A matrix mixing device and method for high-density large-scale adherent cell culture

Abstract

The present invention discloses a matrix mixing device and method for high-density large-scale adherent cell culture, which relates to the technical field of mixing devices, including a base, a mounting frame and a mixing cylinder fixedly connected to the inner side of the mounting frame; the mixing cylinder includes an outer cylinder connected to the mounting frame and an inner cylinder rotatably connected to the outer cylinder, and a partition is provided in the middle of the feeding hopper, and the partition divides the feeding hopper into two chambers, one of which is used to put powder raw materials, and the other chamber is used to put magnetic powder. The present invention is provided with a partition plate, and when the cover plate is covered on the inner cylinder, the inner cylinder can be equally divided into a number of independent chambers. After the separation, the solution in each chamber is small in volume, and it is easier to form turbulence when stirring, which is conducive to the uniform dispersion of the powder; and two chambers are provided on the feeding hopper to respectively put in water-insoluble magnetic powder and powder, and after mixing, they are put into the mixing cylinder for mixing, so that a certain distance is maintained between the powder particles, thereby reducing the occurrence of clumping.

Description

Technical Field

[0001] The invention relates to the technical field of mixing devices, and in particular to a matrix mixing device and method for high-density large-scale adherent cell culture. Background Art

[0002] Cell culture medium not only provides the nutrients required by cells and promotes cell growth and reproduction, but also creates a good living environment for cells. In the prior art, when preparing culture medium, first take powder (including inorganic salts, amino acids, vitamins, carbon sources and additives) and mix them with water in a certain ratio. After sufficient mixing, the pH value is tested, and buffer (acidic or alkaline solution) is added according to the pH value until the pH value of the solution reaches a value suitable for cell growth. For example, the pH value of the culture medium for adherent cells is usually 7.2-7.4. After filtering and removing impurities and sterilizing at high temperature and high pressure, it can be stored or used.

[0003] In cell factories and some biopharmaceutical sites, large-scale cell culture is usually required. What is different from the general laboratory preparation process is that the large-scale cell culture medium preparation process involves the mixing of a large amount of raw materials. The basal liquid needs to mix a large amount of powder with a certain proportion of water. Since the culture medium powder contains some adhesive components, such as certain amino acids, vitamins or inorganic salts, these components adhere to each other due to surface tension when mixed with water, forming clumps. Different components in the culture medium have different solubilities. When these components are dissolved in water, components with lower solubility may more easily form precipitation or clumps. The above situation will be more serious in the large-scale mixing preparation process. Since there are more mixed materials, the fluidity during the mixing process is relatively worse, it is more difficult to form turbulence during stirring, and it is more difficult to stir the powder evenly, so it is more likely to produce clumping.

[0004] In the prior art, a Chinese patent for a raw material mixing device for cell culture medium (publication number CN112827396B) is proposed to solve the above-mentioned technical problems. The technical solution disclosed in the patent document is as follows: "The raw material mixing device for cell culture medium is provided with multiple groups of partition plates on the surface of the driving roller. The partition plates cooperate with the sleeve plate to separate the inside of the mixing cylinder. The raw materials can be placed separately between two adjacent partition plates, that is, the materials in each partition chamber can be different. In this way, the materials are turned over in the mixing cylinder to achieve material mixing, so that multiple groups of materials are mixed in the mixing cylinder at the same time." In this solution, the mixing environment can be divided to form multiple independent partition chambers. In this solution, the mixing environment can be divided into many small chambers, avoiding the situation of powder clumping. Although it can reduce the clumping to a certain extent, this method is not suitable for the operation of mixing powder with water to prepare liquid culture medium. This technology can only rely on the mixing cylinder to mix the raw materials, and the powder will produce a clumping phenomenon at the moment of adding water. Once the clumping phenomenon occurs, it is difficult to eliminate it only by turning it over, so the occurrence of powder clumping cannot be eliminated. Summary of the invention

[0005] The object of the present invention is to provide a matrix mixing device and method for high-density large-scale adherent cell culture, so as to solve the problem that in the prior art, during the preparation of cell culture medium, powder and water are easily clumped during mixing, resulting in insufficient mixing.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] The invention provides a matrix mixing device for high-density large-scale adherent cell culture, comprising a base, a mounting frame and a mixing barrel fixedly connected to the inner side of the mounting frame; the mixing barrel comprises an outer barrel connected to the mounting frame and an inner barrel rotatably connected to the outer barrel, a side wall of the inner barrel is provided with a plurality of feeding grooves, a connecting frame is fixedly connected to the side wall of the outer barrel, a feeding hopper is fixedly connected to the side of the connecting frame away from the outer barrel, a partition is arranged in the middle of the feeding hopper, the partition divides the feeding hopper into two chambers, one of which is used for feeding powder raw materials, and the other is used for feeding magnetic powder, and a through groove is arranged on the side of the outer barrel close to the connecting frame; a quantitative premixing component is arranged between the feeding hopper and the mixing barrel, the quantitative premixing component is used for taking a certain amount of magnetic powder and powder raw materials for premixing, and then feeding them into the mixing barrel; hydraulic cylinders are symmetrically fixedly connected to the opposite sides of the mounting frame, and two hydraulic cylinders are provided. A push frame is fixedly connected between the output ends of the cylinders, and a cover plate is rotatably connected to the middle part of the push frame. The cover plate is used to cover one end of the inner cylinder, and a sealing ring is arranged on the side of the cover plate away from the push frame; a plurality of partition plates are fixedly connected to the side of the cover plate away from the push frame, and the partition plate divides the end face of the cover plate into a number of sectors of equal size, a transmission shaft is fixedly connected to one side of the inner wall of the inner cylinder, the transmission shaft is a hollow structure, and a plurality of slots matching the shape of the partition plates are opened on the side wall, a plurality of insertion rails are fixedly connected to the inner side of the inner cylinder at equal intervals, and the internal shape of the insertion rails matches the shape of the end of the partition plates, a sealing gasket is fixedly connected to the side of the inner wall of the inner cylinder close to the transmission shaft, and the transmission shaft and the partition plate are used in conjunction with each other by plugging; a discharge pipe is arranged on the side of the outer cylinder away from the connecting frame, and an electric valve is installed on the discharge pipe; a mixing motor is fixedly installed on the top of the base, and the mixing motor is used to drive the cover plate and the inner cylinder to flip.

