Preparation method of conjugated oligomer / gold nanocluster light energy conversion material

Abstract

The invention belongs to the field of optical function composite material application, and discloses a preparation method of a conjugated oligomer / gold nanocluster optical energy conversion material. The preparation method comprises the following steps: synthesizing a conjugated oligomer material of which the main chain is fluorene and thiophene and the side chain contains an active group, and compounding the conjugated oligomer material with a gold nano-cluster containing a sulfydryl ligand through covalent linkage to prepare the conjugated oligomer / gold nano-cluster compound. The preparation method provided by the invention is simple and easy to implement, two semiconductor materials are effectively compounded, the size of the nano-composite is about 2 nanometers, and the nano-composite has the capability of quickly entering photosynthetic organisms and structures. The conjugated oligomer / gold nano-cluster heterojunction is cultured with photosynthetic organisms and structures, photo-induced electrons are efficiently generated under the irradiation of sunlight and rapidly transferred into electron transport chains of the photosynthetic organisms and structures, and the conjugated oligomer / gold nano-cluster heterojunction is used for improving the solar energy conversion efficiency and increasing the biomass yield and has practical application value in the field of energy conversion.

Description

Technical Field

[0001] The invention belongs to the application field of optical functional composite materials and relates to a method for preparing a conjugated oligomer / gold nanocluster heterojunction light energy conversion material. Background Art

[0002] Solar energy is an abundant renewable energy source. Photosynthesis in nature can realize the utilization and storage of solar energy. In photosynthetic organisms, light can drive electron transfer to produce bioenergy such as bioelectricity and biofuels. However, the solar energy conversion efficiency of photosynthetic organisms is extremely low, about 3%, and photosynthesis is actually difficult to meet the existing bioenergy needs.

[0003] At present, the main technologies used to improve the solar energy conversion efficiency of photosynthetic organisms are genetic engineering and the introduction of artificial semiconductor materials. For example, overexpression of the "VPZ" structure (containing three genes related to the lutein cycle) in soybeans accelerates the switching process of the plant lutein cycle, significantly improves the photosynthesis efficiency, and increases the yield by more than 20% on average in field trials. However, due to the differences in adaptability among different species and the complex regulatory process, genetic modification technology is difficult and lacks universality. The use of artificial synthetic materials, such as organic semiconductor materials with strong light absorption properties, expands the light capture range of cyanobacteria, accelerates the electron transfer rate in light reactions, and increases the production of oxygen, NADPH and ATP in light-dependent reactions by 52.8%, 47.9% and 27.2%, respectively. Inorganic nanomaterials can also be used to simulate natural enzymes and participate in the photosynthesis process. Although artificial synthetic materials have potential in optimizing photosynthesis, they are difficult to efficiently enter the cells of photosynthetic organisms and directly enter the biosynthesis center, resulting in limited solar energy conversion efficiency.

[0004] Artificial heterojunction nanomaterials developed based on gold nanoclusters and organic semiconductor materials have structural and functional designability. Small-sized nanoclusters can quickly enter cells, thereby directly transferring exogenous photogenerated electrons to the interior of photosynthetic organisms and directly participating in photoelectric conversion. How to use this characteristic to prepare nanoheterojunction materials with high solar energy conversion efficiency and stable performance is a technology that is currently in urgent need of development, and has practical value for the application of nanocomposites in the development and utilization of biomass energy. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing a conjugated oligomer / gold nanocluster heterojunction light energy conversion material to improve the solar energy conversion efficiency of photosynthetic organisms and increase biomass yield.

[0006] The technical solution of the present invention is to synthesize a conjugated oligomer material with fluorene and thiophene as the main chain and active groups in the side chain, and to compound it with gold nanoclusters containing thiol ligands through covalent connection, so as to prepare a conjugated oligomer / gold nanocluster heterojunction, which has effective photogenerated carrier separation efficiency, and after the photogenerated electrons enter into photosynthetic organisms and structures, the photogenerated electrons can be quickly transferred to the photosynthetic electron transport chain, thereby improving the solar energy conversion efficiency and increasing the biomass yield.

