Device for screening noise-stress-resistant large yellow croakers based on behavior response

Abstract

The invention provides a device for screening noise stress resistant pseudosciaena crocea based on behavior response. The device comprises a culture system, a behavior monitoring system and a noise stimulation system, the culture system comprises a circulating water device arranged at the water tank, an oxygenation pump, a temperature sensing control switch and a heating rod; the behavior monitoring system comprises a monitor above the water tank and an underwater temperature-sensing depth-sensing monitor at the water tank; the noise stimulation system comprises a control module, a sound stimulation audio synthesis module, a sound stimulation signal release module and an underwater acoustic characteristic acquisition and analysis module. The underwater acoustic characteristic acquisition and analysis module comprises a hydrophone, a power amplifier and an AD converter; the screening device stimulates the large yellow croakers in the water tank through noise directionally played by the noise stimulation system, and the behavior monitoring system analyzes state changes of the large yellow croakers under noise stimulation so as to screen out the large yellow croakers resistant to noise stress. The method can be used for researching the behavior response of the pseudosciaena crocea under different noise stresses, and can be used for screening the pseudosciaena crocea individuals tolerant to noise stresses.

Description

Technical Field

[0001] The invention relates to the field of fish behavior and passive acoustic technology, in particular to a device for screening large yellow croakers resistant to noise stress based on behavioral responses. Background Art

[0002] As a large aquaculture country, my country has abundant wind resources along the southeast coast. The deep integration and development of marine aquaculture and offshore wind power will become a new industrial model for economical and intensive use of the sea, leading the industry towards a greener and more sustainable future. At present, large yellow croaker (Larimichthys crocea) has become the largest marine aquaculture fish in my country. The waters of Fujian and Zhejiang are the main large yellow croaker aquaculture areas in my country, accounting for about 85.49% of the national large yellow croaker aquaculture output. The main auditory organs of large yellow croaker are the inner ear, lateral line and swim bladder. It includes semicircular canals (anterior semicircular canals, posterior semicircular canals, horizontal semicircular canals) and sac-like structures containing otoliths (oval sac, saccule, bottle sac), which cover the sensory epithelium, and the developed auditory structure is directly related to its vocalization function. The urgent problem to be solved in the aquaculture of large yellow croaker in the wind-fishing integration model is the stress problem caused by the sensitivity of large yellow croaker to sound. Therefore, with regard to the research direction of large yellow croaker's tolerance to noise stimulation, it is of great significance to design and develop a device that can screen large yellow croaker individuals that can tolerate noise stress.

[0003] The scheme adopted by the present invention can screen out large yellow croaker individuals that are tolerant to noise stress, which can provide support for genome-wide association study and transcriptome analysis to screen large yellow croaker auditory sensitivity genes, and further promote the development and innovation of wind-fishing integrated breeding model. Summary of the invention

[0004] The present invention proposes a device for screening large yellow croakers resistant to noise stress based on behavioral responses, which can study the behavioral responses of large yellow croakers under different noise stresses and screen large yellow croaker individuals that can tolerate a certain degree of noise stress.

[0005] The present invention adopts the following technical solutions.

[0006] A device for screening large yellow croakers resistant to noise stress based on behavioral response, the screening device comprising a breeding system, a behavior monitoring system and a noise stimulation system; the breeding system comprising a circulating water device, an aeration pump, a temperature sensing control switch and a heating rod arranged at a water tank; the behavior monitoring system comprising a monitor above the water tank and an underwater temperature and depth sensing monitor at the water tank; the noise stimulation system comprising a control module, an acoustic stimulation audio synthesis module comprising a function generator and a power amplifier, an acoustic stimulation signal release module comprising an underwater speaker, and also comprising an underwater acoustic characteristic acquisition and analysis module arranged at different areas of the screening device; the underwater acoustic characteristic acquisition and analysis module comprising a hydrophone, a power amplifier and an AD converter; when the screening device is working, the environmental parameters of the water tank where the large yellow croaker is located are first adjusted through the breeding system, and then the large yellow croaker in the water tank is stimulated with noise directed by the noise stimulation system, and the state changes of the large yellow croaker under the noise stimulation are analyzed by the behavior monitoring system, so as to screen out large yellow croakers resistant to noise stress.

[0007] The power amplifier (1) is connected to the function generator (2) via a data transmission line, and the power amplifier (1) is connected to the underwater speaker (3) and the computer of the control module.

[0008] The water tank comprises a resting water tank (14) and a screening noise stress resistance test water tank (4); the underwater speaker is attached to and fixed to the water tank wall of the screening noise stress resistance test water tank (4); the screening noise stress resistance test water tank comprises a temporary holding area (5) and a behavior screening area (7) separated by a movable partition (6); the partition can be removed from the screening noise stress resistance test water tank.

[0009] The underwater speaker is placed on the wall of the tank in the temporary breeding area, and its sound emission direction is toward the behavior screening area; the bottom of the behavior screening area (7) has area partitions and letter marks (11), a hydrophone (10) is fixed at the center of each separated area in the behavior screening area (7), and a high-definition camera (9) is fixed directly above the behavior screening area (7).

