Application of pitaya branch polysaccharide in intervention of adipogenesis

Abstract

The invention relates to application of pitaya branch polysaccharide in intervention of adipogenesis, and relates to the technical field of biology. According to the application of the pitaya branch polysaccharide in the adipogenesis intervention product, the pitaya branch polysaccharide is an extract obtained from pitaya branches through a water extraction method, adipogenesis can be remarkably reduced, and feasibility is provided for reducing fat and losing weight.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to application of pitaya branch polysaccharide in intervening lipogenesis. Background Art

[0002] At present, about 40% of adults in the world are overweight or obese, and the trend is spreading rapidly. Obesity has become a global "epidemic". The energy intake of obese patients is greater than their consumption, which is mainly manifested by excessive fat accumulation and lipid metabolism disorders. At the same time, they are often accompanied by diabetes, hyperinsulinemia and other diseases that cause insulin resistance, causing extremely serious economic and social burdens worldwide. At present, commonly used lipid-lowering drugs such as statins, fibrates, and orlistat have certain lipid-lowering effects, but long-term use will cause adverse reactions such as nausea, bloating, muscle aches, and high liver toxicity, and the effect on secondary lipid metabolism disorders is not ideal.

[0003] Caenorhabditis elegans is a multicellular model organism whose somatic cell developmental lineage has been clearly studied. Caenorhabditis elegans is the first animal to have its genome completely sequenced, and of the 20,000 genes in nematodes, approximately 15% to 30% are essential. Approximately 40% of genes associated with human diseases are phylogenetically identical to genes in nematodes. Therefore, the biological information obtained from studying nematodes is also applicable to humans and higher animals, and because of its short lifespan (about 3 weeks), ease of observation, highly conserved genetic information, and the visualization advantage of fluorescently labeled organs, Caenorhabditis elegans is an ideal model for drug screening. Summary of the invention

[0004] In view of the above problems, the present invention provides an application of pitaya branch polysaccharide in a product for intervening fat production. The pitaya branch polysaccharide can significantly reduce fat production, providing feasibility for lipid-lowering and weight-loss.

[0005] In order to achieve the above-mentioned purpose, the present invention provides an application of pitaya branch polysaccharide in a product for intervening lipogenesis. The pitaya branch polysaccharide is an extract obtained from pitaya branches by water extraction.

[0006] In one embodiment, the application includes using the pitaya branch polysaccharide to prepare a product for intervening in adipogenesis; the product includes a medicine or a food.

[0007] In one embodiment, the water extraction method comprises the following steps: soaking pitaya branches in a solvent, removing the solvent to obtain pretreated pitaya branches, adding water, extracting at high temperature and high pressure, taking the supernatant, concentrating to obtain a concentrated solution, adding ethanol, precipitating with alcohol, centrifuging, taking the precipitate, dissolving, removing impurities, dialyzing, and freeze-drying to obtain an extract.

[0008] The water extraction method has simple process, few steps, high production efficiency, low cost, and safe and convenient operation.

[0009] In one embodiment, the solvent comprises at least one of petroleum ether and ethanol, the soaking time is 3-6 hours, the ratio of the pretreated pitaya branches to the water is 1g: (18-25) mL, the temperature of the high temperature and high pressure extraction is 120-130°C, the time of the high temperature and high pressure extraction is 25-35min, the volume of ethanol in the ethanol adding step is 4-7 times that of the concentrated solution, and the alcohol precipitation time is 20-30h.

[0010] In one embodiment, the pitaya branch polysaccharide is a polysaccharide from red pitaya branches.

[0011] In one embodiment, the product is used for reducing fat and losing weight.

[0012] In one embodiment, the food comprises health food.

[0013] In one embodiment, the health food comprises a health drink.

[0014] In one embodiment, the drug further comprises a pharmaceutically acceptable excipient;

[0015] The food also includes food additives.

[0016] In one embodiment, the dosage form of the drug includes an injectable preparation or an oral preparation.

[0017] In one embodiment, the oral preparation includes tablets, capsules, powders, pills or oral liquid preparations.

[0018] In one embodiment, the application includes oral administration or injection of the drug, or oral administration of the food, and the working concentration of the pitaya branch polysaccharide is less than 80 μg / mL.

[0019] The working concentration is the concentration at which the above-mentioned ingredients can achieve the expected effect when used. It is understandable that those skilled in the art can prepare a mother solution or reserve solution with a higher concentration when preparing the above-mentioned medicine or food, and then dilute it when used. The mother solution or reserve solution and its concentration are all within the protection scope of the present invention.

[0020] In one embodiment, the working concentration of the pitaya branch polysaccharide is ≤60 μg / mL.

[0021] In one embodiment, the working concentration of the pitaya branch polysaccharide is ≤40 μg / mL.

[0022] The present invention also provides a product for reducing blood lipids, which is a medicine or a food, and the medicine or the food comprises the pitaya branch polysaccharide in the application.

[0023] In one embodiment, the medicine further includes pharmaceutically acceptable excipients; the food further includes food additives.

