Colla corii asini for protecting female ovary and mammary gland functions and preparation method thereof

Abstract

The invention belongs to the technical field of colla corii asini products, and particularly relates to colla corii asini capable of protecting female ovary and mammary gland functions and a preparation method of the colla corii asini. The donkey hide is subjected to a refining process to obtain concentrated donkey-hide gelatin, then the sea-buckthorn extract and the ginkgo leaf extract are added to prepare the donkey-hide gelatin block, and the donkey-hide gelatin block has the functions of protecting female ovaries and mammary glands and also has excellent color and luster and light transmission.

Description

Technical Field

[0001] The invention belongs to the technical field of donkey-hide gelatin products, and in particular relates to donkey-hide gelatin for protecting female ovary and breast functions and a preparation method thereof. Background Art

[0002] Premature ovarian failure generally refers to the condition in which women experience persistent amenorrhea before reaching menopause. In today's society, about 1% of women will have corresponding disease problems. POF usually manifests as disordered sex hormone levels, hot flashes and ovarian tissue atrophy, which causes female infertility and seriously affects the quality of life of patients.

[0003] Estrogen is an important sex hormone mainly secreted by the ovaries. It acts on multiple organs of the body, not only affecting the growth, differentiation and function of organs such as the ovaries, uterus and breasts, but also exerting effects on the nervous system, cardiovascular system and bone tissue. Imbalanced expression of estrogen receptors can cause a variety of diseases. Epidemiological surveys have found that an increase in endogenous estrogen, the use of exogenous estrogen during hormone replacement therapy and long-term exposure to estrogen are all associated with an increased risk of breast cancer.

[0004] Currently, antibiotics or hormone replacement therapy are mostly used in clinical treatment of premature ovarian failure and estrogen metabolism diseases, and the effect is remarkable. However, long-term use of antibiotics may cause biological antagonism and even induce the growth of estrogen-related tumor cells.

[0005] Donkey hide gelatin is a solid glue made by boiling and concentrating the dried or fresh skin of donkeys, an equine animal. It mainly contains collagen, gelatin, protein, and various amino acids such as L-hydroxyproline, glycine, alanine, glutamic acid, and contains various metal elements such as potassium, sodium, and calcium. It can be used to nourish blood and yin, moisturize dryness, and stop bleeding. It is deeply loved by consumers for its excellent nourishing and health-preserving effects.

[0006] Xanthoceras sorbifolia oil is a mixture of more than 90 substances in 14 categories. It is easily absorbed and not easily oxidized. It is deposited in the walls of human blood vessels and coronary arteries, making it an ideal edible oil with longevity and health functions. It is known as longevity oil. Relevant research experts pointed out that Xanthoceras sorbifolia has strong anti-cancer activity and has high inhibitory activity against a variety of human tumor cells (including breast cancer, prostate cancer, gastric cancer, liver cancer, cervical cancer, and leukemia).

[0007] Therefore, by adding natural active ingredients to donkey-hide gelatin, developing a donkey-hide gelatin that protects women's ovarian and breast functions and a preparation method thereof, the application prospects of donkey-hide gelatin can be further improved. Summary of the invention

[0008] In order to solve the above technical problems, a kind of donkey-hide gelatin for protecting female ovarian and breast function and its preparation method are disclosed. Donkey-hide gelatin tablets are prepared by multiple refining processes. By adding rock sugar, Xanthoceras sorbifolia oil, rice wine, sea buckthorn extract and ginkgo leaf extract into the preparation process of donkey-hide gelatin, the sensory properties of donkey-hide gelatin are improved and the application prospects of donkey-hide gelatin are expanded.

[0009] In order to achieve the above object, the present invention adopts the following technical solutions:

[0010] A donkey-hide gelatin for protecting female ovarian and breast functions. The donkey-hide gelatin raw materials mainly include donkey skin, crystal sugar, Xanthoceras sorbifolia oil, yellow rice wine, sea buckthorn extract, and ginkgo leaf extract.