[0010] The noise stress resistance screening test water tank (4) is connected to the static water tank (14) via a silencing pipe (13). A control water valve (12) is installed on the silencing pipe (13). When the noise stimulation system starts the noise stress operation, the control water valve (12) is closed.

[0011] A first shockproof and sound-absorbing pad (8) is installed at the bottom of the water tank for screening noise stress resistance experiment; a second shockproof and sound-absorbing pad (17) is installed at the bottom of the resting water tank, and sound-absorbing cotton (16) is installed on the side; the first shockproof and sound-absorbing pad and the second shockproof and sound-absorbing pad are made of the same material.

[0012] An underwater temperature and depth sensing monitor (15) is provided in the static water tank, and the static water tank is connected to a constant temperature water tank (18). A temperature sensing control switch (19) is fixed in the constant temperature water tank (18), and the temperature sensing control switch (19) is connected to a heating rod (20) for controlling the water temperature of the entire screening device;

[0013] The constant temperature water tank (17) is connected to a circulating water system (21), and a filter material (22) and an oxygen pump (23) for optimizing water quality are placed in the circulating water system (21), so as to ensure that the water quality and dissolved oxygen content of the entire screening device meet the required standards.

[0014] Each of the large yellow croakers used for screening is provided with a label that can be identified by a behavior monitoring system, so as to distinguish different fish.

[0015] The method for using the screening device specifically comprises the following steps:

[0016] Step S1, screening the temporary rearing area (5) in the noise stress resistance experimental water tank (4) for temporary rearing of experimental fish before the experiment begins, selecting 60 large yellow croakers with healthy and intact body surfaces to be temporarily reared in the temporary rearing area for 3 days, during which the control water valve (12), the constant temperature water tank (18), the temperature sensing control switch (19), the heating rod (20) and the circulating water system (20) are all in an open state to ensure that the device can provide suitable environmental conditions for the large yellow croakers;

[0017] Step S2, before the noise stress begins, the circulating water system (21) and the control water valve (12) are closed to prevent the background noise generated by them from affecting the state of the large yellow croaker; after the circulating water system (21) and the control water valve (12) are closed, the sound release parameters are set according to the auditory characteristics of the large yellow croaker and the stimulation requirements, first ensuring that the power amplifier (1), the function generator (2) and the underwater speaker (3) function normally, then turning on the hydrophone (10) and the high-definition camera (9), and monitoring the underwater noise and video changes in the noise stress resistance screening test tank (4);

[0018] Step S3, first round of screening: quickly and rapidly remove the partition (6) by pulling a rope, start playing sound, play the audio file through the underwater speaker (3), set the playing parameters according to actual conditions, and set the playing time to the required time; screen the large yellow croaker according to the behavioral changes and the area where the large yellow croaker is located after the stimulation ends, screen the large yellow croaker N1 with the most violent reaction and located in the C area farthest from the partition as the intolerant individual, and screen the large yellow croaker N2 with the smallest reaction and located in the A area closest to the partition as the tolerant individual; the screened tolerant individuals and intolerant individuals are transferred to the resting tank (14) for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor (15);

[0019] Step S4, after the remaining 45 large yellow croakers are placed in the noise stress tolerance screening test water tank (4) to recover for a required period of time, they are slowly driven to the temporary rearing area (5) and the partition (6) is lowered. After the remaining 45 large yellow croakers are allowed to recover for a required period of time, the partition is pulled out to carry out a second round of screening;

[0020] Step S5, second round of screening: start the sound playback, the audio file is played through the underwater speaker (3), when the sound playback parameters are set, the frequency remains unchanged, the sound pressure level is increased compared with the first round of screening, and the playback time is set to a preset time; according to the behavioral changes of the yellow croaker and the area where the stimulation is completed, the yellow croaker N3 tails in the C area with the most intense reaction are screened out as intolerant individuals, and the yellow croaker N4 tails in the A area with the smallest reaction are screened out as tolerant individuals; the tolerant individuals and intolerant individuals screened out are transferred to the resting tank (14) for resting, and the status of the yellow croaker is observed and recorded by the underwater temperature and depth monitor (15). Step S6, after the remaining yellow croakers have recovered for the preset time in the noise stress tolerance test tank (4), they are slowly driven to the temporary holding area (5) and the partition (6) is lowered. After the remaining yellow croakers have recovered for the required time, the partition is pulled out and the third round of screening is carried out;

[0021] Step S7, the third round of screening: start the sound playback, the audio file is played through the underwater speaker (3), when the sound playback parameters are set, the frequency remains unchanged, the sound pressure level is increased compared with the second round of screening, and the playback time is set to the required time; according to the behavioral changes of the large yellow croaker and the area where the stimulation is completed, the large yellow croaker N5 with the most intense reaction in area C is screened as an intolerant individual, the large yellow croaker N6 with the smallest reaction in area A is screened as a tolerant individual, and the remaining N7 are moderate individuals. The tolerant individuals, intolerant individuals and moderate individuals screened are all transferred to the resting tank (14) for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth monitor (15);

[0022] Step S8, the acoustic data collected by the hydrophone (10) is processed by Matlab to draw an underwater noise spectrum diagram to ensure the accuracy of the sound release parameters;

[0023] The sound emission parameters in the early stages of the above steps S1 to S8 are calibrated after data collection and analysis by the hydrophone (10).

