Composite probiotic-fermented multi-grain metagen preparation containing medicinal and edible substances as well as preparation method and application of composite probiotic-fermented multi-grain metagen preparation

Abstract

The invention discloses a composite probiotic fermented multi-grain metabiotic preparation containing medicinal and edible substances as well as a preparation method and application of the composite probiotic fermented multi-grain metabiotic preparation, and belongs to the field of foods and health-care foods. Composite probiotics are subjected to multi-grain paste fermentation culture, and then selected specific traditional Chinese medicine raw materials are added for further fermentation to prepare a metabiotic product; the compound probiotics selected by the invention comprise two types of basic zymophyte and enhanced zymophyte, the strains are effectively proportioned, and the obtained compound probiotics are used for preparing the compound probiotics for repairing the intestinal mucosa injury, improving the intestinal health and improving the immunity of the organism, so that the compound probiotics can be used for repairing the intestinal mucosa injury, improving the intestinal health and improving the immunity of the organism. The multi-grain afterbiogen product has a synergistic effect on improving intestinal health and enhancing the immunity of the organism, and has a good application effect.

Description

Technical Field

[0001] The present invention belongs to the field of food and health food, and specifically relates to a multi-grain postbiotic preparation containing medicine and food homologous substances fermented by composite probiotics, and a preparation method and application thereof. Background Art

[0002] Ecologically beneficial Chinese medicine refers to the general term for active ingredients that have probiotic functions and are beneficial to the health of the body, which are formed by deep fermentation of microbial liquids using natural substances as raw materials and cutting-edge technologies such as microbiology and fermentation technology. The preparation of ecologically beneficial Chinese medicine includes: first selecting Chinese medicine raw materials with specific effects, and then fermenting them under specific process conditions using specific microbial strains. During the fermentation process, the active ingredients in Chinese medicine will be transformed or enriched by microorganisms, thereby improving their biological activity. At the same time, during the primary metabolism or secondary metabolism of the bacteria, some new bioactive substances or precursors with probiotic functions, such as postbiotics, will also be produced.

[0003] Postbiotics specifically refer to inanimate microorganisms and (or) their component preparations that are beneficial to the health of the host, and have multiple biological activities such as anti-inflammatory, immunomodulatory, antioxidant, anti-tumor and antibacterial. With the increasing awareness of nutrition and health care, achieving health goals through food supplements and promoting health effects through food supplements are gradually accepted and tried by the public. Therefore, in the context of people's increasing attention to health and a new understanding of the value of traditional Chinese medicine, the development of postbiotic preparations made of medicinal and edible substances fermented by probiotics will have broad development prospects by combining traditional natural raw materials and cutting-edge bioengineering methods such as microecological transformation.

[0004] At present, the development of postbiotic products in the existing technology is still in its infancy. There are few reports on postbiotic preparations fermented with compound probiotics that can simultaneously repair intestinal mucosal damage, improve intestinal health and enhance the body's immunity. It is of great significance to actively develop postbiotic products that promote intestinal health and improve the body's immunity through "food supplements and diet therapy". Summary of the invention

[0005] In view of the above-mentioned prior art, the purpose of the present invention is to provide a multi-grain postbiotic preparation containing medicinal and edible substances fermented by a composite probiotic, and a preparation method and application thereof, by fermenting the selected medicinal and edible raw materials with specific microbial strains under specific process conditions, thereby repairing intestinal mucosal damage in a "food supplement and diet therapy" manner, improving intestinal health and enhancing the body's immunity.

[0006] To achieve the above object, the present invention adopts the following technical solution: In a first aspect, the present invention provides a multi-grain postbiotic preparation containing food-and-medicine homologous substances fermented by composite probiotics, wherein the postbiotic preparation is prepared by preliminarily fermenting composite probiotics with multi-grain paste, and then adding food-and-medicine homologous substances for further fermentation; The composite probiotics are formed by mixing basic fermentation bacteria and enhanced fermentation bacteria; The basic fermentation bacteria is Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 ; The enhanced fermentation bacteria is Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09、 Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) mixed.

[0007] The Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 On July 8, 2024, it was deposited in the China Center for Type Culture Collection (CCTCC, address: Wuhan University, Wuhan, China), with the deposit number: CCTCC NO: M 20241510, and the classification name: Lactobacillus paracasei ( Lacticaseibacillus paracasei ).

