Avermectin streptomyces-purpureocillium lilacinum complex microbial inoculant as well as preparation device and method thereof

Abstract

The invention discloses an abamectin streptomyces-purpureocillium lilacinum composite microbial agent and a preparation device and method. The abamectin streptomyces-purpureocillium lilacinum composite microbial agent comprises a strain preparation module, a culture medium configuration module, a composite fermentation module and a centrifugal purification module, the compound fermentation module comprises a fermentation tank, a detection mechanism for detecting a compound microbial agent, a control mechanism for controlling a measurement time interval and a cleaning mechanism; the detection mechanism comprises a detection frame arranged in the fermentation tank in a lifting manner, a plurality of detection cylinders fixed on the detection frame, a protection cylinder rotationally arranged outside the detection cylinders, a pH sensor arranged in the detection cylinders and a driving assembly used for controlling the protection cylinder to rotate, the detection cylinders are provided with detection holes, and the protection cylinder is provided with protection holes; the inner wall of the protection cylinder blocks the detection hole when the protection cylinder is at the initial position; the cleaning mechanism is used for cleaning the pH sensor. The compound bacteria disclosed by the invention combine respective advantages of three microorganisms, and can be used for more widely preventing and treating various pests, nematodes and pathogenic bacteria.

Description

Technical Field

[0001] The present application relates to the technical field of composite microbial agents, and in particular to a composite microbial agent of Streptomyces avermectinus and Pseudomonas lilacinus and a preparation device and method thereof. Background Art

[0002] At present, root-knot nematodes directly harm the roots of vegetables, causing tumor-like protrusions on the roots of affected crops, poor growth, and root rot. Because the root system is affected, most affected plants initially have slow growth of the aboveground parts, smaller and yellower leaves, spots of lack of fertilizer, no or poor fruiting, and in severe cases, growth stagnation, shortened internodes, dwarfed plants, or even wilting.

[0003] There are many existing single microorganisms, but none of them can achieve the purpose of insect control and growth promotion. In the process of insect control and growth promotion, composite microorganisms that can achieve the purpose of insect control and growth promotion are needed. Therefore, people have studied the composite bacterial agent of Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus. Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus are three different microorganisms, which have their own unique functions in agriculture, biological control and other fields. When these three microorganisms are combined in the form of composite bacteria, they may produce a wider range of biological control effects and more significant agricultural application advantages.

[0004] The preparation method of the composite microbial agent includes the preparation of strains, the configuration of culture medium, composite fermentation and subsequent treatment. The following is a general preparation method: 1. Preparation of strains: Streptomyces avermectin: obtain a high-yield avermectin Streptomyces strain from a preservation institution or laboratory. Ensure the purity and activity of the strain, and perform rejuvenation and screening when necessary. Psoralea lilacinus: also obtain a pure strain of Psoralea lilacinus from a preservation institution or laboratory. Check the biological characteristics and functions of the strain to ensure that it has the expected prevention and control effect. Brevibacillus laterosporus: obtain a strain of Brevibacillus laterosporus to ensure that it has good growth and reproduction ability. 2. Culture medium configuration: Basic culture medium: configure a basic culture medium suitable for the co-growth of three microorganisms. The culture medium should contain an appropriate amount of carbon source, nitrogen source, inorganic salts and necessary trace elements. It can be appropriately adjusted according to the nutritional requirements of each strain to achieve the best composite fermentation effect. Optimize the culture medium: optimize the formula and conditions of the culture medium through experiments to improve the yield and activity of the composite bacteria. Considering the demand of Streptomyces avermectinosum for the synthesis of avermectin, specific precursors or inducers can be added to the culture medium. 3. Composite fermentation: Inoculation: The prepared Streptomyces avermectinosum, Pseudomonas lilacinus and Bacillus laterosporus strains are inoculated into the basal culture medium in a certain proportion. The inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure that they can grow in a coordinated manner during the composite fermentation process. Fermentation condition control: Control the temperature, pH value, dissolved oxygen content and other parameters during the fermentation process to create an environment suitable for the co-growth of the three microorganisms. Sampling and testing are carried out regularly to observe the growth of microorganisms and the accumulation of metabolites. Fermentation cycle: Determine the optimal fermentation cycle based on the experimental results. Generally speaking, the cycle of composite fermentation will be longer than that of a single strain, because the interaction between different strains and the accumulation of metabolites need to be considered. 4. Subsequent treatment: Separation and purification: After the fermentation is completed, the bacteria and the fermentation liquid are separated by centrifugation, filtration and other methods. Quality inspection: The extracted and purified products are quality inspected to ensure that they meet the relevant standards and requirements.

[0005] Regarding the above-mentioned related technologies, the inventors believe that the following defects exist: the pH value of the bacterial agent needs to be acidic during the fermentation process, but in the process of preparing the bacterial agent, the pH value is not monitored, so the pH value cannot be adjusted in time, affecting the quality of the finished product. Summary of the invention

[0006] In order to improve the problem that the pH value cannot be monitored during the fermentation process, the present application provides a Streptomyces avermectin-Purplesporium lilacinum composite bacterial agent and a preparation device and method.

