Method for preparing sterile compound multi-sticky product and integrated production equipment thereof

Abstract

The invention belongs to the field of sterile products, and relates to a laboratory sterile ointment preparation technology, in particular to a method for preparing a sterile compound multi-sticky product and integrated production equipment thereof.The method specifically comprises the steps that A, white vaseline and yellow vaseline are preheated and melted to obtain flowing type white vaseline and flowing type yellow vaseline; b, respectively putting white vaseline, yellow vaseline and light liquid paraffin into the three beakers A for sterilization treatment; cooling the white vaseline and the yellow vaseline in the beaker B, adding the white vaseline and the yellow vaseline into the standby main medicine suspension, and mixing and stirring the white vaseline and the yellow vaseline to obtain a sterile compound multi-sticky product; the sterile compound multi-sticky product prepared by the method is simple and effective, and multiple groups of experiments are performed on the sterile compound multi-sticky product by adopting integrated equipment, so that the whole design is more efficient, and the labor is correspondingly saved.

Description

Technical Field

[0001] The present invention belongs to the field of sterile products, relates to laboratory preparation technology of sterile ointment, and in particular to a method for preparing a sterile compound multi-adhesive product and an integrated production equipment thereof. Background Art

[0002] Human skin is an important physical and chemical barrier against pathogen invasion. When the integrity of this barrier is compromised, infection may occur. Studies have shown that the infection rate of small wounds in daily life ranges from 2.5% to 47%. If left untreated, wound infections can develop into more serious soft tissue infections such as cellulitis, pyoderma and necrotizing fasciitis.

[0003] Compound polymyxin B ointment is a compound preparation composed of polymyxin B sulfate, neomycin sulfate, bacitracin and lidocaine hydrochloride. It is a safe and highly effective topical antibacterial drug used to prevent bacterial infections caused by minor cuts, abrasions and burns. Similar products of this compound preparation abroad are called Triple Antibiotic Ointment (TAO).

[0004] One of the active ingredients of this product is neomycin sulfate, an aminoglycoside antibiotic that can bind to the 30S subunit of the bacterial ribosome and interfere with the transcription of mRNA, thereby inhibiting the biosynthesis of bacterial proteins. Neomycin has good inhibitory activity against Gram-negative bacteria and a variety of staphylococci. In vitro tests have shown that the minimum inhibitory concentration of neomycin against Staphylococcus aureus ranges from 0.4 to 1.6 μg / ml. Due to the existence of neomycin-resistant Staphylococcus aureus, the use of neomycin alone to treat bacterial infections is insufficient. However, the incidence of neomycin resistance has not changed in the past 50 years. Anderson reported in 1976 that no increase in neomycin resistance events has been found in in vitro tests since 1956. So far, there have been no reports of increased neomycin resistance since 1980.

[0005] Bacitracin is another active ingredient of this product and belongs to the class of polypeptide antibiotics. The antibacterial effect of bacitracin is achieved by inhibiting the dephosphorylation reaction of C55 isoprenyl pyrophosphate, a diphosphate transport molecule essential for the synthesis of bacterial cell wall peptidoglycan. Bacitracin has a good inhibitory effect on Staphylococcus. Although the inhibitory effect of bacitracin alone on Staphylococcus aureus in in vitro tests is not particularly strong, with a minimum inhibitory concentration of 256 μg / ml, the combined use of neomycin and bacitracin can produce a synergistic antibacterial effect. This is because bacitracin destroys the integrity of the bacterial cell wall, which is very beneficial for increasing the effective concentration of neomycin in bacterial cells. In addition, due to the different antibacterial mechanisms and the existence of synergy, bacterial resistance to neomycin is no longer a problem when using a neomycin-bacitracin combination preparation. Bacitracin is also very effective against Streptococcus pyogenes. Streptococcus pyogenes is the second most common pathogen causing external wound infection. Anderson[5] reported that bacitracin can achieve a 67%-75% antibacterial effect at a concentration of 0.8-6.3 μg / ml. Rodgers et al.'s study in 1997 showed that the MIC90 of bacitracin against 9 strains of test bacteria was 0.5 μg / ml. At the same time, all studies have shown that the sensitivity of Streptococcus pyogenes to bacitracin has not changed in the past 25 years.

