Dendrobium officinale powder and preparation method thereof

Abstract

The invention discloses dendrobium officinale powder and a preparation method, and mainly relates to the technical field of dendrobium. Compared with the prior art, the dendrobium officinale powder comprises felwort, honeysuckle, stevia rebaudiana, dill seed extract, medicago sativa powder and dendrobium officinale, auxiliary materials including maltodextrin, starch sodium octenylsuccinate, hydroxypropyl methyl cellulose and soybean protein isolate are added, and the powder is prepared through a spray drying technology after stirring. The bioavailability of the dendrobium officinale is improved, and multiple traditional Chinese medicine components are combined, so that the effects of resisting oxidation and relieving heatstroke are enhanced; finally, the solution can be quickly converted into dry powder through spray drying, so that the production efficiency is improved, and as a mild drying technology, spray drying is beneficial to keeping active ingredients in the raw materials not damaged, and meanwhile, the solubility and uniformity of the powder are improved.

Description

Technical Field

[0001] The invention relates to the technical field of Dendrobium, and in particular to a Dendrobium officinale powder and a preparation method thereof. Background Art

[0002] Dendrobium officinale powder is a health product made from Dendrobium officinale as the main raw material through modern processing technology. Dendrobium officinale extract is widely used as a medicinal material in traditional Chinese medicine, with the effects of nourishing yin and clearing heat, benefiting the stomach and promoting fluid production. Dendrobium officinale powder is convenient for consumers to eat directly or add to other foods. It is mainly used to enhance immunity, improve digestion, and promote healthy aging. At present, the application of Dendrobium officinale powder in the health care product market is quite mature. With the increase in consumer demand for health products, its market size continues to expand, and product forms are also constantly innovating, such as combining modern scientific and technological extraction and drying technology to improve its bioavailability and convenience.

[0003] The active ingredients such as polysaccharides and flavonoids in Dendrobium candidum have been proven to have significant antioxidant effects in terms of free radical scavenging ability, and can effectively remove free radicals in the body, slow down cell aging and tissue damage. In the prior art, research on the free radical scavenging ability of Dendrobium candidum powder has made certain progress, but in terms of the performance of relieving heatstroke symptoms, there are relatively few studies on Dendrobium candidum powder. However, the heat-clearing effect of Dendrobium candidum itself provides a theoretical basis for its application in relieving heatstroke symptoms. In the prior art, Dendrobium candidum powder is usually used in combination with other herbal ingredients with heat-clearing, detoxifying and anti-inflammatory effects to enhance its effect in relieving heatstroke symptoms. With further research on the pharmacological effects of Dendrobium candidum, its application potential in relieving heatstroke symptoms is gradually being explored and confirmed.

[0004] CN114766662A discloses a kind of Dendrobium officinale powder and its preparation method, comprising the following formula components in parts by weight: 10-15 parts of Dendrobium officinale powder, 1-2 parts of auxiliary powder, 3-6 parts of Ligustrum lucidum powder, 1-3 parts of chrysanthemum powder, 6-9 parts of corn silk powder, 9-15 parts of Chinese yam powder, and 30-60 parts of five-grain flour; the auxiliary powder is American ginseng powder; by using five-grain flour and Dendrobium officinale powder, supplemented with Ligustrum lucidum powder, chrysanthemum powder, and corn silk powder, The whole grain surface can be used as food to increase satiety when eaten, and has good nutritional ingredients to meet the needs of the human body, and has a certain effect of clearing pneumonia, wheezing, and relieving thirst. It is suitable for patients with type 3 diabetes. The auxiliary powder can play a good health-care role, and long-term use can strengthen the body. The powder has less auxiliary powder ingredients, which can avoid the situation where the auxiliary powder is too strong and affects the body. It can also reduce the edible cost of the Dendrobium officinale and facilitate its long-term consumption.

