Efficient compound bacteria and application thereof in aquaculture
Through the cooperation of high-efficiency composite bacteria and their regulators and fillers, the COD removal and organic matter degradation problems in aquaculture are solved, the inhibition effect of blue-green algae is significantly improved, the self-purification ability of water bodies is enhanced, and the long-term improvement of water quality is achieved.
Patent Information
- Application Number
- CN202411294181.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-01-07
AI Technical Summary
The existing complex bacteria have little effect on COD removal in aquaculture, and have poor inhibitory effects on organic matter degradation and blue-green algae, which limits the efficiency of product use.
A highly effective complex bacteria, including ammonia bacteria, thiobacterium denitrogen, B. lichen, actinomycetes, yeast, Pseudomonas marsupialis, nitrifying bacteria and denitrifying bacteria, and through regulators and fillers The combination of the mixture is to prepare fine granular bacteria agents, which are used to digest the base sludge, degrade organic matter, inhibit blue-green algae, regulate the balance of sewage microbial populations, and enhance the self-purification ability of water.
It significantly improves COD removal rate, organic matter degradation rate and blue-green algae inhibition rate, maintains and improves water quality for a long time, enhances the self-purification ability of the water body, and improves product performance.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of composite bacteria, and in particular to a high-efficiency composite bacteria and application thereof in aquaculture. Background Art
[0002] In the physical treatment of aquaculture wastewater, the most commonly used are mechanical filtration and foam separation technology, both of which are used for the preliminary treatment of wastewater. The principle of mechanical filtration is barrier adsorption, which is the most basic sewage treatment method. Most of the leftover bait and aquatic product excrement in aquaculture wastewater exist in the form of suspended particles, and the use of physical filtration technology to remove them is the most convenient and effective method. In the treatment of aquaculture wastewater, mechanical filters have better filtration effects and are currently the most widely used filters; sand filters can also better remove large particles of aquaculture leftover bait and feces, and are often used in recirculating aquaculture farms. However, mechanical filtration is not effective in removing COD, BOD, N and P.
[0003] The existing composite bacteria are not effective in removing COD in aquaculture, and are not effective in degrading organic matter and inhibiting blue-green algae, which limits the efficiency of product use. Summary of the invention
[0004] In view of the defects of the prior art, the object of the present invention is to provide a high-efficiency composite bacteria and its application in aquaculture to solve the problems raised in the above background technology.
[0005] The present invention solves the technical problem by adopting the following technical solution: The present invention provides a high-efficiency composite bacteria, comprising the following raw materials in parts by weight: 20-25 parts of ammonifying bacteria, 10-15 parts of denitrifying Thiobacillus, 5-10 parts of licheniformis, 2-5 parts of actinomycetes, 2-5 parts of yeast, 3-5 parts of Rhodopseudomonas palustris, 2-5 parts of nitrifying bacteria, and 2-5 parts of denitrifying bacteria.
[0006] Preferably, the composite bacteria further comprises 2-5 parts of a regulator and 2-4 parts of a filler.
[0007] Preferably, the preparation method of the regulator is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 1-3 parts of silane coupling agent, 2-4 parts of nano calcium carbonate and 1-2 parts of carboxymethyl cellulose to 4-7 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
[0008] Preferably, the mass fraction of the sodium dodecylbenzene sulfonate solution is 4-7%.
[0009] Preferably, the silane coupling agent is silane coupling agent KH560.
[0010] Preferably, the ball milling speed of the mixing ball milling treatment is 550-750 r / min, and the mixing ball milling is performed for 1 hour.
[0011] Preferably, the preparation method of the filler is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 210-230° C. for 5-10 min, and then cooled to 55° C. at a rate of 1-3° C. / min, and kept warm; S102: 2-4 parts of lanthanum chloride solution and 1-3 parts of sodium lignin sulfonate solution are mixed evenly, and finally 1-3 parts of phosphate buffer solution are added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
[0012] Preferably, the mass fraction of the lanthanum chloride solution is 2-5%; the mass fraction of the sodium lignin sulfonate solution is 4-7%; and the pH value of the phosphate buffer solution is 5.0.
