Umbilical cord blood hematopoietic stem cell extraction and separation device

Abstract

The invention relates to the technical field of stem cell separation, and provides an umbilical cord blood hematopoietic stem cell extraction and separation device which comprises an extraction device body and a top plate, the top plate is installed at the top of the extraction device body, a pressing device is installed in the middle of the bottom of the top plate, and an umbilical cord placing plate is installed on the upper portion of the inner wall of the extraction device body. The middle of the umbilical cord placing plate penetrates through and is fixedly connected with a crushing net, the crushing net is matched with a pressing device, the upper portion and the lower portion of the middle of the inner wall of the extraction device body are each fixedly connected with two stem cell interception plates, and the middles of the two stem cell interception plates are each fixedly connected with a stem cell extraction plate. By means of the technical scheme, the problems that in the prior art, separated stem cells need to be taken out after stem cell separation is completed, and the operation that the stem cells on the filter screen need to be taken out manually in cooperation with a tool is tedious are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell separation, and in particular to a device for extracting and separating umbilical cord blood hematopoietic stem cells. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells; according to the developmental potential of stem cells, they can be divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are a type of undifferentiated and immature cell that has the potential to regenerate various organs and the human body. The medical community calls them universal cells. Current research has found that mesenchymal stem cells can be isolated and cultured from umbilical cord blood, and mesenchymal stem cells have been isolated and cultured from the umbilical vein endothelium and subendothelium, umbilical cord jelly and perivascular tissue.

[0003] In a common umbilical cord blood hematopoietic stem cell extraction and separation device, the umbilical cord is first flushed to remove impurities, then the umbilical cord is crushed by squeezing the umbilical cord, and then the squeezed and crushed umbilical cord is gradually separated through a separation filter to decompose and extract the stem cells in the umbilical cord. However, after the stem cell separation is completed, the separated stem cells need to be removed. Usually, the stem cells on the filter are removed by special tools, which not only requires manual cooperation, but also is cumbersome to operate and has low work efficiency. Secondly, the filter needs to be cleaned after removal, but there will still be a large amount of residue on the inner wall of the device. In some common devices, the separation filter can be cleaned with clean water, but the inner wall of the device cannot be thoroughly flushed, and some cell fragments and other residues will remain, which will cause cross contamination to the next use and affect subsequent use. Summary of the invention

[0004] The purpose of the present invention is to provide a device for extracting and separating hematopoietic stem cells from umbilical cord blood to solve the problems raised in the above-mentioned background technology.

[0005] The technical solution of the present invention is as follows: In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions: an umbilical cord blood hematopoietic stem cell extraction and separation device, comprising an extraction device body and a top plate, wherein the top plate is installed on the top of the extraction device body, a down-pressor is installed in the middle of the bottom of the top plate, an umbilical cord placement plate is installed on the upper part of the inner wall of the extraction device body, a crushing net is passed through and fixedly connected to the middle of the umbilical cord placement plate, and the crushing net cooperates with the down-pressor, two stem cell retention plates are fixedly connected to the upper and lower parts of the middle of the inner wall of the extraction device body, and the middle parts of the two stem cell retention plates are fixedly connected to the stem cell extraction plate, a plasma retention plate is installed at the bottom of the inner wall of the extraction device body, a material discharge assembly is installed inside the extraction device body, and a cleaning assembly is arranged on the right part of the extraction device body; The unloading assembly includes two sets of gear rings 1 and stem cell push plates. The two sets of gear rings 1 are rotatably connected to the middle of the two stem cell retention plates. The stem cell push plates are fixedly connected to one side of the inner wall of gear ring 1. The bottom of the stem cell push plates is attached to the top of the stem cell extraction plate. One side of the stem cell retention plate is penetrated and provided with a stem cell discharge port. The stem cell discharge port cooperates with the stem cell push plates. A gear groove is provided on one side of the interior of the stem cell retention plate. The middle of the plasma retention plate is rotatably connected with gear ring 2. One side of the inner wall of gear ring 2 is fixedly connected with a plasma push plate. One side of the plasma push plates is penetrated and provided with a plasma discharge port. The bottom of the extraction device body is fixedly connected with output box 1. The bottom of the output box 1 is fixedly connected with output box 2. A motor is installed at the bottom of the output box 2. The output end of the motor penetrates the output box 2 and is fixedly connected A rotating shaft is connected, and a gear 1 is fixedly connected to the middle part of the outer wall of the rotating shaft, and the gear 1 rotates inside the output box 2. An output groove is opened on one side of the inner part of the output box 1. A synchronous wheel 1 is fixedly connected to the top of the outer wall of the rotating shaft, and a rotating rod is penetrated and rotatably connected to one side of the output box 1. The rotating rod penetrates the extraction device body, the plasma retention plate and the stem cell retention plate and is rotatably connected. A synchronous wheel 2 is fixedly connected to the bottom of the outer wall of the rotating rod, and the synchronous wheel 2 is located in the output groove. A synchronous belt is sleeved on the outer wall of the synchronous wheel 2, and the other end of the synchronous belt is sleeved on the synchronous wheel 1. The rotating rod is located at the outer wall that penetrates the plasma retention plate and is fixedly connected with the gear 2, and the gear 2 is meshed with the gear ring 2. The side of the rotating rod located inside the stem cell retention plate is fixedly connected with two gears 3, and the gear 3 rotates in the gear groove and is meshed with the gear ring 1.

