Bactericidal composition for preventing and treating rot disease

Abstract

The invention relates to a bactericidal composition for preventing and treating rot disease, the bactericidal composition is compounded by oxine-copper and coumoxystrobin, the mass ratio of oxine-copper to coumoxystrobin is (50: 1)-(1: 20), the bactericidal composition shows a remarkable synergistic effect in a certain range, and the co-toxicity coefficient can reach 141.85. The bactericidal composition has a remarkable synergistic effect on fungal diseases such as apple rot and poplar rot, is superior to a single agent in quick-acting and persistent effects, is beneficial to reduction of the use amount and residue of pesticides, and is beneficial to environmental protection and treatment of agricultural diseases.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a bactericidal composition for preventing and treating rot diseases. Background Art

[0002] Copper quinolate, CAS No.: 10380-28-6, English name: Copper quinolate, Molecular formula: C 18 H 12 CuN 2 O 2 , relative molecular weight: 351.84.

[0003] Quinoline copper is a broad-spectrum, high-efficiency, low-residue organic chelated copper fungicide that has good preventive and therapeutic effects on fungal and bacterial diseases. It contains organic quinoline and copper salts with dual fungicidal activity, which is rooted in its unique mechanism of action. It can not only inhibit the G-protein synthesis of early cells of pathogens, that is, block physiological and biochemical reactions to inhibit the division and differentiation of pathogen cells, but also has multi-point contact activity. Chelated organic copper acts on the germinating spores of pathogens at multiple points, thereby achieving the effects of prevention and treatment.

[0004] At present, the registered crops and control targets of quinoline copper include tomato late blight, cucumber bacterial angular leaf spot, downy mildew, grape downy mildew, Dendrobium officinale soft rot, bayberry cancer and brown spot, litchi downy mildew, citrus canker, resin disease and ring rot, potato early blight, kiwi canker and blossom end rot, and pecan dry rot.

[0005] Syringoxystrobin, CAS No.: 850881-70-8, English name: coumoxystrobin, molecular formula: C 26 H 28 O 6 , relative molecular weight: 436.49.

[0006] Syringoxystrobin belongs to the methoxyacrylate class and is a protective fungicide that also has certain therapeutic effects. Its mechanism of action is to inhibit mitochondrial electron transfer by binding to the cytochrome bcl complex, thereby destroying the energy synthesis of the pathogen and playing a bactericidal role.

[0007] At present, the registered crops and control targets of thujastrobin include apple tree rot, cucumber downy mildew and citrus scab.

[0008] Among various diseases, fruit rot occurs in countries around the world, infecting a variety of deciduous fruit trees and garden tree species such as apple, pear, peach, plum, willow and poplar. Among them, fruit rot is serious, has a high recurrence rate, and is difficult to cure. It seriously affects fruit quality, yield and fruit life, and even causes garden destruction, restricting the development of forestry and fruit industry [Shanxi Agricultural Science 2022, 50(3):419-423].

[0009] Among all kinds of fruit tree rot diseases, apple rot is a type of branch and trunk disease with a wide range of occurrence and serious damage. Its pathogen is apple rot bacteria, and the sexual generation (Valsa ceratosperma) belongs to the Ascomycetes-Ascomycetes-Valsa ceratosperma, which is a weak parasitic fungus. The hyphae invade through leaf scars, fruit stalk scars, lenticels or dead tissue on the bark surface, and there is a possibility of lurking in them, often causing local branch rot to form scars, hindering nutrient transport, and in severe cases causing plant death.

[0010] Tree trunk rot is a disease caused by Cytospora fungi and their sexual forms, Valsa, which can cause large areas of forests and woody plants to die. It is widespread around the world, causing serious damage and is very difficult to prevent and control. Among them, the probability of poplar rot increases significantly with the increase in the planting area of ​​poplars, greatly restricting the healthy growth of forests. [Chinese Science and Technology Journal Database (Full Text Edition) Natural Science 2021 Issue 9 0056-0056, 58, 2 pages in total].

[0011] CN102283225A discloses a fungicide composition containing syringoxystrobin and protective fungicides mancozeb, mancozeb, metiram and propineb, and discloses that the fungicide composition is used for preventing and treating apple leaf spot disease, ring rot, anthracnose and brown spot disease, wherein the prevention and treatment target is fruit, but does not disclose protective measures for fruit tree branches and trunks.

[0012] CN113712031A discloses a fungicide composition of syringoxystrobin and berberine, quinoline copper or ningnanmycin, and discloses that the fungicide composition is used to control sugarcane brown spot disease, the control object is leaves, and the control target is sugarcane brown spot pathogen (Cercospora longipes), which belongs to the phylum Ascomycota, class Ascomycetes and genus Cercospora, but does not disclose protective measures for apple branches.

