Traditional Chinese medicine composition capable of recovering energy, restoring consciousness and resisting fatigue and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition capable of restoring energy, restoring consciousness and resisting fatigue and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials: radix astragali preparata, fructus lycii, herba ecliptae, fructus ligustri lucidi, radix polygalae, rhizoma chuanxiong, American ginseng, rhizoma acori graminei and herba menthae, has the effects of tonifying spleen and qi, soothing liver and regulating qi, tonifying liver and kidney, inducing resuscitation and tranquilizing mind, and is used for treating body fatigue caused by liver depression and spleen deficiency, liver and kidney deficiency and liver-spleen disharmony; animal experiment results show that the traditional Chinese medicine composition has the effects of enhancing exercise ability and muscle endurance ability, improving attention and memory and enhancing immunity, and can effectively improve the fatigue state. The traditional Chinese medicine composition disclosed by the invention can be used for developing anti-fatigue medicines and related health-care products.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicines, and relates to a traditional Chinese medicine composition with anti-fatigue effects of restoring energy and refreshing the brain, and specifically relates to a traditional Chinese medicine formula named "Shenqi Fu Neng Xingnao Fang" and a preparation method and use thereof. Background Art

[0002] With the rapid development of modern society, fatigue has become an increasingly prominent problem, seriously affecting production safety and personal physical and mental health. Fatigue is a complex sub-health state involving both physical and psychological aspects. Physical fatigue mainly stems from excessive mental and physical labor, circadian rhythm disorders and harsh working environments (such as long-term flights under high-altitude environmental pressure), and manifests as fatigue states such as muscle soreness, fatigue and slow reaction; psychological fatigue stems from high concentration, complex decision-making and stressful working environment, and manifests as fatigue states such as inattention, decreased judgment and memory loss. These fatigue states not only affect work performance, but long-term accumulation may also lead to more serious health problems, so effective prevention and relief measures are urgently needed.

[0003] At present, various industries (such as the aviation industry) have adopted measures to prevent and alleviate fatigue, mainly including: adjusting working hours, improving rest conditions, and taking health products or western medicine. However, these measures can only temporarily relieve symptoms, and the use of western medicine alone may bring side effects and is not suitable for long-term use. In contrast, traditional Chinese medicine has unique advantages in regulating sub-health status. According to traditional Chinese medicine theory, fatigue is related to liver depression and spleen deficiency, liver and kidney deficiency, and liver and spleen disharmony. Traditional Chinese medicine that replenishes qi and nourishes yin, strengthens the spleen and moistens the lungs, and refreshes the mind can be used for syndrome differentiation and treatment to improve physical and mental fatigue to a certain extent. For example, CN103404847A adopts the following Chinese medicine formula: icariin, astragaloside IV, polygonatum extract, and American ginseng extract, which have the effects of improving excessive mental and physical labor, improving memory, enhancing immunity, and delaying aging; CN107752119A adopts the following Chinese medicine formula: Gastrodia elata, Chuanxiong, Radix dasyphyllae, Uncaria rhynchophylla, Curcuma aromatica, Acorus calamus, and Polygala tenuifolia; it has the anti-fatigue effect of refreshing the mind; CN113712903A adopts the following Chinese medicine formula: Rhodiola rosea extract, Acorus calamus extract, peppermint oil, and borneol, and the dosage form is an external gel, which has the anti-fatigue effect of refreshing the mind and relieving physical fatigue; CN110663783A adopts the following Chinese medicine formula: Codonopsis pilosula, Astragalus membranaceus, Lycium barbarum, Schisandra chinensis, and Licorice, and is compounded with green tea, which has the anti-fatigue effect of improving immunity, refreshing the mind, and enhancing physical strength. However, the traditional Chinese medicine formulas of existing anti-fatigue products pay little attention to the protection of the liver and kidneys in terms of restoring and enhancing anti-fatigue capabilities. At the same time, they generally lack the preventive effect of delaying the occurrence of fatigue, and may also cause side effects or adverse reactions such as liver and kidney damage, indigestion or stomach pain.

[0004] In addition, the classic Chinese medicine pair "Ligustrum lucidum and Eclipta prostrata" has the effects of nourishing the liver and kidneys, nourishing yin and stopping bleeding, and can be used for vertigo and tinnitus caused by liver and kidney yin deficiency. Summary of the invention

[0005] The purpose of the present invention is to provide a Chinese medicine composition for restoring brain function and resisting fatigue and its use.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: In a first aspect, a traditional Chinese medicine composition with anti-fatigue effect is provided, which is made of the following traditional Chinese medicine raw materials in parts by weight: 20-50 parts of roasted astragalus or astragalus, 10-30 parts of wolfberry, 10-30 parts of Eclipta prostrata, 5-20 parts of Ligustrum lucidum, 5-20 parts of Polygala tenuifolia, 5-20 parts of Chuanxiong, 5-15 parts of American ginseng, 5-15 parts of Acorus calamus, and 4-15 parts of mint.

