Stem cell culture medium filtering device

Abstract

The invention relates to the technical field of culture medium filtering, in particular to a stem cell culture medium filtering device which comprises a support, a mounting shell is arranged on the support, a screen drum, a driving unit, a feeding hopper, a discharging pipe and a high-pressure air duct are arranged on the mounting shell, the screen drum is horizontally arranged and located in the mounting shell, and the driving unit is used for driving the screen drum to rotate; the feeding hopper communicates with the interior of the screen drum, the discharging pipe communicates with the bottom of the mounting shell, the high-pressure air duct communicates with the top of the mounting shell, a high-pressure air pump communicates with the high-pressure air duct, and the high-pressure air pump can spray high-pressure air to the outer top wall of the screen drum through the high-pressure air duct; an impeller unit and a buoyancy unit are arranged in the screen drum, the impeller unit is rotationally connected to the screen drum and can be blown to rotate by high-pressure gas penetrating through screen holes of the screen drum, and the impeller unit abuts against the inner top wall of the screen drum in the rotating process and intermittently presses down the buoyancy unit. The filtering efficiency of the cell culture medium can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of culture medium filtration, and in particular to a stem cell culture medium filtration device. Background Art

[0002] Cell growth requires a nutritional environment, and the nutrient matrix used to maintain cell growth is called a culture medium. Cell culture medium is not only the basic substance that provides cell nutrition and promotes cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. In addition to beneficial components such as cytokines, cell culture medium also contains harmful substances such as waste products of cell growth metabolism, dead cells, and cell fragments, which need to be filtered before use.

[0003] Through searching, Chinese patent announcement number CN219792956U discloses a stem cell culture medium filter device, including a filter device body, a pair of first slots are provided on the two side walls of the filter device body, a connection block is provided in the pair of first slots, a spring is installed between the connection block and the first slot, a slide rail is provided in the connection block, a slider is slidably connected in the slide rail, a cell screen is installed on the slider, a pair of through holes are provided at the center of the two side walls of the filter device body, and a pull rope is passed through the pair of through holes. The utility model installs pull ropes on both sides of the cell screen. When a film is generated on the surface of the cell screen, the pull rope is pulled by the handle to drive the cell screen to move. When the pull rope is released, the spring will pull the cell screen back, and the film on the surface of the cell screen can be reduced under the action of inertia; when the surface of the cell screen is blocked by harmful substances, the cell screen can be slid out by opening the front cover plate to facilitate its cleaning.

[0004] With respect to the above-mentioned related technologies, the inventors found the following defects: the moving distance and vibration amplitude of the cell screen are limited, resulting in that the film on the surface of the cell screen cannot be effectively destroyed; in addition, the destroyed film is still scattered on the surface of the cell screen, so that the sieve holes of the cell screen are blocked, resulting in poor filtering effect of the cell screen; moreover, when a large amount of impurities accumulate on the surface of the cell screen, workers need to remove the cell screen for cleaning, resulting in the forced interruption of the filtration work, affecting the filtration efficiency of the cell culture medium, and therefore needs to be improved. Summary of the invention

[0005] In order to improve the filtration efficiency of cell culture medium, the present application provides a stem cell culture medium filtration device.

[0006] The present application provides a stem cell culture medium filtration device, which adopts the following technical solution: a stem cell culture medium filtration device, comprising a bracket, a mounting shell is provided on the bracket, a sieve drum, a driving unit, a feed hopper, a discharge pipe and a high-pressure air duct are provided on the mounting shell, the sieve drum is horizontally arranged and located inside the mounting shell, the driving unit is used to drive the sieve drum to rotate, the feed hopper is connected to the inside of the sieve drum, the discharge pipe is connected to the bottom of the mounting shell, the high-pressure air duct is connected to the top of the mounting shell, and the high-pressure air duct is connected to a high-pressure air pump, which can spray high-pressure gas to the outer top wall of the sieve drum through the high-pressure air duct; An impeller unit and a buoyancy unit are arranged in the screen drum. The impeller unit is rotatably connected to the screen drum and can be blown and rotated by high-pressure gas passing through the screen holes of the screen drum. During the rotation, the impeller unit will abut against the inner top wall of the screen drum and intermittently press down the buoyancy unit.

[0007] Optionally, the inner wall of the mounting shell and the outer wall of the sieve cylinder together form two high-pressure air cavities, which are respectively arranged on the left and right sides of the sieve cylinder. The upper ends of the two high-pressure air cavities are connected to the lower end of the high-pressure air duct, and the lower ends of the two high-pressure air cavities are inclined toward the lowest point of the sieve cylinder.