[0013] The present invention is significantly different from the present invention in that: 1) the control objects are different. The composition of the patent is used for sugarcane, which is a herbaceous plant, while the composition of the present invention is used for poplar, willow, elm, bayberry or apple trees, which are all woody plants; 2) the control targets are different. The sugarcane brown spot pathogen (Cercospora longipes) described in the patent belongs to the Ascomycetes-Ascomycetes-Cercospora, and mainly harms corn, beets, celery or mung beans, etc., and directly infects the leaves. The composition of the present invention, syringoxystrobin and quinoline copper, controls the apple rot pathogen, whose sexual generation (Valsa ceratosperma) belongs to the Ascomycetes-Ascomycetes-Valsa ceratosperma, and the asexual generation (Cytospora) belongs to the Ascomycetes-Cytospora, and the rot disease mainly harms poplar, willow, elm, bayberry or apple trees, and infects branches and trunks through natural openings or wounds. The fungi of the two diseases are completely different, and their life history and infection target methods on different control objects are different. The active ingredients have significant differences in their preventive effects on different crops and different fungal diseases. The disclosed patent is for the prevention and treatment of sugarcane brown spot disease, while the present invention is for the prevention and treatment of fruit tree rot diseases. There is no direct correlation between the two. So far, there has been no report on the synergistic antibacterial effect of quinoline copper and syringoxystrobin on rot diseases, especially apple rot and poplar rot. The inventors have found through several experiments that quinoline copper and syringoxystrobin have a synergistic antibacterial effect on rot diseases, especially apple rot and poplar rot. Summary of the invention

[0014] The purpose of the present invention is to provide a fungicide composition for preventing and controlling rot diseases, which has a significant synergistic prevention and control effect on apple rot diseases and poplar rot diseases, increases the scope of crop disease prevention and control, and provides an effective solution for agricultural disease prevention and control.

[0015] In order to achieve the above object, the present invention provides the following technical solutions:

[0016] A bactericidal composition for preventing and treating rot disease, the composition consists of quinoline copper and syringoxystrobin, wherein the mass ratio of quinoline copper to syringoxystrobin is 50:1-1:20, and the total mass of quinoline copper and syringoxystrobin accounts for 0.1%-90% of the total mass of the bactericidal composition.

[0017] Furthermore, the mass ratio of quinoline copper to syringoxystrobin is 30:1-1:10, and the total mass of quinoline copper and syringoxystrobin accounts for 1%-60% of the mass of the fungicide composition.

[0018] Furthermore, the mass ratio of quinoline copper to syringoxystrobin is 15:1, and the total mass of quinoline copper and syringoxystrobin accounts for 2%-50% of the total mass of the fungicide composition.

[0019] The fungicidal composition of the present invention can be prepared into various dosage forms, including water-dispersible granules, wettable powders, suspensions, dispersible oil suspensions, suspoemulsions, pastes, dispersible oil suspensions, suspoemulsions or suspension-microcapsule suspensions.

[0020] Furthermore, when the dosage form of the bactericidal composition is water-dispersible granules, the sum of the quinoline copper and syringoxystrobin is 6%-60% by weight, and the composition further includes 4%-14% of a dispersant, 1%-4% of a wetting agent, and 22%-89% of a filler.

[0021] Furthermore, the dosage form of the fungicide composition is a wettable powder, in which the sum of the copper quinoline and syringoxystrobin is 9%-62% by weight, and the composition further comprises 5%-10% of a dispersant, 1%-5% of a wetting agent, and 23%-85% of a filler.

[0022] Furthermore, the dosage form of the bactericidal composition is a suspension, in which the sum of quinoline copper and syringoxystrobin is 8%-40% by weight, and the composition also includes 3%-6% of a dispersant, 0.5%-2% of a wetting agent, 0.3%-1.2% of a thickener, 0.2% of a defoaming agent, 0.2% of a preservative, and 5% of an antifreeze agent.

[0023] Furthermore, the dosage form of the bactericidal composition is an ointment, in which the sum of the quinoline copper and the syringoxystrobin is 0.4%-10% by weight, and further comprises 0.5%-3% of a dispersant, 60%-85% of a thickener, 0.1% of a defoaming agent, and 5%-10% of an antifreeze agent.

