; if the contamination degree is severe, the immersion time is 50 minutes to 60 minutes.

[0050] In some embodiments, the contamination degree is determined based on the stains, residues, color changes and blockages of the liquid chromatography mobile phase filter to be cleaned.

[0051] In some embodiments, the dilute acid solution is dilute nitric acid, and the volume concentration of the dilute nitric acid is 1% to 10%.

[0052] It should be noted that the criteria for determining the degree of contamination of the suction filter head are as follows: Mild contamination of the suction filter head means: there are a small amount of particles on the surface of the suction filter head or the color changes slightly, but the overall appearance is relatively clean; moderate contamination means: there are more obvious particles or color changes on the surface of the suction filter head, and there are some small blockages; severe contamination means: there are a large number of particles, obvious discoloration or serious blockages on the surface of the suction filter head.

[0053] Dilute nitric acid is a strong acid that can effectively remove inorganic residues and metal oxides. Using dilute nitric acid to clean the liquid phase suction filter head is intended to remove organic matter, inorganic salts and other impurities on the suction filter head to ensure its good performance and extend its service life. According to the degree of contamination of the suction filter head, the soaking time of dilute nitric acid is determined, so as to improve the cleaning efficiency and save cleaning time. Exemplarily, when the degree of contamination is mild, the soaking time can be 30min, 32min, 35min, 38min, 40min, etc.; when the degree of contamination is moderate, the soaking time can be 40min, 42min, 44min, 48min, 50min, etc.; when the degree of contamination is severe, the soaking time can be 50min, 52min, 55min, 58min, 60min, etc. The volume concentration of dilute nitric acid can be 1%, 3%, 5%, 7%, 9%, 10%, etc.

[0054] S4, post-treating the third suction filter to obtain a clean liquid chromatography mobile phase suction filter.

[0055] In some embodiments, the post-processing of the third suction filter to obtain a clean liquid chromatography mobile phase suction filter includes: rinsing the third suction filter with deionized water; checking the cleanliness of the third suction filter after rinsing; if the third suction filter does not meet the cleaning requirements, repeating the cleaning process; if the third suction filter meets the cleaning requirements, drying the third suction filter to obtain a clean liquid chromatography mobile phase suction filter.

[0056] It should be noted that after soaking with dilute nitric acid, rinse the suction filter head thoroughly with deionized water to ensure that all residual nitric acid is removed, and then check whether the suction filter head is cleaned. If it is not cleaned, repeat the above steps S1 to S3. Place the cleaned suction filter head in a dry and ventilated place and let it dry naturally or use a hair dryer to blow it dry gently. In some embodiments, the inspection method includes one of the following: visual inspection, water flow test, and weight comparison.

[0057] Check the cleaning effect of the suction filter head by visual inspection, water flow test, weight comparison and other methods to ensure that microorganisms, organic matter, inorganic salts and other impurities have been completely removed.

[0058] It should be noted that the visual inspection is specifically: observe the appearance of the suction filter head with the naked eye. If the suction filter head originally has obvious stains, residues or color changes, these characteristics should be significantly reduced or disappear after cleaning; the water flow test is specifically: connect the cleaned suction filter head to an appropriate water source and observe the flow of water through the suction filter head. If the water flow is smooth, without obvious blockage or slowdown, then it can be preliminarily judged that the suction filter head has been cleaned; the weight comparison is specifically: weigh the suction filter head before and after cleaning. If the weight of the suction filter head is significantly reduced after cleaning, then it can be considered that most of the contaminants on the suction filter head have been removed.

[0059] The present application will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are intended only to illustrate the present application and are not intended to limit the scope of the present application. The experimental methods for which specific conditions are not specified in the following examples are usually measured according to industry standards. If there is no corresponding industry standard, then the conditions recommended by the manufacturer are followed.

[0060] Example 1

[0061] This embodiment provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising the following steps:

[0062] S11. The weight of the liquid chromatography mobile phase suction filter to be cleaned is 7.5835 g. There are a small amount of particulate matter on the surface of the suction filter and the color changes slightly, showing light green, but the overall appearance is relatively clean, and the suction filter shows signs of a slight decrease in water flow rate. It is thus determined that the degree of contamination of the cleaned liquid chromatography mobile phase suction filter is slightly contaminated. Put the liquid chromatography mobile phase suction filter to be cleaned into a beaker, clean the suction filter with pure water or deionized water, and rotate the filter to make the water cover all pores as much as possible to remove dirt and residues on the surface to obtain the first suction filter;

