High-pressure microjet homogenizing system and process method

Abstract

The invention provides a high-pressure micro-jet homogenizing system and process method, and relates to the technical field of high-pressure homogenizing equipment, the high-pressure micro-jet homogenizing system comprises a pneumatic diaphragm pump, a first plunger pump, a second plunger pump, a diamond interaction cavity, a heat exchanger and a storage container; the pneumatic diaphragm pump, the first plunger pump, the second plunger pump, the diamond interaction containing cavity, the heat exchanger and the storage container form a material homogenizing pipeline, a CIP cleaning pipeline and an SIP sterile heating pipeline through pipelines, and a liposome extrusion pipeline is connected into the material homogenizing pipeline. According to the high-pressure microjet homogenizer, the flow of the high-pressure microjet homogenizer is increased to a great extent by using the double plunger pumps, particles and precipitates in a product are reduced by arranging the lipidosome extrusion pipeline, and the product quality is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of high-pressure homogenization equipment, and in particular to a high-pressure micro-jet homogenization system; in addition, the present invention also relates to a high-pressure micro-jet homogenization process method. Background Art

[0002] With the continuous improvement of nanotechnology, nano-homogenization technology has been widely used in various fields. For example, in the fields of food, cosmetics, chemical industry, energy, etc., high-pressure micro-jet homogenization methods are often used to improve product performance and quality. However, the existing high-pressure micro-jet homogenizer has a small flow rate, which is far from meeting the needs of customers in many fields, and lacks liposome extrusion function, which leads to excessive particles and precipitation in the product, affecting product quality. Summary of the invention

[0003] In order to solve the problems existing in the prior art, at least one embodiment of the present invention provides a high-pressure microfluidic homogenization system, which greatly increases the flow rate of the high-pressure microfluidic homogenizer and reduces particles and precipitation in the product. To this end, at least one embodiment of the present invention also provides a high-pressure microfluidic homogenization process method.

[0004] In the first aspect, an embodiment of the present invention proposes a high-pressure microfluidic homogenization system, including a pneumatic diaphragm pump, a first plunger pump, a second plunger pump, a diamond interactive cavity, a heat exchanger and a storage container; the pneumatic diaphragm pump, the first plunger pump, the second plunger pump, the diamond interactive cavity, the heat exchanger and the storage container form a material homogenization pipeline, a CIP cleaning pipeline and a SIP sterile heating pipeline through pipelines, and a liposome extrusion pipeline is connected to the material homogenization pipeline.

[0005] In some embodiments, the present invention provides a high-pressure micro-jet homogenization system, wherein the material homogenization pipeline further includes a feed port, a feed container, a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a discharge port; in the material homogenization pipeline, the feed port, the feed container, the first valve and the pneumatic diaphragm pump are connected in sequence through pipelines, the pneumatic diaphragm pump is respectively connected to the first plunger pump and the second plunger pump through pipelines, the first plunger pump and the second plunger pump are respectively connected to the diamond interaction cavity through pipelines; the diamond interaction cavity, the second valve and the heat exchanger are connected in sequence through pipelines, the heat exchanger is respectively connected to the third valve and the fifth valve through pipelines; the third valve, the material storage container and the fourth valve are connected in sequence through pipelines, the fourth valve is connected to the input port of the pneumatic diaphragm pump through a pipeline; the fifth valve is connected to the discharge port through a pipeline.

[0006] In some embodiments, in a high-pressure microfluidic homogenization system provided by the present invention, the output port of the pneumatic diaphragm pump and the input port, output port and cooling water outlet of the heat exchanger are all provided with temperature sensors.

[0007] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, wherein the liposome extrusion pipeline includes a liposome extruder, a sixth valve, a seventh valve, an eighth valve, a twenty-fourth valve, a twenty-fifth valve and a discharge port, the first plunger pump and the second plunger pump are respectively connected to the sixth valve through a pipeline, the sixth valve, the seventh valve, the liposome extruder and the eighth valve are sequentially connected through a pipeline, and the eighth valve is respectively connected to the heat exchanger and the second valve through a pipeline; the discharge port of the liposome extruder is respectively connected to the twenty-fourth valve and the twenty-fifth valve through a pipeline, and the twenty-fourth valve and the twenty-fifth valve are respectively connected to the discharge port through a pipeline.

[0008] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, wherein the CIP cleaning pipeline includes a cleaning port, a ninth valve, a CIP material homogenization cleaning pipeline, a CIP material circulation homogenization cleaning pipeline, a CIP plunger cleaning pipeline, a CIP plunger jacket cleaning pipeline, and a CIP cavity cleaning pipeline; The CIP material homogenization cleaning pipeline also includes a tenth valve and an eleventh valve. In the CIP material homogenization cleaning pipeline, the cleaning port, the ninth valve and the pneumatic diaphragm pump are connected in sequence through a pipeline, the pneumatic diaphragm pump is respectively connected to the first plunger pump and the second plunger pump through pipelines, the first plunger pump and the second plunger pump are respectively connected to the sixth valve through pipelines, the sixth valve, the tenth valve and the diamond interactive cavity jacket are connected in sequence through pipelines, and the bottom outlet of the diamond interactive cavity jacket, the eleventh valve and the discharge port are connected in sequence through pipelines; The CIP material circulation homogenization cleaning pipeline also includes a twelfth valve. In the CIP material circulation homogenization cleaning pipeline, the cleaning port, the ninth valve, the fourth valve, the material storage container, the third valve, the heat exchanger and the second valve are sequentially connected through pipelines. The second valve is connected to the diamond interaction chamber and the twelfth valve through pipelines. The twelfth valve is connected to the diamond interaction chamber jacket through pipelines. The bottom outlet of the diamond interaction chamber jacket, the eleventh valve and the discharge port are sequentially connected through pipelines. The CIP plunger cleaning pipeline also includes a thirteenth valve, a fourteenth valve and a fifteenth valve. In the CIP plunger cleaning pipeline, the cleaning port, the ninth valve, the pneumatic diaphragm pump, the thirteenth valve and the fourteenth valve are connected in sequence through pipelines. The fourteenth valve is respectively connected to the plunger of the first plunger pump and the plunger of the second plunger pump through pipelines. The plunger of the first plunger pump and the plunger of the second plunger pump are respectively connected to the fifteenth valve through pipelines. The fifteenth valve is connected to the discharge port through a pipeline. The CIP plunger jacket cleaning pipeline also includes a sixteenth valve, a seventeenth valve and an eighteenth valve. In the CIP plunger jacket cleaning pipeline, the cleaning port, the ninth valve, the pneumatic diaphragm pump, the thirteenth valve and the sixteenth valve are sequentially connected through pipelines. The sixteenth valve is respectively connected to the jacket of the first plunger pump and the jacket of the second plunger pump through pipelines. The jacket of the first plunger pump and the jacket of the second plunger pump are respectively connected to the seventeenth valve through pipelines. The seventeenth valve, the eighteenth valve and the discharge port are sequentially connected through pipelines. In the CIP cavity cleaning pipeline, the cleaning port, the ninth valve, the pneumatic diaphragm pump and the thirteenth valve are connected in sequence through pipelines, the thirteenth valve is respectively connected to the first plunger pump and the second plunger pump through pipelines, the first plunger pump and the second plunger pump are respectively connected to the sixth valve through pipelines, the sixth valve, the diamond interactive cavity and the twelfth valve are connected in sequence through pipelines, the twelfth valve is connected to the diamond interactive cavity jacket through a pipeline, and the bottom outlet of the diamond interactive cavity jacket, the eleventh valve and the discharge port are connected in sequence through pipelines.