[0007] The present application provides a preparation device for a composite bacterial agent of Streptomyces avermectinosum and Pseudomonas lilacinus, which adopts the following technical scheme: A preparation device for a composite bacterial agent of Streptomyces avermectinosum and Pseudomonas lilacinus, comprising a strain preparation module, a culture medium configuration module, a composite fermentation module and a centrifugal purification module, wherein the composite fermentation module comprises a fermentation tank, a detection mechanism for detecting the composite bacterial agent, a control mechanism for controlling a measurement time interval and a cleaning mechanism; The detection mechanism includes a detection frame that is lifted and lowered in the fermentation tank, a plurality of detection cylinders fixed on the detection frame, a protective cylinder that is rotatably arranged outside the detection cylinder, a pH sensor arranged in the detection cylinder, and a driving component for controlling the rotation of the protective cylinder. The detection cylinder is provided with a detection hole, and the protective cylinder is provided with a protective hole. When the protective cylinder is in an initial position, the inner wall of the protective cylinder blocks the detection hole; the cleaning mechanism is used to clean the pH sensor.

[0008] Optionally, the cleaning mechanism includes a cleaning rack that is lifted and lowered in the protective tube, a cleaning component for cleaning the pH sensor, an accelerating component for accelerating the cleaning of the pH sensor, and a circulation component for realizing water circulation in the detection tube.

[0009] Optionally, the cleaning assembly includes two wiping members elastically arranged on the cleaning frame and a driving unit for realizing elastic movement of the two wiping members, and the two wiping members are respectively located on both sides of the pH sensor.

[0010] Optionally, the driving part includes two slides, two first springs, two first electromagnets and two driving members, the wiping member is fixed on the slide, the two first springs are used to realize the elastic setting of the two slides on the cleaning rack, the driving member is used to control the reciprocating motion of the first electromagnet, and the first electromagnet is used to adsorb the slide.

[0011] Optionally, the acceleration assembly includes a storage tank, an acceleration tube, a solenoid valve, a phase change ball and a power unit for driving the phase change ball to move. The storage tank is installed on the inner wall of the fermentation tank, one end of the acceleration tube is connected to the storage tank, and the other end is connected to the detection cylinder. The storage tank is filled with a cleaning agent for cleaning the pH sensor. The storage tank is provided with a phase change hole, which is used to place the phase change ball. The phase change ball is filled with phase change material.

[0012] Optionally, the power unit includes a first pipe, a second pipe, a third pipe, an elastic rod and a push rod. The bottom end of the first pipe is closed, the elastic rod is elastically arranged at the bottom end of the first pipe, the second pipe is arranged in an arc shape and one end is connected to one end of the first pipe, and the other end is opposite to the opening of the phase change hole, one end of the third pipe is opposite to the phase change hole, and the other end is connected to the bottom end of the first pipe, and the push rod is slidably arranged on the storage tank.

[0013] Optionally, the control mechanism includes a control frame rotatably arranged outside the fermentation tube, a top box, a bottom box, a liquid level sensor, a regulating tube and a regulating component for controlling the flow in the regulating tube, the regulating tube is arranged as a hose, the regulating tube is in a stretched state, the liquid level sensor is installed in the bottom box, the top box and the bottom box are fixedly connected to the control frame, and the regulating tube is used to connect the top box and the bottom box.

[0014] Optionally, the adjustment assembly includes an adjustment block, an adjustment rod, an adjustment wheel and an adjustment part fixed on the control frame, the adjustment block is provided with a through hole for the adjustment tube to pass through, the adjustment block is also provided with an adjustment groove, the adjustment groove is inclined, and both side walls of the adjustment groove are provided with inclined grooves, the two ends of the adjustment rod slide in the two inclined grooves respectively, the adjustment wheel is rotatably provided outside the adjustment rod and is located in the adjustment groove, and the flow rate of the adjustment tube is maximum or minimum when the adjustment rod is located at the two ends of the inclined groove.

[0015] The preparation method of a composite bacterial agent of Streptomyces avermectinosum and Pseudomonas lilacinus provided in the present application adopts the following technical scheme: A method for preparing a composite bacterial agent of Streptomyces avermectinosum and Pseudomonas lilacinus comprises the following steps: S1. Preparation of bacterial strains: Take out Streptomyces avermectin, Pseudomonas lilacinus, and Brevibacillus laterosporus to ensure that the bacterial strains have good activity; S2. Medium configuration: Optimize the formula and conditions of the medium through experiments to increase the yield and activity of the composite bacteria; S3: composite fermentation: inoculation: inoculating the prepared Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus strains into the basal medium in a certain proportion; S4: Fermentation condition control: Control the temperature, pH value, dissolved oxygen content and other parameters during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; the detection mechanism is used to monitor and control the pH value in real time; S5: Separation and purification: After the fermentation is completed, the bacteria and the fermentation liquid are separated by centrifugation, filtration and other methods to obtain the composite bacterial agent.

[0016] The present application provides a composite bacterial agent of Streptomyces avermectinus and Pseudomonas lilacinus, which adopts the following technical scheme: A composite bacterial agent of Streptomyces avermectins and Pseudomonas lilacinus comprises Streptomyces avermectins, Pseudomonas lilacinus and Brevibacillus laterosporus.

