Whitening and spot-fading mesoderm therapy composition and preparation method thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a whitening and spot-fading mesoderm therapy composition and a preparation method thereof. The composition provided by the invention is prepared from the following components in percentage by mass: 0.5 to 5 percent of glutathione, 0.5 to 4 percent of carnosine, 0.01 to 0.2 percent of oligopeptide-1, 0.1 to 0.5 percent of end-deficient collagen, 0.01 to 0.5 percent of nonapeptide-1, 0.5 to 5 percent of tranexamic acid, 0.1 to 5 percent of L-VC, 0.1 to 2 percent of vitamin b12, 0.1 to 2 percent of adenosine, 0.01 to 0.2 percent of DNA sodium, 0.1 to 1 percent of sodium hyaluronate, 0.01 to 0.5 percent of sodium hydrogen sulfite and 74.1 to 97.96 percent of sterilized normal saline. The composition disclosed by the invention is used in mesoderm therapy for whitening and fading spots, components are matched from four dimensions of melanin, sugar resistance, oxidation resistance and substrate repair, the spots are comprehensively and efficiently improved, and meanwhile, blackening can be prevented.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and particularly relates to a mesotherapy composition for whitening and lightening spots and a preparation method thereof. Background Art

[0002] Melanin in the skin exists in melanocytes in the basal layer of the epidermis. It is transferred to the stratum corneum cells through the dendrites of melanocytes, and then ascends to the stratum corneum with the epidermal cells. It is shed with the aging keratinocytes and excreted from the body. The external environment has a great influence on melanin. For example, under a large amount of ultraviolet radiation, it will cause excessive synthesis of melanin, which will accumulate in the stratum corneum and form pigmentary skin diseases. At the same time, with the increase of age, the metabolism of melanin is slow, which will also form pigmentary skin diseases. In addition, human skin tissue contains a large amount of collagen and elastin. These two proteins will undergo non-enzymatic saccharification reactions with free reducing sugars in the skin to form brown glycosylation products, which will make the skin lose elasticity and become dull and lusterless.

[0003] At present, the common methods of reducing skin pigmentation mainly include using topical and oral whitening and freckle removal products, laser / intense pulsed light to improve skin color, acid peeling, etc.; because of the skin barrier, the efficacy of topical products cannot reach the dermis, and the efficacy is weak. Moreover, most of these products are aimed at melanin, which makes it difficult to improve skin color problems comprehensively; fruit acid peeling / freckle removal usually uses high-concentration fruit acid to peel the epidermis to achieve the purpose of skin peeling, but the process is painful, and some people will also experience peeling, scabs, etc., and sensitive skin intolerance, and if sun protection is not in place after treatment, serious pigmentation problems will occur; laser / intense pulsed light freckle removal, that is, the laser / intense pulsed light emitted by the instrument is concentrated and penetrated through the skin to irradiate the pigment in the spot area. The pigment spots will be broken and metabolized under the strong laser irradiation, thereby reducing the spots. Improper operation of laser or intense pulsed light freckle removal can cause skin burns, scars, pigment loss and blisters. If the later repair and sun protection are not proper, it is easy to cause more serious pigmentation.

[0004] Taking into account aspects such as drug metabolism and drug toxicity and side effects, oral products also have various disadvantages.

[0005] In the medical field, how to correctly deliver drugs to the area where they need to be treated so that the efficacy can be maximized has always been a problem that scientists have been studying. The beauty industry is also facing the same problem. Scientific research has found that each layer of the skin has a unique structure. The mesoderm is located between the epidermis and the dermis, and is the key to the skin's absorption of the active ingredients of topical beauty products. The epidermis is the barrier of the human skin. Due to the molecular structure of topical beauty cosmetics, it is difficult to penetrate the epidermis and penetrate deep into the skin, so the effect is not significant. Mesotherapy is a way to deliver drugs to the mesoderm through a specific device. It has a fast and lasting effect, a short recovery period, and almost no side effects.