[0007] The specific steps of the present invention are as follows:

[0008] (1) Synthesizing a conjugated oligomer material with fluorene and thiophene as the main chain and an active group R as the side chain, dissolving the conjugated oligomer in deionized water at a mass ratio of 1:10000-1:1000, stirring well and then dispersing by ultrasonication to obtain a conjugated oligomer solution;

[0009] (2) Mix tetrakis(hydroxymethyl)phosphonium chloride solution, chloroauric acid solution and sodium hydroxide solution (pH=8-11) in a volume ratio of 180:110:8000-180:150:8000, and then mix the above solution with thiol ligand solution in a volume ratio of 1:100-1:50, and let stand at 2-6°C for 24-48 hours to prepare a gold nanoparticle solution;

[0010] (3) mixing the gold nanoparticle solution obtained in step (2) with the sodium tetraborate solution and the mercaptoundecanoic acid ethanol solution in a volume ratio of 20:8:3 to 100:8:3, and ultrafiltration in an ultrafiltration tube to obtain a gold nanocluster solution;

[0011] (4) mixing and stirring the gold nanocluster solution obtained in step (3), the 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution and the N-hydroxysulfosuccinimide solution in a volume ratio of 100:50:50 to 100:1:1;

[0012] (5) The solution obtained in step (4) is ultrafiltered in an ultrafiltration tube, and then mixed with the oligomer solution obtained in step (1) at a volume ratio of 1:1-10:1, and then ultrafiltered in an ultrafiltration tube to obtain a conjugated oligomer / gold nanocluster light energy conversion material.

[0013] Furthermore, the main chain in step (1) is one of the main chain structures of formula ①-④, wherein the number of m is 0-10, and the number of n is 0-10.

[0014]

[0015] Furthermore, the active group R in step (1) is one or both of a carboxyl group and an amino group.

[0016] Furthermore, the thiol ligand described in step (2) is one or more of mercaptobenzoic acid, mercaptopropionic acid, mercaptohexanoic acid, disulfide ligand, mercaptoethylene glycol, thiophene derivatives, dithioethane, polythio compounds, mercaptoethanol, mercapto-p-toluenesulfonic acid, mercaptotriethylenetetramine, and glutathione.

[0017] Furthermore, the mass fraction of the tetrakis(hydroxymethyl)phosphonium chloride solution in step (2) is 0.5-5%, the concentration of chloroauric acid is 10-200 mM, and the concentration of the thiol ligand solution is 0.5-20 mM.

[0018] Furthermore, the concentration of the sodium tetraborate solution in step (3) is 1-25 mM, and the concentration of the mercaptoundecanoic acid ethanol solution is 0.01-0.2 mM.

[0019] Furthermore, the concentration of the 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution in step (4) is 0.01-0.1 mM, and the concentration of the N-hydroxysulfosuccinimide solution is 0.02-0.08 mM.

[0020] Furthermore, the ultrafiltration tube in steps (3) and (5) has a molecular weight cut-off of 300-10000, an ultrafiltration time of 0.1-0.5 hours, and an ultrafiltration centrifugal force of 2000-6000 g.

[0021] Furthermore, the mixing and stirring time of step (2) is 10-30 minutes, the mixing and stirring time of step (3) is 12-48 hours; the mixing and stirring time of step (4) is 10-30 minutes; and the mixing and stirring time of step (5) is 0.5-24 hours.

[0022] An application of a conjugated oligomer / gold nanocluster light energy conversion material. The prepared conjugated oligomer / gold nanocluster energy conversion material is co-cultured with photosynthetic organisms in a good solvent at a mass ratio of 1:400-1:10. After full action, the solar energy conversion efficiency is significantly improved and the photosynthesis of biomass is promoted.

[0023] Furthermore, the photosynthetic organism is one or more of cyanobacteria, red algae, cryptophytes, dinoflagellates, golden algae, yellow algae, diatoms, brown algae, naked algae, green algae, charophytes, photosynthetic bacteria, and higher plants.