[0007] Preferably, the Chinese medicine composition is made of the following Chinese medicine raw materials in parts by weight: 25-35 parts of roasted astragalus or astragalus, 15-25 parts of wolfberry, 15-25 parts of Eclipta prostrata, 12-18 parts of Ligustrum lucidum, 12-18 parts of Polygala tenuifolia, 10-15 parts of Chuanxiong, 8-12 parts of American ginseng, 8-12 parts of Acorus calamus, and 5-8 parts of mint.

[0008] Preferably, the Chinese medicine composition is made of the following Chinese medicine raw materials in parts by weight: 30 parts of roasted astragalus or astragalus, 20 parts of wolfberry, 20 parts of Eclipta prostrata, 15 parts of Ligustrum lucidum, 15 parts of Polygala tenuifolia, 12 parts of Chuanxiong, 10 parts of American ginseng, 10 parts of Acorus calamus, and 6 parts of mint.

[0009] In a second aspect, a method for preparing the above-mentioned Chinese medicine composition having anti-fatigue effect is provided, comprising the following steps: The medicinal materials are weighed according to the formula ratio (i.e. the dosage of the above-mentioned Chinese medicinal raw materials), extracted with water, filtered (the filtrate is the extract), and the extracts are combined, concentrated, and spray-dried to obtain a dry paste powder of the Chinese medicinal composition.

[0010] Preferably, the extraction conditions include: heating and extracting with 5-20 times (e.g. 10 times) of water for 2-5 times (e.g. 3 times), and filtering after each extraction for 1-3 hours (e.g. 1 hour).

[0011] In a third aspect, the present invention provides use of the above-mentioned Chinese medicine composition with anti-fatigue effect in preparing products for preventing and relieving fatigue.

[0012] Preferably, the product is a medicine, a health product or a functional food.

[0013] Preferably, the product is used to relieve chronic fatigue.

[0014] Preferably, the product has the effect of enhancing athletic ability and muscle tolerance, and restoring physical energy (i.e., recovery).

[0015] Preferably, the product also has the effects of improving attention and memory, and enhancing immunity.

[0016] Preferably, the dosage form of the product is selected from any one of a traditional dosage form and a modern dosage form.

[0017] Preferably, taking pharmaceutical preparations as an example, the dosage form is any one of tablets, capsules, granules, pills, powders, pills, syrups, oral solutions, and oral suspensions.

[0018] Preferably, the pharmaceutical preparation contains 0.1%-90% of the active pharmaceutical ingredient (i.e. the above-mentioned Chinese medicine composition with anti-fatigue effect) by weight, and the remainder is excipients.

[0019] Preferably, the functional food is a beverage.

[0020] The beneficial effects of the present invention are embodied in: The Chinese medicine composition of the present invention comprises nine medicinal materials, namely, Eclipta prostrata, Ligustrum lucidum, American ginseng, roasted Astragalus membranaceus (or Astragalus), Lycium barbarum, Polygala tenuifolia, Ligusticum chuanxiong, Acorus calamus and mint, which are combined. The Chinese medicine composition has application safety and can better exert anti-fatigue effects (enhancing muscle tolerance, enhancing exercise ability, restoring physical fitness, improving physical and mental fatigue, enhancing immunity, and improving attention and memory), thereby effectively avoiding accidents caused by related factors such as excessive fatigue. The Chinese medicine composition has high application value in improving (relieving) the fatigue state of people who overwork mentally and physically (such as workers in the aviation industry and other industries), developing preventive drugs and related health care and functional products.

[0021] Furthermore, the present invention also increases the dosage of roasted astragalus and wolfberry (compared with other medicinal flavors) so that the Chinese medicine composition can enhance exercise capacity and fatigue tolerance (such as enhancing muscle tolerance), delay the body's consumption of glycogen and inhibit excessive accumulation of metabolites, enhance the level of antioxidant enzymes in the body, inhibit lipid peroxidation and reduce cell damage, relieve muscle fiber contraction and increase the number of Nissl bodies in the hippocampus, increase the number of mitochondria in the gastrocnemius and hippocampus, and improve mitochondrial pathological morphology. Therefore, related products (such as drugs with the Chinese medicine composition as an active ingredient) can enhance exercise capacity by regulating muscle and brain metabolism levels, thereby playing a role in preventing fatigue and relieving chronic fatigue, thereby significantly enhancing the body's anti-fatigue ability.