[0008] The Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 The deposit number is: CCTCC No: M 20232528, and the strain has been recorded in patent CN 118530914 B.

[0009] The Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09 The deposit number is: CGMCC NO.28057. The strain has been recorded in patent CN 117343883 B.

[0010] The Pediococcus pentosaceus ( Pediococcus pentosaceus ) was purchased from the General Microbiology Center of China Microorganism Culture Collection Administration, with the number CGMCC 1.7665.

[0011] The thermophilic Streptococcus salivarius subsp. Streptococcus salivarius subsp.thermophilus ) WZA-ST57 It was deposited in China Center for Type Culture Collection (CCTCC, address: Wuhan University, Wuhan, China) on July 4, 2024, with the deposit number: CCTCC NO: M 20241481, and the classification name: Streptococcus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ). This strain has been recorded in patent CN119101635 A.

[0012] The Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) was purchased from the General Microbiology Center of China Microorganism Culture Collection Administration, with the number CGMCC1.16075.

[0013] The multi-grain paste is prepared from one or more of rice, corn, millet, soybean, sorghum, brown rice, peas, buckwheat, rye, glutinous rice, black rice, oat rice, red rice, highland barley, mung bean, cowpea, rhubarb rice, black bean, zebra bean, pocket bean, peanut and quinoa rice.

[0014] The medicine and food homologous substance is one or a mixture of red bean, coix seed, black sesame, white hyacinth bean, sword bean and Euryale ferox.

[0015] In a second aspect, the present invention provides a method for preparing the multi-grain postbiotic preparation containing medicine and food homologous substances fermented by the composite probiotics, comprising the following steps: The composite probiotics are added to the multi-grain paste, and fermented at a constant temperature of 15°C for 14-16 hours. Then, the medicinal and edible substances are added, and the fermentation is continued at a constant temperature of 15°C for 24-36 hours to terminate the fermentation. The fermented system is directly formed into solid products of different shapes by heating and baking; or the fermented system is spray-dried to make a powder.

[0016] The number of viable bacteria of each strain in the composite probiotics is (1.0-1.5)×10 6 CFU / g.

[0017] Preferably, the viable count of each strain in the composite probiotics is 1.0×10 6 CFU / g.

[0018] The mass ratio of the basic fermentation bacteria to the enhanced fermentation bacteria in the composite probiotics is 1:(1-2); The enhanced fermentation bacteria include Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09、 Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus )’s mass ratio is 1:1:1:1:1.

[0019] The mass ratio of the medicinal and edible substance to the added compound probiotics is (4-5):1.

[0020] Preferably, the vacuum degree of spray drying is 0.06-0.07 MPa, the air inlet temperature is 110-115° C., the air outlet temperature is 60-65° C., and dry powder is obtained after vacuum spray drying.

[0021] The third aspect of the present invention provides the use of the multi-grain postbiotic preparation in any one of the following (1)-(3): (1) Preparation of products for repairing intestinal mucosal damage; (2) Preparation of products to improve intestinal health; (3) Prepare products that enhance the body’s immunity.

[0022] The repair of intestinal mucosal damage and improvement of intestinal health can be specifically characterized by detecting the level of D-lactic acid in the blood of mice with damaged intestinal mucosa.

[0023] The improving the body's immunity specifically refers to: improving the immune organ index of the spleen and thymus of mice.

[0024] Beneficial effects of the present invention: 1. The present invention prepares a postbiotic product by fermenting a composite probiotic through a multi-grain paste, and then adding selected specific edible and medicinal raw materials for further fermentation. The postbiotic product has remarkable effects in repairing intestinal mucosal damage, improving intestinal health and enhancing the body's immunity, and provides a new idea for developing multi-grain postbiotic products fermented with edible and medicinal raw materials and specific microbial strains under specific process conditions through "food supplements and diet therapy".