[0009] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, the system also includes a plunger cooling pipeline, the plunger cooling pipeline includes a plunger cooling water inlet, a nineteenth valve, a twentieth valve and a plunger cooling water outlet; the plunger cooling water inlet is connected to the nineteenth valve through a pipeline, the nineteenth valve is respectively connected to the plunger of the first plunger pump and the plunger of the second plunger pump through pipelines, the plunger of the first plunger pump and the plunger of the second plunger pump are respectively connected to the twentieth valve and the fifteenth valve through pipelines, the twentieth valve is connected to the plunger cooling water outlet through a pipeline, and the fifteenth valve is connected to the discharge port through a pipeline.

[0010] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, wherein the SIP sterile heating pipeline includes a first SIP preheating pipeline, a second SIP preheating pipeline, a third SIP preheating pipeline, a first SIP cooling pipeline, a second SIP cooling pipeline, and a third SIP cooling pipeline; The first SIP preheating pipeline also includes a steam inlet, a twenty-ninth valve, a thirtieth valve and a thirty-first valve. In the first SIP preheating pipeline, the steam inlet is connected to the thirty-first valve through a pipeline, and the thirty-first valve is respectively connected to the twenty-ninth valve and the pneumatic diaphragm pump through pipelines; the output port of the pneumatic diaphragm pump is respectively connected to the first plunger pump and the second plunger pump through pipelines, and the first plunger pump and the second plunger pump are respectively connected to the sixth valve through pipelines; the sixth valve, the tenth valve and the diamond interactive chamber jacket are connected in sequence through pipelines, and the bottom outlet of the diamond interactive chamber jacket is respectively connected to the thirtieth valve and the eleventh valve through pipelines, and the twenty-ninth valve, the thirtieth valve and the eleventh valve are respectively connected to the discharge port through pipelines; In the second SIP preheating pipeline, the diamond interactive chamber jacket, the twelfth valve, and the heat exchanger are sequentially connected through pipelines, the heat exchanger is respectively connected to the fifth valve and the third valve through pipelines, and the third valve, the storage container, the fourth valve, and the pneumatic diaphragm pump are sequentially connected through pipelines; In the third SIP preheating pipeline, the thirteenth valve is respectively connected to the pneumatic diaphragm pump, the first plunger pump and the second plunger pump through pipelines, and the thirteenth valve is respectively connected to the fourteenth valve and the sixteenth valve through pipelines; the fourteenth valve is respectively connected to the plunger of the first plunger pump and the plunger of the second plunger pump through pipelines, the plunger of the first plunger pump and the plunger of the second plunger pump are respectively connected to the fifteenth valve through pipelines, and the fifteenth valve is connected to the discharge port through pipelines; the sixteenth valve is respectively connected to the jacket of the first plunger pump and the jacket of the second plunger pump through pipelines, the jacket of the first plunger pump and the jacket of the second plunger pump are respectively connected to the seventeenth valve through pipelines, the seventeenth valve is respectively connected to the eighteenth valve and the discharge port through pipelines, and the eighteenth valve is connected to the fifteenth valve through pipelines; The first SIP cooling pipeline also includes a sterile air inlet and a twenty-eighth valve. In the first SIP cooling pipeline, the sterile air inlet, the twenty-eighth valve, the pneumatic diaphragm pump and the thirteenth valve are connected in sequence through pipelines; the thirteenth valve is respectively connected to the pneumatic diaphragm pump, the first plunger pump and the second plunger pump through pipelines, and the thirteenth valve is respectively connected to the fourteenth valve and the sixteenth valve through pipelines; the fourteenth valve is respectively connected to the plunger of the first plunger pump and the plunger of the second plunger pump through pipelines, the plunger of the first plunger pump and the plunger of the second plunger pump are respectively connected to the fifteenth valve through pipelines, and the fifteenth valve is connected to the discharge port through pipelines; the sixteenth valve is respectively connected to the jacket of the first plunger pump and the jacket of the second plunger pump through pipelines, the jacket of the first plunger pump and the jacket of the second plunger pump are respectively connected to the seventeenth valve through pipelines, the seventeenth valve is respectively connected to the eighteenth valve through pipelines, and the eighteenth valve is connected to the fifteenth valve through pipelines; In the second SIP cooling pipeline, the sterile air inlet, the twenty-eighth valve, the fourth valve, the material storage container, the third valve, the heat exchanger, the second valve, the twelfth valve and the diamond interactive cavity jacket are sequentially connected through pipelines, and the bottom outlet of the diamond interactive cavity jacket, the eleventh valve and the discharge port are sequentially connected through pipelines; In the third SIP cooling pipeline, the sterile air inlet, the twenty-eighth valve and the pneumatic diaphragm pump are connected in sequence through pipelines, the output port of the pneumatic diaphragm pump is respectively connected to the first plunger pump and the second plunger pump through pipelines, the first plunger pump and the second plunger pump are respectively connected to the sixth valve through pipelines, the sixth valve, the tenth valve and the diamond interactive cavity jacket are connected in sequence through pipelines, and the bottom outlet of the diamond interactive cavity jacket, the eleventh valve and the discharge port are connected in sequence through pipelines.

[0011] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, the system also includes a heat exchange cooling pipeline, the heat exchange cooling pipeline includes a heat exchange cooling inlet, a twenty-first valve, a twenty-second valve, a heat exchange cooling outlet and a twenty-third valve; the heat exchange cooling inlet, the twenty-first valve and the cooling water inlet of the heat exchanger are connected in sequence through pipelines, the cooling water outlet of the heat exchanger is respectively connected to the twenty-second valve and the twenty-third valve through pipelines, the twenty-second valve is connected to the heat exchange cooling outlet through a pipeline, and the twenty-third valve is connected to the discharge port through a pipeline.

[0012] In some embodiments, the present invention provides a high-pressure microfluidic homogenization system, which also includes a gas inlet, a twenty-sixth valve, a pneumatic flow valve, a twenty-seventh valve and a gas exhaust port, the gas inlet is connected to the twenty-sixth valve and the twenty-seventh valve through pipelines, respectively, the twenty-seventh valve is connected to the air inlet of the pneumatic diaphragm pump through a pipeline, and the exhaust hole of the pneumatic diaphragm pump is connected to the gas exhaust port through a pipeline; the twenty-sixth valve is connected to the pneumatic flow valve through a pipeline, and the pneumatic flow valve is connected to the twenty-first valve through a pipeline.

[0013] In a second aspect, an embodiment of the present invention further provides a high-pressure microfluidic homogenization process method, including the high-pressure microfluidic homogenization system of the first aspect, including: After the exhaust of the material homogenizing pipeline is completed, the material is fed, the material is circulated and homogenized through the material homogenizing pipeline, the material is heat exchanged through the heat exchange cooling pipeline, the material is liposome extruded through the liposome extrusion pipeline, and the plunger of the plunger pump is cooled through the plunger cooling pipeline; After the material is homogenized, the high-pressure micro-jet homogenization system sequentially cleans the CIP material homogenization cleaning pipeline, CIP material circulation homogenization cleaning pipeline, CIP plunger cleaning pipeline, CIP plunger jacket cleaning pipeline, and CIP cavity cleaning pipeline. The water pressure during cleaning is greater than 3 bar, and the cleaning time is 1-10 minutes. The high-pressure microfluidic homogenization system preheats the pipeline in sections through the first SIP preheating pipeline, the second SIP preheating pipeline, and the third SIP preheating pipeline. When the three sections of the pipeline are preheated to above 100 degrees Celsius, the three sections of the pipeline are simultaneously preheated to 121 degrees Celsius while maintaining pressure; when all the pipelines are preheated to above 121 degrees Celsius, they are sterilized by heat preservation for 30 minutes; after the heat preservation and sterilization is completed, the high-pressure microfluidic homogenization system cools the pipeline through the first SIP cooling pipeline, the second SIP cooling pipeline, and the third SIP cooling pipeline, and fills with pressure-maintaining gas.

