Culture medium stirring control system and method for cell suspension culture

Abstract

The invention provides a culture medium stirring control system and method for cell suspension culture, and the system comprises two groups of operating tables which are used for culture medium suspension culture placement and culture medium culture operation; the cell storage bin is located on the inner side of the middle of the operation table, the cell storage bin slides on the inner side of the operation table in a lifting mode, and the cell storage bin is used for placing a plurality of culture media in a layered and airtight mode; the stirring mechanism is connected to the center of the top surface of the cell storage bin, the output end of the stirring mechanism is arranged on the inner side of the cell storage bin in a penetrating mode, and the output end of the stirring mechanism can conduct reciprocating pushing and rotating pushing on culture media placed in the cell storage bin in a layered mode; the cell culture medium can be automatically taken out and conveyed to the outer side, the opening and closing times of the cell culture medium are reduced, and the safety of the culture environment in the cell storage bin is improved.

Description

Technical Field

[0001] The present invention relates to the field of cell suspension culture, and in particular to a culture medium stirring control system and method for cell suspension culture. Background Art

[0002] Cell suspension culture is a cell culture technique performed in liquid culture medium. It is suitable for cell types that do not rely on solid surfaces for growth, such as certain types of animal cells and microorganisms. This culture method allows cells to grow freely in three-dimensional space, facilitating large-scale culture and high-throughput screening. Cell suspension culture refers to allowing cells to grow in a suspended space, and cells are always active during the growth process.

[0003] The culture space for cell suspension culture is a closed sterile space. At the same time, the sterile space is always active. The function of cell suspension culture is achieved by placing the cell culture medium in the space for growth. However, when taking and placing the culture medium in this space, the space where the culture medium is stored needs to be opened frequently. In this process, bacteria will inevitably enter the culture space.

[0004] Therefore, we have made improvements to this problem and proposed a culture medium agitation control system and method for cell suspension culture. Summary of the invention

[0005] The purpose of the present invention is to address the existing culture medium storage which requires frequent opening of the culture space of the suspension culture bin.

[0006] In order to achieve the above-mentioned purpose of the invention, the present invention provides the following culture medium agitation control system and method for cell suspension culture to improve the above-mentioned problems.

[0007] The specific application is as follows:

[0008] A culture medium agitation control system for cell suspension culture, the system comprising:

[0009] An operating table, wherein the operating table is provided with two groups, and the operating table is used for placing culture medium suspension culture and culture medium culture operation;

[0010] A cell storage bin, which is located inside the middle of a group of operating tables. The cell storage bin is lifted and slid inside the operating tables. The cell storage bin is used to place multiple culture media in layers and in a sealed manner.

[0011] A stirring mechanism, wherein the stirring mechanism is connected to the center of the top surface of the cell storage bin, and the output end of the stirring mechanism is arranged inside the cell storage bin, and the output end of the stirring mechanism can drive the culture medium placed in layers inside the cell storage bin to reciprocate and rotate;

[0012] A driving unit, the driving unit is located below the cell storage bin, and an output end of the driving unit drives the cell storage bin to rise and fall vertically;

[0013] A transfer platform, the transfer platform is located on the upper back side of the operating platform, the top surface of the transfer platform is parallel to the top surface of the operating platform, one end of the transfer platform is connected to the driving unit for transmission, and the transfer platform is used to transport the culture medium sent out from the cell storage bin;

[0014] A collecting baffle, the collecting baffle is rotatably connected to the top of another set of operating tables near the transmission table, and the collecting baffle can block the culture medium in the transportation process in the rotating state, and change the path of the culture medium to be transported to the top surface of the operating table;

[0015] A positioning component, wherein the positioning component is located at one side of the cell storage bin, the positioning component is plugged into the inner side of the outer wall of the cell storage bin for positioning, the end of the positioning component away from the cell storage bin is transmission-connected to the collection baffle, and the positioning component is synchronously driven and plugged into the cell storage bin to position the lifting position of the cell storage bin under the driving state of the collection baffle, and is used to disconnect the transmission relationship between the drive unit and the cell storage bin, and maintain the continuous output of the drive unit to the transmission platform when the lifting layer number of the cell storage bin is positioned.

[0016] As a preferred technical solution of the present application, the system also includes an intelligent incubation cabin, a sample workroom and an operation workroom are provided on the inner side of the intelligent incubation cabin, the sample workroom and the operation workroom are provided adjacent to each other, a first changing room and a second changing room are provided on the inner side of the intelligent incubation cabin, the first changing room and the second changing room are provided adjacent to each other, an instrument room is provided on the opposite side of the first changing room and the second changing room, the two groups of operating tables are respectively located on the inner side of the sample workroom and the operation workroom, and the operating tables located on the inner sides of the sample workroom and the operation workroom are placed in parallel.

[0017] As a preferred technical solution of the present application, the operating table includes a sample operating table and a work operating table, the sample operating table is located on the inner side of the sample workroom, the work operating table is located on the inner side of the operation workroom, the cell storage bin is located on the inner side of the middle part of the sample operating table, the collection baffle is located on the side of the top surface of the work operating table close to the operation workroom, the transmission table is located on the inner side of the intelligent culture chamber and is parallel to the top surfaces of the sample operating table and the work operating table, a work table is provided below the front of the sample operating table and the work operating table, a bin body closing cover is provided on the top surface of the sample operating table located above the cell storage bin, a bin body closing cover is connected to the back of the bin body closing cover, and a conveying outlet is opened between the bottom of the bin chamber closing baffle and the top surface of the sample operating table.

[0018] As a preferred technical solution of the present application, a sample chamber is provided inside the cell storage bin, and the sample chamber has multiple layers equidistantly provided from top to bottom. A sample inlet is provided on one side of the front of the cell storage bin, and the sample inlet is connected to the inside of the sample chamber. A sample outlet is provided on the back of the cell storage bin, and the sample outlet is connected to the delivery outlet. Both ends of the cell storage bin are connected to a lifting transmission frame, and a threaded drive screw is passed through the lifting transmission frame, and a transmission gear is connected to the bottom end of the drive screw.

[0019] As a preferred technical solution of the present application, the stirring mechanism includes a stirring shaft, which is vertically arranged at the center of the cell storage bin, and the outer wall of the stirring shaft is located inside the sample chamber and is fitted with a limiting combination cylinder, and symmetrical sample transfer plates are connected to both sides of the outer wall of the limiting combination cylinder, and a limiting combination ring is provided at the position where the limiting combination cylinder is connected inside the sample chamber, and the upper and lower surfaces of the limiting combination cylinder are fitted on the outside of the limiting combination ring, and a stirring motor is provided on the top surface of the cell storage bin, and the output end of the stirring motor is drivingly connected to the stirring shaft.

