Optimized plasma matrix and preparation method thereof
Near infrared irradiation activates the coagulation cascade in the plasma matrix, forming a dense fiber network, solving the problem of insufficient strength and biological activity of the plasma matrix, and achieving more ideal therapeutic effects and tissue regeneration effects.
Patent Information
- Application Number
- CN202411486077.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-23
- Publication Date
- 2025-01-07
AI Technical Summary
The existing plasma matrix has insufficient strength and biological activity during application, which makes it difficult to effectively promote wound healing.
Near infrared rays are used to irradiate the plasma matrix, activate the coagulation cascade, form a dense three-dimensional fiber network structure, improve its strength and degradation time, and induce the synthesis of collagen and elastin, enhancing the tissue regeneration effect.
It significantly improves the strength and biological activity of the plasma matrix, extends the degradation time, promotes the proliferation and migration and differentiation of fibroblasts, improves the wound healing effect, and has broad application prospects.
Smart Images
Figure CN119258095A_ABST
Abstract
Description
Technical Field
[0001] The present disclosure relates to the technical field of blood products, and in particular to an optimized plasma matrix and a preparation method thereof. Background Art
[0002] Wound healing is a complex biological process, which mainly includes four periods: bleeding period, inflammatory period, proliferation period and reconstruction period. In these processes, platelet aggregation is a very critical link. Platelet concentrate products that promote wound healing by enriching supraphysiological doses of platelets have come into being. With the development of technology and economy, the development and evolution of plasma matrix has been decades. Platelet concentrates have been widely used as a source of regenerative autologous growth factors in soft and hard tissue regeneration, facial beauty, hair regeneration and other therapeutic fields. However, the plasma matrix still has defects such as insufficient strength and biological activity during application, which needs to be further improved.
[0003] Therefore, how to improve the strength and biological activity of the existing plasma matrix has become a problem that needs to be solved urgently. Summary of the invention
[0004] In order to solve the above technical problems, the present disclosure provides an optimized plasma matrix and a preparation method thereof. The plasma matrix prepared by the preparation method has higher strength and longer degradation time, and can effectively promote fibroblast proliferation, migration and differentiation, and promote wound healing, and has broad application prospects.
[0005] In a first aspect, the present disclosure provides a method for preparing an optimized plasma matrix, comprising the following steps:
[0006] (1) obtaining a whole blood sample and obtaining a plasma matrix after centrifugation;
[0007] (2) irradiating the plasma matrix obtained in step (1) with near infrared rays to obtain an optimized plasma matrix;
[0008] The wavelength of the near infrared ray is 780-2500nm.
[0009] The wavelength of the near infrared ray may be 780 nm, 800 nm, 900 nm, 1000 nm, 1500 nm, 2000 nm or 2500 nm, but is not limited to the listed values, and other values not listed within the range are also applicable.
[0010] Infrared (IR) is an electromagnetic wave with a wavelength between microwaves and visible light. The energy it carries can be absorbed by molecules in the body, causing them to move faster and produce a "heating effect". Infrared is divided into near infrared (wavelength below 2.5μm) and far infrared (wavelength above 15μm).
[0011] The present invention utilizes the thermal effect of near infrared rays to efficiently activate the coagulation cascade reaction, promote the formation of a three-dimensional fiber network structure inside the plasma matrix, and make its structure denser, so that it has better tensile strength and longer degradation time, thereby achieving a more ideal therapeutic effect. At the same time, near infrared irradiation can also induce the synthesis of collagen and elastin, achieving a better effect of promoting tissue regeneration.
[0012] The following are preferred technical solutions of the present disclosure, but are not intended to be limitations of the technical solutions provided by the present disclosure. Through the following technical solutions, the technical objectives and beneficial effects of the present disclosure can be better achieved and realized.
[0013] As a preferred technical solution of the present disclosure, the centrifugal force of the centrifugation in step (1) is 60-1000×g, for example, 60×g, 100×g, 200×g, 300×g, 500×g, 700×g or 1000×g, but is not limited to the listed values, and other values not listed within the range are also applicable.
[0014] As a preferred technical solution of the present invention, the centrifugation time in step (1) is 2-10 min, for example, 2 min, 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min or 10 min, but is not limited to the listed values, and other values not listed within the range are also applicable.
[0015] As a preferred technical solution of the present disclosure, the plasma matrix obtained in step (1) includes a liquid plasma matrix or a solid plasma matrix.