[0022] Furthermore, the present invention increases the medicinal pair of "Ligustrum lucidum and Eclipta prostrata" and controls the ratio of the two medicinal flavors in the medicinal pair, and makes a more reasonable combination with other medicinal flavors (such as Lycium barbarum), thereby enhancing the effect of the traditional Chinese medicine composition in nourishing the liver and kidneys, and avoiding the risk of discomfort such as dizziness after taking the medicine by protecting the liver and kidneys. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The improvement effect of the Chinese medicine composition (specifically SQFNXNP) on the behavior of fatigue mice: A. Acute and chronic fatigue experimental process (involving the modeling procedure of the chronic fatigue mouse model and the drug administration process of each experiment); B. Schematic diagram of behavioral investigation; C. Exhaustive swimming time of mice with acute fatigue; D. Exhaustive swimming time of mice with chronic fatigue; E. Immobility time of mice in the open field test (OFT); F. Total moving distance of mice in OFT; G. Heat map of activity trajectories of mice in OFT; H. Swimming time of mice in the forced swimming test (FST); I. Immobility time of mice in FST; J. Struggling time of mice in the tail suspension test (TST); K. Immobility time of mice in TST; # p <0.05 or ## p <0.01 vs Normal control group (Control), * p <0.05 or ** p <0.01 vs Model group (Model).

[0024] Figure 2 The improvement effect of the Chinese medicine composition (specifically SQFNXNP) on the biochemical indices in fatigue mice: A. Creatine kinase (CK); B. Liver glycogen; C. Muscle glycogen; D. Lactate (LD); E. Lactate dehydrogenase (LDH); F. Blood urea nitrogen (BUN); G. Superoxide dismutase (SOD); H. Malondialdehyde (MDA); I. Glutathione peroxidase (GSH-PX); J. Catalase (CAT); # p <0.05 or ## p <0.01 vs Normal control group (Control), * p <0.05 or ** p <0.01 vs Model group (Model).

[0025] Figure 3 The improvement effect of the Chinese herbal composition (specifically SQFNXNP) on the pathological morphology of the gastrocnemius muscle of fatigued mice: A. H&E staining of mouse gastrocnemius muscle tissue sections, magnifications of 10× and 40×, scale bar (black) 100 μm, scale bar (red) 200 μm; B. Transmission electron microscopy (TEM) image of mouse gastrocnemius muscle tissue, scale bar (black) 2 μm, scale bar (red) 500 nm.

[0026] Figure 4 The improvement effect of the Chinese herbal composition (specifically SQFNXNP) on the pathological morphology of the hippocampus in fatigue mice: A. Nissl staining of the hippocampus in the mouse brain, magnifications of 10× and 40×, scale (black) 100 μm, scale (red) 20 μm; B. Transmission electron microscopy (TEM) image of mitochondria in the hippocampus of the mouse brain. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. The embodiments are only used to explain the present invention and are not used to limit the protection scope of the present invention.

[0028] (I) Preparation of Chinese medicine composition 1. The proposal of Shenqi Funeng Xingnao Recipe Based on the characteristics and advantages of traditional Chinese medicine, and by optimizing the ideas and principles of Chinese medicine anti-fatigue prescriptions, a new Chinese medicine composition with the main effects of "strengthening the spleen and replenishing Qi, soothing the liver and regulating Qi, nourishing the liver and kidneys, and invigorating the mind" was proposed. It is called "Shenqi Funeng Awakening Brain Recipe". This prescription is intended to be developed into a medicine or health product that can be taken before and after flight missions to relieve pilots' fatigue and enhance their anti-fatigue ability.

[0029] The specific formula of the Shenqi Fu Neng Wangnao Refreshing Recipe is as follows: This recipe uses American ginseng and roasted astragalus to strengthen the spleen and replenish qi, combined with Ligustrum lucidum and Ecliptae to nourish the liver and kidneys. Lycium barbarum is sweet, mild and moist in nature, enters the liver and kidney meridians, replenishes the kidney and benefits the essence, nourishes the liver and improves eyesight; Chuanxiong and peppermint have the effects of soothing the liver, activating blood circulation and promoting qi; Polygala tenuifolia and Acorus calamus are used together to open the heart orifices, dredge the heart meridians, connect the heart and kidneys, benefit the kidneys, strengthen the brain and intelligence, and enhance the power of opening and closing the orifices and calming the mind.