[0006] The third active ingredient contained in this product is polymyxin B sulfate. Polymyxin B is also a polypeptide antibiotic. Its antibacterial effect is achieved by increasing the permeability of the bacterial cell wall, destroying the osmotic pressure balance of the cell wall, and causing cell lysis. Pseudomonas aeruginosa, one of the three most common pathogens of skin infection, is highly sensitive to polymyxin. Early studies have found that polymyxin can kill 88% of the tested Pseudomonas aeruginosa at a concentration of 0.01uM / ml. In 1993, Deguehi measured the MIC90 of polymyxin against 50 strains of Pseudomonas aeruginosa to be 6.25μg / ml. Rahal reported in a recent review that polymyxin B, especially when used in combination with β-lactam antibiotics, can play an antibacterial role against Pseudomonas aeruginosa or resistant Acinetobacter that are resistant to existing antibiotics except polymyxin. The article points out that the combined use of antibiotics can increase the antibacterial activity of antibiotics through synergistic effects and reduce the toxicity and incidence of single component resistance. Therefore, although bacitracin itself has no activity against Pseudomonas aeruginosa, it enhances the antibacterial activity of polymyxin B. Earlier studies have confirmed that the combined use of polymyxin B and bacitracin has a synergistic effect in killing bacteria. In the past 50 years, polymyxins have not been used much in clinical practice, so resistance to multidrug-resistant bacteria is still rare. In recent years, reports of polymyxin resistance have gradually increased. Fortunately, Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae, the three "super crazy bacteria" highlighted by the Infectious Diseases Society of America, do not have a high resistance rate to polymyxins.

[0007] Lidocaine hydrochloride is the fourth active ingredient of this product. It not only achieves the effect of local anesthesia, analgesia and itching in the cortex by accumulating in the cortical pain receptors and nerve endings, but also achieves anti-inflammatory effect by reducing the synthesis and release of inflammatory mediators and interfering with the inflammatory cascade reaction.

[0008] Currently, such products on the market and products in the research and development stage are all used for wound treatment. According to the pharmacopoeia, they should be sterile preparations, but all those on the market are non-sterile products, which increases the risk of wound reinfection. Therefore, the present invention prepares a sterile product with multiple antimicrobial spectra for clinical use to benefit patients. Summary of the invention

[0009] In view of the above problems, the present invention provides a method for preparing a sterile compound multi-adhesive product and an integrated production device thereof.

[0010] In order to achieve the above object, the technical solution adopted by the present invention is:

[0011] A method for preparing a sterile compound multi-adhesive product, characterized in that the sterile compound multi-adhesive product specifically comprises the following components:

[0012] Components Number of copies White Vaseline 50 servings Yellow Vaseline 45 servings Light liquid paraffin 20 servings Sterile Material A 5 servings Sterile Material B 4 servings

[0013] The specific preparation method is as follows:

[0014] A. Preheat and melt white vaseline and yellow vaseline respectively to obtain flowable white vaseline and yellow vaseline;

[0015] B. Then, put white vaseline, yellow vaseline and light liquid paraffin into three beakers A respectively for sterilization;

[0016] C. Add white vaseline and yellow vaseline into two beakers B respectively and heat them in a water bath to keep them warm;

[0017] D. Place light liquid paraffin in beaker B, add sterile raw material A into beaker B, and stir to mix to obtain homogenized liquid A;

[0018] E. Add the weighed sterile material B to the homogenized solution, and mix and stir the standby main drug suspension;

[0019] F. Cool the white vaseline and yellow vaseline in beaker B, add them to the standby main drug suspension, and mix and stir them to obtain a sterile compound multi-viscosity product.

[0020] Preferably, the sterilization temperature in step B is 145-160° C. and the duration is 1-3 h.

[0021] Preferably, the water bath heating temperature in step C is 80-90°C.

[0022] Preferably, the sterile material A is any one of lidocaine hydrochloride, pramoxine hydrochloride, procaine hydrochloride, tetracaine hydrochloride, prilocaine hydrochloride, and ropivacaine hydrochloride.