[0013] Preferably, the ultrasonic power of the ultrasonic modification treatment is 350-400W, and the ultrasonic time is 20-30min.
[0014] The invention also provides an application of the high-efficiency composite bacteria in aquaculture.
[0015] Compared with the prior art, the present invention has the following beneficial effects: The bacterial agent of the present invention is a highly efficient composite microorganism in the form of fine particles, generally containing a live bacteria count of more than 109 cfu / g (cfu / mL refers to the total number of bacterial colonies contained in each milliliter of sample), and is mainly used to digest sediment, degrade organic matter, eliminate black odor, and inhibit blue-green algae. It can adjust the balance of microbial populations in sewage water bodies, supplement the background microbial population, and restore the ecological vitality of sewage water bodies, thereby repairing and strengthening the self-purification ability of water bodies, and maintaining and improving water quality for a long time. The added regulator and filler complement each other to enhance the product performance effect. The nano calcium carbonate in the regulator is blended with the raw materials in the treating agent and then improved with sodium alginate solution. At the same time, it is coordinated and coordinated with montmorillonite and flaky talc, and then improved with raw materials such as lanthanum chloride solution, so that the performance effect of the product system is further improved. DETAILED DESCRIPTION
[0016] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0017] A highly efficient composite bacteria of this embodiment includes the following raw materials in parts by weight: 20-25 parts of ammonifying bacteria, 10-15 parts of denitrifying Thiobacillus, 5-10 parts of licheniformis, 2-5 parts of actinomycetes, 2-5 parts of yeast, 3-5 parts of Rhodopseudomonas palustris, 2-5 parts of nitrifying bacteria, and 2-5 parts of denitrifying bacteria.
[0018] The composite bacteria of this embodiment further comprises 2-5 parts of a regulator and 2-4 parts of a filler.
[0019] The preparation method of the regulator of this embodiment is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 1-3 parts of silane coupling agent, 2-4 parts of nano calcium carbonate and 1-2 parts of carboxymethyl cellulose to 4-7 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
[0020] The mass fraction of the sodium dodecylbenzenesulfonate solution of the present embodiment is 4-7%.
[0021] The silane coupling agent of this embodiment is silane coupling agent KH560.
[0022] The ball milling speed of the mixing ball milling process in this embodiment is 550-750r / min, and the mixing ball milling is performed for 1 hour.
[0023] The preparation method of the filler of this embodiment is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 210-230° C. for 5-10 min, and then cooled to 55° C. at a rate of 1-3° C. / min, and kept warm; S102: 2-4 parts of lanthanum chloride solution and 1-3 parts of sodium lignin sulfonate solution are mixed evenly, and finally 1-3 parts of phosphate buffer solution are added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
[0024] The mass fraction of the lanthanum chloride solution in this embodiment is 2-5%; the mass fraction of the sodium lignin sulfonate solution is 4-7%; and the pH value of the phosphate buffer solution is 5.0.
[0025] The ultrasonic power of the ultrasonic modification treatment in this embodiment is 350-400W, and the ultrasonic time is 20-30min.
[0026] The invention also provides an application of the high-efficiency composite bacteria in aquaculture.
[0027] Example 1. A highly efficient composite bacteria of this embodiment includes the following raw materials in parts by weight: 20 parts of ammonifying bacteria, 10 parts of denitrifying Thiobacillus, 5 parts of licheniformis, 2 parts of actinomycetes, 2 parts of yeast, 3 parts of Rhodopseudomonas palustris, 2 parts of nitrifying bacteria, and 2 parts of denitrifying bacteria.
[0028] The composite bacteria of this example also includes 2 parts of a regulator and 2 parts of a filler.
[0029] The preparation method of the regulator of this example is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 1 part of silane coupling agent, 2 parts of nano calcium carbonate and 1 part of carboxymethyl cellulose to 4 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
[0030] The mass fraction of the sodium dodecylbenzenesulfonate solution in this example is 4%.
[0031] The silane coupling agent in this example is silane coupling agent KH560.
[0032] The ball milling speed of the mixing ball milling treatment in this example is 550r / min, and the mixing ball milling is performed for 1h.