[0006] Preferably, the cleaning assembly includes a water tank, a water pump and a water pipe 1, the water tank is arranged on the right part of the extraction device body, the water pump is installed on the lower part of the water tank close to the extraction device body, the water pipe 1 is fixedly connected to the output end of the water pump, the end of the water pipe 1 away from the water pump passes through the output box 2, a water groove is provided on one side of the interior of the output box 2, the water groove cooperates with the output box 2, a sleeve is rotatably connected to the top of the water groove, the sleeve passes through the output box 2, the output box 1 is fixedly connected to the extraction device body, a guide block is fixedly connected to one side of the inner wall of the sleeve, and the inner rotation connection of the sleeve A water pipe 2 is connected, and a guide groove is provided on the inner outer wall of the sleeve, and the guide groove cooperates with the guide block. A gear 4 is fixedly connected to the bottom of the outer wall of the sleeve, and the gear 4 is meshed with the gear 1. The outer wall tube of the water pipe 2 passes through the stem cell retention plate and is rotatably connected thereto. Three angled nozzles are fixedly connected to the outer wall of the water pipe 2, and a right-angled nozzle is fixedly connected to the side of the outer wall of the water pipe 2 away from the angled nozzle. The angled nozzle cooperates with the stem cell retention plate and the plasma retention plate, and the right-angled nozzle cooperates with the inner wall of the extraction device body. A water inlet is provided on the top of the water tank.

[0007] Preferably, the top of the outer wall of the water pipe one is penetrated and fixedly connected with the water pipe three, the top of the water pipe three is fixedly connected with an annular tube, the annular tube is sleeved on the upper part of the outer wall of the extraction device body, the inner wall of the annular tube is fixedly connected with a nozzle, the nozzle penetrates the extraction device body and cooperates with the crushing net.

[0008] Preferably, a plasma collection box is fixedly connected to one side of the outer wall of the extraction device body, and the plasma collection box cooperates with the plasma discharge port.

[0009] Preferably, two stem cell collection boxes are provided on one side of the outer wall of the extraction device body, located above the plasma collection box, and the stem cell collection box is connected to the stem cell discharge port. The plasma collection box and the stem cell collection box are both provided with a material collection port on the side away from the extraction device body.

[0010] Preferably, a loading door is provided on the upper part of the outer wall of the extraction device body away from the water tank, a stem cell inspection door is provided on the upper and middle parts of the outer wall of the extraction device body away from the water tank, and a plasma inspection door is provided on the lower part of the outer wall of the extraction device body away from the water tank.

[0011] Preferably, the two stem cell retention plates are both installed obliquely in the extraction device body to promote material flow during material discharge and cleaning.

[0012] Preferably, the inner wall of the plasma retention plate is arranged in an inclined state, so that the plasma and impurities inside can be cleaned more thoroughly during unloading and cleaning.