[0011] Preferably, the donkey-hide gelatin raw material mainly includes 80-100 parts of donkey hide, 2-3 parts of crystal sugar, 2-3 parts of Xanthoceras sorbifolia oil, 0.5-1 part of rice wine, 0.1-1 part of sea buckthorn extract, and 0.1-1 part of ginkgo leaf extract in parts by mass.

[0012] Further preferably, the mass ratio of Xanthoceras chinensis oil: rock sugar: rice wine in the donkey-hide gelatin raw material is 0.8-1:1:0.2.

[0013] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0014] S1, selecting, cleaning, soaking, dehairing and defleshing, cutting, washing and blanching donkey skin to obtain blanched donkey skin;

[0015] S2, the blanched donkey skin of S1 is placed in an extraction device, water is added, heated and steam is introduced to extract twice, the glue is filtered to remove slag, and the glue obtained twice is combined to obtain the processed glue;

[0016] S3, the glue juice treated in S2 is transferred to a sandwich pot, boiled over high heat, then concentrated over low heat until the moisture content of the glue solution is 50-65%, add 20-25°C water, boil over high heat, then concentrate over low heat until the moisture content of the glue solution is 50-65%, then add 20-25°C water, boil again, and concentrate, repeat this process 9 times to obtain a purified glue solution;

[0017] S4, filtering the purified glue solution in S3 through 200 mesh and 800 mesh respectively while hot, transferring it into a sandwich pot, heating and concentrating it, and when the glue solution is concentrated to the point where hanging beads can be picked up with a rubber spatula, adding crystal sugar, Xanthoceras sorbifolia oil, rice wine, sea buckthorn extract, and ginkgo leaf extract in sequence, mixing them evenly, to obtain a mixed glue solution;

[0018] S5, concentrating the mixed glue solution of S4 to a hanging flag, and when the water content is 24-30wt%, discharging the glue, precooling, solidifying, and forming a glue block;

[0019] S6, drying, cutting and rubbing the rubber block obtained in S5 to obtain a film.

[0020] Preferably, the blanching of the skin in S1 is performed under steam pressure for 40 minutes at a ratio of skin weight to alkaline water weight of 1:0.5.

[0021] Preferably, the alkaline water is a 0.5% sodium carbonate aqueous solution with a vapor pressure value of 0.12-0.15 MPa.

[0022] Preferably, in S2, water is added according to a mass ratio of the donkey skin after blanching in S1 to water of 1:0.6.

[0023] Preferably, the water content in the purified glue solution described in S3 is 50-65wt%.

[0024] Preferably, the precooling temperature in S5 is 2-7°C, and the solidification temperature is 0-2°C.

[0025] Preferably, the drying temperature in S6 is 16-26° C., and the humidity is 55%-70%.

[0026] Preferably, the donkey-hide gelatin prepared above can be used in food, health products or medicines.

[0027] Beneficial Effects

[0028] 1. The present invention adds Xanthoceras sorbifolia oil, Hippophae rhamnoides extract and Ginkgo biloba leaf extract into the preparation process of donkey-hide gelatin, and finds that the donkey-hide gelatin prepared by compounding the three plant ingredients can play a role in protecting female ovaries and breasts.

[0029] 2. The present invention found that adding seabuckthorn extract and ginkgo leaf extract to the donkey-hide gelatin extracted after multiple refinings significantly improved the sensory qualities of the donkey-hide gelatin, such as color and light transmittance. DETAILED DESCRIPTION

[0030] The technical scheme of the present invention is further described below in conjunction with specific embodiments. In the following embodiments, seabuckthorn extract is purchased from Shaanxi Jinkangtai Biotechnology Co., Ltd., and ginkgo leaf extract is purchased from Shanghai Langzhi Biotechnology Co., Ltd. Unless otherwise specified, other raw materials can be obtained from conventional commercial channels; the processes used, unless otherwise specified, all adopt conventional processes in the art.

[0031] Example 1

[0032] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0033] S1, selecting, cleaning, soaking, dehairing, defleshing, cutting and washing the donkey skin to obtain strip donkey skin. According to 0.5 times of the weight of the donkey skin, adding a 0.5wt% sodium carbonate aqueous solution to the donkey skin, and blanching the skin for 40min while maintaining the steam pressure at 0.14Mpa, to obtain blanched donkey skin.

