Animal material decellularization system and use method

Abstract

The invention relates to the technical field of decellularization systems, and discloses an animal material decellularization system and a use method.The animal material decellularization system comprises a tank body, a liquid inlet pipe is installed at the top of the tank body, and a liquid outlet pipe is installed at the bottom of the tank body of a decellularization cavity; a decellularization treatment module is arranged in a tank body area between the liquid inlet pipe and the liquid outlet pipe; the decellularization treatment module comprises a frequency-adjustable ultrasonic generator and a vibrating screen and is used for providing physical stimulation with different intensities required by decellularization treatment. Through the arrangement of the decellularization processing module comprising the frequency-adjustable ultrasonic generator and the vibrating screen, a user can select ultrasonic waves with different frequencies according to the characteristics of cell materials, and the decellularization requirements of different types of animal materials are met; by arranging the temperature control module, the animal material decellularization system can adjust the temperature in real time, and the problems that in the decellularization process in the prior art, the temperature is inconvenient to monitor, the decellularization efficiency is low, and the decellularization quality is poor are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of decellularization systems, and in particular to an animal material decellularization system and a use method thereof. Background Art

[0002] Decellularization technology includes physical and chemical methods, which can destroy cells in tissues through surfactant infusion to achieve the effect of removing cells. A good decellularized scaffold should remove the immunogenic components, completely retain the extracellular matrix and microstructure, retain biological activity and good mechanical properties, and can be used as a scaffold for culturing cells. Traditional decellularization methods are mostly manual operations. The temperature during the decellularization process is not easy to monitor, the decellularization efficiency is low and the quality is poor, and it is difficult to ensure the consistency of the decellularization effect. In addition, manual operations may also introduce contamination, affecting the subsequent application of biomaterials. Summary of the invention

[0003] The present invention aims to solve one of the technical problems in the above-mentioned related art at least to a certain extent.

[0004] In order to achieve the above-mentioned purpose, one aspect of the present invention discloses an animal material decellularization system, including a tank body, a liquid inlet pipe is installed on the top of the tank body, a liquid outlet pipe is installed on the bottom of the decellularization chamber tank body, and the tank body area between the liquid inlet pipe and the liquid outlet pipe is provided with: a decellularization treatment module: including an ultrasonic generator and a vibrating screen with adjustable frequency, which are used to provide physical stimulation of different intensities required for decellularization treatment; a temperature control module: including a temperature sensor, a heating element and a cooling element, wherein the temperature sensor is connected to the heating element, and the temperature sensor is connected to the cooling element by signal, so as to accurately control the temperature of the area at the decellularization treatment module; a cleaning module: including at least two cleaning tanks with different chemical properties and a circulating filtration pipeline, which are used to thoroughly clean the decellularized material; a drying module: including a hot air generator and a circulating fan, which are used to evenly dry the material; a quality monitoring module: the quality monitoring module includes an optical sensor and a biosensor, which are used to monitor the decellularization degree and biocompatibility of the material; an integrated control module, including at least one touch screen operation interface and a central processing unit, which are used to monitor and adjust the entire decellularization process in real time.

[0005] In addition, the animal material decellularization system according to the above embodiment of the present invention may also have the following additional technical features:

[0006] Furthermore, a waste liquid recovery box is provided at the bottom of the tank body, and the waste liquid recovery box is connected to the liquid outlet pipe.

[0007] Furthermore, the heating element and the cooling element of the temperature control module can be independently controlled to achieve precise temperature regulation.

[0008] Furthermore, the circulating filtration pipeline of the cleaning module includes at least one microfilter and one ultrafilter, which are used to remove particles and macromolecules in the cleaning liquid.

[0009] Furthermore, the drying module is also provided with a temperature sensor, and the temperature sensor is arranged on the hot air generator to ensure the uniformity of the hot air temperature.

[0010] Furthermore, the touch screen operation interface of the integrated control module includes at least one graphical user interface for simplifying the operation process and providing real-time feedback.

[0011] Furthermore, a syringe pump is installed on the tank body, and the syringe pump is connected to the liquid inlet pipe.

[0012] Furthermore, the circulating filtering pipeline adopts a meandering pipeline, and the liquid outlet end of the filtering pipeline is detachably connected with a filter screen.

