Process for enhancing production of indoleacetic acid organic fertilizer by microorganisms

Abstract

The invention provides a process for microbial enhanced production of an indoleacetic acid organic fertilizer, and the process comprises the following steps: A, pig manure, a bacillus subtilis liquid and hermetia illucens larvae are put into a treatment pond and uniformly stirred, the concentration of the bacillus subtilis liquid is not lower than 1 * 10 < 8 > / L, and the concentration of the hermetia illucens larvae is not lower than 1 * 10 < 8 > / L; the adding amount of the bacillus subtilis liquid is 10-30 liters per ton of pig manure, and the adding amount of the black soldier fly larvae is 100 thousand-600 thousand larvae per ton of pig manure; b, the environment temperature of the treatment pond is kept at 20-35 DEG C, and after treatment reaches first preset time, a mixture of soybean meal and bacillus subtilis liquid is put into the treatment pond; and C, after the treatment reaches a second preset time, separating the hermetia illucens larvae and insect sand, and treating the insect sand to obtain the organic fertilizer containing indoleacetic acid and nitrogen, phosphorus and potassium elements. According to the method, the organic fertilizer containing indoleacetic acid can be prepared while the pig manure is treated.

Description

Technical Field

[0001] The invention relates to the field of organic fertilizer production, and more specifically to a process for producing indoleacetic acid organic fertilizer by microbial enhancement. Background Art

[0002] Indole-3-acetic acid is the main active ingredient of natural plant auxin and an organic compound naturally produced in plants. It mainly regulates the plant's phototropism, geotropism, apical dominance, fruit development and other physiological processes by promoting cell elongation, division and differentiation. Indole-3-acetic acid plays an irreplaceable role in key links such as seed germination, stem elongation, and root geotropism. By regulating the distribution and transportation of nutrients in plants, indole-3-acetic acid ensures that plants can efficiently use resources and achieve optimal growth.

[0003] At present, indoleacetic acid is mainly synthesized by the following method: indole, formaldehyde and potassium cyanide react at a high temperature of 150°C and a high pressure of 0.9-1 MPa to generate 3-indoleacetonitrile, which is then hydrolyzed under the action of potassium hydroxide, or indole reacts with glycolic acid to obtain it. However, the above method of obtaining indoleacetic acid is complicated and easily causes adverse effects on the environment.

[0004] In addition, plant fertilizers need to be rich in nitrogen, phosphorus and potassium, while pig manure contains more nitrogen and phosphorus and less potassium. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a process for producing indoleacetic acid organic fertilizer by microbial enhancement in view of the problems that the process for preparing indoleacetic acid is complicated and affects the environment.

[0006] The technical solution of the present invention to solve the above technical problems is to provide a process for producing indoleacetic acid organic fertilizer by microbial enhancement, the process comprising the following steps:

[0007] A. Put pig manure, Bacillus subtilis liquid and black soldier fly larvae into the treatment pool and stir them evenly. The concentration of the Bacillus subtilis liquid is not less than 1×10 8 The dosage of the Bacillus subtilis solution is 10-30 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 100,000-600,000 larvae per ton of pig manure;

[0008] B. maintaining the ambient temperature of the treatment tank at 20-35° C., and adding a mixture of soybean meal and Bacillus subtilis liquid into the treatment tank after the treatment reaches a first preset time;

[0009] C. After the treatment reaches a second preset time, the black soldier fly larvae and the insect sand are separated, and the organic fertilizer containing indoleacetic acid is obtained by treating the insect sand.

[0010] As a further improvement of the present invention, the first preset time in step B is 5-10 days, and the mixture is composed of 1-3 kg of soybean meal and 10-30 liters of a concentration not less than 1×10 8 The mixture is prepared by mixing 1000 μg / L of Bacillus subtilis bacterial liquid and soaking the mixture at room temperature for 3-6 hours.

[0011] As a further improvement of the present invention, the first preset time in step B is 6-9 days, and the mixture is composed of 2-3 kg of soybean meal and 20-30 liters of a concentration of not less than 1×10 8 The mixture is prepared by mixing 1000 μg / L of Bacillus subtilis bacterial liquid and soaking the mixture at room temperature for 4-5 hours.