[0024] Furthermore, the good solvent is one or more of deionized water, phosphate buffer, Tris buffer, agarose suspension, M9 buffer, LB buffer, physiological saline, BG11 medium, F / 2 medium, Walne medium, Provasoli medium, ASM-1 medium, Z8 medium, K medium, MS medium, B5 medium, White's medium, SH medium, WPM medium, NN medium, and Anderson's medium.

[0025] Furthermore, the number of days for co-cultivation in a good solvent is 0.5-7 days.

[0026] The conjugated oligomer / gold nanocluster light energy conversion material obtained by the method of the present invention has effective photogenerated carrier separation efficiency, and after entering photosynthetic organisms and structures, photogenerated electrons can be quickly transferred to the photosynthetic electron transport chain, thereby improving solar energy conversion efficiency and increasing biomass yield.

[0027] The technical features of the present invention are:

[0028] 1. By adjusting the number of thiophene and fluorene contained in the main chain of the conjugated oligomer, the type of active groups contained in the side chain of the conjugated oligomer, and the type of thiol ligands of the gold nanoclusters, the covalent connection of the conjugated oligomer and the gold nanoclusters can be successfully achieved, and the conjugated oligomer / gold nanocluster heterojunction can be endowed with photogenerated carrier separation properties.

[0029] 2. By controlling the volume ratio of the gold nanocluster solution, the 1-ethyl-(3-dimethylaminopropyl) carbodiimide solution and the N-hydroxysulfosuccinimide solution, the concentration of the 1-ethyl-(3-dimethylaminopropyl) carbodiimide solution and the N-hydroxysulfosuccinimide solution, and the volume ratio of the mixed solution to the conjugated oligomer solution, different conjugated oligomer / gold nanocluster heterojunctions can be obtained. The two semiconductor materials are effectively compounded, and the size of the nanocomposite is about 2 nanometers, and it has the ability to quickly enter photosynthetic organisms and structures, and exhibits good photogenerated electronic performance.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are: by changing the main chain structure of the conjugated oligomer, the utilization efficiency of the conjugated oligomer for solar energy is effectively increased; by covalently connecting the conjugated oligomer and the gold nanocluster to form a composite heterojunction, the photogenerated electron-hole separation efficiency is improved, and the excited state life of the gold nanocluster is effectively increased. The conjugated oligomer / gold nanocluster heterojunction is co-cultured with photosynthetic organisms, and the conjugated oligomer / gold nanocluster heterojunction can generate photogenerated electrons and transfer them to photosynthetic organisms and structures, and inject exogenous electrons to accelerate the anabolic reaction, thereby increasing the production rate and yield of biomass. The preparation method is simple and easy, and has practical application value in the field of energy conversion. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a transmission electron microscope (TEM) photograph of the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1.

[0032] Figure 2 This is a transmission electron microscope (TEM) photograph of a cyanobacteria slice after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria.

[0033] Figure 3 This is the instantaneous photocurrent density curve of cyanobacteria after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria.

[0034] Figure 4 This is a bar graph of lipid content of cyanobacteria before and after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria. DETAILED DESCRIPTION

[0035] Example 1

[0036] (1) Synthesizing a conjugated oligomer material whose main chain is fluorene-thiophene-fluorene, whose thiophene side chain contains amino group and whose fluorene side chain contains carboxyl group, dissolving the conjugated oligomer in deionized water at a mass ratio of 1:5000, stirring well and then dispersing by ultrasonication to obtain a conjugated oligomer solution;

[0037]

[0038] (2) 1% tetrakis(hydroxymethyl)phosphonium chloride solution, 50 mM chloroauric acid solution and sodium hydroxide solution (pH=9) were mixed and stirred at a volume ratio of 180:137:8000 for 15 minutes, and then the above solution was mixed and stirred with 10 mM mercaptobenzoic acid solution at a volume ratio of 1:80 for 15 minutes, and allowed to stand at 4°C for 24 hours to prepare a gold nanoparticle solution;

[0039] (3) mixing the gold nanoparticle solution obtained in step (2) with a 5 mM sodium tetraborate solution and a 0.1 mM mercaptoundecanoic acid ethanol solution in a volume ratio of 40:8:3 and stirring for 24 hours, and ultrafiltration at a centrifugal force of 4000 g for 20 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a gold nanocluster solution;