[0017] In summary, the present application includes at least one of the following beneficial technical effects: 1. Composite bacteria combine the advantages of three microorganisms and can more widely control a variety of pests, nematodes and pathogens. There may be synergistic effects between different microorganisms to jointly improve the control effect. For example, Pseudomonas lilacinus and Brevibacillus laterosporus may jointly inhibit the growth of nematodes and pathogens through different mechanisms. The use of composite bacteria can reduce the drug resistance problem caused by the continuous use of single microbial preparations. Finally, Pseudomonas lilacinus and Brevibacillus laterosporus can produce physiologically active substances that promote plant growth. The use of composite bacteria may more significantly promote plant growth and development. The composite bacteria of Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus has broad application prospects and significant advantages in agriculture; 2. After the driving motor controls the driving gear to rotate, the driving gear controls the ring gear to rotate, and the ring gear drives the protective tube to rotate, thereby realizing the rotation effect of the protective tube, and starting to use the composite bacterial agent, and detecting the composite bacterial agent through the pH sensor until the pH value meets the requirements; after the detection is completed, the pH sensor needs to be cleaned, and water is injected into the detection tube, and the liquid surface of the water does not need to completely submerge the pH sensor. The wiping piece can absorb water, and the wiping piece impacts the pH sensor through the reciprocating motion of the wiping piece. In this embodiment, the distance between the wiping piece and the pH sensor is short and due to the water resistance, the impact force of the wiping piece on the pH sensor is small, and the pH sensor will not be damaged. The wiping piece drives the water to fluctuate, and as the wiping piece and the pH sensor are impacted and wiped and the water fluctuates and impacts, the solution adhering to the pH sensor is greatly accelerated to fall off; 3. In the early stage of pH adjustment in the fermentation tank, it is necessary to shorten the measurement time interval, so it is necessary to accelerate the cleaning effect of the pH sensor. At this time, the adjustment rod needs to be pulled to the top of the adjustment tank. At this time, the adjustment tube is in a fully open state. In this embodiment, this time can be set as the time for cleaning the pH sensor with clean water. When the liquid level sensor detects that the water level in the bottom box rises to the top, the phase change ball is immediately controlled to move into the phase change hole. The detergent in the storage tank is heated, and the repeated stirring of the two wipers greatly accelerates the mixing effect of the detergent, and greatly accelerates the efficiency of cleaning the pH sensor. Finally, it is also necessary to swap the position of the top box and the bottom box, and adjust the tube to fully open the flow rate to quickly flow the water in the bottom box into the top box to time the next cleaning work. In the middle and late stages of pH adjustment in the fermentation tank, it is only necessary to move the adjustment rod and the adjustment wheel to the middle position of the adjustment tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a process flow chart of Example 2 of the present application; Figure 2 It is a schematic diagram of the overall structure of the first embodiment of the present application; Figure 3 It is a schematic diagram of the detection mechanism, the control mechanism and the cleaning mechanism in the first embodiment of the present application; Figure 4 is a schematic diagram of a cleaning component in Example 1 of the present application; Figure 5 It is a schematic diagram of the push rod and the storage tank in the first embodiment of the present application; Figure 6 yes Figure 2 The enlarged view of point A in the middle; Figure 7 It is a schematic diagram of the adjustment component in Example 1 of the present application.

[0019] Figure numerals: 1, fermentation tank; 2, detection rack; 3, detection cylinder; 4, protection cylinder; 5, pH sensor; 6, detection hole; 7, protection hole; 8, cleaning rack; 9, wiper; 10, slide plate; 11, first spring; 12, first electromagnet; 13, circulation box; 14, circulation pump; 15, water pipe; 16, drive motor; 17, drive gear; 18, ring gear; 19, slide rod; 20, storage tank; 21, acceleration tube; 22, solenoid valve; 23, phase change ball; 24, Phase change hole; 25, first pipeline; 26, second pipeline; 27, third pipeline; 28, elastic rod; 29, push rod; 30, power electric push rod; 31, handle; 32, power electromagnet; 33, power spring; 34, driving member; 35, control frame; 36, top box; 37, bottom box; 38, liquid level sensor; 39, adjusting tube; 40, adjusting block; 41, adjusting rod; 42, adjusting wheel; 43, adjusting member; 44, perforation; 45, adjusting slot; 46, inclined slot. DETAILED DESCRIPTION

[0020] The following is combined with Figure 1-7 This application is described in further detail.

[0021] Embodiment 1

[0022] The present application embodiment discloses a preparation device for a composite bacterial agent of Streptomyces avermectinus and Pseudomonas lilacinus. Figure 2-Figure 5The preparation device of the composite bacterial agent of Streptomyces avermectinosum and Bacillus lilacinus includes a strain preparation module, a culture medium configuration module, a composite fermentation module and a centrifugal purification module. In the strain preparation, Streptomyces avermectinosum, Bacillus lilacinus and Brevibacillus laterosporus need to be selected, and it is necessary to ensure that each strain is in good biological performance and that each strain can grow and reproduce. The culture medium is used to cultivate the composite bacterial agent, and it is necessary to ensure an appropriate amount of carbon source, nitrogen source, inorganic salt and necessary trace elements, which can be appropriately adjusted according to the nutritional requirements of each strain to achieve the best composite fermentation. Effect: During the composite fermentation process, the prepared strains of Streptomyces avermectin, Pseudomonas lilacinus and Bacillus laterosporus need to be inoculated into the basic culture medium in a certain proportion. The inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure that they can grow in a coordinated manner during the composite fermentation process. The temperature, pH value, dissolved oxygen content and other parameters during the fermentation process should be controlled to create an environment suitable for the co-growth of the three microorganisms. Sampling and testing should be carried out regularly to observe the growth of microorganisms and the accumulation of metabolites. After the fermentation is completed, the bacteria and the fermentation liquid are separated by centrifugation, filtration and other methods. The extracted and purified products are quality tested to ensure that they meet the relevant standards and requirements, and the composite bacterial preparation is promoted and applied in agriculture, horticulture and other fields to exert its broad-spectrum, efficient, safe and long-term biological control effect.