[0008] By adopting the above technical solution, by setting a partition plate, the inner cylinder can be equally divided into several independent chambers when the cover plate is closed on the inner cylinder. After separation, the solution in each chamber is smaller in volume and is more likely to form turbulence during stirring, which is conducive to the uniform dispersion of the powder. Based on the above design, the powder to be put in is improved by setting two chambers on the feeding hopper to respectively put in water-insoluble magnetic powder and powder, and after mixing, they are put into the mixing cylinder for mixing. Since the powder is "diluted" by adding the magnetic powder, a certain distance is maintained between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of clumping.

[0009] A further improvement of the technical solution of the present invention is that: a support frame is fixedly connected to the top of the base, a flip shaft is rotatably connected between the inner sides of the support frame, the mounting frame is fixedly connected to the outer side of the flip shaft, a flip motor is fixedly installed on the top of the base, one end of the flip shaft extends to the other side of the support frame and is fixedly connected to the output end of the flip motor; a reducer is fixedly installed on the top of the base, one end of the reducer is connected to the output end of the mixing motor, the other end of the reducer is fixedly connected to the main shaft, a key slot is provided at the central position of the end of the main shaft, one end of the central axis of the cover plate extends to the other side of the push frame and is fixedly connected with a transmission key, the transmission key and the key slot shape match each other and the two are used in combination, since the mixing barrel needs to be flipped around the flip shaft and needs to rotate around the main shaft when mixing, in order to ensure that the two can be disengaged and transmission can be quickly realized, a key slot and a transmission key are provided, the two can be quickly disengaged, and can rotate synchronously when the transmission key is inserted into the key slot (when the two are coaxial).

[0010] By adopting the above technical solution, the mounting frame is installed on the flip shaft so that the mixing drum can be flipped along with the flip shaft. When mixing materials, the mixing drum is flipped to a vertical state so that the mixed liquid can be churned along with the flipping of the mixing drum. When adding materials, the mixing drum is flipped to a horizontal state so that the side of the mixing drum with the opening faces upward, and the feeding hopper is in a lower position, which is convenient for adding powder and magnetic powder.

[0011] A further improvement of the technical solution of the present invention is that: the quantitative premixing component includes a valve body, a premixing chamber is fixedly connected to the bottom of the valve body, the valve body is communicated with the through groove through the premixing chamber, a stirring rod is rotatably connected between the inner walls of the premixing chamber, a premixing motor is fixedly connected to the outer wall of the premixing chamber, and the output end of the premixing motor extends to the interior of the premixing chamber and is fixedly connected to one end of the stirring rod; two material guide pipes are symmetrically arranged on the top of the valve body, the top of the material guide pipe is communicated with the feeding hopper, a valve core is rotatably connected between the inner walls of the valve body, two valve cavities are provided on the valve core, an electric actuator is fixedly connected to one side of the valve body, and the output end of the electric actuator is fixedly connected to the central axis of the valve core; a baffle is rotatably connected between the inner walls of the premixing chamber through a torsion spring, a limit block for limiting the baffle is fixedly connected to the bottom end of the inner wall of the premixing chamber, and the baffle is used in conjunction with the feeding trough.

[0012] By adopting the above technical solution, the electric actuator is controlled to drive the valve core to rotate. When the valve core rotates to the point where the valve cavity is facing upward, the powder and magnetic powder in the feed hopper begin to enter the valve cavity through the guide pipe. As the valve core continues to rotate, when the valve cavity faces downward and there is an area connected to the premixing chamber, the powder and magnetic powder will fall into the premixing chamber. By controlling the mixing motor to rotate the stirring rod, the powder and magnetic powder are simply premixed.

[0013] A further improvement of the technical solution of the present invention is that fixed disks are provided on both sides of the mixing barrel, one of the fixed disks is fixedly connected to one side of the push frame, and the other fixed disk is fixedly connected between the inner sides of the mounting frames, a plurality of mixing magnets are fixedly connected between the inner walls of the fixed disks, the mixing magnets are arranged in a ring shape and the curvatures between two adjacent mixing magnets are equal, isolation plates are provided between adjacent mixing magnets, the mixing magnets on different fixed disks correspond to each other one by one and have opposite facing magnetic poles, and the adjacent mixing magnets on the same fixed disk have opposite magnetic poles on the same side.

[0014] By adopting the above technical solution, magnetic fields are set on both sides of the mixing cylinder. After adding materials, the magnetic powder is mixed in the solution. The solution and the magnetic powder rotate around the central axis of the inner cylinder as the inner cylinder rotates. During the process, the magnetic field composed of several groups of mixing magnets causes the magnetic powder to reciprocate in the axial direction of the inner cylinder at the same time, so that the powder and the solution are fully mixed.

[0015] A further improvement of the technical solution of the present invention is that: the discharge pipe is in an S-shaped structure, a filter screen is detachably connected to the inside of the discharge pipe, a nozzle is fixedly connected to one side of the discharge pipe, a thread is provided on the outside of the nozzle and a collecting barrel is connected to the thread, and a sealing ring is provided between the collecting barrel and the nozzle; an end cover is connected to the side of the collecting barrel away from the nozzle through a thread, a collecting magnet is fixedly connected to one side of the inner wall of the end cover, the collecting magnet is used to absorb and collect magnetic powder in the discharge, and a guide barrel is fixedly connected to the side of the inner wall of the collecting barrel close to the nozzle.

[0016] By adopting the above technical solution, a collecting cylinder is arranged above the filter screen side, and when the electric valve is opened and the feeding trough located at the lower part is connected with the discharging pipe, the solvent can flow out into the container through the feeding trough and the discharging pipe. During the process, the magnetic powder and other solid impurities with larger particles are blocked on the filter screen and sucked into the collecting cylinder by the collecting magnet. The magnetic powder adsorbed on the magnet is also magnetic, so when the collecting magnet adsorbs more magnetic powder, it will not affect the subsequent adsorption effect.

[0017] A further improvement of the technical solution of the present invention is that a plurality of shaking rods are fixedly connected to one side of the cover plate close to the partition plate 62, and the shaking rods cooperate with the baffle plate to make the baffle plate shake during the movement.

[0018] By adopting the above technical solution, during the rotation of the inner cylinder, the baffle is shaken by the shaking rod. Specifically, when one side of the baffle contacts the shaking rod, the baffle will flip upward at a certain arc until it continues to rotate until the baffle is separated from the shaking rod. At this time, the baffle rotates downward again under the action of the torsion spring. There are multiple shaking rods, which make the baffle show a shaking motion, thereby assisting the powder and magnetic powder to fall into the mixing cylinder.