[0008] Optionally, the impeller unit includes a rotating shaft coaxially arranged with the screen drum, and a plurality of scrapers are provided on the rotating shaft which can be blown and rotated by high-pressure gas passing through the screen holes of the screen drum. During rotation, the scrapers will abut against the inner top wall of the screen drum and intermittently press down the buoyancy unit.

[0009] Optionally, the buoyancy unit includes a vertically arranged elastic telescopic rod, the upper end of the elastic telescopic rod is connected to a pressure plate, and the lower end of the elastic telescopic rod is connected to a floating plate; the scraper will press the pressure plate downward during rotation, and when one of the scrapers rotates away from the pressure plate, the next scraper will be located obliquely above the pressure plate and spaced apart from the pressure plate. At this time, the elastic telescopic rod will tend to return to its natural state and cause the pressure plate to rise and contact the scraper.

[0010] Optionally, two of the elastic telescopic rods are provided between the pressing plate and the floating plate, and limiting rings are slidably sleeved on the two elastic telescopic rods, and the limiting rings are installed on the inner wall of the installation shell.

[0011] Optionally, a cleaning plate is provided on the floating plate, and when the floating plate is not subjected to the buoyancy of the liquid, the cleaning plate will abut against the inner bottom wall of the screen cylinder.

[0012] Optionally, the inner bottom wall of the mounting shell and the outer bottom wall of the screen cylinder together form a discharge chamber, the lower ends of the two high-pressure air chambers are connected to the discharge chamber, the height of the bottom chamber wall of the discharge chamber gradually decreases from its two ends to its center, and the discharge pipe is connected to the lowest point of the discharge chamber.

[0013] Optionally, the screen drum is connected to the mounting shell by rotating around its own axis, and the driving unit includes a motor mounted on the mounting shell, a driving gear is coaxially connected to the output shaft of the motor, a driven gear is coaxially connected to the screen drum, and the driven gear is meshed with the driving gear.

[0014] In summary, this application includes the following beneficial technical effects: 1. The present application uses a cylindrical sieve cylinder to make the area available for culture medium filtration larger, thereby reducing the occurrence of the sieve cylinder being blocked by impurities and improving the filtration efficiency of the cell culture medium; 2. The rotation of the sieve cylinder causes impurities to gather on the inner bottom wall of the sieve cylinder under the dual effects of its own gravity and the flow force of the culture medium, reducing the impurities attached to other areas of the inner wall of the sieve cylinder. The culture medium can filter impurities through the peripheral area of ​​the inner bottom wall of the sieve cylinder, thereby further improving the filtration efficiency of the cell culture medium; 3. The high-pressure air pump can spray high-pressure gas to the outer top wall of the sieve cylinder through the high-pressure air duct. Part of the high-pressure gas will directly pass through the sieve holes of the sieve cylinder and enter the sieve cylinder, thereby clearing the sieve holes of the sieve cylinder and destroying the film; another part of the high-pressure gas will enter the two high-pressure air chambers along the outer top wall of the sieve cylinder and form a wind knife, which will scrape off the cells attached to the outer side wall of the sieve cylinder, so that these cells are blown down obliquely into the discharge chamber, so that these cells can be discharged through the discharge pipe along with the culture medium, thereby avoiding waste of resources; 4. When the high-pressure gas flows at high speed in the high-pressure air chamber, negative pressure is formed in the area inside the sieve cylinder close to the high-pressure air chamber. The culture medium in this area can pass through the sieve holes of the sieve cylinder into the high-pressure air chamber. The wind knife will drive this part of the culture medium into the discharge chamber, thereby improving the filtration efficiency of the cell culture medium. 5. When the air knife forces the gas and culture medium to pass through the sieve holes of the sieve cylinder, the film on the inner wall of the sieve cylinder will be destroyed to ensure the smooth flow of the sieve holes of the sieve cylinder, and the impurities around the sieve holes of the sieve cylinder will gather at the sieve holes of the sieve cylinder, so that the high-pressure gas in the high-pressure air duct can blow these impurities downward through the top sieve holes of the sieve cylinder; 6. The high-pressure gas entering the screen drum will be sprayed onto each scraper in turn. The scraper will rotate to scrape off the impurities and film attached to the top wall of the screen drum, minimizing the blockage of the screen drum; 7. When the scraper is blown and rotated by the high-pressure gas, each scraper will rotate in turn to press down the pressing plate, and the pressing plate will force the floating plate to press down the culture medium through the elastic telescopic rod, so that the culture medium passes through the sieve holes of the sieve cylinder faster, so as to improve the filtration efficiency of the cell culture medium; 8. The downward pressure of the floating plate and the collision of the pressing plate and the scraper will cause the culture medium to vibrate, preventing impurities from accumulating on the inner bottom wall of the sieve cylinder, thereby reducing the occurrence of sieve cylinder blockage and further improving the filtration efficiency of the cell culture medium; 9. When a small amount of culture medium exists inside the sieve cylinder or the culture medium is emptied, the gravity of the pressing plate and the elastic telescopic rod will prompt the floating plate to press the cleaning plate against the inner bottom wall of the sieve cylinder. At this time, the lower surface of the floating plate and the lower surface of the cleaning plate will form a collecting chamber together. The impurities and films on the inner wall of the sieve cylinder will be scraped off by the cleaning plate and accumulated in the collecting chamber, thereby realizing the rapid collection of impurities in the sieve cylinder for subsequent cleaning of the impurities. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the embodiment of the present application; Figure 2 It is a schematic cross-sectional view of the installation shell and the screen drum in the embodiment of the present application; Figure 3 It is a schematic structural diagram of a cross-section of the installation shell, the screen drum, the high-pressure air duct, the impeller unit and the buoyancy unit of the embodiment of the present application; Figure 4 It is a schematic diagram of the cross-sectional structure of the installation shell and the interior of the screen drum in the embodiment of the present application; Figure 5 It is a schematic diagram of the structure of the impeller unit and the buoyancy unit of the embodiment of the present application; Figure 6 It is a schematic diagram of the cross-sectional structure of the buoyancy unit of the embodiment of the present application.

