Skin care composition containing white truffle and plant exosome and application of skin care composition

Abstract

The invention relates to a skin care composition containing white truffle and plant exosome and application thereof. The skin care composition containing white truffle and plant exosome comprises white truffle extract, chlorella exosome, DNA sodium, superoxide dismutase and fermentation product filtrate, the fermentation product filtrate comprises any one or a combination of at least two of lactobacillus / soybean milk fermentation product filtrate, lactobacillus / mung bean seed extract / sodium glutamate fermentation product filtrate, bifidobacterium fermentation product filtrate or saccharomycetes / rice fermentation product filtrate. According to the invention, the white truffle extract, the chlorella exosome, the DNA sodium, the superoxide dismutase and the fermentation product filtrate are creatively combined, and the composition has unexpected synergistic interaction in the aspects of inhibiting melanin synthesis, accelerating melanin metabolism and resisting oxidation and aging. The composition is mild in performance and free of irritation and toxic and side effects, and can be applied to cosmetic products more safely.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition comprising white truffle and plant exosomes and an application thereof. Background Art

[0002] With the development and progress of society, people are paying more and more attention to the external manifestations of their personal image and health status. Whitening can make the skin look brighter, which is beneficial to improving one's own beauty and self-confidence, and can also make people look younger. Therefore, more consumers have further demand for whitening, and the market demand for whitening cosmetics is growing.

[0003] CN118662412A discloses a whitening and anti-aging raw material, which comprises, by mass ratio: 6-15 grams of licorice extract, 3-8 grams of arbutin, 0.5-5 grams of adenosine, 2-10 grams of collagen, 0.5-5 grams of coenzyme Q10, 1-6 grams of magnesium vitamin C phosphate, 0.5-5 grams of green tea polyphenols, 30-60 ml of fermentation liquid, 50-100 grams of nanocarriers, 80-200 grams of purified water, 800-1200 grams of cosmetic matrix, and 1-5 grams of zinc oxide. The licorice extract and arbutin are prepared by mixing them into a The clean raw material powder is mixed with solvent water, and then heated to 100-120°C, and then filtered, concentrated, purified and dried to finally obtain the corresponding extract. The invention can effectively reduce melanin, brighten skin tone and whiten skin through the combined effects of licorice extract, arbutin and magnesium vitamin C phosphate; it can provide excellent moisturizing effect through sodium hyaluronate and fermentation liquid to maintain the moisture and elasticity of the skin; it can reduce fine lines and wrinkles and enhance the elasticity and firmness of the skin through adenosine, collagen and coenzyme Q10.

[0004] CN118697673A discloses a whitening composition and its preparation method, which is composed of the following components by weight: 30-40 parts of anthocyanidin extract, 15-25 parts of American ginseng extract, 10-20 parts of cordyceps extract, 20-40 parts of white tea extract, 140-220 parts of deionized water, 30-70 parts of lemon extract, and 15-35 parts of auxiliary agent. The main components of the invention are anthocyanidin extract, American ginseng extract, cordyceps extract and white tea extract, which are less harmful to the human body, not only have whitening effect, but also have good antioxidant ability, can delay aging, and American ginseng extract can replenish the moisture lost on the face to reduce the occurrence of dry skin, and white tea extract can reduce the formation and deposition of melanin, thereby reducing the generation of freckles.

[0005] Although the above-mentioned cosmetics all have whitening and antioxidant effects, due to the barrier function of human skin itself, it is difficult for general whitening active ingredients to truly penetrate deep into the skin to exert their effects. Therefore, it is necessary to develop a whitening and antioxidant product with more obvious effects and greater safety to meet the needs of consumers. Summary of the invention

[0006] In view of the deficiencies in the prior art, an object of the present invention is to provide a skin care composition comprising white truffle and plant exosomes and applications thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care composition comprising white truffle and plant exosomes, wherein the skin care composition comprising white truffle and plant exosomes comprises white truffle extract, Chlorella exosomes, sodium DNA, superoxide dismutase and fermentation product filtrate;

[0009] The fermentation product filtrate includes any one of lactobacillus / soymilk fermentation product filtrate, lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, bifid yeast fermentation product filtrate or yeast / rice fermentation product filtrate or a combination of at least two thereof.