[0019] A further improvement of the technical solution of the present invention is that an oblique opening is provided at a portion near one side of the top of the feeding hopper, a slide rail is symmetrically fixedly connected to the side wall of the feeding hopper near the oblique opening, and a slide cover is slidably connected between the two slide rails.

[0020] By adopting the above technical solution, there is an inclined opening near one side of the top of the feeding hopper for feeding, and the angle of the wedge is 45°. When the feeding hopper is flipped to a horizontal state, the sliding cover slides downward and automatically opens under the action of gravity (it can be opened manually when stuck in special circumstances) to facilitate feeding. When flipped to a vertical state, the sliding cover will slide to cover the inclined opening to reduce the contamination of the powder by microorganisms in the air.

[0021] A further improvement of the technical solution of the present invention is that two auxiliary legs are symmetrically fixedly connected to one side of the mounting frame, and a reinforcing plate is fixedly connected between the auxiliary legs and the mounting frame.

[0022] By adopting the above technical solution, auxiliary legs are provided to provide auxiliary support when the mixing drum is in a horizontal state, so as to reduce the pressure on the flip shaft.

[0023] The present invention also provides a matrix mixing method for high-density large-scale adherent cell culture, comprising the following steps:

[0024] S1: Measure a certain amount of culture medium powder and distilled water, divide the measured distilled water into several equal portions and add them separately into the mixing cylinder;

[0025] The solution is divided into multiple independent mixing chambers. Since the solution in each mixing chamber has a smaller volume, it is easier to form turbulence during stirring, which helps to evenly disperse the powder.

[0026] S2: Measure 10% of the amount of magnetic powder taken from the culture medium powder in step S1, mix the magnetic powder and the culture medium powder according to the above ratio, and add them to the mixing drum in multiple times, and on average, add the mixture of powder and magnetic powder at least 5 times for each portion of distilled water;

[0027] By first adding water-insoluble magnetic powder into the powder and mixing it, and then adding it into each mixing chamber in batches, the powder is "diluted" by adding the magnetic powder, and the magnetic powder is insoluble in water, it can act as a "carrier" or "bridge" to keep a certain distance between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of clumping.

[0028] S3: applying a magnetic field to the mixing drum, wherein the magnetic field is arranged in a ring shape at a plurality of points on the circumference of the mixing drum, and the magnetic fields at adjacent points have opposite directions, and the mixing drum is controlled to flip, so that the magnetic powder flips in the circumferential direction in the mixed liquid and reciprocates in the axial direction at the same time, until the culture medium powder is completely dissolved in the distilled water;

[0029] Magnetic fields are set on both sides of the mixing cylinder. After adding materials, the magnetic powder is mixed in the solution. The solution and the magnetic powder rotate around the central axis along with the rotation of the inner cylinder. During the process, the magnetic field composed of several groups of mixing magnets makes the magnetic powder reciprocate in the axial direction of the inner cylinder at the same time, so that the powder and the solution are fully mixed.

[0030] S4: Filter the mixed liquid until the magnetic powder and impurities in the mixed liquid are filtered out.

[0031] Due to the adoption of the above technical solution, the present invention has the following technical advances compared with the prior art:

[0032] 1. The present invention provides a partition plate, and when the cover plate is closed on the inner cylinder, the inner cylinder can be equally divided into several independent chambers. After the separation, the solution in each chamber is small in volume, so it is easier to form turbulence during stirring, which is conducive to the uniform dispersion of the powder. Based on the above design, the powder to be put in is improved by providing two chambers on the feeding hopper to respectively put in water-insoluble magnetic powder and powder, and after mixing, they are put into the mixing cylinder for mixing. Since the powder is "diluted" by adding the magnetic powder, a certain distance is maintained between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of clumping.

[0033] 2. The present invention installs the mounting frame on the flip shaft so that the mixing drum can flip along with the flip shaft. When mixing materials, the mixing drum is flipped to a vertical state so that the mixed liquid can tumble along with the flipping of the mixing drum. When adding materials, the mixing drum is flipped to a horizontal state so that the side of the mixing drum with the opening faces upward, and the feeding hopper is in a lower position, which is convenient for adding powder and magnetic powder.

[0034] 3. The present invention sets magnetic fields on both sides of the mixing cylinder. After adding materials, the magnetic powder is mixed in the solution. The solution and the magnetic powder rotate around the central axis of the inner cylinder as the inner cylinder rotates. During the process, the magnetic field composed of several groups of mixing magnets causes the magnetic powder to reciprocate in the axial direction of the inner cylinder at the same time, so that the powder and the solution are fully mixed.

[0035] 4. The present invention arranges a collecting cylinder above the filter screen, opens the electric valve and when the feeding trough at the lower part is connected with the discharging pipe, the solvent can flow out into the container through the feeding trough and the discharging pipe. During the process, the magnetic powder and other solid impurities with larger particles are blocked on the filter screen and sucked into the collecting cylinder by the collecting magnet.

[0036] 5. The present invention arranges a plurality of shaking rods on one side of the cover plate close to the partition plate. The shaking rods cooperate with the baffle to make the baffle shake during the movement. Specifically, during the rotation of the inner cylinder, the baffle is shaken by the shaking rods. When one side of the baffle contacts the shaking rods, the baffle is flipped upward by a certain arc until it continues to rotate until the baffle is separated from the shaking rods. At this time, the baffle rotates downward again under the action of the torsion spring. A plurality of shaking rods are arranged, so that the baffle exhibits a shaking action, thereby assisting the powder and magnetic powder to fall into the mixing cylinder, thereby avoiding material jamming. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The present invention will be further described below in conjunction with the accompanying drawings.

[0038] Figure 1 This is one of the structural schematic diagrams of the mixing drum of the present invention when it is in a horizontal state and the cover plate is opened;

[0039] Figure 2 The second structural schematic diagram of the mixing drum of the present invention when it is in a horizontal state and the cover plate is opened;

[0040] Figure 3 It is a schematic diagram of the structure of the mixing drum of the present invention when it is in a vertical state and the cover plate is closed;

[0041] Figure 4 It is a schematic diagram of the local structure when the mixing drum of the present invention is in a horizontal state and the cover plate is opened;

[0042] Figure 5 It is a schematic diagram of the structure of the cover plate and the partition plate of the present invention;

[0043] Figure 6 This is one of the schematic diagrams of the split structure of the mixing cylinder of the present invention;

[0044] Figure 7 This is the second schematic diagram of the split structure of the mixing cylinder of the present invention;

[0045] Figure 8 It is a schematic cross-sectional structural diagram of the mixing barrel of the present invention;

[0046] Fig. 9 It is a schematic cross-sectional view of the discharge pipe and the collecting tube of the present invention;

[0047] Fig.10 This is a schematic diagram of the structure of the mixed material magnet arrangement of the present invention;

[0048] Fig.11 For the present invention Figure 1 Enlarged view of point A in the middle;

[0049] Fig.12 For the present invention Figure 1 Enlarged view of point B in the middle.