[0013] According to the animal material decellularization system of the present invention, a decellularization processing module including an ultrasonic generator with adjustable frequency and a vibrating screen is provided, so that the user can select ultrasonic waves of different frequencies according to the characteristics of the cell material to meet the decellularization requirements of different types of animal materials; the temperature control module is provided, so that the animal material decellularization system can adjust the temperature in real time to ensure that the decellularization process is carried out at the optimal temperature, wherein the temperature sensor is used to monitor the temperature of the decellularization area in real time, thereby avoiding the adverse effects caused by temperature changes during the decellularization process; two cleaning tanks with different chemical properties are provided to remove residues on the surface of the decellularized material, thereby ensuring that the surface of the decellularized material is clean; the drying module is provided, so that the decellularized material can be conveniently dried in the tank, reducing the possibility of external contamination during the transfer process; the quality monitoring module is provided, so that the user can monitor the material changes in the decellularization process in real time through the quality monitoring module, thereby facilitating corresponding operations.

[0014] On the other hand, the present invention discloses a method for using an animal material decellularization system, comprising the following steps: S1, placing the animal material to be processed into a tank body; S2, adding a preset proportion of chemical reagents through a liquid inlet pipe; S3, conveying the material to a decellularization processing module, and selecting ultrasonic and vibrating screen vibration for processing according to the material properties; S4, adjusting the temperature of the processing area in real time through a temperature control module to adapt to the processing requirements of different stages; S5, sending the physically treated material to a cleaning module for gradual cleaning; S6, sending the cleaned material to a drying module for uniform drying; S7, using a quality monitoring module to evaluate the biocompatibility and decellularization degree of the dried material.

[0015] Furthermore, the method also includes recording the parameters of the entire decellularization process through an integrated control module, and performing real-time monitoring and adjustment through a touch screen operation interface.

[0016] According to the method of using the animal material decellularization system of the present invention, direct manual contact operations are reduced throughout the entire decellularization process, thereby improving efficiency and repeatability; through the integrated control module, the proportion of chemical reagents, the intensity of ultrasound and vibration screen, and the temperature of the temperature control module can be accurately controlled to ensure the consistency and predictability of the decellularization process, and the processing parameters of ultrasound and vibration screen can be adjusted according to the characteristics of different animal materials, providing a high degree of adaptability and flexibility; through the set touch screen operation interface, the operator can monitor the decellularization process in real time and make adjustments as needed, thereby improving the responsiveness and controllability of the system.

[0017] The entire animal material decellularization system includes multiple stages such as physical treatment, temperature control, cleaning, drying and quality monitoring to ensure that the materials are fully and thoroughly processed. The multi-stage cleaning and circulating filtration pipelines of the cleaning module can completely remove the residues and contaminants on the surface of the material and improve the biosafety of the material; the quality monitoring module can evaluate the biocompatibility and decellularization degree of the dried material to ensure that the material meets the expected quality standards.

[0018] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0020] Figure 1 is a cross-sectional view of the animal material decellularization system of the present invention;

[0021] Figure 2 is a front view of the animal material decellularization system of the present invention;

[0022] Figure 3 It is a schematic diagram of the cleaning tank structure of the animal material decellularization system of the present invention;

[0023] Figure 4 It is a schematic diagram of the circulation filtration pipeline structure of the animal material decellularization system of the present invention.

[0024] In the figure: 1. tank body; 2. liquid inlet pipe; 3. injection pump; 4. liquid outlet pipe; 5. waste liquid recovery box; 6. ultrasonic generator; 7. vibrating screen; 8. temperature sensor; 9. heating element; 10. cooling element; 11. microfilter; 12. ultrafilter; 13. cleaning tank; 14. circulating filtration pipeline; 15. filter screen; 16. hot air generator; 17. circulating fan; 18. touch screen; 19. circulating pump. DETAILED DESCRIPTION

[0025] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0026] like Figures 1 to 4 As shown, the animal material decellularization system according to one embodiment of the present invention includes a tank body 1, a liquid inlet pipe 2 is installed on the top of the tank body 1, and an injection pump 3 is installed on the tank body 1, and the injection pump 3 is connected to the liquid inlet pipe 2. When the system is started, the liquid inlet pipe 2 first introduces an appropriate amount of chemical reagent into the tank body 1. By setting the injection pump 3, the amount of chemical reagent can be accurately controlled and added, thereby ensuring the smooth progress of the decellularization process. A liquid outlet pipe 4 is installed at the bottom of the decellularization chamber tank body 1, and a waste liquid recovery box 5 is set at the bottom of the tank body 1, and the waste liquid recovery box 5 is connected to the liquid outlet pipe 4. By setting the waste liquid recovery box 5, the waste liquid generated in the decellularization process can be centrally recovered and processed, reducing the possibility of pollution caused by leakage of chemical reagents.