[0040] (4) mixing the gold nanocluster solution obtained in step (3), 0.04 mM 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution and 0.02 mM N-hydroxysulfosuccinimide solution in a volume ratio of 100:10:10 and stirring for 15 minutes;

[0041] (5) ultrafiltration of the solution obtained in step (4) at a centrifugal force of 4000 g for 10 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000, and then mixing with the oligomer solution obtained in step (1) at a volume ratio of 4:1 and stirring for 4 hours, and ultrafiltration of the solution in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000 g for 10 minutes to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e., a conjugated oligomer / gold nanocluster light energy conversion material;

[0042] (6) The conjugated oligomer / gold nanocluster heterojunction was co-cultured with cyanobacteria in deionized water at a mass ratio of 1:25 for 7 days.

[0043] Performance Testing:

[0044] Figure 1 is a transmission electron microscope (TEM) photograph of the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1. Figure 1 It can be seen that the particle size of the heterojunction is uniform, about 2 nm, and is well dispersed.

[0045] Figure 2 1 is a transmission electron microscope (TEM) photograph of a cyanobacteria slice after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria. Figure 2 It can be seen that the conjugated oligomer / gold nanocluster heterojunction can enter the interior of cyanobacteria and has good dispersion in the cells.

[0046] Figure 3 is the instantaneous photocurrent density curve of cyanobacteria after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria. Figure 3 It can be seen that after co-cultivation with the conjugated oligomer / gold nanocluster heterojunction, the photocurrent of cyanobacteria reached 50 nA / cm 2 , confirming that the conjugated oligomer / gold nanocluster heterojunction can improve the light energy conversion efficiency of cyanobacteria.

[0047] Figure 4 : is a bar graph of lipid content of cyanobacteria before and after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with cyanobacteria. Figure 4 It can be seen that after co-cultivation with conjugated oligomer / gold nanocluster heterojunction, the lipid content of cyanobacteria increased by 19.26%, promoting the increase of cyanobacterial biomass yield.

[0048] Example 2

[0049] Steps (1) to (5) are the same as in Example 1;

[0050] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction with cyanobacteria in deionized water at a mass ratio of 1:25 for 7 days. The lipid content of the green algae increased by 16.74%, and the protein content increased by 27.25%, which promoted the increase of the green algae biomass yield.

[0051] Example 3

[0052] (1) Synthesizing a conjugated oligomer material whose main chain is fluorene-thiophene-thiophene-fluorene, whose thiophene side chain contains amino group and whose fluorene side chain contains carboxyl group, dissolving the conjugated oligomer in deionized water at a mass ratio of 1:4000, stirring well and then dispersing by ultrasonication to obtain a conjugated oligomer solution;

[0053]

[0054] (2) 1% tetrakis(hydroxymethyl)phosphonium chloride solution, 50 mM chloroauric acid solution and sodium hydroxide solution (pH=9) were mixed and stirred at a volume ratio of 180:137:8000 for 15 minutes, and then the above solution was mixed and stirred with 10 mM glutathione solution at a volume ratio of 1:80 for 15 minutes, and allowed to stand at 4°C for 24 hours to prepare a gold nanoparticle solution;

[0055] (3) mixing the gold nanoparticle solution obtained in step (2) with a 5 mM sodium tetraborate solution and a 0.1 mM mercaptoundecanoic acid ethanol solution in a volume ratio of 50:8:3 and stirring for 48 hours, and ultrafiltration at a centrifugal force of 4000 g for 20 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a gold nanocluster solution;

[0056] (4) mixing the gold nanocluster solution obtained in step (3), 0.04 mM 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution and 0.02 mM N-hydroxysulfosuccinimide solution in a volume ratio of 100:20:20 and stirring for 15 minutes;

[0057] (5) The solution obtained in step (4) was ultrafiltered in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000 g for 10 minutes, and then mixed with the oligomer solution obtained in step (1) at a volume ratio of 2:1 and stirred for 3 hours, and ultrafiltered in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000 g for 10 minutes to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e., a conjugated oligomer / gold nanocluster light energy conversion material;

[0058] (6) The conjugated oligomer / gold nanocluster heterojunction was co-cultured with cyanobacteria in deionized water at a mass ratio of 1:10 for 7 days. The photocurrent of the cyanobacteria reached 52 nA / cm 2, improving the light energy conversion efficiency of cyanobacteria. The lipid content of cyanobacteria increased by 19.98%, promoting the increase of cyanobacteria biomass production.