[0016] As a preferred technical solution of the present disclosure, the preparation method further comprises: before performing step (2), mixing the obtained plasma matrix with a material having near-infrared sensitivity to form a composite material.
[0017] Preferably, the near-infrared sensitive material includes one or more of gold nanoparticles, graphene, black phosphorus or carbon nanotubes, such as a combination of graphene and carbon nanotubes, a combination of gold nanoparticles and graphene, etc.
[0018] Preferably, the added mass of the near-infrared sensitive material does not exceed 10% of the total mass of the near-infrared sensitive material and the plasma matrix, for example 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, etc., but is not limited to the listed values, and other unlisted values within the range are also applicable.
[0019] For the liquid plasma matrix, the preparation method disclosed in the present invention can promote its rapid coagulation, so that it has a certain retention force. Furthermore, when the liquid plasma matrix is mixed with auxiliary materials (materials with near-infrared sensitivity) and then irradiated with near-infrared rays, the auxiliary materials can be distributed more evenly and have greater strength.
[0020] As a preferred technical solution of the present disclosure, the preparation method further comprises: before performing step (2), preparing the obtained plasma matrix into a plasma matrix product.
[0021] As a preferred technical solution of the present disclosure, the preparation method further comprises: before carrying out step (2), preparing the obtained composite material into a plasma matrix product.
[0022] As a preferred technical solution of the present disclosure, the plasma matrix product includes any one of a plasma matrix membrane, a plasma matrix plug or a plasma matrix bone block.
[0023] As a preferred technical solution of the present invention, the radiation power of the near infrared ray in step (2) is 0.2-3W, for example, 0.2W, 0.5W, 1W, 1.5W, 2W, 2.5W or 3W, but is not limited to the listed values, and other unlisted values within the range are also applicable.
[0024] Preferably, the near-infrared irradiation time in step (2) is 15-30 min, such as 15 min, 20 min, 25 min or 30 min, but is not limited to the listed values, and other values not listed within the range are also applicable.
[0025] In the present disclosure, selecting appropriate radiation power and irradiation time helps to further enhance the performance of the plasma matrix. If the radiation power is too large or the irradiation time is too long, it will cause the fibrin structure inside the plasma matrix to denature, water loss, cell activity to decrease, mechanical strength and tensile properties to decrease, which is not conducive to being used as a barrier material or bone replacement material.
[0026] Preferably, the near infrared irradiation temperature in step (2) does not exceed 60°C, for example, 20°C, 30°C, 40°C, 50°C or 60°C, but is not limited to the listed values, and other unlisted values within the range are also applicable.
[0027] In a second aspect, the present disclosure provides an optimized plasma matrix prepared by the preparation method described in the first aspect.
[0028] In a third aspect, the present disclosure provides an application of the optimized plasma matrix as described in the second aspect, wherein the optimized plasma matrix is used in the fields of soft and hard tissue regeneration, facial beauty, hair regeneration, refractory wound healing, and osteoarthritis treatment.
[0029] Specifically, it can be used to:
[0030] (1) It can better maintain the mucosal contour during soft tissue augmentation and resist the negative impact of complex chewing movements on the reconstructed area during gingival papilla reconstruction;
[0031] (2) guiding longer degradation time in bone tissue regeneration;
[0032] (3) provide stronger mechanical protection for the bone augmentation area;
[0033] (4) Better stretchability enables the membrane to be applied to defective areas with complex surface morphology.
[0034] It should be noted that regarding the timing of near-infrared irradiation in the present invention, (1) the obtained plasma matrix can be directly irradiated with near-infrared rays, and then the obtained optimized plasma matrix is applied to the desired treatment site; or, (2) the obtained plasma matrix is first applied to the desired treatment site, and then irradiated with near-infrared rays to form an optimized plasma matrix.
[0035] Compared with the prior art, the technical solution provided by the embodiments of the present disclosure has the following advantages:
[0036] (1) The preparation method disclosed in the present invention uses near-infrared rays to irradiate the plasma matrix to induce temperature rise, thereby activating platelets in the plasma matrix and causing fibrin deformation, forming a larger and more solid fibrin matrix network, thereby encapsulating more platelets, leukocytes and growth factors, prolonging the release time of growth factors, and promoting biological activity.