[0020] The transmission gear is connected with the gear train of the transmission gear through the transmission gear, and the transmission gear is meshed with the gear train of the transmission gear and the gear train is connected with the gear train of the transmission gear through the transmission gear.

[0021] As a preferred technical solution of the present application, the transmission platform includes two groups of transmission shafts, the transmission shafts are rotatably connected to the two sides of the inside of the intelligent culture cabin, the outer side of the end of the transmission shaft close to the sample operating table and the work operating table is sleeved with a conveyor roller, the outer side of the conveyor roller is sleeved with a conveyor belt, the end of the transmission shaft close to one end of the sample operating table is connected to a first bevel gear, a power transmission shaft vertically opposite to the first bevel gear is passed through the bottom of the first bevel gear, the top of the power transmission shaft is connected to a second bevel gear, the second bevel gear is meshed with the first bevel gear for transmission, the bottom end of the power transmission shaft is connected to a power transmission gear, and the end of the first transmission toothed belt away from the conveying transmission gear is sleeved on the outside of the power transmission gear.

[0022] As a preferred technical solution of the present application, the collecting baffle includes a closing baffle, one end of the closing baffle is connected to a rotating shaft, the bottom end of the rotating shaft is inserted into the inner side of the top surface of the working operating table, the outer wall of one end of the rotating shaft located inside the working operating table is sleeved with a first gear, a baffle starting motor is provided at the bottom of one side of the working operating table close to the first gear, a second gear is connected to the top of the output end of the baffle starting motor, a second transmission toothed belt is sleeved on the outer side of the second gear, the other end of the second transmission toothed belt is sleeved on the outer side of the first gear, a third gear is sleeved in the middle of the output end of the baffle starting motor, and a third transmission toothed belt is sleeved on the outer side of the third gear.

[0023] As a preferred technical solution of the present application, the positioning assembly includes a locking slider, which slides in the middle of the inner side of the sample operation table, and the locking slider is located on one side of the lifting transmission frame. The end of the locking slider away from the lifting transmission frame is connected to a transmission tooth plate, and a tooth plate driving shaft is provided on one side of the transmission tooth plate. The tooth plate driving shaft is vertically rotatably connected to the inner side of the sample operation table, and a fourth gear is sleeved at the bottom of the tooth plate driving shaft, and the end of the third transmission toothed belt away from the third gear is sleeved on the outside of the fourth gear. The outer wall of the upper side of the tooth plate driving shaft is sleeved with a tooth plate meshing gear, and the tooth plate meshing gear is meshed with the transmission tooth plate for transmission. A locking block telescopic box is provided in the middle of one side of the locking slider close to the lifting transmission frame, and a second spring is provided inside the locking block telescopic box. A positioning locking block is limitedly slid inside the end of the locking block telescopic box close to the lifting transmission frame, and the positioning locking block abuts against one end of the second spring. A locking groove is provided at equal intervals on one side of the outer wall of the lifting transmission frame close to the locking slider, and the second spring is inserted into the inner side of the locking groove, and the locking groove is relative to the number of sample chamber layers inside the cell storage bin.

[0024] The present invention provides a method for using a culture medium agitation control system for cell suspension culture, comprising the following steps:

[0025] S1: When placing the culture medium, the driving motor of the driving unit is started through the operating system, and the driving motor drives the cell storage bin to rise and the transfer platform to move at the same time. When the cell storage bin rises to the number of layers input by the system, the driving motor stops, the stirring mechanism is started, and the sample transfer plate is driven to rotate to the inside of the sample inlet, and the culture medium is placed between the sample transfer plates in the sample chamber through the sample inlet to complete the placement;

[0026] S2: When taking out the culture medium, the number of culture medium layers to be taken out is input into the system, and the system starts the drive motor to drive the cell storage bin to rise to the specified number of layers;

[0027] S3: The baffle start motor starts, driving the closed baffle to rotate to the upper side of the conveyor belt, and at the same time, the baffle start motor drives the locking slider to move, so that the positioning lock block is inserted into the inner side of the lock groove on the side of the lifting transmission frame;

[0028] S4: The stirring mechanism is started, the sample transfer plate rotates and drives the culture medium to the sample outlet, and the culture medium moves to the upper side of the conveyor belt through the sample outlet;

[0029] S5: The driving motor starts to drive the conveyor belt to move. At this time, the cell storage bin is locked due to the positioning lock block. The output shaft of the driving motor cannot drive the cell storage bin through the elastic drive of the driving sharp gear. The conveyor belt transports the culture medium to the surface of the closed baffle and slides to the upper side of the working table through the closed baffle.

[0030] S6: Turn off the drive motor, start the baffle motor to drive the closed baffle to reset, push the culture medium to the top surface of the workbench, and at the same time, the locking slider is reset and the lifting transmission frame is unlocked, and the drive motor is started to drive the cell storage bin to reset;

[0031] S7: The cell storage chamber is reset, the stirring motor is started, and the sample transfer plate is driven to rotate back and forth, pushing the culture medium to maintain the cell suspension culture.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] In the scheme of the present application: through the coordinated use of the cell storage bin inside the intelligent culture chamber and the transfer platform, the cell culture medium can be automatically taken out and transported to the outside, reducing the number of times the cell culture medium is opened and closed, and improving the safety of the culture environment inside the cell storage bin. At the same time, the stirring mechanism on the top of the cell storage bin can keep the culture medium placed inside the cell storage bin in a stirring state at all times, realizing the function of cell suspension culture, and the coordinated drive of the stirring mechanism and the cell drive unit can also accurately complete the removal of any number of layers of culture medium.

[0034] 1. The present invention provides a changing room inside the intelligent culture chamber, which can facilitate the staff to change their work clothes. There are two changing rooms, which can facilitate men and women to change at the same time, thereby improving work efficiency. The workroom inside the culture chamber is provided with a sample workroom and an operation workroom, which can reduce the entry of personnel into the sample room. At the same time, the two workrooms cooperate with the function of automatically taking out samples from the sample workroom and transporting them to the operation room, which can improve the protection safety inside the sample workroom.