[0012] As a further improvement of the present invention, the organic fertilizer containing indoleacetic acid is obtained by processing insect sand, which includes: drying the separated insect sand in a shaded and ventilated place for 1-4 days, and recovering the insect sand when the moisture content of the insect sand drops to 20-40%, so as to obtain the organic fertilizer containing indoleacetic acid.

[0013] As a further improvement of the present invention, the second preset time in step C is the 14th to 22nd day.

[0014] As a further improvement of the present invention, in step A, the Bacillus subtilis liquid and the black soldier fly larvae are added after the moisture content of the pig manure in the treatment tank is adjusted to 60-85%, and the individual size of the black soldier fly larvae reaches 0.02-0.06g.

[0015] As a further improvement of the present invention, in step A, the Bacillus subtilis liquid and the black soldier fly larvae are added after the moisture content of the pig manure in the treatment tank is adjusted to 70-80%.

[0016] As a further improvement of the present invention, the individual size of the black soldier fly larvae added in step A reaches 0.03-0.05 g.

[0017] As a further improvement of the present invention, the dosage of the Bacillus subtilis liquid is 15-25 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 200,000-500,000 larvae per ton of pig manure.

[0018] As a further improvement of the present invention, in step C, the insect sand is separated on the 16th to 21st day of treatment and dried in a shaded and ventilated place for 2 to 4 days, and the organic fertilizer containing indoleacetic acid is recovered when the moisture content reaches 20 to 30%.

[0019] The present invention has the following beneficial effects: by using Bacillus subtilis and soybean meal together, the production and content of indoleacetic acid are promoted in the process of black soldier fly larvae converting pig manure into insect sand organic fertilizer, thereby completing the preparation of organic fertilizer containing indoleacetic acid while achieving pig manure treatment. At the same time, the potassium element in Bacillus subtilis can also make up for the problem of insufficient potassium element in pig manure and insect sand, ensuring that the pig manure and insect sand are rich in nitrogen, phosphorus and potassium elements, and laying a foundation for the production of insect sand organic fertilizer rich in indoleacetic acid and nitrogen, phosphorus and potassium elements. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The present invention provides a schematic flow diagram of a process for producing indoleacetic acid organic fertilizer by microbial enhancement. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] Black soldier fly larvae can convert pig manure into insect sand organic fertilizer, which can be used in plant cultivation and organic agriculture. The existing black soldier fly insect sand has an organic matter content of about 50-80%, a nitrogen content of about 2.2-4.8%, a phosphorus content of about 0.5-2.1%, and a potassium content of about 0.2-2.3%, all of which have reached or exceeded my country's national standard for organic fertilizers NY252-2012, that is, the organic matter content is greater than 50%, and the nitrogen, phosphorus and potassium content is greater than 5%. However, in addition to nitrogen, phosphorus and potassium, black soldier fly insect sand may also contain another organic matter that is beneficial to plant growth, namely indoleacetic acid. However, the original indoleacetic acid content in pig manure is very low, and after using black soldier fly larvae to treat pig manure, the indoleacetic acid content in the insect sand is still not obvious.

[0023] like Figure 1 FIG. 1 is a schematic diagram of a process for producing indoleacetic acid organic fertilizer by microbial enhancement provided by an embodiment of the present invention, and the process comprises the following steps:

[0024] Step S1: Put pig manure, Bacillus subtilis liquid and black soldier fly larvae into the treatment tank and stir them evenly. The concentration of Bacillus subtilis liquid is not less than 1×10 8 The dosage of Bacillus subtilis liquid is 10-30 liters per ton of pig manure, and the dosage of black soldier fly larvae is 100,000-600,000 larvae per ton of pig manure. To facilitate temperature control, the above-mentioned treatment pool can be an indoor (or greenhouse) treatment pool with natural light or artificial light.

[0025] Bacillus subtilis can promote the production of indoleacetic acid, that is, by adding Bacillus subtilis liquid, microorganisms capable of producing indoleacetic acid are formed in the treatment tank. To improve the preparation efficiency, in this step, pig manure can be first put into the treatment tank, and the moisture content of the pig manure can be adjusted to 60-85% by adding water, and then Bacillus subtilis liquid is added to the treatment tank and mixed with the pig manure, and finally black soldier fly larvae are added to the treatment tank. The individual size of the black soldier fly larvae added to the treatment tank reaches 0.02-0.06g.