[0006] At present, there are still some shortcomings in the mesodermal method of improving skin color / freckles. For example, the mechanism is mostly related to the single aspect of melanin, and less consideration is given to factors such as basement membrane repair, saccharification reaction, and anti-oxidation. In addition, improper repair after operation will cause the skin to turn black again. In addition, the operation process requires strict instrument disinfection, otherwise it is easy to cause infection, and the operation is relatively cumbersome. Therefore, it is of great significance to develop a mesodermal composition that can effectively improve skin color / freckles and is simple to operate. Summary of the invention

[0007] In view of the above problems, the present invention provides a mesotherapy composition for whitening and lightening spots and a preparation method thereof. The composition provided by the present invention combines ingredients from four dimensions: melanin, anti-sugar, anti-oxidation, and base repair. After human efficacy tests, it can ultimately improve skin color problems such as spots in an all-round and efficient manner, and prevent the problem of dark spots returning.

[0008] In order to achieve the above technical effects, the technical solution adopted by the present invention is:

[0009] A mesotherapy composition for whitening and lightening spots consists of the following components and their mass percentages: 0.5-5% glutathione, 0.5-4% carnosine, 0.01-0.2% oligopeptide-1, 0.1-0.5% atelocollagen, 0.01-0.5% nonapeptide-1, 0.5-5% tranexamic acid, 0.1-5% L-VC, 0.1-2% vitamin B12, 0.1-2% adenosine, 0.01-0.2% sodium DNA, 0.1-1% sodium hyaluronate, 0.01-0.5% sodium bisulfite and 74.1-97.96% sterilized physiological saline.

[0010] Preferably, it is composed of the following components and their mass percentages: 3% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% atelocollagen, 0.3% nonapeptide-1, 3% tranexamic acid, 3% L-VC, 1% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate, 0.3% sodium bisulfite and 85.4% sterile saline.

[0011] Preferably, the atelocollagen is obtained by first hydrolyzing the collagen with protease and then removing the terminal peptide; it has a complete quaternary structure, and the production process mainly includes material pretreatment, wall breaking and crushing, solution extraction, separation and purification, etc.

[0012] Preferably, the sodium DNA, i.e., polydeoxyribonucleotide, is extracted by the following process: sodium DNA is extracted from salmon sperm DNA, and then derived by controlled purification and sterilization.

[0013] Preferably, the molecular weight of the sodium hyaluronate is selected from 500-800 KDa and 1000-1800 KDa.

[0014] The present invention also provides a method for preparing the composition, and the specific preparation process is as follows:

[0015] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0016] S2. Add sodium hyaluronate to 2 / 3 of sterile physiological saline, heat to 80-90° C., keep warm for 30-40 minutes, and stir continuously until the above components are completely dissolved and transparent, to obtain mixture I;

[0017] S3. Add sodium DNA to 1 / 3 sterile saline and heat to 40-50°C for 10-20 minutes with constant stirring until the above components are completely dissolved and transparent to obtain mixture II for later use;

[0018] S4, lowering the temperature of the mixture I obtained in step S2 to 40-50° C., then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and starting to cool and stir until uniformly stirred, then adding the mixture II obtained in step S3, and mixing thoroughly to obtain a composition I;

[0019] S5. Add glutathione to the composition I obtained in step S3, and mix well to obtain.

[0020] Preferably, the continuous stirring in steps S2 and S3 is slow stirring at a rotation speed of 50 to 100 rpm for 10 to 20 minutes.

[0021] Preferably, the cooling and stirring in step S4 is to gradually cool the mixture to 25° C. and stir the mixture at a speed of 50 to 100 rpm for 20 to 30 minutes.

[0022] The specific effects of each component involved in the present invention are as follows:

[0023] Sterile saline is used as the solvent; tranexamic acid and nonapeptide-1 mainly inhibit the synthesis of melanin and the transfer of melanin to the stratum corneum; glutathione and L-VC act to scavenge free radicals, prevent excessive melanin production, protect cells from free radical damage, and reduce pigments; the main functions of vitamin B12 and adenosine are to provide nutrients and promote metabolism; oligopeptide-1 is an ECM protein, an amino acid sequence in type I collagen, which can be released by proteases at the wound site. The released molecules can activate and regulate the skin repair process; atelocollagen is a collagen treated with proteolytic enzymes, with the terminal peptides removed and low immunogenicity. High-purity atelocollagen has many advantages in biocompatibility and optimizing collagen cell interactions, while reducing side effects; the main function of carnosine is to protect collagen, inhibit the occurrence of glycation reactions, and prevent the formation of brown glycosylation products.