[0050] In the figure: 1, base; 2, support frame; 3, mounting frame; 4, mixing cylinder; 41, outer cylinder; 42, inner cylinder; 43, transmission shaft; 44, feeding trough; 45, insertion rail; 46, sealing gasket; 47, insertion slot; 51, connecting frame; 52, feeding hopper; 53, valve body; 54, valve core; 55, valve cavity; 56, material guide pipe; 57, electric actuator; 58, premixing chamber; 59, stirring rod; 510, baffle; 511, premixing motor; 512, limit block; 61, cover plate; 62, partition Plate; 63, hydraulic cylinder; 64, push frame; 71, flip motor; 72, flip shaft; 73, mixing motor; 74, reducer; 75, main shaft; 76, transmission key; 77, keyway; 81, collecting cylinder; 82, nozzle; 83, end cover; 84, collecting magnet; 85, guide cylinder; 86, filter screen; 87, electric valve; 91, fixed plate; 92, mixing magnet; 93, isolation plate; 11, discharge pipe; 12, slide rail; 13, slide cover; 14, shaking rod; 15, auxiliary support leg. DETAILED DESCRIPTION

[0051] The present invention is further described in detail below in conjunction with embodiments:

[0052] Example 1

[0053] like Figure 1 , Figure 2 and Figure 3As shown, the present invention provides a matrix mixing device for high-density large-scale adherent cell culture, comprising a base 1, a mounting frame 3 and a mixing barrel 4 fixedly connected to the inner side of the mounting frame 3; the mixing barrel 4 comprises an outer barrel 41 connected to the mounting frame 3 and an inner barrel 42 rotatably connected to the outer barrel 41, a side wall of the inner barrel 42 is provided with a plurality of feeding grooves 44, a connecting frame 51 is fixedly connected to the side wall of the outer barrel 41, a feeding hopper 52 is fixedly connected to the side of the connecting frame 51 away from the outer barrel 41, a partition is provided in the middle of the feeding hopper 52, the partition divides the feeding hopper 52 into two chambers, one of which is used for feeding powder raw materials, and the other is used for feeding magnetic powder, and a through groove is provided on the side of the outer barrel 41 close to the connecting frame 51; a quantitative premixing component is provided between the feeding hopper 52 and the mixing barrel 4, the quantitative premixing component is used to take a certain amount of magnetic powder and powder raw materials for premixing, and then feed them into the mixing barrel 4; hydraulic cylinders 63 are symmetrically fixedly connected to the opposite sides of the mounting frame 3, and a The push frame 64 has a cover plate 61 rotatably connected to the middle of the push frame 64. The cover plate 61 is used to cover one end of the inner cylinder 42. A sealing ring is provided on the side of the cover plate 61 away from the push frame 64. A plurality of partition plates 62 are fixedly connected to the side of the cover plate 61 away from the push frame 64. The partition plates 62 divide the end surface of the cover plate 61 into a plurality of sectors of equal size. A transmission shaft 43 is fixedly connected to one side of the inner wall of the inner cylinder 42. The transmission shaft 43 is a hollow structure and a plurality of slots 47 matching the shape of the partition plates 62 are opened on the side wall. A number of rails 45 are fixedly connected to the inner side of the cylinder 42 at equal intervals, and the internal shape of the rails 45 matches the shape of the end of the partition plate 62. A sealing gasket 46 is fixedly connected to the inner wall of the inner cylinder 42 on the side close to the transmission shaft 43, and the transmission shaft 43 and the partition plate 62 are used in conjunction with each other by plugging. A discharge pipe 11 is provided on the side of the outer cylinder 41 away from the connecting frame 51, and an electric valve 87 is installed on the discharge pipe 11. A mixing motor 73 is fixedly installed on the top of the base 1, and the mixing motor 73 is used to drive the cover plate 61 and the inner cylinder 42 to flip.

[0054] In this embodiment, by providing a partition plate 62, when the cover plate 61 is covered on the inner cylinder 42, the inner cylinder 42 can be equally divided into a number of independent chambers. After the separation, the solution in each chamber is smaller in volume and is more likely to form turbulence during stirring, which is conducive to the uniform dispersion of the powder. Based on the above design, the powder to be put in is improved by providing two chambers on the feeding hopper 52 to respectively put in the water-insoluble magnetic powder and powder, and after mixing, they are put into the mixing cylinder 4 for mixing. Since the powder is "diluted" by adding the magnetic powder, and the magnetic powder is insoluble in water, it can act as a "carrier" or "bridge" to keep a certain distance between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of clumping.

[0055] Example 2

[0056] like Figure 1 , Figure 2and Figure 3 As shown, on the basis of Example 1, the present invention provides a technical solution: preferably, the top of the base 1 is fixedly connected with a support frame 2, the inner side of the support frame 2 is rotatably connected with a flip shaft 72, the mounting frame 3 is fixedly connected to the outer side of the flip shaft 72, and a flip motor 71 is fixedly installed on the top of the base 1, one end of the flip shaft 72 extends to the other side of the support frame 2 and is fixedly connected to the output end of the flip motor 71; a reducer 74 is fixedly installed on the top of the base 1, one end of the reducer 74 is connected to the output end of the mixing motor 73, and the other end of the reducer 74 A main shaft 75 is fixedly connected, and a key slot 77 is provided at the central position of the end of the main shaft 75. One end of the central axis of the cover plate 61 extends to the other side of the push frame 64 and is fixedly connected with a transmission key 76. The transmission key 76 and the key slot 77 match in shape and the two are used in conjunction with each other. Since the mixing barrel 4 needs to be flipped around the flip axis 72 and needs to rotate around the main shaft 75 when mixing, in order to ensure that the two can be disengaged and transmission can be achieved quickly, a key slot 77 and a transmission key 76 are provided. The two can be quickly disengaged and can rotate synchronously when the transmission key 76 is inserted into the key slot 77 (when the two are coaxial).

