Independent rotation shake culture device

Abstract

The invention discloses an independent rotating and shaking culture device, and belongs to the technical field of biological culture equipment.The independent rotating and shaking culture device comprises a program control host and an environment adjusting system, the environment adjusting system comprises a plurality of cabins distributed in an array mode, rotating and shaking mechanisms are arranged in the cabins and comprise driving motors, and the driving motors are connected with rotating shafts; a motion conversion assembly is arranged on the rotating shaft and comprises a guide shaft, a sliding conversion frame and a guide bearing, the guide bearing is arranged in a guide groove in the circumferential side of the rotating shaft, the guide groove is in a wave shape, and the sliding conversion frame is connected with a non-rotating part of the crossed ball bearing; the rotating shaft and the rotating spline are connected with the rotating part of the crossed ball bearing, the rotating spline is connected with a tray, and the tray is connected with a culture dish. By the adoption of the independent rotation shaking culture device, independent culture of the multiple cabins is achieved, rotation and telescopic shaking are achieved through one motor, the equipment cost is reduced, and the nutrition exchange efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological culture equipment, and in particular to an independent rotating and shaking culture device. Background Art

[0002] The rotating biological culture device is a cell culture device that is widely used in the fields of bioengineering, pharmaceutical research and development, tissue engineering and biotechnology. In the field of biological cell culture, biological sample culture usually adopts static culture method (biological culture samples are statically suspended in nutrients or adsorbed on the inner wall of culture vessels for culture). When the biological sample cells develop into cell aggregates, the nutrient exchange between cells and culture fluid is insufficient and the culture environment does not meet the requirements of the original organism conditions, resulting in slow development or death of the biological sample cells. The rotating biological culture method uses the centrifugal force and fluid dynamics generated by rotation to optimize the cell growth environment, which can support the development of biological sample cells into cell aggregates.

[0003] The patent with announcement number CN217809496U discloses an oscillation and mixing device for biological cell culture, which relates to the field of biological cell technology, including a box body, an inner shell body is arranged inside the box body, the bottom of the inner shell body is connected to the insertion rod through two ring buckles, an annular disk is fixedly connected to the top of the inner shell body, a rotating disk is arranged in the middle of the annular disk, the bottom of the rotating disk is fixedly connected to the rotating motor, a fixing rod is fixedly connected between the inner walls of the annular disk groove, a transmission rod is rotatably connected to the outer wall of the fixing rod, and the other end of the transmission rod is rotatably connected to the eccentric block on the outer wall of the eccentric wheel, and the rotating shaft of the eccentric wheel is fixedly connected to the oscillation motor. By arranging the rotating motor and the oscillation motor, and using them in conjunction with the eccentric wheel and the transmission rod, the biological cell culture tube on the rotating disk can be made to oscillate up and down while rotating, thereby accelerating its oscillation and mixing speed.

[0004] The patent with announcement number CN214654839U discloses a timed oscillating constant temperature incubator, including an incubator body, an inner cavity is provided on one outer wall of the incubator body, and a door is rotatably connected to one side of the inner cavity on one outer wall of the incubator body through a hinge, a timing knob is provided on the other side of the outer wall of the incubator body near the inner cavity, a fixing frame is fixedly connected to the inner bottom of the inner cavity through bolts, and a first rotating cylinder is fixedly connected to one outer wall of the fixing frame through bolts. Through the rotation of the turntable and the swing of the rotating frame, the angle and direction of the culture dish can be adjusted at all times during the swinging and oscillating process, thereby realizing the oscillation of the culture surface at multiple angles and directions, and ensuring the oscillation effect.

[0005] Although the existing technology realizes rotation and oscillation during cell culture, it uses a separate drive method to make multiple culture vessels on the rotating disk rotate and oscillate synchronously. To realize the rotation and oscillation of a single culture vessel, a large number of drives are required, which greatly increases the equipment cost. Summary of the invention

[0006] The purpose of the present invention is to provide an independent rotating shaking culture device to solve the above technical problems.