[0005] CN106579365A discloses a heatstroke prevention and cooling bagged dendrobium and a preparation method, which is composed of the following components by weight: 20-25 parts of dendrobium officinale powder, 5-10 parts of elsholtzia, 4-6 parts of reed root, 3-5 parts of lily, 4-7 parts of licorice, 4-5 parts of loquat leaf, 1-3 parts of mint leaf, 3-5 parts of watermelon rind, and 2-4 parts of food additives; the food additives are composed of the following parts by weight: 1-3 parts of wolfberry, 1-2 parts of patchouli, 2-4 parts of fresh lotus leaf, 3-4 parts of fresh bamboo leaf, and 4-5 parts of momordica grosvenori. The invention uses the dendrobium officinale powder mixed with cool plant Chinese herbal medicines, which can help people working in a high temperature environment to have the effects of cooling and quenching thirst and preventing heatstroke in the hot summer.

[0006] The solutions reported in the above patent documents cannot solve the problems of free radical scavenging and relieving heat stroke symptoms at the same time. Summary of the invention

[0007] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to improve the free radical scavenging ability and the ability to relieve heatstroke symptoms of Dendrobium officinale powder.

[0008] To achieve the above object, the present invention provides a Dendrobium officinale powder and a preparation method thereof.

[0009] The preparation method of the Dendrobium officinale powder comprises the following steps:

[0010] (1) Wash the fresh stems of Dendrobium officinale with water and wipe off the surface moisture, cut into small pieces of 0.5-1 cm in length, add them to the aqueous extractant solution, extract for 20-60 minutes at 20-40° C. and ultrasonic power of 180-200 W, centrifuge at room temperature for 10-30 minutes, and collect the supernatant, i.e., the Dendrobium officinale filtrate;

[0011] (2) Gentiana, honeysuckle, stevia, dill seed extract, and alfalfa powder are mixed, ground, sieved, and added to the Dendrobium officinale filtrate, and maltodextrin, sodium starch octenylsuccinate, hydroxypropyl methylcellulose, and soy protein isolate are added. The mixture is stirred at room temperature for 1-2 hours, and the mixture is fed into a spray drying device through a peristaltic pump. After spray drying, the powder is collected to obtain the Dendrobium officinale powder.

[0012] The aqueous extractant solution in step (1) contains ethanol and citric acid; preferably, the aqueous extractant solution contains 70-80 wt% ethanol and 0.5-1.5 wt% citric acid.

[0013] In the step (1), the weight ratio of the fresh stems of Dendrobium officinale to the aqueous solution of the extractant is 90-110:1900-2100.

[0014] In the step (2), the weight ratio of gentian, honeysuckle, stevia, dill seed extract, alfalfa powder, dendrobium officinale filtrate, maltodextrin, sodium starch octenylsuccinate, hydroxypropyl methylcellulose and soy protein isolate is 30-50: 30-50: 70-90: 40-60: 50-80: 800-1200: 250-350: 150-250: 20-40: 90-110.

[0015] The sieving in step (2) is performed through a 80-100 mesh sieve.

[0016] In the step (2), the air pressure of the spray drying device is 30-50 psi, the feed flow rate is 10-15 r / min, the feed temperature is 25-30°C, and the inlet temperature is 150-160°C.

[0017] Dendrobium officinale is a medicinal plant with high nutritional value. Its stem contains a variety of bioactive ingredients, such as polysaccharides, flavonoids and phenolic compounds. These ingredients have significant antioxidant properties and protect cells from oxidative damage by neutralizing free radicals, thereby delaying cell aging and degeneration. Dendrobium officinale also has the function of regulating the immune system, which can enhance the body's resistance and improve the body's defense against disease. The active ingredients in Dendrobium officinale can also help relieve fatigue, increase physical strength, and have a significant effect on restoring physical and mental strength.

[0018] Gentiana has good anti-inflammatory, antibacterial and heat-clearing and detoxifying properties. The ingredients such as gentiopicroside contained in it have significant anti-inflammatory effects and can help reduce the body's inflammatory response. Its antibacterial property can inhibit the growth of various bacteria and help prevent and treat infections. In the present invention, gentiana has good effects on clearing away heat and detoxifying, removing heat from the body, and relieving symptoms such as headache caused by heatstroke. At the same time, its antioxidant effect helps protect cells from damage by free radicals.