[0013] Preferably, the stem cell retention plate is divided into upper and lower sides, the stem cell extraction plate inside the upper stem cell retention plate can retain stem cells, and the stem cell extraction plate inside the lower stem cell retention plate can retain white blood cells.

[0014] Preferably, the four bottom corners of the extraction device body are fixedly connected with supporting feet.

[0015] The working principle and beneficial effects of the present invention are: 1. In the present invention, the motor is turned on to make the rotating shaft drive the rotating rod to rotate. When the rotating rod rotates, gear 2 meshes with gear ring 2, so that the plasma pushing plate swings to push the plasma in the plasma retention plate to the plasma discharge port for discharge, and enters the plasma collection box through the plasma discharge port to collect the plasma. At the same time, when gear 3 rotates, it meshes with gear ring 1, so that the stem cell pushing plate rotates to push the stem cells and white blood cells on the stem cell extraction plate to the stem cell discharge port for discharge, and the stem cell discharge port is connected with the stem cell collection box to discharge the stem cells and white blood cells into the water tank for collection. When the collection is completed, the material taking port is opened to take out the separated stem cells, white blood cells and plasma, which solves the problem that the separated stem cells need to be taken out after the stem cell separation is completed, and the stem cells on the filter need to be taken out manually with tools, which is a cumbersome operation.

[0016] 2. In the present invention, the water pump is turned on to extract water from the water tank. The water flow will first enter the water trough, and then enter the water pipe 2 through the pressure of the water pump. The inside of the stem cell retention plate and the inside of the plasma retention plate are rinsed through the oblique angle nozzle, and the inner wall of the extraction device body is rinsed through the right-angle nozzle. During the rinsing process, the motor is turned on to drive the rotating shaft to rotate so that the gear one and the gear four are engaged to drive the sleeve to rotate. When the sleeve rotates, the guide block rotates in the guide groove to drive the water pipe 2 to rotate and move upward at the same time. While the water pipe 2 moves and rotates upward, the inner wall of the plasma retention plate and the inner wall of the stem cell retention plate can be fully rinsed through the oblique angle nozzle. At the same time, the plasma push plate and the stem cell push plate will also swing to scrape the top of the plasma retention plate and the top of the stem cell extraction plate. The inner wall of the extraction device body is rinsed by the right-angle nozzle as it rotates upward to avoid residue on the inner wall of the extraction device body, so that the cleaning is more comprehensive. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a cross-sectional view of the extraction device body of the present invention; Figure 3 This is a schematic diagram of a stem cell collection box of the present invention; Figure 4 It is a schematic diagram of the umbilical cord placement board of the present invention; Figure 5 is a cross-sectional view of a stem cell retention plate of the present invention; Figure 6 It is a schematic diagram of the plasma retention plate of the present invention; Figure 7 It is a cross-sectional view of the output box 1 and the output box 2 of the present invention; Figure 8 It is a schematic diagram of a water pump of the present invention.

[0018] In the figure: 1. Extraction device body; 2. Top plate; 3. Downcomer; 4. Umbilical cord placement plate; 5. Crushing net; 6. Stem cell retention plate; 7. Stem cell extraction plate; 8. Gear ring 1; 9. Stem cell push plate; 10. Stem cell discharge port; 11. Gear slot; 12. Output box 1; 13. Output box 2; 14. Motor; 15. Rotating shaft; 16. Gear 1; 17. Output slot; 18. Synchronous wheel 1; 19. Rotating rod; 20. Synchronous wheel 2; 21. Synchronous belt; 22. Gear 2; 23. Gear 3; 24. Plasma retention plate; 25. Gear ring 2; 26. Plasma push plate; 27. Plasma outlet; 28. Plasma collection box; 29. ​​Stem cell collection box; 30. Water tank; 31. Water pump; 32. Water pipe 1; 33. Water trough; 34. Casing; 35. Guide block; 36. Water pipe 2; 37. Guide groove; 38. Gear 4; 39. Angled nozzle; 40. Right-angle nozzle; 41. Water pipe 3; 42. Annular pipe; 43. Nozzle; 44. Water inlet; 45. Loading door; 46. Stem cell inspection door; 47. Plasma inspection door; 48. Material extraction port; 49. Support foot. DETAILED DESCRIPTION