[0035] 2. The present invention arranges the cell storage bin at the lower part of the sample operation table, which can improve the sealing effect inside the cell storage bin. At the same time, through the lifting and opening and closing of the cell storage bin and the chamber closing cover, the single opening and closing function of the storage space inside the cell storage bin can be realized, thereby improving the sealing of the individual closing effect inside each chamber.

[0036] 3. The present invention has a stirring mechanism on the top of the cell storage bin. During the process of storing cells in the cell storage bin, the stirring shaft can drive the sample transfer plate to slide back and forth, so that the culture medium placed between the sample transfer plates can always be in an active state during the placement process, thereby realizing the function of cell suspension culture. At the same time, the stirring mechanism also has the function of driving the culture medium to rotate by itself, and transporting the culture medium to the sample outlet, thereby completing the function of automatic sample delivery.

[0037] 4. The present invention provides a transfer table parallel to the top surface of the operating table behind the operating table inside the sample workroom and the operating workroom. After the stirring mechanism sends the culture medium out of the cell storage bin, the culture medium can be moved to one side of the operating workbench through the conveyor belt of the transfer table, thereby realizing the function of mechanized and automatic transfer of the culture medium and avoiding the steps of manual contact to open and close the cell storage bin. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A schematic diagram of the overall structure of a culture medium agitation control system and method for cell suspension culture provided by the present invention;

[0039] Figure 2 for Figure 1 The schematic diagram of the structure of the top section of the intelligent culture cabin shown;

[0040] Figure 3 for Figure 2 The schematic diagram of the structure of the workshop position section shown;

[0041] Figure 4 for Figure 3 The schematic diagram of the structure of the back of the interior of the workshop shown;

[0042] Figure 5 for Figure 4 The enlarged schematic diagram of the structural section of the front of the interior of the workshop is shown;

[0043] Figure 6 for Figure 5 A schematic diagram of the structure of the location of the cell storage compartment is shown;

[0044] Figure 7 for Figure 6 The schematic diagram of the structure of the cell storage compartment is shown as a cross-sectional exploded view;

[0045] Figure 8 for Figure 7 The structural sectional exploded schematic diagram of the stirring mechanism shown;

[0046] Fig. 9 for Figure 7 The schematic diagram of the structural decomposition of the driving part shown;

[0047] Fig.10for Fig. 9 The structural section exploded diagram of the driven main gear position shown;

[0048] Fig.11 for Figure 4 An enlarged schematic diagram of the transmission structure of the transmission platform position shown;

[0049] Fig.12 for Figure 5 A schematic cross-sectional view of the structure of the collecting baffle position shown;

[0050] Fig.13 for Fig.12 The driving structure of the collecting baffle is shown as an exploded and enlarged schematic diagram;

[0051] Fig.14 for Fig.13 An enlarged schematic diagram of the structure of the positioning component is shown.

[0052] Indicated in the figure:

[0053] 1. Intelligent culture cabin; 11. Sample workroom; 12. Operation workroom; 13. First changing room; 14. Second changing room; 15. Instrument room; 2. Operation table; 21. Sample operation table; 22. Work operation table; 23. Work table; 24. Bin closure cover; 25. Bin closure baffle; 26. Delivery outlet; 3. Cell storage bin; 31. Sample bin; 32. Sample inlet; 33. Sample outlet; 34. Lifting transmission frame; 35. Driving screw; 36. Transmission gear; 4. Stirring mechanism; 41. Stirring shaft; 42. Limiting combination cylinder; 43. Sample transfer plate; 44. Limiting combination ring; 45. Stirring motor; 5. Driving unit; 51. Driving motor; 52. Driving sharp gear; 53. Driven main gear; 54. Meshing wheel; 55. Elastic force Lock box; 56, first spring; 57, meshing tooth block; 58, conveying transmission gear; 59, first transmission belt; 6, transmission platform; 61, transmission shaft; 62, conveying roller; 63, transmission belt; 64, first bevel gear; 65, power transmission shaft; 66, second bevel gear; 67, power transmission gear; 7, collecting baffle; 71, closing baffle; 72, rotating shaft; 73, first gear; 74, baffle starting motor; 75, second gear; 76, second transmission belt; 77, third gear; 78, third transmission belt; 8, positioning assembly; 81, locking slider; 82, transmission tooth plate; 83, tooth plate drive shaft; 84, fourth gear; 85, tooth plate meshing gear; 86, lock block telescopic box; 87, positioning lock block; 88, second spring; 89, lock groove. DETAILED DESCRIPTION

[0054] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0055] As described in the background art, culture medium storage requires frequent opening of the culture space of the suspension culture chamber.

[0056] In order to solve this technical problem, the present invention provides a culture medium agitation control system and method for cell suspension culture, which is applied to the automation of storage culture medium retrieval in the suspension cell culture bin, reduces the number of times the suspension cell culture space is opened, and avoids the probability of bacteria entering the culture space.

[0057] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings.

[0058] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions in the embodiments may be combined with each other.

[0059] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0060] Example 1

[0061] Please refer to Figure 1-Figure 14 , a culture medium stirring control system for cell suspension culture, the system also includes an intelligent culture cabin 1, a sample workroom 11 and an operation workroom 12 are opened inside the intelligent culture cabin 1, the sample workroom 11 and the operation workroom 12 are opened adjacent to each other, a first changing room 13 and a second changing room 14 are also opened inside the intelligent culture cabin 1, the first changing room 13 and the second changing room 14 are opened adjacent to each other, an instrument room 15 is opened on the opposite side of the first changing room 13 and the second changing room 14, two groups of operating tables 2 are respectively located inside the sample workroom 11 and the operation workroom 12, and the operating tables 2 located inside the sample workroom 11 and the operation workroom 12 are placed in parallel.

[0062] The operating table 2 includes a sample operating table 21 and a working operating table 22. The sample operating table 21 is located on the inner side of the sample workroom 11, the working operating table 22 is located on the inner side of the operating workroom 12, the cell storage bin 3 is located on the inner side of the middle part of the sample operating table 21, the collecting baffle 7 is located on the side of the top surface of the working operating table 22 close to the operating workroom 12, the transmission table 6 is located on the inner side of the intelligent culture chamber 1 and is parallel to the top surfaces of the sample operating table 21 and the working operating table 22, a working table 23 is provided below the front of the sample operating table 21 and the working operating table 22, a bin body closing cover 24 is provided on the top surface of the sample operating table 21 located above the cell storage bin 3, a bin body closing cover 24 is connected to a bin chamber closing baffle 25 on the back side, and a conveying outlet 26 is opened between the bottom of the bin chamber closing baffle 25 and the top surface of the sample operating table 21.