[0010] The white truffle extract in the composition of the present invention is rich in amino acids and vitamins, which can deeply nourish the skin, enhance the whitening and elasticity of the skin, and can also resist the damage of free radicals and delay skin aging; the chlorella exosomes can promote the synthesis of collagen and elastin, thereby improving the brightness and uniformity of the skin, and it also has excellent antioxidant effect; the DNA sodium can promote the metabolism and regeneration of epidermal cells, improve skin dullness, brighten skin tone, delay skin aging, and make the skin shiny; the superoxide dismutase is a powerful antioxidant enzyme, which can neutralize superoxide free radicals, and reduce the effect of superoxide free radicals on the synthesis of melanin by melanocytes by removing oxygen free radicals in cells, thereby playing a whitening role; the fermentation product filtrate can inhibit the production of melanin, thereby reducing skin spots and dullness, making the skin look brighter and more uniform, and the five are used in combination to have a synergistic effect, and the effective ingredients can better penetrate into the skin, so that the whitening and antioxidant effects of the composition can be significantly enhanced.

[0011] Preferably, the skin care composition comprising white truffle and plant exosomes comprises, by weight, 0.5-2 parts of white truffle extract, 1-3 parts of Chlorella exosomes, 1-2 parts of DNA sodium, 3-5 parts of superoxide dismutase and 5-10 parts of fermentation product filtrate.

[0012] The amount of the white truffle extract added to the skin care composition comprising white truffle and plant exosomes of the present invention can be 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0013] The amount of the Chlorella exosomes added can be 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts or 2.9 parts, etc.;

[0014] The amount of sodium DNA added can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0015] The amount of superoxide dismutase added can be 3.1 parts, 3.3 parts, 3.5 parts, 3.7 parts, 3.9 parts, 4 parts, 4.1 parts, 4.3 parts, 4.5 parts, 4.7 parts or 4.9 parts, etc.;

[0016] The added amount of the fermentation product filtrate can be 5.2 parts, 5.5 parts, 5.8 parts, 6 parts, 6.2 parts, 6.5 parts, 6.8 parts, 7 parts, 7.2 parts, 7.5 parts, 7.8 parts, 8 parts, 8.2 parts, 8.5 parts, 8.8 parts, 9 parts, 9.2 parts, 9.5 parts or 9.8 parts, etc.

[0017] Preferably, the fermentation product filtrate is a combination of lactobacillus / soymilk fermentation product filtrate, lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, bifid yeast fermentation product filtrate and yeast / rice fermentation product filtrate.

[0018] Preferably, the mass ratio of the lactobacillus / soymilk fermentation product filtrate, the lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is (1-2):(0.1-1):(1-3):(1-2).

[0019] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0020] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0021] "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0022] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0023] Preferably, the skin care composition comprising white truffle and plant exosomes also includes protein.

[0024] Preferably, the skin care composition comprising white truffle and plant exosomes also includes 1-4 parts of protein by weight, for example, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts or 3.8 parts, etc.

[0025] Preferably, the protein comprises recombinant human collagen and / or hydrolyzed elastin.

[0026] Preferably, the protein comprises recombinant human collagen and hydrolyzed elastin.

[0027] Preferably, the mass ratio of the recombinant human collagen to the hydrolyzed elastin is 1:(0.5-2), for example, it can be 1:0.6, 1:0.8, 1:1, 1:1.2, 1:1.4, 1:1.6 or 1:1.8, etc.

[0028] In the present invention, by adding an appropriate amount of recombinant human collagen and hydrolyzed elastin to the composition, the collagen required by the skin can be further supplemented, the fibrous tissue of the skin can be reorganized, and cell metabolism can be promoted, thereby making the composition have a better whitening and antioxidant effect.

[0029] Preferably, the skin care composition comprising white truffle and plant exosomes also includes a polypeptide.

[0030] Preferably, the skin care composition comprising white truffles and plant exosomes also includes 1-5 parts of polypeptides by weight, for example, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts or 4.8 parts, etc.

[0031] Preferably, the polypeptide comprises any one of carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 or palmitoyl tetrapeptide-7, or a combination of at least two thereof.

[0032] Preferably, the polypeptides include carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7.