[0034] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0035] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65wt%, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 65wt%. Then, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 65wt%.

[0036] S4. Filter the glue liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of Xanthoceras sorbifolia oil, 1 part of rice wine, 1 part of sea buckthorn extract, and 1 part of ginkgo leaf extract in sequence, mix well, and obtain a mixed glue liquid.

[0037] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0038] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0039] Example 2

[0040] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0041] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0042] S2, 80 parts of blanched donkey skins of S1 are placed in an extraction device, 0.6 times the mass of water compared to the blanched donkey skins is added for heating and boiling, and steam is introduced to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. The glue is filtered and the residue is removed; then the above-mentioned process of adding water, heating and boiling, and introducing steam for extraction is repeated. After combining the glues extracted twice, the treated glue is obtained.

[0043] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 50%, add water (water temperature 20°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 50%. Then add water (water temperature 20°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 50wt%.

[0044] S4. Filter the glue liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 2 parts of crystal sugar, 2 parts of Xanthoceras sorbifolia oil, 0.5 parts of rice wine, 0.1 parts of sea buckthorn extract, and 0.1 parts of ginkgo leaf extract in turn, mix well, and obtain a mixed glue liquid.

[0045] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 24wt%, the glue is discharged, pre-cooled at 2°C, solidified, and formed into a glue block.

[0046] S6. Dry the rubber block obtained in S5 at 16° C. and 55% humidity for 72 hours, and then cut and erase the rubber to obtain a film.

[0047] Comparative Example 1

[0048] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0049] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0050] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0051] S3. Transfer the treated glue in S2 into a double-layer pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65%, add water (water temperature 25°C), boil it over high heat, then continue to concentrate it over low heat until the moisture content of the glue liquid is 65%, then add water (water temperature 25°C), boil it again, and concentrate it to obtain a purified glue liquid with a moisture content of 65wt%.

[0052] S4. Filter the glue liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of Xanthoceras sorbifolia oil, 1 part of rice wine, 1 part of sea buckthorn extract, and 1 part of ginkgo leaf extract in sequence, mix well, and obtain a mixed glue liquid.

[0053] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0054] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0055] Comparative Example 2

[0056] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0057] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0058] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0059] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65wt%, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 65wt%. Then, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 65wt%.

[0060] S4. Filter the glue liquid purified in S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of soybean oil, 1 part of rice wine, 1 part of sea buckthorn extract, and 1 part of ginkgo leaf extract in sequence, mix well, and obtain a mixed glue liquid.

[0061] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0062] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0063] Comparative Example 3

[0064] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0065] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0066] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0067] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65wt%, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 65wt%. Then, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 65wt%.

[0068] S4. Filter the glue liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it. When the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of Xanthoceras sorbifolia oil and 1 part of rice wine in turn, mix well, and obtain a mixed glue liquid.

[0069] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0070] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0071] Comparative Example 4

[0072] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0073] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0074] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0075] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65wt%, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 65wt%. Then, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 65wt%.

[0076] S4. Filter the glue liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the glue liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of Xanthoceras sorbifolia oil, 1 part of rice wine and 1 part of sea buckthorn extract in turn, mix well to obtain a mixed glue liquid.

[0077] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0078] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0079] Comparative Example 5

[0080] A method for preparing donkey-hide gelatin for protecting female ovarian and breast functions comprises the following steps:

[0081] S1, according to the quality standard of donkey skin, donkey skin is selected, cleaned and soaked, haired and fleshed, skinned and washed to obtain strip donkey skin. According to 0.5 times of the weight of donkey skin, 0.5% sodium carbonate aqueous solution is added to the donkey skin, and the steam pressure is maintained at 0.14Mpa for 40min to obtain the blanched donkey skin.

[0082] S2, placing 100 parts of the blanched donkey skin of S1 in an extraction device, adding water 0.6 times the mass of the blanched donkey skin for heating and boiling, and introducing steam to maintain the steam pressure at 0.14Mpa for extraction for 7 hours. Filtering the gum juice for slag removal; then repeating the above process of adding water, heating and boiling, and introducing steam for extraction. After combining the gum juices extracted twice, the treated gum juice is obtained.