[0019] Honeysuckle contains active ingredients such as chlorogenic acid and luteolin, which have antiviral, antibacterial and anti-inflammatory effects. Ingredients such as chlorogenic acid can inhibit the growth of various viruses and bacteria and enhance the body's anti-infection ability. Honeysuckle also has the effect of clearing away heat and detoxifying, and has a positive effect on relieving symptoms such as fever, headache, and fatigue caused by heatstroke.

[0020] Stevia is a natural sweetener. Its main ingredient, stevioside, has the characteristics of high sweetness and low calories. It is suitable for diabetics and people who need to control their weight. It is also helpful in maintaining blood sugar stability during heatstroke. The antioxidant components in stevia also help protect cells from damage by free radicals.

[0021] The role of dill seed extract in preventing heatstroke and relieving heatstroke symptoms is mainly reflected in its multiple functions such as anti-oxidation, anti-inflammatory, promoting blood circulation, relieving muscle pain, sedation, and regulating electrolyte balance. Antioxidant ingredients such as volatile oils such as dillene can effectively neutralize free radicals, reduce oxidative stress in high temperature environments, and protect cells from damage; at the same time, the anti-inflammatory properties of dill seed extract can help reduce the inflammatory response caused by heatstroke, relieve symptoms such as fever, headache and muscle pain; it can also promote blood circulation by improving microcirculation, help the body dissipate heat more effectively, and lower body temperature; the sedative effect of dill seed extract can help relieve anxiety and tension during heatstroke; it may also help maintain the balance of electrolytes in the body, reduce dehydration symptoms, and enhance the body's ability to adapt to high temperature environments, thereby overall improving the individual's tolerance and resistance to high temperatures.

[0022] Alfalfa powder contains a large amount of protein, vitamins and minerals, which can provide the body with essential nutrients, help enhance immunity and promote metabolism. In terms of heatstroke prevention and relief of heatstroke symptoms, the nutrients in alfalfa powder play an important role in maintaining the normal functions of the body and enhancing physical strength.

[0023] Maltodextrin has the effect of improving the taste and stability of food. In the Dendrobium officinale powder of the present invention, maltodextrin is used as a stabilizer to help maintain the stability and consistency of the powder, so that it can maintain good quality during storage and use; sodium octenyl succinate starch is an amphoteric starch derivative containing a hydrophilic group and a lipophilic group. In the Dendrobium officinale powder of the present invention, it can improve the uniform dispersibility of each component in the powder, help ensure that all active ingredients are evenly distributed in the powder, so as to ensure that each time it is taken, a consistent effect can be obtained. Hydroxypropyl methylcellulose acts as a thickener and stabilizer, can improve the viscosity and stability of the powder, help improve the physical stability of the powder, make it not easy to agglomerate during storage and use, ensure the uniformity and consistency of the powder, and help the subsequent spray drying operation to be carried out efficiently. Soy protein isolate is a natural plant protein and a high-quality protein source rich in essential amino acids, including lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine, valine, etc. These amino acids are essential for maintaining human health; the addition of soy protein isolate improves the nutritional richness and digestibility of Dendrobium officinale powder, and helps maintain the physical and chemical stability of the powder.

[0024] Beneficial effects of the present invention:

[0025] Compared with the prior art, the Dendrobium officinale powder of the present invention includes gentian, honeysuckle, stevia, dill seed extract, alfalfa powder, Dendrobium officinale components, and then adds maltodextrin, sodium octenyl succinate starch, hydroxypropyl methylcellulose, and soy protein isolate auxiliary materials, and is made into powder by spray drying technology after stirring. Not only the bioavailability of Dendrobium officinale is improved, but also a variety of traditional Chinese medicine components are combined to enhance its antioxidant and heatstroke relief effects; finally, the solution can be quickly converted into dry powder by spray drying, thereby improving production. As a mild drying technology, spray drying helps to keep the active ingredients in the raw materials from being destroyed, while improving the solubility and uniformity of the powder. DETAILED DESCRIPTION

[0026] The parameters of the specific chemical substances used in the examples are from the following sources:

[0027] Fresh stems of Dendrobium officinale: The manufacturer is Yunnan Guangkuntang Agricultural Technology Co., Ltd., model is S-H3.

[0028] Gentian: The manufacturer is Xinfenghui Trading Department of Wuhou District, and the product number is ltao1003479147.