[0014] It can be seen that the high-pressure microfluidic homogenization system and process method of the embodiment of the present invention greatly increases the flow rate of the high-pressure microfluidic homogenizer by using a double-plunger pump, and reduces the particles and precipitation in the product by setting a liposome extrusion pipeline, thereby improving the product quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0016] Figure 1 Shown is a schematic structural diagram of a high-pressure microfluidic homogenization system in an embodiment of the present invention; Figure 2 Shown is a schematic diagram of a material homogenizing pipeline in an embodiment of the present invention; Figure 3 Shown is a schematic diagram of a pipeline for material circulation high-pressure microfluidic homogenization in an embodiment of the present invention; Figure 4 Shown is a schematic diagram of a pipeline for discharging materials after homogenization in an embodiment of the present invention; Figure 5 Shown is a schematic diagram of a liposome extrusion pipeline in an embodiment of the present invention; Figure 6 Shown is a schematic diagram of a pipeline for discharging materials after liposome extrusion in an embodiment of the present invention; Figure 7 Shown is a schematic diagram of a CIP material homogenization cleaning pipeline in an embodiment of the present invention; Figure 8 Shown is a schematic diagram of a CIP material circulation homogenization cleaning pipeline in an embodiment of the present invention; Fig. 9 Shown is a schematic diagram of a CIP plunger cleaning pipeline in an embodiment of the present invention; Fig.10 Shown is a schematic diagram of a CIP plunger jacket cleaning pipeline in an embodiment of the present invention; Fig.11 Shown is a schematic diagram of a CIP cavity cleaning pipeline in an embodiment of the present invention; Fig.12 Shown is a schematic diagram of a first SIP preheating pipeline in an embodiment of the present invention; Fig.13 Shown is a schematic diagram of a second SIP preheating pipeline in an embodiment of the present invention; Fig.14 Shown is a schematic diagram of a third SIP preheating pipeline in an embodiment of the present invention; Fig.15 Shown is a schematic diagram of a first SIP cooling pipeline in an embodiment of the present invention; Fig.16 Shown is a schematic diagram of a second SIP cooling pipeline in an embodiment of the present invention; Fig.17 Shown is a schematic diagram of a third SIP cooling pipeline in an embodiment of the present invention.

[0017] The reference numerals in the accompanying drawings of the specification are as follows: Pneumatic diaphragm pump 101, first plunger pump 102, second plunger pump 103, diamond interactive chamber 104, diamond interactive chamber jacket 1041, heat exchanger 105, storage container 106, liposome extruder 107, pneumatic flow valve 108, temperature sensor 109, feed container 110, feed port 201, discharge port 202, discharge port 203, cleaning port 204, plunger cooling water inlet 205, plunger cooling water outlet 206, steam inlet 207, sterile air inlet 208, heat exchange cooling inlet 209, heat exchange cooling outlet 210, gas inlet 211, gas outlet 212, first valve 301, second valve 302, third valve 303, fourth valve 304, fifth valve 305, sixth valve 306, seventh valve 307, eighth valve 308, ninth valve 309, tenth valve 310, eleventh valve 311, twelfth valve 312, thirteenth valve 313, fourteenth valve 314, fifteenth valve 315, sixteenth valve 316, seventeenth valve 317, eighteenth valve 318, nineteenth valve 319, twentieth valve 320, twenty-first valve 321, twenty-second valve 322, twenty-third valve 323, twenty-fourth valve 324, twenty-fifth valve 325, twenty-sixth valve 326, twenty-seventh valve 327, twenty-eighth valve 328, twenty-ninth valve 329, thirtieth valve 330, thirty-first valve 331. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] It should be noted that, in this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. In this article, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.

[0020] [Example 1] The inventor of this solution found that in the prior art, the flow rate of the high-pressure microfluidizer is small, which is far from meeting the needs of customers in many fields, and lacks the liposome extrusion function, which leads to excessive particles and precipitation in the product, affecting the product quality. The embodiment of the present invention provides the following solution: like Figure 1 As shown, an embodiment of the present invention provides a high-pressure microfluidic homogenization system, including a pneumatic diaphragm pump 101, a first plunger pump 102, a second plunger pump 103, a diamond interactive cavity 104, a heat exchanger 105, and a material storage container 106. The pneumatic diaphragm pump 101, the first plunger pump 102, the second plunger pump 103, the diamond interactive cavity 104, the heat exchanger 105, and the material storage container 106 form a material homogenization pipeline, a CIP cleaning pipeline, and a SIP aseptic heating pipeline through pipelines, and a liposome extrusion pipeline is connected to the material homogenization pipeline.

[0021] It should be noted that the material homogenization pipeline is used to homogenize the material, and the flow rate is increased by the coordinated operation of the first plunger pump 102 and the second plunger pump 103. The liposome extrusion pipeline reduces particles and precipitation in the product, thereby meeting the needs of customers in many fields. The CIP cleaning pipeline is used to wash and clean the entire system pipeline after the material is homogenized, and the SIP sterile heating pipeline is used to heat and sterilize the entire system pipeline.

[0022] In some embodiments, Figure 2As shown, the material homogenization pipeline also includes a feed port 201, a feed container 110, a first valve 301, a second valve 302, a third valve 303, a fourth valve 304, a fifth valve 305 and a discharge port 202. In the material homogenization pipeline, the feed port 201, the feed container 110, the first valve 301 and the pneumatic diaphragm pump 101 are sequentially connected through pipelines, the pneumatic diaphragm pump 101 is respectively connected to the first plunger pump 102 and the second plunger pump 103 through pipelines, and the first plunger pump 102 and the second plunger pump 103 are respectively connected to the diamond interaction chamber 104 through pipelines. The diamond interaction chamber 104, the second valve 302 and the heat exchanger 105 are sequentially connected through pipelines, and the heat exchanger 105 is respectively connected to the third valve 303 and the fifth valve 305 through pipelines. The third valve 303, the material storage container 106 and the fourth valve 304 are connected in sequence through pipelines, and the fourth valve 304 is connected to the input port of the pneumatic diaphragm pump 101 through a pipeline. The fifth valve 305 is connected to the discharge port 202 through a pipeline.

[0023] It should be noted that the material enters the feed container 110 from the feed port 201, and after the first valve 301 is opened, the material enters the first plunger pump 102 and the second plunger pump 103 through the pneumatic diaphragm pump 101. The second valve 302 is opened, and the first plunger pump 102 and the second plunger pump 103 send the material into the diamond interaction chamber 104 at a high speed for high-pressure micro-jet homogenization, and the temperature of the material is adjusted through the heat exchanger 105. The third valve 303 is opened, and the material enters the storage container 106.

[0024] like Figure 3 As shown, after the material enters the material storage container 106, the fourth valve 304 is opened to perform material circulation and high-pressure micro-jet homogenization.

[0025] like Figure 4 As shown, when the material is homogenized, the third valve 303 is closed, the fifth valve 305 is opened, and the homogenized material is discharged from the discharge port 202 .

[0026] In some embodiments, the system further includes a heat exchange cooling pipeline, which includes a heat exchange cooling inlet 209, a twenty-first valve 321, a twenty-second valve 322, a heat exchange cooling outlet 210, and a twenty-third valve 323. The heat exchange cooling inlet 209, the twenty-first valve 321, and the cooling water inlet of the heat exchanger 105 are sequentially connected through pipelines, the cooling water outlet of the heat exchanger 105 is respectively connected to the twenty-second valve 322 and the twenty-third valve 323 through pipelines, the twenty-second valve 322 is connected to the heat exchange cooling outlet 210 through a pipeline, and the twenty-third valve 323 is connected to the discharge port 203 through a pipeline.

[0027] It should be noted that the cooling water enters from the heat exchange cooling inlet 209, and after the twenty-first valve 321 is opened, the cooling water enters the heat exchanger 105, and after the twenty-second valve 322 is opened, the cooling water flows out of the cooling cycle through the heat exchange cooling outlet 210, and finally the twenty-third valve 323 is opened, and the coolant is discharged from the discharge port 203.