[0057] In order to facilitate the batch feeding of powder and improve the mixing effect, the mixing of raw materials is achieved by a rotation method similar to the Ferris wheel. Correspondingly, the feeding hopper 52 is arranged above the mixing barrel 4, so it is inconvenient to feed the materials.

[0058] In this embodiment, the mounting frame 3 is installed on the flip shaft 72 so that the mixing drum 4 can be flipped along with the flip shaft 72. When mixing materials, the mixing drum 4 is flipped to a vertical state so that the mixed liquid can be churned along with the flipping of the mixing drum 4. When adding materials, the mixing drum 4 is flipped to a horizontal state so that the side of the mixing drum 4 with the opening faces upward, and the feeding hopper 52 is in a lower position, which is convenient for adding powder and magnetic powder.

[0059] Example 3

[0060] like Figure 1 , Figure 8 and Fig.11As shown, on the basis of Example 2, the present invention provides a technical solution: preferably, the quantitative premixing component includes a valve body 53, the bottom of the valve body 53 is fixedly connected to a premixing chamber 58, the valve body 53 is connected to the through groove through the premixing chamber 58, a stirring rod 59 is rotatably connected between the inner walls of the premixing chamber 58, the outer wall of the premixing chamber 58 is fixedly connected to a premixing motor 511, the output end of the premixing motor 511 extends to the interior of the premixing chamber 58 and is fixedly connected to one end of the stirring rod 59; two material guide pipes are symmetrically arranged on the top of the valve body 53 56, the top of the guide pipe 56 is communicated with the feeding hopper 52, a valve core 54 is rotatably connected between the inner walls of the valve body 53, two valve chambers 55 are opened on the valve core 54, an electric actuator 57 is fixedly connected to one side of the valve body 53, and the output end of the electric actuator 57 is fixedly connected to the central axis of the valve core 54; a baffle 510 is rotatably connected between the inner walls of the premixing chamber 58 through a torsion spring, and a limit block 512 for limiting the baffle 510 is fixedly connected to the bottom end of the inner wall of the premixing chamber 58, and the baffle 510 is used in conjunction with the feeding trough 44.

[0061] In this embodiment, the valve core 54 is driven to rotate by controlling the operation of the electric actuator 57. When the valve core 54 rotates until the valve cavity 55 is upward, the powder and magnetic powder in the hopper 52 begin to enter the valve cavity 55 through the conducting pipe. The volume between the valve cavity 55 and the valve body 53 facing the magnetic powder is 10% of the volume between the valve cavity 55 and the valve body 53 facing the powder. Each time the valve core 54 rotates until the valve cavity 55 is filled, the magnetic powder taken is 10% of the powder. As the valve core 54 continues to rotate, when the valve cavity 55 faces downward and there is an area connected with the premixing chamber 58, the magnetic powder behind the powder will fall into the premixing chamber 58, and the mixing motor 73 is controlled to work to rotate the stirring rod 59 to simply premix the powder and the magnetic powder.

[0062] Example 4

[0063] like Figure 6 , Figure 7 and Fig.10 As shown, on the basis of Example 3, the present invention provides a technical solution: preferably, fixed disks 91 are provided on both sides of the mixing barrel 4, wherein one fixed disk 91 is fixedly connected to one side of the push frame 64, and the other fixed disk 91 is fixedly connected between the inner sides of the mounting frame 3, and a plurality of mixing magnets 92 are fixedly connected between the inner walls of the fixed disks 91, the mixing magnets 92 are arranged in a ring shape, and the curvatures of two adjacent mixing magnets 92 are equal, and isolation plates 93 are provided between adjacent mixing magnets 92, the mixing magnets 92 on different fixed disks 91 correspond to each other one by one and have opposite facing magnetic poles, and the adjacent mixing magnets 92 on the same fixed disk 91 have opposite magnetic poles on the same side.

[0064] Since the mixing barrel 4 can only rely on the gravity of the mixed liquid to stir it during the turning process, and this force is applied from the outside, it is not easy to stir up the clumped raw materials. Therefore, it is still difficult to achieve an ideal mixing effect by only relying on the turning of the mixing barrel 4.

[0065] In this embodiment, magnetic fields are set on both sides of the mixing cylinder 4. After adding materials, the magnetic powder is mixed in the solution. The solution and the magnetic powder rotate around the central axis of the inner cylinder 42 as the inner cylinder 42 rotates. During the process, the magnetic field formed by several groups of mixing magnets 92 causes the magnetic powder to reciprocate in the axial direction of the inner cylinder 42 at the same time, so that the powder and the solution are fully mixed.

[0066] The fixing plate 91 is not in contact with the mixing cylinder 4 and the cover plate 61;

[0067] like Fig.10 As shown, it is a schematic diagram of the arrangement of the mixing magnets 92 arranged on both sides of the mixing barrel 4, wherein N represents the side of the mixing magnet 92 close to the mixing barrel 4 is the magnetic pole N, and S represents the side close to the mixing barrel 4 is the magnetic pole S.

[0068] Example 5

[0069] like Figure 4 , Fig. 9 and Fig.10 As shown, on the basis of Example 4, the present invention provides a technical solution: preferably, the discharge pipe 11 is in an S-shaped structure, the inside of the discharge pipe 11 is detachably connected with a filter screen 86, one side of the discharge pipe 11 is fixedly connected with a nozzle 82, the outer side of the nozzle 82 is provided with a thread and is connected with a collecting barrel 81 through the thread, and a sealing ring is provided between the collecting barrel 81 and the nozzle 82; the side of the collecting barrel 81 away from the nozzle 82 is connected with an end cover 83 through a thread, one side of the inner wall of the end cover 83 is fixedly connected with a collecting magnet 84, the collecting magnet 84 is used to absorb and collect magnetic powder in the discharge, and the side of the inner wall of the collecting barrel 81 close to the nozzle 82 is fixedly connected with a guide barrel 85.

[0070] Since magnetic particles are mixed into the culture medium solvent, they need to be removed by filtration after mixing;

[0071] In this embodiment, a collecting cylinder 81 is arranged above the filter screen 86. When the electric valve 87 is opened and the feeding trough 44 at the lower part is connected with the discharging pipe 11, the solvent can flow out into the container through the feeding trough 44 and the discharging pipe 11. During the process, the magnetic powder and other solid impurities with larger particles are blocked on the filter screen 86 and sucked into the collecting cylinder 81 by the collecting magnet 84. The magnetic powder adsorbed on the magnet is also magnetic, so when the collecting magnet 84 adsorbs more magnetic powder, it will not affect the subsequent adsorption effect.