[0016] Figure numerals: 1. bracket; 2. mounting shell; 21. cylinder; 22. sealing plate; 23. feed hopper; 24. discharge pipe; 25. high-pressure air chamber; 26. discharge chamber; 3. screen cylinder; 4. driving unit; 41. motor; 42. driving gear; 43. driven gear; 5. high-pressure air pump; 51. high-pressure air duct; 6. impeller unit; 61. rotating shaft; 62. scraper; 7. buoyancy unit; 71. elastic telescopic rod; 72. pressure plate; 73. floating plate; 74. limiting ring; 75. cleaning plate; 76. collecting chamber. DETAILED DESCRIPTION

[0017] The following is combined with Figure 1-6 This application is described in further detail.

[0018] The present application discloses a stem cell culture medium filtration device. Figure 1 and Figure 2As shown, a stem cell culture medium filtering device comprises a bracket 1, a mounting shell 2 and a sieve drum 3, wherein the mounting shell 2 comprises a cylinder 21 and a sealing plate 22 mounted on the bracket 1, wherein the cylinder 21 is horizontally arranged, and one end of the cylinder 21 is open; the sealing plate 22 is located at the open end of the cylinder 21, and the sealing plate 22 is disc-shaped and is provided with a gap with the open end of the cylinder 21; the sieve drum 3 is coaxially arranged with the cylinder 21, the sieve drum 3 is rotatably embedded in the cylinder 21 and is rotatably connected to the cylinder 21 around its own axis, and the sieve drum 3 is rotatably sleeved on the sealing plate 22 and is rotatably connected to the sealing plate 22 around its own axis, so that the sieve drum 3 can rotate.

[0019] The part of the screen drum 3 with the screen holes is located inside the cylinder 21, and the rest of the screen drum 3 is located outside the cylinder 21; the cylinder 21 is provided with a driving unit 4, which includes a motor 41 installed on the outer wall of the left end of the cylinder 21, and a driving gear 42 is coaxially connected to the output shaft of the motor 41, and a driven gear 43 is coaxially connected to the screen drum 3, and the driven gear 43 is meshed with the driving gear 42. The motor 41 can drive the driven gear 43 to rotate through the driving gear 42, and the driven gear 43 can drive the screen drum 3 to rotate.

[0020] The same feed hopper 23 is installed on the sealing plate 22 and the bracket 1. The upper end of the feed hopper 23 is flared, and the lower end of the feed hopper 23 is connected to the inside of the screen cylinder 3. The bottom of the cylinder 21 is connected to the discharge pipe 24. During the filtration process of the culture medium, the worker will pour the culture medium into the feed hopper 23, and the feed hopper 23 will guide the culture medium into the screen cylinder 3. The motor 41 will drive the screen cylinder 3 to rotate through the driving gear 42 and the driven gear 43. The impurities in the culture medium will accumulate on the inner bottom wall of the screen cylinder 3, and the culture medium with impurities removed will pass through the sieve holes of the screen cylinder 3 and be discharged through the discharge pipe 24 at the bottom of the cylinder 21.