[0025] 2. The composite probiotics selected by the present invention include two types: basic fermentation bacteria and enhanced fermentation bacteria. The basic fermentation bacteria can produce antibacterial substances such as natural antibiotics and bacteriocins, which can inhibit the growth of various pathogens. Enhanced fermentation bacteria can produce a variety of enzymes that promote digestion and absorption, so that the effective ingredients in the medicine and food homologous substances are enriched after transformation by strains. The present invention effectively proportions each strain, and the composite probiotics obtained have a synergistic effect in the preparation of multi-grain postbiotic products that repair intestinal mucosal damage, improve intestinal health and enhance the body's immunity, and the application effect is good. DETAILED DESCRIPTION

[0026] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0027] As mentioned above, by combining traditional natural raw materials, and cutting-edge bioengineering methods such as microecological transformation, the development of postbiotic preparations made of medicinal and edible substances fermented by probiotics will have broad development prospects. At present, the development of postbiotic products in the existing technology is still in its infancy. There are few reports on postbiotic preparations fermented by composite probiotics that can simultaneously repair intestinal mucosal damage, improve intestinal health and enhance the body's immunity. It is of great significance to actively develop postbiotic products that promote intestinal health and improve the body's immunity through "food supplements and diet therapy".

[0028] Based on this, the present invention discloses a multi-grain postbiotic preparation containing medicinal and edible substances fermented by a composite probiotic, and a preparation method and application thereof. The present invention prepares a multi-grain postbiotic product by fermenting and culturing an effectively proportioned composite probiotic in a multi-grain paste for a period of time, and then adding specific medicinal and edible substances for further fermentation. This product is beneficial for enhancing the intestinal mucosal immune barrier, thereby playing a role in regulating intestinal health.

[0029] D-lactic acid is a metabolite of bacterial fermentation. It can be produced by a variety of intestinal bacteria, but it is rarely absorbed under normal circumstances, and mammals do not have an enzyme system that can quickly degrade it. When the intestinal mucosa is damaged and the permeability increases, a large amount of D-lactic acid produced by bacteria in the intestine enters the blood circulation through the damaged mucosa, increasing the level of D-lactic acid in the blood. The present invention reflects the degree of intestinal mucosal damage and changes in permeability by detecting the level of D-lactic acid in the blood. The test results show that the multi-grain postbiotic preparation prepared by the present invention can repair intestinal mucosal damage and improve intestinal health. Furthermore, the spleen index and thymus index of mice were tested and it was found that the multi-grain postbiotic preparation prepared by the present invention also has the effect of improving the body's immunity.

[0030] The following detailed description is for illustrative purposes only and is intended to provide further explanation of the present application, rather than to limit the scope of the present invention.

[0031] During production, the inventor isolated a strain of bacteria from healthy breast milk and labeled it WZA-LP29 The strain was sent to the China Center for Type Culture Collection for gene sequence identification, and the identification results showed that the strain belonged to Lactobacillus paracasei ( Lacticaseibacillus paracasei ), named Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 , the strain was biologically deposited, and the deposit information is as follows: Bacterial species name: Lactobacillus paracasei Lacticaseibacillus paracasei ) WZA-LP29 ; Deposit number: CCTCC NO: M 20241510.

[0032] Classification name: Lactobacillus paracasei ( Lacticaseibacillus paracasei ); Depository: China Center for Type Culture Collection; Abbreviation of depository institution: CCTCC; Address: Wuhan University, Wuhan, China; Deposit date: July 8, 2024; The Lactobacillus rhamnosus used in the examples Lacticaseibacillus rhamnosus ) WZA-LR20 The deposit number is: CCTCC No: M 20232528, and the strain has been recorded in patent CN 118530914 B.

[0033] The Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09 The deposit number is: CGMCC NO.28057. The strain has been recorded in patent CN 117343883 B.

[0034] The Pediococcus pentosaceus ( Pediococcus pentosaceus ) was purchased from the General Microbiology Center of China Microorganism Culture Collection Administration with the order number CGMCC 1.7665.

[0035] The thermophilic Streptococcus salivarius subsp. Streptococcus salivarius subsp.thermophilus ) WZA-ST57 It was deposited in China Center for Type Culture Collection (CCTCC, address: Wuhan University, Wuhan, China) on July 4, 2024, with the deposit number: CCTCC NO: M 20241481, and the classification name: Streptococcus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ). This strain has been recorded in patent CN119101635 A.

[0036] The Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) was purchased from the General Microbiology Center of China Microorganism Culture Collection Administration with the order number CGMCC1.16075.

[0037] Example 1: Preparation of composite probiotic powder Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29、 Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09、 Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) fungus powder.

[0038] The preparation method of bacterial powder is: firstly carry out liquid deep fermentation, centrifuge the fermentation liquid continuously, and finally spray dry the precipitate obtained by centrifugation. According to this process, the number of viable bacteria prepared is 1.0×10 6 CFU / g of Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29、 Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09、 Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) fungus powder.