[0029] Honeysuckle: The manufacturer is Bozhou Baitang Pharmaceutical Co., Ltd., and the product number is 1547821.

[0030] Stevia: The manufacturer is Xinyuan Health Tea Factory in Taihe County, Anhui Province, and the product number is 1699113.

[0031] Dill seed extract: 80 mesh, grade: food grade, detection method: TLC, implementation standard: national standard, Xi'an Youshuo Biotechnology Co., Ltd.

[0032] Alfalfa powder: The manufacturer is Xi'an Anao Biotechnology Co., Ltd., and the product number is QAD-01271.

[0033] Sodium starch octenylsuccinate: The manufacturer is Shaanxi Runfeng Biotechnology Co., Ltd., model number is r-21.

[0034] Hydroxypropyl methylcellulose: The manufacturer is Jiangsu Weizhirun Biotechnology Co., Ltd., and the product number is 4657687.

[0035] Example 1

[0036] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0037] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0038] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, sieved, and added to 1000 g of Dendrobium officinale filtrate, and 300 g of maltodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of hydroxypropyl methylcellulose, and 100 g of soy protein isolate were added. The mixture was stirred at room temperature for 1 hour and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0039] Comparative Example 1

[0040] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0041] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0042] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, passed through an 80-mesh sieve, and added to 1000 g of Dendrobium officinale filtrate, 300 g of corn starch, 200 g of sodium starch octenylsuccinate, 30 g of hydroxypropyl methylcellulose, and 100 g of soy protein isolate were added, stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0043] Comparative Example 2

[0044] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0045] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0046] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, passed through an 80-mesh sieve, and added to 1000 g of Dendrobium officinale filtrate, 300 g of cyclodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of hydroxypropyl methylcellulose, and 100 g of soy protein isolate were added, stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0047] Comparative Example 3

[0048] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0049] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0050] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, sieved through an 80-mesh sieve, and added to 1000 g of Dendrobium officinale filtrate, 300 g of maltodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of carboxymethyl cellulose, and 100 g of soy protein isolate were added, stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0051] Comparative Example 4

[0052] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0053] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0054] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, passed through an 80-mesh sieve, and added to 1000 g of Dendrobium officinale filtrate, 300 g of maltodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of cellulose, and 100 g of soy protein isolate were added, stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0055] Example 2

[0056] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0057] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0058] (2) Take 1000 g of the filtrate of Dendrobium officinale, add 300 g of maltodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of hydroxypropyl methylcellulose, and 100 g of soy protein isolate, stir at room temperature for 1 hour, and feed the mixture into a spray drying device through a peristaltic pump. The air pressure of the device is 50 psi, the feed flow rate is 15 r / min, the feed temperature is 30° C., and the inlet temperature is 160° C. After spray drying, the powder is collected, 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder are added, mixed, ground, and passed through an 80-mesh sieve to obtain the Dendrobium officinale powder.

[0059] Example 3

[0060] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0061] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0062] (2) 1000 g of the filtrate of Dendrobium officinale was fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected, 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were added, mixed, ground, and passed through an 80-mesh sieve to obtain the Dendrobium officinale powder.

[0063] Example 4

[0064] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0065] (1) 100 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0066] (2) 40 g of gentian, 40 g of honeysuckle, 80 g of stevia, 50 g of dill seed extract, and 60 g of alfalfa powder were mixed, ground, sieved through an 80-mesh sieve, and added to 1000 g of the filtrate of Dendrobium officinale. The mixture was stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0067] Example 5

[0068] A method for preparing a Dendrobium officinale powder comprises the following steps:

[0069] (1) 110 g of fresh stems of Dendrobium officinale were washed with water and the surface moisture was wiped off, cut into small pieces of 1 cm in length, added to 2000 g of an aqueous extractant solution containing 75 wt % ethanol and 1 wt % citric acid, extracted at 35° C. and an ultrasonic power of 200 W for 50 minutes, centrifuged at room temperature for 20 minutes, and the supernatant was collected, i.e., the Dendrobium officinale filtrate;

[0070] (2) 50 g of gentian, 50 g of honeysuckle, 90 g of stevia, and 70 g of alfalfa powder were mixed, ground, sieved through an 80-mesh sieve, and added to 1000 g of Dendrobium officinale filtrate, 300 g of maltodextrin, 200 g of sodium starch glycolate octenylsuccinate, 30 g of hydroxypropyl methylcellulose, and 100 g of soy protein isolate were added, stirred at room temperature for 1 hour, and fed into a spray drying device through a peristaltic pump. The air pressure of the device was 50 psi, the feed flow rate was 15 r / min, the feed temperature was 30° C., and the inlet temperature was 160° C. After spray drying, the powder was collected to obtain the Dendrobium officinale powder.