[0021] It is worth noting that, compared with flat and curved sieves, the sieve cylinder 3 in the present application is cylindrical, and the sieve cylinder 3 can rotate during the filtration process, so that each area on the side of the sieve cylinder 3 can be used for filtering the culture medium, that is, the area that can be used for culture medium filtration in the present application is larger, thereby reducing the occurrence of clogging of the sieve cylinder 3 by impurities and improving the filtration efficiency of the cell culture medium.

[0022] In addition, during the rotation of the sieve cylinder 3, some impurities will tend to gather on the inner bottom wall of the sieve cylinder 3 under the dual effects of their own gravity and the flow force of the culture medium, reducing the impurities attached to other areas of the inner wall of the sieve cylinder 3. The culture medium can be filtered through the peripheral area of ​​the inner bottom wall of the sieve cylinder 3, thereby further improving the filtration efficiency of the cell culture medium.

[0023] like Figure 2 and Figure 3As shown, the top of the cylinder 21 is connected to a high-pressure air duct 51, and the top of the high-pressure air duct 51 is connected to a high-pressure air pump 5. The high-pressure air pump 5 can spray high-pressure gas to the outer top wall of the sieve cylinder 3 through the high-pressure air duct 51, and the high-pressure gas can pass downward through the sieve holes of the sieve cylinder 3, so that the impurities blocked in the sieve holes of the sieve cylinder 3 are pushed out to the inside of the sieve cylinder 3, thereby achieving the dredging of the sieve holes of the sieve cylinder 3; and the high-pressure airflow passing through the sieve holes of the sieve cylinder 3 will destroy the film on the inner wall of the sieve cylinder 3, reducing the blockage of the sieve cylinder 3, so that the culture medium in the sieve cylinder 3 can complete the filtration work faster, thereby improving the filtration efficiency of the cell culture medium.

[0024] The inner wall of the cylinder 21 and the outer wall of the sieve cylinder 3 together form two high-pressure air chambers 25. The two high-pressure air chambers 25 are both arranged in an arc shape and are respectively arranged on the left and right sides of the sieve cylinder 3. The upper ends of the two high-pressure air chambers 25 are connected to the lower end of the high-pressure air duct 51, and the lower ends of the two high-pressure air chambers 25 are inclined toward the lowest point of the sieve cylinder 3.

[0025] When the high-pressure gas in the high-pressure air duct 51 is sprayed onto the outer top wall of the sieve cylinder 3, part of the high-pressure gas will directly pass through the sieve holes of the sieve cylinder 3 and enter the sieve cylinder 3, thereby clearing the sieve holes of the sieve cylinder 3 and destroying the film; another part of the high-pressure gas will enter the two high-pressure air chambers 25 along the outer top wall of the sieve cylinder 3. The thickness of the high-pressure air chamber 25 is relatively small, so that the high-pressure gas in the high-pressure air chamber 25 can form a wind knife during the downward blowing process.

[0026] It is worth mentioning that when the culture medium with impurities removed passes downward through the bottom sieve holes of the sieve cylinder 3, the culture medium will gather to form droplets due to surface tension and its own gravity. At this time, some cells in the droplets will adhere to the outer wall of the sieve cylinder 3; and the wind knife in the high-pressure air chamber 25 will scrape off the cells attached to the outer wall of the sieve cylinder 3, so that these cells are blown downward at an angle to the inner bottom wall of the cylinder 21, so that these cells can be discharged along with the culture medium through the discharge pipe 24, avoiding waste of resources.

[0027] The inner bottom wall of the cylinder 21 and the outer bottom wall of the sieve cylinder 3 together form a discharge chamber 26. The thickness of the discharge chamber 26 is greater than the thickness of the high-pressure air chamber 25, thereby reserving sufficient space for the flow of the culture medium; the lower ends of the two high-pressure air chambers 25 are connected to the discharge chamber 26, so that the wind knife in the high-pressure air chamber 25 can blow the cells on the outer wall of the sieve cylinder 3 into the discharge chamber 26; the height of the bottom cavity wall of the discharge chamber 26 gradually decreases from its two ends to its center, so that the culture medium in the discharge chamber 26 will converge to the center of the bottom cavity wall of the discharge chamber 26; the discharge pipe 24 is connected to the lowest point of the discharge chamber 26, so as to facilitate the discharge of the gathered culture medium through the discharge pipe 24.

