Traditional Chinese medicine mouthwash with bacteriostatic effect and preparation method thereof

Abstract

The invention relates to the technical field of mouthwash, in particular to traditional Chinese medicine mouthwash with an antibacterial effect and a preparation method of the traditional Chinese medicine mouthwash. According to the preparation method of the traditional Chinese medicine mouthwash with the bacteriostatic effect, honeysuckle, dandelion, lalang grass rhizome, purslane, chrysanthemum, mint and liquorice are used as a formula, and the traditional Chinese medicine mouthwash is prepared by the following steps: preparing materials according to the monarch, minister, assistant and guide formula of traditional Chinese medicine, decocting, concentrating, cooling, filtering, mixing with saline water to prepare a 0.9% NaCl solution, packaging, sterilizing and processing. Wherein the monarch drug is prepared from 10 to 15 parts of honeysuckle flower and 10 to 15 parts of herba taraxaci; ministerial drugs: 10-15 parts of lalang grass rhizome and 10-15 parts of purslane; adjuvant drugs: 5-10 parts of chrysanthemum and 5-10 parts of mint; and a conductant drug: 5-10 parts of licorice. The mouthwash is prepared from natural medicinal and edible traditional Chinese medicines, the compatibility of the whole formula is reasonable, the components are mutually synergistic, and the number of pathogenic microorganisms in the oral cavity can be remarkably reduced, so that the morbidity of oral ulcer and angular cheilitis caused by staphylococcus aureus, escherichia coli and the like is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of mouthwash, in particular to a traditional Chinese medicine mouthwash with antibacterial effect and a preparation method thereof. Background Art

[0002] At present, the commonly used mouthwashes in clinical practice contain antibiotics, iodine, ethanol and other ingredients, such as chlorhexidine and new iodine-containing mouthwashes, which can effectively reduce bacteria in dental plaque, reduce the toxicity of bacterial products, control plaque, and prevent oral diseases such as gingivitis and periodontitis. However, long-term use of mouthwashes containing broad-spectrum antibiotics can easily cause oral flora imbalance. With the increase in the number of uses, the risk of drug-resistant bacteria and secondary infection is also higher. Chlorhexidine mouthwashes can easily cause tooth staining and taste changes after long-term use. They are not suitable for long-term use. They are not accepted by patients because of their bitter taste, poor taste, and large local irritation. Some patients may even have allergies and cause rashes or stomatitis. The effective iodine content of iodine-containing mouthwashes is unstable, and the effective content decreases rapidly after dilution. They need to be prepared on the spot, which brings inconvenience to patients. Therefore, finding a natural, effective, safe, alcohol-free, long-term use mouthwash has become a new hot spot for treating oral problems. Summary of the invention

[0003] The purpose of the present invention is to provide a Chinese medicinal mouthwash with antibacterial effect and a preparation method thereof in view of the above-mentioned deficiencies in the prior art.

[0004] The invention discloses a method for preparing a Chinese medicine mouthwash with antibacterial effect, which comprises taking honeysuckle, dandelion, Imperata root, purslane, chrysanthemum, mint and liquorice as a formula, preparing the ingredients according to the formula of monarch, minister, assistant and envoy of traditional Chinese medicine, decocting, concentrating, cooling, filtering, mixing with salt water to prepare 0.9% NaCl solution, encapsulating and sterilizing the solution;

[0005] Among them, the main medicine: honeysuckle 10-15 parts, dandelion 10-15 parts

[0006] Ministerial drugs: 10-15 parts of Imperata root and 10-15 parts of Portulaca oleracea

[0007] Adjuvant: 5-10 parts of chrysanthemum, 5-10 parts of mint

[0008] Assistant drug: 5-10 parts of licorice.

[0009] Furthermore, 5-9 times the volume of water is added to the medicinal material powder, and the mixture is decocted and extracted for 2-4 times, and the extracts are combined and concentrated to a concentrated solution with a relative density of 1-2 g crude drug / ml.

[0010] Furthermore, the concentrated solution is cooled and filtered and then mixed with saline to prepare a 0.9% NaCl mouthwash.

[0011] Furthermore, the mass concentration of the mouthwash is 1-2 g / 10 mL of crude drug.

[0012] Further, add water and boil for 3 times.

[0013] Furthermore, the first time is 9 times the volume of water, the second time is 6 times the volume of water, and the third time is 5 times the volume of water.