[0023] Preferably, the sterile material B is an antibiotic, such as polymyxin B sulfate, neomycin sulfate and bacitracin (bacitracin zinc). It is best to also talk about the general concept here.

[0024] Preferably, the cooling temperature in step F is to 45-50° C., and the temperature is kept constant for 8-12 minutes after cooling.

[0025] Preferably, the mixing and stirring time in step F is 40-50 min, and the rotation speed is 1500 r / min.

[0026] An integrated device for preparing a sterile compound multi-viscous product, an integrated device for preparing a sterile compound multi-viscous product in any one of claims 1 to 6, characterized in that it comprises a main workbench, a new type of laminar flow hood is arranged above the main workbench, the main workbench and the new laminar flow hood are connected and fixed by a fixed square tube, a sterilization component, a preheating component, and a mixing and stirring component are arranged from top to bottom between the main workbench and the new laminar flow hood, three storage tanks are arranged on both sides of the new laminar flow hood, a supporting fixing rod is arranged on the fixed square tube, and a first new stirring mechanism and a second new stirring mechanism are arranged on the supporting fixing rod.

[0027] The sterilization component, preheating component and mixing component are fixed on the main workbench through connecting rods.

[0028] The sterilization component comprises a rotating disc, on which three new beakers A are arranged. The bottom of the new beaker A is a conical structure. A heating jacket is arranged on the outside of the new beaker A, and a small vibrating rod is arranged on the bottom.

[0029] The preheating component includes a preheating part and a cooling part.

[0030] The preheating part includes the outer wall of the beaker, the inner wall of the beaker, the auxiliary cup wall, a closed cooling pipe, a circulating cooling pipe, a circulating pump connection port, a water pipe connection port, and a cup wall sliding sleeve. The inner side of the outer wall of the beaker is connected to the inner wall of the beaker, and the lower part of the outer wall of the beaker is connected to multiple closed cooling pipes. There is cold water inside the closed cooling pipe, and the inner wall of the beaker is connected to the circulating cooling pipe. There is circulating cold water inside the circulating cooling pipe. The left side of the circulating cooling pipe has a circulating pump connection port, and the left side of the circulating pump connection port passes through the outer wall of the beaker to connect the circulating pump. The right side of the circulating cooling pipe has a water pipe connection port, and the water pipe connection port passes through the outer wall of the beaker to connect the water pipe. The outer side of the inner wall of the beaker has a cup wall sliding sleeve, and the outer side of the cup wall sliding sleeve is connected to the circulating cooling pipe. The inner side of the cup wall sliding sleeve is connected to the auxiliary cup wall through a ring screw, and the auxiliary cup wall slides in the cup wall sliding sleeve, and the auxiliary cup wall has fastening grooves on both sides.

[0031] The cooling part includes a cooling base, a cooling fan is arranged on the cooling base, a rotating motor for driving the cooling fan to rotate is arranged in the cooling base, a manual switch for controlling the start and stop of the rotating motor is arranged on the outer wall of the cooling base, baffles are arranged around the top of the cooling base, the baffles form a rectangular frame, a cooling inner frame is in the rectangular frame, the cooling inner frame is a mesh structure, a placement outer edge extending outward is arranged on the top of the cooling inner frame; a plurality of partitions are arranged in the cooling inner frame; the cooling inner frame is made of plastic material.

[0032] The mixing and stirring assembly includes a base and a frame, a central motor is installed in the middle of the base, and the shaft end of the central motor is connected to the central rotating shaft, a support is fixed to the upper end of the central rotating shaft, and a cushion ball is arranged between the support and the base, unit motors are installed on the left and right sides of the support, and the shaft end of the unit motor is connected to the unit rotating shaft, and heat dissipation nets are arranged on the left and right sides of the unit motor, stirring motors are arranged on the left and right sides above the frame, and the bottom end of the frame is welded to the upper surface of the base, the shaft end of the stirring motor is connected to the stirring shaft, and a ring is arranged on the lower outer side of the stirring shaft.