[0028] It is worth noting that when the high-pressure gas flows at a high speed in the high-pressure air chamber 25, the air in the sieve cylinder 3 will pass through the sieve holes of the sieve cylinder 3 and enter the high-pressure air chamber 25, so that a negative pressure is formed in the area inside the sieve cylinder 3 close to the high-pressure air chamber 25. Therefore, the culture medium located in this part of the area can pass through the sieve holes of the sieve cylinder 3 and enter the high-pressure air chamber 25, and the wind knife will drive this part of the culture medium to enter the discharge chamber 26, that is, the culture medium can directly pass through the sieve holes of the sieve cylinder 3 and enter the discharge chamber 26, and can also pass through the sieve holes of the sieve cylinder 3 and the high-pressure air chamber 25 in sequence and enter the discharge chamber 26. The combination of the two filtering paths improves the filtering efficiency of the cell culture medium; in addition, in the process of the gas and the culture medium passing through the sieve holes of the sieve cylinder 3, the film on the inner wall of the sieve cylinder 3 will be destroyed to ensure the smooth flow of the sieve holes of the sieve cylinder 3, and the impurities located around the sieve holes of the sieve cylinder 3 will gather at the sieve holes of the sieve cylinder 3, so that the high-pressure gas in the high-pressure air duct 51 can blow these impurities downward through the top sieve holes of the sieve cylinder 3.

[0029] like Figures 3 to 5 As shown, an impeller unit 6 and a buoyancy unit 7 are provided in the screen drum 3. The impeller unit 6 includes a rotating shaft 61 which is coaxially arranged with the screen drum 3. The rotating shaft 61 is located in the middle and upper part of the screen drum 3, and the rotating shaft 61 rotates around its own axis and is connected to the sealing plate 22 and the cylinder body 21. A plurality of scrapers 62 are circumferentially installed on the side wall of the rotating shaft 61.

[0030] During the filtering process of the culture medium, part of the high-pressure gas will pass through the sieve holes of the sieve cylinder 3 and enter the sieve cylinder 3. This part of the high-pressure gas will be sprayed onto one of the scrapers 62, causing the scraper 62 to flip downward. The scraper 62 will drive the rotating shaft 61 to rotate, and the rotating shaft 61 will drive the other scrapers 62 to rotate synchronously. The scraper 62 will come into contact with the inner top wall of the sieve cylinder 3 during the rotation, so that the impurities and films attached to the inner top wall of the sieve cylinder 3 are scraped off, thereby minimizing the blockage of the sieve cylinder 3. In addition, the scraped impurities and films will adhere to the side of the scraper 62 away from the rotating shaft 61, and this side of the scraper 62 will be sprayed with high-pressure gas during the rotation, so that the impurities and films on this side of the scraper 62 are blown off, so as to ensure the scraping effect of the scraper 62 on the inner wall of the sieve cylinder 3.

[0031] It is worth noting that the scraper 62 extends along the axial direction of the sieve cylinder 3, the front end of the scraper 62 abuts against the sealing plate 22, and the rear end of the scraper 62 abuts against the inner end wall of the cylinder 21, so that the scraper 62 can scrape off the impurities and films inside the sieve cylinder 3 more comprehensively; when the cleaned area on the inner wall of the sieve cylinder 3 rotates to the bottom of the sieve cylinder 3, the culture medium will be able to quickly pass through the sieve holes in this area into the discharge chamber 26, thereby improving the filtration efficiency of the cell culture medium.

[0032] In addition, the scraper 62 can be made of a plastic plate, which is relatively inexpensive, has a low density, and is light in weight, making it easy to be blown and rotated by the high-pressure gas entering the screen drum 3 .

[0033] like Figure 3 , Figure 5 and Figure 6 As shown, the buoyancy unit 7 includes two vertically arranged elastic telescopic rods 71, which are composed of an outer tube, an inner rod and a spring. One end of the inner rod is slidably embedded in the outer tube, and the end of the inner rod is connected to the outer tube through a spring; the upper ends of the two elastic telescopic rods 71 ​​are connected to the same pressing plate 72, and the lower ends of the two elastic telescopic rods 71 ​​are connected to the same floating plate 73.

[0034] During the filtering process of the culture medium, the floating plate 73 will float on the culture medium liquid surface inside the sieve cylinder 3, and at this time, the floating plate 73 will support the pressing plate 72 through the elastic telescopic rod 71; when the scraper 62 is blown and rotated by the high-pressure gas, each scraper 62 will rotate in turn to contact the pressing plate 72, and the pressing plate 72 will be pressed down by the scraper 62, so that the elastic telescopic rod 71 is compressed, and the elastic telescopic rod 71 will quickly press down the culture medium in the sieve cylinder 3 through the floating plate 73, and the culture medium will be accelerated to pass through the sieve holes of the sieve cylinder 3 after being subjected to the downward pressure, so as to improve the filtering efficiency of the cell culture medium; in addition, the downward pressure of the floating plate 73 will cause the culture medium to oscillate, and prevent impurities from being statically accumulated on the inner bottom wall of the sieve cylinder 3, thereby reducing the occurrence of blockage of the sieve cylinder 3 and further improving the filtering efficiency of the cell culture medium.