[0024] When the behavior monitoring system analyzes the state change of the large yellow croaker under noise stimulation, the method evaluates the large yellow croaker by the swimming speed of the large yellow croaker in the camera image of the monitor, calculates the actual movement displacement distance and swimming speed of the large yellow croaker in the device by the behavior analysis of the high-definition camera (9) and the image processing technology, and monitors the state difference of the large yellow croaker in the three static water tanks (14) according to the underwater temperature and depth sensing monitor (15), so as to further verify the accuracy of the screening; specifically,

[0025] The large yellow croaker is moving in the surveillance camera screen, and the actual displacement distance is converted by the pixel vector displacement between two points. The formula is:

[0026]

[0027] Where:

[0028] x i ,y i —ZooTracer pixel coordinates;

[0029] i—starting frame number;

[0030] n—the number of frames used to complete the motion displacement;

[0031] kx, ky—actual distance correction value, kx=1.469, ky=0.380;

[0032] D—actual displacement distance, unit: cm;

[0033] The swimming speed formula is:

[0034]

[0035] Where:

[0036] D—is the actual displacement distance in formula 1;

[0037] fps—the number of frames per second transmitted by the video;

[0038] f i —Number of frames used to complete the vector displacement;

[0039] V—swimming speed, unit: cm·s -1 .

[0040] The advantages of the present invention are:

[0041] The device designed by the present invention is fully functional and can be used as a daily breeding pond when no screening experiment is performed. The overall structure is subjected to noise reduction treatment, and the external stimulation to the cultured fish body is smaller than that in cement breeding ponds, fiberglass breeding ponds, and canvas breeding ponds. The device combines passive acoustic technology with video monitoring technology, and calculates the actual movement displacement distance and swimming speed of the large yellow croaker in the device through behavioral analysis and image processing technology. The device can effectively screen out large yellow croaker individuals that are tolerant and intolerant to noise stress. The large yellow croaker individuals that are screened out to tolerate noise stress can provide basic support for genome-wide association study and transcriptome analysis to screen large yellow croaker auditory sensitivity genes. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The present invention is further described in detail below with reference to the accompanying drawings and specific embodiments:

[0043] Attached Figure 1 It is a schematic diagram of the system framework construction and method flow of the present invention;

[0044] Attached Figure 2 It is a schematic diagram of the overall structure of the device of the present invention;

[0045] Attached Figure 3 is a schematic diagram of underwater noise stimulation data analysis;

[0046] Attached Figure 4 is another schematic diagram of the underwater noise stimulus data analysis;

[0047] In the figure: 1-power amplifier; 2-function generator; 3-underwater speaker; 4-screening noise stress resistance test tank; 5-temporary holding area; 6-partition; 7-behavior screening area; 8-first shockproof and sound-absorbing pad; 9-high-definition camera; 10-hydrophone; 11-letter identification; 12-control water valve; 13-silence pipe; 14-resting tank; 15-underwater temperature and depth monitor; 16-sound-absorbing cotton; 17-second shockproof and sound-absorbing pad; 18-constant temperature tank; 19-temperature sensing control switch; 20-heating rod; 21-circulating water system; 22-filter material; 23-oxygen pump. DETAILED DESCRIPTION

[0048] As shown in the figure, a device for screening large yellow croaker resistant to noise stress based on behavioral response, the screening device includes a breeding system, a behavior monitoring system and a noise stimulation system; the breeding system includes a circulating water device, an aeration pump, a temperature sensing control switch and a heating rod arranged at a water tank; the behavior monitoring system includes a monitor above the water tank and an underwater temperature and depth sensing monitor at the water tank; the noise stimulation system includes a control module, an acoustic stimulation audio synthesis module including a function generator and a power amplifier, an acoustic stimulation signal release module including an underwater speaker, and also includes an underwater acoustic characteristic acquisition and analysis module arranged in different areas of the screening device; the underwater acoustic characteristic acquisition and analysis module includes a hydrophone, a power amplifier and an AD converter; when the screening device is working, the environmental parameters of the water tank where the large yellow croaker is located are first adjusted through the breeding system, and then the large yellow croaker in the water tank is stimulated with noise played directionally by the noise stimulation system, and the state changes of the large yellow croaker under noise stimulation are analyzed by the behavior monitoring system to screen out large yellow croaker resistant to noise stress.