[0014] Further, the main medicine: 10 parts of honeysuckle and 10 parts of dandelion

[0015] Ministerial drugs: 10 parts of Imperata Root and 10 parts of Portulaca Oleracea

[0016] Adjuvant: 5 parts chrysanthemum, 5 parts mint

[0017] Assistant drug: 5 parts of licorice.

[0018] A Chinese medicinal mouthwash with antibacterial effect prepared by the above-mentioned preparation method.

[0019] The invention uses natural Chinese medicine and edible dual-purpose medicine to prepare the mouthwash. The whole formula is reasonably compatible and the components cooperate with each other. The number of oral pathogenic microorganisms, including Escherichia coli and Staphylococcus aureus, can be significantly reduced, thereby reducing the incidence of oral ulcers and angular cheilitis caused by Staphylococcus aureus, Escherichia coli, etc.

[0020] The invention has the effects of cleaning the oral cavity, inhibiting gum bleeding, clearing away heat and detoxifying. In addition, the traditional Chinese medicine compound preparation process of the invention is simple, which is conducive to the realization of industrialization, and is applicable to a wide range of groups, and can be used by pregnant women and children without adverse reactions. DETAILED DESCRIPTION

[0021] The following are specific embodiments of the present invention, which further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0022] Example 1

[0023] The invention discloses a method for preparing a Chinese medicine mouthwash with antibacterial effect, which comprises taking honeysuckle, dandelion, Imperata root, purslane, chrysanthemum, mint and liquorice as a formula, preparing the ingredients according to the formula of monarch, minister, assistant and envoy of traditional Chinese medicine, decocting, concentrating, cooling, filtering, mixing with salt water to prepare 0.9% NaCl solution, encapsulating and sterilizing the solution;

[0024] Among them, the main medicine: 10 parts of honeysuckle and 10 parts of dandelion

[0025] Ministerial drugs: 10 parts of Imperata Root and 10 parts of Portulaca Oleracea

[0026] Adjuvant: 5 parts chrysanthemum, 5 parts mint

[0027] Assistant drug: 5 parts of licorice.

[0028] Example 2

[0029] Main medicine: 10 parts of honeysuckle and 15 parts of dandelion

[0030] Ministerial drugs: 15 parts of Imperata Root, 10 parts of Portulaca Oleracea

[0031] Adjuvant: 5 parts of chrysanthemum, 10 parts of mint

[0032] Assistant drug: 5 parts of licorice.

[0033] Example 3

[0034] Main medicine: 15 parts of honeysuckle and 10 parts of dandelion

[0035] Ministerial drugs: 10 parts of Imperata Root and 15 parts of Portulaca Oleracea

[0036] Adjuvant: 10 parts of chrysanthemum, 7 parts of mint

[0037] Assistant drug: 10 parts of licorice.

[0038] Example 4

[0039] Main medicine: 12 parts of honeysuckle and 13 parts of dandelion

[0040] Ministerial drugs: 12 parts of Imperata Root and 13 parts of Portulaca Oleracea

[0041] Adjuvant: 7 parts chrysanthemum, 8 parts mint

[0042] Assistant drug: 6 parts of licorice.

[0043] Comparative Example 1

[0044] Main medicine: 20 parts of honeysuckle

[0045] Ministerial drugs: 10 parts of Imperata Root and 10 parts of Portulaca Oleracea

[0046] Adjuvant: 5 parts chrysanthemum, 5 parts mint

[0047] Assistant drug: 5 parts of licorice.

[0048] Comparative Example 2

[0049] Main medicine: 20 parts of dandelion

[0050] Ministerial drugs: 10 parts of Imperata Root and 10 parts of Portulaca Oleracea

[0051] Adjuvant: 5 parts chrysanthemum, 5 parts mint

[0052] Assistant drug: 5 parts of licorice.

[0053] Comparative Example 3

[0054] Main medicine: 10 parts of honeysuckle and 10 parts of dandelion

[0055] Assistant medicine: 2 parts of Imperata root, 10 parts of Portulaca oleracea

[0056] Adjuvant: 5 parts chrysanthemum, 5 parts mint

[0057] Assistant drug: 5 parts of licorice.

[0058] The above examples and comparative examples were subjected to antibacterial assay.

[0059] The inhibition zone was determined by the disc diffusion method:

[0060] Evenly spread 120uL of the 1000-fold diluted McFarland concentration Staphylococcus aureus solution or Escherichia coli solution on the prepared agar plate and let it stand until the solution is absorbed.