[0033] The support platform forms a rotating structure with the base through a central rotating shaft and a central motor, and the unit motors are symmetrical about the symmetry center line of the support platform. A chuck is fixed to the upper end of the unit rotating shaft, and a hydraulic cylinder is arranged at the inner bottom of the chuck. A hydraulic oil pipe is connected to a port on the side of the hydraulic cylinder away from the vertical center line of the chuck.

[0034] The inner wall of the chuck is evenly and evenly provided with hydraulic telescopic rods in a ring shape, and the hydraulic telescopic rods are all connected with the hydraulic oil pipes, and a rubber pad is bonded to the side of the hydraulic telescopic rod close to the vertical center line of the chuck, and a cup holder is fixed on the upper inner side of the chuck, and a protrusion is provided in the middle of the upper surface of the cup holder, the protrusion is a truncated cone structure, and anti-slip pads are evenly provided around the protrusion.

[0035] A limiting pressure ring is fixed on the upper surface of the support, and the inner side of the limiting pressure ring fits with the edge of the upper surface of the chuck. A fixing rod is fixed on the upper surface of the limiting pressure ring, and two fixing rods are symmetrically arranged about the vertical center line of the chuck.

[0036] A pressure edge frame is installed above the fixing rod, and the fixing rod and the pressure edge frame are rotatably connected by connecting bolts. An upper pressure block is installed on the surface of the pressure edge frame on one side close to the vertical center line of the chuck, and a lower pressure block is arranged below the upper pressure block. The sizes of the lower pressure block and the pressure edge frame are matched, and a torsion spring is arranged between the inner side surface of the lower pressure block and the surface of the pressure edge frame.

[0037] Screws are fixed on both sides of the upper surface of the support platform, and a screw sleeve is sleeved on the upper outer side of the screw. The thread sizes of the inner side of the screw sleeve match the outer side of the screw, and the outer wall of the screw sleeve is evenly embedded with telescopic thin plates.

[0038] The connecting end between the telescopic thin plate and the screw sleeve is a spherical structure. A fastening screw passes through the telescopic connection of the telescopic thin plate, and a connecting rod is fixed to the inner side of the outer end of the telescopic thin plate. A measuring rod is embedded in the middle of the telescopic thin plate, and the connecting rod passes through the inside of the end of the measuring rod. A water immersion sensor is installed at one end of the measuring rod.

[0039] A pneumatic folding frame is installed on the outer side of the ring, and the pneumatic folding frame is evenly arranged in a ring shape about the central axis of the ring. A support plate is installed on the lower end of the pneumatic folding frame. An air pipe passes through the upper outer side of the ring, and the air pipe and the pneumatic folding frame are interconnected. The upper end of the air pipe is connected to an air box, and the support plate forms a rotating structure with the ring through the pneumatic folding frame.

[0040] The support plate is evenly provided with threaded holes inside, and a glass rod is installed below the support plate. The upper end of the glass rod is threadedly connected to the threaded hole, and a rubber head is fixed to the bottom end of the glass rod.

[0041] The cleaning assembly includes a rotating column, a supporting tube movably penetrates the upper side of the rotating column, a supporting column is fixedly sleeved on the upper side of the supporting tube, a rotating motor is fixedly installed on the outer wall of the supporting column, a gear a is fixedly installed on the lower end of the output shaft of the rotating motor, an annular rack is fixedly installed on the upper side of the rotating column, and the gear a is meshed and connected with the annular rack, two vertical grooves are provided on the outer wall of the rotating column, circular grooves are provided on the upper sides of the two vertical grooves, movable balls are movably connected in the two circular grooves, connecting columns are fixedly installed on the lower sides of the two movable balls, cleaning rods are fixedly installed on the lower sides of the two connecting columns, and the two cleaning rods are movably connected to the two vertical grooves respectively, an installation groove is provided on the inner wall of the rotating column, the supporting tube is movably connected to the installation groove, and there is a A trapezoidal ring, sliders are fixedly installed on the left and right sides of the trapezoidal ring, and sliding grooves are provided on the left and right walls of the device groove. The two sliders are movably connected with the two sliding grooves respectively. The trapezoidal ring is movably sleeved on the support tube, and a threaded groove is provided on the outer wall of the support tube, and a thread is provided on the inner wall of the trapezoidal ring. The trapezoidal ring is meshed and connected with the support tube. A linkage plate is provided on the lower side of the trapezoidal ring, and a pressure tension spring is fixedly installed on the lower side of the linkage plate. A support plate is fixedly installed on the lower end of the tension spring, and the support plate is fixedly sleeved on the support tube. A trigger strip is movably penetrated on one wall of the two vertical grooves close to each other, and the side of the two trigger strips close to each other fits with the inclined surface of the trapezoidal ring, and an arc plate is fixedly installed on one end of the two trigger strips away from each other, and the two arc plates fit with the two cleaning rods respectively.