[0023] Reference Figure 2-Figure 5 The composite fermentation module includes a fermentation tank 1, a detection mechanism for detecting the composite bacterial agent, a control mechanism for controlling the measurement time interval, and a cleaning mechanism; especially in the early fermentation process, the pH value changes greatly at this time, so the measurement time interval is shortened, and after the fermentation reaches the middle and late stages, the measurement time interval can be appropriately lengthened, so it is necessary to control the measurement interval time through the control mechanism, and the detection structure detects the pH value of the composite bacterial agent. After each detection is completed, the cleaning mechanism is required to clean the detection mechanism to avoid incorrect detection results due to incomplete cleaning.

[0024] The detection mechanism includes a detection frame 2 that is lifted and arranged in the fermentation tank 1, a plurality of detection cylinders 3 fixed on the detection frame 2, a protective cylinder 4 that is rotatably arranged outside the detection cylinder 3, a pH sensor 5 arranged in the detection cylinder 3, and a driving component for controlling the rotation of the protective cylinder 4. The detection cylinder 3 is provided with a detection hole 6, and the protective cylinder 4 is provided with a protection hole 7. The lifting and lowering of the detection frame 2 is achieved by an electric push rod. In this embodiment, it is necessary to select multiple detection cylinders 3 to detect different depths of the composite bacterial agent. However, since the structure of each detection cylinder 3 only differs in position, this embodiment and related drawings are schematically and explained with one detection cylinder 3. A rotating ring is fixedly connected to the inner wall of the protective cylinder 4, and a rotating groove is provided on the outer wall of the detection cylinder 3. The rotating ring rotates in the rotating groove, thereby realizing the rotation effect of the protective cylinder 4 and the detection cylinder 3. The protective cylinder 4 is in the initial position The inner wall of the protective tube 4 blocks the detection hole 6. Only when the protective hole 7 rotates to the position of the detection hole 6, the solution enters the detection hole 6 from the protective hole 7. The driving assembly includes a driving motor 16, a driving gear 17 and a ring gear 18. The driving motor 16 is fixed on the detection frame 2. The detection frame 2 is also provided with a protective box for protecting the driving motor 16. The driving gear 17 is fixedly connected to the output end of the driving motor 16, and the ring gear 18 is fixedly connected to the outer peripheral wall of the protective tube 4. The driving gear 17 is meshed with the ring gear 18. After the driving motor 16 controls the driving gear 17 to rotate, the driving gear 17 controls the ring gear 18 to rotate, and the ring gear 18 drives the protective tube 4 to rotate, thereby realizing the rotation effect of the protective tube 4, and starting to use the composite bacterial agent, and detecting the composite bacterial agent through the pH sensor 5 until the pH value meets the requirements.

[0025] Reference Figure 2-Figure 5 The cleaning mechanism is used to clean the pH sensor 5. The cleaning mechanism includes a cleaning rack 8 that is lifted and arranged in the protective tube 4, a cleaning component for cleaning the pH sensor 5, an accelerating component for accelerating the cleaning of the pH sensor 5, and a circulating component for realizing water circulation in the detection tube 3. The cleaning rack 8 is arranged in a concave shape, and a groove for the pH sensor 5 to pass through is arranged at the horizontal part of the cleaning rack 8. The lifting and lowering of the cleaning rack 8 is realized by an electric push rod or a cylinder. The cleaning component cleans the pH sensor 5. The accelerating component is used to accelerate the cleaning speed of the pH sensor 5. The circulating component is used to circulate the cleaning water in the protective tube 4. The circulating component includes a circulating water tank, a circulating pump 14 and two water pipes 15. One end of one water pipe 15 is connected to the circulating pump 14, and the other end thereof is connected to the top of the detection tube 3. One end of the other water pipe 15 is connected to the bottom of the detection tube 3, and the other end thereof is connected to the circulating water tank. The circulating pump 14 is installed in the circulating water tank. The circulating water tank is placed in the external environment and its position is relative to the position of the detection tube 3, so as to realize the effect of water circulation.

[0026] Reference Figure 2-Figure 5The cleaning assembly includes two wiping members 9 elastically arranged on the cleaning rack 8 and a driving part for realizing the elastic movement of the two wiping members 9. The two wiping members 9 are respectively located on both sides of the pH sensor 5. The wiping members 9 in this embodiment are sponge blocks, which are used to clean the pH sensor 5. The driving part includes two first slides 10, two first springs 11, two first electromagnets 12 and two driving members 34. The slide 10 is elastically arranged on the cleaning rack 8, and the two slides 10 are respectively located on both sides of the pH sensor 5. The wiping members 9 are fixed on the side of the slide 10 facing the pH sensor 5. The two first springs 11 are used to realize the elastic arrangement of the two slides 10 on the cleaning rack 8. One end of the first spring 11 is fixedly connected to the back of the slide 10, and the other end is fixedly connected to the cleaning rack 8. Of course, the slide 10 can also be fixedly connected with a slide rod 19. A sliding hole is provided for the sliding rod 19 to pass through, and the cooperation between the sliding rod 19 and the sliding hole is used to limit the movement direction of the slide plate 10. The driving member 34 is used to control the reciprocating motion of the first electromagnet 12, and the first electromagnet 12 is used to adsorb the slide plate 10. The driving member 34 is selected from an electric push rod or a cylinder; when water is injected into the detection cylinder 3, and the liquid level of the water does not need to completely submerge the pH sensor 5, the wiping member 9 can adsorb water, and through the reciprocating motion of the wiping member 9, the wiping member 9 impacts the pH sensor 5. In this embodiment, the collision distance between the wiping member 9 and the pH sensor 5 is short and due to the water resistance, the impact force of the wiping member 9 on the pH sensor 5 is small, and the pH sensor 5 will not be damaged. The wiping member 9 drives the water to fluctuate. As the wiping member 9 and the pH sensor 5 are impacted and wiped and the water fluctuates and impacts, the solution adhering to the pH sensor 5 is greatly accelerated to fall off.