[0037] (2) The preparation method disclosed in the present invention uses near-infrared rays to irradiate the plasma matrix to increase the proliferation, migration and differentiation of fibroblasts. In practical applications, the combination of the plasma matrix three-dimensional scaffold and internal cells or growth factors can achieve better tissue healing effects. BRIEF DESCRIPTION OF THE DRAWINGS
[0038] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the principles of the present disclosure.
[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.
[0040] Figure 1 This is a SEM image of the plasma matrix membrane described in Comparative Example 1 of the present disclosure.
[0041] Figure 2 This is a SEM image of the optimized plasma matrix described in Example 1 of the present disclosure.
[0042] Figure 3 This is a SEM image of the optimized plasma matrix described in Example 2 of the present disclosure.
[0043] Figure 4 This is a graph showing the strain results of the tensile test of the plasma matrix membrane described in Comparative Example 1 of the present disclosure and the optimized plasma matrix described in Examples 1-2.
[0044] Figure 5 This is a graph showing stress results of tensile tests on the plasma matrix membrane described in Comparative Example 1 of the present disclosure and the optimized plasma matrix described in Examples 1-2.
[0045] Figure 6 This is a graph showing the temperature changes of the optimized plasma matrix during near-infrared irradiation as described in Example 1 of the present disclosure.
[0046] Figure 7 This is a graph showing the temperature changes of the optimized plasma matrix during near-infrared irradiation as described in Example 2 of the present disclosure. DETAILED DESCRIPTION
[0047] In order to more clearly understand the above-mentioned objectives, features and advantages of the present disclosure, the scheme of the present disclosure will be further described below. It should be noted that the embodiments of the present disclosure and the features in the embodiments can be combined with each other without conflict.
[0048] In the following description, many specific details are set forth to facilitate a full understanding of the present disclosure, but the present disclosure may also be implemented in other ways different from those described herein; it is obvious that the embodiments in the specification are only part of the embodiments of the present disclosure, rather than all of the embodiments.
[0049] Example 1
[0050] This embodiment provides a method for preparing an optimized plasma matrix, the preparation method comprising the following steps:
[0051] (1) 10 mL of blood was collected from the volunteer's elbow using a hydrophilic blood collection tube, and then quickly placed in a horizontal centrifuge and centrifuged at 700 × g for 8 min to obtain a solid plasma matrix;
[0052] (2) pressing the solid plasma matrix obtained in step (1) into a solid plasma matrix membrane using a plasma matrix membrane pressing device kit at a pressure of 7 N for 60 seconds;
[0053] The obtained solid plasma matrix membrane was irradiated with near infrared light of 2 W and wavelength of 1500 nm for 27 minutes to obtain an optimized plasma matrix.
[0054] Example 2
[0055] This embodiment provides a method for preparing an optimized plasma matrix, the preparation method comprising the following steps:
[0056] (1) 10 mL of blood was collected from the volunteer's elbow using a hydrophobic blood collection tube, and then quickly placed in a horizontal centrifuge and centrifuged at 700 × g for 8 min to obtain liquid plasma matrix;
[0057] (2) The liquid plasma matrix obtained in step (1) is placed in an EP tube and irradiated with near-infrared light with a power of 2 W and a wavelength of 1500 nm for 20 minutes to obtain an optimized plasma matrix.
[0058] Example 3
[0059] This embodiment provides a method for preparing an optimized plasma matrix, the preparation method comprising the following steps:
[0060] (1) 10 mL of blood was collected from the volunteer's elbow using a hydrophilic blood collection tube, and then quickly placed in a horizontal centrifuge and centrifuged at 700 × g for 8 min to obtain liquid plasma matrix;
[0061] (2) 2 g of the liquid plasma matrix obtained in step (1) was mixed with 0.2 g of carbon nanotubes (diameter 20 nm) and placed in an EP tube, and irradiated with near-infrared light with a power of 2 W and a wavelength of 1500 nm for 20 min to obtain an optimized plasma matrix.
[0062] Example 4
[0063] This embodiment provides an optimized method for preparing a plasma matrix. The preparation method is based on the method of embodiment 1, with the only difference being that in step (2), the power of the near infrared ray is 3 W, the wavelength is 2500 nm, and the irradiation time is 15 min.
[0064] Comparative Example 1
[0065] This comparative example provides a plasma matrix membrane, which is obtained by the same method as in Example 1, but without near-infrared irradiation.