[0063] A changing room is provided inside the intelligent culture chamber 1, which is convenient for the staff to change their work clothes. There are two changing rooms, which are convenient for men and women to change at the same time, thereby improving work efficiency. The workroom inside the culture chamber is provided with a sample workroom 11 and an operation workroom 12, which can reduce the entry of people into the sample room. At the same time, the two workrooms cooperate with the function of automatically taking out samples from the sample workroom 11 and transporting them to the operation room, which can improve the protection safety inside the sample workroom 11.

[0064] Example 2

[0065] The culture medium agitation control system for cell suspension culture provided in Example 1 is further optimized. Specifically, Figure 1-Figure 14 A sample chamber 31 is provided inside the cell storage bin 3, and the sample chamber 31 has multiple layers equidistantly provided from top to bottom. A sample inlet 32 ​​is provided on the front side of the cell storage bin 3, and the sample inlet 32 ​​is communicated with the inside of the sample chamber 31. A sample outlet 33 is provided on the back side of the cell storage bin 3, and the sample outlet 33 is communicated with the delivery outlet 26. Both ends of the cell storage bin 3 are connected to a lifting transmission frame 34, and a threaded driving screw 35 is passed through the lifting transmission frame 34, and a driving gear 36 is connected to the bottom end of the driving screw 35.

[0066] The driving part 5 includes a driving motor 51, which is located at the center just below the cell storage bin 3. The output end of the driving motor 51 is connected to a driving sharp gear 52. The top surface of the driving sharp gear 52 is provided with a driven main gear 53. The driven main gear 53 is meshed with the transmission gear 36 for transmission. The bottom surface of the driven main gear 53 is connected to a meshing wheel 54. The driving sharp gear 52 is embedded in the inner side of the meshing wheel 54. The position where the driving sharp gear 52 is connected at the center of the meshing wheel 54 is provided with a ring around the driving sharp gear 52. A distributed elastic lock box 55 is provided, and a first spring 56 is provided on the inner side of the elastic lock box 55. A meshing tooth block 57 is provided on the inner side of one side of the elastic lock box 55 close to the driving sharp gear 52 for limiting sliding. One end of the meshing tooth block 57 is located on the outer side of the elastic lock box 55 and meshes with the driving sharp gear 52. One end of the meshing tooth block 57 is located on the inner side of the elastic lock box 55 and abuts against the end of the first spring 56. A conveying transmission gear 58 is connected to the middle of the output end of the driving motor 51, and a first transmission toothed belt 59 is fitted on the outer side of the conveying transmission gear 58.

[0067] The cell storage bin 3 is arranged at the lower part of the sample operation table 21, which can improve the sealing effect inside the cell storage bin 3. At the same time, through the lifting and opening and closing of the cell storage bin 3 and the cooperation with the bin closing cover, the single opening and closing function of the internal storage space of the cell storage bin 3 can be realized, thereby improving the sealing of the individual closing effect inside each bin.

[0068] Example 3

[0069] A culture medium agitation control system for cell suspension culture provided in Example 1 or 2 is further optimized. Specifically, Figure 1-Figure 14 As shown, the stirring mechanism 4 includes a stirring shaft 41, and the stirring shaft 41 is vertically arranged at the center of the cell storage bin 3. The outer wall of the stirring shaft 41 is located inside the sample chamber 31 and is fitted with a limiting combination tube 42. Symmetrical sample transfer plates 43 are connected to the outer walls of the limiting combination tube 42 on both sides. A limiting combination ring 44 is provided at the position where the limiting combination tube 42 is connected inside the sample chamber 31. The upper and lower surfaces of the limiting combination tube 42 are fitted on the outside of the limiting combination ring 44. A stirring motor 45 is provided on the top surface of the cell storage bin 3. The output end of the stirring motor 45 is drivingly connected to the stirring shaft 41.

[0070] A stirring mechanism 4 is provided on the top of the cell storage bin 3. During the process of storing and placing cells in the cell storage bin 3, the stirring shaft 41 can drive the sample transfer plate 43 to slide back and forth, so that the culture medium placed between the sample transfer plates 43 can always be in an active state during the placement process, thereby realizing the function of cell suspension culture. At the same time, the stirring mechanism 4 also has the function of being able to drive the culture medium to rotate by itself, and transport the culture medium to the sample outlet 33, thereby completing the function of automatic sample delivery.

[0071] Example 4

[0072] A culture medium agitation control system for cell suspension culture provided in Example 1, 2 or 3 is further optimized, specifically, as follows: Figure 1-Figure 14 As shown, the transmission platform 6 includes two groups of transmission shafts 61, and the transmission shafts 61 are rotatably connected to the two sides of the inside of the intelligent culture cabin 1. The outer side of the end of the transmission shaft 61 close to the sample operation table 21 and the work operation table 22 is sleeved with a conveying roller 62, and the outer side of the conveying roller 62 is sleeved with a conveying belt 63. The end of the transmission shaft 61 close to one end of the sample operation table 21 is connected to a first bevel gear 64, and a power transmission shaft 65 perpendicular to it is penetrated below the first bevel gear 64. The top of the power transmission shaft 65 is connected to a second bevel gear 66, and the second bevel gear 66 is meshed with the first bevel gear 64 for transmission. The bottom end of the power transmission shaft 65 is connected to a power transmission gear 67, and the end of the first transmission toothed belt 59 away from the conveying transmission gear 58 is sleeved on the outer side of the power transmission gear 67.

[0073] A transfer table 6 parallel to the top surface of the operation table 2 is provided behind the operation table 2 inside the sample workroom 11 and the operation workroom 12. After the stirring mechanism 4 sends the culture medium out of the cell storage bin 3, the culture medium is moved to one side of the operation workbench 23 via the conveyor belt 63 of the transfer table 6, thereby realizing the function of mechanized and automatic transfer of the culture medium and avoiding the step of manually touching and opening and closing the cell storage bin 3.