[0033] Preferably, the mass ratio of carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 is (2-4):(0.5-1.5):(1-2):(1-2):(1-2).

[0034] Wherein "2-4" can be 2.1, 2.3, 2.5, 2.7, 2.9, 3, 3.1, 3.3, 3.5, 3.7 or 3.9, etc.;

[0035] “0.5-1.5” can mean 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.;

[0036] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0037] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0038] The third "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0039] The polypeptide of the present invention can promote the penetration of active ingredients into the skin, thereby activating epidermal cells. The polypeptide can also neutralize free radicals, promote the growth and renewal of skin cells, and make the skin firmer and more elastic. The combination of carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 selected in the present invention can make the above effects more significant.

[0040] In a second aspect, the present invention provides a method for preparing a skin care composition comprising white truffle and plant exosomes as described in the first aspect, the preparation method comprising the following steps: mixing and stirring white truffle extract, Chlorella exosomes, sodium DNA, superoxide dismutase and fermentation product filtrate to obtain the skin care composition comprising white truffle and plant exosomes.

[0041] Preferably, the preparation method comprises:

[0042] (1) mixing white truffle extract, Chlorella exosomes, fermentation product filtrate and water to obtain solution A;

[0043] (2) mixing the DNA sodium, superoxide dismutase and the solution A obtained in step (1) to obtain a solution B;

[0044] (3) Finally, the protein and polypeptide are mixed with the solution B obtained in step (2) to obtain the skin care composition containing white truffle and plant exosomes.

[0045] Preferably, the mixing temperature in step (1) is 45-55°C (for example, 46°C, 48°C, 50°C, 52°C or 54°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 1-2h (for example, 1.1h, 1.3h, 1.5h, 1.7h or 1.9h, etc.), and the stirring speed is 1000-2000rpm (for example, 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0046] Preferably, the mixing temperature in step (2) is 45-55°C (for example, 46°C, 48°C, 50°C, 52°C or 54°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 0.5-1h (for example, 0.6h, 0.7h, 0.8h, 0.9h or 1h, etc.), and the stirring speed is 1000-2000rpm (for example, 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0047] Preferably, the mixing temperature in step (3) is 40-50°C (for example, 40°C, 42°C, 45°C, 48°C or 50°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 1-2h (for example, 1.1h, 1.3h, 1.5h, 1.7h or 1.9h, etc.), and the stirring speed is 1000-2000rpm (for example, 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0048] In a third aspect, the present invention provides a use of a skin care composition comprising white truffle and plant exosomes as described in the first aspect in the preparation of cosmetics.

[0049] Preferably, the cosmetics include any one of essence, essence milk, face cream, face cleanser or lotion.

[0050] Preferably, the mass percentage of the skin care composition comprising white truffle and plant exosomes in the cosmetic is 1-20%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% or 19%, etc.

[0051] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] The present invention creatively combines white truffle extract, chlorella exosomes, sodium DNA, superoxide dismutase, and fermentation product filtrate to form a composition with whitening and antioxidant effects. The five combinations of white truffle extract, chlorella exosomes, sodium DNA, superoxide dismutase, and fermentation product filtrate in the composition are scientifically matched, and have unexpected synergistic effects in inhibiting melanin synthesis, accelerating melanin metabolism, and anti-oxidation and anti-aging. In addition, the composition has mild performance, no irritation, no toxic side effects, and can be more safely applied to cosmetic products. DETAILED DESCRIPTION

[0054] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0055] The following materials and comparative examples are as follows:

[0056]

[0057]

[0058] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0059] Example 1

[0060] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the skin care composition comprising white truffle and plant exosomes comprises, by weight, 1.5 parts of white truffle extract, 2 parts of Chlorella exosomes, 1.5 parts of sodium DNA, 4 parts of superoxide dismutase, 8 parts of fermentation product filtrate, 3 parts of protein, 3 parts of polypeptide, and water is added to 50 parts;

[0061] The fermentation product filtrate is a lactobacillus / soy milk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, a bifid yeast fermentation product filtrate and a yeast / rice fermentation product filtrate in a mass ratio of 1.5:0.5:2:1.5;

[0062] The proteins are recombinant human collagen and hydrolyzed elastin in a mass ratio of 1:1;

[0063] The polypeptides are carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 3:1:1.5:1.5:1.5.