[0039] Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 As the basic fermentation bacteria powder. Lactiplantibacillus plantarum ) JYZC-LP09、 Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivarius subsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) The bacterial powders were mixed in a mass ratio of 1:1:1:1:1 to prepare the fortified fermented bacterial powder. The basic fermented bacterial powder and the fortified fermented bacterial powder were mixed in a mass ratio of 1:2 to prepare the composite probiotic powder.

[0040] Example 2: Multi-grain postbiotic preparation prepared by compound probiotic fermentation (1) Dissolve 10 g of sword bean powder (purchased from Baoji Liupanyun Biotechnology Co., Ltd.) in 100 mL of water and stir evenly to prepare a sword bean solution. Sterilize the sword bean solution at high temperature and set aside for use.

[0041] (2) Rice, corn, millet and soybean were mixed in a mass ratio of 1:1:1:1 and ground into grain powder. 350 g of the grain powder was dissolved in 1 L of pure water to prepare a multi-grain paste.

[0042] (3) Dissolve 2 g of the composite probiotic powder in 10 mL of PBS buffer to form a composite probiotic solution, add the composite probiotic solution to 500 mL of the multi-grain paste prepared in step (2), and ferment at 15°C for 16 h, add the sword bean solution prepared in step (1), continue to ferment at 15°C for 36 h, and then terminate the fermentation. The fermented system is vacuum spray dried (vacuum degree of 0.06-0.07 MPa, air inlet temperature of 110-115°C, air outlet temperature of 60-65°C) to prepare a multi-grain postbiotic preparation.

[0043] Example 3: Multi-grain postbiotic preparation prepared by compound probiotic fermentation (1) Grind black sesame into fine powder, weigh 5 g of black sesame powder and 5 g of Euryale ferox powder (purchased from Haozhou Xuchang Pharmaceutical Co., Ltd.), dissolve them in 100 mL of water, stir evenly to prepare a mixed solution, and sterilize the mixed solution at high temperature for later use.

[0044] (2) Peas, buckwheat, rye, and black rice were mixed in a mass ratio of 1:1:1:1 and ground into grain powder. 350 g of the grain powder was dissolved in 1 L of pure water to prepare a multi-grain paste.

[0045] (3) Dissolve 2 g of the composite probiotic powder in 10 mL of PBS buffer to form a composite probiotic solution, add the composite probiotic solution to 500 mL of the multi-grain paste prepared in step (2), and ferment at 15°C for 14 h, add the mixed solution prepared in step (1), continue to ferment at 15°C for 24 h, and then terminate the fermentation. Heat and bake the fermented system to directly shape it into a solid product in the shape of a biscuit, thereby preparing a multi-grain postbiotic preparation.

[0046] Comparative Example 1: Multi-grain postbiotic preparation prepared by fermentation with basic fermentation bacteria (1) Weigh 10 g of sword bean powder (purchased from Baoji Liupanyun Biotechnology Co., Ltd.) and dissolve it in 100 mL of water. Stir evenly to prepare the sword bean solution. Sterilize the sword bean solution at high temperature and set aside for use.

[0047] (2) Rice, corn, millet and soybean were mixed in a mass ratio of 1:1:1:1 and ground into grain powder. 350 g of the grain powder was dissolved in 1 L of pure water to prepare a multi-grain paste.

[0048] (3) Dissolve 2 g of basic fermentation bacteria powder in 10 mL of PBS buffer to prepare basic fermentation bacteria liquid, add the basic fermentation bacteria liquid to 500 mL of the multi-grain paste prepared in step (2), and ferment at 15°C for 16 h, add the sword bean solution prepared in step (1), continue to ferment at 15°C for 36 h, and then terminate the fermentation. The fermented system is vacuum spray dried (vacuum degree of 0.06-0.07 MPa, air inlet temperature of 110-115°C, air outlet temperature of 60-65°C) to prepare a multi-grain postbiotic preparation.

[0049] Comparative Example 2: Multi-grain postbiotic preparation prepared by fermentation with enhanced fermentation bacteria (1) Weigh 10 g of sword bean powder (purchased from Baoji Liupanyun Biotechnology Co., Ltd.) and dissolve it in 100 mL of water. Stir evenly to prepare the sword bean solution. Sterilize the sword bean solution at high temperature and set aside for use.