[0024] DNA sodium, or polydeoxyribonudeotide, is extracted from salmon male testes. Its DNA fragments are similar to the human body structure, and the DNA base composition of salmon is 98% similar to that of human DNA. It has the function of promoting tissue regeneration and effectively relieving inflammation, and has significant effects in the regeneration and repair of human skin, ligaments and tendons; sodium bisulfite is an auxiliary material, and its main function is to ensure that the composition is stable and not oxidized during the validity period. The main function of sodium hyaluronate is to provide moisture to the skin, accelerate skin repair, and make the skin supple and shiny.

[0025] In the process of studying the whitening and spot-lightening effect, the present invention has always avoided the side effects of chemical substances on the skin. Finally, glutathione, carnosine, tranexamic acid, L-VC and other ingredients are mainly used, supplemented by oligopeptide-1, atelocollagen, nonapeptide-1, vitamin B12, adenosine, DNA sodium, sodium hyaluronate and a trace of sodium bisulfite, which can effectively inhibit the formation of melanin. In the actual application process, it should be noted that glutathione needs to be added to the composition when the mesodermal treatment introduction operation is performed, and it is ready for use within 30 minutes. This is because there is an active sulfhydryl group (-SH) in the glutathione structure, which is the structural basis of its physiological function. However, the sulfhydryl group can make glutathione smelly and unstable, so it needs to be used within 30 minutes to maintain the integrity of the glutathione structure.

[0026] Currently, most mesotherapy solutions for improving skin color and pigmentation are single-pathway, with less consideration of factors such as base repair, anti-glycation, and antioxidant. The present invention not only involves the mechanism of melanin formation, but also the effects of glycation, oxidation, and repair mechanisms on skin color. By combining ingredients from four dimensions: melanin, anti-glycation, antioxidant, and base repair, it can comprehensively and efficiently improve skin color problems such as pigmentation. At the same time, the freeze-dried powder system also has corresponding anti-inflammatory repair ingredients, which can repair inflammation in a timely manner and prevent the occurrence of problems such as darkening.

[0027] Compared with the prior art, the present invention has the following advantages:

[0028] (1) The whitening and spot-lightening composition provided by the present invention, by adding various substances such as carnosine and oligopeptide-1, can effectively improve skin color problems such as spots and avoid the problem of dark spots;

[0029] (2) The whitening and spot-removing composition provided by the present invention can also provide moisture to the skin, maintain its supple luster, accelerate skin repair, and repair the skin from four dimensions: melanin, anti-sugar, anti-oxidation, and base repair. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1-2 This is a comparison chart of the VISA image results of two volunteers at 0 week and 4 week. DETAILED DESCRIPTION

[0031] The present invention is further explained below in conjunction with specific examples, but it should be noted that the following examples are only used to explain the present invention, and cannot be used to limit the present invention, and all technical solutions that are the same or similar to the present invention are within the scope of protection of the present invention. If no specific technology or conditions are specified in this embodiment, the operation is carried out according to the conventional technical methods and instrument instructions in the art; if the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially.

[0032] The glutathione (model Glutataione) can be purchased from Tokiwa Pharmaceutical Co., Ltd., and the carnosine (model Carnosine) can be purchased from Shenzhen Weiqi Pharmaceutical Research and Development Co., Ltd.; the tranexamic acid (model TRANEXAMICACID NK) can be purchased from Maruzen Pharmaceutical Co., Ltd.; the levorotatory VC (model ASCORBIC ACID ULTRA FINEPOWDER) and vitamin B12 (model Vitamin B12 Crystaline) can be purchased from DSM Nutritional Products Co., Ltd.; the oligopeptide-1 (model oligopeptide-1 liquid) can be purchased from Shanghai Haohai Biotechnology Co., Ltd.; the atelocollagen (INCI name ATELOCOLLAGEN, CAS No. 55963-88-7), the collagen is deer bone collagen (INCI name DEER BONE COLLAGEN), both of which can be purchased from Huzhou Purui Biopharmaceutical Technology Co., Ltd.; the nonapeptide-1 (model Whitenin Melanin Inhibitor Plus) can be purchased from Shenzhen Vicky Pharmaceutical Research and Development Co., Ltd.; the adenosine (model TeeCare Adeno) can be purchased from Guangzhou Tinglan Biotechnology Co., Ltd.; the DNA sodium (model RJMPDRN-M) can be purchased from Ruijiming (Shandong) Biotechnology Co., Ltd.; the sodium hyaluronate (model Hybloom HA-T) can be purchased from Huaxi Biotechnology Co., Ltd.; the sodium bisulfite (model sodium bisulfite) can be purchased from Xilong Science Co., Ltd.