[0033] The preparation method of the filler of this example is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 210°C for 5 minutes, and then cooled to 55°C at a rate of 1°C / min, and kept warm; S102: 2 parts of lanthanum chloride solution and 1 part of sodium lignin sulfonate solution are mixed evenly, and finally 1 part of phosphate buffer solution is added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
[0034] The mass fraction of the lanthanum chloride solution in this example is 2%; the mass fraction of the sodium lignin sulfonate solution is 4%; and the pH value of the phosphate buffer solution is 5.0.
[0035] The ultrasonic power of the ultrasonic modification treatment in this example is 350 W, and the ultrasonic time is 20 min.
[0036] The invention also provides an application of the high-efficiency composite bacteria in aquaculture.
[0037] Example 2. A highly efficient composite bacteria of this embodiment includes the following raw materials in parts by weight: 25 parts of ammonifying bacteria, 15 parts of denitrifying Thiobacillus, 10 parts of licheniformis, 5 parts of actinomycetes, 5 parts of yeast, 5 parts of Rhodopseudomonas palustris, 5 parts of nitrifying bacteria, and 5 parts of denitrifying bacteria.
[0038] The composite bacteria of this example also includes 5 parts of a regulator and 4 parts of a filler.
[0039] The preparation method of the regulator of this example is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 3 parts of silane coupling agent, 4 parts of nano calcium carbonate and 2 parts of carboxymethyl cellulose to 7 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
[0040] The mass fraction of the sodium dodecylbenzenesulfonate solution in this example is 7%.
[0041] The silane coupling agent in this example is silane coupling agent KH560.
[0042] The ball milling speed of the mixing ball milling treatment in this example is 750r / min, and the mixing ball milling is performed for 1h.
[0043] The preparation method of the filler of this example is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 230°C for 10 minutes, and then cooled to 55°C at a rate of 3°C / min, and kept warm; S102: 4 parts of lanthanum chloride solution and 3 parts of sodium lignin sulfonate solution are mixed evenly, and finally 3 parts of phosphate buffer solution are added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
[0044] The mass fraction of the lanthanum chloride solution in this example is 5%; the mass fraction of the sodium lignin sulfonate solution is 7%; and the pH value of the phosphate buffer solution is 5.0.
[0045] The ultrasonic power of the ultrasonic modification treatment in this example is 400 W, and the ultrasonic time is 30 min.
[0046] The invention also provides an application of the high-efficiency composite bacteria in aquaculture.
[0047] Example 3. A highly efficient composite bacteria of this embodiment includes the following raw materials in parts by weight: 22.5 parts of ammonifying bacteria, 12.5 parts of denitrifying Thiobacillus, 7.5 parts of licheniformis, 3.5 parts of actinomycetes, 3.5 parts of yeast, 4 parts of Rhodopseudomonas palustris, 3.5 parts of nitrifying bacteria, and 3.5 parts of denitrifying bacteria.
[0048] The composite bacteria of this example also includes 3.5 parts of a regulator and 3 parts of a filler.
[0049] The preparation method of the regulator of this example is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 2 parts of silane coupling agent, 3 parts of nano calcium carbonate and 1.5 parts of carboxymethyl cellulose to 5.5 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
[0050] The mass fraction of the sodium dodecylbenzene sulfonate solution in this example is 5.5%.
[0051] The silane coupling agent in this example is silane coupling agent KH560.
[0052] The ball milling speed of the mixing ball milling treatment in this example is 600r / min, and the mixing ball milling is performed for 1h.
[0053] The preparation method of the filler of this example is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 220°C for 7.5 minutes, and then cooled to 55°C at a rate of 2°C / min, and kept warm; S102: 3 parts of lanthanum chloride solution and 2 parts of sodium lignin sulfonate solution are mixed evenly, and finally 2 parts of phosphate buffer solution are added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
[0054] The mass fraction of the lanthanum chloride solution in this example is 3.5%; the mass fraction of the sodium lignin sulfonate solution is 5.5%; and the pH value of the phosphate buffer solution is 5.0.
[0055] The ultrasonic power of the ultrasonic modification treatment in this example is 370 W, and the ultrasonic time is 25 min.
[0056] The invention also provides an application of the high-efficiency composite bacteria in aquaculture.