[0049] The power amplifier 1 is connected to the function generator 2 via a data transmission line, and the power amplifier 1 is connected to the underwater speaker 3 and the computer of the control module.

[0050] The water tank includes a static water tank 14 and a screening noise stress resistance test water tank 4, and the underwater speaker is attached to and fixed to the water tank wall of the screening noise stress resistance test water tank 4; the screening noise stress resistance test water tank includes a temporary holding area 5 and a behavior screening area 7 separated by a movable partition 6; the partition can be taken out from the screening noise stress resistance test water tank.

[0051] The underwater speaker is placed on the tank wall of the temporary holding area, and its sound direction is toward the behavior screening area; the bottom of the behavior screening area 7 has area partitions and letter marks 11, and a hydrophone 10 is fixed at the center of each separated area in the behavior screening area 7, and a high-definition camera 9 is fixed directly above the behavior screening area 7. The screening noise stress tolerance experimental tank 4 is connected to the resting tank 14 through a silencer pipe 13, and a control water valve 12 is installed on the silencer pipe 13. When the noise stimulation system starts the noise stress operation, the control water valve 12 is closed.

[0052] A first shockproof and sound-absorbing pad 8 is installed at the bottom of the water tank for screening noise stress resistance experiment; a second shockproof and sound-absorbing pad 17 is installed at the bottom of the static water tank, and sound-absorbing cotton 16 is installed on the side; the first shockproof and sound-absorbing pad and the second shockproof and sound-absorbing pad are made of the same material.

[0053] The static water tank is provided with an underwater temperature and depth monitor 15, which is connected to a constant temperature water tank 18, in which a temperature sensing control switch 19 is fixed, and the temperature sensing control switch 19 is connected to a heating rod 20 for controlling the water temperature of the entire screening device;

[0054] The constant temperature water tank 17 is connected to a circulating water system 21, in which a filter material 22 and an oxygen pump 23 for optimizing water quality are placed to ensure that the water quality and dissolved oxygen content of the entire screening device meet the required standards.

[0055] Each of the large yellow croakers used for screening is provided with a label that can be identified by a behavior monitoring system, so as to distinguish different fish.

[0056] The method for using the screening device specifically comprises the following steps:

[0057] Step S1, screening the temporary rearing area 5 in the noise stress resistance experimental water tank 4 for temporary rearing of experimental fish before the experiment begins, selecting 60 large yellow croakers with healthy and intact body surfaces to be temporarily reared in the temporary rearing area for 3 days, during which the control water valve 12, the constant temperature water tank 18, the temperature sensing control switch 19, the heating rod 20 and the circulating water system 20 are all in an open state to ensure that the device can provide suitable environmental conditions for the large yellow croaker;

[0058] Step S2, before the noise stress begins, the circulating water system 21 and the control water valve 12 are closed to prevent the background noise generated by them from affecting the state of the large yellow croaker; after the circulating water system 21 and the control water valve 12 are closed, the sound release parameters are set according to the auditory characteristics of the large yellow croaker and the stimulation requirements, first ensuring that the power amplifier (1), the function generator 2 and the underwater speaker 3 function normally, then turning on the hydrophone 10 and the high-definition camera 9, and monitoring the underwater noise and video changes in the noise stress resistance screening experiment water tank 4;

[0059] Step S3, first round of screening: quickly and quickly remove the partition 6 by pulling the rope, start the sound playback, the audio file is played through the underwater speaker 3, the sound playback parameters are set to 300Hz frequency, 130dB sound pressure, or set according to actual conditions, and the playback time is set to 5min; screening is performed according to the behavioral changes of the large yellow croaker and the area where it is located after the stimulation ends, and 10 large yellow croakers with the most violent reactions and in the C area farthest from the partition are screened as intolerant individuals, and 5 large yellow croakers with the smallest reactions and in the A area closest to the partition are screened as tolerant individuals; the screened tolerant individuals and intolerant individuals are transferred to the resting tank 14 for rest, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor 15;

[0060] Step S4, after the remaining 45 large yellow croakers are placed in the noise stress tolerance screening test water tank 4 to recover for 15 minutes, they are slowly driven to the temporary rearing area 5 and the partition 6 is lowered, and after the remaining 45 large yellow croakers are allowed to recover for 15 minutes, the partition is pulled out to carry out a second round of screening;

[0061] Step S5, second round of screening: start the sound playback, the audio file is played through the underwater speaker 3, the sound playback parameters are set to 300Hz frequency, 140dB sound pressure, that is, the frequency remains unchanged, the sound pressure level is increased by 10dB compared with the first round of screening, and the playback time is set to 5min; according to the behavioral changes of the large yellow croaker and the area where the stimulation is completed, the 5 large yellow croakers with the most intense reaction in area C are screened as intolerant individuals, and the 5 large yellow croakers with the smallest reaction in area A are screened as tolerant individuals. The screened tolerant individuals and intolerant individuals are transferred to the resting tank 14 for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor 15.