[0033] Example 1 A mesotherapy composition for whitening and lightening spots

[0034] The mesotherapy composition for whitening and lightening spots consists of the following components and their mass percentages: 0.5% glutathione, 1% carnosine, 0.1% oligopeptide-1, 0.5% atelocollagen, 0.5% nonapeptide-1, 5% tranexamic acid, 0.1% L-VC, 0.1% vitamin B12, 2% adenosine, 0.05% sodium DNA, 0.1% sodium hyaluronate (molecular weight 500KDa), 0.1% sodium bisulfite, and sterile saline is added to 100%.

[0035] The preparation method of the mesotherapy composition for whitening and lightening spots is as follows:

[0036] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0037] S2, adding sodium hyaluronate to 2 / 3 of sterile physiological saline, heating to 80°C, keeping warm for 30 minutes, and stirring continuously at 50 rpm for 15 minutes until the above components are completely dissolved and transparent, to obtain mixture I;

[0038] S3, add sodium DNA to 1 / 3 sterile physiological saline, heat to 40°C, keep warm for 20 minutes, and stir continuously at 50 rpm for 20 minutes until the above components are completely dissolved and transparent, to obtain mixture II for use;

[0039] S4, lowering the temperature of the mixture I obtained in step S2 to 40°C, then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and stirring at 50 rpm for 30 min while cooling until the mixture is uniformly stirred, and finally the temperature is lowered to 25°C, and then the mixture II obtained in step S3 is added, and the mixture is fully mixed to obtain a composition I;

[0040] S5. Glutathione needs to be added to the composition I obtained in step S3 when the mesoderm treatment introduction operation is performed, and it should be prepared and used within 30 minutes.

[0041] Example 2 A mesotherapy composition for whitening and lightening spots

[0042] The mesotherapy composition for whitening and lightening spots is composed of the following components and their mass percentages: 5% glutathione, 0.5% carnosine, 0.01% oligopeptide-1, 0.1% atelocollagen, 0.01% nonapeptide-1, 5% tranexamic acid, 5% L-VC, 2% vitamin B12, 0.1% adenosine, 0.01% sodium DNA, 0.1% sodium hyaluronate (molecular weight 1000KDa), 0.01% sodium bisulfite, and sterile saline is added to 100%.

[0043] The preparation method of the mesotherapy composition for whitening and lightening spots is as follows:

[0044] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0045] S2, adding sodium hyaluronate to 2 / 3 of sterile physiological saline, heating to 90°C, keeping warm for 40 minutes, and stirring continuously at 100 rpm for 10 minutes until the above components are completely dissolved and transparent, to obtain mixture I;

[0046] S3, add sodium DNA to 1 / 3 sterile physiological saline, heat to 50°C, keep warm for 20 minutes, and stir continuously at 100 rpm for 10 minutes until the above components are completely dissolved and transparent, to obtain mixture II for use;

[0047] S4, lowering the temperature of the mixture I obtained in step S2 to 50°C, then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and stirring at 80 rpm for 30 min while cooling until the mixture is evenly stirred, and finally the temperature is lowered to 25°C, and then the mixture II obtained in step S3 is added, and the mixture is thoroughly mixed to obtain a composition I;

[0048] S5. Glutathione needs to be added to the composition I obtained in step S3 when the mesoderm treatment introduction operation is performed, and it should be prepared and used within 30 minutes.

[0049] Example 3 A mesotherapy composition for whitening and lightening spots

[0050] The mesotherapy composition for whitening and lightening spots is composed of the following components and their mass percentages: 3% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% atelocollagen, 0.3% nonapeptide-1, 3% tranexamic acid, 3% L-VC, 1% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate (molecular weight 800KDa), 0.3% sodium bisulfite, and sterile saline is added to 100%.