[0027] The acceleration assembly includes a storage tank 20, an acceleration tube 21, a solenoid valve 22, a phase change ball 23 and a power unit for driving the phase change ball 23 to move. The phase change ball 23 is filled with a phase change material that is reversible in absorbing and releasing heat. The storage tank 20 is installed on the inner wall of the fermentation tank 1. One end of the acceleration tube 21 is connected to the storage tank 20, and the other end is connected to the detection cylinder 3. The length of the acceleration tube 21 is sufficient to detect the movement of the detection cylinder 3 and the acceleration tube 21 is made of soft material. The storage tank 20 is filled with a detergent for cleaning the pH sensor 5. The storage tank 20 can also be provided with an acceleration pump connected to the acceleration tube 21 to accelerate the detergent to enter the storage tank 20; the storage tank 20 is provided with a phase change hole 24, the storage tank 20 is placed vertically, and the phase change hole 24 is horizontally oriented. During the pH adjustment process of the fermentation tank 1, an acid-base neutralization reaction occurs, and heat is generated during the acid-base neutralization process. At this time, the phase change ball 23 begins to absorb heat. When the phase change ball 23 moves into the phase change hole 24, in order to accelerate the heat release rate of the phase change ball 23, the storage tank 20 is made of a material with a larger conductor coefficient at the position of the phase change hole 24, and the initial position of the phase change ball 23 is located closer to the solution. Therefore, the heat absorption efficiency of the phase change ball 23 is also greatly enhanced. The heat absorption of the storage tank 20 itself is also greatly enhanced. When the phase change ball 23 is at the position of the phase change hole 24, the phase change ball 23 begins to release heat rapidly, that is, to heat the detergent in the storage tank 20. Preheating the detergent to a suitable temperature can improve the activity of the detergent, accelerate the dissolution and shedding of dirt, and thus shorten the cleaning time.

[0028] Reference Figure 2-Figure 5The power unit includes a first pipe 25, a second pipe 26, a third pipe 27, an elastic rod 28 and a push rod 29. The bottom end of the first pipe 25 is closed. The elastic rod 28 is elastically arranged at the bottom end of the first pipe 25. The second pipe 26 is arranged in an arc shape and one end is connected to one end of the first pipe 25, and the other end is directly opposite to the opening of the phase change hole 24. One end of the third pipe 27 is directly opposite to the phase change hole 24, and the other end is connected to the bottom end of the first pipe 25. The push rod 29 is slidably arranged on the storage tank 20. The first pipe 25 and the second pipe 2 6 is larger than the size of the phase change ball 23, and the upper side wall of the second pipe 26 and the upper side wall of the third pipe 27 near the phase change hole 24 extend toward the phase change hole 24 to prevent the phase change ball 23 from falling off the storage tank 20 after sliding out of the first pipe 25, so as to facilitate guiding the phase change ball 23 into the phase change hole 24; in this embodiment, the elastic rod 28 is driven by an electric method, and a power electric push rod 30 is fixedly connected to the position of the first pipe 25 near the elastic rod 28, and a power electromagnet 3 is fixedly connected to the output end of the power electric push rod 30 2. The power electromagnet 32 ​​absorbs the elastic rod 28. The outer wall of the elastic rod 28 is fixedly connected with a handle 31. The outer wall of the elastic rod 28 is sleeved with a power spring 33. The two ends of the power spring 33 are respectively fixedly connected with the handle 31 and the bottom end of the first pipe 25. The power electromagnet 32 ​​is used to absorb the elastic rod 28, and the power electric push rod 30 pulls the elastic rod 28, and the power spring 33 is stretched and deformed. When the power electromagnet 32 ​​is powered off, under the elastic force of the power spring 33, the elastic rod 28 moves the phase change ball 23 toward the second pipe 26, the phase change ball 23 falls into the phase change hole 24, and the pushing rod 29 moves in the horizontal direction. The other side of the storage tank 20 is fixedly connected with a pushing electric push rod, which is fixedly connected to the pushing electric push rod 29 and can push the phase change ball 23 out of the phase change hole 24. Since the extended parts of the second pipe 26 and the third pipe 27 restrict the phase change ball 23, after the phase change ball 23 falls into the third pipe 27 under the action of its gravity, the phase change ball 23 returns to the bottom end of the first pipe 25 through the third pipe 27.