[0024] The fungicidal composition of the present invention also contains common adjuvants required for preparing pesticide preparations, including one or a mixture of dispersants, wetting agents, solvents, emulsifiers, thickeners, stabilizers, pH regulators, antifreeze agents, defoamers, preservatives, disintegrants and fillers, etc. The above are all commonly used or permitted ingredients in pesticide preparations, without special restrictions, and the specific types and amounts are determined according to experiments.

[0025] Furthermore, the dispersant can be selected from anionic, nonionic, polymeric and inorganic types, including but not limited to sulfonates, polycarboxylates, phosphates, sulfates, polyoxyethylene polyoxypropylene ether block copolymers, alkylphenol polyoxyethylene phosphates, alkylbenzene sulfonate calcium salts, alkylphenol polyoxyethylene ethers, fatty acid polyoxyethylene esters, fatty amine polyoxyethylene ethers and glycerol fatty acid ester polyoxyethylene ethers. One or more.

[0026] Furthermore, the wetting agent includes, but is not limited to, one or more of alkyl sulfates, alkyl naphthalene sulfonates, alcohol ether sulfonates, alkylphenol polyethers, fatty alcohol polyethers, EO-PO block polyethers, alkylaryl polyoxyethylene ethers, alkylphenol polyoxyethylene ether formaldehyde condensates, alkylphenol polyoxyethylene ether phosphates, trisiloxane polyethers, phenethylphenol polyoxyethylene ether phosphates, sodium methylnaphthalene sulfonate formaldehyde condensates, tea dregs, saponin powder, silkworm feces, soapberry powder and laundry detergent.

[0027] Further, the solvent includes but is not limited to C 9 and C 10 One or more of mixed aromatic hydrocarbons, xylene, toluene, cyclohexanone, N,N-dimethylformamide, dimethyl sulfoxide, methanol, ethanol, propanol, butanol, isopropanol, N-octyl pyrrolidone, N-methyl pyrrolidone, ethyl acetate and vegetable oil.

[0028] Further, the emulsifier type can be selected from non-ionic and anionic, including but not limited to Nongru 600# series (phenylethylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), Nongru 700# (alkylphenol formaldehyde resin polyoxyethylene ether), ethylene oxide-propylene oxide block copolymer, styrene polyoxyethylene ether ammonium sulfate, Nongru 400# (styrene formaldehyde resin polyoxyethylene ether), NP series (nonylphenol polyoxyethylene ether), Tween series (sorbitan fatty acid ester polyoxyethylene ether), AEO series (fatty alcohol polyoxyethylene ether), Span series (sorbitan monostearate) and 500# (calcium dodecylbenzene sulfonate) One or more.

[0029] Furthermore, the thickener includes, but is not limited to, one or more of xanthan gum, magnesium aluminum silicate, bentonite, hydroxymethyl (ethyl) cellulose, methyl cellulose, polyvinyl alcohol, polyacrylate and polyurethane.

[0030] Furthermore, the antifreeze agent includes, but is not limited to, one or more of ethylene glycol, propylene glycol, glycerol, urea and inorganic salts.

[0031] Furthermore, the defoaming agent includes, but is not limited to, one or more of an organosilicon defoaming agent, a 20% polydimethylsiloxane emulsion, and polyurea.

[0032] Furthermore, the preservative includes, but is not limited to, one or more of benzisothiazolinone, sodium benzoate, benzoic acid, formaldehyde, butyl paraben, dimethyl fumarate and potassium sorbate.

[0033] Furthermore, disintegrants include, but are not limited to, one or more of ammonium sulfate, urea, bentonite, citric acid, succinic acid and sodium bicarbonate.

[0034] Furthermore, the filler includes, but is not limited to, one or more of corn starch, ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, white carbon black, kaolin, light calcium carbonate, talc, attapulgite, clay, pumice, broken brick, blister stone or bentonite and non-adsorbent calcareous soil or sand.

[0035] On the other hand, the fungicidal composition of the present invention can be used to prevent and control fungal diseases of crops. Furthermore, the fungicidal composition of the present invention can be used to prevent and control fungal diseases of fruit trees and garden and horticultural crops, wherein fruit trees include apples, pears, peaches and plums, and garden and horticultural crops include willows and poplars.

[0036] Furthermore, the diseases include apple rot, poplar rot and willow rot, etc., and the pathogens thereof belong to the genus Ascomycota of the phylum Ascomycota.

[0037] The fungicidal composition of the present invention can act on pathogenic bacteria or their environment, or on plants, plant parts, plant propagation materials and subsequently grown plant organs, soil or cultivation media, materials or spaces.

[0038] On the other hand, the present invention also includes a method for using the fungicidal composition, which can be applied in an agronomically effective and substantially non-toxic application amount by seed treatment, stem application, soaking, dripping, pouring, spraying, spraying, dusting, scattering or fumigation.