[0042] The lower side of the trapezoidal ring is provided with an annular groove, and an annular block is movably connected in both annular grooves, and the lower side of the annular block is fixedly connected to the upper side of the linkage plate;

[0043] The outer wall of the support column is fixedly mounted with a lifting plate, a support strip is movably passed through the lifting plate, and a bottom plate is fixedly mounted at the lower end of the support strip;

[0044] A vertical bar is fixedly installed on the lower side of the lifting plate, a rubber ring is fixedly installed on the lower end of the vertical bar, and the rubber ring is movably sleeved on the outer side of the rotating column;

[0045] The rotating column is provided with a linkage groove, in which a bevel gear is movably connected, and the bevel gear is fixedly sleeved on the support tube;

[0046] The bevel gear is meshed with two gears b, and the upper sides of the two gears b are movably penetrated by inclined rods, and the two inclined rods are fixedly connected to the upper wall of the linkage groove;

[0047] The upper walls of the two circular grooves are provided with two connecting grooves, the two connecting grooves are connected with the linkage groove, and movable columns are movably connected in the two connecting grooves;

[0048] The two movable columns are fixedly connected to the two movable balls respectively, and the two movable columns are fixedly sleeved with gears c;

[0049] A transverse groove is provided on the lower side of the rotating column, and two telescopic tubes are movably connected in the transverse groove;

[0050] The two telescopic tubes are both fixedly connected to the supporting tube, and water outlet grooves are provided on the upper sides of the two telescopic tubes.

[0051] The first stirring mechanism and the second stirring mechanism have the same structure.

[0052] Compared with the prior art, the advantages and positive effects of the present invention are:

[0053] 1. The present invention utilizes the above method to prepare a sterile compound multi-adhesive product, which is simple and effective, and uses integrated equipment to conduct multiple groups of experiments on it, making the entire design more efficient and saving labor accordingly. DETAILED DESCRIPTION

[0054] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0055] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0056] The present invention provides a method for preparing a sterile compound multi-adhesive product, wherein the sterile compound multi-adhesive product specifically comprises the following components:

[0057] Components Number of copies White Vaseline 50 servings Yellow Vaseline 45 servings Light liquid paraffin 20 servings Sterile Material A 5 servings Sterile Material B 4 servings

[0058] The specific preparation method is as follows:

[0059] A. Preheat and melt white vaseline and yellow vaseline respectively to obtain flowable white vaseline and yellow vaseline;

[0060] B. Then, put white vaseline, yellow vaseline and light liquid paraffin into three beakers A respectively for sterilization;

[0061] C. Add white vaseline and yellow vaseline into two beakers B respectively and heat them in a water bath to keep them warm;

[0062] D. Place light liquid paraffin in beaker B, add sterile raw material A into beaker B, and stir to mix to obtain homogenized liquid A;

[0063] E. Add the weighed sterile material B to the homogenized solution, and mix and stir the standby main drug suspension;

[0064] F. Cool the white vaseline and yellow vaseline in beaker B, add them to the standby main drug suspension, and mix and stir them to obtain a sterile compound multi-viscosity product.

[0065] The sterilization temperature in step B is 145-160° C. and the duration is 1-3 hours.

[0066] The water bath heating temperature in step C is 80-90°C.

[0067] The sterile material A is any one of lidocaine hydrochloride, pramoxine hydrochloride, procaine hydrochloride, tetracaine hydrochloride, prilocaine hydrochloride and ropivacaine hydrochloride.