[0019] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] like Figures 1 to 8As shown, an embodiment of the present invention provides an umbilical cord blood hematopoietic stem cell extraction and separation device, comprising an extraction device body 1, a top plate 2, the top plate 2 is installed on the top of the extraction device body 1, a down-pressor 3 is installed in the middle of the bottom of the top plate 2, an umbilical cord placement plate 4 is installed on the upper part of the inner wall of the extraction device body 1, a crushing net 5 is penetrated through and fixedly connected to the middle of the umbilical cord placement plate 4, the crushing net 5 cooperates with the down-pressor 3, two stem cell retention plates 6 are fixedly connected to the upper and lower parts of the middle of the inner wall of the extraction device body 1, the two stem cell retention plates 6 are obliquely installed in the extraction device body 1, and promote material flow during material discharge and cleaning, the middle of the two stem cell retention plates 6 are fixedly connected to a stem cell extraction plate 7, a plasma retention plate 24 is installed at the bottom of the inner wall of the extraction device body 1, and the inner wall of the plasma retention plate 24 It is set to an inclined state to ensure that the plasma and impurities inside are cleaned more thoroughly during unloading and cleaning. A unloading component is installed inside the extraction device body 1, and a cleaning component is installed on the right side of the extraction device body 1. The plasma will flow into the plasma retention plate 24 for retention. The four corners of the bottom of the extraction device body 1 are fixedly connected with support feet 49 for supporting the extraction device body 1 and leaving space for the installation of the output box 1 12, the output box 2 13 and the motor. The two stem cell retention plates 6 are both installed obliquely in the extraction device body 1 to promote the flow of materials during unloading and cleaning. The stem cell retention plate 6 is divided into an upper and lower side. The stem cell extraction plate 7 inside the upper stem cell retention plate 6 can retain stem cells, and the stem cell extraction plate 7 inside the lower stem cell retention plate 6 can retain white blood cells. The material discharge assembly includes two sets of toothed rings 8 and stem cell push plates 9. The two sets of toothed rings 8 are rotatably connected to the middle of the two stem cell retention plates 6. The stem cell push plate 9 is fixedly connected to one side of the inner wall of the toothed ring 8. The bottom of the stem cell push plate 9 is attached to the top of the stem cell extraction plate 7. One side of the stem cell retention plate 6 is penetrated and provided with a stem cell discharge port 10. The stem cell discharge port 10 cooperates with the stem cell push plate 9. A gear groove 11 is provided on one side of the interior of the stem cell retention plate 6. The middle part of the plasma retention plate 24 is rotatably connected to a toothed ring 25. One side of the inner wall of the toothed ring 25 is fixedly connected. There is a plasma push plate 26, one side of the plasma push plate 26 penetrates and is provided with a plasma discharge port 27, the bottom of the extraction device body 1 is fixedly connected to an output box 12, the bottom of the output box 12 is fixedly connected to an output box 2 13, a motor 14 is installed at the bottom of the output box 2 13, the output end of the motor 14 penetrates the output box 2 13 and is fixedly connected to a rotating shaft 15, a gear 16 is fixedly connected to the middle of the outer wall of the rotating shaft 15, the gear 16 rotates inside the output box 2 13, an output slot 17 is provided on one side of the inner side of the output box 12, and the top of the outer wall of the rotating shaft 15 is fixedly connected to There is a synchronous wheel 18, one side of the output box 12 penetrates and is rotatably connected with a rotating rod 19, and the rotating rod 19 penetrates the extraction device body 1, the plasma retention plate 24 and the stem cell retention plate 6 and is rotatably connected, the outer wall bottom of the rotating rod 19 is fixedly connected with a synchronous wheel 20, the synchronous wheel 20 is located in the output groove 17, the outer wall of the synchronous wheel 20 is sleeved with a synchronous belt 21, and the other end of the synchronous belt 21 is sleeved on the synchronous wheel 18, the rotating rod 19 is located on the outer wall of the plasma retention plate 24 and is fixedly connected with a gear 22, the gear 22 is meshed with the gear ring 25, and the rotating rod 19 The side portion located inside the stem cell retention plate 6 is fixedly connected with two gear threes 23, which rotate in the gear groove 11 and mesh with the gear ring 1 8. At the same time, when the gear three 23 rotates, it meshes with the gear ring 1 8, so that the stem cell push plate 9 rotates to push the stem cells and white blood cells on the stem cell extraction plate 7 to the stem cell discharge port 10 for discharge. The stem cell discharge port 10 is connected with the stem cell collection box 29 to discharge the stem cells and white blood cells into the water tank 30 for collection. When the collection is completed, the material taking port 48 is opened to take out the separated stem cells, white blood cells and plasma.