[0028] The system further includes a gas inlet 211, a twenty-sixth valve 326, a pneumatic flow valve 108, a twenty-seventh valve 327 and a gas outlet 212. The gas inlet 211 is connected to the twenty-sixth valve 326 and the twenty-seventh valve 327 through pipelines, respectively. The twenty-seventh valve 327 is connected to the air inlet of the pneumatic diaphragm pump 101 through a pipeline. The exhaust hole of the pneumatic diaphragm pump 101 is connected to the gas outlet 212 through a pipeline. The twenty-sixth valve 326 is connected to the pneumatic flow valve 108 through a pipeline, and the pneumatic flow valve 108 is connected to the twenty-first valve 321 through a pipeline.

[0029] It should be noted that the gas used for pneumatics enters from the gas inlet 211, and the twenty-sixth valve 326 is opened to supply air to the pneumatic flow valve 108. The pneumatic flow valve 108 is used to control the flow of the coolant in the above-mentioned heat exchange cooling pipeline. The twenty-seventh valve 327 is opened to supply air to the pneumatic diaphragm pump 101.

[0030] In some embodiments, the output port of the pneumatic diaphragm pump 101 and the input port, output port, and cooling water outlet of the heat exchanger 105 are all equipped with temperature sensors 109, so that their real-time temperatures can be monitored, the temperature control of the entire pipeline can be improved, and the stability of material homogeneity can be increased.

[0031] In some embodiments, Figure 5 As shown, the liposome extrusion pipeline includes a liposome extruder 107, a sixth valve 306, a seventh valve 307, an eighth valve 308, a twenty-fourth valve 324, a twenty-fifth valve 325 and a discharge port 203, the first plunger pump 102 and the second plunger pump 103 are respectively connected to the sixth valve 306 through a pipeline, the sixth valve 306, the seventh valve 307, the liposome extruder 107 and the eighth valve 308 are sequentially connected through a pipeline, and the eighth valve 308 is respectively connected to the heat exchanger 105 and the second valve 302 through a pipeline. The discharge port of the liposome extruder 107 is respectively connected to the twenty-fourth valve 324 and the twenty-fifth valve 325 through a pipeline, and the twenty-fourth valve 324 and the twenty-fifth valve 325 are respectively connected to the discharge port 203 through a pipeline.

[0032] It should be noted that when the material is circulated and homogenized by high-pressure microfluidization, the sixth valve 306 is opened, and the material is extruded through the liposome extruder 107 to reduce the particles and precipitation after the material is homogenized, and then the eighth valve 308 is opened for circulating liposome extrusion.

[0033] like Figure 6 As shown, after the liposome cyclic extrusion is completed, the third valve 303 is closed, and the material after the liposome extrusion is discharged from the discharge port 202.

[0034] In some embodiments, the system further includes a plunger cooling circuit, which includes a plunger cooling water inlet 205, a nineteenth valve 319, a twentieth valve 320, and a plunger cooling water outlet 206. The plunger cooling water inlet 205 is connected to the nineteenth valve 319 through a pipeline, the nineteenth valve 319 is respectively connected to the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 through pipelines, the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 are respectively connected to the twentieth valve 320 and the fifteenth valve 315 through pipelines, the twentieth valve 320 is connected to the plunger cooling water outlet 206 through a pipeline, and the fifteenth valve 315 is connected to the discharge port 203 through a pipeline.

[0035] It should be noted that when the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 need to be cooled, cooling water enters from the plunger cooling water inlet 205. After the cooling of the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 is completed, the twentieth valve 320 is opened to let the cooling water flow out from the plunger cooling water outlet 206. Some residual cooling water can also be discharged from the discharge port 203 by opening the fifteenth valve 315.

[0036] In some embodiments, the CIP cleaning pipeline includes a cleaning port 204, a ninth valve 309, a CIP material homogenization cleaning pipeline, a CIP material circulation homogenization cleaning pipeline, a CIP plunger cleaning pipeline, a CIP plunger jacket cleaning pipeline, and a CIP cavity cleaning pipeline. It is understandable that clean water enters through the cleaning port 204, and after the ninth valve 309 is opened, the CIP material homogenization cleaning pipeline, the CIP material circulation homogenization cleaning pipeline, the CIP plunger cleaning pipeline, the CIP plunger jacket cleaning pipeline, and the CIP cavity cleaning pipeline are cleaned.

[0037] It should be noted that after the material is homogenized, the high-pressure microjet homogenization system is cleaned in sequence through the CIP material homogenization cleaning pipeline, the CIP material circulation homogenization cleaning pipeline, the CIP plunger cleaning pipeline, the CIP plunger jacket cleaning pipeline and the CIP cavity cleaning pipeline. The water pressure during cleaning is greater than 3 bar and the cleaning time is 1-10 minutes.

[0038] In some embodiments, Figure 7As shown, the CIP material homogenization cleaning pipeline also includes a tenth valve 310 and an eleventh valve 311. In the CIP material homogenization cleaning pipeline, the cleaning port 204, the ninth valve 309, and the pneumatic diaphragm pump 101 are connected in sequence through pipelines. The pneumatic diaphragm pump 101 is connected to the first plunger pump 102 and the second plunger pump 103 through pipelines, respectively. The first plunger pump 102 and the second plunger pump 103 are connected to the sixth valve 306 through pipelines, respectively. The sixth valve 306, the tenth valve 310 and the diamond interactive chamber jacket 1041 are connected in sequence through pipelines. The bottom outlet of the diamond interactive chamber jacket 1041, the eleventh valve 311 and the discharge port 203 are connected in sequence through pipelines.

[0039] It should be noted that when cleaning the CIP material homogenization cleaning pipeline, clean water enters from the cleaning port 204, and after opening the ninth valve 309, the clean water cleans the pipelines of the pneumatic diaphragm pump 101, the first plunger pump 102 and the second plunger pump 103, and after opening the sixth valve 306, the pipeline at one end of the diamond interaction cavity jacket 1041 is cleaned, and after opening the eleventh valve, the clean water is discharged from the discharge port 203.

[0040] In some embodiments, Figure 8 As shown, the CIP material circulation homogenization cleaning pipeline also includes a twelfth valve 312. In the CIP material circulation homogenization cleaning pipeline, the cleaning port 204, the ninth valve 309, the fourth valve 304, the storage container 106, the third valve 303, the heat exchanger 105 and the second valve 302 are connected in sequence through pipelines. The second valve 302 is connected to the diamond interaction chamber 104 and the twelfth valve 312 through pipelines. The twelfth valve 312 is connected to the diamond interaction chamber jacket 1041 through a pipeline. The bottom outlet of the diamond interaction chamber jacket 1041, the eleventh valve 311 and the discharge port 203 are connected in sequence through pipelines.

[0041] It should be noted that when cleaning the CIP material circulation homogenization cleaning pipeline, clean water enters from the cleaning port 204, and the ninth valve 309, the fourth valve 304 and the third valve 303 are opened respectively to clean the material circulation homogenization pipeline and the pipeline of the heat exchanger 105. The second valve 302 and the twelfth valve 312 are opened respectively to clean the pipeline at one end of the diamond interaction chamber 104 and the pipeline at the other end of the diamond interaction chamber jacket 1041, and the clean water is discharged from the discharge port 203 after the eleventh valve is opened.

[0042] In some embodiments, Fig. 9As shown, the CIP plunger cleaning pipeline also includes a thirteenth valve 313, a fourteenth valve 314 and a fifteenth valve 315. In the CIP plunger cleaning pipeline, the cleaning port 204, the ninth valve 309, the pneumatic diaphragm pump 101, the thirteenth valve 313 and the fourteenth valve 314 are connected in sequence through pipelines. The fourteenth valve 314 is respectively connected to the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 through pipelines. The plungers of the first plunger pump 102 and the second plunger pump 103 are respectively connected to the fifteenth valve 315 through pipelines. The fifteenth valve 315 is connected to the discharge port 203 through a pipeline.