[0064] The preparation method of the skin care composition containing white truffle and plant exosomes is:

[0065] (1) mixing white truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 50° C. and 1500 rpm for 1.5 h to obtain solution A;

[0066] (2) mixing the sodium DNA and superoxide dismutase with the solution A obtained in step (1), and stirring at 50° C. and 1500 rpm for 0.8 h to obtain a solution B;

[0067] (3) Finally, the protein and polypeptide are mixed with the solution B obtained in step (2), and stirred at 45° C. and 1500 rpm for 1.5 h to obtain the skin care composition containing white truffle and plant exosomes.

[0068] Example 2

[0069] This embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the skin care composition comprising white truffle and plant exosomes comprises, by weight, 2 parts of white truffle extract, 1.5 parts of Chlorella exosomes, 2 parts of DNA sodium, 3 parts of superoxide dismutase, 10 parts of fermentation product filtrate, 2 parts of protein, 2 parts of polypeptide, and water is added to 50 parts;

[0070] The fermentation product filtrate is a lactobacillus / soy milk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, a bifid yeast fermentation product filtrate, and a yeast / rice fermentation product filtrate in a mass ratio of 2:0.3:3:1;

[0071] The proteins are recombinant human collagen and hydrolyzed elastin in a mass ratio of 2:1;

[0072] The polypeptides are carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 4:0.5:2:1:2.

[0073] The preparation method of the skin care composition containing white truffle and plant exosomes is:

[0074] (1) mixing white truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 55° C. and 1200 rpm for 2 h to obtain solution A;

[0075] (2) mixing the DNA sodium and superoxide dismutase with the solution A obtained in step (1), and stirring at 45° C. and 2000 rpm for 0.5 h to obtain a solution B;

[0076] (3) Finally, the protein and the polypeptide are mixed with the solution B obtained in step (2), and stirred at 40° C. and 1000 rpm for 2 h to obtain the skin care composition containing white truffles and plant exosomes.

[0077] Example 3

[0078] This embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the skin care composition comprising white truffle and plant exosomes comprises, by weight, 1 part of white truffle extract, 2.5 parts of Chlorella exosomes, 1 part of DNA sodium, 5 parts of superoxide dismutase, 5 parts of fermentation product filtrate, 4 parts of protein, 5 parts of polypeptide, and water is added to 50 parts;

[0079] The fermentation product filtrate is a lactobacillus / soy milk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, a bifid yeast fermentation product filtrate and a yeast / rice fermentation product filtrate in a mass ratio of 1:1:1:2;

[0080] The proteins are recombinant human collagen and hydrolyzed elastin in a mass ratio of 1:2;

[0081] The polypeptides are carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 2:1.5:1:2:1.

[0082] The preparation method of the skin care composition containing white truffle and plant exosomes is:

[0083] (1) mixing white truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 45° C. and 2000 rpm for 1 h to obtain solution A;

[0084] (2) mixing the sodium DNA and superoxide dismutase with the solution A obtained in step (1), and stirring at 55° C. and 1000 rpm for 1 h to obtain a solution B;

[0085] (3) Finally, the protein and the polypeptide are mixed with the solution B obtained in step (2), and stirred at 50° C. and 2000 rpm for 1 hour to obtain the skin care composition containing white truffle and plant exosomes.

[0086] Example 4

[0087] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include protein, and the reduced amount thereof is supplemented by the various polypeptide components according to the mass ratio, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0088] Example 5

[0089] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the protein in the skin care composition comprising white truffle and plant exosomes is only recombinant human collagen, and the amount of protein is kept unchanged, and the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0090] Example 6

[0091] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the protein in the skin care composition comprising white truffle and plant exosomes is only hydrolyzed elastin, and the amount of protein is kept unchanged, and the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0092] Example 7

[0093] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include polypeptides, and the reduced amount thereof is supplemented by the protein components according to the mass ratio, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0094] Example 8

[0095] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising white truffle and plant exosomes are carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a mass ratio of 1:1:1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0096] Example 9

[0097] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising white truffle and plant exosomes are carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7 in a mass ratio of 1:1:1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0098] Example 10