[0083] S3. Transfer the treated glue juice in S2 into a sandwich pot, boil it over high heat, then continue to concentrate it over low heat. When the moisture content of the glue liquid is 65wt%, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it over high heat, and concentrate it over low heat until the moisture content of the glue liquid is 65wt%. Then, add water (water temperature 25°C) to the liquid level of the glue juice before concentration, boil it again, and concentrate it. Repeat the high heat boiling and low heat concentration for 9 times to obtain a purified glue liquid with a moisture content of 65wt%.

[0084] S4. Filter the gelatin liquid purified by S3 through 200 mesh and 800 mesh respectively while it is hot, transfer it into a sandwich pot, heat and concentrate it, and when the gelatin liquid is concentrated to the point where beads can be picked up with a rubber spatula, add 3 parts of crystal sugar, 3 parts of Xanthoceras sorbifolia oil, 1 part of rice wine and 1 part of Ginkgo biloba leaf extract in sequence, mix well, and obtain a mixed gelatin liquid.

[0085] S5. Concentrate the mixed glue solution of S4 to a hanging flag. When the water content is 30wt%, produce the glue, pre-cool it at 7°C, solidify it at 2°C, and shape it into a glue block.

[0086] S6. Dry the rubber block obtained in S5 at 26° C. and 70% humidity for 48 hours, and then cut and erase the rubber to obtain a film.

[0087] Efficacy Test

[0088] 1. Experiment on the effect of premature ovarian failure rat model

[0089] Fifty four-week-old female mice were randomly divided into five groups. Among them, (1) normal control group; (2) PCOS (polycystic ovary syndrome) model group, PCOS mouse animal model was established by hormone combined with high-fat feeding; (3) Example 1 donkey-hide gelatin treatment group, PCOS mice were given 0.5g / kg donkey-hide gelatin. After 21 days of continuous modeling, the above experimental groups were fed for 28 days. The levels of reproductive-related hormones (testosterone T, estrogen E2, follicle-stimulating hormone FSH, luteinizing hormone LH) were detected, and the results are shown in Table 1. The ovarian ovulation was detected, and the results are shown in Table 2.

[0090] Table 1 Reproductive hormone content

[0091]

[0092]

[0093] Table 2 Ovarian ovulation

[0094] Sample set Number of eggs Oocyte abnormality rate Blank Group 18 16.67％ Model Group 16 43.75％ Example 1 36 8.33％ Comparative Example 2 28 14.29％ Comparative Example 3 20 15.00％ Comparative Example 4 28 10.71％ Comparative Example 5 26 15.38％

[0095] 2. Experiment on the proliferation activity of mammary epithelial cells and the effect of estrogen expression level

[0096] Mouse mammary epithelial cells (purchased from Beijing Beina Chuanglian Biotechnology Research Institute) were cultured in DMEM high-glucose medium containing 10% FBS at 37°C and 5% CO2. 4×10 4 The cell suspension of 100 μg / L was inoculated in a 96-well culture plate. After stabilization for 24 hours, the drug (1% aqueous solution of donkey-hide gelatin tablets) was added, and a blank control group, an estradiol group (model group, 250 μg / mL), and an example combination group were set up respectively; after the drug acted for 48 hours, MTT dye was added, and after removing the supernatant and adding DMSO, the absorbance (A) value of each well was measured by an enzyme-linked immunosorbent assay at 490 nm, and the cell proliferation rate was calculated according to the average A value of each group.

[0097] Cell proliferation rate (%) = A value of experimental group / A value of blank group × 100%.

[0098] After the cells were treated, the cell supernatant was collected and the estrogen receptor (ER) content of the cells was measured by ELISA method. The specific operation was carried out according to the instructions of the kit. The results are shown in Table 3.