[0026] To further ensure the survival rate of black soldier flies and Bacillus subtilis, in this step, the Bacillus subtilis liquid and the black soldier fly larvae are preferably added after the moisture content of the pig manure in the treatment tank is adjusted to 70-80%. In addition, the individual size of the black soldier fly larvae added in this step is preferably 0.03-0.05g, the dosage of the Bacillus subtilis liquid is preferably 15-25 liters per ton of pig manure, and the dosage of the black soldier fly larvae is preferably 200,000-500,000 larvae per ton of pig manure.

[0027] Step S2: maintaining the ambient temperature of the treatment tank at 20-35° C., and adding a mixture of soybean meal and Bacillus subtilis liquid into the treatment tank after the treatment reaches a first preset time.

[0028] This step is mainly used to add Bacillus subtilis to the treatment pool and add protein that produces indoleacetic acid. In addition, step S2 can add the mixture 5-10 days after the black soldier fly larvae are added in step S1. In particular, the mixture added in the step is composed of 1-3 kg of soybean meal and 10-30 liters of indoleacetic acid with a concentration of not less than 1×10 8 The mixture is mixed with 100 g / L of Bacillus subtilis liquid and then soaked at room temperature for 3-6 hours to promote protein decomposition and improve the activity of Bacillus subtilis. Preferably, on the 14th to 22nd day after the black soldier fly larvae are added in step S1, a mixture of soybean meal and Bacillus subtilis liquid is added to the treatment tank, and the mixture is composed of 2-3 kg of soybean meal and a concentration of not less than 1×10 8 The mixture is prepared by mixing 20-30 liters of Bacillus subtilis liquid with 100 ng / L of each bacteria and soaking the mixture at room temperature for 4-5 hours.

[0029] Furthermore, in this step, it is best to maintain the ambient temperature of the treatment pool at 25-32° C. to further improve the activity of the black soldier fly larvae and Bacillus subtilis.

[0030] Step S3: After the treatment reaches a second preset time, the black soldier fly larvae and the insect sand are separated, and the organic fertilizer containing indoleacetic acid is obtained by treating the insect sand.

[0031] Since the black soldier fly larvae not only generate indoleacetic acid during the catabolism of tryptophan in protein, but also consume indoleacetic acid, the above-mentioned second preset time can be specifically the 16th to 21st day of the treatment, that is, the 16th to 21st day after the black soldier fly larvae are added in step S1, and the materials in the treatment pool are separated manually or mechanically to obtain black soldier fly larvae and insect sand, so that the indoleacetic acid content in the separated insect sand reaches a higher level.

[0032] In this step, the insect sand can be processed to obtain the organic fertilizer containing indoleacetic acid in the following manner: the separated insect sand is air-dried in a shaded and ventilated place for 1-4 days, and the insect sand is recovered when the moisture content is reduced to 20-40%, so as to obtain the organic fertilizer containing indoleacetic acid. Preferably, in order to ensure the preservation of the indoleacetic acid component in the organic fertilizer, the insect sand can be air-dried in a shaded and ventilated place for 2-4 days, and the organic fertilizer containing indoleacetic acid is recovered when the moisture content reaches 20-30%.

[0033] In addition, the black soldier fly larvae separated and obtained in this step can be frozen or dried for preservation and used as animal feed.

[0034] The above-mentioned microorganism strengthens the process of producing indoleacetic acid organic fertilizer, by using Bacillus subtilis and soybean meal in combination, using the ability of Bacillus subtilis to decompose tryptophan into indoleacetic acid, promoting the production and content of indoleacetic acid in the process of black soldier fly larvae converting pig manure into insect sand organic fertilizer, thereby completing the preparation of organic fertilizer containing indoleacetic acid while realizing pig manure treatment, helping to improve the fertilizer efficiency quality of insect sand organic fertilizer, and helping the coordinated development of agricultural green breeding industry. In the insect sand obtained by the process of the present invention, the indoleacetic acid content is not less than 10mg / kg, which provides an effective method for the production of organic fertilizer containing indoleacetic acid. At the same time, the potassium element in Bacillus subtilis can also make up for the problem of insufficient potassium element in pig manure insect sand, laying the foundation for the production of insect sand organic fertilizer rich in indoleacetic acid and nitrogen, phosphorus and potassium elements.