[0030] 2. Example of preparation process of Chinese medicine composition 1) According to the Shenqi Funeng Xingnao Recipe, weigh the following Chinese medicinal materials: 30 parts by weight of roasted astragalus, 20 parts by weight of wolfberry, 20 parts by weight of Eclipta prostrata, 15 parts by weight of Ligustrum lucidum, 15 parts by weight of Polygala tenuifolia, 12 parts by weight of Chuanxiong, 10 parts by weight of American ginseng, 10 parts by weight of Acorus calamus, and 6 parts by weight of mint; 2) adding purified water equivalent to 10 times the total mass of each Chinese medicinal material in step 1, soaking for 2 hours, heating and boiling for 60 minutes, cooling, and filtering to obtain extract a and residue a; 3) adding purified water equivalent to 10 times the mass of the residue a to the residue a obtained in step 2, heating and boiling for 60 min, cooling, and filtering to obtain an extract b and a residue b; 4) adding purified water equivalent to 10 times the mass of the residue b to the residue b obtained in step 3, heating and boiling for 60 min, cooling, and filtering to obtain an extract c and a residue c; 5) The extracts a, b and c are combined, concentrated and spray-dried to obtain a dry paste powder which is an anti-fatigue Chinese medicine composition (abbreviated as SQFNXNP) prepared according to the various medicinal materials and their formula proportions in the above-mentioned Shenqi Funeng Xingnao Recipe.

[0031] (II) Study on the anti-fatigue effect of Chinese herbal medicine combination (pharmacology and pharmacodynamics experiments) 1. Experimental drugs and materials Experimental drugs: The Chinese medicine composition prepared in (I) (specifically SQFNXNP) was added to purified water to dissolve and vortex to prepare low- and high-dose (0.9 g / kg body weight, 6 g / kg body weight) Chinese medicine composition solutions (denoted as SQFNXNP-L and SQFNXNP-H). After preparation, the solutions were stored at 4°C and preheated at 37°C before each gavage. Experimental reagents: Liver / muscle glycogen assay kit (colorimetric method), L-lactic acid (L-LD) test kit (serum, tissue, etc.; colorimetric method), urea nitrogen (BUN) test kit (urease method), lactate dehydrogenase (LDH) kit (colorimetric method), creatine kinase (CK) assay kit (colorimetric method), total superoxide dismutase (T-SOD) assay kit (WST-1 method), malondialdehyde (MDA) assay kit (TBA method), glutathione peroxidase (GSH-PX) assay kit (colorimetric method), catalase (CAT) assay kit (visible light method, ammonium molybdate method) were all purchased from Nanjing Jiancheng Biotechnology Research Institute.

[0032] 2. Experimental animals and groups Thirty-two SPF Kunming male mice, 8-9 weeks old and weighing 18-22 g, were housed in the animal room of the Pharmacy Department of the First Affiliated Hospital of Air Force Medical University in a barrier environment with a room temperature of (22±1)°C, a relative humidity of 30%-60%, and a 12 h light / 12 h dark cycle. The animals were allowed to eat and drink freely.

[0033] 2.1 Acute fatigue experiment The experimental mice were divided into a normal control group (Control) and a Chinese medicine combination group (SQFNXNP-L, 0.9 g / kg body weight; SQFNXNP-H, 6 g / kg body weight). The mice were mainly subjected to an exhaustive swimming test to simulate acute exercise fatigue and verify the preventive intervention effect of the Chinese medicine combination on fatigue.

[0034] See also Figure 1 A and Figure 1 B, The experimental mice were subjected to 7 days (7 d) of adaptive feeding (Regular diet Acclimation), during which they first needed to undergo 3 days (i.e., the last 3 days of adaptive feeding) of adaptive swimming training, once a day, each time for about 10 min. Then, the two Chinese medicine composition groups were given different doses of Chinese medicine composition by gavage every day. After continuous oral administration (Oral administration) for 7 days, the mice were subjected to the exhaustion swimming time test (Exhausted swimming test) by swimming with a weight, that is, swimming with a lead wire of 5% of their own weight at the tail heel in a glass cylinder with a water depth of 30 cm, a water temperature of (30±2)℃, and a diameter×height of 15 cm×40 cm until exhaustion. The exhaustion judgment standard was: the mice could not float to the surface of the water for 5 consecutive seconds to breathe. The swimming time of mice in each group before exhaustion (i.e., exhaustion swimming time) was recorded. After the mice were exhausted, they were quickly fished out of the water, dried with an absorbent towel, and placed in a cage for normal feeding after the mice recovered their body temperature. They can continue to be used in subsequent experiments.