[0021] Wherein, the cleaning component includes a water tank 30, a water pump 31 and a water pipe 1 32. The water tank 30 is arranged on the right part of the extraction device body 1, and the water pump 31 is installed on the lower part of the side of the water tank 30 close to the extraction device body 1. The water pipe 1 32 is fixedly connected to the output end of the water pump 31, and the end of the water pipe 1 32 away from the water pump 31 passes through the output box 2 13, and a water groove 33 is provided on one side of the inner part of the output box 2 13. The water groove 33 cooperates with the output box 2 13, and the top of the water groove 33 is rotatably connected with a sleeve 34, which passes through the output box 2 13, the output box 1 12 and is fixedly connected to the extraction device body 1, and a guide block 35 is fixedly connected to one side of the inner wall of the sleeve 34, and the inner part of the sleeve 34 is rotatably connected with a water Pipe 2 36, start the water pump 31 to extract water from the water tank 30, the water flow will first enter the water trough 33, and then enter the water pipe 2 36 through the pressure of the water pump. The water pipe 2 36 is located on the inner outer wall of the sleeve 34 and has a guide groove 37. The guide groove 37 cooperates with the guide block 35. The outer wall bottom of the sleeve 34 is fixedly connected with a gear 4 38, and the gear 4 38 is meshed with the gear 1 16. The outer wall tube of the water pipe 2 36 passes through the stem cell retention plate 6 and is rotatably connected thereto. During the flushing process, the motor 14 is turned on to drive the rotating shaft 15 to rotate so that the gear 1 16 is meshed with the gear 4 38 to drive the sleeve 34 to rotate. When the sleeve 34 rotates, it will rotate in the guide groove 37 through the guide block 35 to drive the water pipe 2 36 rotates and moves upward at the same time. When the water pipe 36 moves upward and rotates, the inner wall of the plasma retention plate 24 and the inner wall of the stem cell retention plate 6 can be fully flushed through the angled nozzle 39. The outer wall of the water pipe 36 is fixedly connected with three angled nozzles 39. The outer wall of the water pipe 36 is away from the angled nozzle 39 and the right-angled nozzle 40 is fixedly connected. The angled nozzle 39 cooperates with the stem cell retention plate 6 and the plasma retention plate 24. The right-angled nozzle 40 cooperates with the inner wall of the extraction device body 1. The top of the water tank 30 is provided with a water inlet 44. The inside of the stem cell retention plate 6 and the inside of the plasma retention plate 24 are flushed through the angled nozzle 39, and the extraction device is flushed through the right-angled nozzle 40. The inner wall of the main body 1 is rinsed, and at the same time, the plasma pushing plate 26 and the stem cell pushing plate 9 will also swing to scrape the top of the plasma retention plate 24 and the top of the stem cell extraction plate 7, so that the cleaning is more comprehensive. The top of the outer wall of the water pipe 32 is penetrated and fixedly connected with a water pipe 3 41, and the top of the water pipe 3 41 is fixedly connected with an annular tube 42, which is sleeved on the upper part of the outer wall of the extraction device body 1, and the inner wall of the annular tube 42 is fixedly connected with a nozzle 43, which penetrates the extraction device body 1 and cooperates with the crushing net 5. When the water pump 31 is turned on, the water flow will also enter the water pipe 3 41, and be transmitted to the annular tube 42 and the nozzle 43 through the water pipe 3 41 to rinse the down-pressor 3 and the umbilical cord placement plate 4.