[0039] Compared with the prior art, the present invention has the following advantages:

[0040] 1. The inventors have found through several experiments that the combination of quinoline copper and syringoxystrobin shows significant synergy within a certain range, and the co-toxicity coefficient can reach 141.85, which has obvious synergistic effect, can improve the control effect of apple rot and poplar rot, reduce the amount of pesticide application, reduce residues, and alleviate environmental pollution.

[0041] 2. The inventors found that, due to the different mechanisms of action of the active ingredients, the combination of quinoline copper and syringoxystrobin can delay the development of drug resistance, reduce the frequency of application, and obtain better prevention and control effects, which is in line with the "reduced dosage and increased efficiency" medication policy. DETAILED DESCRIPTION

[0042] In order to better understand the essence of the present invention, the contents of the present invention are further illustrated in conjunction with the embodiments below, but they cannot be regarded as limiting the present invention. The following description is only used to explain the present invention. Any modifications, substitutions or improvements made without departing from the spirit and principles of the present invention are all within the scope of protection required by the present invention.

[0043] Indoor activity measurement method

[0044] The inventors conducted a large number of target tests and took the virulence test of apple rot bacteria (Valsa mali) as an example to conduct indoor activity determination demonstration.

[0045] The test was conducted with reference to the Agricultural Industry Standard of the People's Republic of China NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth", and the test standards are as follows:

[0046] 1. Experimental purpose: To determine the toxicity of different compound combinations to apple rot pathogens in the laboratory;

[0047] 2. Experimental conditions

[0048] 2.1. Test target: apple rot pathogen (Valsa mali);

[0049] 2.2. Culture conditions: constant temperature incubator, temperature 28°C, photoperiod L:D=16:8h;

[0050] 2.3. Experimental instruments: electronic balance, pipette, culture dish, hole puncher, inoculator, constant temperature incubator.

[0051] 3. Test methods

[0052] 3.1. Prepare activated apple rot bacteria (Valsa mali), select an appropriate amount of bacteria, and culture them until they grow well and consistently.

[0053] 3.2. Dissolve the original drug with deionized water or an organic solvent, and then dilute it with an aqueous solution containing an appropriate amount of a surfactant (such as 0.1% Tween 80 or 0.1% Triton-X100). Prepare a single-dose stock solution; and set the dilution concentration gradient of the test drug according to the purpose of the mixing, the activity of the drug, etc., and conduct preliminary tests on mixtures with different ratios. On this basis, determine the test concentration range of each mixture. Set 5 concentration treatments for each drug according to the content of the active ingredient, set 3 replicates, and set a solvent solution without the drug as a control;

[0054] 3.3. According to the experimental design, take the drug solution from low concentration to high concentration in sequence, add it to the pre-melted PDA culture medium under sterile conditions, shake it thoroughly, and make a plate treated with drug solution; set 3 replicates for each group of treatment, and make the final concentration of each group of treatment the required concentration respectively;

[0055] 3.4. Take the pre-cultured pathogen plates and, under sterile conditions, cut bacterial cakes from the edge of the colony using a 5 mm diameter borer. Use an inoculator to inoculate the center of each plate treated with the drug solution. Place the plates in a constant temperature incubator at 28°C and a light cycle of L:D = 16:8h for 5-7 days.

[0056] 4. Experimental investigation and calculation method:

[0057] The colony diameter was measured before and after treatment, and the colony diameter was measured vertically once using a vernier caliper using the cross method, and the average value was taken. The colony diameters of the control group and each treatment group were recorded, and the colony growth inhibition rate was calculated. On this basis, the toxicity curve was fitted and the EC was calculated. 50 .

[0058] Colony diameter change = colony diameter after treatment - colony diameter before treatment

[0059]

[0060] The combined effect is calculated with reference to the Agricultural Industry Standard of the People's Republic of China NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6: Determination of Combined Effects of Mixtures". The calculation method is as follows:

[0061]

[0062] Theoretical Toxicity Index (TTI)

[0063] =A measured toxicity index × A percentage in the mixture

[0064] + Actual toxicity index of agent B × percentage of agent B in the mixture

[0065]

[0066] The co-toxicity coefficient (CTC) of the mixture was calculated according to the Sun Yunpei method to evaluate the type of combined action. CTC ≥ 120 showed synergistic effect, CTC ≤ 80 showed antagonistic effect, and CTC between 80 and 120 showed additive effect.