[0029] Reference Figure 6-Figure 7The control mechanism includes a control frame 35 rotatably arranged outside the fermentation tube, a top box 36, a bottom box 37, a liquid level sensor 38, a regulating pipe 39 and a regulating component for controlling the flow in the regulating pipe 39, the regulating pipe 39 is set as a hose, the regulating pipe 39 is in a stretched state, the liquid level sensor 38 is installed in the bottom box 37, the top box 36 and the bottom box 37 are fixedly connected to the control frame 35, the regulating pipe 39 is used to connect the top box 36 and the bottom box 37, and the size of the top box 36 can be larger than that of the bottom box 37, because it can ensure that the bottom box 37 is filled with water; the regulating component includes an adjusting block 40 fixed to the control frame 35, an adjusting rod 41, an adjusting wheel 42 and a regulating component for controlling An adjusting member 43 controls the movement of the adjusting rod 41, and the adjusting block 40 is provided with a through hole 44 for the adjusting tube 39 to pass through. The adjusting block 40 is also provided with an adjusting slot 45, the adjusting slot 45 is inclined, and the through hole 44 is vertically arranged, the length direction of the through hole 44 is not horizontal and intersects with the length direction of the adjusting slot 45, and both side walls of the adjusting slot 45 are provided with inclined slots 46, and the inclined slots 46 are arranged parallel to the length direction of the adjusting slot 45, and the two ends of the adjusting rod 41 slide in the two inclined slots 46 respectively, and the adjusting wheel 42 is rotatably arranged outside the adjusting rod 41, and the adjusting wheel 42 is located in the adjusting slot 45, and when the adjusting rod 41 is located at the two ends of the inclined slot 46, the two ends of the adjusting tube 39 are maximized or minimized.

[0030] In this embodiment, when the top box 36 is located at the top, during the downward movement of the adjusting wheel 42 and the adjusting rod 41, the distance between the peripheral wall of the adjusting wheel 42 and the bottom of the adjusting groove 45 becomes smaller and smaller, that is, the adjusting wheel 42 squeezes the adjusting tube 39. When the adjusting wheel 42 is located at the lowest end of the adjusting groove 45, the adjusting tube 39 is completely squeezed and deformed. At this time, the adjusting tube 39 is blocked. In this embodiment, the adjusting member 43 is used to drive the adjusting rod 41. The transmission part includes an adjusting electric push rod, which is fixed on the adjusting block 40, and the adjusting electric push rod The movement direction is set along the length direction of the inclined groove 46, and the adjusting electric push rod is fixedly connected to either end of the adjusting rod 41, so the adjusting electric push rod can push the adjusting rod 41 to reciprocate along the inclined groove 46. At this time, the adjusting wheel 42 rolls under the friction with the adjusting tube 39 and moves with the adjusting rod 41 to achieve the control effect of the flow rate of the adjusting tube 39; in the initial state, the adjusting electric push rod pushes the adjusting rod 41 to the lowest end of the inclined groove 46. At this time, the adjusting tube 39 is blocked. When the pH is adjusted in the fermenter 1 in the early stage, the measuring time needs to be shortened. time interval, so it is necessary to accelerate the cleaning effect of the pH sensor 5. At this time, the adjusting rod 41 needs to be pulled to the top of the adjusting groove 45. At this time, the adjusting tube 39 is in a fully open state. In this embodiment, this time can be set as the time for cleaning the pH sensor 5 with clean water. When the liquid level sensor 38 detects that the water level in the bottom box 37 rises to the top, the phase change ball 23 is immediately controlled to move into the phase change hole 24. The detergent in the storage tank 20 is heated, and the accelerating tube 21 is controlled to squeeze the detergent into the storage tank 20. At this time, through two wipes The repeated stirring of the wipe 9 greatly accelerates the mixing effect of the detergent, thereby greatly accelerating the efficiency of cleaning the pH sensor 5, and the water and detergent mixture after cleaning can be pumped away, and the next detection task can be started; finally, it is also necessary to swap the position of the top box 36 and the bottom box 37, and adjust the flow of the pipe 39 to fully open, so as to quickly flow the water in the bottom box 37 into the top box 36, and time the next cleaning work. In the middle and late stages of pH adjustment in the fermenter 1, it is only necessary to move the adjustment rod 41 and the adjustment wheel 42 to the middle position of the adjustment groove 45.

[0031] The implementation principle of the preparation device of an avermectin Streptomyces-Purpleurosporium lilacinum composite bacterial agent in the embodiment of the present application is as follows: after the driving motor 16 controls the driving gear 17 to rotate, the driving gear 17 controls the ring gear 18 to rotate, and the ring gear 18 drives the protective tube 4 to rotate, so as to achieve the rotation effect of the protective tube 4, and the composite bacterial agent is started to be used, and the composite bacterial agent is detected by the pH sensor 5 until the pH value meets the requirements; after the detection is completed, the pH sensor 5 needs to be cleaned, and water is injected into the detection tube 3, and the liquid surface of the water does not need to completely submerge the pH sensor 5. The wiping member 9 can absorb water, and the wiping member 9 impacts the pH sensor 5 through the reciprocating motion of the wiping member 9. In this embodiment, the distance between the wiping member 9 and the pH sensor 5 is short and due to the water resistance, the impact force of the wiping member 9 on the pH sensor 5 is small, and the pH sensor 5 will not be damaged. The wiping member 9 drives the water to fluctuate, and as the wiping member 9 and the pH sensor 5 are impacted and wiped and the water fluctuates and impacts, the solution adhering to the pH sensor 5 is greatly accelerated to fall off.

[0032] In order to accelerate the cleaning efficiency, the power electromagnet 32 ​​is used to absorb the elastic rod 28, and the elastic rod 28 is pulled by the power electric push rod 30, and the power spring 33 is stretched and deformed. When the power electromagnet 32 ​​is powered off, under the elastic force of the power spring 33, the elastic rod 28 moves the phase change ball 23 toward the second pipe 26, and the phase change ball 23 falls into the phase change hole 24. The phase change ball 23 begins to release heat rapidly, that is, to heat the detergent in the storage tank 20. Preheating the detergent to a suitable temperature can improve the activity of the detergent, accelerate the dissolution and shedding of dirt, thereby shortening the cleaning time. Through repeated stirring of the two wiping members 9, the mixing effect of the detergent is greatly accelerated, thereby greatly accelerating the cleaning time of the pH sensor 5, and the water and detergent mixture after cleaning can be pumped away, and the next detection task can be started.