[0035] It is worth mentioning that the upper surface of the pressure plate 72 is arranged in an arc shape. When the scraper 62 is pressing the pressure plate 72 downward, the end of the scraper 62 away from the rotating shaft 61 will continue to be pressed against the upper surface of the pressure plate 72. At this time, the impurities and films on the scraper 62 that cannot be blown off by the high-pressure gas will fall off due to the friction between the scraper 62 and the pressure plate 72, so that the surface of the scraper 62 remains clean, thereby ensuring the cleaning effect of the scraper 62 on the inside of the sieve drum 3; in addition, the impurities and films that fall from the scraper 62 to the upper surface of the pressure plate 72 will also be scraped off by the scraper 62, thereby avoiding the residue of impurities and films on the pressure plate 72 as much as possible.

[0036] Because impurities and films detached from the scraper 62 and the pressure plate 72 may fall onto the upper surface of the floating plate 73, the upper surface of the floating plate 73 can be set at an angle. When the elastic telescopic rod 71 drives the floating plate 73 to press down the culture medium, the floating plate 73 will sink completely into the culture medium. The culture medium shaken by the floating plate 73 will carry away the impurities and films on the upper surface of the floating plate 73 during the flow, thereby avoiding the residue of impurities and films on the floating plate 73 as much as possible.

[0037] Limiting rings 74 are slidably sleeved on the two elastic telescopic rods 71, one of which is installed on the sealing plate 22, and the other is installed on the inner end wall of the cylinder 21. The two limiting rings 74 jointly limit the buoyancy unit 7 so that the buoyancy unit 7 can only be raised and lowered, ensuring that the scraper 62 can accurately press down the pressing plate 72 and completely clean the impurities and films on the pressing plate 72.

[0038] It is worth noting that when one of the scrapers 62 rotates and separates from the pressure plate 72, the next scraper 62 will be located obliquely above the pressure plate 72 and spaced apart from the pressure plate 72. At this time, the elastic telescopic rod 71 will tend to return to its natural state, and the floating plate 73 will float up due to the buoyancy of the culture medium; under the dual action of the rebound force of the elastic telescopic rod 71 and the buoyancy of the floating plate 73, the pressure plate 72 will rise rapidly and hit the next scraper 62, causing both the scraper 62 and the floating plate 73 to vibrate; the vibration of the scraper 62 itself will facilitate the separation of impurities and film from its side, and the scraper 62 can transmit the vibration to the sieve cylinder 3 when in contact with the sieve cylinder 3, so that the impurities blocked in the sieve holes of the sieve cylinder 3 are loosened so that the impurities can be separated from the sieve holes of the sieve cylinder 3; and the vibration of the floating plate 73 itself will be transmitted to the culture medium, aggravating the vibration of the culture medium, and further preventing impurities from being statically accumulated on the inner bottom wall of the sieve cylinder 3.

[0039] A cleaning plate 75 is installed on the floating plate 73. The cleaning plate 75 is inclined, and the cleaning plate 75 and the floating plate 73 together form an inverted V-shaped plate, thereby minimizing the residue of impurities and films on the floating plate 73 and the cleaning plate 75; when there is a large amount of culture medium in the sieve cylinder 3, the floating plate 73 will float on the liquid surface of the culture medium, and the cleaning plate 75 will be spaced apart from the inner bottom wall of the sieve cylinder 3; when there is a small amount of culture medium in the sieve cylinder 3 or the culture medium is emptied, the gravity of the pressing plate 72 and the elastic telescopic rod 71 will prompt the floating plate 73 to press the cleaning plate 75 against the inner bottom wall of the sieve cylinder 3. At this time, the lower surface of the floating plate 73 will form a collecting chamber 76 together with the lower surface of the cleaning plate 75. The impurities and films on the inner wall of the sieve cylinder 3 will be scraped off by the cleaning plate 75 and accumulated in the collecting chamber 76, thereby realizing the rapid collection of impurities in the sieve cylinder 3 for subsequent cleaning of impurities.

[0040] It is worth mentioning that cleaning ports can be opened at the ends of the sealing plate 22 and the cylinder 21, and an openable and closable door can be set at the cleaning port. Clean water can enter the collecting chamber 76 from one cleaning port. Under the flushing of clean water, impurities in the collecting chamber 76 can be discharged through another cleaning port, thereby realizing rapid cleaning of impurities in the screen cylinder 3.