[0067] Indoor Activity Assay Example

[0068] According to a large number of mixed test verifications, the inventors used apple rot bacteria as the test target, took quinoline copper and thujamycin at a ratio of 45:1, 30:1, 15:1, 1:1, and 1:15, a total of 5 ratios, to conduct indoor toxicity test and the results are shown below. The experimental design is shown in Table 1:

[0069] Table 1 Experimental design for toxicity determination of copper quinoline and syringoxystrobin

[0070]

[0071] The doses shown in Table 1 were used for testing, and the colony diameters of each treatment were counted. The colony growth inhibition rate of each treatment group was obtained by data analysis, and the toxicity baseline and EC of quinoline copper and syringoxystrobin were calculated. 50The values ​​were calculated and the combined effects were evaluated. The results are shown in Table 2:

[0072] Table 2 Evaluation of the combined effects of five compound ratios of quinoline copper and thujamycin on apple rot pathogens

[0073]

[0074] As shown in Table 2, the co-toxicity coefficients of the five ratios of quinoline copper and syringoxystrobin ranged from 101.23 to 141.85, and different ratios had different joint action modes on apple rot pathogens. Among them, the co-toxicity coefficient value of quinoline copper:syringoxystrobin ratio of 30:1 was 127.47, showing a synergistic effect; further, the co-toxicity coefficient value of quinoline copper:syringoxystrobin ratio of 15:1 was 141.85, showing a significant synergistic effect.

[0075] The above mixing test of quinoline copper and thujastrobin shows that the two are synergistic. Their combined use can improve the control effect, reduce the dosage, save resources and reduce the agricultural cost of the product.

[0076] According to the results of the toxicity test, the inventors prepared compound preparations and selected some of them for presentation of the results.

[0077] Formulation Example 1: 30% quinoline copper + 2% syringoxystrobin water dispersible granules

[0078] The preparation method comprises the following steps: 30% quinoline copper, 2% syringoxystrobin, 2% sodium salt of polycarboxylate, 6% alkylnaphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 40% corn starch and 100% diatomaceous earth. The above materials are mixed, air flow crushed, mixed, granulated, dried, sieved and tested to obtain a product that meets the requirements.

[0079] Formulation Example 2: 30% quinoline copper + 1% syringoxystrobin water dispersible granules

[0080] The preparation method comprises the following steps: 30% quinoline copper, 1% syringoxystrobin, 2% sodium salt of polycarboxylate, 6% alkylnaphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 40% corn starch, and 100% ammonium sulfate. The above materials are mixed, air flow crushed, mixed, granulated, dried, sieved, and tested to be qualified to obtain the product.

[0081] Formulation Example 3: 3% quinoline copper + 3% syringoxystrobin water dispersible granules

[0082] The preparation method comprises the following steps: 3% quinoline copper, 3% syringoxystrobin, 4% alkylnaphthalene sulfonate formaldehyde condensate, 1% alkylnaphthalene sulfonate, 30% kaolin, and 100% diatomaceous earth. The above materials are mixed, air flow crushed, mixed, granulated, dried, sieved, and tested to be qualified to obtain the product.

[0083] Formulation Example 4: 15% quinoline copper + 45% syringoxystrobin water dispersible granules

[0084] The preparation method comprises 15% quinoline copper, 45% syringoxystrobin, 6% sodium lignin sulfonate, 8% alkylnaphthalene sulfonate formaldehyde condensate, 2% alkylnaphthalene sulfonate, 2% sodium dodecyl sulfate and 100% diatomaceous earth. The above materials are mixed, air flow crushed, mixed, granulated, dried, sieved and tested to obtain a product as qualified product.

[0085] Formulation Example 5: 3% quinoline copper + 6% syringoxystrobin wettable powder

[0086] Quinoline copper 3%, syringoxystrobin 6%, alkylnaphthalene sulfonate formaldehyde condensate 5%, pulverized powder 1%, white carbon black 10%, ammonium sulfate 30%, kaolin (to make up 100%), the above materials are mixed, air flow crushed, mixed and tested to obtain the product.

[0087] Formulation Example 6: 10% quinoline copper + 10% syringoxystrobin wettable powder

[0088] The preparation method is prepared by mixing 10% quinoline copper, 10% syringoxystrobin, 10% alkylnaphthalene sulfonate formaldehyde condensate, 3% pulverized powder, 5% white carbon black, 30% ammonium sulfate and 100% kaolin. The above materials are pulverized by air flow, mixed and tested to be qualified.