[0072] It should be noted that, since impurities also need to be filtered out during the culture medium preparation process, the provision of the filter 86 does not increase the complexity of the process, but only increases the adsorption process of magnetic powder during the filtration process, which does not affect the normal process flow.

[0073] Preferably, the mesh size of the filter 86 is 0.22 μm, and the diameter of the magnetic powder particles is 0.1 mm.

[0074] like Figure 7 and Fig.10 As shown, preferably, a plurality of shaking rods 14 are fixedly connected to one side of the cover plate 61 close to the partition plate 62, and the shaking rods 14 cooperate with the baffle plate 510 to make the baffle plate 510 shake during the movement.

[0075] In this embodiment, during the rotation of the inner cylinder 42, the baffle 510 is shaken by the shaking rod 14. Specifically, when one side of the baffle 510 contacts the shaking rod 14, the baffle 510 will flip upward at a certain arc until it continues to rotate until the baffle 510 is separated from the shaking rod 14. At this time, the baffle 510 rotates downward again under the action of the torsion spring. There are multiple shaking rods 14, so that the baffle 510 exhibits a shaking motion.

[0076] like Figure 1 , Figure 4 and Fig.11 As shown, preferably, a bevel is provided at a top portion of the feeding hopper 52 near one side, and a slide rail 12 is symmetrically fixedly connected to the side wall of the feeding hopper 52 near the bevel, and a slide cover 13 is slidably connected between the two slide rails 12.

[0077] In this embodiment, there is an inclined opening near one side of the top of the feeding hopper 52 for feeding, and the angle of the wedge is 45°. When the feeding hopper 52 is flipped to a horizontal state, the sliding cover 13 automatically slides downward under the action of gravity and opens (it can be opened manually in special circumstances when stuck) to facilitate feeding. When flipped to a vertical state, the sliding cover 13 will slide to cover the inclined opening to reduce the contamination of the powder by microorganisms in the air.

[0078] like Figure 1 , Figure 2 and Figure 3 As shown, preferably, two auxiliary legs 15 are symmetrically fixedly connected to one side of the mounting frame 3 , and a reinforcing plate is fixedly connected between the auxiliary legs 15 and the mounting frame 3 .

[0079] In this embodiment, auxiliary legs 15 are provided to provide auxiliary support when the mixing drum 4 is in a horizontal state, so as to reduce the pressure on the turning shaft 72 .

[0080] The present invention also provides a matrix mixing method for high-density large-scale adherent cell culture, comprising the following steps:

[0081] S1: measuring a certain amount of culture medium powder and distilled water, dividing the measured distilled water into several equal portions and adding them separately into the mixing cylinder 4;

[0082] The solution is divided into multiple independent mixing chambers. Since the solution in each mixing chamber has a smaller volume, it is easier to form turbulence during stirring, which helps to evenly disperse the powder.

[0083] S2: Measure 10% of the amount of the culture medium powder in step S1, and mix the magnetic powder and the culture medium powder according to the above ratio and add them to the mixing barrel 4 in multiple times, each time the amount of magnetic powder and the culture medium powder is equal, and each time the amount of magnetic powder and the culture medium powder is equal, and on average each portion of distilled water is added with the mixture of powder and magnetic powder 5 times;

[0084] By first adding water-insoluble magnetic powder into the powder and mixing it, and then adding it into each mixing chamber in batches, the powder is "diluted" by adding the magnetic powder, and the magnetic powder is insoluble in water, it can act as a "carrier" or "bridge" to keep a certain distance between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of clumping.

[0085] S3: applying a magnetic field to the mixing barrel 4, wherein the magnetic field is provided at a plurality of points in a ring shape around the mixing barrel 4, and the magnetic fields at adjacent points have opposite directions, and the mixing barrel 4 is controlled to flip, so that the magnetic powder flips around in the mixed liquid and reciprocates in the axial direction at the same time, until the culture medium powder is completely dissolved in the distilled water;

[0086] Magnetic fields are set on both sides of the mixing cylinder 4. After adding materials, the magnetic powder is mixed in the solution. The solution and the magnetic powder rotate around the central axis along with the rotation of the inner cylinder 42. During the process, the magnetic field composed of several groups of mixing magnets 92 makes the magnetic powder reciprocate in the axial direction of the inner cylinder 42 at the same time, so that the powder and the solution are fully mixed.

[0087] S4: Filter the mixed liquid until the magnetic powder and impurities in the mixed liquid are filtered out.

[0088] The working principle of the matrix mixing device and method for high-density large-scale adherent cell culture is described in detail below.

[0089] like Figure 1-Figure 12As shown, when the feeding trough 44 is facing the solid part of the inner wall of the outer cylinder 41 (not facing the premixing bin), the turning motor 71 is controlled to work, driving the mounting frame 3 and the mixing cylinder 4 to turn 90 degrees until the auxiliary leg 15 contacts the base 1. Correspondingly, at this time, the transmission key 76 and the side with the opening of the inner cylinder 42 are both vertically upward; then the hydraulic cylinder 63 is controlled to work, pushing the push frame 64 to move, thereby driving the cover plate 61 to open, adding a certain amount of distilled water to the inner cylinder 42, and taking a corresponding amount of powder according to the amount of distilled water added and adding it to one of the feeding hoppers 52. In a chamber, 10% of the volume of magnetic powder is measured and added to another chamber of the feeding hopper 52. After the raw materials are added, the hydraulic cylinder 63 can be controlled to retract, thereby driving the cover plate 61 to cover the inner cylinder 42. When the covering is completed, it should be ensured that: the sealing ring is squeezed so that the internal liquid cannot leak out; the sealing gasket 46 is squeezed so that the inner cylinder 42 is divided into several independent chambers by the partition plate 62, so that the amount of distilled water in each independent chamber is equal; each partition plate 62 is inserted into the insertion slot 47 on the transmission shaft 43 so that the cover plate 61 can drive the inner cylinder 42 to rotate synchronously.

[0090] After completing the above-mentioned feeding and covering actions, control the flip motor 71 to work, drive the mounting frame 3 to flip (rotate 90° in the opposite direction relative to the previous process) to a vertical state, at this time, the transmission key 76 just rotates to the inside of the key slot 77, the transmission key 76 and the key slot 77 are coaxial and fit each other, and then the delivery can begin.