[0066] Performance Test:
[0067] (I) The plasma matrix membrane of comparative example 1 without stretching treatment and the optimized plasma matrix obtained in examples 1-2 were placed in a glutaraldehyde solution with a concentration of 2.5wt% for 4 hours, dehydrated with gradient alcohol, freeze-dried at -51°C, and gold-sprayed using an ISC150 ion sputtering instrument (operating conditions: ultimate vacuum <1Pa; working pressure 4Pa; rotation speed: 60rpm; target substrate distance 40mm; sputtering power: Max.20W, Max.100mA, 120V) and then observed by scanning electron microscopy. The results are as follows: Figure 1-Figure 3 shown.
[0068] from Figure 1-Figure 3 It can be seen that compared with Comparative Example 1, the plasma matrix fibrin network irradiated by near infrared rays in Example 1 and Example 2 is more dense and the porosity is reduced. A denser fibrin network helps to improve the mechanical properties of the plasma matrix membrane and prolong the degradation time, thereby achieving a better support effect in guided tissue regeneration therapy.
[0069] (ii) The plasma matrix membrane described in Comparative Example 1 and the reinforced solid plasma matrix membrane obtained in Example 1-2 were respectively measured for their tensile properties using a tensile tester (model: ZQ-990, purchased from Dongguan Zhiqu Precision Instrument Co., Ltd.). The maximum load capacity of the tensile tester was set to 5N when the standard tensile rate was 1mm / min. Before the tensile test, the original length between the two clamps was set to 5mm. After the tensile test, the strain results were as follows: Figure 4 The stress results are shown in Figure 5 shown.
[0070] Depend on Figure 4 and Figure 5 It can be seen that the plasma matrix after near-infrared irradiation has enhanced tensile strain and tensile stress, enabling it to better resist the stretching and tearing of external mechanical stress, and has broader application prospects in clinical practice.
[0071] (III) Determining the temperature change of the optimized plasma matrix described in Example 1-2 during near-infrared irradiation, the results are as follows: Figure 6-Figure 7 shown.
[0072] Depend on Figure 6 and Figure 7 It can be seen that under near-infrared treatment, the internal temperature of the plasma matrix mixture gradually rises, the temperature rise process is relatively gentle, and the maximum temperature does not exceed 60°C, which helps to protect the internal cell activity.
[0073] It should be noted that, in this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0074] The above description is only a specific embodiment of the present disclosure, so that those skilled in the art can understand or implement the present disclosure. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure will not be limited to the embodiments described herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for preparing an optimized plasma matrix, characterized in that: The preparation method comprises the following steps: (1) obtaining a whole blood sample and obtaining a plasma matrix after centrifugation; (2) irradiating the plasma matrix obtained in step (1) with near infrared rays to obtain an optimized plasma matrix; The wavelength of the near infrared ray is 780-2500nm.
2. The preparation method according to claim 1, characterized in that: The centrifugal force of the centrifugation in step (1) is 60-1000×g.
3. The preparation method according to claim 1 or 2, characterized in that: The centrifugation time in step (1) is 2-10 min.
4. The preparation method according to any one of claims 1 to 3, characterized in that The plasma matrix obtained in step (1) includes a liquid plasma matrix or a solid plasma matrix.
5. The preparation method according to any one of claims 1 to 4, characterized in that: The preparation method further comprises: before carrying out step (2), mixing the obtained plasma matrix with a material having near infrared sensitivity to form a composite material; Preferably, the near-infrared sensitive material includes one or more of gold nanoparticles, graphene, black phosphorus or carbon nanotubes; Preferably, the added mass of the near-infrared-sensitive material does not exceed 10% of the total mass of the near-infrared-sensitive material and the plasma matrix.
6. The preparation method according to any one of claims 1 to 4, characterized in that: The preparation method further comprises: before carrying out step (2), preparing the obtained plasma matrix into a plasma matrix product.
7. The preparation method according to claim 5, characterized in that: The preparation method further comprises: before carrying out step (2), preparing the obtained composite material into a plasma matrix product.
8. The preparation method according to claim 6 or 7, characterized in that: The plasma matrix product includes any one of a plasma matrix membrane, a plasma matrix plug or a plasma matrix bone block.
9. The preparation method according to any one of claims 1 to 8, characterized in that: The near infrared radiation power of step (2) is 0.2-3W; Preferably, the near-infrared irradiation time in step (2) is 15-30 minutes; Preferably, the near infrared irradiation temperature in step (2) does not exceed 60°C.
10. An optimized plasma matrix, characterized in that The optimized plasma matrix is prepared by the preparation method according to any one of claims 1-9.