[0062] Step S6, after the remaining 35 large yellow croakers recover in the noise stress tolerance screening test tank 4 for 15 minutes, they are slowly driven to the temporary rearing area 5 and the partition 6 is lowered. After the remaining 35 large yellow croakers recover for 15 minutes, the partition is pulled out and the third round of screening is performed;

[0063] Step S7, the third round of screening: Turn on the sound, the audio file is played through the underwater speaker 3, the sound parameters are set to 300Hz frequency, 150dB sound pressure, that is, the frequency remains unchanged, the sound pressure level is increased by 10dB compared with the second round of screening, and the playing time is set to 5min; according to the behavioral changes of the large yellow croaker and the area where the stimulation is completed, the 5 large yellow croakers with the most intense reaction in area C are screened out as intolerant individuals, the 10 large yellow croakers with the smallest reaction in area A are screened out as tolerant individuals, and the remaining 20 are moderate individuals. The screened tolerant individuals, intolerant individuals, and moderate individuals are all transferred to the resting tank 14 for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth monitor 15;

[0064] Step S8, the acoustic data collected by the hydrophone 10 is processed by Matlab to draw an underwater noise spectrum to ensure the accuracy of the sound emission parameters. The sound emission parameters in the early stages of steps S1 to S8 are also calibrated after analyzing and processing the data collected by the hydrophone 10.

[0065] When the behavior monitoring system analyzes the state change of the large yellow croaker under noise stimulation, the method evaluates the large yellow croaker by the swimming speed of the large yellow croaker in the camera image of the monitor, calculates the actual movement displacement distance and swimming speed of the large yellow croaker in the device by the behavior analysis of the high-definition camera (9) and the image processing technology, and monitors the state difference of the large yellow croaker in the three static water tanks (14) according to the underwater temperature and depth sensing monitor (15), so as to further verify the accuracy of the screening; specifically,

[0066] The large yellow croaker is moving in the surveillance camera screen, and the actual displacement distance is converted by the pixel vector displacement between two points. The formula is:

[0067]

[0068] Where:

[0069] x i ,y i —ZooTracer pixel coordinates;

[0070] i—starting frame number;

[0071] n—the number of frames used to complete the motion displacement;

[0072] kx, ky—actual distance correction value, kx=1.469, ky=0.380;

[0073] D—actual displacement distance, unit: cm;

[0074] The swimming speed formula is:

[0075]

[0076] Where:

[0077] D—is the actual displacement distance in formula 1;

[0078] fps—the number of frames per second transmitted by the video;

[0079] f i —Number of frames used to complete the vector displacement;

[0080] V—swimming speed, unit: cm·s -1 .

[0081] Example:

[0082] In this example, the device includes: a breeding system, a behavior monitoring system and a noise stimulation system. The breeding system is composed of a circulating water device, an aeration pump, a temperature sensing control switch and a heating rod.

[0083] Behavior monitoring system, which consists of a high-definition monitor directly above the tank and an underwater temperature and depth monitor. Noise stimulation system, which is the core part of this device, includes: computer control module, sound stimulation audio synthesis module (function generator, power amplifier), sound stimulation signal release module (underwater speaker), underwater acoustic characteristics acquisition and analysis module in different areas of the device (hydrophone, power amplifier, AD converter). In this example, the device for screening large yellow croaker resistant to noise stress based on behavioral response includes the following three parts: Figure 1 , Figure 2 :

[0084] The breeding system consists of a circulating water device, an aeration pump, a temperature sensing control switch, and a heating rod. The system is mainly used for temporary breeding, noise stress experiments, and resting of large yellow croaker, as well as providing sufficient dissolved oxygen and a constant water temperature for the entire device, ensuring that the water temperature, salinity, dissolved oxygen, etc. are consistent throughout the device to avoid fish stress caused by water quality issues.

[0085] The behavior monitoring system consists of a high-definition monitor directly above the water tank and an underwater temperature and depth monitor. The system is used to monitor the behavioral changes of fish in the temporary, screening and resting areas, including: large yellow croaker floating on the water, large yellow croaker acceleration, large yellow croaker escaping from noise sources, etc. In addition, through video playback, the swimming speed of large yellow croakers of different individuals can be calculated to analyze the state changes of large yellow croakers and better screen large yellow croakers that are resistant to noise stress.

[0086] The noise stimulation system is the core part of the device, including: computer control module, sound stimulation audio synthesis module function generator, power amplifier, sound stimulation signal release module underwater speaker, underwater acoustic characteristics collection and analysis module of different areas in the device hydrophone, power amplifier, AD converter. The sound stimulation audio synthesis module can generate audio files with specific waveforms, specific sound pressure levels and frequencies through function generators and power amplifiers, and can also play files such as ship noise and underwater noise during the operation of offshore wind farms. Specific audio files are played directionally through underwater speakers to stimulate the screening of target individuals of large yellow croaker.

[0087] like Figure 2 As shown: the device includes a power amplifier 1, which is connected to a function generator 2 via a data transmission line, and is also connected to a computer and an underwater speaker 3.