[0043] It should be noted that when cleaning the CIP plunger cleaning pipeline, clean water enters from the cleaning port 204, the ninth valve 309, the thirteenth valve 313 and the fourteenth valve 314 are opened respectively, the clean water cleans the plunger pipeline, the fifteenth valve 315 is opened, and the clean water is discharged from the discharge port 203.

[0044] In some embodiments, Fig.10 As shown, the CIP plunger jacket cleaning pipeline also includes a sixteenth valve 316, a seventeenth valve 317 and an eighteenth valve 318. In the CIP plunger jacket cleaning pipeline, the cleaning port 204, the ninth valve 309, the pneumatic diaphragm pump 101, the thirteenth valve 313 and the sixteenth valve 316 are connected in sequence through pipelines. The sixteenth valve 316 is respectively connected to the jacket of the first plunger pump 102 and the jacket of the second plunger pump 103 through pipelines. The jackets of the first plunger pump 102 and the second plunger pump 103 are respectively connected to the seventeenth valve 317 through pipelines. The seventeenth valve 317, the eighteenth valve 318 and the discharge port 203 are connected in sequence through pipelines.

[0045] It should be noted that when cleaning the CIP plunger jacket cleaning pipeline, clean water enters from the cleaning port 204, and the ninth valve 309, the thirteenth valve 313, the fourteenth valve 314 and the sixteenth valve 316 are opened respectively, and the clean water cleans the pipeline of the plunger jacket, and the eighteenth valve 318 is opened, and the clean water is discharged from the discharge port 203.

[0046] In some embodiments, Fig.11As shown, in the CIP cavity cleaning pipeline, the cleaning port 204, the ninth valve 309, the pneumatic diaphragm pump 101 and the thirteenth valve 313 are connected in sequence through pipelines, the thirteenth valve 313 is respectively connected to the first plunger pump 102 and the second plunger pump 103 through pipelines, the first plunger pump 102 and the second plunger pump 103 are respectively connected to the sixth valve 306 through pipelines, the sixth valve 306, the diamond interactive cavity 104, and the twelfth valve 312 are connected in sequence through pipelines, the twelfth valve 312 is connected to the diamond interactive cavity jacket 1041 through a pipeline, and the bottom outlet of the diamond interactive cavity jacket 1041, the eleventh valve 311 and the discharge port 203 are connected in sequence through pipelines.

[0047] It should be noted that when cleaning the CIP cavity cleaning pipeline, clean water enters from the cleaning port 204, and the ninth valve 309 and the twelfth valve 312 are opened respectively to clean the pipeline of the diamond interaction cavity, and the eleventh valve 311 is opened, and the clean water is discharged from the discharge port 203.

[0048] In some embodiments, a detergent is added to the CIP material circulation homogenization cleaning pipeline for circulation washing. Specifically, Figure 3 As shown, detergent is added to the storage container 106, and the second valve 302 and the third valve 303 are opened respectively to perform cyclic washing. Figure 4 As shown, finally, the third valve 303 is closed, the fifth valve 305 is opened, and the detergent is discharged from the discharge port 202.

[0049] In some embodiments, the SIP aseptic heating circuit includes a first SIP preheating circuit, a second SIP preheating circuit, a third SIP preheating circuit, a first SIP cooling circuit, a second SIP cooling circuit, and a third SIP cooling circuit.

[0050] It should be noted that the high-pressure microfluidic homogenization system performs segmented preheating of the pipelines through the first SIP preheating pipeline, the second SIP preheating pipeline, and the third SIP preheating pipeline. When the three pipelines are preheated to above 100 degrees Celsius, the three pipelines are simultaneously preheated to 121 degrees Celsius while maintaining pressure. Specifically, when the lowest temperature points of the three pipelines are respectively preheated to above 100 degrees Celsius, the three pipelines are simultaneously preheated to 121 degrees Celsius while maintaining pressure.

[0051] When all pipelines are preheated to above 121 degrees Celsius, they are sterilized by heat preservation for 30 minutes. After the heat preservation and sterilization is completed, the high-pressure microfluidic homogenization system cools the pipelines through the first SIP cooling pipeline, the second SIP cooling pipeline, and the third SIP cooling pipeline, and is filled with pressure-maintaining gas, specifically, the pressure-maintaining gas can be nitrogen.

[0052] In some embodiments, Fig.12As shown, the first SIP preheating pipeline also includes a steam inlet 207, a twenty-ninth valve 329, a thirtieth valve 330 and a thirty-first valve 331. In the first SIP preheating pipeline, the steam inlet 207 is connected to the thirty-first valve 331 through a pipeline, and the thirty-first valve 331 is respectively connected to the twenty-ninth valve 329 and the pneumatic diaphragm pump 101 through pipelines. The output port of the pneumatic diaphragm pump 101 is respectively connected to the first plunger pump 102 and the second plunger pump 103 through pipelines, and the first plunger pump 102 and the second plunger pump 103 are respectively connected to the sixth valve 306 through pipelines. The sixth valve 306, the tenth valve 310, and the diamond interactive chamber jacket 1041 are connected in sequence through pipelines, and the bottom outlet of the diamond interactive chamber jacket 1041 is respectively connected to the thirtieth valve 330 and the eleventh valve 311 through pipelines, and the twenty-ninth valve 329, the thirtieth valve 330 and the eleventh valve 311 are respectively connected to the discharge port 203 through pipelines.

[0053] It should be noted that hot steam enters from the steam inlet 207, and the 31st valve 331, the 29th valve 329 and the 10th valve 310 are opened respectively, so as to preheat the pipeline of the pneumatic diaphragm pump 101, the pipeline of the first plunger pump 102 and the second plunger pump 103, and the front pipeline of the diamond interaction chamber 104. At the minimum pressure, the first plunger pump 102 and the second plunger pump 103 operate automatically periodically. The 30th valve 330 and the 11th valve 311 are opened respectively, and the hot steam is discharged from the discharge port 203.

[0054] In some embodiments, Fig.13 As shown, a second SIP preheating pipeline is added on the basis of the first SIP preheating pipeline, and the second SIP preheating pipeline is preheated while the first SIP preheating pipeline is preheated. In the second SIP preheating pipeline, the diamond interaction chamber jacket 1041, the twelfth valve 312 and the heat exchanger 105 are connected in sequence through pipelines, the heat exchanger 105 is connected to the fifth valve 305 and the third valve 303 through pipelines, and the third valve 303, the storage container 106, the fourth valve 304 and the pneumatic diaphragm pump 101 are connected in sequence through pipelines.

[0055] It should be noted that the twelfth valve 312 , the second valve 302 , the third valve 303 and the fourth valve 304 are opened respectively, and at the minimum pressure, the first plunger pump 102 and the second plunger pump 103 automatically operate periodically to preheat the rear section management of the diamond interaction chamber 104 .

[0056] In some embodiments, Fig.14As shown, a third SIP preheating pipeline is added on the basis of the first SIP preheating pipeline and the second SIP preheating pipeline, and the third SIP preheating pipeline is preheated while the first SIP preheating pipeline and the second SIP preheating pipeline are preheated. In the third SIP preheating pipeline, the thirteenth valve 313 is respectively connected to the pneumatic diaphragm pump 101, the first plunger pump 102, and the second plunger pump 103 through pipelines, and the thirteenth valve 313 is respectively connected to the fourteenth valve 314 and the sixteenth valve 316 through pipelines. The fourteenth valve 314 is connected to the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 through pipelines, respectively. The plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 are connected to the fifteenth valve 315 through pipelines, respectively. The fifteenth valve 315 is connected to the discharge port 203 through pipelines; the sixteenth valve 316 is connected to the jacket of the first plunger pump 102 and the jacket of the second plunger pump 103 through pipelines, respectively. The jacket of the first plunger pump 102 and the jacket of the second plunger pump 103 are connected to the seventeenth valve 317 through pipelines, respectively. The seventeenth valve 317 is connected to the eighteenth valve 318 and the discharge port 203 through pipelines, respectively. The eighteenth valve 318 is connected to the fifteenth valve 315 through pipelines.