[0059] Example 4

[0060] Steps (1) to (5) are the same as those in Example 3;

[0061] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction with cyanobacteria in deionized water at a mass ratio of 1:50 for 7 days. The photocurrent of cyanobacteria reached 43 nA / cm 2 , improving the light energy conversion efficiency of cyanobacteria. The lipid content of cyanobacteria increased by 16.12%, promoting the increase of cyanobacteria biomass production.

[0062] Example 5

[0063] (1) Synthesizing a conjugated oligomer material having a main chain of thiophene-fluorene-thiophene, an amino group on the thiophene side chain, and a carboxyl group on the fluorene side chain, dissolving the conjugated oligomer in deionized water at a mass ratio of 1:6000, stirring well and then dispersing by ultrasonication to obtain a conjugated oligomer solution;

[0064]

[0065] (2) 1% tetrakis(hydroxymethyl)phosphonium chloride solution, 50 mM chloroauric acid solution and sodium hydroxide solution (pH=9) were mixed and stirred at a volume ratio of 180:137:8000 for 20 minutes, and then the above solution was mixed and stirred with 10 mM mercaptobenzoic acid solution at a volume ratio of 1:80 for 20 minutes, and allowed to stand at 4°C for 30 hours to prepare a gold nanoparticle solution;

[0066] (3) mixing the gold nanoparticle solution obtained in step (2) with a 5 mM sodium tetraborate solution and a 0.1 mM mercaptoundecanoic acid ethanol solution in a volume ratio of 40:8:3 and stirring for 48 hours, and ultrafiltration at a centrifugal force of 4000 g for 20 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a gold nanocluster solution;

[0067] (4) mixing the gold nanocluster solution obtained in step (3), 0.04 mM 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution and 0.02 mM N-hydroxysulfosuccinimide solution in a volume ratio of 100:5:5 and stirring for 15 minutes;

[0068] (5) The solution obtained in step (4) was ultrafiltered in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000 g for 10 minutes, and then mixed with the oligomer solution obtained in step (1) at a volume ratio of 4:1 and stirred for 5 hours, and ultrafiltered in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000 g for 10 minutes to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e., a conjugated oligomer / gold nanocluster light energy conversion material;

[0069] (6) The conjugated oligomer / gold nanocluster heterojunction was co-cultured with cyanobacteria in deionized water at a mass ratio of 1:25 for 7 days. The photocurrent of the cyanobacteria reached 37 nA / cm 2 , improving the light energy conversion efficiency of cyanobacteria. The lipid content of cyanobacteria increased by 14.06%, promoting the increase of cyanobacteria biomass production.

[0070] Example 6

[0071] Steps (1) to (5) are the same as in Example 5;

[0072] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction with cyanobacteria in deionized water at a mass ratio of 1:50 for 7 days. The photocurrent of cyanobacteria reached 20 nA / cm 2 , improving the light energy conversion efficiency of cyanobacteria. The lipid content of cyanobacteria increased by 11.81%, promoting the increase of cyanobacteria biomass production.

[0073] The above are only embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included within the scope of the claims of the present application.