[0027] The tank 1 area between the liquid inlet pipe 2 and the liquid outlet pipe 4 is provided with: a decellularization treatment module: including an ultrasonic generator 6 with adjustable frequency and a vibrating screen 7, which are used to provide physical stimulation of different intensities required for decellularization treatment. The vibrating screen 7 is located at the bottom of the ultrasonic generator 6. By setting the decellularization treatment module including the ultrasonic generator 6 with adjustable frequency and the vibrating screen 7, the user can select ultrasonic waves of different frequencies according to the characteristics of the cell material to meet the decellularization requirements of different types of animal materials. By setting the vibrating screen 7, the decellularization treatment module can provide additional physical stimulation, enhance the decellularization effect, and then better physically stimulate the animal material.

[0028] Temperature control module: includes temperature sensor 8, heating element 9 and cooling element 10, which are used to accurately control the temperature of the area at the decellularization treatment module. Specifically, the temperature sensor 8 is connected to the heating element 9, and the temperature sensor 8 is connected to the cooling element 10 by signal. The heating element 9 and the cooling element 10 of the temperature control module can be controlled independently to achieve precise temperature regulation. Through the set temperature control module, the animal material decellularization system can adjust the temperature in real time to ensure that the decellularization process is carried out at the optimal temperature, wherein the temperature sensor 8 is used to monitor the temperature of the decellularization area in real time. The heating element 9 is used to quickly increase the temperature when needed, and an electric heating wire heating plate or the like can be used. The cooling element 10 is used to maintain a constant low-temperature environment to protect the material from thermal damage, and the cooling element 10 uses a semiconductor refrigeration sheet.

[0029] Cleaning module: includes at least two cleaning tanks 13 with different chemical properties and a circulating filtration pipeline 14, which are used to thoroughly clean the decellularized material; the circulating filtration pipeline 14 of the cleaning module includes at least one microfilter 11 and an ultrafilter 12, which are used to remove particles and macromolecules in the cleaning liquid, ensure the cleanliness of the cleaning liquid, and avoid cross contamination. The ultrafilter 12 can remove smaller particles and molecules, such as proteins and peptides, to further purify the material, and the microfilter 11 can remove larger cell fragments and microorganisms to ensure that the surface of the material is clean and free of biological contamination. The cleaning liquid of the cleaning module is circulated through a circulating pump 19. The two cleaning tanks 13 with different chemical properties are used to remove residues on the surface of the material after decellularization. The circulating filtration pipeline 14 adopts a zigzag pipeline, and the liquid outlet end of the filtration pipeline is detachably connected with a filter 15. During the decellularization process, the cleaning module automatically starts after the decellularization treatment, and the cleaning liquid in the cleaning tank 13 is continuously updated through the circulating filtration pipeline 14 to maintain the cleaning effect. The detachably connected filter screen 15 can be easily cleaned in time, thus avoiding the problem of the filter screen 15 being clogged due to excessive debris adhering to it and reducing the cleaning effect.

[0030] Drying module: includes a hot air generator 16 and a circulating fan 17. The hot air generator 16 is used to generate evenly distributed hot air to accelerate the evaporation of moisture on the surface of the material. The circulating fan 17 is used to ensure that the hot air flows evenly in the tank body 1 and evenly dries the material. The drying module is also provided with a temperature sensor 8, which is arranged on the hot air generator 16 to ensure the uniformity of the hot air temperature.

[0031] Quality monitoring module: The quality monitoring module includes an optical sensor and a biosensor, which are used to monitor the decellularization degree and biocompatibility of the material; specifically, the optical sensor is a fluorescence sensor, and the biosensor is a cell sensor. The quality monitoring module is set up so that the user can monitor the material changes in the decellularization process in real time through the quality monitoring module, which is conducive to making corresponding operations.

[0032] The integrated control module includes at least one touch screen 18 operation interface and a central processing unit for real-time monitoring and adjustment of the entire decellularization process. The touch screen 18 operation interface of the integrated control module includes at least one graphical user interface for simplifying the operation process and providing real-time feedback.

[0033] The present invention also provides a method for using an animal material decellularization system, comprising the following steps: S1, placing the animal material to be processed into a tank body 1; S2, adding a preset proportion of chemical reagents through a liquid inlet pipe 2; S3, conveying the material to a decellularization processing module, and selecting ultrasonic waves and a vibrating screen 7 for vibration processing according to the material properties; S4, adjusting the temperature of the processing area in real time through a temperature control module to adapt to the processing requirements of different stages; S5, sending the physically treated material to a cleaning module for step-by-step cleaning; S6, sending the cleaned material to a drying module for uniform drying; S7, using a quality monitoring module to evaluate the biocompatibility and decellularization degree of the dried material.

[0034] In one embodiment, the integrated control module is used to record the parameters of the entire decellularization process, and the touch screen 18 operation interface is used to perform real-time monitoring and adjustment.