[0051] The preparation method of the mesotherapy composition for whitening and lightening spots is as follows:

[0052] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0053] S2, adding sodium hyaluronate to 2 / 3 sterile physiological saline, heating to 85°C, keeping warm for 35 minutes, and stirring continuously at 80 rpm for 15 minutes, until the above components are completely dissolved and transparent, to obtain mixture I;

[0054] S3, add sodium DNA to 1 / 3 sterile physiological saline, heat to 45°C, keep warm for 15 minutes, and stir continuously at 80 rpm for 15 minutes until the above components are completely dissolved and transparent, to obtain mixture II for use;

[0055] S4, lowering the temperature of the mixture I obtained in step S2 to 45°C, then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and stirring at 80 rpm for 25 min while cooling until the mixture is evenly stirred, and finally the temperature is lowered to 25°C, and then the mixture II obtained in step S3 is added, and the mixture is thoroughly mixed to obtain a composition I;

[0056] S5. Glutathione needs to be added to the composition I obtained in step S3 when the mesoderm treatment introduction operation is performed, and it should be prepared and used within 30 minutes.

[0057] Example 4 A mesotherapy composition for whitening and lightening spots

[0058] The mesotherapy composition for whitening and lightening spots consists of the following components and their mass percentages: 2% glutathione, 1% carnosine, 0.05% oligopeptide-1, 0.3% atelocollagen, 0.2% nonapeptide-1, 2% tranexamic acid, 3% L-VC, 0.8% vitamin B12, 1.2% adenosine, 0.1% sodium DNA, 0.6% sodium hyaluronate (molecular weight 1800KDa), 0.2% sodium bisulfite, and sterile saline is added to 100%.

[0059] The preparation method of the mesotherapy composition for whitening and lightening spots is as follows:

[0060] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0061] S2, adding sodium hyaluronate to 2 / 3 of sterile physiological saline, heating to 85°C, keeping warm for 40 minutes, and stirring continuously at 50 rpm for 15 minutes, until the above components are completely dissolved and transparent, to obtain mixture I;

[0062] S3, add sodium DNA to 1 / 3 sterile physiological saline, heat to 45°C, keep warm for 15 minutes, and stir continuously at 80 rpm for 10 minutes until the above components are completely dissolved and transparent, to obtain mixture II for use;

[0063] S4, lowering the temperature of the mixture I obtained in step S2 to 40-50° C., then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and stirring at 100 rpm for 30 min while cooling until the mixture is evenly stirred, finally cooling to 25° C., adding the mixture II obtained in step S3, and mixing thoroughly to obtain a composition I;

[0064] S5. Glutathione needs to be added to the composition I obtained in step S3 when the mesoderm treatment introduction operation is performed, and it should be prepared and used within 30 minutes.

[0065] Example 5 A mesotherapy composition for whitening and lightening spots

[0066] The mesotherapy composition for whitening and lightening spots is composed of the following components and their mass percentages: 0.4% glutathione, 2% carnosine, 0.2% oligopeptide-1, 0.4% atelocollagen, 0.5% nonapeptide-1, 4% tranexamic acid, 2% L-VC, 2% vitamin B12, 0.8% adenosine, 0.12% sodium DNA, 1% sodium hyaluronate (molecular weight 1000KDa), 0.5% sodium bisulfite, and sterile saline is added to 100%.

[0067] The preparation method of the mesotherapy composition for whitening and lightening spots is as follows:

[0068] S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside;

[0069] S2, adding sodium hyaluronate to 2 / 3 of sterile physiological saline, heating to 87°C, keeping warm for 34 minutes, and stirring continuously at 60 rpm for 20 minutes, until the above components are completely dissolved and transparent, to obtain mixture I;

[0070] S3, add sodium DNA to 1 / 3 sterile physiological saline, heat to 43°C, keep warm for 20 minutes, and stir continuously at 80 rpm for 20 minutes until the above components are completely dissolved and transparent, to obtain mixture II for use;

[0071] S4, lowering the temperature of the mixture I obtained in step S2 to 43°C, and then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and stirring at 100 rpm for 30 min while cooling, until the mixture is evenly stirred, and finally cooling to 25°C, and then adding the mixture II obtained in step S3, and mixing thoroughly to obtain a composition I;

[0072] S5. Glutathione needs to be added to the composition I obtained in step S3 when the mesoderm treatment introduction operation is performed, and it should be prepared and used within 30 minutes.