[0091] During the feeding process, the electric actuator 57 is controlled to work, driving the valve core 54 to rotate. When the valve core 54 rotates until the valve cavity 55 is upward, the powder and magnetic powder in the feeding hopper 52 begin to enter the valve cavity 55 through the guide tube. The volume between the valve cavity 55 and the valve body 53 facing the magnetic powder is 10% of the volume between the valve cavity 55 and the valve body 53 facing the powder. Each time the valve core 54 rotates until the valve cavity 55 is filled, the magnetic powder taken is 10% of the powder, and the amount of powder taken (the volume between the valve cavity 55 and the valve body 53 facing the powder) is 20% of the amount of powder required in each independent chamber of the inner cylinder 42 on average, that is, five times of powder feeding is required for each chamber to complete the feeding operation.

[0092] As the valve core 54 continues to rotate, when the valve chamber 55 faces downward and the existing area is connected to the premixing chamber 58, the powder and magnetic powder will fall into the premixing chamber 58, and the mixing motor 73 is controlled to work, so that the stirring rod 59 rotates, so that the powder and the magnetic powder are simply premixed;

[0093] During the above operation, the mixing motor 73 rotates at a low speed, and the cover plate 61 and the inner cylinder 42 rotate at a low speed after passing through the reducer 74. When the feed trough 44 and the baffle 510 are rotated to coincide with each other, the baffle 510 is flipped downward to an inclined state under the action of the torsion spring. At this time, the powder and magnetic powder in the premixing chamber 58 fall into the inner cylinder 42. During the rotation of the inner cylinder 42, the baffle 510 is shaken by the shaking rod 14. Specifically, when one side of the baffle 510 contacts the shaking rod 14, the baffle 510 is flipped upward by a certain arc until Continue to rotate until the baffle 510 is separated from the shaking rod 14. At this time, the baffle 510 rotates downward again under the action of the torsion spring. There are multiple shaking rods 14, so that the baffle 510 exhibits a shaking action, thereby completely shaking the powder and magnetic powder into the inner cylinder 42, and then mixing with the distilled water; as the inner cylinder 42 continues to rotate, the part of the inner cylinder 42 without the feeding trough 44 contacts the baffle 510, and the baffle 510 is squeezed and blocked by the inner cylinder 42 at the lower end of the premixing chamber 58, so that the premixing chamber 58 can receive the powder and magnetic powder sent through the valve cavity 55 again.

[0094] After the above-mentioned addition, the magnetic powder is mixed in the solution, and rotates around the central axis of the inner cylinder 42 as the inner cylinder 42 rotates. During the process, the magnetic field formed by several groups of mixing magnets 92 makes the magnetic powder reciprocate in the axial direction of the inner cylinder 42 at the same time, so that the powder and the solution are fully mixed. In the above process, the powder is divided into multiple independent chambers for mixing, and is added in multiple times. Furthermore, each time the magnetic powder is added for "dilution", because the magnetic powder is insoluble in water, it can act as a "carrier" or "bridge" to keep a certain distance between the powder particles, thereby preventing them from agglomerating, thereby reducing the occurrence of agglomeration.

[0095] After five times of feeding (the inner cylinder 42 rotates five times), the powder and magnetic powder in the feeding hopper 52 are completely fed. At this time, the mixing motor 73 can be controlled to work at a relatively faster speed and continue mixing until the powder is completely dissolved, that is, the adherent cell culture matrix is ​​obtained. After the pH is adjusted by adding a buffer and then sterilized at high temperature and high pressure, it can be stored or used.

[0096] After the mixing is completed, the container for receiving the materials is placed under the discharge pipe 11, the electric valve 87 is opened and when the feeding trough 44 located at the bottom is connected to the discharge pipe 11, the solvent can flow out into the container through the feeding trough 44 and the discharge pipe 11. During the process, the magnetic powder and other solid impurities with larger particles are blocked on the filter screen 86 and sucked into the collecting cylinder 81 by the collecting magnet 84. The magnetic powder adsorbed on the magnet is also magnetic, so when the collecting magnet 84 adsorbs more magnetic powder, it will not affect the subsequent adsorption effect.

[0097] When the solution is discharged and the magnetic powder needs to be cleaned, the magnetic powder can be cleaned by unscrewing the collecting tube 81 and then unscrewing the end cover 83. The cleaned magnetic powder can be put into use again after being washed and dried.

[0098] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A matrix mixing device for high-density large-scale adherent cell culture, comprising a base (1), a mounting frame (3) and a mixing cylinder (4) fixedly connected to the inner side of the mounting frame (3); characterized in that: The mixing cylinder (4) comprises an outer cylinder (41) connected to the mounting frame (3) and an inner cylinder (42) rotatably connected to the outer cylinder (41); a plurality of feeding grooves (44) are provided on the side wall of the inner cylinder (42); a connecting frame (51) is fixedly connected to the side wall of the outer cylinder (41); a feeding hopper (52) is fixedly connected to the side of the connecting frame (51) away from the outer cylinder (41); a partition is provided in the middle of the feeding hopper (52); the partition divides the feeding hopper (52) into two chambers, one of which is used for feeding powder raw materials and the other is used for feeding magnetic powder; a through groove is provided on the side of the outer cylinder (41) close to the connecting frame (51); A quantitative premixing component is provided between the feeding hopper (52) and the mixing drum (4), and the quantitative premixing component is used to take a certain amount of magnetic powder and powder raw materials for premixing and then put them into the mixing drum (4); The mounting frame (3) is symmetrically and fixedly connected to two opposite sides of the mounting frame (3); a push frame (64) is fixedly connected between the output ends of the two hydraulic cylinders (63); a cover plate (61) is rotatably connected to the middle of the push frame (64); the cover plate (61) is used to cover one end of the inner cylinder (42); and a sealing ring is provided on the side of the cover plate (61) away from the push frame (64); A plurality of partition plates (62) are fixedly connected to one side of the cover plate (61) away from the push frame (64), and the partition plates (62) divide the end surface of the cover plate (61) into a plurality of sectors of equal size. A transmission shaft (43) is fixedly connected to one side of the inner wall of the inner cylinder (42), and the transmission shaft (43) is a hollow structure and has a plurality of insertion slots (47) on the side wall thereof that match the shape of the partition plates (62). A plurality of insertion rails (45) are fixedly connected to the inner side of the inner cylinder (42) at equal intervals, and the internal shape of the insertion rails (45) matches the shape of the end of the partition plates (62). A sealing gasket (46) is fixedly connected to the inner wall of the inner cylinder (42) on one side close to the transmission shaft (43), and the transmission shaft (43) and the partition plates (62) are used in coordination by plugging. A discharge pipe (11) is provided on a side of the outer cylinder (41) away from the connecting frame (51), and an electric valve (87) is installed on the discharge pipe (11); A mixing motor (73) is fixedly mounted on the top of the base (1), and the mixing motor (73) is used to drive the cover plate (61) and the inner cylinder (42) to flip.