[0022] Among them, a plasma collection box 28 is fixedly connected to one side of the outer wall of the extraction device body 1, and the plasma collection box 28 cooperates with the plasma discharge port 27. The plasma enters the plasma collection box 28 through the plasma discharge port 27 to collect the plasma. Two stem cell collection boxes 29 are opened on one side of the outer wall of the extraction device body 1 at the upper part of the plasma collection box 28. The stem cell collection box 29 is connected to the stem cell discharge port 10. Through the connection between the stem cell discharge port 10 and the stem cell collection box 29, the stem cells and white blood cells are discharged into the water tank 30 for collection. The plasma collection box 28 and the stem cell collection box 29 are both provided with a material taking port 48 on the side away from the extraction device body 1. When the collection is completed, the material taking port 48 is opened to take out the separated stem cells, white blood cells and plasma.

[0023] Among them, a loading door 45 is provided on the upper part of the outer wall of the extraction device body 1 away from the water tank 30. The loading door 45 is opened to place the umbilical cord on the crushing net 5. Stem cell inspection doors 46 are provided on the upper and middle parts of the outer wall of the extraction device body 1 away from the water tank 30. A plasma inspection door 47 is provided on the lower part of the outer wall of the extraction device body 1 away from the water tank 30. The stem cell inspection door 46 and the plasma inspection door 47 are convenient for technicians to carry out equipment maintenance and ensure the normal operation of the equipment.

[0024] Working principle: During operation, first open the loading door 45 to place the umbilical cord on the crushing net 5, start the water pump 31 to clean the umbilical cord placed on the crushing net 5 through the water pipe 3 41 and the ring pipe 42 nozzle 43, and at the same time open the material taking port 48 to discharge the sewage after cleaning the umbilical cord through the plasma collection box 28 and the stem cell collection box 29, then open the down-pressor 3 to press down to crush the umbilical cord, and with the influence of gravity, the umbilical cord blood will pass through the two stem cell retention plates 6 in turn, pass through the stem cell extraction plate 7 inside the first layer of stem cell retention plate 6 to retain the stem cells, and then pass through the stem cell extraction plate 7 inside the second layer of stem cell retention plate 6 to retain the white blood cells, and then the remaining plasma will flow into the plasma retention plate 24, and after the separation is completed, start the motor 14 to drive the rotating shaft 15 to drive The synchronous wheel 18 rotates, and at the same time, the rotating rod 19 rotates through the synchronous belt 21 and the gear 2 22. When the rotating rod 19 rotates, the gear 2 22 and the gear 3 23 are driven to rotate. The gear 2 22 meshes with the gear ring 2 25, so that the plasma push plate 26 swings to push the plasma in the plasma retention plate 24 to the plasma discharge port 27 for discharge, and enters the plasma collection box 28 through the plasma discharge port 27 for collection. At the same time, when the gear 3 23 rotates, it meshes with the gear ring 1 8, so that the stem cell push plate 9 rotates to push the stem cells and white blood cells on the stem cell extraction plate 7 to the stem cell discharge port 10 for discharge, and the stem cell discharge port 10 is connected with the stem cell collection box 29 to discharge the stem cells and white blood cells into the water tank 30 for collection. When the collection is completed, the material taking port 48 is opened to take out the separated stem cells, white blood cells and plasma; When the separation and extraction is completed, the water pump 31 is turned on to extract the water in the water tank 30. The water flow will first enter the water trough 33, and then enter the water pipe 2 36 through the pressure of the water pump. The inside of the stem cell retention plate 6 and the inside of the plasma retention plate 24 are rinsed through the angled nozzle 39, and the inner wall of the extraction device body 1 is rinsed through the right-angle nozzle 40. During the rinsing process, the motor 14 is turned on to drive the rotating shaft 15 to rotate so that the gear 1 16 and the gear 4 38 are engaged to drive the sleeve 34 to rotate. When the sleeve 34 rotates, it will rotate in the guide groove 37 through the guide block 35 to drive the water pipe 2 36 to rotate and move upward at the same time. While the water pipe 2 36 moves and rotates upward, the inner wall of the plasma retention plate 24 and the inner wall of the stem cell retention plate 6 can be omnidirectionally sprayed through the angled nozzle 39. At the same time, the plasma push plate 26 and the stem cell push plate 9 will also swing to scrape the top of the plasma retention plate 24 and the top of the stem cell extraction plate 7, so that the cleaning is more comprehensive. The inner wall of the extraction device body 1 is rinsed by the right-angle nozzle 40 as it rotates upward to avoid residue on the inner wall of the extraction device body 1. When the water pump 31 is turned on, the water flow will also enter the water pipe three 41, and be transmitted to the annular pipe 42 and the nozzle 43 through the water pipe three 41 to rinse the down-pressor 3 and the umbilical cord placement plate 4. During the flushing, the water flow will pass through the plasma collection box 28 and the stem cell collection box 29 to clean the inside of the plasma collection box 28 and the stem cell collection box 29, and then the cleaned sewage will be discharged through the material taking port 48. After the work is completed, the motor 14 is turned over to reset.