[0035] 2.2 Chronic fatigue experiment The experimental animals were divided into a normal control group (Control), a model group (Model), and a Chinese medicine composition group (SQFNXNP-L, 0.9 g / kg body weight; SQFNXNP-H, 6 g / kg body weight). A chronic fatigue mouse model was established by sleep deprivation combined with repeated forced swimming, including 24 h of sleep deprivation in a humid environment and 10 minutes of forced swimming per day. The modeling lasted for a total of 2 weeks. Finally, the effect of the Chinese medicine composition in anti-fatigue was verified by comparison with the established chronic fatigue animal model.

[0036] See also Figure 1 A and Figure 1B. Prepare a 30cm×20cm×15cm plastic box for raising animals. There is 2cm of water in the plastic box, and feed and water are placed on the lid of the box. At 11:00 on the same day, put the mice into the plastic box so that the water just covers the limbs of the mice. The mice can eat and drink freely, but they cannot sleep while soaking in water. The mice are taken out at 11:00 the next morning, and sleep deprivation is 24 hours in total. After that, forced swimming is used to achieve compound stress every day, that is, the mice are placed in a glass tank with a diameter of 18 cm, a height of 50 cm, and a water depth of 30 cm to swim. The water temperature is controlled at 23±2 ℃, and the forced swimming time is controlled every day. During the modeling period, the normal control group is fed normally and no stimulation is given. The model group is fed normally and receives sleep deprivation combined with repeated forced swimming stimulation. The two Chinese medicine composition groups are gavaged with different doses of Chinese medicine composition every day and receive sleep deprivation combined with repeated forced swimming stimulation. Since the extension of exercise time is the most powerful evidence for the improvement of anti-fatigue ability, after the model was established, behavioral tests were performed on mice in each group, including exhaustive swimming time test (loaded with 5% of their own weight), open field test (OFT), tail suspension test (TST), and forced swimming test (FST). FST and TST are used to quantitatively investigate the anti-fatigue effect and efficacy of drugs by examining the swimming time and struggling time of animals (such as fatigued mice) within 4 minutes. After 2 hours of behavioral testing, mice were anesthetized and the following tissue samples were collected: whole blood, gastrocnemius muscle, and brain tissue; whole blood samples were naturally coagulated at room temperature for 1 hour, centrifuged at 3000 r / min for 15 minutes, and the supernatant was collected and frozen at -80℃.

[0037] 3. Evaluation of anti-fatigue effect (1) Biochemical indicators: liver glycogen, muscle glycogen; lactate (LD), lactate dehydrogenase (LDH), creatine kinase (CK), blood urea nitrogen (BUN); superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), catalase (CAT); Creatine kinase (CK) is mainly present in the cytoplasm and mitochondria. It is an important kinase involved in intracellular energy conversion, ATP generation and muscle contraction. In addition, the most direct cause of fatigue is the consumption of glycogen in the body, and energy can be generated by the decomposition of glycogen. Therefore, the contents of creatine kinase, liver glycogen and muscle glycogen were measured. Due to excessive fatigue, glycolysis begins in the body to facilitate the continued supply of energy, and lactic acid (LD), as the final product of glycolysis, accumulates in the body in large quantities, lowering the pH value of muscles and blood, causing local muscle pain and fatigue, and in severe cases, damaging muscles. Lactate dehydrogenase (LDH) mainly catalyzes the mutual conversion between pyruvate and lactate. The increase in LDH concentration caused by high-intensity exercise may cause muscle damage. Therefore, LDH is also considered to be a specific marker of physical fatigue. In addition, with the increase of exercise time and intensity, carbohydrate metabolism cannot meet energy needs, protein and amino acid catabolism increases, and blood urea nitrogen (BUN) accumulates in the body as the main metabolite. Therefore, by measuring the levels of LD, LDH and BUN, the changes in the accumulation of metabolites in the body are explored. Reactive oxygen damage has been shown to be associated with fatigue-related diseases. Oxygen free radicals cause lipid peroxidation in biological membranes, and the final product is malondialdehyde (MDA). Due to lipid peroxidation in the cell membrane, the biological membrane structure is damaged, thereby changing the permeability of the membrane and impairing its function. In addition, MDA polymers react with macromolecules such as proteins and deoxyribonucleic acid to cause structural mutations, resulting in damaged cell function and cytotoxicity. Glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and catalase (CAT) have the ability to remove reactive oxygen species (ROS), such as superoxide anion free radicals (O 2- ), hydroxyl radical (OH - ) and hydrogen peroxide (H 2 O 2 )'s physiological activity. In addition, the level of SOD in the body is a direct indicator of the body's aging and death. It can resist and block the damage to cells caused by oxygen free radicals and repair damaged cells in time. Therefore, by measuring the levels of SOD, MDA, GSH-PX and CAT, the degree of oxidative damage in the body can be evaluated.