Claims

1. A method for preparing a conjugated oligomer / gold nanocluster light energy conversion material, characterized in that: The specific steps are as follows: (1) Synthesizing a conjugated oligomer material with fluorene and thiophene as the main chain and an active group R as the side chain, dissolving the conjugated oligomer in deionized water at a mass ratio of 1:10000-1:1000, stirring well and then dispersing by ultrasonication to obtain a conjugated oligomer solution; (2) Mixing tetrakis(hydroxymethyl)phosphonium chloride solution, chloroauric acid solution and sodium hydroxide solution at a volume ratio of 180:110:8000-180:150:8000, wherein the pH value of the sodium hydroxide solution is 8-11, and then mixing the above solution with the thiol ligand solution at a volume ratio of 1:100-1:50, and standing at 2-6°C for 24-48 hours to prepare a gold nanoparticle solution; (3) mixing the gold nanoparticle solution obtained in step (2) with the sodium tetraborate solution and the mercaptoundecanoic acid ethanol solution in a volume ratio of 20:8:3 to 100:8:3, and ultrafiltration in an ultrafiltration tube to obtain a gold nanocluster solution; (4) mixing and stirring the gold nanocluster solution obtained in step (3), the 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution and the N-hydroxysulfosuccinimide solution in a volume ratio of 100:50:50 to 100:1:1; (5) The solution obtained in step (4) is ultrafiltered in an ultrafiltration tube, and then mixed with the oligomer solution obtained in step (1) at a volume ratio of 1:1-10:1, and then ultrafiltered in an ultrafiltration tube to obtain a conjugated oligomer / gold nanocluster light energy conversion material.

2. The preparation method according to claim 1, characterized in that: The main chain in step (1) is one of the main chain structures of formula ① to ④, wherein the number of m is 0-10, the number of n is 0-10, 。 3. The preparation method according to claim 1, characterized in that: The active group R in step (1) is one or both of a carboxyl group and an amino group.

4. The preparation method according to claim 1, characterized in that: The thiol ligand described in step (2) is one or more of mercaptobenzoic acid, mercaptopropionic acid, mercaptohexanoic acid, disulfide ligand, mercaptoethylene glycol, thiophene derivatives, dithioethane, polythio compounds, mercaptoethanol, mercapto-p-toluenesulfonic acid, mercaptotriethylenetetramine, and glutathione.

5. The preparation method according to claim 1, characterized in that: The mass fraction of the tetrakis(hydroxymethyl)phosphonium chloride solution in step (2) is 0.5-5%, the concentration of chloroauric acid is 10-200 mM, and the concentration of the thiol ligand solution is 0.5-20 mM.

6. The preparation method according to claim 1, characterized in that: The concentration of the sodium tetraborate solution in step (3) is 1-25 mM, and the concentration of the mercaptoundecanoic acid ethanol solution is 0.01-0.2 mM.

7. The preparation method according to claim 1, characterized in that: The concentration of the 1-ethyl-(3-dimethylaminopropyl)carbodiimide solution in step (4) is 0.01-0.1 mM, and the concentration of the N-hydroxysulfosuccinimide solution is 0.02-0.08 mM.

8. The preparation method according to claim 1, characterized in that: The ultrafiltration tube described in steps (3) and (5) has a molecular weight cutoff of 300-10000, an ultrafiltration time of 0.1-0.5 hours, and an ultrafiltration centrifugal force of 2000-6000 g.

9. The preparation method according to claim 1, characterized in that: The mixing and stirring time of step (2) is 10-30 minutes, the mixing and stirring time of step (3) is 12-48 hours; the mixing and stirring time of step (4) is 10-30 minutes; and the mixing and stirring time of step (5) is 0.5-24 hours.

10. Use of a conjugated oligomer / gold nanocluster light energy conversion material obtained by the preparation method according to any one of claims 1 to 8, characterized in that: The conjugated oligomer / gold nanocluster energy conversion material is co-cultured with photosynthetic organisms in a good solvent at a mass ratio of 1:400-1:

10.

11. The use of the conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that: The photosynthetic organisms are one or more of cyanobacteria, red algae, cryptophytes, dinoflagellates, golden algae, yellow algae, diatoms, brown algae, naked algae, green algae, charophytes, photosynthetic bacteria, and higher plants.

12. The use of the conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that: The good solvent is one or more of deionized water, phosphate buffer, Tris buffer, agarose suspension, M9 buffer, LB buffer, physiological saline, BG11 medium, F / 2 medium, Walne medium, Provasoli medium, ASM-1 medium, Z8 medium, K medium, MS medium, B5 medium, White's medium, SH medium, WPM medium, NN medium, and Anderson's medium.

13. The use of the conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that: The number of days for co-cultivation in a good solvent is 0.5-7 days.

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