[0007] To achieve the above-mentioned purpose, the present invention provides an independent rotation and shaking culture device, including a program-controlled host and an environmental adjustment system, the environmental adjustment system is electrically connected to the program-controlled host, the environmental adjustment system includes a plurality of array-distributed cabins, a rotation and shaking mechanism is arranged in the cabin, the rotation and shaking mechanism includes a driving motor, the output shaft of the driving motor is connected to a rotating shaft through a coupling, a motion conversion component is arranged on the rotating shaft, the motion conversion component includes a guide shaft installed on a fixed plate, a sliding conversion frame is slidably arranged on the guide shaft, at least one guide bearing is arranged on the inner side of the sliding conversion frame, the guide bearing is arranged in a guide groove on the circumferential side of the rotating shaft, the guide groove is wavy, the sliding conversion frame is installed on the cabin through a sealed linear bearing, the sliding conversion frame is connected to the non-rotating part of the cross ball bearing, the rotating shaft and the rotating spline are both connected to the rotating part of the cross ball bearing, the rotating spline is connected to a tray, and the tray is connected to a culture dish.

[0008] Preferably, the drive motor is provided with a position sensor, and both the drive motor and the position sensor are electrically connected to the program control host for detecting the rotation angle of the drive motor.

[0009] Preferably, the environmental regulation system also includes a temperature control subsystem, which is used to provide a stable temperature development environment for biological culture. The temperature control subsystem includes a cabin door temperature control unit and a cabin temperature control unit. The cabin door temperature control unit and the cabin temperature control unit are both electrically connected to the program control host. The cabin door temperature control unit and the cabin temperature control unit are respectively connected to a first heating plate and a second heating plate. The first heating plate is arranged on the cabin door, and the second heating plate is arranged in the cabin.

[0010] Preferably, the environmental conditioning system also includes a gas concentration control subsystem, which is used to provide a stable gas concentration development environment for biological culture. The gas concentration control subsystem includes an air intake control component, a detection component, a fan pump and a flow meter connected in sequence. The air intake control component, the detection component, the fan pump and the flow meter are all electrically connected to the program control host. According to the set corresponding gas concentration parameters, the corresponding gases are mixed to form a mixed gas. The mixed gas detects the gas concentration of the corresponding gas in the gas path through the detection component. The detection component compares the detected gas concentration value with the set gas concentration parameter and feeds it back to the program control host. The program control host replenishes the corresponding gas by adjusting the air intake control component accordingly until the gas concentration of the corresponding gas reaches the set concentration.

[0011] Preferably, the air intake control component includes an air vent, an oxygen vent, a carbon dioxide vent and a nitrogen vent, and a pressure relief return air passage is provided between the fan pump and the air intake control component.

[0012] Preferably, the oxygen concentration adjustment operation is as follows: Opening the nitrogen vent, and closing the oxygen vent and the air vent, and reducing the oxygen concentration in the gas circuit by continuously inputting nitrogen to discharge the oxygen in the gas circuit in the concentration control system; Open the oxygen vent or air vent and close the nitrogen vent to increase the oxygen concentration in the gas circuit by continuously inputting oxygen.

[0013] Preferably, the carbon dioxide adjustment operation is as follows: Close the carbon dioxide vent, open the air vent or nitrogen vent, and reduce the carbon dioxide concentration in the gas circuit by continuously introducing air or nitrogen into the gas circuit; Open the carbon dioxide vent and continue to introduce carbon dioxide into the airway to increase the carbon dioxide concentration in the airway.

[0014] Preferably, multiple groups of gas concentration control subsystems are provided, and each group of gas concentration control subsystems is connected to at least one cabin.

[0015] Therefore, the present invention adopts the above-mentioned independent rotation and shaking culture device, which has the beneficial effects of realizing independent culture of multiple chambers, providing stable temperature and gas concentration for biological culture, and realizing rotation and telescopic vibration through a motor, improving nutrient exchange efficiency, reducing equipment cost, and being more suitable for large-scale independent culture.