[0038] (2) Pathological morphology: H&E staining of gastrocnemius muscle, Nissl staining of hippocampus; TEM observation of gastrocnemius muscle, TEM observation of hippocampal mitochondria.

[0039] 4. Anti-fatigue effect analysis (1) Anti-fatigue Chinese medicine composition enhances the motor ability of mice According to the exhaustive swimming time of mice before entering the acute fatigue period ( Figure 1C), it can be clearly observed that compared with the normal control group (Control), the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition significantly increased the exhaustive swimming time of the weight-bearing mice, indicating that the Chinese medicine composition can enhance the physical strength of mice. According to the exhaustive swimming time of mice in the chronic fatigue period ( Figure 1 D), it can be clearly observed that compared with the model group (Model), the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition significantly increased the exhaustive swimming time of the weight-bearing mice, indicating that the Chinese medicine composition can restore and enhance the physical strength of the fatigued mice, thereby achieving the effect of restoring the physical strength of the fatigued mice. The results of OFT showed that the immobility time of the mice in the model group was prolonged ( Figure 1 E) Total distance moved ( Figure 1 F) reduction, Figure 1 The red area of ​​the model group mice in G increased, which also verified that the model mice stayed in a certain area for a longer time; compared with the model group mice, the corresponding groups (SQFNXNP-L and SQFNXNP-H) were given the Chinese medicine composition, which significantly increased the exercise time of fatigue mice and reduced the immobility time ( Figure 1 E, and increase the total distance moved ( Figure 1 F). The experimental results of FST and TST showed that compared with the model group mice, the Chinese medicine composition of the corresponding groups (SQFNXNP-L and SQFNXNP-H) significantly increased the swimming time of fatigue mice ( Figure 1 H) and tail suspension struggle time ( Figure 1 J), reduce swimming immobility time ( Figure 1 I) and tail suspension time ( Figure 1 K). The above mouse behavioral test results show that the Chinese medicine composition prepared based on the Shenqi Funeng Xingnao Recipe can significantly restore and increase the motor ability of fatigued mice and enhance the endurance of fatigued mice, thereby achieving the effect of anti-fatigue.

[0040] (2) Anti-fatigue Chinese medicine composition improves biochemical indicators in fatigue mice The experimental results of mice in the chronic fatigue period showed that compared with the normal control group (Control), the serum liver glycogen and muscle glycogen levels of the model group mice were significantly reduced, while compared with the model group mice, the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition significantly increased the serum liver glycogen and muscle glycogen levels of the mice ( Figure 2 B and Figure 2C), suggesting that the Chinese medicine composition may enhance the exercise endurance of fatigue mice by delaying the body's consumption of glycogen, activating energy metabolism, and exerting an anti-fatigue effect; compared with the model group mice, the serum CK content of the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition was significantly reduced ( Figure 2 A), which indirectly reflects that the Chinese medicine composition improves the fatigue tolerance of mice.

[0041] The experimental results of mice in the chronic fatigue period also showed that compared with the model group mice, the serum LD, LDH, and BUN levels of mice in the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition were significantly reduced ( Figure 2 D. Figure 2 E. Figure 2 F). This suggests that the Chinese medicine composition can effectively alleviate the accumulation of LD in fatigue mice, and on the other hand, it suggests that with the increase of exercise time and exercise intensity, the Chinese medicine composition can reduce the BUN level in fatigue mice, showing a significant anti-fatigue effect.

[0042] The above serum biochemical analysis results indicate that the Chinese medicine composition prepared based on the Shenqi Funeng Xingnao Refreshing Recipe can exert an anti-fatigue effect by inhibiting the excessive accumulation of metabolites, thereby achieving the effect of relieving fatigue.

[0043] The experimental results of mice in the chronic fatigue period also showed that compared with the normal control group, the MDA concentration in the serum of the model group mice increased sharply, while compared with the model group mice, the MDA concentration in the serum of the low-dose group (SQFNXNP-L) and high-dose group (SQFNXNP-H) of the Chinese medicine composition was significantly reduced ( Figure 2 H), at the same time, serum SOD, GSH-PX and CAT levels increased significantly ( Figure 2 G. Figure 2 I and Figure 2 J).

[0044] The above serum biochemical analysis results show that the Chinese medicine composition prepared based on the Shenqi Funeng Xingnao Refreshing Recipe can relieve body fatigue caused by oxidative damage by increasing the activity of antioxidant enzymes in the body, inhibiting lipid peroxidation, and reducing cell damage.