[0025] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cord blood hematopoietic stem cell extraction and separation device, comprising an extraction device body (1), a top plate (2), wherein the top plate (2) is mounted on the top of the extraction device body (1), and a presser (3) is mounted in the middle of the bottom of the top plate (2), characterized in that: An umbilical cord placement plate (4) is installed on the upper part of the inner wall of the extraction device body (1); a crushing net (5) is passed through and fixedly connected to the middle part of the umbilical cord placement plate (4); the crushing net (5) cooperates with the lower pressure device (3); two stem cell retention plates (6) are fixedly connected to the upper and lower parts of the middle part of the inner wall of the extraction device body (1); the middle parts of the two stem cell retention plates (6) are fixedly connected to a stem cell extraction plate (7); a plasma retention plate (24) is installed on the bottom of the inner wall of the extraction device body (1); a material discharge assembly is installed inside the extraction device body (1); and a cleaning assembly is arranged on the right part of the extraction device body (1); The material discharge assembly comprises two sets of gear rings (8) and a stem cell push plate (9). The two sets of gear rings (8) are rotatably connected to the middle of the two stem cell retention plates (6). The stem cell push plate (9) is fixedly connected to one side of the inner wall of the gear ring (8). The bottom of the stem cell push plate (9) is attached to the top of the stem cell extraction plate (7). One side of the stem cell retention plate (6) is penetrated and provided with a stem cell discharge port (10). The stem cell discharge port (10) cooperates with the stem cell push plate (9). One side of the interior of the stem cell retention plate (6) is provided with a gear groove (11). The middle part of the interception plate (24) is rotatably connected to a second gear ring (25), one side of the inner wall of the second gear ring (25) is fixedly connected to a plasma push plate (26), one side of the plasma push plate (26) penetrates and is provided with a plasma discharge port (27), the bottom of the extraction device body (1) is fixedly connected to an output box (12), the bottom of the output box (12) is fixedly connected to an output box (13), a motor (14) is installed at the bottom of the output box (13), the output end of the motor (14) penetrates the output box (13) and is fixedly connected to a rotating shaft (15), the rotating shaft (15 ) is fixedly connected to a gear 1 (16) at the middle of the outer wall of the output box 2 (13), the gear 1 (16) rotates inside the output box 2 (13), an output slot (17) is provided on one side of the inner part of the output box 1 (12), a synchronous wheel 1 (18) is fixedly connected to the top of the outer wall of the rotating shaft (15), a rotating rod (19) passes through and is rotatably connected to one side of the output box 1 (12), the rotating rod (19) passes through the extraction device body (1), the plasma retention plate (24) and the stem cell retention plate (6) and is rotatably connected, the outer wall bottom of the rotating rod (19) is fixedly connected to a synchronous wheel 2 (20), the synchronous wheel The second synchronous wheel (20) is located in the output groove (17); the outer wall of the second synchronous wheel (20) is sleeved with a synchronous belt (21); the other end of the synchronous belt (21) is sleeved on the first synchronous wheel (18); the rotating rod (19) is located on the outer wall of the plasma retention plate (24) and is fixedly connected with a second gear (22); the second gear (22) is meshingly connected with a second gear ring (25); the side of the rotating rod (19) located inside the stem cell retention plate (6) is fixedly connected with two third gears (23); the third gear (23) rotates in the gear groove (11) and is meshingly connected with the first gear ring (8).