[0073] Comparative Example 1 A composition for mesotherapy

[0074] The composition of the mesotherapy is composed of the following components and their mass percentages: 6% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% atelocollagen, 0.3% nonapeptide-1, 3% tranexamic acid, 1% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate (molecular weight 800KDa), 0.3% sodium bisulfite, and sterile saline is added to 100%.

[0075] The preparation method of the mesotherapy composition is similar to that of Example 3.

[0076] Comparative Example 2 A mesotherapy composition

[0077] The composition of the mesotherapy is composed of the following components and their mass percentages: 3% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% atelocollagen, 3% tranexamic acid, 3% L-VC, 1% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate (molecular weight 800KDa), 0.3% sodium bisulfite, and sterile saline is added to 100%.

[0078] The preparation method of the mesotherapy composition is similar to that of Example 3.

[0079] Comparative Example 3 A composition for mesodermal therapy

[0080] The mesotherapy composition is composed of the following components and their mass percentages: 3% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% collagen, 0.3% nonapeptide-1, 3% tranexamic acid, 3% L-VC, 22% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate (molecular weight 800KDa), 0.3% sodium bisulfite, and sterile saline is added to 100%.

[0081] The preparation method of the mesotherapy composition is similar to that of Example 3.

[0082] Test example: Human efficacy test

[0083] 1. Test samples: compositions prepared in Examples 1-5 and Comparative Examples 1-3 of the present invention;

[0084] 2. Experimental subjects: 160 healthy male and female volunteers aged 25 to 60 were selected and divided into 8 groups, with 20 subjects in each group;

[0085] Requirements: Applicants must have uneven skin color such as dark yellow, spots (chloasma, sun spots, dark acne marks) on their face; no allergic diseases, no history of allergies to cosmetics and other topical preparations; no history of photosensitivity diseases, and no recent use of drugs that affect photosensitivity; able to understand the trial process and voluntarily participate in the trial.

[0086] Exclusion criteria: patients with severe skin infection; patients with severe sensitive skin; patients with hormonal dermatitis; patients with allergies to the drugs in the formula; pregnant women; patients with mental disorders; patients with severe cardiovascular, liver, and kidney diseases;

[0087] 3. Test process: The trial part is the face. Wash the face before the test and take VISA. Apply 10 grams of 5% compound lidocaine cream for 30 minutes and clean it up. Disinfect with iodine 2 times and deiodine with saline 2 times. Then use Haifei 6th generation water light instrument (nine needles) to inject the composition of embodiment 1 to 5 and comparative example 1 to 3 groups into the dermis of each group of volunteers (needle insertion depth 0.8mm-1.5mm), wherein the composition needs to be prepared on the spot, and 2mL is injected into the whole face. Immediately after the operation, apply 5℃ medical sterile cold compress to the face for 25min. Keep the face clean and sterile within 48 hours after the operation, and wipe the face with saline instead of raw water.

[0088] According to the VISA photo evaluation, the improvement of skin color is divided into five levels: obvious improvement, relatively obvious improvement, no obvious improvement, relatively obvious deterioration, and obvious deterioration. According to the comfort of injection, the product is divided into five levels: no pain, slight pain, moderate pain, relatively painful but tolerable, and unbearable pain. Follow-up visits were conducted after 1 week, 2 weeks, and 4 weeks, and VISA photos were taken after washing the face. The improvement of skin color was compared with the VISA photos taken before the injection, and volunteers were asked to self-evaluate their comfort.

[0089] 4. Test results: Skin color improvement is shown in Table 1, comfort self-evaluation results are shown in Table 2, and some VISA pictures are shown in Figure 1-2 .