[0074] Example 5

[0075] A culture medium agitation control system for cell suspension culture provided in Examples 1-4 is further optimized. Specifically, Figure 1-Figure 14 As shown, the collecting baffle 7 includes a closing baffle 71, one end of the closing baffle 71 is connected to a rotating shaft 72, the bottom end of the rotating shaft 72 is inserted into the inner side of the top surface of the working table 22, and the outer wall of one end of the rotating shaft 72 located inside the working table 22 is sleeved with a first gear 73, and a baffle starting motor 74 is provided at the bottom of one side of the working table 22 close to the first gear 73, a second gear 75 is connected to the top of the output end of the baffle starting motor 74, a second transmission toothed belt 76 is sleeved on the outer side of the second gear 75, and the other end of the second transmission toothed belt 76 is sleeved on the outer side of the first gear 73, a third gear 77 is sleeved in the middle of the output end of the baffle starting motor 74, and a third transmission toothed belt 78 is sleeved on the outer side of the third gear 77.

[0076] A collecting baffle 7 is provided on the top surface of the operating table 2 in the operating workroom 12. The collecting baffle 7 is driven to rotate by the baffle starting motor 74 inside the operating table 2. During the process of the culture medium being transported by the transfer table 6, the moving path of the culture medium can be changed, and the culture medium can be transferred to the upper side of the working table 22, thereby realizing the automatic removal of the culture medium and the transfer of the culture medium to the upper side of the working table 22.

[0077] Example 6

[0078] A culture medium agitation control system for cell suspension culture provided in Examples 1-5 is further optimized. Specifically, Figure 1-Figure 14 As shown, the positioning assembly 8 includes a locking slider 81, which slides in the middle of the inner side of the sample operating table 21, and the locking slider 81 is located on one side of the lifting transmission frame 34. The end of the locking slider 81 away from the lifting transmission frame 34 is connected to a transmission tooth plate 82, and a tooth plate driving shaft 83 is provided on one side of the transmission tooth plate 82. The tooth plate driving shaft 83 is vertically rotatably connected to the inner side of the sample operating table 21, and a fourth gear 84 is sleeved on the bottom of the tooth plate driving shaft 83. The end of the third transmission toothed belt 78 away from the third gear 77 is sleeved on the outside of the fourth gear 84, and the upper outer wall of the tooth plate driving shaft 83 is sleeved with a tooth plate meshing Gear 85, the toothed plate meshing gear 85 is meshed with the transmission toothed plate 82 for transmission, a locking block telescopic box 86 is provided in the middle of one side of the locking slide block 81 close to the lifting transmission frame 34, a second spring 88 is provided inside the locking block telescopic box 86, a positioning locking block 87 is provided on the inner side of one end of the locking block telescopic box 86 close to the lifting transmission frame 34 for limiting sliding, the positioning locking block 87 is in contact with one end of the second spring 88, and a locking groove 89 equidistantly provided on the outer wall of the lifting transmission frame 34 close to the locking slide block 81, the second spring 88 is inserted inside the locking groove 89, and the locking groove 89 is opposite to the number of layers of the sample chamber 31 inside the cell storage bin 3.

[0079] A positioning component 8 is provided inside the sample operation table 21 and is limitedly engaged with the cell storage bin 3. When the driving unit 5 drives the cell storage bin 3 to move upward, the sample chamber 31 of a specified number of layers can be positioned, thereby cooperating with the elastic transmission of the elastic lock box 55 and the driving pointed gear 52 inside the driving unit 5 to realize the function of the driving unit 5 to drive the transmission table 6 alone, and the positioning component 8 is connected with the baffle starting motor 74 of the collection baffle 7. When the collection baffle 7 is driven to rotate, the positioning component 8 can be driven synchronously to complete the positioning of the specified number of layers of the cell storage bin 3, and automatically release the positioning of the cell storage bin 3 after the culture medium is collected, so that the cell storage bin 3 can continue to be reset by the driving unit 5.

[0080] See also Figure 1-Figure 14, an embodiment of the present invention provides a method for using a culture medium agitation control system for cell suspension culture, the method for using a culture medium agitation control system for cell suspension culture comprising the following steps:

[0081] S1: When placing the culture medium, the driving motor 51 of the driving unit 5 is started through the operating system, and the driving motor 51 drives the cell storage bin 3 to rise and the transfer platform 6 to move at the same time. When the cell storage bin 3 rises to the number of layers input by the system, the driving motor 51 stops, and the stirring mechanism 4 is started to drive the sample transfer plate 43 to rotate to the inside of the sample inlet 32, and the culture medium is placed between the sample transfer plates 43 in the sample chamber 31 through the sample inlet 32, and the placement is completed;

[0082] S2: When taking out the culture medium, the number of culture medium layers to be taken out is input to the system, and the system starts the drive motor 51 to drive the cell storage bin 3 to rise to the specified number of layers;

[0083] S3: The baffle start motor 74 is started to drive the closing baffle 71 to rotate to the upper side of the conveyor belt 63. At the same time, the baffle start motor 74 drives the locking slider 81 to move so that the positioning lock block 87 is inserted into the inner side of the lock slot 89 on the side of the lifting transmission frame 34.

[0084] S4: The stirring mechanism 4 is started, the sample transfer plate 43 rotates to drive the culture medium to the sample outlet 33, and the culture medium moves to the upper side of the conveyor belt 63 through the sample outlet 33;

[0085] S5: The driving motor 51 starts to drive the conveyor belt 63 to move. At this time, the cell storage bin 3 is locked due to the positioning lock block 87. The output shaft of the driving motor 51 cannot drive the cell storage bin 3 through the elastic drive of the driving sharp gear 52. The conveyor belt 63 transports the culture medium to the surface of the closed baffle 71, and slides to the upper side of the working table 22 through the closed baffle 71.

[0086] S6: The driving motor 51 is turned off, and the baffle starting motor 74 is started to drive the closing baffle 71 to reset, pushing the culture medium to the top surface of the working table 22, and at the same time, the locking slider 81 is reset and the lifting transmission frame 34 is unlocked, and the driving motor 51 is started to drive the cell storage bin 3 to reset;

[0087] S7: The cell storage chamber 3 is reset, and the stirring motor 45 is started to drive the sample transfer plate 43 to reciprocate, push the culture medium, and maintain the suspension culture of the cells.

[0088] The use process of a culture medium agitation control system for cell suspension culture provided by the present invention is as follows:

[0089] The culture medium placement instruction is input to the system, and the number of culture medium placement layers is input, after which the system starts the drive motor 51, and the drive sharp gear 52 and the conveying transmission gear 58 connected to the output end of the drive motor 51 rotate synchronously.