[0057] It should be noted that, by opening the thirteenth valve 313, the fourteenth valve 314, the fifteenth valve 315, the sixteenth valve 316, the seventeenth valve 317 and the eighteenth valve 318 respectively, at the minimum pressure, the first plunger pump 102 and the second plunger pump 103 automatically operate periodically to preheat the pipelines of the first plunger pump 102 and the second plunger pump 103.

[0058] In some embodiments, Fig.15As shown, the first SIP cooling line also includes a sterile air inlet 208 and a twenty-eighth valve 328. In the first SIP cooling line, the sterile air inlet 208, the twenty-eighth valve 328, the pneumatic diaphragm pump 101 and the thirteenth valve 313 are connected in sequence through pipelines. The thirteenth valve 313 is respectively connected to the pneumatic diaphragm pump 101, the first plunger pump 102 and the second plunger pump 103 through pipelines, and the thirteenth valve 313 is respectively connected to the fourteenth valve 314 and the sixteenth valve 316 through pipelines. The fourteenth valve 314 is respectively connected to the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 through pipelines, and the plunger of the first plunger pump 102 and the plunger of the second plunger pump 103 are respectively connected to the fifteenth valve 315 through pipelines, and the fifteenth valve 315 is connected to the discharge port 203 through a pipeline. The sixteenth valve 316 is connected to the jacket of the first plunger pump 102 and the jacket of the second plunger pump 103 through pipelines, the jacket of the first plunger pump 102 and the jacket of the second plunger pump 103 are connected to the seventeenth valve 317 through pipelines, the seventeenth valve 317 is connected to the eighteenth valve 318 through pipelines, and the eighteenth valve 318 is connected to the fifteenth valve 315 through a pipeline.

[0059] It should be noted that sterile air is blown into the sterile air inlet 208, and the twenty-eighth valve 328, the thirteenth valve 313, the fourteenth valve 314, the sixteenth valve 316 and the seventeenth valve 317 are opened respectively, so as to cool the first SIP cooling pipeline. After the fifteenth valve 315 and the eighteenth valve 318 are opened respectively, the sterile air is discharged from the discharge port 203, the cooling is completed, the corresponding valves are closed, and the cooling of the second SIP cooling pipeline is started.

[0060] In some embodiments, Fig.16 As shown, in the second SIP cooling circuit, the sterile air inlet 208, the twenty-eighth valve 328, the fourth valve 304, the storage container 106, the third valve 303, the heat exchanger 105, the second valve 302, the twelfth valve 312 and the diamond interactive cavity jacket 1041 are connected in sequence through pipelines, and the bottom outlet of the diamond interactive cavity jacket 1041, the eleventh valve 311 and the discharge port 203 are connected in sequence through pipelines.

[0061] It should be noted that sterile air is blown into the sterile air inlet 208, and the twenty-eighth valve 328, the fourth valve 304, the third valve 303, the second valve 302 and the twelfth valve 312 are opened respectively, so as to cool the second SIP cooling line. After the eleventh valve 311 is opened, the sterile air is discharged from the discharge port 203, the cooling is completed, the corresponding valve is closed, and the cooling of the third SIP cooling line is started.

[0062] In some embodiments, Fig.17As shown, in the third SIP cooling circuit, the sterile air inlet 208, the twenty-eighth valve 328 and the pneumatic diaphragm pump 101 are connected in sequence through pipelines, the output port of the pneumatic diaphragm pump 101 is respectively connected to the first plunger pump 102 and the second plunger pump 103 through pipelines, the first plunger pump 102 and the second plunger pump 103 are respectively connected to the sixth valve 306 through pipelines, the sixth valve 306, the tenth valve 310 and the diamond interactive cavity jacket 1041 are connected in sequence through pipelines, and the bottom outlet of the diamond interactive cavity jacket 1041, the eleventh valve 311 and the discharge port 203 are connected in sequence through pipelines.

[0063] It should be noted that sterile air is blown into the sterile air inlet 208, and the twenty-eighth valve 328 and the sixth valve 306 are opened respectively, so as to cool the third SIP cooling pipeline. After the eleventh valve 311 is opened, the sterile air is discharged from the discharge port 203. When the cooling is completed, the corresponding valve is closed, and there is gas of corresponding pressure in the pipeline to maintain the sterile environment of the pipeline.

[0064] [Example 2] The embodiment of the present invention further provides a high-pressure microfluidic homogenization process method, including the high-pressure microfluidic homogenization system of embodiment 1, the method comprising: Step 401, when the exhaust of the material homogenizing pipeline is completed, the material is fed, the material is circulated and homogenized through the material homogenizing pipeline, the material is heat exchanged through the heat exchange cooling pipeline, the material is liposome extruded through the liposome extrusion pipeline, and the plunger of the plunger pump is cooled through the plunger cooling pipeline.

[0065] It should be noted that when the material is homogenized, the flow rate is increased by the coordinated operation of the first plunger pump 102 and the second plunger pump 103, and the liposome extrusion pipeline reduces particles and precipitation in the product, thereby meeting customer needs in many fields.

[0066] In some embodiments, the output port of the pneumatic diaphragm pump 101 and the input port, output port, and cooling water outlet of the heat exchanger 105 are all equipped with temperature sensors 109, so that their real-time temperatures can be monitored, the temperature control of the entire pipeline can be improved, and the stability of material homogeneity can be increased.

[0067] Step 402, after the material is homogenized, the high-pressure micro-jet homogenization system sequentially cleans through the CIP material homogenization cleaning pipeline, the CIP material circulation homogenization cleaning pipeline, the CIP plunger cleaning pipeline, the CIP plunger jacket cleaning pipeline, and the CIP cavity cleaning pipeline. The water pressure during cleaning is greater than 3 bar, and the cleaning time is 1-10 minutes.

[0068] It should be noted that the overall cleaning times can be set according to the user. For example, the user can choose to clean once or more times, and each cleaning is performed in accordance with the above pipeline sequence.

[0069] In some embodiments, detergent is added to the CIP material circulating homogenization cleaning pipeline for circulating washing, thereby further cleaning the entire pipeline. Specifically, Figure 3 As shown, detergent is added to the storage container 106, and the second valve 302 and the third valve 303 are opened respectively to perform cyclic washing. Figure 4 As shown, finally, the third valve 303 is closed, the fifth valve 305 is opened, and the detergent is discharged from the discharge port 202.

[0070] Step 403, the high-pressure microfluidic homogenization system performs pipeline segment preheating through the first SIP preheating pipeline, the second SIP preheating pipeline, and the third SIP preheating pipeline. When the three pipelines are preheated to above 100 degrees Celsius, the three pipelines are simultaneously preheated to 121 degrees Celsius while maintaining pressure. When all pipelines are preheated to above 121 degrees Celsius, they are sterilized by heat preservation for 30 minutes. After the heat preservation and sterilization is completed, the high-pressure microfluidic homogenization system performs pipeline cooling through the first SIP cooling pipeline, the second SIP cooling pipeline, and the third SIP cooling pipeline, and is filled with pressure-maintaining gas.