[0050] (2) Rice, corn, millet and soybean were mixed in a mass ratio of 1:1:1:1 and ground into grain powder. 350 g of the grain powder was dissolved in 1 L of pure water to prepare a multi-grain paste.

[0051] (2) Dissolve 2 g of fortified fermentation bacteria powder in 10 mL of PBS buffer to form fortified fermentation bacteria liquid, add the fortified fermentation bacteria liquid to 500 mL of the multi-grain paste prepared in step (2), and ferment at 15°C for 16 h, add the sword bean solution prepared in step (1), continue to ferment at 15°C for 36 h, and then terminate the fermentation. The fermented system is vacuum spray dried (vacuum degree of 0.06-0.07 MPa, air inlet temperature of 110-115°C, air outlet temperature of 60-65°C) to prepare a multi-grain postbiotic preparation.

[0052] Test Example 1: Detection of D-lactic acid content in mouse blood (1) Test method Forty 6-week-old male ICR mice were randomly selected and divided into four groups, control group, experimental group 1, experimental group 2, and experimental group 3, with 10 mice in each group. To construct an intestinal mucosal injury model, mice with intestinal mucosal injury will have diarrhea symptoms. Therefore, senna leaves were made into a 1:1 concentrated solution by boiling and extraction, and each mouse was gavaged at a dose of 0.2 mL / 10 g body weight to establish the model. When the mouse showed diarrhea symptoms, the model was successfully established.

[0053] The mice in the control group were fed 20 g of basal diet (30% wheat flour, 30% corn flour, 14% bran, 16% soybean meal, 5% sesame meal, and 5% fish meal) daily; the mice in the experimental group 1 were fed 10 g of basal diet + 10 g of the multi-grain postbiotic preparation prepared in Example 2 daily; the mice in the experimental group 2 were fed 10 g of basal diet + 10 g of the multi-grain postbiotic preparation prepared in Comparative Example 1; the mice in the experimental group 3 were fed 10 g of basal diet + 10 g of the multi-grain postbiotic preparation prepared in Comparative Example 2; after continuous feeding for 14 days, the D-lactic acid content in the blood of the mice was detected using a D-lactic acid detection kit (purchased from Qiyi Biotechnology Shanghai Co., Ltd.), and the average value was recorded.

[0054] (2) Test results After testing, the D-lactic acid content in the blood of mice in the control group was 6.72 mmol / L, the D-lactic acid content in the blood of mice in the experimental group 1 was 2.25 mmol / L, the D-lactic acid content in the blood of mice in the comparative example 1 group was 5.12 mmol / L, and the D-lactic acid content in the blood of mice in the comparative example 2 group was 4.78 mmol / L. The test results show that when the intestinal mucosa is damaged, taking the multi-grain postbiotic preparation prepared by the composite probiotic fermentation prepared by the present invention can effectively reduce the D-lactic acid content in the blood of mice, and has the effect of repairing intestinal mucosal damage and improving intestinal health.

[0055] Test Example 2: Mouse Immune Organ Index Detection (1) Test method: Forty 6-week-old male ICR mice were divided into 4 groups: control group, experimental group 1, experimental group 2, and experimental group 3, with 10 mice in each group.

[0056] The mice in the control group were fed 20g of basal diet (30% wheat flour, 30% corn flour, 14% bran, 16% soybean meal, 5% sesame meal, and 5% fish meal) every day; the mice in the experimental group 1 were fed 10g of basal diet + 10g of the multi-grain postbiotic preparation prepared in Example 2 every day; the mice in the experimental group 2 were fed 10g of basal diet + 10g of the multi-grain postbiotic preparation prepared in Comparative Example 1; the mice in the experimental group 3 were fed 10g of basal diet + 10g of the multi-grain postbiotic preparation prepared in Comparative Example 2; after continuous feeding for 28 days, 2 mice were randomly selected from each group of mice, a total of 8 mice, and the mice were weighed and recorded. After the body weight was recorded, the mice were killed by cervical displacement, the thymus and spleen were removed, the blood was dried with filter paper, and the weight was weighed, and the organ index was calculated. Among them, spleen index (mg / g) = spleen weight (mg) / body weight (g); thymus index (mg / g) = thymus weight (mg) / body weight (g).