[0099] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising white truffle and plant exosomes are carnosine, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 1:1:1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0100] Embodiment 11

[0101] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising white truffle and plant exosomes are carnosine, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 1:1:1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0102] Example 12

[0103] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising white truffle and plant exosomes are dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in a mass ratio of 1:1:1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0104] Example 13

[0105] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising white truffle and plant exosomes is a lactobacillus / soymilk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate and a bifid yeast fermentation product filtrate in a mass ratio of 1.5:0.5:2, and the addition amount of the fermentation product filtrate is kept unchanged, and the other components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0106] Embodiment 14

[0107] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising white truffle and plant exosomes is a lactobacillus / soymilk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate and a yeast / rice fermentation product filtrate in a mass ratio of 1.5:0.5:1.5, and the addition amount of the fermentation product filtrate is kept unchanged, the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0108] Embodiment 15

[0109] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising white truffle and plant exosomes is a lactobacillus / soy milk fermentation product filtrate, a bifid yeast fermentation product filtrate and a yeast / rice fermentation product filtrate in a mass ratio of 1.5:2:1.5, and the addition amount of the fermentation product filtrate is kept unchanged, the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0110] Example 16

[0111] The present embodiment provides a skin care composition comprising white truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising white truffle and plant exosomes is a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, a bifid yeast fermentation product filtrate and a yeast / rice fermentation product filtrate in a mass ratio of 0.5:2:1.5, and the addition amount of the fermentation product filtrate is kept unchanged, the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0112] Comparative Example 1

[0113] This comparative example provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include white truffle extract, and the reduced amount thereof is distributed to Chlorella exosomes, sodium DNA, superoxide dismutase, and fermentation product filtrate in proportion to the number of parts, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0114] Comparative Example 2

[0115] This comparative example provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include Chlorella exosomes, and the reduced amount thereof is distributed to the white truffle extract, sodium DNA, superoxide dismutase, and fermentation product filtrate in proportion, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0116] Comparative Example 3

[0117] This comparative example provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include sodium DNA, and the reduced amount thereof is distributed to the white truffle extract, chlorella exosomes, superoxide dismutase, and fermentation product filtrate in proportion, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0118] Comparative Example 4

[0119] This comparative example provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include superoxide dismutase, and the reduced amount thereof is distributed to the white truffle extract, Chlorella exosomes, DNA sodium, and fermentation product filtrate in proportion, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0120] Comparative Example 5

[0121] This comparative example provides a skin care composition comprising white truffle and plant exosomes, wherein the components of the skin care composition comprising white truffle and plant exosomes do not include fermentation product filtrate, and the reduced amount thereof is distributed to white truffle extract, Chlorella exosomes, DNA sodium, and superoxide dismutase according to the proportion of parts, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0122] Application Example 1

[0123] This application example provides a lotion, the formula of which is as follows, and the dosage unit of each component in the table is "part".

[0124]

[0125]

[0126] Its preparation method is:

[0127] (1) stirring water and a moisturizing agent, heating the mixture to 75° C., and then maintaining the temperature for 1 hour to obtain an aqueous phase liquid;

[0128] (2) stirring the emollient, emulsifier and thickener and heating them to 75° C. for 1 hour to obtain an oil phase liquid;

[0129] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized at 80° C., and after cooling to 35° C., the skin care composition containing white truffle and plant exosomes and essence are added, and the lotion is obtained after homogenization.

[0130] Application Example 2-16

[0131] This application example provides 15 kinds of lotions. The components of the lotions are different from those of Application Example 1 only in that the skin care composition containing white truffles and plant exosomes prepared in Example 1 is replaced by an equal amount of the skin care composition containing white truffles and plant exosomes prepared in Examples 2-16, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0132] Comparative Application Examples 1-5

[0133] This application example provides 5 kinds of lotions. The components of the lotions are different from those of Application Example 1 only in that the skin care composition containing white truffles and plant exosomes prepared in Example 1 is replaced by an equal amount of the skin care composition containing white truffles and plant exosomes prepared in Comparative Examples 1-5, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0134] Test Example 1 Antioxidant Efficacy Study - DPPH Free Radical Scavenging Experiment

[0135] Test samples: compositions provided in Examples 1-16 and Comparative Examples 1-5;