[0090] The rotation of the driving pointed gear 52 will apply a rotational thrust to the multiple groups of elastic lock boxes 55 meshing with it. The elastic lock box 55 transmits the rotational force to the elastic lock box 55 through the meshing tooth block 57 through the first spring 56. The elastic lock box 55 transmits the rotational force to the driven main gear 53. The driven main gear 53 rotates to drive the two transmission gears 36 meshing with it. The rotation of the transmission gear 36 drives the driving screw 35 to rotate synchronously. The rotation of the driving screw 35 can drive the lifting transmission frame 34 on its outer side to move upward. The upward movement of the lifting transmission frame 34 drives the cell storage bin 3 to rise. When the cell storage bin 3 rises to the number of layers specified by the system, the driving motor 51 stops.

[0091] The rotation of the conveying transmission gear 58 will drive the power transmission gear 67 to rotate through the first transmission belt 59. The rotation of the dynamic transmission gear 36 will drive the power transmission shaft 65 to rotate. The rotation of the power transmission shaft 65 will drive the second bevel gear 66 to rotate. The rotation of the second bevel gear 66 will drive the first bevel gear 64 to rotate. The rotation of the first bevel gear 64 will drive the transmission shaft 61 to rotate. The rotation of the transmission shaft 61 will drive the conveying roller 62 to rotate. The rotation of the conveying roller 62 will drive the transmission belt 63 to move.

[0092] After the cell storage bin 3 moves to the specified number of layers, the specified number of layers will be parallel to the top surface of the sample operating table 21, and the sample outlet 33 will be parallel to the conveying outlet 26. The system starts the stirring mechanism 4 and the collection baffle 7 to rotate, and the stirring motor 45 starts to drive the stirring shaft 41 to rotate. The rotation of the stirring shaft 41 drives the limiting combination cylinder 42 and the sample transfer plate 43 to rotate. The rotation of the sample transfer plate 43 drives the culture medium placed in the middle to rotate. When the culture medium rotates to the sample outlet 33, the culture medium will be cut off by the chamber area at the position of the sample outlet 33, allowing the culture medium to fall to the upper side of the conveyor belt 63.

[0093] After the collecting baffle 7 is started, the second gear 75 and the third gear 77 connected to the output end of the baffle starting motor 74 will rotate synchronously. The rotation of the second gear 75 will drive the first gear 73 to rotate through the second transmission belt 76. The rotation of the first gear 73 will drive the rotating shaft 72 to rotate. The rotation of the rotating shaft 72 will cause the closed baffle 71 connected to it to rotate. After the closed baffle 71 rotates, the other end of the closed baffle 71 will slide to the upper side of the conveyor belt 63, forming an oblique barrier to the conveyor belt 63 as a whole.

[0094] The rotation of the third gear 77 will drive the fourth gear 84 to rotate through the third transmission toothed belt 78, and the rotation of the fourth gear 84 will drive the gear plate driving shaft 83 to rotate. The rotation of the gear plate driving shaft 83 will drive the gear plate meshing gear 85 coaxially connected thereto to rotate. The rotation of the gear plate meshing gear 85 will drive the transmission gear plate 82 to move. The movement of the transmission gear plate 82 will drive the locking slider 81 to move toward one side of the lifting transmission frame 34. When the locking slider 81 moves, the locking block telescopic box 86 connected to its end will drive the positioning locking block 87 to move synchronously. When the positioning locking block 87 moves to the inner side of the locking groove 89 on the outer wall of the lifting transmission frame 34, the lifting and lowering of the cell storage bin 3 can be blocked and positioned by the positioning locking block 87.

[0095] It should be noted that the lock block telescopic box 86 is elastically telescopically connected to the positioning lock block 87 through the second spring 88, which can avoid driving conflict between the rotation drive of the positioning component 8 and the collecting baffle 7, allowing the closing baffle 71 to rotate to the maximum distance without affecting the locking of the lifting transmission frame 34 by the positioning lock block 87.

[0096] After the cell storage bin 3 is locked, the system starts the drive motor 51 to drive the drive sharp gear 52 and the conveying transmission gear 58. Since the cell storage bin 3 is locked by the positioning lock block 87, the rotational driving force of the drive sharp gear 52 will push the meshing tooth block 57 inside the elastic lock box 55 to retract to the inside of the elastic lock box 55, thereby absorbing the rotational force of the drive sharp gear 52 and keeping the drive motor 51 able to continue to rotate and drive.

[0097] After the conveying transmission gear 58 is driven, the conveyor belt 63 will move toward the position of the closed baffle 71. At the same time, since the culture medium has been pushed to the upper side of the conveyor belt 63 by the stirring mechanism 4 at this time, the culture medium will be driven to the position of the closed baffle 71 by the conveyor belt 63. Under the transportation of the conveyor belt 63, the culture medium will pass through the oblique obstruction of the closed baffle 71 and the push of the bottom conveyor belt 63, and the culture medium will move to the position of the working table 22.

[0098] After the culture medium moves to the inside of the closed baffle 71, the system starts the baffle start motor 74 to rotate in the reverse direction, driving the closed baffle 71 to move the culture medium to the upper side of the workbench 22, thereby completing the taking of the culture medium.

[0099] When the closed baffle 71 is reset, the baffle starting motor 74 will simultaneously drive the locking slider 81 to reset, separate the positioning lock block 87 from the lifting transmission frame 34, and release the drive disconnection between the cell storage bin 3 and the driving unit 5. At this time, the driving motor 51 is started to move the cell storage bin 3 down to the inside of the sample operating table 21, and the stirring motor 45 is started to drive the sample transfer plate 43 to drive the culture medium to reciprocate inside the sample chamber 31, so that the cells can be placed in a suspended culture state.

[0100] When placing the culture medium, it is only necessary to start the drive motor 51 to raise the cell storage bin 3 to the layer number required, start the stirring mechanism 4 to rotate the sample transfer plate 43 to the position of the sample inlet 32, place the culture medium into the inner side of the sample chamber 31 through the sample inlet 32, start the drive motor 51 to reset the cell storage bin 3, and start the stirring mechanism 4 to stir the culture medium.

[0101] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0102] Obviously, the embodiments described above are only some embodiments of the present invention, rather than all embodiments. The preferred embodiments of the present invention are given in the accompanying drawings, but they do not limit the patent scope of the present invention. The present invention can be implemented in many different forms. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. Although the present invention has been described in detail with reference to the aforementioned embodiments, for those skilled in the art, it is still possible to modify the technical solutions recorded in the aforementioned specific embodiments, or to perform equivalent replacements for some of the technical features therein. Any equivalent structure made using the contents of the specification and drawings of the present invention, directly or indirectly used in other related technical fields, is also within the scope of patent protection of the present invention.