[0071] It should be noted that in the first SIP preheating pipeline, the pipeline of the pneumatic diaphragm pump 101, the pipeline of the first plunger pump 102 and the second plunger pump 103, and the front pipeline of the diamond interaction chamber 104 are preheated, and the preheating time can be set as needed so that the coldest point in the pipeline is heated to above 100 degrees Celsius. In the second SIP preheating pipeline, the rear section of the diamond interaction chamber 104 is preheated, and the preheating time can be set as needed so that the coldest point in the pipeline is heated to above 100 degrees Celsius. In the third SIP preheating pipeline, the pipeline of the first plunger pump 102 and the second plunger pump 103 are preheated, and the preheating time can be set as needed so that the coldest point in the pipeline is heated to above 100 degrees Celsius. When the coldest point of all pipelines reaches above 121 degrees Celsius, 30 minutes of heat preservation and sterilization are performed. When preheating is completed, the steam is discharged and sterile air is blown in for cooling, and finally nitrogen is filled in to maintain pressure in preparation for the next production.

[0072] In summary, Examples 1-2 of the present invention provide a high-pressure microfluidic homogenization system and process method, which greatly increases the flow rate of the high-pressure microfluidic homogenizer by using a double-plunger pump, and reduces the particles and precipitation in the product by setting a liposome extrusion pipeline, thereby improving the product quality.

[0073] The above contents are only specific implementation methods of the present application, and the protection scope of the present application is not limited thereto. Those skilled in the art may make changes or substitutions within the technical scope disclosed in the present application, and these changes or substitutions shall be within the protection scope of the present application.

[0074] Those skilled in the art will appreciate that although some embodiments described herein include certain features included in other embodiments but not other features, the combination of features from different embodiments is meant to be within the scope of the present invention and to form different embodiments.

[0075] Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A high-pressure microfluidic homogenization system, characterized in that: The invention comprises an air-operated diaphragm pump (101), a first plunger pump (102), a second plunger pump (103), a diamond interaction cavity (104), a heat exchanger (105) and a material storage container (106); the air-operated diaphragm pump (101), the first plunger pump (102), the second plunger pump (103), the diamond interaction cavity (104), the heat exchanger (105) and the material storage container (106) are connected to form a material homogenization pipeline, a CIP cleaning pipeline and a SIP aseptic heating pipeline through pipelines; and a liposome extrusion pipeline is connected to the material homogenization pipeline.

2. The high-pressure microfluidic homogenization system according to claim 1, characterized in that: The material homogenizing pipeline further comprises a feed port (201), a feed container (110), a first valve (301), a second valve (302), a third valve (303), a fourth valve (304), a fifth valve (305) and a discharge port (202); in the material homogenizing pipeline, the feed port (201), the feed container (110), the first valve (301) and the pneumatic diaphragm pump (101) are sequentially connected via pipelines, the pneumatic diaphragm pump (101) is respectively connected to the first plunger pump (102) and the second plunger pump (103) via pipelines, the first plunger pump (102) and the second plunger pump (103) are respectively connected to the first plunger pump (102) and the second plunger pump (103) The diamond interaction chamber (104), the second valve (302) and the heat exchanger (105) are connected in sequence through pipelines, and the heat exchanger (105) is connected to the third valve (303) and the fifth valve (305) through pipelines; the third valve (303), the material storage container (106) and the fourth valve (304) are connected in sequence through pipelines, and the fourth valve (304) is connected to the input port of the pneumatic diaphragm pump (101) through a pipeline; and the fifth valve (305) is connected to the discharge port (202) through a pipeline.

3. The high-pressure microfluidic homogenization system according to claim 2, characterized in that: The output port of the pneumatic diaphragm pump (101) and the input port, output port and cooling water outlet of the heat exchanger (105) are all provided with temperature sensors (109).

4. The high-pressure microfluidic homogenization system according to claim 2, characterized in that: The liposome extrusion pipeline comprises a liposome extruder (107), a sixth valve (306), a seventh valve (307), an eighth valve (308), a twenty-fourth valve (324), a twenty-fifth valve (325) and a discharge port (203); the first plunger pump (102) and the second plunger pump (103) are respectively connected to the sixth valve (306) through a pipeline; the sixth valve (306), the seventh valve (307), the liposome extruder (107) and the eighth valve (308) are sequentially connected through pipelines; the eighth valve (308) is respectively connected to the heat exchanger (105) and the second valve (302) through pipelines; the discharge port of the liposome extruder (107) is respectively connected to the twenty-fourth valve (324) and the twenty-fifth valve (325) through pipelines; the twenty-fourth valve (324) and the twenty-fifth valve (325) are respectively connected to the discharge port (203) through pipelines.

5. The high-pressure microfluidic homogenization system according to claim 4, characterized in that: The CIP cleaning pipeline comprises a cleaning port (204), a ninth valve (309), a CIP material homogenization cleaning pipeline, a CIP material circulation homogenization cleaning pipeline, a CIP plunger cleaning pipeline, a CIP plunger jacket cleaning pipeline and a CIP cavity cleaning pipeline; The CIP material homogenization cleaning pipeline further comprises a tenth valve (310) and an eleventh valve (311). In the CIP material homogenization cleaning pipeline, the cleaning port (204), the ninth valve (309) and the pneumatic diaphragm pump (101) are sequentially connected via pipelines. The pneumatic diaphragm pump (101) is respectively connected to the first plunger pump (102) and the second plunger pump (103) via pipelines. The first plunger pump (102) and the second plunger pump (103) are respectively connected to the sixth valve (306) via pipelines. The sixth valve (306), the tenth valve (310) and the diamond interaction chamber jacket (1041) are sequentially connected via pipelines. The bottom outlet of the diamond interaction chamber jacket (1041), the eleventh valve (311) and the discharge port (203) are sequentially connected via pipelines. The CIP material circulation homogenization cleaning pipeline further comprises a twelfth valve (312). In the CIP material circulation homogenization cleaning pipeline, the cleaning port (204), the ninth valve (309), the fourth valve (304), the material storage container (106), the third valve (303), the heat exchanger (105) and the second valve (302) are sequentially connected via pipelines. The second valve (302) is connected to the diamond interaction chamber (104) and the twelfth valve (312) via pipelines. The twelfth valve (312) is connected to the diamond interaction chamber jacket (1041) via pipelines. The CIP plunger cleaning pipeline further comprises a thirteenth valve (313), a fourteenth valve (314) and a fifteenth valve (315). In the CIP plunger cleaning pipeline, the cleaning port (204), the ninth valve (309), the pneumatic diaphragm pump (101), the thirteenth valve (313) and the fourteenth valve (314) are connected in sequence through pipelines. The fourteenth valve (314) is respectively connected to the plunger of the first plunger pump (102) and the plunger of the second plunger pump (103) through pipelines. The plunger of the first plunger pump (102) and the plunger of the second plunger pump (103) are respectively connected to the fifteenth valve (315) through pipelines. The fifteenth valve (315) is connected to the discharge port (203) through a pipeline. The CIP plunger jacket cleaning pipeline further comprises a sixteenth valve (316), a seventeenth valve (317) and an eighteenth valve (318). In the CIP plunger jacket cleaning pipeline, the cleaning port (204), the ninth valve (309), the pneumatic diaphragm pump (101), the thirteenth valve (313) and the sixteenth valve (316) are sequentially connected via pipelines. The sixteenth valve (316) is respectively connected to the jacket of the first plunger pump (102) and the jacket of the second plunger pump (103) via pipelines. The jacket of the first plunger pump (102) and the jacket of the second plunger pump (103) are respectively connected to the seventeenth valve (317) via pipelines. The seventeenth valve (317), the eighteenth valve (318) and the discharge port (203) are sequentially connected via pipelines. In the CIP cavity cleaning pipeline, the cleaning port (204), the ninth valve (309), the pneumatic diaphragm pump (101) and the thirteenth valve (313) are connected in sequence through pipelines, the thirteenth valve (313) is connected to the first plunger pump (102) and the second plunger pump (103) through pipelines, the first plunger pump (102) and the second plunger pump (103) are connected to the sixth valve (306) through pipelines, and the sixth valve (306), the diamond interaction cavity (104) and the twelfth valve (312) are connected in sequence through pipelines.