[0057] (2) Test results: Table 1: Mouse immune organ index detection The test results of mouse immune organ index showed that the spleen index and thymus index of the mice in experimental group 1 were improved to varying degrees compared with those in the control group, experimental group 2, and experimental group 3. The test results showed that taking the multi-grain postbiotic preparation obtained by fermentation of the composite probiotics prepared by the present invention can improve the body's immunity (Table 1).

[0058] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification made within the spirit and principle of the present application shall be deemed to be appropriate. Equivalent replacements, improvements, etc. should all be included in the protection scope of this application.

Claims

1. A multi-grain postbiotic preparation containing food-drug homologous substances fermented by composite probiotics, characterized in that: The postbiotic preparation is prepared by initially fermenting the composite probiotics with multi-grain paste, and then adding the medicinal and edible substances for further fermentation; The composite probiotics are formed by mixing basic fermentation bacteria and enhanced fermentation bacteria; The basic fermentation bacteria is Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 ; The enhanced fermentation bacteria is Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09, Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus salivarius subsp. thermophilus ( Streptococcus salivariussubsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus ) mixed.

2. The multi-grain postbiotic preparation containing medicine and food homologous substances fermented by composite probiotics according to claim 1, characterized in that: The Lactobacillus paracasei ( Lacticaseibacillus paracasei ) WZA-LP29 The deposit number is: CCTCC NO: M 20241510, the plant lactobacillus ( Lactiplantibacillus plantarum ) JYZC-LP09 The deposit number is: CGMCC NO.28057, the rhamnosus lactobacillus ( Lacticaseibacillus rhamnosus ) WZA-LR20 The deposit number is: CCTCC No: M 20232528, the thermophilic Streptococcus salivarius subsp. Streptococcus salivariussubsp.thermophilus ) WZA-ST57 The deposit number is: CCTCC NO: M 20241481.

3. The multi-grain postbiotic preparation containing medicine and food homologous substances fermented by composite probiotics according to claim 1, characterized in that: The multi-grain paste is prepared from one or more of rice, corn, millet, soybean, sorghum, brown rice, peas, buckwheat, rye, glutinous rice, black rice, oat rice, red rice, highland barley, mung bean, cowpea, rhubarb rice, black bean, zebra bean, pocket bean, peanut and quinoa rice.

4. The multi-grain postbiotic preparation containing medicine and food homologous substances fermented by composite probiotics according to claim 1, characterized in that: The medicine and food homologous substance is one or a mixture of red bean, coix seed, black sesame, white hyacinth bean, sword bean and Euryale ferox.

5. A method for preparing a multi-grain postbiotic preparation containing food-drug homologous substances fermented by composite probiotics according to any one of claims 1 to 4, characterized in that: The steps include: The composite probiotics are added to the multi-grain paste, and fermented at a constant temperature of 15°C for 14-16 hours. Then, the food and medicine substances are added, and the fermentation is continued at a constant temperature of 15°C for 24-36 hours to terminate the fermentation. The fermented system is heated and baked to set; alternatively, the fermented system is spray-dried to make a powder.

6. The preparation method according to claim 5, characterized in that: The number of viable bacteria of each strain in the composite probiotics is (1.0-1.5)×10 6 CFU / g.

7. The preparation method according to claim 5, characterized in that: The mass ratio of the basic fermentation bacteria to the enhanced fermentation bacteria in the composite probiotics is 1:(1-2); The enhanced fermentation bacteria include Lactobacillus plantarum ( Lactiplantibacillus plantarum ) JYZC-LP09, Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) WZA-LR20 , Pediococcus pentosaceus ( Pediococcus pentosaceus ) Streptococcus salivarius subsp. thermophilus ( Streptococcus salivariussubsp.thermophilus ) WZA-ST57 and Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp bulgaricus )’s mass ratio is 1:1:1:1:

1.

8. The preparation method according to claim 5, characterized in that: The mass ratio of the medicinal and edible substance to the added compound probiotics is (4-5):

1.

9. Use of the multi-grain postbiotic preparation according to claim 1 in any one of the following (1)-(3): (1) Preparation of products for repairing intestinal mucosal damage; (2) Preparation of products to improve intestinal health; (3) Prepare products that enhance the body’s immunity.

10. The use according to claim 9, characterized in that: The improving the body's immunity specifically refers to: improving the immune organ index of the spleen and thymus of mice.

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