[0024] In one embodiment, the auxiliary material includes at least one of an excipient, a lubricant, an antioxidant, a preservative, a binder, a filler, or a thickener.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The invention discloses an application of pitaya branch polysaccharide in intervening fat generation. The pitaya branch polysaccharide is taken from pitaya branches, the raw materials are easily available, the price is cheap, and there is no toxic side effect, and the pitaya branch polysaccharide can be used safely for a long time. The pitaya branch polysaccharide has obvious pharmacological effects, can significantly reduce fat generation, and provides feasibility for lipid-lowering and weight loss. The polysaccharide is very valuable for developing into lipid-lowering and weight loss related products. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a graph showing the effect of different doses of pitaya branch polysaccharide on the fluorescence intensity of fat accumulation in Caenorhabditis elegans;

[0028] Figure 2 This is a graph showing the effects of different doses of pitaya branch polysaccharide on the relative content of fat in the body of Caenorhabditis elegans. The pitaya branch polysaccharide dose groups were compared with the negative control group (Control). * P<0.05, ** P<0.01, *** P<0.001. DETAILED DESCRIPTION

[0029] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0031] source:

[0032] Unless otherwise specified, the reagents, materials, and equipment used in this example are all commercially available; the experimental methods are all conventional experimental methods in the art unless otherwise specified.

[0033] Example 1

[0034] Extraction of polysaccharides from pitaya branches.

[0035] The polysaccharide from the pitaya branches is extracted by water extraction, and the specific steps are as follows:

[0036] 1. Cut the red pitaya branches into slices and dry them under reduced pressure until the quality is constant;

[0037] 2. Soak and extract with petroleum ether and 95% ethanol for 4 hours to remove fat-soluble components such as pigments and small molecular components, and evaporate the solvent to obtain the pretreated sample;

[0038] 3. Accurately weigh the pretreated sample, add water at a solid-liquid ratio of 1:20, place it in a pressure cooker extraction device, and extract at 121°C for 30 minutes;

[0039] 4. Concentrate the supernatant to 1 / 5 of the original volume, add 5 times the volume of anhydrous ethanol, precipitate for 24 hours, and centrifuge to obtain the precipitate;

[0040] 5. Dissolve the precipitate with an appropriate amount of deionized water to remove insoluble proteins, and dialyze with deionized water to remove small molecule impurities and freeze-dried polysaccharide components.

[0041] Example 2

[0042] Effects of polysaccharides from pitaya branches on adipogenesis in Caenorhabditis elegans.

[0043] The experiment in this example was commissioned to Shanghai Model Organisms Technology Co., Ltd.

[0044] 1. Nematode culture conditions.

[0045] The wild type of Caenorhabditis elegans N2 was provided by the Caenorhabditis Genetics Center (CGC) of the University of Minnesota, USA. The nematodes were cultured on NGM (Nematode Growth Medium) solid medium containing Escherichia coli OP50 at a culture temperature of 20° C. and a humidity of 40-60%.

[0046] 2. Sample information.

[0047] The sample "Pitaya branch polysaccharide" was dissolved in ddH2O, and the experimental concentrations were 20 μg / mL, 40 μg / mL and 80 μg / mL.

[0048] 3. Experimental method.

[0049] At 20℃, wild-type N2 nematodes were synchronized and cultured for 3 days. After developing into adults, they were picked into 3cm flat dishes containing 2mL LCM liquid culture medium in each well. The culture medium contained appropriate concentrations of drugs, and there were about 15-20 worms per dish. The nematodes were cultured in the drug until the third day, and the difference in fat content between the drug-treated group and the control group was detected. After staining in Nile red for 3 hours, they were transferred to a 2% agar pad slide and observed under a fluorescence microscope. FUDR (400uM) was added to the culture medium to inhibit the production of offspring in nematodes.

[0050] 4. Statistical analysis.

[0051] Imgge J was used to perform relative quantitative analysis on the fluorescence images after Nile red staining, and T-test was used for comparison between the two groups.

[0052] 5. Experimental results.

[0053] like Figure 1 , 2 As shown, the nematodes were cultured in liquid culture medium with concentrations of 20 μg / mL, 40 μg / mL and 80 μg / mL of the sample "Pitaya Branch Polysaccharide" for 3 days. After Nile red staining, the fat content of the nematodes cultured with the sample "Pitaya Branch Polysaccharide" at concentrations of 20 μg / mL and 40 μg / mL was significantly lower than that of the control group, and the fat content of the nematodes cultured with a concentration of 80 μg / mL was not significantly different from that of the control group.

[0054] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0055] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An application of pitaya branch polysaccharide in a product for intervening lipogenesis, characterized in that: The pitaya branch polysaccharide is an extract obtained from the pitaya branch by water extraction.

2. The use according to claim 1, characterized in that: The application includes using the pitaya branch polysaccharide to prepare products for intervening in fat generation; the products include medicines or foods.

3. The use according to claim 2, characterized in that: The drug also includes pharmaceutically acceptable excipients; The food also includes food additives.

4. The use according to claim 2, characterized in that: The dosage form of the drug includes an injection preparation or an oral preparation.

5. The use according to claim 4, characterized in that: The oral preparations include tablets, capsules, powders, drop pills or oral liquid preparations.

6. The use according to claim 2, characterized in that: The application includes oral administration or injection of the drug, or oral administration of the food, and the working concentration of the pitaya branch polysaccharide is less than 80 μg / mL.

7. A product for reducing lipids, characterized in that: The product is a medicine or a food, and the medicine or the food comprises the pitaya branch polysaccharide used in any one of claims 1 to 6.

8. The product according to claim 7, characterized in that The medicine also includes pharmaceutically acceptable excipients; the food also includes food additives.

9. The product according to claim 8, characterized in that The auxiliary materials include at least one of excipients, lubricants, antioxidants, preservatives, adhesives, fillers, or thickeners.

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