[0033] In the early stage of pH adjustment in the fermentation tank 1, it is necessary to shorten the measurement time interval, so it is necessary to accelerate the cleaning effect of the pH sensor 5. At this time, it is necessary to pull the adjustment rod 41 to the top of the adjustment tank 45. At this time, the adjustment tube 39 is in a fully open state. In this embodiment, this time can be set as the time for cleaning the pH sensor 5 with clean water. When the liquid level sensor 38 detects that the water level in the bottom box 37 rises to the top, the phase change ball 23 is immediately controlled to move into the phase change hole 24. The detergent in the storage tank 20 is heated. Through repeated stirring of the two wiping members 9, the mixing effect of the detergent is greatly accelerated, and the efficiency of cleaning the pH sensor 5 is greatly accelerated. Finally, it is also necessary to swap the position of the top box 36 and the bottom box 37, and adjust the flow of the adjustment tube 39 to fully open the flow, and quickly flow the water in the bottom box 37 into the top box 36 to time the next cleaning work. In the middle and late stages of pH adjustment in the fermentation tank 1, it is only necessary to move the adjustment rod 41 and the adjustment wheel 42 to the middle position of the adjustment tank 45.

[0034] Embodiment 2

[0035] The present application example discloses a method for preparing a composite bacterial agent of Streptomyces avermectinus and Pseudomonas lilacinus. Figure 1 The preparation method of the composite bacterial agent of Streptomyces avermectin and Pseudomonas erythrorhizium comprises the following steps: S1. Preparation of bacterial strains: Take out Streptomyces avermectin, Pseudomonas lilacinus, and Brevibacillus laterosporus to ensure that the strains have good activity; ensure the purity and activity of Streptomyces avermectin, perform rejuvenation and screening when necessary, check the biological characteristics and functions of Pseudomonas lilacinus to ensure that it has the expected control effect, obtain the strain of Brevibacillus laterosporus, and ensure that it has good growth and reproduction ability; S2. Culture medium configuration: optimize the formula and conditions of the culture medium through experiments to improve the yield and activity of the composite bacteria; configure a basic culture medium suitable for the co-growth of three microorganisms. The culture medium should contain appropriate amounts of carbon source, nitrogen source, inorganic salts and necessary trace elements, and can be appropriately adjusted according to the nutritional requirements of each strain to achieve the best composite fermentation effect. Considering the demand of Streptomyces avermectin for the synthesis of avermectin, specific precursors or inducers can be added to the culture medium; S3: Composite fermentation: Inoculation: Inoculate the prepared Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus strains into the basal medium in a certain proportion; the inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure that they can grow in a coordinated manner during the composite fermentation process; S4: Fermentation condition control: Control the temperature, pH value, dissolved oxygen content and other parameters during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; the detection mechanism is used to monitor and control the pH value in real time; during the fermentation process, the composite bacterial agent is randomly inspected and checked at any time through the detection tube 3, and the pH value of the composite bacterial agent is adjusted in real time to ensure the fermentation conditions. In addition, it is also necessary to combine the fermentation frequently and intelligently adjust the time interval for pH detection to reasonably apply monitoring methods, save costs to the greatest extent, and achieve the best pH adjustment effect.

[0036] S5: Separation and purification: After fermentation, the bacteria and fermentation liquid are separated by centrifugation, filtration and other methods to obtain the composite bacterial agent. The composite bacterial agent is subjected to biological activity determination and safety evaluation to ensure its effectiveness and safety in practical applications, and is promoted and applied in agriculture, horticulture and other fields to exert its broad-spectrum, efficient, safe and long-term biological control effect.

[0037] Embodiment 3

[0038] The present application example discloses a composite bacterial agent of Streptomyces avermectinus and Pseudomonas lilacinus. Figure 1 The composite microbial agent of Streptomyces avermectin and Pseudomonas lilacinus includes Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus. When necessary, other adjuvants are also needed to adjust the biological properties of the composite microbial agent. The composite bacteria combines the respective advantages of the three microorganisms and can more widely control a variety of pests, nematodes and pathogens. There may be synergistic effects between different microorganisms to jointly improve the control effect. For example, Pseudomonas lilacinus and Brevibacillus laterosporus may jointly inhibit the growth of nematodes and pathogens through different mechanisms. The use of composite bacteria can reduce the drug resistance problem caused by the continuous use of single microbial preparations. Finally, Pseudomonas lilacinus and Brevibacillus laterosporus can produce physiologically active substances that promote plant growth. The use of composite bacteria may more significantly promote plant growth and development. The composite bacteria of Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus have broad application prospects and significant advantages in agriculture.

[0039] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A preparation device for a composite bacterial agent of Streptomyces avermectinus and Pseudomonas erythrorhizium, characterized in that: It comprises a strain preparation module, a culture medium configuration module, a composite fermentation module and a centrifugal purification module, wherein the composite fermentation module comprises a fermentation tank (1), a detection mechanism for detecting the composite bacterial agent, a control mechanism for controlling the measurement time interval and a cleaning mechanism; The detection mechanism comprises a detection frame (2) which is lifted and arranged in the fermentation tank (1), a plurality of detection cylinders (3) fixed on the detection frame (2), a protective cylinder (4) which is rotatably arranged outside the detection cylinder (3), a pH sensor (5) arranged in the detection cylinder (3), and a driving component for controlling the rotation of the protective cylinder (4); the detection cylinder (3) is provided with a detection hole (6), the protective cylinder (4) is provided with a protection hole (7), and when the protective cylinder (4) is in an initial position, the inner wall of the protective cylinder (4) blocks the detection hole (6); and the cleaning mechanism is used for cleaning the pH sensor (5).

2. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 1, characterized in that: The cleaning mechanism comprises a cleaning rack (8) which is lifted and lowered in the protective cylinder (4), a cleaning component for cleaning the pH sensor (5), an accelerating component for accelerating the cleaning of the pH sensor (5), and a circulation component for realizing water circulation in the detection cylinder (3).

3. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 2, characterized in that: The cleaning assembly comprises two wiping members (9) elastically arranged on the cleaning frame (8) and a driving unit for realizing elastic movement of the two wiping members (9), and the two wiping members (9) are respectively located on both sides of the pH sensor (5).

4. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 5, characterized in that: The driving part comprises two slide plates (10), two first springs (11), two first electromagnets (12) and two driving members (34); the wiping member (9) is fixed on the slide plates (10); the two first springs (11) are used to realize elastic arrangement of the two slide plates (10) on the cleaning rack (8); the driving member (34) is used to control the reciprocating motion of the first electromagnet (12); and the first electromagnet (12) is used to adsorb the slide plates (10).

5. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 4, characterized in that: The acceleration assembly comprises a storage tank (20), an acceleration tube (21), a solenoid valve (22), a phase change ball (23) and a power unit for driving the phase change ball (23) to move. The storage tank (20) is mounted on the inner wall of the fermentation tank (1). One end of the acceleration tube (21) is connected to the storage tank (20), and the other end is connected to the detection cylinder (3). The storage tank (20) is filled with a cleaning agent for cleaning a pH sensor (5). The storage tank (20) is provided with a phase change hole (24), and the phase change hole (24) is used to place the phase change ball (23). The phase change ball (23) is filled with a phase change material.

6. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 5, characterized in that: The power unit comprises a first pipe (25), a second pipe (26), a third pipe (27), an elastic rod (28) and a push rod (29); the bottom end of the first pipe (25) is closed; the elastic rod (28) is elastically arranged at the bottom end of the first pipe (25); the second pipe (26) is arranged in an arc shape and one end is connected to one end of the first pipe (25), and the other end is opposite to the opening of the phase change hole (24); one end of the third pipe (27) is opposite to the phase change hole (24), and the other end is connected to the bottom end of the first pipe (25); the push rod (29) is slidably arranged on the storage tank (20).

7. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 4, characterized in that: The control mechanism comprises a control frame (35) rotatably arranged outside the fermentation tube, a top box (36), a bottom box (37), a liquid level sensor (38), a regulating pipe (39) and a regulating component for controlling the flow in the regulating pipe (39), wherein the regulating pipe (39) is arranged as a hose, and the regulating pipe (39) is in a stretched state, the liquid level sensor (38) is installed in the bottom box (37), the top box (36) and the bottom box (37) are fixedly connected to the control frame (35), and the regulating pipe (39) is used to connect the top box (36) and the bottom box (37).

8. The preparation device of the avermectin Streptomyces-Purpurosporium lilacinum composite bacterial agent according to claim 7, characterized in that: The regulating assembly comprises a regulating block (40) fixed on the control frame (35), a regulating rod (41), a regulating wheel (42) and a regulating member (43) for controlling the movement of the regulating rod (41); the regulating block (40) is provided with a through hole (44) for the regulating tube (39) to pass through; the regulating block (40) is also provided with a regulating groove (45); the regulating groove (45) is inclined; both side walls of the regulating groove (45) are provided with inclined grooves (46); the two ends of the regulating rod (41) slide in the two inclined grooves (46) respectively; the regulating wheel (42) is rotatably arranged outside the regulating rod (41) and is located in the regulating groove (45); when the regulating rod (41) is located at the two ends of the inclined groove (46), the flow rate of the regulating tube (39) is maximum or minimum.

9. A method for preparing a composite bacterial agent of Streptomyces avermectinus and Pseudomonas erythrorhizium, characterized in that: The steps include: S1. Preparation of bacterial strains: Take out Streptomyces avermectin, Pseudomonas lilacinus, and Brevibacillus laterosporus to ensure that the bacterial strains have good activity; S2. Culture medium configuration: Optimize the culture medium formula and conditions through experiments to increase the yield and activity of the composite bacteria; S3: composite fermentation: inoculation: inoculating the prepared Streptomyces avermectin, Pseudomonas lilacinus and Brevibacillus laterosporus strains into the basal medium in a certain proportion; S4: Fermentation condition control: Control the temperature, pH value, dissolved oxygen content and other parameters during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; the detection mechanism is used to monitor and control the pH value in real time; S5: Separation and purification: After the fermentation is completed, the bacteria and the fermentation liquid are separated by centrifugation, filtration and other methods to obtain the composite bacterial agent.

10. An avermectin Streptomyces-Purpleurosporium lilacinum composite bacterial agent, prepared by the preparation method of the avermectin Streptomyces-Purpleurosporium lilacinum composite bacterial agent according to any one of claims 9, characterized in that: These include Streptomyces avermectinus, Bacillus lilacinus, and Brevibacillus laterosporus.

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