2. The cord blood hematopoietic stem cell extraction and separation device according to claim 1, characterized in that: The cleaning assembly comprises a water tank (30), a water pump (31) and a water pipe (32). The water tank (30) is arranged at the right part of the extraction device body (1). The water pump (31) is installed at the lower part of the water tank (30) close to the extraction device body (1). The water pipe (32) is fixedly connected to the output end of the water pump (31). The end of the water pipe (32) away from the water pump (31) passes through the output box (13). A water groove (33) is provided on one side of the interior of the output box (13). The water groove (33) cooperates with the output box (13). A sleeve (34) is rotatably connected to the top of the water groove (33). The sleeve (34) passes through the output box (13) and the output box (12) and is fixedly connected to the extraction device body (1). A guide block (35) is fixedly connected to one side of the inner wall of the sleeve (34). The inner side of the sleeve (34) is rotatably connected to the inner side of the sleeve (34). The second water pipe (36) is rotatably connected to the second water pipe (36). The second water pipe (36) is provided with a guide groove (37) on the inner outer wall of the sleeve (34). The guide groove (37) cooperates with the guide block (35). The bottom of the outer wall of the sleeve (34) is fixedly connected with a gear four (38). The gear four (38) is meshingly connected with the gear one (16). The outer wall of the second water pipe (36) passes through the stem cell retention plate (6) and is rotatably connected thereto. Three angled nozzles (39) are fixedly connected to the outer wall of the second water pipe (36). A right-angled nozzle (40) is fixedly connected to the side of the outer wall of the second water pipe (36) away from the angled nozzle (39). The angled nozzle (39) cooperates with the stem cell retention plate (6) and the plasma retention plate (24). The right-angled nozzle (40) cooperates with the inner wall of the extraction device body (1). A water inlet (44) is provided at the top of the water tank (30).

3. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 2, characterized in that: The top of the outer wall of the water pipe one (32) is penetrated and fixedly connected with the water pipe three (41), and the top of the water pipe three (41) is fixedly connected with the annular tube (42), the annular tube (42) is sleeved on the upper part of the outer wall of the extraction device body (1), and the inner wall of the annular tube (42) is fixedly connected with a nozzle (43), and the nozzle (43) penetrates the extraction device body (1) and cooperates with the crushing net (5).

4. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 3, characterized in that: A plasma collection box (28) is fixedly connected to one side of the outer wall of the extraction device body (1), and the plasma collection box (28) cooperates with the plasma discharge port (27).

5. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 4, characterized in that: Two stem cell collection boxes (29) are provided on one side of the outer wall of the extraction device body (1) and located above the plasma collection box (28). The stem cell collection boxes (29) are connected to the stem cell discharge port (10). The plasma collection box (28) and the stem cell collection box (29) are both provided with a material extraction port (48) on the side away from the extraction device body (1).

6. The cord blood hematopoietic stem cell extraction and separation device according to claim 5, characterized in that: A loading door (45) is provided at the upper portion of the side of the outer wall of the extraction device body (1) away from the water tank (30), a stem cell inspection door (46) is provided at the upper and lower portions of the middle portion of the side of the outer wall of the extraction device body (1) away from the water tank (30), and a plasma inspection door (47) is provided at the lower portion of the side of the outer wall of the extraction device body (1) away from the water tank (30).

7. The cord blood hematopoietic stem cell extraction and separation device according to claim 6, characterized in that: The two stem cell retention plates (6) are both installed obliquely in the extraction device body (1) to promote material flow during material discharge and cleaning.

8. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 7, characterized in that: The inner wall of the plasma retention plate (24) is arranged in an inclined state, so that the plasma and impurities inside can be cleaned more thoroughly during feeding and cleaning.

9. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 8, characterized in that: The stem cell retention plate (6) is divided into an upper and lower side. The stem cell extraction plate (7) inside the upper stem cell retention plate (6) can retain stem cells, and the stem cell extraction plate (7) inside the lower stem cell retention plate (6) can retain white blood cells.

10. The device for extracting and separating hematopoietic stem cells from umbilical cord blood according to claim 9, characterized in that: The four corners of the bottom of the extraction device body (1) are all fixedly connected with support feet (49).

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