Claims

1. A culture medium agitation control system for cell suspension culture, characterized in that: The system comprises: An operating table (2), wherein the operating table (2) is provided with two groups, and the operating table (2) is used for placing culture medium suspension culture and culture medium culture operation; A cell storage bin (3), the cell storage bin (3) being located inside the middle of a group of operating tables (2), the cell storage bin (3) being lifted and slid inside the operating tables (2), and the cell storage bin (3) being used to store multiple culture media in layers and in a sealed manner; A stirring mechanism (4), the stirring mechanism (4) is connected to the center of the top surface of the cell storage bin (3), the output end of the stirring mechanism (4) is arranged inside the cell storage bin (3), and the output end of the stirring mechanism (4) can drive the culture medium placed in layers inside the cell storage bin (3) to reciprocate and rotate; A driving unit (5), the driving unit (5) being located below the cell storage bin (3), and an output end of the driving unit (5) driving the cell storage bin (3) to vertically ascend and descend; A transfer platform (6), the transfer platform (6) is located on the upper back side of the operating platform (2), the top surface of the transfer platform (6) is parallel to the top surface of the operating platform (2), one end of the transfer platform (6) is connected to the driving unit (5), and the transfer platform (6) is used to transport the culture medium sent out from the cell storage bin; A collecting baffle (7), wherein the collecting baffle (7) is rotatably connected to a position on the top of another set of operating tables (2) close to the transmission table (6), and the collecting baffle (7) can block the culture medium during the transportation process in a rotating state, thereby changing the path of the culture medium and transporting it to the top surface of the operating table (2); A positioning component (8), wherein the positioning component (8) is located on one side of the cell storage bin (3), the positioning component (8) is plugged into the inner side of the outer wall of the cell storage bin (3) for positioning, the end of the positioning component (8) facing away from the cell storage bin (3) is transmission-connected with the collecting baffle (7), the positioning component (8) is synchronously driven to be plugged into the cell storage bin (3) to position the lifting position of the cell storage bin (3) when the collecting baffle (7) is driven, and is used to disconnect the transmission relationship between the driving unit (5) and the cell storage bin (3), and maintain the continuous output of the driving unit (5) to the transmission platform (6) when the lifting layer number of the cell storage bin (3) is positioned.

2. A culture medium agitation control system for cell suspension culture according to claim 1, characterized in that: The system further comprises an intelligent culture cabin (1), wherein a sample workroom (11) and an operation workroom (12) are provided inside the intelligent culture cabin (1), wherein the sample workroom (11) and the operation workroom (12) are provided adjacent to each other, wherein a first changing room (13) and a second changing room (14) are provided inside the intelligent culture cabin (1), wherein the first changing room (13) and the second changing room (14) are provided adjacent to each other, wherein an instrument room (15) is provided on the opposite side of the first changing room (13) and the second changing room (14), wherein two groups of operating tables (2) are respectively located inside the sample workroom (11) and the operation workroom (12), wherein the operating tables (2) located inside the sample workroom (11) and the operation workroom (12) are placed in parallel.

3. A culture medium agitation control system for cell suspension culture according to claim 2, characterized in that: The operating table (2) comprises a sample operating table (21) and a working operating table (22), wherein the sample operating table (21) is located inside the sample working room (11), the working operating table (22) is located inside the operating working room (12), the cell storage bin (3) is located inside the middle of the sample operating table (21), the collection baffle (7) is located on the top surface of the working operating table (22) close to the operating working room (12), and the transmission table (6) is located inside the intelligent culture cabin (1). A workbench (23) is provided below the front of the sample operation table (21) and the work operation table (22) and is parallel to the top surface of the sample operation table (21) and the work operation table (22). A chamber closing cover (24) is provided on the top surface of the sample operation table (21) and is located above the cell storage chamber (3). A chamber closing baffle (25) is connected to the back of the chamber closing cover (24). A delivery outlet (26) is provided between the bottom of the chamber closing baffle (25) and the top surface of the sample operation table (21).

4. A culture medium agitation control system for cell suspension culture according to claim 3, characterized in that: The cell storage bin (3) has a sample chamber (31) disposed therein, and the sample chamber (31) has multiple layers equidistantly disposed therein from top to bottom. A sample inlet (32) is disposed on one side of the front of the cell storage bin (3), and the sample inlet (32) is communicated with the interior of the sample chamber (31). A sample outlet (33) is disposed on the back of the cell storage bin (3), and the sample outlet (33) is communicated with the delivery outlet (26). Both ends of the cell storage bin (3) are connected to a lifting transmission frame (34), and a threaded drive screw (35) is disposed therein, and a drive gear (36) is connected to the bottom end of the drive screw (35).

5. A culture medium agitation control system for cell suspension culture according to claim 4, characterized in that: The stirring mechanism (4) comprises a stirring shaft (41), the stirring shaft (41) is vertically arranged at the center of the cell storage bin (3), the outer wall of the stirring shaft (41) is located inside the sample chamber (31) and is fitted with a limiting combination cylinder (42), both sides of the outer wall of the limiting combination cylinder (42) are connected to symmetrical sample transfer plates (43), a limiting combination ring (44) is provided at a position inside the sample chamber (31) connected to the limiting combination cylinder (42), the upper and lower surfaces of the limiting combination cylinder (42) are fitted on the outside of the limiting combination ring (44), and a stirring motor (45) is provided on the top surface of the cell storage bin (3), and the output end of the stirring motor (45) is drivingly connected to the stirring shaft (41).

6. A culture medium agitation control system for cell suspension culture according to claim 5, characterized in that: The driving unit (5) comprises a driving motor (51), the driving motor (51) is located at the center just below the cell storage bin (3), the output end of the driving motor (51) is connected to a driving sharp gear (52), the top surface of the driving sharp gear (52) is provided with a driven main gear (53), the driven main gear (53) is meshed with the transmission gear (36) for transmission, the bottom surface of the driven main gear (53) is connected to a meshing wheel (54), the driving sharp gear (52) is embedded inside the meshing wheel (54), and the center of the meshing wheel (54) connected to the driving sharp gear (52) is provided with a ring around the driving sharp gear (52) An elastic lock box (55) is arranged in a shape of a spring lock box (55), a first spring (56) is arranged inside the elastic lock box (55), a meshing tooth block (57) is provided on the inner side of a surface of the elastic lock box (55) close to the driving sharp gear (52) for limiting sliding, one end of the meshing tooth block (57) located on the outer side of the elastic lock box (55) is meshed with the driving sharp gear (52), one end of the meshing tooth block (57) located on the inner side of the elastic lock box (55) is abutted against the end of the first spring (56), a conveying transmission gear (58) is connected to the middle of the output end of the driving motor (51), and a first transmission toothed belt (59) is sleeved on the outer side of the conveying transmission gear (58).