[0063] S21, place the first suction filter head in a solution containing 75% ethanol, soak for 5 minutes, then use a soft brush or cotton swab to dip in an appropriate amount of alcohol, gently scrub the surface and pores of the suction filter head to ensure that the microorganisms are completely removed, and obtain a second suction filter head;

[0064] S31, put the second suction filter head into the diluted 5% dilute nitric acid, and ensure that the suction filter head is completely immersed in the solution. Use a stirring rod to gently stir the solution so that the suction filter head is fully in contact with the dilute nitric acid. The immersion time is 35 minutes, and a third suction filter head is obtained;

[0065] S41. After soaking, rinse the suction filter head thoroughly with deionized water to ensure that all residual nitric acid is removed. Check that the suction filter head has been cleaned. Finally, place the suction filter head in a dry and ventilated place, let it dry naturally or use a hair dryer to gently blow dry to obtain a clean liquid chromatography mobile phase suction filter head.

[0066] The weight of the cleaned liquid chromatography mobile phase suction filter head is 7.5277g. The weight of the suction filter head after cleaning is significantly reduced compared with that before cleaning. After cleaning, the particles and color changes on the surface of the suction filter head have disappeared. Under the same test conditions, the suction filter head after cleaning shows that the water flow rate has returned to normal. This shows that the blockage inside the suction filter head has been effectively removed and the filtration performance has been restored.

[0067] Example 2

[0068] This embodiment provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising the following steps:

[0069] S11. The weight of the liquid chromatography mobile phase suction filter to be cleaned is 7.6614 g. The visual inspection results show that there are obvious particles and color changes on the surface of the suction filter, green microorganisms are attached, and there may be some small blockages. The water flow test results show that the suction filter shows a slow decrease in water flow rate. It is thus determined that the degree of contamination of the cleaned liquid chromatography mobile phase suction filter is moderately contaminated. Put the liquid chromatography mobile phase suction filter to be cleaned into a beaker, clean the suction filter with pure water or deionized water, rotate the filter head so that the water covers all pores as much as possible to remove surface dirt and residues, and obtain the first suction filter head;

[0070] S21, placing the first suction filter head in a solution containing 75% ethanol, soaking for 8 minutes, then using a soft brush or cotton swab dipped in an appropriate amount of alcohol, gently scrubbing the surface and pores of the suction filter head to ensure that the microorganisms are completely removed, and obtaining a second suction filter head;

[0071] S31, put the second suction filter head into the diluted 5% dilute nitric acid, and ensure that the suction filter head is completely immersed in the solution. Use a stirring rod to gently stir the solution so that the suction filter head is fully in contact with the dilute nitric acid. The soaking time is 45 minutes, and a third suction filter head is obtained;

[0072] S41. After soaking, rinse the suction filter head thoroughly with deionized water to ensure that all residual nitric acid is removed. Check that the suction filter head has been cleaned. Finally, place the suction filter head in a dry and ventilated place, let it dry naturally or use a hair dryer to gently blow dry to obtain a clean liquid chromatography mobile phase suction filter head.

[0073] The weight of the cleaned liquid chromatography mobile phase suction filter head is 7.5356g. The weight of the suction filter head after cleaning is significantly reduced compared with that before cleaning. After cleaning, the particles and color changes on the surface of the suction filter head have been significantly reduced and disappeared. In addition, the blockage has been effectively removed, and the suction filter head has a cleaner and more transparent appearance. Under the same test conditions, the suction filter head after cleaning shows that the water flow rate has returned to normal. This shows that the blockage inside the suction filter head has been effectively removed and the filtration performance has been restored.

[0074] Example 3

[0075] This embodiment provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising the following steps:

[0076] S11. The weight of the liquid chromatography mobile phase suction filter to be cleaned is 7.7845 g. The visual inspection results show that there are a large number of particulate matter and obvious discoloration on the surface of the suction filter, a large number of green microorganisms attached to the surface of the suction filter, and serious blockages. The water flow test results show that the suction filter shows a significant decrease in water flow rate, and the water drips in drops. It is thus determined that the degree of contamination of the cleaned liquid chromatography mobile phase suction filter is severely contaminated. Put the liquid chromatography mobile phase suction filter to be cleaned into a beaker, clean the suction filter with pure water or deionized water, rotate the filter head so that the water covers all pores as much as possible to remove surface dirt and residues, and obtain the first suction filter head;