[0071] Test Example 1

[0072] Take 0.5 g of the Dendrobium officinale powder obtained by the preparation method of Examples 1-5 and Comparative Examples 1-4 and dissolve it in 5 mL of water, take 0.3, 0.4, 0.5, and 0.6 mL respectively, add anhydrous ethanol to make up to 2 mL, add 2 mL of 0.1 mmol / L DPPH-anhydrous ethanol solution, keep it in a dark place for 30 min, measure the absorbance value A1 at 517 nm, replace the DPPH-anhydrous ethanol solution in the above sample solutions with anhydrous ethanol, and measure the absorbance as A2; take 2 mL of anhydrous ethanol and 2 mL of DPPH-anhydrous ethanol solution to measure the absorbance A0, and calculate the DPPH free radical scavenging rate (Y) of the sample solution; the calculation formula is as follows: Y (%) = A0-{(A1-A2) / A0}×100%.

[0073] Table 1 Free radical scavenging ability test

[0074]

[0075] From the free radical scavenging test comparison of Test Example 1, it can be seen that in Comparative Examples 1-2, compared with Example 1, the auxiliary material maltodextrin is replaced with corn starch and cyclodextrin, respectively, and in Comparative Examples 3-4, the auxiliary material hydroxypropyl methylcellulose is replaced with carboxymethyl cellulose and cellulose, respectively. The change of auxiliary materials may cause the structure, stability and bioavailability of the powder after drying to change, thereby affecting the DPPH free radical scavenging rate. Compared with Example 1, the difference between Example 2-4 and Example 1 is the order of spray drying or the step of adding auxiliary materials. In Example 2, the filtrate of Dendrobium officinale is dried first and then mixed with other components. The DPPH free radical scavenging rate is lower than that of Example 1; the step of adding auxiliary materials in Example 3-4 has the lowest DPPH free radical scavenging rate; the reason is that the order of spray drying or the step of adding auxiliary materials will affect the combination stability, encapsulation and porosity of the components of the Dendrobium officinale powder after drying, and ultimately affect the free radical scavenging rate. In Example 5, the Dendrobium officinale powder does not add dill seed extract, and the lack of the main component will also affect the DPPH free radical scavenging rate. The DPPH radical scavenging rate (96.8%) of the solution of the Dendrobium officinale powder of Example 1 is the highest compared with other examples and comparative examples. In Example 1, gentian, honeysuckle, stevia, Dendrobium officinale filtrate, and dill seed extract are mixed together and then added with auxiliary materials for spray drying, which can better maintain the stability of its biological activity. Combined with the joint action of each component, the DPPH radical scavenging rate is improved.

[0076] Test Example 2

[0077] The Dendrobium officinale powder obtained by the preparation method of Examples 1-5 was used as a sample, and 30 volunteers with mild heatstroke symptoms were selected, including 15 males and 15 females, aged between 20 and 50 years old, and were divided into 5 test groups of Examples 1-5 and 1 blank control group, with 5 people in each group.

[0078] Take 15g of the Dendrobium officinale powder obtained by the preparation method of Example 1-5 and dissolve it in a cup of 300mL of 70℃ drinking water, and the dosage is one cup at a time; all volunteers work normally in hot weather environment, and take test measures immediately after the symptoms of mild heat stroke appear and move to a cool and ventilated place. The volunteers in the blank control group only sit and rest in a cool and ventilated place for 2 hours. Symptoms of mild heat stroke usually include dizziness, headache, flushed face, thirst, heavy sweating, general fatigue, palpitations, rapid pulse, slightly elevated body temperature, etc. Due to the different ages, physical conditions and metabolic rates of the volunteers, the absorption and metabolism of the Dendrobium officinale powder in the body may be affected; therefore, the 2 hours after drinking are used as the test duration. If the symptoms of heat stroke disappear completely within 2 hours, it is called that the symptoms of heat stroke are well relieved; converted to the relief rate (%), summarized as described in Table 2.