[0088] The screening noise stress resistance experiment water tank 4 is divided into two parts, a temporary holding area 5 and a behavioral screening area 7. The entire bottom of the water tank is equipped with a shockproof and sound-absorbing pad 8. The underwater speaker is attached to and fixed to the water tank wall of the screening noise stress resistance experiment water tank 4. There is a movable partition 6 between the temporary holding area 5 and the behavioral screening area 7, and the partition 6 can be freely taken out.

[0089] There are area dividers and letter logos 11 at the bottom of the behavior screening area 7. A hydrophone 10 is fixed in the center of each area in the behavior screening area 7, and a high-definition camera 9 is fixed directly above the behavior screening area 7. The screening noise stress resistance experimental water tank 4 is connected to the resting water tank 14 through a silencer pipe 13. The silencer pipe 13 is equipped with a control water valve 12. The control water valve 12 needs to be closed when the noise stress begins. The bottom of the resting water tank 14 is equipped with a shockproof and sound-absorbing pad 17 made of the same material as the shockproof and sound-absorbing pad 8, and the side wall of the resting water tank 14 is equipped with a sound-absorbing cotton 16 with a thickness of 5 cm. Each of the resting water tanks 14 is equipped with an underwater temperature and depth monitor 15.

[0090] The static water tank 14 is connected to a constant temperature water tank 18 , in which a temperature sensing control switch 19 is fixed, and the temperature sensing control switch 19 is connected to a heating rod 20 for controlling the water temperature of the entire device.

[0091] The constant temperature water tank 18 is connected to a circulating water system 21, and a filter material 22 and an oxygenation pump 23 are arranged in the circulating water system 21 to ensure that the water quality and dissolved oxygen content of the entire device meet the standards.

[0092] like Figure 1 , Figure 2 , Figure 3 , Figure 4 Shown: A device for screening large yellow croaker resistant to noise stress based on behavioral response, including a breeding system, a behavioral monitoring system and a noise stimulation system.

[0093] The temporary rearing area 5 in the noise stress resistance screening experimental water tank 4 is used for the temporary rearing of experimental fish before the experiment begins. 60 large yellow croakers with healthy and intact body surfaces are selected and temporarily reared in the temporary rearing area for 3 days. During the temporary rearing process, the control water valve 12, the constant temperature water tank 18, the temperature sensing control switch 19, the heating rod 20 and the circulating water system 20 are all in an open state to ensure that the device can provide suitable environmental conditions for the large yellow croaker.

[0094] Before the noise stress begins, the circulating water system 21 and the control water valve 12 need to be closed to prevent the background noise from affecting the state of the large yellow croaker. After the circulating water system 21 and the control water valve 12 are closed, the sound release parameters are set according to the auditory characteristics of the large yellow croaker and the stimulation requirements to ensure that the power amplifier (1), the function generator 2 and the underwater speaker 3 function normally. The hydrophone 10 and the high-definition camera 9 are turned on to monitor the underwater noise and video changes in the noise stress tolerance screening experiment tank 4. The first round of screening: quickly remove the partition 6 by pulling the rope, turn on the sound release, and the audio file is played through the underwater speaker 3. The sound release parameters are set to 300Hz 130dB and can be set according to actual conditions. The playback time is set to 5min. Screening is performed based on the behavioral changes of the large yellow croaker and the area where it is located after the stimulation ends. The 10 large yellow croakers with the most intense reactions in area C are screened as intolerant individuals, and the 5 large yellow croakers with the smallest reactions in area A are screened as tolerant individuals. The screened tolerant individuals and intolerant individuals are transferred to the resting tank 14 for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor 15.

[0095] After the remaining 45 large yellow croakers were allowed to recover in the noise stress tolerance test tank 4 for 15 minutes, they were slowly driven to the temporary rearing area 5 and the partition 6 was lowered. After the remaining 45 large yellow croakers recovered for 15 minutes, a second round of screening was performed.

[0096] Second round of screening: Turn on the sound playback, the audio file is played through the underwater speaker 3, the sound playback parameters are set to 300Hz 140dB frequency unchanged, the sound pressure level is increased by 10dB compared with the first round of screening, and the playback time is set to 5min. Screening is performed based on the behavioral changes of the large yellow croaker and the area where it is located after the stimulation ends. The 5 large yellow croakers with the most intense reactions in area C are screened as intolerant individuals, and the 5 large yellow croakers with the smallest reactions in area A are screened as tolerant individuals. The screened tolerant individuals and intolerant individuals are transferred to the resting tank 14 for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor 15.

[0097] After the remaining 35 large yellow croakers were recovered in the noise stress tolerance test tank 4 for 15 minutes, they were slowly driven to the temporary holding area 5 and the partition 6 was lowered. After the remaining 35 large yellow croakers recovered for 15 minutes, a third round of screening was performed.