[0077] S21, placing the first suction filter head in a solution containing 75% ethanol, soaking for 10 minutes, then using a soft brush or cotton swab dipped in an appropriate amount of alcohol, gently scrubbing the surface and pores of the suction filter head to ensure that the microorganisms are completely removed, and obtaining a second suction filter head;

[0078] S31, put the second suction filter head into the diluted 5% dilute nitric acid, and ensure that the suction filter head is completely immersed in the solution. Use a stirring rod to gently stir the solution so that the suction filter head is fully in contact with the dilute nitric acid. The soaking time is 55 minutes, and a third suction filter head is obtained;

[0079] S41. After soaking, rinse the suction filter head thoroughly with deionized water to ensure that all residual nitric acid is removed. Check that the suction filter head has been cleaned. Finally, place the suction filter head in a dry and ventilated place, let it dry naturally or use a hair dryer to gently blow dry to obtain a clean liquid chromatography mobile phase suction filter head.

[0080] The weight of the cleaned liquid chromatography mobile phase suction filter head is 7.5714g. The weight of the suction filter head after cleaning is significantly reduced compared with that before cleaning. This shows that some pollutants have been successfully removed during the cleaning process. After cleaning, the particles and color changes on the surface of the suction filter head have disappeared. In addition, the blockage has been effectively removed, and the suction filter head has a cleaner and more transparent appearance. Under the same test conditions, the suction filter head after cleaning shows that the water flow rate has returned to normal. This shows that the blockage inside the suction filter head has been effectively removed and the filtration performance has been restored.

[0081] Comparative Example 1

[0082] This comparative example provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising the following steps:

[0083] S11. The weight of the liquid chromatography mobile phase suction filter to be cleaned before cleaning is 7.5857g. Visual inspection: There are obvious particles and color changes on the surface of the suction filter, green microorganisms are attached, and there are some small blockages. Water flow test and other results: The suction filter shows that the water flow rate decreases slowly. It is thus determined that the degree of contamination of the cleaned liquid chromatography mobile phase suction filter is moderately contaminated. Place the liquid chromatography mobile phase suction filter to be cleaned in a beaker, clean the suction filter with pure water or deionized water, rotate the filter head so that the water covers all pores as much as possible to remove dirt and residues on the surface, and obtain the first suction filter head;

[0084] S21, put the first suction filter head into the diluted 5% dilute nitric acid, and ensure that the suction filter head is completely immersed in the solution. Use a stirring rod to gently stir the solution so that the suction filter head is fully in contact with the dilute nitric acid. The immersion time is 45 minutes, and a second suction filter head is obtained;

[0085] S31. After soaking, rinse the suction filter head thoroughly with deionized water to ensure that all residual nitric acid is removed. Finally, place the suction filter head in a dry and ventilated place, let it dry naturally or use a hair dryer to gently blow dry to obtain a clean liquid chromatography mobile phase suction filter head.

[0086] The weight of the cleaned liquid chromatography mobile phase suction filter head is 7.5538g. The weight of the suction filter head after cleaning is slightly lighter than that before cleaning. After cleaning, the particles on the surface of the suction filter head disappeared, the color change decreased but did not disappear, and some green microorganisms could not be completely cleaned. Under the same test conditions, the suction filter head after cleaning showed that the water flow rate was restored, but not completely restored, and there was still some blockage. This shows that the blockage inside the suction filter head has not been completely removed and the filtration performance has not been fully restored.

[0087] Comparative Example 2

[0088] This comparative example provides a method for cleaning a liquid chromatography mobile phase suction filter head, comprising the following steps:

[0089] S11. The weight of the liquid chromatography mobile phase suction filter to be cleaned is 7.6257 g. Visual inspection: There are obvious particles and color changes on the surface of the suction filter, green microorganisms are attached, and there are some small blockages. Water flow test and other results: The suction filter shows that the water flow rate decreases slowly. It is thus determined that the degree of contamination of the cleaned liquid chromatography mobile phase suction filter is moderately contaminated. Place the liquid chromatography mobile phase suction filter to be cleaned in a beaker, clean the suction filter with pure water or deionized water, rotate the filter so that the water covers all pores as much as possible to remove dirt and residues on the surface, and obtain the first suction filter;

[0090] S21, placing the first suction filter head in a solution containing 75% ethanol, soaking for 10 minutes, then using a soft brush or cotton swab dipped in an appropriate amount of alcohol, gently scrubbing the surface and pores of the suction filter head to ensure that the microorganisms are completely removed, and obtaining a second suction filter head;

[0091] S31. After soaking, rinse the suction filter head thoroughly with deionized water. Finally, place the suction filter head in a dry and ventilated place, let it dry naturally or use a hair dryer to gently blow dry it to obtain a clean liquid chromatography mobile phase suction filter head.