[0057] Comparative Example 1. The difference from Example 3 is that no regulator was added.
[0058] Comparative Example 2. The difference from Example 3 is that no treating agent is added to the conditioning agent.
[0059] Comparative Example 3. The difference from Example 3 is that no sodium alginate solution is added to the regulator.
[0060] Comparative Example 4. The difference from Example 3 is that no filler is added.
[0061] Comparative Example 5. The difference from Example 3 is that no filler liquid treatment is used in the filler.
[0062] The product performance tests of Examples 1-3 and Comparative Examples 1-5 are as follows:
[0063] It can be seen from Comparative Examples 1-5 and Example 3 that the product of Example 3 can achieve coordinated improvements in COD removal rate, organic matter degradation rate and blue-green algae inhibition rate; the present invention does not add a regulator or a filler, and the performance of the product deteriorates significantly. At the same time, no treatment agent is added to the regulator, no sodium alginate solution is added to the regulator, and no filler liquid is used to treat the filler, and the performance of the product tends to deteriorate. The product raw material obtained by the method of the present invention has the most significant product performance effect.
[0064] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.
[0065] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.
Claims
1. A highly efficient composite bacteria, characterized in that: It includes the following raw materials in parts by weight: 20-25 parts of ammonifying bacteria, 10-15 parts of denitrifying Thiobacillus, 5-10 parts of licheniformis, 2-5 parts of actinomycetes, 2-5 parts of yeast, 3-5 parts of Rhodopseudomonas palustris, 2-5 parts of nitrifying bacteria, and 2-5 parts of denitrifying bacteria.
2. According to claim 1, a highly efficient composite bacteria, characterized in that: The composite bacteria also includes 2-5 parts of a regulator and 2-4 parts of a filler.
3. The high-efficiency composite bacteria according to claim 2, characterized in that: The preparation method of the regulator is: S01: Mixing sodium alginate with a sufficient amount of sodium dodecylbenzene sulfonate solution to obtain a sodium alginate solution; S02: adding 1-3 parts of silane coupling agent, 2-4 parts of nano calcium carbonate and 1-2 parts of carboxymethyl cellulose to 4-7 parts of ethanol solvent and mixing well to obtain a treating agent; S103: The treating agent and the sodium alginate solution are mixed in a weight ratio of 2:5 and subjected to ball milling. After the ball milling is completed, the mixture is filtered and dried to obtain a regulating agent.
4. The high-efficiency composite bacteria according to claim 3, characterized in that: The mass fraction of the sodium dodecylbenzene sulfonate solution is 4-7%.
5. The high-efficiency composite bacteria according to claim 3, characterized in that: The silane coupling agent is silane coupling agent KH560.
6. The high-efficiency composite bacteria according to claim 3, characterized in that: The ball milling speed of the mixing ball milling treatment is 550-750r / min, and the mixing ball milling is performed for 1 hour.
7. The high-efficiency composite bacteria according to claim 3, characterized in that: The preparation method of the filler is: S101: montmorillonite and flaky talc are uniformly mixed in a weight ratio of 3:1, and then heat-treated at 210-230° C. for 5-10 min, and then cooled to 55° C. at a rate of 1-3° C. / min, and kept warm; S102: 2-4 parts of lanthanum chloride solution and 1-3 parts of sodium lignin sulfonate solution are mixed evenly, and finally 1-3 parts of phosphate buffer solution are added and mixed well to obtain a filler solution; S103: subjecting the product of S101 and the filler liquid to ultrasonic modification treatment in a weight ratio of 2:
5. After the ultrasonic treatment is terminated, the mixture is washed with water and dried to obtain a filler.
8. A highly efficient composite bacteria according to claim 7, characterized in that: The mass fraction of the lanthanum chloride solution is 2-5%; the mass fraction of the sodium lignin sulfonate solution is 4-7%; and the pH value of the phosphate buffer solution is 5.
0.
9. The high-efficiency composite bacteria according to claim 7, characterized in that: The ultrasonic power of the ultrasonic modification treatment is 350-400W, and the ultrasonic time is 20-30min.
10. Use of the high-efficiency composite bacteria according to any one of claims 1 to 9 in aquaculture.