2. The matrix mixing device for high-density large-scale adherent cell culture according to claim 1, characterized in that: The top of the base (1) is fixedly connected to a support frame (2), the inner side of the support frame (2) is rotatably connected to a flip shaft (72), the mounting frame (3) is fixedly connected to the outer side of the flip shaft (72), the top of the base (1) is fixedly installed with a flip motor (71), one end of the flip shaft (72) extends to the other side of the support frame (2) and is fixedly connected to the output end of the flip motor (71); the top of the base (1) is fixedly installed with a reducer (74), one end of the reducer (74) is connected to the output end of the mixing motor (73), the other end of the reducer (74) is fixedly connected to a main shaft (75), a key slot (77) is provided at the central position of the end of the main shaft (75), one end of the central axis of the cover plate (61) extends to the other side of the push frame (64) and is fixedly connected to a transmission key (76), the transmission key (76) and the key slot (77) have matching shapes and are used in combination.

3. The matrix mixing device for high-density large-scale adherent cell culture according to claim 2, characterized in that: The quantitative premixing component comprises a valve body (53), a premixing chamber (58) is fixedly connected to the bottom of the valve body (53), the valve body (53) is communicated with a through groove on the outer cylinder (41) through the premixing chamber (58), a stirring rod (59) is rotatably connected between the inner walls of the premixing chamber (58), a premixing motor (511) is fixedly connected to the outer wall of the premixing chamber (58), an output end of the premixing motor (511) extends into the interior of the premixing chamber (58) and is fixedly connected to one end of the stirring rod (59); two material guide pipes (56) are symmetrically arranged on the top of the valve body (53), and the top of the material guide pipe (56) is connected to the outer wall of the premixing chamber (58). The valve body (53) is connected to the feeding hopper (52); a valve core (54) is rotatably connected between the inner walls of the valve body (53); two valve chambers (55) are formed on the valve core (54); an electric actuator (57) is fixedly connected to one side of the valve body (53); an output end of the electric actuator (57) is fixedly connected to the central axis of the valve core (54); a baffle (510) is rotatably connected between the inner walls of the premixing chamber (58) via a torsion spring; a limit block (512) for limiting the position of the baffle (510) is fixedly connected to the bottom end of the inner wall of the premixing chamber (58); the baffle (510) is used in conjunction with the feeding trough (44).

4. The matrix mixing device for high-density large-scale adherent cell culture according to claim 3, characterized in that: Fixed disks (91) are provided on both sides of the mixing barrel (4), wherein one of the fixed disks (91) is fixedly connected to one side of the push frame (64), and the other fixed disk (91) is fixedly connected between the inner sides of the mounting frame (3); a plurality of mixing magnets (92) are fixedly connected between the inner walls of the fixed disks (91); the mixing magnets (92) are arranged in a ring shape, and the arcs between two adjacent mixing magnets (92) are equal; an isolation plate (93) is provided between adjacent mixing magnets (92); the mixing magnets (92) located on different fixed disks (91) correspond to each other one by one and have opposite facing magnetic poles; and the adjacent mixing magnets (92) located on the same fixed disk (91) have opposite magnetic poles on the same side.

5. The matrix mixing device for high-density large-scale adherent cell culture according to claim 4, characterized in that: The discharge pipe (11) has an S-shaped structure. A filter screen (86) is detachably connected to the inside of the discharge pipe (11). A nozzle (82) is fixedly connected to one side of the discharge pipe (11). A thread is provided on the outside of the nozzle (82) and is connected to a collecting barrel (81) via the thread. A sealing ring is provided between the collecting barrel (81) and the nozzle (82). An end cover (83) is connected to the side of the collecting barrel (81) away from the nozzle (82) via a thread. A collecting magnet (84) is fixedly connected to one side of the inner wall of the end cover (83). The collecting magnet (84) is used to absorb and collect magnetic powder in the discharge. A guide barrel (85) is fixedly connected to the side of the inner wall of the collecting barrel (81) close to the nozzle (82).

6. The matrix mixing device for high-density large-scale adherent cell culture according to claim 5, characterized in that: A plurality of material shaking rods (14) are fixedly connected to one side of the cover plate (61) close to the partition plate (62); the material shaking rods (14) cooperate with the baffle plate (510) to cause the baffle plate (510) to shake during movement.

7. The matrix mixing device for high-density large-scale adherent cell culture according to claim 6, characterized in that: A top portion of the feeding hopper (52) near one side is provided with an oblique opening, a side wall of the feeding hopper (52) near the oblique opening is symmetrically fixedly connected with slide rails (12), and a slide cover (13) is slidably connected between the two slide rails (12).

8. The matrix mixing device for high-density large-scale adherent cell culture according to claim 7, characterized in that: Two auxiliary legs (15) are symmetrically fixedly connected to one side of the mounting frame (3), and a reinforcing plate is fixedly connected between the auxiliary legs (15) and the mounting frame (3).

9. A method for mixing a matrix for high-density large-scale adherent cell culture, characterized in that: The matrix mixing device for high-density large-scale adherent cell culture according to any one of claims 1 to 8 comprises the following steps: S1: measuring a certain amount of culture medium powder and distilled water, dividing the measured distilled water into several equal portions and adding them separately into the mixing cylinder (4); S2: 5% to 10% of the amount of the culture medium powder taken in step S1 is measured as magnetic powder, and the magnetic powder and the culture medium powder are mixed according to the above ratio and added to the mixing cylinder (4) in multiple times, with the amount of magnetic powder and culture medium powder added each time being equal, and on average, the mixture of powder and magnetic powder is added at least 5 times for each portion of distilled water; S3: applying a magnetic field to the mixing barrel (4), wherein the magnetic field is provided at a plurality of points in a ring shape on the circumference of the mixing barrel (4), and the magnetic fields at adjacent points have opposite directions, and the mixing barrel (4) is controlled to flip, so that the magnetic powder flips in the circumferential direction in the mixed liquid and reciprocates in the axial direction at the same time, until the culture medium powder is completely dissolved in the distilled water; S4: Filter the mixed liquid until the magnetic powder and impurities in the mixed liquid are filtered out.

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