[0045] (3) Effect of the anti-fatigue Chinese medicine composition on the pathological morphology of the gastrocnemius muscle in fatigue mice like Figure 3As shown in A, the H&E staining of the gastrocnemius muscle of mice in the normal control group (Control) was uniform, the muscle fiber morphology and structure were normal, the arrangement was regular, and there was no obvious abnormality or inflammation in the interstitium, but the muscle fiber volume of the gastrocnemius muscle of the fatigue mice in the model group was reduced and the distance between them became wider; while for the mice given the Chinese medicine composition group (taking SQFNXNP-H as an example), the muscle fiber contraction caused by excessive fatigue or exercise was effectively alleviated.

[0046] like Figure 3 As shown in B, the number of mitochondria in the gastrocnemius cells of mice in the normal control group increased significantly, and mitochondrial autophagy and vacuolation were not obvious, but the number of mitochondria in the gastrocnemius cells of fatigue mice in the model group decreased significantly, and mitochondrial autophagy and vacuolation were obvious, indicating that excessive fatigue led to mitochondrial damage to intestinal muscle cells, insufficient energy, decreased motor (activity) ability, and fatigue state; while for the mice in the group given the Chinese medicine composition (taking SQFNXNP-H as an example), the number of mitochondria in their gastrocnemius cells increased effectively, the mitochondrial state and function were improved, the body's energy production and motor (activity) ability were enhanced, and the fatigue state was relieved.

[0047] Based on the experimental results of the above mice in the chronic fatigue period, it can be concluded that the Chinese medicine composition prepared based on the Shenqi Fu Neng Xingnao Refreshing Recipe can play a rejuvenating role on the fatigued body by significantly improving the pathological morphology of the gastrocnemius muscle tissue in the fatigued body.

[0048] (4) Effect of the anti-fatigue Chinese medicine composition on the pathological morphology of the hippocampus in fatigue mice like Figure 4 As shown in A, the number of neurons in the hippocampus of mice in the normal control group (Control) is large, the neurons are tightly arranged, the cell morphology is complete, the structure is clear, and they are rich in Nissl bodies. However, the neurons in the hippocampus of the fatigue mice in the model group are loosely arranged, the number is significantly reduced, and the Nissl bodies are reduced or dissolved; while for the mice in the group given the Chinese medicine composition (taking SQFNXNP-H as an example), the number of Nissl bodies in their hippocampus increased significantly, indicating that the Chinese medicine composition can increase the number of Nissl bodies in the hippocampus of fatigue mice.

[0049] like Figure 4 As shown in B, the number of mitochondria in the brain hippocampal cells of mice in the normal control group increased significantly, and mitochondrial autophagy and vacuolization were not obvious, but the number of mitochondria in the brain hippocampal cells of mice in the fatigue model group decreased significantly, and mitochondrial autophagy and vacuolization were obvious, indicating that excessive fatigue led to damage to the hippocampal mitochondria in the brain, insufficient energy, decreased motor (activity) ability, and fatigue state; while for the mice in the group given the Chinese medicine composition (taking SQFNXNP-H as an example), the number of mitochondria in the brain hippocampal cells was effectively increased, the mitochondrial state and function were improved, the body's energy production and motor (activity) ability were enhanced, and the fatigue state was relieved.

[0050] Based on the experimental results of the above mice in the chronic fatigue period, it can be concluded that the Chinese medicine composition prepared based on the Shenqi Fu Neng Xinnao Formula can significantly improve the pathological morphology of the hippocampus in the brain of the fatigued body, and has the function of refreshing the brain for the fatigued body, thereby exerting an anti-fatigue effect.

[0051] Based on the above results, it can be seen that the anti-fatigue effect of Shenqi Funeng Xingnao Fang is: (1) enhancing exercise capacity and fatigue tolerance; (2) delaying the body's consumption of glycogen and inhibiting the excessive accumulation of metabolites; (3) enhancing the level of antioxidant enzymes in the body, inhibiting lipid peroxidation, and reducing cell damage; (4) relieving muscle fiber contraction caused by excessive fatigue or exercise, and increasing the number of Nissl bodies in the hippocampus of fatigued bodies; (5) increasing the number of mitochondria in the gastrocnemius and hippocampus of fatigued bodies and improving the pathological morphology of mitochondria. It can be concluded that Shenqi Funeng Xingnao Fang has an anti-fatigue effect of restoring energy and refreshing the brain.

[0052] At the same time, some of the medicinal materials in the Shenqi Fu Neng Wangnao Fang are Chinese medicines that are both medicine and food, which is beneficial to ensuring the safety, low cost, and low toxicity and side effects of the corresponding Chinese medicine composition.