[0099] Table 3 Proliferation activity and estrogen expression level of mammary epithelial cells

[0100] Sample set Cell proliferation rate (%) ER content (ug / ml) Blank Group 101.45 125.64 Model Group 125.34 165.90 Example 1 138.90 178.10 Example 2 134.67 164.66 Comparative Example 1 108.45 130.45 Comparative Example 2 121.23 146.80 Comparative Example 3 102.34 133.67 Comparative Example 4 110.56 148.23 Comparative Example 5 109.11 150.34

[0101] Sensory evaluation

[0102] The sensory analysis method used a quantitative descriptive sensory analysis method with a score of 0 to 5 (0 points: rough surface, dull; 5 points: smooth surface, high gloss. 0 points: no light transmittance; 5 points: no cracks in the inner layer of the film, good light transmittance). The sensory analysis team consisted of 6 people, including 4 women aged 23 to 30 and 2 men aged 23 to 30. They were asked to score the donkey-hide gelatin tablets. The average of the results was taken, and the results are shown in Table 4.

[0103] Table 4 Sensory evaluation of donkey-hide gelatin film

[0104]

[0105]

[0106] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A donkey-hide gelatin for protecting female ovarian and breast functions, characterized in that: The raw materials of donkey-hide gelatin include donkey skin, crystal sugar, Xanthoceras sorbifolia oil, yellow rice wine, sea buckthorn extract and ginkgo leaf extract.

2. The donkey-hide gelatin according to claim 1, characterized in that The donkey-hide gelatin raw material includes, by weight, 80-100 parts of donkey hide, 2-3 parts of crystal sugar, 2-3 parts of Xanthoceras sorbifolia oil, 0.5-1 part of yellow rice wine, 0.1-1 part of sea buckthorn extract, and 0.1-1 part of ginkgo leaf extract.

3. The donkey-hide gelatin according to claim 2, characterized in that The mass ratio of Chinese pterocarpus oil: rock sugar: yellow wine in the donkey-hide gelatin raw material is 0.8-1:1:0.

2.

4. A method for preparing donkey-hide gelatin for protecting female ovarian and breast function according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1, selecting, cleaning, soaking, dehairing and defleshing, cutting, washing and blanching donkey skin to obtain blanched donkey skin; S2, the blanched donkey skin of S1 is placed in an extraction device, water is added, heated and steam is introduced to extract twice, the glue is filtered to remove the residue, and the glue obtained twice is combined to obtain the processed glue; S3, the glue juice treated in S2 is transferred to a sandwich pot, boiled over high heat, then concentrated over low heat until the moisture content of the glue solution is 50-65%, add 20-25°C water, boil over high heat, then concentrate over low heat until the moisture content of the glue solution is 50-65%, then add 20-25°C water, boil again, and concentrate, repeat this process 9 times to obtain a purified glue solution; S4, filtering the purified glue solution in S3 through 200 mesh and 800 mesh respectively while hot, transferring it into a sandwich pot, heating and concentrating it, and when the glue solution is concentrated to the point where hanging beads can be picked up with a rubber spatula, adding crystal sugar, Xanthoceras sorbifolia oil, rice wine, sea buckthorn extract, and ginkgo leaf extract in sequence, mixing them evenly, to obtain a mixed glue solution; S5, concentrating the mixed glue solution of S4 to a hanging flag, and when the water content is 24-30wt%, discharging the glue, precooling, solidifying, and forming into a glue block; S6, drying, cutting and rubbing the rubber block obtained in S5 to obtain a film.

5. The method for preparing donkey-hide gelatin according to claim 4, characterized in that: The blanching of the skin described in S1 is performed under steam pressure for 40 minutes at a ratio of skin weight to alkaline water weight of 1:0.5, wherein the alkaline water is a 0.5% sodium carbonate aqueous solution, and the steam pressure value is 0.12-0.15MPa.

6. The method for preparing donkey-hide gelatin according to claim 4, characterized in that: In S2, water is added according to the mass ratio of the donkey skin after blanching in S1 to water being 1:0.

6.

7. The method for preparing donkey-hide gelatin according to claim 4, characterized in that: The water content of the purified glue solution described in S3 is 50-65wt%.

8. The method for preparing donkey-hide gelatin according to claim 4, characterized in that: The precooling temperature described in S5 is 2-7°C, and the solidification temperature is 0-2°C.

9. The method for preparing donkey-hide gelatin according to claim 4, characterized in that: The drying temperature in S6 is 16-26° C., and the humidity is 55%-70%.

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