[0090] Table 1 Skin color improvement

[0091]

[0092]

[0093] Table 2 Volunteer comfort self-evaluation form

[0094] Experimental Group Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 total No pain / person 12 10 15 15 13 13 13 12 103 Slight tingling sensation / person 6 8 5 4 6 5 6 6 46 Moderate stinging pain / person 2 2 0 1 1 2 1 2 11 Painful but bearable / person 0 0 0 0 0 0 0 0 0 Unbearable pain person 0 0 0 0 0 0 0 0 0

[0095] From Table 1 and Table 2 above, Figure 1 and Figure 2 It can be seen that the skin color of the volunteers injected with the composition prepared in Examples 1-5 of the present invention was improved to varying degrees: the effectiveness of the mesotherapy composition provided by the present invention in improving skin color / spots for one week was 94% (the ratio of the number of improved people in 5 groups to the total number of people), the satisfaction with comfort was 93.13%, and no adverse reactions related to the product occurred, with good safety.

[0096] In comparison, the comparative example 1 of the present invention does not contain L-VC, but increases the content of glutathione. The comparative example 2 does not contain nonapeptide-1 to ensure that the effective ingredients of the composition remain unchanged. The comparative example 3 replaces the terminal collagen with conventional collagen. As a result, the number of effective people with improved skin color is significantly lower than that of the examples 1-5. This shows that each component in the examples of the present invention is necessary and irreplaceable even if they have the same function in the composition.

[0097] Finally, it should be noted that the above embodiments are only illustrative of the principles, performance and efficacy of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A mesotherapy composition for whitening and lightening spots, characterized in that: The invention is composed of the following components and their mass percentages: 0.5-5% glutathione, 0.5-4% carnosine, 0.01-0.2% oligopeptide-1, 0.1-0.5% atelocollagen, 0.01-0.5% nonapeptide-1, 0.5-5% tranexamic acid, 0.1-5% L-VC, 0.1-2% vitamin B12, 0.1-2% adenosine, 0.01-0.2% sodium DNA, 0.1-1% sodium hyaluronate, 0.01-0.5% sodium bisulfite and 74.1-97.96% sterile saline.

2. The composition according to claim 1, characterized in that It is composed of the following components and their mass percentages: 3% glutathione, 2% carnosine, 0.1% oligopeptide-1, 0.3% atelocollagen, 0.3% nonapeptide-1, 3% tranexamic acid, 3% L-VC, 1% vitamin B12, 1% adenosine, 0.1% sodium DNA, 0.5% sodium hyaluronate, 0.3% sodium bisulfite and 85.4% sterile saline.

3. The composition according to claim 1 or 2, characterized in that The sodium DNA is polydeoxyribonucleotide, and the extraction process is as follows: sodium DNA is extracted from salmon sperm DNA, and then derived through controlled purification and sterilization.

4. The composition according to claim 1 or 2, characterized in that The molecular weight of the sodium hyaluronate is selected from 500-800 KDa and 1000-1800 KDa.

5. A method for preparing the composition according to any one of claims 1 to 4, characterized in that: The specific preparation process is as follows: S1. Weigh glutathione, carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine, sodium DNA, sodium hyaluronate, sodium bisulfite and sterile saline respectively according to mass percentage and set aside; S2. Add sodium hyaluronate to 2 / 3 of sterile physiological saline, heat to 80-90° C., keep warm for 30-40 minutes, and stir continuously until the above components are completely dissolved and transparent, to obtain mixture I; S3. Add sodium DNA to 1 / 3 sterile saline and heat to 40-50°C for 10-20 minutes with constant stirring until the above components are completely dissolved and transparent to obtain mixture II for later use; S4, lowering the temperature of the mixture I obtained in step S2 to 40-50° C., then sequentially adding carnosine, oligopeptide-1, atelocollagen, nonapeptide-1, tranexamic acid, L-VC, vitamin B12, adenosine and sodium bisulfite, and starting to cool and stir until uniformly stirred, then adding the mixture II obtained in step S3, and mixing thoroughly to obtain a composition I; S5. Add glutathione to the composition I obtained in step S3, and mix well to obtain.

6. The preparation method according to claim 5, characterized in that: The continuous stirring in steps S2 and S3 is to stir slowly for 10 to 20 minutes at a rotation speed of 50 to 100 rpm.

7. The preparation method according to claim 5, characterized in that: The cooling and stirring in step S4 is to gradually cool the mixture to 25° C. and stir the mixture at a speed of 50 to 100 rpm for 20 to 30 minutes.

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