[0053] (III) Examples of adding or subtracting the dosage of Chinese medicinal materials in the Shenqi Funeng Xingnao Formula (1) If the Qi deficiency is severe, the dosage of American ginseng can be increased as appropriate (for example, to 12-15g) to enhance the Qi-tonifying effect; (2) For patients with severe Qi deficiency, the dosage of roasted Astragalus membranaceus can be appropriately increased (for example, to 40-50 g) to enhance the effect of replenishing Qi and strengthening the exterior. (3) If liver and kidney deficiency is more serious, such as backache and decreased vision, the dosage of wolfberry can be increased (for example, to 25-30g) to enhance the effect of nourishing the liver and kidney; (4) If liver and kidney yin deficiency is more obvious, the dosage of Ligustrum lucidum (e.g., to 15-20 g) and / or the dosage of Eclipta prostrata (e.g., to 20-25 g) can be increased to enhance the effects of tonifying the liver and kidney and nourishing yin. (5) If the symptoms of blood stasis are obvious, such as a dark purple tongue and a sluggish pulse, the dosage of Chuanxiong can be increased (for example, to 15-18 g) to enhance the blood-activating and blood-stasis-removing effects; (6) If there are obvious wind-heat symptoms, such as sore throat and headache, the dosage of mint can be slightly increased (for example, to 8g); (7) If restlessness and insomnia are more severe, the dosage of Polygala tenuifolia can be increased (for example, to 20 g) to enhance the calming effect; (8) If the phlegm and dampness are severe, such as a heavy head and thick and greasy tongue coating, the dosage of Acorus calamus can be appropriately increased (for example, to 12-15g) to enhance the effect of removing dampness and invigorating the spleen.

Claims

1. A Chinese medicine composition with anti-fatigue effect, characterized in that: The Chinese medicine composition is prepared from the following Chinese medicine raw materials: 20-50 parts of roasted astragalus or astragalus, 10-30 parts of wolfberry, 10-30 parts of Eclipta prostrata, 5-20 parts of Ligustrum lucidum, 5-20 parts of Polygala tenuifolia, 5-20 parts of Chuanxiong, 5-15 parts of American ginseng, 5-15 parts of Acorus calamus and 4-15 parts of mint.

2. A Chinese medicine composition with anti-fatigue effect according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following Chinese medicine raw materials: 25-35 parts of roasted astragalus or astragalus, 15-25 parts of wolfberry, 15-25 parts of Eclipta prostrata, 12-18 parts of Ligustrum lucidum, 12-18 parts of Polygala tenuifolia, 10-15 parts of Chuanxiong, 8-12 parts of American ginseng, 8-12 parts of Acorus calamus, and 5-8 parts of mint.

3. A Chinese medicine composition with anti-fatigue effect according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following Chinese medicine raw materials: 30 parts of roasted astragalus or astragalus, 20 parts of wolfberry, 20 parts of Eclipta prostrata, 15 parts of Ligustrum lucidum, 15 parts of Polygala tenuifolia, 12 parts of Chuanxiong, 10 parts of American ginseng, 10 parts of Acorus calamus, and 6 parts of mint.

4. A method for preparing a Chinese medicine composition having an anti-fatigue effect as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: Each Chinese medicine raw material is weighed according to the amount of medicine and then extracted with water. The obtained extract is concentrated and spray-dried to obtain a dry paste powder, which is the Chinese medicine composition.

5. The preparation method according to claim 4, characterized in that: The extraction specifically comprises the following steps: heating and leaching with 5-20 times the amount of water for 2-5 times, filtering after each leaching for 1-3 hours, and combining the filtrates obtained from each time.

6. Use of the Chinese medicinal composition with anti-fatigue effect as claimed in any one of claims 1 to 3 in the preparation of a medicament for preventing and / or relieving fatigue.

7. The use according to claim 6, characterized in that: The drug relieves chronic fatigue.

8. The use according to claim 6, characterized in that: The drug has the effects of enhancing athletic ability and muscle endurance, restoring physical strength, improving concentration and memory, and enhancing immunity.

9. The use according to claim 6, characterized in that: The drug has the effects of delaying the body's consumption of glycogen and inhibiting excessive accumulation of metabolites, enhancing the level of antioxidant enzymes in the body, inhibiting lipid peroxidation and reducing cell damage, relieving muscle fiber contraction and increasing the number of Nissl bodies in the hippocampus, as well as increasing the number of mitochondria in the gastrocnemius and hippocampus and improving the pathological morphology of mitochondria.

10. Use of the Chinese medicinal composition with anti-fatigue effect as claimed in any one of claims 1 to 3 in the preparation of health products or functional foods for relieving fatigue, improving memory and enhancing immunity.

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