[0068] Its sterile material B is antibiotics, such as polymyxin B sulfate, neomycin sulfate and bacitracin (bacitracin zinc). It is best to talk about the superordinate concept here.

[0069] The temperature in step F is lowered to 45-50° C., and the temperature is kept constant for 8-12 minutes after the temperature is lowered.

[0070] The mixing and stirring time in step F is 40-50 minutes, and the rotation speed is 1500r / min.

[0071] According to the above method, the following embodiments are prepared

[0072] Embodiment 1:

[0073] 45 g of white vaseline, 35 g of yellow vaseline and 20 g of light liquid paraffin were placed in a beaker and sterilized at 150°C for 3 hours.

[0074] In a biosafety cabinet, add white vaseline and yellow vaseline to a beaker and heat in a water bath at 80-90°C;

[0075] In a biosafety cabinet, place light liquid paraffin in another beaker, add 4 g of sterile lidocaine hydrochloride, and stir to mix to form a homogenized solution;

[0076] In a biological safety cabinet, 59.6 mg of polymyxin B sulfate, 536.8 mg of neomycin sulfate and 657.8 mg of bacitracin aseptic raw materials were added to the homogenized solution in sequence, and stirred to mix as the main drug suspension for later use.

[0077] Cool white vaseline and yellow vaseline to 45-50℃ and keep for 10 minutes, add the above main drug suspension, keep the water bath temperature at 45-50℃ and mix and stir for 45 minutes, speed: 1500r / min;

[0078] Fill into pharmaceutical aluminum tube while hot, fold and seal the end, and place at room temperature.

[0079] Labeled and put into storage, batch number SE-2022-012-ZJ-230601-01

[0080] Embodiment 2:

[0081] 45 g of white vaseline, 35 g of yellow vaseline and 20 g of light liquid paraffin were placed in a beaker and sterilized at 150°C for 3 hours.

[0082] In a biosafety cabinet, add white vaseline and yellow vaseline to a beaker and heat in a water bath at 80-90°C;

[0083] In a biosafety cabinet, place light liquid paraffin in another beaker, add 4 g of sterile lidocaine hydrochloride, and stir to mix to form a homogenized solution;

[0084] In a biological safety cabinet, 59.6 mg of polymyxin B sulfate, 536.8 mg of neomycin sulfate and 657.8 mg of bacitracin zinc aseptic raw materials were added to the above homogenized solution in sequence, and stirred to mix as the main drug suspension for later use.

[0085] Cool white vaseline and yellow vaseline to 45-50℃ and keep for 10 minutes, add the above main drug suspension, keep the water bath temperature at 45-50℃ and mix and stir for 45 minutes, speed: 1500r / min;

[0086] Fill into pharmaceutical aluminum tube while hot, fold and seal the end, and place at room temperature.

[0087] Labeled and put into storage, batch number SE-2022-012-ZJ-230601-02

[0088] Embodiment 3:

[0089] 45 g of white vaseline, 35 g of yellow vaseline and 20 g of light liquid paraffin were placed in a beaker and sterilized at 150°C for 3 hours.

[0090] In a biosafety cabinet, add white vaseline and yellow vaseline to a beaker and heat in a water bath at 80-90°C;

[0091] In a biosafety cabinet, light liquid paraffin is placed in another beaker, 4 g of tetracaine hydrochloride sterile raw material is added, and the mixture is stirred to form a homogenized solution;

[0092] In a biological safety cabinet, 59.6 mg of polymyxin B sulfate, 536.8 mg of neomycin sulfate and 657.8 mg of bacitracin zinc aseptic raw materials were added to the above homogenized solution in sequence, and stirred to mix as the main drug suspension for later use.

[0093] Cool white vaseline and yellow vaseline to 45-50℃ and keep for 10 minutes, add the above main drug suspension, keep the water bath temperature at 45-50℃ and mix and stir for 45 minutes, speed: 1500r / min;

[0094] Fill into pharmaceutical aluminum tube while hot, fold and seal the end, and place at room temperature.

[0095] Labeled and put into storage, batch number SE-2022-012-ZJ-230601-03

[0096] Embodiment 4:

[0097] 45 g of white vaseline, 35 g of yellow vaseline and 20 g of light liquid paraffin were placed in a beaker and sterilized at 150°C for 3 hours.