[0041] The implementation principle of a stem cell culture medium filtration device in an embodiment of the present application is as follows: during the filtration process of the cell culture medium, a worker will pour the culture medium into the feed hopper 23, the feed hopper 23 will guide the culture medium into the sieve cylinder 3, the motor 41 will drive the sieve cylinder 3 to rotate through the driving gear 42 and the driven gear 43, the impurities in the culture medium will accumulate on the inner bottom wall of the sieve cylinder 3, the culture medium with impurities removed will pass through the sieve holes of the sieve cylinder 3 and enter the discharge chamber 26, and the culture medium in the discharge chamber 26 will be discharged through the discharge pipe 24.

[0042] At the same time, the high-pressure air pump 5 will spray high-pressure gas toward the outer top wall of the sieve cylinder 3 through the high-pressure air duct 51, and part of the high-pressure gas will directly pass through the sieve holes of the sieve cylinder 3 and enter the sieve cylinder 3, so as to clear the sieve holes of the sieve cylinder 3 and destroy the film. Then, this part of the high-pressure gas will be sprayed onto each scraper 62 in turn, and the scraper 62 will rotate to scrape off the impurities and film attached to the inner top wall of the sieve cylinder 3. The impurities and film on the scraper 62 will be blown off by this part of the high-pressure gas, and each scraper 62 will rotate to press down the pressing plate 72 in turn. The pressing plate 72 will prompt the floating plate 73 to quickly press down the culture medium in the sieve cylinder 3 through the elastic telescopic rod 71, so that the culture medium is accelerated to pass through the sieve holes of the sieve cylinder 3 and prompt the culture medium to vibrate, so as to avoid impurities from being statically accumulated on the inner bottom wall of the sieve cylinder 3. Another part of the high-pressure gas will enter the two high-pressure air chambers 25 along the outer top wall of the sieve cylinder 3 and form a wind knife. The wind knife in the high-pressure air chamber 25 will scrape off the cells attached to the outer wall of the sieve cylinder 3, so that this part of the cells is blown downward obliquely into the discharge chamber 26, and a negative pressure will be formed in the area inside the sieve cylinder 3 close to the high-pressure air chamber 25. The culture medium located in this part of the area can pass through the sieve holes of the sieve cylinder 3 into the high-pressure air chamber 25, and the wind knife will drive this part of the culture medium into the discharge chamber 26. At this time, the film on the inner wall of the sieve cylinder 3 will be destroyed by the flowing culture medium, and the impurities located around the sieve holes of the sieve cylinder 3 will gather at the sieve holes of the sieve cylinder 3, so that the high-pressure gas in the high-pressure air duct 51 will blow this part of the impurities downward through the top sieve holes of the sieve cylinder 3.

[0043] When one of the scrapers 62 rotates and separates from the pressure plate 72, the next scraper 62 will be located obliquely above the pressure plate 72 and spaced apart from the pressure plate 72. The resilience of the elastic telescopic rod 71 and the buoyancy of the floating plate 73 will cause the pressure plate 72 to rise rapidly and hit the next scraper 62, causing both the scraper 62 and the floating plate 73 to vibrate. The vibration of the scraper 62 itself will facilitate the separation of impurities and film from its side, and the scraper 62 can transmit the vibration to the sieve cylinder 3 when in contact with the sieve cylinder 3, so that the impurities blocked in the sieve holes of the sieve cylinder 3 are loosened so that the impurities can be separated from the sieve holes of the sieve cylinder 3. The vibration of the floating plate 73 itself will be transmitted to the culture medium, thereby intensifying the vibration of the culture medium and further preventing impurities from being statically accumulated on the inner bottom wall of the sieve cylinder 3.

[0044] When the culture medium inside the sieve cylinder 3 is about to be emptied, the gravity of the pressing plate 72 and the elastic telescopic rod 71 will prompt the floating plate 73 to press the cleaning plate 75 against the inner bottom wall of the sieve cylinder 3. At this time, the lower surface of the floating plate 73 will form a collecting chamber 76 together with the lower surface of the cleaning plate 75. The impurities and films on the inner wall of the sieve cylinder 3 will be scraped off by the cleaning plate 75 and accumulated in the collecting chamber 76, thereby realizing the rapid collection of impurities in the sieve cylinder 3 for subsequent cleaning of the impurities.

[0045] In summary, the present application improves the filtration efficiency of the cell culture medium in many aspects through a larger filtration area, reducing the attachment of impurities and films on the inner wall of the sieve cylinder 3, clearing the sieve holes of the sieve cylinder 3 and destroying the film by high-pressure gas, scraping and suction traction of the culture medium by the wind knife, scraping of impurities and films on the inner wall of the sieve cylinder 3 by the scraper 62, downward pressure and vibration of the culture medium by the floating plate 73, and automatic cleaning and collection of impurities in the sieve cylinder 3 by the cleaning plate 75.