[0089] Formulation Example 7: 45% quinoline copper + 3% syringoxystrobin wettable powder

[0090] The preparation method is prepared by mixing 45% quinoline copper, 3% syringoxystrobin, 10% sodium lignin sulfonate, 3% sodium dodecyl sulfate, 5% white carbon black and 100% kaolin. The above materials are pulverized by air flow, mixed and tested to be qualified.

[0091] Formulation Example 8: 60% quinoline copper + 2% syringoxystrobin wettable powder

[0092] Quinoline copper 60%, syringoxystrobin 2%, sodium lignin sulfonate 5%, alkyl naphthalene sulfonate formaldehyde condensate 4%, sodium polycarboxylate 1%, sodium isopropyl naphthalene sulfonate 5%, white carbon black 3%, diatomaceous earth to make up 100%, the above materials are mixed, air flow crushed, mixed, and tested to obtain a qualified suspension. Formulation Example 9: 7.5% quinoline copper + 0.5% syringoxystrobin suspension

[0093] Quinoline copper 7.5%, syringoxystrobin 0.5%, alkylnaphthalene sulfonate formaldehyde condensate 3%, alkylnaphthalene sulfonate 0.5%, ethylene glycol 5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, organic silicon defoamer 0.2%, benzisothiazolinone (20% BIT) 0.2%, water to 100%. The above materials are mixed, ground and high-speed sheared, and tested to be qualified to obtain the product.

[0094] Preparation Example 10: 9% quinoline copper + 0.3% syringoxystrobin suspension

[0095] Quinoline copper 9%, syringoxystrobin 0.3%, alkylnaphthalene sulfonate formaldehyde condensate 3%, alkylnaphthalene sulfonate 0.5%, propylene glycol 5%, xanthan gum 0.3%, organosilicon defoamer 0.2%, benzisothiazolinone (20% BIT) 0.2%, water to 100%. The above materials are mixed, ground and high-speed sheared, and tested to be qualified to obtain the product.

[0096] Preparation Example 11: 12% quinoline copper + 12% syringoxystrobin suspension

[0097] Quinoline copper 12%, syringoxystrobin 12%, alkylnaphthalene sulfonate formaldehyde condensate 4%, alkylnaphthalene sulfonate 1%, glycerol 5%, xanthan gum 0.2%, magnesium aluminum silicate 0.5%, organic silicon defoamer 0.2%, benzisothiazolinone (20% BIT) 0.2%, water to 100%. The above materials are mixed, ground and high-speed sheared, and tested to be qualified to obtain the product.

[0098] Preparation Example 12: 15% quinoline copper + 25% syringoxystrobin suspension

[0099] Quinoline copper 15%, syringoxystrobin 25%, phosphate amine salt 3%, polycarboxylic acid sodium salt 3%, ethylene oxide-propylene oxide block copolymer 2%, ethylene glycol 5%, xanthan gum 0.15%, magnesium aluminum silicate 0.5%, organic silicon defoamer 0.2%, sodium benzoate 0.5%, water to 100%. The above materials are mixed, ground and high-speed sheared, and tested to be qualified to obtain the product.

[0100] Preparation Example 13: 1.5% quinoline copper + 0.1% syringoxystrobin ointment

[0101] 1.5% quinoline copper, 0.1% syringoxystrobin, 0.5% alkylnaphthalene sulfonate formaldehyde condensate, 70% polyacrylate, 10% ethylene glycol, 0.2% xanthan gum, 1.5% magnesium aluminum silicate, 0.1% organic silicon defoamer, and water to 100%. The above materials are mixed, ground, and remixed, and tested to be qualified to obtain the product.

[0102] Preparation Example 14: 3% quinoline copper + 0.1% syringoxystrobin ointment

[0103] 3% quinoline copper, 0.1% syringoxystrobin, 0.5% alkylnaphthalene sulfonate formaldehyde condensate, 70% polyacrylate, 5% ethylene glycol, 0.2% xanthan gum, 1.5% magnesium aluminum silicate, 0.1% organic silicon defoamer, and water to 100%. The above materials are mixed, ground, and remixed, and tested to be qualified to obtain the product.

[0104] Preparation Example 15: 0.2% quinoline copper + 0.2% syringoxystrobin ointment

[0105] 0.2% quinoline copper, 0.2% syringoxystrobin, 0.4% phosphate amine salt, 85% polyacrylate, 5% propylene glycol, 0.3% xanthan gum, 0.1% organosilicon defoamer, and 100% water. The above materials are mixed, ground, and remixed, and tested to be qualified.