[0035] The following are several embodiments of the process for producing indoleacetic acid organic fertilizer by microbial enhancement.

[0036] Example 1

[0037] Step S11: Put the whole pig manure into the treatment pool, adjust the moisture content of the pig manure to 60%, and add 30 liters of water with a concentration of not less than 1×10 8 Bacillus subtilis liquid is added at a rate of 0.01-0.04g per liter and evenly mixed with pig manure. Black soldier fly larvae are then added at a ratio of 600,000 larvae per ton of pig manure, and the individual size of the larvae reaches 0.02-0.04g.

[0038] Step S12: The room temperature of the treatment process is maintained at 20-30°C, and the black soldier fly larvae are allowed to feed on pig manure and grow. On the 7th day of the treatment (i.e., the 7th day after the addition of the black soldier fly larvae), 1 kg of soybean meal is taken and 30 liters of a concentration of not less than 1×10 8 100g / L of Bacillus subtilis liquid, soak at room temperature for 3 hours, then add it into the treatment tank and stir evenly;

[0039] Step S14: On the 20th day of the treatment (i.e., the 7th day after adding the black soldier fly larvae), the black soldier fly larvae and the insect sand are separated manually or mechanically, and the separated insect sand is dried in a shaded and ventilated place for 1 day. When the moisture content drops to 25-30%, the drying is stopped, the insect sand is recovered, and the organic fertilizer containing indoleacetic acid is obtained.

[0040] After testing, the yield of the above-mentioned insect sand is 305.8kg / ton of pig manure, and the indoleacetic acid content in the insect sand reaches 17.3mg / kg (dry basis), the nitrogen content is 32.5mg / kg (dry basis), the phosphorus content is 34.3mg / kg (dry basis), and the potassium content is 37.9mg / kg (dry basis).

[0041] Example 2

[0042] Step S21: Put the whole pig manure into the treatment pool, adjust the moisture content of the pig manure to 75%, and add 20 liters of water with a concentration of not less than 1×10 8 Bacillus subtilis liquid is added at a rate of 100,000 larvae per ton of pig manure and mixed evenly with pig manure. Black soldier fly larvae are then added at a rate of 300,000 larvae per ton of pig manure, and the individual size of the larvae reaches 0.03-0.06g.

[0043] Step S22: The room temperature of the treatment process is maintained at 25-35°C, and the black soldier fly larvae are allowed to feed on pig manure and grow. On the fifth day of the treatment (i.e., the fifth day after the addition of the black soldier fly larvae), 2 kg of soybean meal is taken and 20 liters of a concentration of not less than 1×10 8 The mixture was added with Bacillus subtilis solution of 100 g / L and soaked at room temperature for 4 hours, then added into the black soldier fly treatment pool and stirred evenly.

[0044] Step S23: On the 14th day of the treatment (i.e., the 14th day after the black soldier fly larvae are added), the black soldier fly larvae and the insect sand are separated manually or mechanically, and the separated insect sand is air-dried in a shaded and ventilated place for 4 days. When the moisture content drops to 30-40%, the drying is stopped, the insect sand is recovered, and the organic fertilizer containing indoleacetic acid is obtained.

[0045] After testing, the yield of the above-mentioned insect sand is 208.1kg / ton of pig manure, the indoleacetic acid content in the insect sand reaches 36.1mg / kg (dry basis), the nitrogen content is 35.1mg / kg (dry basis), the phosphorus content is 32.2mg / kg (dry basis), and the potassium content is 33.2mg / kg (dry basis).

[0046] Example 3

[0047] Step S31: Put the whole pig manure into the treatment pool, adjust the moisture content of the pig manure to 85%, and add 10 liters of water with a concentration of not less than 1×10 8 Bacillus subtilis liquid is added at a rate of 100,000 larvae per ton of pig manure, and the individual size of the larvae reaches 0.04-0.06g.