[0079] Table 2 Heat stroke relief rate test

[0080] Example 1 Example 2 Example 3 Example 4 Example 5 blank Heat stroke relief rate 100.0％ 80.0％ 60.0％ 60.0％ 60.0％ 40.0％

[0081] In test example 2, the heat stroke relief rate test was carried out for examples 1-5 and the blank control group. In example 1, gentian, honeysuckle, stevia, Dendrobium officinale filtrate, and dill seed extract were mixed together and then added with auxiliary materials for spray drying, which can better maintain the stability of its biological activity, make each component better combined, better play the role of each component, and can completely eliminate the symptoms of heat stroke within 2 hours. However, examples 2-4 only changed the auxiliary materials and timing of spray drying, which ultimately affected the physicochemical properties and in vivo behavior of Dendrobium officinale powder, thereby affecting the bioavailability of each component, resulting in a decrease in the heat stroke relief rate. In example 5, no dill seed extract was added to the components, and the relief rate for heat stroke was lower than that of examples 1-2, but still higher than the heat stroke symptom relief rate of the blank control group, indicating that in the absence of taking Dendrobium officinale powder, only by resting and avoiding high temperature environment, the symptoms of heat stroke can be relieved to a certain extent within 2 hours, but the relief effect is not obvious.

[0082] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A method for preparing a powder of Dendrobium officinale, characterized in that: The following steps are involved: (1) Wash the fresh stems of Dendrobium officinale with water and wipe off the surface moisture, cut into small pieces of 0.5-1 cm in length, add them to the aqueous extractant solution, extract for 20-60 minutes at 20-40° C. and ultrasonic power of 180-200 W, centrifuge at room temperature for 10-30 minutes, and collect the supernatant, i.e., the Dendrobium officinale filtrate; (2) Gentiana, honeysuckle, stevia, dill seed extract, and alfalfa powder are mixed, ground, sieved, and added to the Dendrobium officinale filtrate, and maltodextrin, sodium starch octenylsuccinate, hydroxypropyl methylcellulose, and soy protein isolate are added. The mixture is stirred at room temperature for 1-2 hours, and the mixture is fed into a spray drying device through a peristaltic pump. After spray drying, the powder is collected to obtain the Dendrobium officinale powder.

2. The method for preparing the Dendrobium officinale powder according to claim 1, wherein The aqueous extractant solution in step (1) contains ethanol and citric acid.

3. The method for preparing the Dendrobium officinale powder according to claim 2, wherein: The extractant aqueous solution in step (1) contains 70-80 wt % ethanol and 0.5-1.5 wt % citric acid.

4. The method for preparing the Dendrobium officinale powder according to claim 1, wherein In the step (1), the weight ratio of the fresh stems of Dendrobium officinale to the aqueous solution of the extractant is 90-110:1900-2100.

5. The method for preparing the Dendrobium officinale powder according to claim 1, characterized in that: In the step (2), the weight ratio of gentian, honeysuckle, stevia, dill seed extract, alfalfa powder, dendrobium officinale filtrate, maltodextrin, sodium starch octenylsuccinate, hydroxypropyl methylcellulose and soy protein isolate is 30-50: 30-50: 70-90: 40-60: 50-80: 800-1200: 250-350: 150-250: 20-40: 90-110.

6. The method for preparing the Dendrobium officinale powder according to claim 1, characterized in that: The sieving in step (2) is performed through a 80-100 mesh sieve.

7. The method for preparing the Dendrobium officinale powder according to claim 1, characterized in that: In the step (2), the air pressure of the spray drying device is 30-50 psi, the feed flow rate is 10-15 r / min, the feed temperature is 25-30°C, and the inlet temperature is 150-160°C.

8. A Dendrobium officinale powder, characterized in that: Prepared by the method according to any one of claims 1 to 7.

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