[0106] Preparation Example 16: 3% quinoline copper + 7% syringoxystrobin ointment

[0107] 3% quinoline copper, 7% syringoxystrobin, 1% phosphate amine salt, 2% alkylnaphthalene sulfonate formaldehyde condensate, 60% polyurethane, 5% glycerol, 0.2% xanthan gum, 0.5% magnesium aluminum silicate, 0.2% organic silicon defoamer, and 100% water. The above materials are mixed, ground, and remixed, and tested to be qualified.

[0108] Product Field Efficacy Method

[0109] According to the product formulation examples, the inventors used apple rot bacteria as the test object and conducted field efficacy tests on the above formulations.

[0110] Spray method

[0111] The test was conducted with reference to the Agricultural Industry Standard of the People's Republic of China GB / T 17980.117-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 117: Fungicides for Control of Apple and Pear Rot Diseases". The test standards are as follows:

[0112] 1. Experimental purpose: To investigate the field control effect of different compound combinations on apple rot

[0113] 2. Target: Apple rot pathogen (Valsa mali)

[0114] 3. Test methods

[0115] 3.1. Application method: normal amount of foliar spray, 3 times in total, with an interval of 20 days between each application.

[0116] 3.2. Plot setting: The plots are arranged in random blocks, which are determined on site. There are about 10-15 plants in a plot, which is repeated 3 times.

[0117] 4. Experimental investigation and calculation method:

[0118] 4.1. Survey time: Surveys were conducted before spraying and 10 days after the third spraying, for a total of 2 surveys.

[0119] 4.2. Survey method: In each plot, 5 trees were randomly surveyed using the "five-point sampling method" to examine the disease status of the trees, and the total number of disease spots before drug treatment and the number of recurrent disease spots after drug treatment were recorded. When sampling, avoid the edge of the plot.

[0120] 4.3 Calculation method:

[0121]

[0122]

[0123] Scraping and smearing method

[0124] The test was conducted with reference to the Agricultural Industry Standard of the People's Republic of China GB / T 17980.117-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 117: Fungicides for Control of Apple and Pear Rot Diseases", and the field test method for apple rot disease control applied by Tian Xiaoli and Liu Shuanrong was adopted [Research on Control Agents for Apple Rot Disease. Agriculture and Technology, 2017, 37(4): 208-209.] The test description is as follows:

[0125] 1. Experimental purpose: To investigate the field control effect of different compound combinations on apple rot

[0126] 2. Target: Apple rot pathogen (Valsa mali)

[0127] 3. Test methods

[0128] 3.1. Application method: Before applying the pesticide, scrape off the marked lesions and their rotten tissues until the phloem is exposed, and apply the diluted pesticide evenly on the lesions until they are completely covered.

[0129] 3.2. Plot setting: The plots are arranged in random blocks, which are determined on site. There are about 10 plants in a plot, which is repeated 3 times.

[0130] 4. Experimental investigation and calculation method:

[0131] 4.1. Survey time: Surveys were conducted twice, before application and 3 months after application.

[0132] 4.2. Investigation method: Before applying the pesticide, select the diseased spots on the trees in the test plot, number them and mark the outer contours, and measure the length and width of the diseased spots. After applying the pesticide, investigate the recurrence of the marked diseased spots and measure the length and width of the diseased spots.

[0133] 4.3 Calculation method:

[0134]

[0135]

[0136] Estimated lesion area (mm 2 ) = lesion length × width

[0137]

[0138] The test shown below was conducted in Xianyang City, Shaanxi Province from April 20, 2021 to January 20, 2022. Four apple plantations were selected as the field efficacy test sites of the present invention. The planting area of ​​each test site is about 200-300 acres, and the planting varieties include Ruixue, Qincui, Qinguan, etc., open-air cultivation, normal irrigation and drainage conditions, general management level, and continuous low-level occurrence of apple rot. The cultivation specifications in the plantation are about 6.0m×4.0m / plant. The cultivation conditions (apple variety, soil type, water and fertilizer management, transplanting period, planting density, growth period, water and fertilizer management) in several treatment areas of each test site are relatively consistent. The results of the three field control tests of the above formulation examples on apple rot are as follows:

[0139] Product Field Efficacy Example 1

[0140] According to the above experimental investigation and calculation method, the inventor designed a field test program for spraying pesticides to control apple rot, calculated the recurrence rate of lesions and analyzed the control effect, as shown in Table 3:

[0141] Table 3 Design and results of field efficacy test on apple rot

[0142]

[0143] As shown in Table 3, the field control effect of the mixed products of quinoline copper and thujamycin in different ratios on apple rot is better than that of quinoline copper and thujamycin alone. The use amount of the product preparation application example 1 is diluted 4571 times, and the use amount of the active ingredient is 70 mg / L, and the control effect is the best, reaching 73.59%.