[0035] When using the animal material decellularization system of the present invention, the efficiency and repeatability are improved by reducing direct manual contact operations throughout the entire decellularization process; through the integrated control module, the proportion of chemical reagents, the intensity of ultrasound and vibration screen 7, and the temperature of the temperature control module can be accurately controlled to ensure the consistency and predictability of the decellularization process, and the processing parameters of ultrasound and vibration screen 7 can be adjusted according to the characteristics of different animal materials, providing a high degree of adaptability and flexibility; through the set touch screen 18 operation interface, the operator can monitor the decellularization process in real time and make adjustments as needed, thereby improving the responsiveness and controllability of the system.

[0036] The entire animal material decellularization system includes multiple stages such as physical treatment, temperature control, cleaning, drying and quality monitoring to ensure that the material is fully and thoroughly processed. The multi-stage cleaning and circulating filtration pipeline 14 of the cleaning module can completely remove the residues and contaminants on the surface of the material, thereby improving the biosafety of the material; the biocompatibility and decellularization degree of the dried material can be evaluated through the quality monitoring module to ensure that the material meets the expected quality standards; the parameters of the entire decellularization process can be recorded through the integrated control module, providing a basis for subsequent data analysis and process optimization.

[0037] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0038] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0039] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A system for decellularizing animal materials, characterized in that: The tank body (1) comprises a liquid inlet pipe (2) installed on the top of the tank body (1), a liquid outlet pipe (4) installed on the bottom of the decellularization chamber tank body (1), and the tank body (1) region between the liquid inlet pipe (2) and the liquid outlet pipe (4) is provided with: The decellularization treatment module includes an ultrasonic generator (6) with adjustable frequency and a vibrating screen (7), which are used to provide physical stimulations of different intensities required for the decellularization treatment; The temperature control module comprises a temperature sensor (8), a heating element (9) and a cooling element (10), wherein the temperature sensor (8) is connected to the heating element (9), and the temperature sensor (8) is connected to the cooling element (10) by signals, and is used to accurately control the temperature of the area at the decellularization treatment module; The cleaning module comprises at least two cleaning tanks (13) of different chemical properties and a circulating filtration pipeline (14), which are used to thoroughly clean the decellularized material; Drying module: comprising a hot air generator (16) and a circulation fan (17), for uniformly drying the material; Quality monitoring module: The quality monitoring module includes optical sensors and biosensors for monitoring the degree of decellularization and biocompatibility of the material; The integrated control module includes at least one touch screen (18) operation interface and a central processing unit for real-time monitoring and adjustment of the entire decellularization process.

2. The animal material decellularization system according to claim 1, characterized in that: A waste liquid recovery box (5) is provided at the bottom of the tank body (1), and the waste liquid recovery box (5) is connected to the liquid outlet pipe (4).

3. The animal material decellularization system according to claim 1, characterized in that: The heating element (9) and the cooling element (10) of the temperature control module can be independently controlled to achieve precise temperature regulation.

4. The animal material decellularization system according to claim 1, characterized in that: The circulating filtration pipeline (14) of the cleaning module comprises at least one microfilter (11) and an ultrafilter (12), which are used to remove particles and macromolecules in the cleaning liquid.

5. The animal material decellularization system according to claim 1, characterized in that: The drying module is also provided with a temperature sensor (8), and the temperature sensor (8) is arranged on the hot air generator (16) to ensure the uniformity of the hot air temperature.

6. The animal material decellularization system according to claim 1, characterized in that: The touch screen (18) operating interface of the integrated control module includes at least one graphical user interface for simplifying the operating process and providing real-time feedback.

7. The animal material decellularization system according to claim 1, characterized in that: An injection pump (3) is installed on the tank body (1), and the injection pump (3) is connected to the liquid inlet pipe (2).

8. The animal material decellularization system according to claim 1, characterized in that: The circulating filtering pipeline (14) is a meandering pipeline, and a filter screen (15) is detachably connected to the liquid outlet end of the filtering pipeline.

9. A decellularization method using the animal material decellularization system according to any one of claims 1 to 8, characterized in that: The following steps are included: S1. placing the animal material to be processed into a tank (1); S2, adding a chemical reagent in a preset proportion through the liquid inlet pipe (2); S3, transporting the material to a decellularization treatment module, and selecting ultrasound and a vibrating screen (7) for vibration treatment according to the material characteristics; S4. The temperature of the processing area is adjusted in real time through the temperature control module to adapt to the processing requirements at different stages; S5, sending the physically treated material into a cleaning module for step-by-step cleaning; S6, sending the cleaned material into the drying module for uniform drying; S7. Use the quality monitoring module to evaluate the biocompatibility and decellularization degree of the dried material.

10. A method for using an animal material decellularization system, characterized in that: It also includes recording the parameters of the entire decellularization process through an integrated control module, and performing real-time monitoring and adjustment through a touch screen (18) operating interface.

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