6. The high-pressure microfluidic homogenization system according to claim 5, characterized in that: The system further comprises a plunger cooling pipeline, the plunger cooling pipeline comprising a plunger cooling water inlet (205), a nineteenth valve (319), a twentieth valve (320) and a plunger cooling water outlet (206); the plunger cooling water inlet (205) is connected to the nineteenth valve (319) via a pipeline, the nineteenth valve (319) is respectively connected to the plunger of the first plunger pump (102) and the plunger of the second plunger pump (103) via pipelines, the plunger of the first plunger pump (102) and the plunger of the second plunger pump (103) are respectively connected to the twentieth valve (320) and the fifteenth valve (315) via pipelines, the twentieth valve (320) is connected to the plunger cooling water outlet (206) via a pipeline, and the fifteenth valve (315) is connected to the discharge port (203) via a pipeline.

7. The high-pressure microfluidic homogenization system according to claim 5, characterized in that: The SIP aseptic heating pipeline includes a first SIP preheating pipeline, a second SIP preheating pipeline, a third SIP preheating pipeline, a first SIP cooling pipeline, a second SIP cooling pipeline and a third SIP cooling pipeline; The first SIP preheating pipeline further comprises a steam inlet (207), a twenty-ninth valve (329), a thirtieth valve (330) and a thirty-first valve (331). In the first SIP preheating pipeline, the steam inlet (207) is connected to the thirty-first valve (331) via a pipeline, and the thirty-first valve (331) is respectively connected to the twenty-ninth valve (329) and the pneumatic diaphragm pump (101) via pipelines; the output port of the pneumatic diaphragm pump (101) is respectively connected to the first plunger pump (102) and the second plunger pump (103) via pipelines. , the first plunger pump (102) and the second plunger pump (103) are respectively connected to the sixth valve (306) through pipelines; the sixth valve (306), the tenth valve (310) and the diamond interactive chamber jacket (1041) are sequentially connected through pipelines, the bottom outlet of the diamond interactive chamber jacket (1041) is respectively connected to the thirtieth valve (330) and the eleventh valve (311) through pipelines, and the twenty-ninth valve (329), the thirtieth valve (330) and the eleventh valve (311) are respectively connected to the discharge port (203) through pipelines; In the second SIP preheating pipeline, the diamond interaction chamber jacket (1041), the twelfth valve (312) and the heat exchanger (105) are sequentially connected via pipelines, the heat exchanger (105) is respectively connected to the fifth valve (305) and the third valve (303) via pipelines, and the third valve (303), the material storage container (106), the fourth valve (304) and the pneumatic diaphragm pump (101) are sequentially connected via pipelines; In the third SIP preheating pipeline, the thirteenth valve (313) is connected to the pneumatic diaphragm pump (101), the first plunger pump (102) and the second plunger pump (103) through pipelines, and the thirteenth valve (313) is connected to the fourteenth valve (314) and the sixteenth valve (316) through pipelines; the fourteenth valve (314) is connected to the plunger of the first plunger pump (102) and the plunger of the second plunger pump (103) through pipelines, and the plungers of the first plunger pump (102) and the second plunger pump (103) are connected to the fifteenth valve (314) through pipelines. 15), the fifteenth valve (315) is connected to the discharge port (203) through a pipeline; the sixteenth valve (316) is connected to the jacket of the first plunger pump (102) and the jacket of the second plunger pump (103) through pipelines, respectively; the jacket of the first plunger pump (102) and the jacket of the second plunger pump (103) are connected to the seventeenth valve (317) through pipelines, respectively; the seventeenth valve (317) is connected to the eighteenth valve (318) and the discharge port (203) through pipelines, respectively; the eighteenth valve (318) is connected to the fifteenth valve (315) through a pipeline; The first SIP cooling pipeline further comprises a sterile air inlet (208) and a twenty-eighth valve (328), and in the first SIP cooling pipeline, the sterile air inlet (208), the twenty-eighth valve (328), the pneumatic diaphragm pump (101) and the thirteenth valve (313) are sequentially connected via pipelines; In the second SIP cooling pipeline, the sterile air inlet (208), the twenty-eighth valve (328), the fourth valve (304), the material storage container (106), the third valve (303), the heat exchanger (105), the second valve (302), the twelfth valve (312) and the diamond interaction chamber jacket (1041) are sequentially connected through pipelines; In the third SIP cooling pipeline, the sterile air inlet (208), the twenty-eighth valve (328) and the pneumatic diaphragm pump (101) are connected in sequence through pipelines.

8. The high-pressure microfluidic homogenization system according to claim 4, characterized in that: The system further comprises a heat exchange cooling pipeline, the heat exchange cooling pipeline comprising a heat exchange cooling inlet (209), a twenty-first valve (321), a twenty-second valve (322), a heat exchange cooling outlet (210), and a twenty-third valve (323); the heat exchange cooling inlet (209), the twenty-first valve (321), and a cooling water inlet of the heat exchanger (105) are connected in sequence through pipelines, the cooling water outlet of the heat exchanger (105) is respectively connected to the twenty-second valve (322) and the twenty-third valve (323) through pipelines, the twenty-second valve (322) is connected to the heat exchange cooling outlet (210) through a pipeline, and the twenty-third valve (323) is connected to the discharge port (203) through a pipeline.

9. The high-pressure microfluidic homogenization system according to claim 8, characterized in that: The system further comprises a gas inlet (211), a twenty-sixth valve (326), a pneumatic flow valve (108), a twenty-seventh valve (327) and a gas outlet (212); the gas inlet (211) is connected to the twenty-sixth valve (326) and the twenty-seventh valve (327) via pipelines, respectively; the twenty-seventh valve (327) is connected to the gas inlet of the pneumatic diaphragm pump (101) via a pipeline; the exhaust hole of the pneumatic diaphragm pump (101) is connected to the gas outlet (212) via a pipeline; the twenty-sixth valve (326) is connected to the pneumatic flow valve (108) via a pipeline; and the pneumatic flow valve (108) is connected to the twenty-first valve (321) via a pipeline.

10. A high-pressure microfluidization homogenization process, characterized in that: The method comprises the high-pressure microfluidic homogenization system according to any one of claims 1 to 9, wherein the method comprises: After the exhaust of the material homogenizing pipeline is completed, the material is fed, the material is circulated and homogenized through the material homogenizing pipeline, the material is heat exchanged through the heat exchange cooling pipeline, the material is liposome extruded through the liposome extrusion pipeline, and the plunger of the plunger pump is cooled through the plunger cooling pipeline; After the material is homogenized, the high-pressure micro-jet homogenization system sequentially cleans through the CIP material homogenization cleaning pipeline, the CIP material circulation homogenization cleaning pipeline, the CIP plunger cleaning pipeline, the CIP plunger jacket cleaning pipeline, and the CIP cavity cleaning pipeline. The water pressure during cleaning is greater than 3 bar, and the cleaning time is 1-10 minutes. The high-pressure microfluidic homogenization system performs pipeline segment preheating through the first SIP preheating pipeline, the second SIP preheating pipeline, and the third SIP preheating pipeline. When the three pipeline segments are preheated to above 100 degrees Celsius, the three pipeline segments are simultaneously preheated to 121 degrees Celsius while maintaining pressure; when all pipelines are preheated to above 121 degrees Celsius, they are sterilized by heat preservation for 30 minutes; after the heat preservation and sterilization is completed, the high-pressure microfluidic homogenization system performs pipeline cooling through the first SIP cooling pipeline, the second SIP cooling pipeline, and the third SIP cooling pipeline, and is filled with pressure-maintaining gas.

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