[0144] Product Field Efficacy Example 2

[0145] According to the above test investigation and calculation method, the inventor designed a field test program for spraying pesticides to control apple rot, calculated the recurrence rate of lesions and analyzed the control effect, as shown in Table 4:

[0146] Table 4 Design and results of field efficacy test on apple rot

[0147]

[0148] As shown in Table 4, the field control effect of the mixed products of quinoline copper and thujamycin in different ratios on apple rot is better than that of quinoline copper and thujamycin alone. The use amount of the product preparation application example 7 is diluted 4800 times, and the use amount of the active ingredient is 100 mg / L, and the control effect is the best, reaching 82.88%.

[0149] Product Field Efficacy Example 3

[0150] According to the above test investigation and calculation method, the inventor designed a field test program for spraying pesticides to control apple rot, calculated the recurrence rate of lesions and analyzed the control effect, as shown in Table 5:

[0151] Table 5 Design and results of field efficacy test on apple rot

[0152]

[0153] As shown in Table 5, the field control effect of the mixed products of quinoline copper and syringoxystrobin in different ratios on apple rot is better than that of quinoline copper and syringoxystrobin alone. The use amount of the product formulation application example 9 is diluted 1143 times, and the effective ingredient usage is 70 mg / L, with the best control effect, reaching 78.61%.

[0154] Product Field Efficacy Example 4

[0155] According to the above test investigation and calculation method, the inventor designed a field test program for the control of apple rot by scraping and smearing, and statistically analyzed the recurrence rate of lesions and the reduction rate of lesion area and the control effect, as shown in Table 6:

[0156] Table 6 Design and results of field efficacy test on apple rot

[0157]

[0158] As shown in Table 6, the field control effect of the mixed products of quinoline copper and thujamycin in different ratios on apple rot is better than that of quinoline copper and thujamycin alone. When the product preparation Example 13 is diluted 3.2 times, the amount of active ingredient used is 5000 mg / L, and the effect of scraping and applying is the best, with a control effect of 91.00%, and a lesion area reduction rate of 16.67%.

[0159] In summary, the fungicidal composition prepared by compounding quinoline copper and syringoxystrobin in the present invention not only has obvious synergistic effect compared with the existing preparations, but also has good prevention and control effect, slows down the generation of drug resistance, and is worthy of promotion in production and application.

[0160] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bactericidal composition for preventing and treating rot, characterized in that: The composition consists of quinoline copper and syringostyrin, wherein the mass ratio of quinoline copper to syringostyrin is 50:1-1:20, and the total mass of quinoline copper and syringostyrin accounts for 0.1%-90% of the mass of the sterilization composition.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of quinoline copper to syringostyrin is 30:1-1:10, and the total mass of quinoline copper and syringostyrin accounts for 1%-60% of the mass of the sterilization composition.

3. The bactericidal composition according to claim 1, characterized in that The composition can be prepared into various dosage forms, including water-dispersible granules, wettable powders, suspensions, pastes, dispersible oil suspensions, suspoemulsions or suspension-microcapsule suspensions.

4. The bactericidal composition according to claim 3, characterized in that When the composition is prepared as water-dispersible granules, the total mass of quinoline copper and syringoxystrobin is 6%-60%; when the composition is prepared as wettable powder, the total mass of quinoline copper and syringoxystrobin is 9%-62%; when the composition is prepared as a suspension, the total mass of quinoline copper and syringoxystrobin is 8%-40%; when the composition is prepared as an ointment, the total mass of quinoline copper and syringoxystrobin is 0.4%-10%.

5. The bactericidal composition according to claim 3, characterized in that The bactericidal composition also contains common adjuvants required for preparing pesticide preparations, and the common adjuvants are one or a combination of dispersants, wetting agents, solvents, emulsifiers, stabilizers, thickeners, pH regulators, defoamers, antifreeze agents and carriers.

6. The bactericidal composition according to claim 1, characterized in that The composition can be used for preventing and controlling fungal diseases of crops, and further, can be used for preventing and controlling fungal diseases of fruit trees and garden and horticultural crops.

7. The bactericidal composition according to claim 6, characterized in that The composition can be used for preventing and controlling apple rot and poplar rot.

8. The bactericidal composition according to claim 6, characterized in that The method of use is to apply the fungicide composition in an agronomically effective and substantially non-toxic application amount to plants, plant propagation materials and subsequently grown plant organs, cultivation media, materials or spaces by seed treatment, stem application, soaking, dripping, pouring, spraying, atomizing, dusting, spreading or fumigation.

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