[0098] The third round of screening: Turn on the sound, the audio file is played through the underwater speaker 3, the sound parameters are set to 300Hz 150dB frequency unchanged, the sound pressure level is increased by 10dB compared with the second round of screening, and the playing time is set to 5min. Screening is performed based on the behavioral changes of the large yellow croaker and the area where it is located after the stimulation ends. The 5 large yellow croakers with the most intense reaction in area C are screened as intolerant individuals, the 10 large yellow croakers with the smallest reaction in area A are screened as tolerant individuals, and the remaining 20 are moderate individuals. The screened tolerant individuals, intolerant individuals, and moderate individuals are all transferred to the resting tank 14 for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth monitor 15.

[0099] The acoustic data collected by the hydrophone 10 is processed by Matlab to draw an underwater noise spectrum diagram to ensure the accuracy of the sound release parameters. The early sound release parameters are also calibrated after the data collected by the hydrophone 10 is analyzed and processed. The actual movement displacement distance and swimming speed of the large yellow croaker in the device are calculated by the behavioral analysis and image processing technology of the high-definition camera 9, and the state differences of the large yellow croaker in the three static water tanks 14 are monitored by the underwater temperature and depth monitor 15 to further verify the accuracy of the screening.

[0100] The above descriptions are only some embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Any equivalent device or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A device for screening large yellow croaker resistant to noise stress based on behavioral response, characterized in that: The screening device comprises a breeding system, a behavior monitoring system and a noise stimulation system; the breeding system comprises a circulating water device, an oxygenation pump, a temperature sensing control switch and a heating rod arranged at a water tank; the behavior monitoring system comprises a monitor above the water tank and an underwater temperature and depth sensing monitor at the water tank; the noise stimulation system comprises a control module, an acoustic stimulation audio synthesis module comprising a function generator and a power amplifier, an acoustic stimulation signal release module comprising an underwater speaker, and also comprises an underwater acoustic characteristic acquisition and analysis module arranged at different areas of the screening device; the underwater acoustic characteristic acquisition and analysis module comprises a hydrophone, a power amplifier and an AD converter; when the screening device is working, the environmental parameters of the water tank where the large yellow croaker is located are first adjusted through the breeding system, and then the large yellow croaker in the water tank is stimulated with noise played directionally by the noise stimulation system, and the state changes of the large yellow croaker under noise stimulation are analyzed by the behavior monitoring system to screen out large yellow croaker resistant to noise stress.

2. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 1, characterized in that: The power amplifier (1) is connected to the function generator (2) via a data transmission line, and the power amplifier (1) is connected to the underwater speaker (3) and the computer of the control module.

3. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 2, characterized in that: The water tank comprises a resting water tank (14) and a screening noise stress resistance test water tank (4); the underwater speaker is attached to and fixed to the water tank wall of the screening noise stress resistance test water tank (4); the screening noise stress resistance test water tank comprises a temporary holding area (5) and a behavior screening area (7) separated by a movable partition (6); the partition can be removed from the screening noise stress resistance test water tank.

4. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 3, characterized in that: The underwater speaker is placed on the wall of the tank in the temporary breeding area, and its sound emission direction is toward the behavior screening area; the bottom of the behavior screening area (7) has area partitions and letter marks (11), a hydrophone (10) is fixed at the center of each separated area in the behavior screening area (7), and a high-definition camera (9) is fixed directly above the behavior screening area (7).

5. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 3, characterized in that: The noise stress resistance screening test water tank (4) is connected to the static water tank (14) via a silencing pipe (13). A control water valve (12) is installed on the silencing pipe (13). When the noise stimulation system starts the noise stress operation, the control water valve (12) is closed.

6. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 3, characterized in that: A first shockproof and sound-absorbing pad (8) is installed at the bottom of the water tank for screening noise stress resistance experiment; a second shockproof and sound-absorbing pad (17) is installed at the bottom of the resting water tank, and sound-absorbing cotton (16) is installed on the side; the first shockproof and sound-absorbing pad and the second shockproof and sound-absorbing pad are made of the same material.

7. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 3, characterized in that: An underwater temperature and depth sensing monitor (15) is provided in the static water tank, and the static water tank is connected to a constant temperature water tank (18). A temperature sensing control switch (19) is fixed in the constant temperature water tank (18), and the temperature sensing control switch (19) is connected to a heating rod (20) for controlling the water temperature of the entire screening device; The constant temperature water tank (17) is connected to a circulating water system (21), and a filter material (22) and an oxygen pump (23) for optimizing water quality are placed in the circulating water system (21), so as to ensure that the water quality and dissolved oxygen content of the entire screening device meet the required standards.

8. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 7, characterized in that: Each of the large yellow croakers used for screening is provided with a label that can be identified by a behavior monitoring system, so as to distinguish different fish.

9. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 7, characterized in that: The method for using the screening device specifically comprises the following steps: Step S1, screening the temporary rearing area (5) in the noise stress resistance experimental water tank (4) for temporary rearing of experimental fish before the experiment begins, selecting 60 large yellow croakers with healthy and intact body surfaces to be temporarily reared in the temporary rearing area for 3 days, during which the control water valve (12), the constant temperature water tank (18), the temperature sensing control switch (19), the heating rod (20) and the circulating water system (20) are all in an open state to ensure that the device can provide suitable environmental conditions for the large yellow croakers; Step S2, before the noise stress begins, the circulating water system (21) and the control water valve (12) are closed to prevent the background noise generated by them from affecting the state of the large yellow croaker; after the circulating water system (21) and the control water valve (12) are closed, the sound release parameters are set according to the auditory characteristics of the large yellow croaker and the stimulation requirements, first ensuring that the power amplifier (1), the function generator (2) and the underwater speaker (3) function normally, then turning on the hydrophone (10) and the high-definition camera (9), and monitoring the underwater noise and video changes in the noise stress resistance screening test tank (4); Step S3, first round of screening: quickly and rapidly remove the partition (6) by pulling a rope, start playing sound, play the audio file through the underwater speaker (3), set the playing parameters according to actual conditions, and set the playing time to the required time; screen the large yellow croaker according to the behavioral changes and the area where the large yellow croaker is located after the stimulation ends, screen the large yellow croaker N1 with the most violent reaction and located in the C area farthest from the partition as the intolerant individual, and screen the large yellow croaker N2 with the smallest reaction and located in the A area closest to the partition as the tolerant individual; the screened tolerant individuals and intolerant individuals are transferred to the resting tank (14) for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor (15); Step S4, after the remaining 45 large yellow croakers are placed in the noise stress tolerance screening test water tank (4) to recover for a required period of time, they are slowly driven to the temporary rearing area (5) and the partition (6) is lowered. After the remaining 45 large yellow croakers are allowed to recover for a required period of time, the partition is pulled out to carry out a second round of screening; Step S5, second round of screening: start playing sound, the audio file is played through the underwater speaker (3), when the playing parameters are set, the frequency remains unchanged, the sound pressure level is increased compared with the first round of screening, and the playing time is set to a preset time; screening is performed according to the behavioral changes of the yellow croaker and the area where the yellow croaker is located after the stimulation ends, and the yellow croaker N3 with the most intense reaction in area C is screened out as an intolerant individual, and the yellow croaker N4 with the smallest reaction in area A is screened out as a tolerant individual; the screened tolerant individuals and intolerant individuals are transferred to the resting tank (14) for resting, and the status of the yellow croaker is observed and recorded by the underwater temperature and depth sensing monitor (15). Step S6, after the remaining large yellow croakers have recovered in the noise stress tolerance test water tank (4) for a preset time, they are slowly driven to the temporary rearing area (5) and the partition (6) is lowered; after the remaining large yellow croakers have recovered for a required time, the partition is pulled out and a third round of screening is performed; Step S7, the third round of screening: start the sound playback, the audio file is played through the underwater speaker (3), when the sound playback parameters are set, the frequency remains unchanged, the sound pressure level is increased compared with the second round of screening, and the playback time is set to the required time; according to the behavioral changes of the large yellow croaker and the area where the stimulation is completed, the large yellow croaker N5 with the most intense reaction in area C is screened as an intolerant individual, the large yellow croaker N6 with the smallest reaction in area A is screened as a tolerant individual, and the remaining N7 are moderate individuals. The tolerant individuals, intolerant individuals and moderate individuals screened are all transferred to the resting tank (14) for resting, and the status of the large yellow croaker is observed and recorded by the underwater temperature and depth monitor (15); Step S8, the acoustic data collected by the hydrophone (10) is processed by Matlab to draw an underwater noise spectrum diagram to ensure the accuracy of the sound release parameters; The sound emission parameters in the early stages of the above steps S1 to S8 are calibrated after data collection and analysis by the hydrophone (10).

10. The device for screening large yellow croaker resistant to noise stress based on behavioral response according to claim 9, characterized in that: When the behavior monitoring system analyzes the state change of the large yellow croaker under noise stimulation, the method evaluates the large yellow croaker by the swimming speed of the large yellow croaker in the camera image of the monitor, calculates the actual movement displacement distance and swimming speed of the large yellow croaker in the device by the behavior analysis of the high-definition camera (9) and the image processing technology, and monitors the state difference of the large yellow croaker in the three static water tanks (14) according to the underwater temperature and depth sensing monitor (15), so as to further verify the accuracy of the screening; specifically, The large yellow croaker is moving in the surveillance camera screen, and the actual displacement distance is converted by the pixel vector displacement between two points. The formula is: Where: x i ,y i —ZooTracer pixel coordinates; i—starting frame number; n—the number of frames used to complete the motion displacement; kx, ky—actual distance correction value, kx=1.469, ky=0.380; D—actual displacement distance, unit: cm; The swimming speed formula is: Where: D—is the actual displacement distance in formula 1; fps—the number of frames per second transmitted by the video; f i —Number of frames used to complete the vector displacement; V—swimming speed, unit: cm·s -1 .

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