[0136] Test method:

[0137] Accurately weigh 47.7 mg of DPPH reagent, dilute to a 100 ml brown volumetric flask, take 5 ml of it and dilute to a 50 ml brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (47.7 mg / L). Pipette 2 mL of DPPH test solution respectively, add 0.1 mL of the sample provided in Examples 1-16 and Comparative Examples 1-5 as shown in the table below, and then add an appropriate amount of anhydrous ethanol to make the total mass equal. After fully mixing, let stand in the dark for 30 min, use anhydrous ethanol as a blank control, measure its absorbance at 517 nm, and set vitamin C as a control, and calculate the scavenging rate of each extract for DPPH according to the following formula:

[0138] DPPH removal rate (%) = [1-(As-Ar) / A0] × 100%

[0139] Where: As is the absorbance of the extract and DPPH reaction system; Ar is the absorbance of the extract-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0140]

[0141] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the essence can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a highly effective antioxidant recognized by the cosmetics industry. This experiment used vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 1.

[0142] Table 1

[0143]

[0144]

[0145] As can be seen from Table 1, the skin care compositions containing white truffles and plant exosomes in Examples 1-3 have better scavenging effects on DPPH, and the scavenging rates are all above 88%, which is close to that of vitamin C, while the scavenging effects of Examples 4-6 and 7-11 on DPPH are reduced, indicating that the addition of proteins and polypeptides to the composition can make the antioxidant effect of the composition better, and the selection of protein and polypeptide types also affects the antioxidant effect; the selection of the type of fermentation product filtrate in Examples 12-16 also affects the antioxidant effect of the composition; the scavenging effect of the composition in Comparative Examples 1-4 on DPPH is reduced, indicating that the four components of white truffle extract, Chlorella exosomes, DNA sodium, superoxide dismutase, and fermentation product filtrate promote each other in terms of antioxidant effect and have a synergistic effect.

[0146] Test Example 2 Whitening effect - in vitro tyrosinase inhibition test

[0147] Test samples: compositions provided in Examples 1-16 and Comparative Examples 1-5;

[0148] Test method:

[0149] Prepare 5mM L-tyrosine solution (L-tyrosine can be dissolved with a small amount of hydrochloric acid and then fixed to volume with PBS buffer) and tyrosinase solution (diluted to 100U / mL with deionized water). Use a micropipette to transfer 0.4mL of sample, 1mL of 0.05mol / L phosphate buffer (pH=6.8) to the cuvette, then add 0.8mL of 0.5mmol / L tyrosine solution, and finally add 0.40mL of tyrosinase solution, mix quickly, and measure the growth curve of the optical density value with a wavelength of 475nm over time at a constant temperature of 30℃, and obtain the inhibition rate from the slope of the curve. Measure the inhibition rate of extracts with different concentrations respectively, draw a straight line with the concentration as the horizontal axis and the inhibition rate as the vertical axis, and after obtaining the linear equation, calculate the extract concentration when the inhibition rate is 50%, which is IC50. The results are shown in Table 2:

[0150] Table 2

[0151]

[0152]

[0153] As shown in Table 2, the skin care composition comprising white truffle and plant exosomes of the present invention has a good inhibitory effect on tyrosinase. Among them, the IC50 values ​​of Examples 1-3 are lower than those of Examples 4-11, indicating that the addition of proteins and polypeptides in the composition can better improve the whitening effect; as shown in Examples 12-16, the type of fermentation product filtrate selection also significantly affects the efficacy of the final product; the IC50 value of Example 1 is lower than that of Comparative Examples 1-5, indicating the synergistic effect of white truffle extract, Chlorella exosomes, DNA sodium, superoxide dismutase, and fermentation product filtrate in the composition of the present invention, verifying the rationality of the composition.

[0154] Test Example 3: Human evaluation test:

[0155] 126 volunteers aged 20-45 were selected and randomly divided into 21 groups, 6 people in each group, half male and half female. The 21 groups of volunteers used the lotions prepared in Application Examples 1-16 and Comparative Application Examples 1-5 on their left sides, once a day in the morning and evening, 5±0.5g of the product was applied to the face each time, and massaged for 2 minutes, and continued to use for 30 days. The volunteers scored the skin whitening ability and skin elasticity of the left and right faces, with the highest score being 5 points and the lowest score being 1 point. The evaluation results are shown in Table 3 (average score):

[0156] Table 3

[0157]

[0158]

[0159] As shown in Table 3, the composition of the present invention can be added into cosmetic products to significantly improve the sensory scores of skin whitening and elasticity.