7. A culture medium agitation control system for cell suspension culture according to claim 6, characterized in that: The transmission platform (6) comprises two groups of transmission shafts (61), the transmission shafts (61) are rotatably connected to the two sides of the inside of the intelligent culture cabin (1), the outer side of one end of the transmission shaft (61) close to the sample operation platform (21) and the work operation platform (22) is sleeved with a conveying roller (62), the outer side of the conveying roller (62) is sleeved with a conveying belt (63), the end of the transmission shaft (61) close to one end of the sample operation platform (21) is connected with a first bevel gear (64), a power transmission shaft (65) perpendicularly opposite to the first bevel gear (64) is passed through below the first bevel gear (64), the top end of the power transmission shaft (65) is connected with a second bevel gear (66), the second bevel gear (66) is meshed with the first bevel gear (64) for transmission, the bottom end of the power transmission shaft (65) is connected with a power transmission gear (67), and the end of the first transmission toothed belt (59) away from the conveying transmission gear (58) is sleeved on the outer side of the power transmission gear (67).

8. A culture medium agitation control system for cell suspension culture according to claim 7, characterized in that: The collecting baffle (7) comprises a closing baffle (71), one end of the closing baffle (71) is connected to a rotating shaft (72), the bottom end of the rotating shaft (72) is arranged on the inner side of the top surface of the working platform (22), the outer wall of one end of the rotating shaft (72) located inside the working platform (22) is sleeved with a first gear (73), a baffle starting motor (74) is arranged at the bottom of one side of the working platform (22) close to the first gear (73), the top of the output end of the baffle starting motor (74) is connected to a second gear (75), the outer side of the second gear (75) is sleeved with a second transmission toothed belt (76), the other end of the second transmission toothed belt (76) is sleeved on the outer side of the first gear (73), the middle part of the output end of the baffle starting motor (74) is sleeved with a third gear (77), the outer side of the third gear (77) is sleeved with a third transmission toothed belt (78).

9. A culture medium agitation control system for cell suspension culture according to claim 8, characterized in that: The positioning assembly (8) comprises a locking slider (81), wherein the locking slider (81) slides in the middle of the inner side of the sample operation table (21), and the locking slider (81) is located on one side of the lifting transmission frame (34). The end of the locking slider (81) away from the lifting transmission frame (34) is connected to a transmission tooth plate (82), and a tooth plate driving shaft (83) is provided on one side of the transmission tooth plate (82). The tooth plate driving shaft (83) is vertically rotatably connected to the inner side of the sample operation table (21), and a fourth gear (84) is sleeved on the bottom of the tooth plate driving shaft (83), and the end of the third transmission toothed belt (78) away from the third gear (77) is sleeved on the outer side of the fourth gear (84), and the upper outer wall of the tooth plate driving shaft (83) is sleeved with a tooth plate meshing gear ( 85), the tooth plate meshing gear (85) is meshed with the transmission tooth plate (82) for transmission, a locking block telescopic box (86) is provided in the middle of one side of the locking slide block (81) close to the lifting transmission frame (34), a second spring (88) is provided inside the locking block telescopic box (86), a positioning locking block (87) is provided inside one end of the locking block telescopic box (86) close to the lifting transmission frame (34) for limiting sliding, the positioning locking block (87) is in contact with one end of the second spring (88), a locking groove (89) is provided at equal intervals on one side of the outer wall of the lifting transmission frame (34) close to the locking slide block (81), the second spring (88) is inserted into the inner side of the locking groove (89), and the locking groove (89) is opposite to the number of layers of the sample chamber (31) inside the cell storage bin (3).

10. A method for using a culture medium agitation control system for cell suspension culture according to claims 1-9, characterized in that: The method for using the culture medium agitation control system for cell suspension culture comprises the following steps: S1: When placing the culture medium, the driving motor (51) of the driving unit (5) is started through the operating system, and the driving motor (51) simultaneously drives the cell storage bin (3) to rise and the transfer platform (6) to move. When the cell storage bin (3) rises to the number of layers input by the system, the driving motor (51) stops, the stirring mechanism (4) is started, and the sample transfer plate (43) is driven to rotate to the inside of the sample inlet (32), and the culture medium is placed between the sample transfer plates (43) in the sample chamber (31) through the sample inlet (32), thereby completing the placement; S2: When taking out the culture medium, the number of culture medium layers to be taken out is input into the system, and the system starts the drive motor (51) to drive the cell storage bin (3) to rise to the specified number of layers; S3: The baffle start motor (74) is started to drive the closing baffle (71) to rotate to the upper side of the conveyor belt (63), and at the same time, the baffle start motor (74) drives the locking slider (81) to move, so that the positioning lock block (87) is inserted into the inner side of the lock groove (89) on the side of the lifting transmission frame (34); S4: the stirring mechanism (4) is started, the sample transfer plate (43) rotates to drive the culture medium to the sample outlet (33), and the culture medium moves to the upper side of the conveyor belt (63) through the sample outlet (33); S5: The driving motor (51) starts to drive the conveyor belt (63) to move. At this time, the cell storage bin (3) is locked due to the positioning lock block (87). The output shaft of the driving motor (51) cannot drive the cell storage bin (3) through the elastic drive of the driving pointed gear (52). The conveyor belt (63) transports the culture medium to the surface of the closed baffle (71), and slides to the upper side of the working table (22) through the closed baffle (71); S6: The drive motor (51) is turned off, the baffle start motor (74) is started to drive the closed baffle (71) to reset, and the culture medium is pushed to the top surface of the working table (22). At the same time, the locking slider (81) is reset and the lifting transmission frame (34) is unlocked, and the drive motor (51) is started to drive the cell storage bin (3) to reset; S7: The cell storage chamber (3) is reset, and the stirring motor (45) is started to drive the sample transfer plate (43) to reciprocate, push the culture medium, and maintain the suspended culture of the cells.

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