[0061] Place 10 filter paper sheets in a glass plate; shake the sample vigorously, quickly take a sample of 10 μL and drop it onto the filter paper, then use sterile tweezers to stick the filter paper sheet on the agar surface coated with the bacterial solution, gently press the paper sheet to make it properly contact with the agar, stick three paper sheets on each plate, parallel three plates, and make the spacing between each paper sheet equal. Then observe the antibacterial zone after incubating in a 37°C incubator for 24 hours, and then use a vernier caliper to measure the diameter of each antibacterial zone, and take the average value and count. The antibacterial results of the embodiment and the comparative example are shown in Table 1.

[0062] Table 1

[0063]

[0064] It can be seen from the above table that the Chinese herbal antibacterial mouthwash of the present invention has a reasonable formula and has good antibacterial effects on Staphylococcus aureus and Escherichia coli.

[0065] 50 groups of passers-by were used to evaluate the sensory and odor properties of the product.

[0066] The test results of sensory and odor are shown in Table 2:

[0067] Table 2

[0068]

[0069]

[0070] Long-term use effect test

[0071] A total of 210 patients with oral problems such as oral ulcers and bleeding gums were selected, half of them were male and half were female, with an average age of 35 years old. They were randomly divided into 7 groups, with 30 people in each group.

[0072] The patient used equal amounts (20 ml) of the Chinese medicine mouthwash prepared in Example 14 and Comparative Example 13, metronidazole gargle, and distilled water, gargled for 3 minutes before gargling. Gargled three times a day after breakfast, lunch, and dinner for 2 consecutive weeks.

[0073] Efficacy criteria: Significant improvement: Oral problems such as oral ulcers, bleeding gums, and bad breath are significantly improved or disappear.

[0074] Improvement: Oral problems have been improved to some extent, but not significantly.

[0075] Ineffective: Oral problems were not improved. Worsened: Oral problems worsened. The results are shown in Table 3:

[0076] Table 3

[0077]

[0078]

[0079] It can be seen from the above table that the Chinese medicine antibacterial mouthwash of the present invention has a reasonable compatibility, and the components can act synergistically, which is better than using some components alone, and its therapeutic effect is also more significant.

[0080] For matters not mentioned above, the prior art applies.

[0081] Although some specific embodiments of the present invention have been described in detail through examples, those skilled in the art should understand that the above examples are for illustration only and are not intended to limit the scope of the present invention. Those skilled in the art to which the present invention belongs may make various modifications or supplements to the specific embodiments described or replace them in a similar manner, but they will not deviate from the direction of the present invention or exceed the scope defined by the attached claims. Those skilled in the art should understand that any modifications, equivalent substitutions, improvements, etc. made to the above embodiments based on the technical essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a Chinese medicinal mouthwash with antibacterial effect, characterized in that: The composition is honeysuckle, dandelion, Imperata root, purslane, chrysanthemum, mint and liquorice, and the ingredients are prepared according to the TCM formula of monarch, minister, assistant and envoy, boiled, concentrated, cooled, filtered, mixed with brine to prepare 0.9% NaCl solution for packaging, and sterilized. Among them, the main medicine: honeysuckle 10-15 parts, dandelion 10-15 parts Ministerial drugs: 10-15 parts of Imperata root and 10-15 parts of Portulaca oleracea Adjuvant: 5-10 parts of chrysanthemum, 5-10 parts of mint Assistant drug: 5-10 parts of licorice.

2. The preparation method according to claim 1, characterized in that: Add 5-9 times volume of water to the medicinal material powder, decoct and extract for 2-4 times, combine the extracts, and concentrate to a concentrated solution with a relative density of 1-2 g crude drug / ml.

3. The preparation method according to claim 2, characterized in that: The concentrate was cooled and filtered and then mixed with saline to prepare a 0.9% NaCl mouthwash.

4. The preparation method according to claim 3, characterized in that: The mass concentration of the mouthwash is 1-2 g / 10 mL of crude drug.

5. The preparation method according to claim 2, characterized in that: Add water and boil for 3 times.

6. The preparation method according to claim 5, characterized in that: The first time is 9 times the volume of water, the second time is 6 times the volume of water, and the third time is 5 times the volume of water.

7. The preparation method according to claim 2, characterized in that: Main medicine: 10 parts of honeysuckle and 10 parts of dandelion Ministerial drugs: 10 parts of Imperata Root and 10 parts of Portulaca Oleracea Adjuvant: 5 parts chrysanthemum, 5 parts mint Assistant drug: 5 parts of licorice.

8. A Chinese medicinal mouthwash with antibacterial effect prepared by the preparation method according to any one of claims 1 to 7.

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