[0016] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of an independent rotating shaking culture device of the present invention; Figure 2 This is a schematic diagram of the structure of the rotary oscillation mechanism of the present invention; Figure 3 This is a schematic diagram of the gas concentration control subsystem of the present invention; Figure 4 This is a control principle diagram of an independent rotating shaking culture device of the present invention.

[0018] Reference numerals 1. Temperature control subsystem; 11. Door temperature control unit; 12. Cabin temperature control unit; 2. Gas concentration control subsystem; 21. Air intake control assembly; 22. Detection assembly; 23. Fan pump; 24. Flow meter; 21a. Air vent; 21b. Oxygen vent; 21c. Carbon dioxide vent; 21d. Nitrogen vent; 3. Rotary oscillation mechanism; 31. Drive motor; 32. Coupling; 33. Rotary shaft; 331. Guide groove; 34. Guide shaft; 35. Sliding conversion frame; 351. Guide bearing; 36. Sealed linear bearing; 37. Cross ball bearing; 371. Rotating part; 372. Non-rotating part; 38. Rotating spline; 39. Tray; 4. Position sensor. DETAILED DESCRIPTION

[0019] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invented product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "setting", "installation", and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0021] like Figure 1 As shown, an independent rotating shaking culture device includes a program-controlled host and an environmental adjustment system, and the environmental adjustment system is electrically connected to the program-controlled host. Figure 4 This is a control schematic diagram of an independent rotating shaking culture device.

[0022] The environmental conditioning system includes several array-distributed chambers for realizing independent environmental control of each chamber. A rotating oscillation mechanism 3 is provided in the chamber for realizing the rotation and extension oscillation of the culture dish, realizing the three-dimensional coordinate oscillation of the culture dish, and accelerating nutrient exchange. Figure 2As shown, the rotary oscillation mechanism 3 includes a driving motor 31, the output shaft of the driving motor 31 is connected to the rotating shaft 33 through a coupling 32, and a motion conversion component is arranged on the rotating shaft 33, and the motion conversion component includes a guide shaft 34 installed on a fixed plate, and a sliding conversion frame 35 is slidably arranged on the guide shaft 34, and at least one guide bearing 351 is arranged inside the sliding conversion frame 35, and the guide bearing 351 is arranged in a guide groove 331 on the circumferential side of the rotating shaft 33, and the guide groove 331 is wavy, so that the sliding conversion frame 35 is driven to reciprocate and telescopic motion along the guide shaft 34 during the rotation of the rotating shaft 33, so as to convert the rotation into linear reciprocating motion. The sliding conversion frame 35 is installed on the cabin through a sealed linear bearing 36, so that there is no air leakage between the sliding conversion frame 35 and the cabin when the reciprocating telescopic motion is realized. The sliding conversion frame 35 is connected to the non-rotating part 372 of the cross ball bearing 37, the rotating shaft 33 and the rotating spline 38 are both connected to the rotating part 371 of the cross ball bearing 37, the rotating spline 38 is connected to the tray 39, and the tray 39 is connected to the culture dish. The rotating spline 38 can maintain the rotational motion state and the linear reciprocating telescopic motion state, and the present embodiment realizes the use of a motor to drive the rotation and telescopic motion at the same time, which greatly reduces the use of the motor, is more suitable for independent culture chambers, and reduces the cost of the device. The driving motor 31 is provided with a position sensor 4, and the driving motor 31 and the position sensor 4 are both electrically connected to the program control host for detecting the rotation angle of the driving motor 31. The position sensor 4 feeds back the position signal of the driving motor 31, and the program control host realizes the high-precision rotation displacement motion and linear reciprocating telescopic motion of the tray 39 through a negative feedback control method.