[0160] Test Example 4: Safety Evaluation Experiment

[0161] This project evaluates irritation through the red blood cell hemolysis test. The red blood cell hemolysis test is one of the alternative methods to the rabbit eye irritation test (Draize test). The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolution and the degree of denaturation. Internationally, the RBC test is mainly used to evaluate the eye irritation of chemicals such as cosmetics and raw materials. The classification standards are shown in the following table.

[0162]

[0163] The results of the red blood cell hemolysis experiment on the samples of Examples 1-16 are shown in Table 4, where L / D is the ratio of HD50 (the sample concentration when 50% of the red blood cells are hemolyzed) to DI (protein denaturation index).

[0164] Table 4

[0165]

[0166]

[0167] It can be seen from Table 4 that the skin care composition comprising white truffle and plant exosomes of the present invention is mild and non-irritating, and has high safety in use.

[0168] The applicant declares that the present invention illustrates a skin care composition containing white truffles and plant exosomes and its application through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0169] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0170] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care composition comprising white truffle and plant exosomes, characterized in that: The skin care composition comprising white truffle and plant exosomes comprises white truffle extract, Chlorella exosomes, DNA sodium, superoxide dismutase and fermentation product filtrate; The fermentation product filtrate includes any one of lactobacillus / soymilk fermentation product filtrate, lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, bifid yeast fermentation product filtrate or yeast / rice fermentation product filtrate or a combination of at least two thereof.

2. The skin care composition comprising white truffle and plant exosomes according to claim 1, characterized in that The skin care composition containing white truffle and plant exosomes comprises, by weight, 0.5-2 parts of white truffle extract, 1-3 parts of chlorella exosomes, 1-2 parts of sodium DNA, 3-5 parts of superoxide dismutase and 5-10 parts of fermentation product filtrate.

3. The skin care composition comprising white truffle and plant exosomes according to claim 1 or 2, characterized in that: The fermentation product filtrate is a combination of a lactobacillus / soy milk fermentation product filtrate, a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, a bifida yeast fermentation product filtrate, and a yeast / rice fermentation product filtrate; Preferably, the mass ratio of the lactobacillus / soymilk fermentation product filtrate, the lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is (1-2):(0.1-1):(1-3):(1-2).

4. The skin care composition comprising white truffle and plant exosomes according to any one of claims 1 to 3, characterized in that: The skin care composition comprising white truffle and plant exosomes also includes protein; Preferably, the skin care composition comprising white truffle and plant exosomes further comprises 1-4 parts of protein by weight.

5. The skin care composition comprising white truffle and plant exosomes according to claim 4, characterized in that The protein includes recombinant human collagen and / or hydrolyzed elastin.

6. The skin care composition comprising white truffle and plant exosomes according to claim 5, characterized in that The proteins include recombinant human collagen and hydrolyzed elastin; Preferably, the mass ratio of the recombinant human collagen to the hydrolyzed elastin is 1:(0.5-2).

7. The skin care composition comprising white truffle and plant exosomes according to any one of claims 1 to 6, characterized in that: The skin care composition comprising white truffle and plant exosomes also includes a polypeptide; Preferably, the skin care composition comprising white truffle and plant exosomes further comprises 1-5 parts of polypeptides by weight.

8. The skin care composition comprising white truffle and plant exosomes according to claim 7, characterized in that The polypeptide comprises any one of carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 or palmitoyl tetrapeptide-7, or a combination of at least two thereof.

9. The skin care composition comprising white truffle and plant exosomes according to claim 8, characterized in that The polypeptides include carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7; Preferably, the mass ratio of carnosine, dipeptide diaminobutyryl benzylamide diacetate, acetyl hexapeptide-8, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 is (2-4):(0.5-1.5):(1-2):(1-2):(1-2).

10. Use of the skin care composition comprising white truffle and plant exosomes according to any one of claims 1 to 9 in the preparation of cosmetics.

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