[0092] The weight of the cleaned liquid chromatography mobile phase suction filter head is 7.5421g. The weight of the suction filter head after cleaning is slightly lighter than that before cleaning. After cleaning, the particles on the surface of the suction filter head are reduced but not completely eliminated, and the color change has disappeared. Under the same test conditions, the suction filter head after cleaning shows that the water flow rate has recovered, but not completely, and there is still some blockage. This indicates that the blockage inside the suction filter head has not been completely removed and the filtration performance has not been fully restored.

[0093] It can be seen from the embodiments and comparative examples that embodiments 1 to 3 use 75% ethanol and 5% nitric acid to clean the liquid phase suction filter head. Through the synergistic treatment of the ethanol solution and the dilute nitric acid, bacteria, molds and other microorganisms, impurities and residues attached to the surface of the suction filter head can be efficiently removed. Comparative Example 1 does not use ethanol solution for cleaning, and Comparative Example 2 does not use dilute nitric acid for cleaning. Bacteria, molds and other microorganisms, impurities and residues attached to the surface of the suction filter head are not completely removed, and the suction filter head after cleaning does not meet the subsequent use requirements.

[0094] In addition, one or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:

[0095] In the embodiments of the present invention, the provided method can improve the accuracy and reliability of the experiment, extend the service life of the suction filter, protect the experimental instrument, improve the experimental efficiency, and is easy to operate and environmentally friendly, ensuring the operation cycle of the liquid chromatograph, and has good promotion and application value.

[0096] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novel features applied for herein.

Claims

1. A method for cleaning a liquid chromatography mobile phase suction filter head, characterized in that: The method comprises: Preliminarily cleaning the liquid chromatography mobile phase suction filter to be cleaned to obtain a first suction filter; soaking the first suction filter head in an ethanol solution to obtain a second suction filter head; The second suction filter head is immersed in a dilute acid solution for a second time, and the time of the second immersion is determined according to the contamination degree of the liquid chromatography mobile phase suction filter head to be cleaned, to obtain a third suction filter head; The third suction filter is post-processed to obtain a clean liquid chromatography mobile phase suction filter.

2. The method according to claim 1, characterized in that The step of determining the second soaking time according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned comprises: The second soaking time is determined according to the contamination degree of the liquid chromatography mobile phase suction filter to be cleaned; wherein, If the degree of contamination is mild, the soaking time is 30 to 40 minutes; If the degree of contamination is moderate, the soaking time is 40 to 50 minutes; If the pollution degree is severe, the soaking time is 50 minutes to 60 minutes.

3. The method according to claim 2, characterized in that The contamination degree is determined based on the stains, residues, color changes and blockages of the liquid chromatography mobile phase suction filter to be cleaned.

4. The method according to claim 1, characterized in that: The dilute acid solution is dilute nitric acid, and the volume concentration of the dilute nitric acid is 1% to 10%.

5. The method according to claim 1, characterized in that After the first suction filter head is first immersed in the ethanol solution, the method further comprises: Use a soft brush or cotton swab to dip in an appropriate amount of ethanol solution to scrub the surface and pores of the first suction filter head after the first soaking to remove microorganisms on the surface of the first suction filter head.

6. The method according to claim 5, characterized in that The first soaking time is 5 min to 10 min.

7. The method according to claim 5, characterized in that The volume concentration of the ethanol solution is 60% to 80%.

8. The method according to claim 1, characterized in that The method of preliminarily cleaning the liquid chromatography mobile phase suction filter to be cleaned to obtain a first suction filter comprises: The liquid chromatography mobile phase suction filter to be cleaned is placed in a container, the suction filter is cleaned with pure water or deionized water, and the suction filter is rotated to allow the water to cover the pores to remove dirt and residues on the surface of the suction filter, thereby obtaining a first suction filter.

9. The method according to claim 1, characterized in that: The third suction filter head is post-processed to obtain a clean liquid chromatography mobile phase suction filter head, comprising: Rinse the third suction filter with deionized water; Checking the cleanliness of the third suction filter after flushing; If the third suction filter does not meet the cleaning requirements, repeat the cleaning process; If the third suction filter meets the cleaning requirement, the third suction filter is dried to obtain a clean liquid chromatography mobile phase suction filter.

10. The method according to claim 9, characterized in that The inspection method includes one of the following: visual inspection, water flow test, and weight comparison.

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