[0023] The environmental regulation system also includes a temperature control subsystem 1, which is used to provide a stable temperature development environment for biological culture. The temperature control subsystem 1 includes a cabin temperature control unit 11 and a cabin temperature control unit 12. The cabin temperature control unit 11 and the cabin temperature control unit 12 are both electrically connected to the program control host. The cabin temperature control unit 11 and the cabin temperature control unit 12 are respectively connected to a first heating plate and a second heating plate. The first heating plate is arranged on the cabin door, and the second heating plate is arranged in the cabin.

[0024] like Figure 3As shown, the environmental regulation system also includes a gas concentration control subsystem 2, which is used to provide a stable gas concentration development environment for biological culture. The gas concentration control subsystem 2 includes an air intake control component 21, a detection component 22, a fan pump 23 and a flow meter 24 connected in sequence. The air intake control component 21, the detection component 22, the fan pump 23 and the flow meter 24 are all electrically connected to the program control host. According to the set corresponding gas concentration parameters, the corresponding gases are mixed to form a mixed gas. The mixed gas detects the gas concentration of the corresponding gas in the gas path through the detection component 22. The detection component 22 compares the detected gas concentration value with the set gas concentration parameter and feeds it back to the program control host. The program control host replenishes the corresponding gas by adjusting the air intake control component 21 accordingly until the gas concentration of the corresponding gas reaches the set concentration.

[0025] The air intake control component 21 includes an air vent 21a, an oxygen vent 21b, a carbon dioxide vent 21c and a nitrogen vent 21d. A pressure relief return air passage is arranged between the fan pump 23 and the air intake control component 21. When the pre-detected corresponding gas concentration is not equal to the set concentration, the pressure relief return air passage is opened to release air outwards. When the pre-detected corresponding gas concentration is equal to the set concentration, the pressure relief return air passage is closed to stop the pressure relief, thereby saving gas and improving the reflux stability. The fan pump 23 provides circulation power for the internal circulation of gas (the air intake control component 21, the detection component 22 and the circulation pipeline of the fan pump 23) and also provides power for the gas input into the cabin (the fan pump 23 is connected to the two pipelines of the air intake control component 21 and the cabin, and the output power gas provided by the fan pump 23 will be separated and flow to the air intake control component 21 and the cabin, thereby ensuring that the gas flows to the cabin to provide input power and avoiding gas waste. The gas can always be in an internal recycling state. The missing gas drawn out by the fan pump 23 and input into the cabin is continuously replenished by the external input air vent 21a, oxygen vent 21b, carbon dioxide vent 21c and nitrogen vent 21d) The fan pump 23 corrects the output flow of the fan pump 23 based on the comparison between the flow detected by the flowmeter 24 and the set output flow.

[0026] The oxygen concentration adjustment operation is as follows: The nitrogen vent 21d is opened, and the oxygen vent 21b and the air vent 21a are closed, and the oxygen concentration in the gas path is reduced by continuously inputting nitrogen to discharge the oxygen in the gas path in the concentration control system.

[0027] The oxygen vent 21b or the air vent 21a is opened, and the nitrogen vent 21d is closed, so that the oxygen concentration in the gas path is increased by continuously inputting oxygen.

[0028] The CO2 regulation operation is as follows: The carbon dioxide vent 21c is closed, and the air vent 21a or the nitrogen vent 21d is opened, and the carbon dioxide concentration in the gas path is reduced by continuously introducing air or nitrogen into the gas path.

[0029] The carbon dioxide vent 21c is opened to continuously introduce carbon dioxide into the gas path to increase the carbon dioxide concentration in the gas path.