[0048] Step S32: The room temperature of the treatment process is maintained at 27-35°C, and the black soldier fly larvae are allowed to feed on pig manure and grow. On the 10th day of the treatment (i.e., the 10th day after the addition of the black soldier fly larvae), 3 kg of soybean meal is taken and 30 liters of a concentration of not less than 1×10 8 The mixture was added with Bacillus subtilis solution of 100 g / L and soaked at room temperature for 6 hours, then added into the black soldier fly treatment pool and stirred evenly.

[0049] Step S34: On the 22nd day of the treatment (i.e., the 22nd day after the black soldier fly larvae are added), the black soldier fly larvae and the insect sand are separated manually or mechanically, and the separated insect sand is air-dried in a shaded and ventilated place for 4 days. When the moisture content drops to 35-40%, the drying is stopped, the insect sand is recovered, and the organic fertilizer containing indoleacetic acid is obtained.

[0050] After testing, the yield of the above-mentioned insect sand is 121.7kg / ton of pig manure, the indoleacetic acid content in the insect sand reaches 73.7mg / kg (dry basis), the nitrogen content is 37.8mg / kg (dry basis), the phosphorus content is 30.6mg / kg (dry basis), and the potassium content is 35.5mg / kg (dry basis).

[0051] Under the same conditions, without adding Bacillus subtilis culture solution and soybean meal, the content of indoleacetic acid in the obtained insect sand was almost undetectable.

[0052] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A process for producing indoleacetic acid organic fertilizer by microbial enhancement, characterized in that: The process comprises the following steps: A. Put pig manure, Bacillus subtilis liquid and black soldier fly larvae into the treatment pool and stir them evenly. The concentration of the Bacillus subtilis liquid is not less than 1×10 8 The dosage of the Bacillus subtilis solution is 10-30 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 100,000-600,000 larvae per ton of pig manure; B. maintaining the ambient temperature of the treatment tank at 20-35° C., and adding a mixture of soybean meal and Bacillus subtilis liquid into the treatment tank after the treatment reaches a first preset time; C. After the treatment reaches a second preset time, the black soldier fly larvae and the insect sand are separated, and the organic fertilizer containing indoleacetic acid is obtained by treating the insect sand.

2. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: The first preset time in step B is 5-10 days, and the mixture is composed of 1-3 kg of soybean meal and 10-30 liters of a concentration not less than 1×10 8 The mixture is prepared by mixing 1000 μg / L of Bacillus subtilis bacterial liquid and soaking the mixture at room temperature for 3-6 hours.

3. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: The first preset time in step B is 6-9 days, and the mixture is composed of 2-3 kg of soybean meal and 20-30 liters of a concentration not less than 1×10 8 The mixture is prepared by mixing 1000 μg / L of Bacillus subtilis bacterial liquid and soaking the mixture at room temperature for 4-5 hours.

4. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: The method of obtaining the organic fertilizer containing indoleacetic acid by treating the insect sand comprises: drying the separated insect sand in a shaded and ventilated place for 1-4 days, and recovering the insect sand when the moisture content is reduced to 20-40%, so as to obtain the organic fertilizer containing indoleacetic acid.

5. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: The second preset time in step C is day 14-22.

6. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: In the step A, the Bacillus subtilis liquid and the black soldier fly larvae are added to the pig manure in the treatment pool after the moisture content is adjusted to 60-85%, and the individual size of the black soldier fly larvae reaches 0.02-0.06g.

7. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 6, characterized in that: In the step A, the Bacillus subtilis liquid and the black soldier fly larvae are added after the moisture content of the pig manure in the treatment pool is adjusted to 70-80%.

8. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 6, characterized in that: The individual size of the black soldier fly larvae added in step A reaches 0.03-0.05 g.

9. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 6, characterized in that: The dosage of the Bacillus subtilis liquid is 15-25 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 200,000-500,000 larvae per ton of pig manure.

10. The process for producing indoleacetic acid organic fertilizer by microbial enhancement according to claim 1, characterized in that: In the step C, the insect sand is separated on the 16th to 21st day of the treatment and air-dried in a shaded and ventilated place for 2 to 4 days. When the moisture content reaches 20 to 30%, the organic fertilizer containing indoleacetic acid and nitrogen, phosphorus and potassium elements is recovered.

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