[0046] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A stem cell culture medium filtration device, characterized in that: The invention comprises a support (1), a mounting shell (2) being provided on the support (1), a screen drum (3), a driving unit (4), a feed hopper (23), a discharge pipe (24) and a high-pressure air duct (51) being provided on the mounting shell (2), the screen drum (3) being arranged horizontally and being located inside the mounting shell (2), the driving unit (4) being used to drive the screen drum (3) to rotate, the feed hopper (23) being connected to the inside of the screen drum (3), the discharge pipe (24) being connected to the bottom of the mounting shell (2), the high-pressure air duct (51) being connected to the top of the mounting shell (2), and a high-pressure air pump (5) being connected to the high-pressure air duct (51), and the high-pressure air pump (5) being able to spray high-pressure gas onto the outer top wall of the screen drum (3) through the high-pressure air duct (51); An impeller unit (6) and a buoyancy unit (7) are provided in the screen drum (3). The impeller unit (6) is rotatably connected to the screen drum (3) and can be blown and rotated by high-pressure gas passing through the screen holes of the screen drum (3). During the rotation process, the impeller unit (6) will abut against the inner top wall of the screen drum (3) and intermittently press the buoyancy unit (7) downward.

2. A stem cell culture medium filtration device according to claim 1, characterized in that: The inner wall of the mounting shell (2) and the outer wall of the sieve drum (3) together enclose two high-pressure air chambers (25); the two high-pressure air chambers (25) are arranged on the left and right sides of the sieve drum (3); the upper ends of the two high-pressure air chambers (25) are connected to the lower end of the high-pressure air duct (51); and the lower ends of the two high-pressure air chambers (25) are inclined toward the lowest point of the sieve drum (3).

3. A stem cell culture medium filtration device according to claim 2, characterized in that: The impeller unit (6) comprises a rotating shaft (61) arranged coaxially with the sieve drum (3); the rotating shaft (61) is provided with a plurality of scrapers (62) which can be driven to rotate by high-pressure gas passing through the sieve holes of the sieve drum (3); during the rotation process, the scrapers (62) will abut against the inner top wall of the sieve drum (3) and intermittently press down the buoyancy unit (7).

4. A stem cell culture medium filtration device according to claim 3, characterized in that: The buoyancy unit (7) comprises a vertically arranged elastic telescopic rod (71), the upper end of the elastic telescopic rod (71) is connected to a pressure plate (72), and the lower end of the elastic telescopic rod (71) is connected to a floating plate (73); the scraper (62) presses down the pressure plate (72) during rotation, and when one of the scrapers (62) rotates and separates from the pressure plate (72), the next scraper (62) will be located obliquely above the pressure plate (72) and spaced apart from the pressure plate (72), and at this time the elastic telescopic rod (71) will tend to return to a natural state and cause the pressure plate (72) to rise and contact the scraper (62).

5. A stem cell culture medium filtration device according to claim 4, characterized in that: Two elastic telescopic rods (71) are provided between the pressing plate (72) and the floating plate (73), and a limiting ring (74) is slidably sleeved on each of the two elastic telescopic rods (71), and the limiting ring (74) is mounted on the inner wall of the mounting shell (2).

6. A stem cell culture medium filtration device according to claim 5, characterized in that: A cleaning plate (75) is provided on the floating plate (73). When the floating plate (73) is not subjected to the buoyancy of the liquid, the cleaning plate (75) will abut against the inner bottom wall of the screen cylinder (3).

7. The stem cell culture medium filtration device according to claim 2, characterized in that: The inner bottom wall of the mounting shell (2) and the outer bottom wall of the screen drum (3) together enclose a discharge chamber (26); the lower ends of the two high-pressure air chambers (25) are both connected to the discharge chamber (26); the height of the bottom chamber wall of the discharge chamber (26) gradually decreases from both ends to the center; the discharge pipe (24) is connected to the lowest point of the discharge chamber (26).

8. A stem cell culture medium filtration device according to any one of claims 1 to 7, characterized in that: The screen drum (3) is connected to the mounting shell (2) so as to rotate around its own axis. The driving unit (4) comprises a motor (41) mounted on the mounting shell (2). A driving gear (42) is coaxially connected to the output shaft of the motor (41). A driven gear (43) is coaxially connected to the screen drum (3). The driven gear (43) is meshed with the driving gear (42).

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