[0030] The gas concentration control subsystem 2 is provided with multiple groups, and each group of gas concentration control subsystem 2 is connected to at least one cabin, that is, it supports the gas supply of one or more cabins according to actual needs. It should be noted that: the culture dish in this embodiment has a gas-permeable and water-impermeable membrane, so that gas can enter and exit the culture dish to achieve gas exchange.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An independent rotary shaking culture device, comprising a program-controlled host and an environmental adjustment system, wherein the environmental adjustment system is electrically connected to the program-controlled host, and characterized in that: The environmental conditioning system includes a number of cabins distributed in an array, wherein a rotational oscillation mechanism is arranged in the cabin, the rotational oscillation mechanism includes a driving motor, the output shaft of the driving motor is connected to a rotating shaft through a coupling, a motion conversion component is arranged on the rotating shaft, the motion conversion component includes a guide shaft installed on a fixed plate, a sliding conversion frame is slidably arranged on the guide shaft, at least one guide bearing is arranged on the inner side of the sliding conversion frame, the guide bearing is arranged in a guide groove on the circumferential side of the rotating shaft, the guide groove is wavy, the sliding conversion frame is installed on the cabin through a sealed linear bearing, the sliding conversion frame is connected to the non-rotating part of the cross ball bearing, the rotating shaft and the rotating spline are both connected to the rotating part of the cross ball bearing, the rotating spline is connected to a tray, and the tray is connected to a culture dish.

2. The independent rotating shaking culture device according to claim 1, characterized in that: The driving motor is provided with a position sensor, and both the driving motor and the position sensor are electrically connected to the program control host for detecting the rotation angle of the driving motor.

3. The independent rotating shaking culture device according to claim 1, characterized in that: The environmental regulation system also includes a temperature control subsystem, which is used to provide a stable temperature development environment for biological culture. The temperature control subsystem includes a cabin door temperature control unit and a cabin temperature control unit. The cabin door temperature control unit and the cabin temperature control unit are both electrically connected to the program control host. The cabin door temperature control unit and the cabin temperature control unit are respectively connected to a first heating plate and a second heating plate. The first heating plate is arranged on the cabin door, and the second heating plate is arranged in the cabin.

4. The independent rotating shaking culture device according to claim 1, characterized in that: The environmental regulation system also includes a gas concentration control subsystem, which is used to provide a stable gas concentration development environment for biological culture. The gas concentration control subsystem includes an air intake control component, a detection component, a fan pump and a flow meter connected in sequence. The air intake control component, the detection component, the fan pump and the flow meter are all electrically connected to the program control host. According to the set corresponding gas concentration parameters, the corresponding gases are mixed to form a mixed gas. The mixed gas detects the gas concentration of the corresponding gas in the gas path through the detection component. The detection component compares the detected gas concentration value with the set gas concentration parameter and feeds it back to the program control host. The program control host replenishes the corresponding gas by adjusting the air intake control component accordingly until the gas concentration of the corresponding gas reaches the set concentration.

5. The independent rotating shaking culture device according to claim 4, characterized in that: The air intake control component includes an air vent, an oxygen vent, a carbon dioxide vent and a nitrogen vent, and a pressure relief return air passage is arranged between the fan pump and the air intake control component.

6. The independent rotating shaking culture device according to claim 5, characterized in that: The oxygen concentration adjustment operation is as follows: Opening the nitrogen vent, and closing the oxygen vent and the air vent, and reducing the oxygen concentration in the gas circuit by continuously inputting nitrogen to discharge the oxygen in the gas circuit in the concentration control system; Open the oxygen vent or air vent and close the nitrogen vent to increase the oxygen concentration in the gas circuit by continuously inputting oxygen.

7. The independent rotating shaking culture device according to claim 6, characterized in that: The CO2 regulation operation is as follows: Close the carbon dioxide vent, open the air vent or nitrogen vent, and reduce the carbon dioxide concentration in the gas circuit by continuously introducing air or nitrogen into the gas circuit; Open the carbon dioxide vent and continue to introduce carbon dioxide into the airway to increase the carbon dioxide concentration in the airway.

8. The independent rotating shaking culture device according to claim 7, characterized in that: A plurality of groups of gas concentration control subsystems are provided, and each group of gas concentration control subsystems is connected to at least one cabin.

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