[0098] In a biosafety cabinet, add white vaseline and yellow vaseline to a beaker and heat in a water bath at 80-90°C;

[0099] In a biosafety cabinet, light liquid paraffin is placed in another beaker, 4 g of tetracaine hydrochloride sterile raw material is added, and the mixture is stirred to form a homogenized solution;

[0100] In a biological safety cabinet, 59.6 mg of polymyxin B sulfate, 536.8 mg of neomycin sulfate and 657.8 mg of bacitracin zinc aseptic raw materials were added to the above homogenized solution in sequence, and stirred to mix as the main drug suspension for later use.

[0101] Cool white vaseline and yellow vaseline to 45-50℃ and keep for 10 minutes, add the above main drug suspension, keep the water bath temperature at 45-50℃ and mix and stir for 45 minutes, speed: 1500r / min;

[0102] Fill into pharmaceutical aluminum tube while hot, fold and seal the end, and place at room temperature.

[0103] Labeled and put into storage, batch number SE-2022-012-ZJ-230601-04.

[0104] Sterility test results:

[0105] Take 1 tube (10g) of this product, add sterile pH 7.0 sodium chloride-peptone buffer to 100ml, shake repeatedly, and use it as the test solution. Staphylococcus aureus is used as the control solution. Place it in an incubator and observe for 14 days.

[0106] Information on strains used in research

[0107]

[0108]

[0109] 5. Judgment criteria: The positive control has good growth, the negative control has no bacterial growth, and the sample tube is clarified to confirm the sterile growth. 6. Conclusion: Comply with regulations □Not comply with regulations

[0110]

[0111] 5. Judgment criteria: The positive control has good growth, the negative control must have no bacterial growth, and the sample tube is clarified to confirm sterile growth.

[0112] VI. Conclusion: □Complies with regulations □Does not comply with regulations

[0113]

[0114] 1. Subculture and confirmation of bacterial strains: Take a loop of bacterial strain stored in 50 / 50% BHI-glycerol at -80℃, streak it on a TSA plate, and culture it at 36℃ for 20-24 hours. Then, take a single colony and streak it on a TSA plate again. Take a single colony and confirm the bacterial strain by MALDI-TOF MS.

[0115] 2. Treatment and preparation of ointment preparations: Weigh 2.56 g of different ointment preparations for external use into 50 ml centrifuge tubes, add 40 ml PBS (pH = 7.4)-0.1% Tween 80, and shake in a water bath at 50°C until the sample is fully dissolved and mixed to obtain 64 mg / ml of the test substance for use.

[0116] 3. MICs test of different preparations: The MIC of different ointment external preparations was determined by agar dilution method recommended by CLSI. The specific method is as follows: Take 5 ml of the test substance prepared in step 2 with a concentration of 64 mg / ml, add MH agar to 20 ml, prepare 16 mg / ml MH agar plate, and then dilute the sample with PBS (pH=7.4)-0.1% Tween 80 to prepare MH agar plates containing preparations with concentrations of 8, 4, 2, 1, 0.5, 0.125, 0.0625, 0.031, 0.015 and 0.007 mg / ml, respectively. Use a sterile cotton swab to take the second-generation culture of the fresh TSA plate, add it to sterile physiological saline, and prepare a bacterial suspension with a McFarland turbidity of 1.5-2.0. Dilute the bacterial suspension 1:10 in physiological saline, take 1 μl of the diluted bacterial suspension and inoculate it on the surface of MH agar using a multi-point inoculator, incubate at 36°C for 20-24 hours, and observe with the naked eye whether there is microbial growth on MH agar. Record the minimum no-growth concentration of each strain for each preparation one by one, which is the MIC of the preparation for the strain.

[0117] result

[0118] As can be seen from Tables 4 and 5, the 50th percentile of the MIC of the homemade sterile ointment preparations against 75 bacterial strains of 11 species was 0.5 mg / ml. When the concentration reached 4 mg / ml, it could effectively inhibit 73 bacterial strains of the 11 species tested. Statistical analysis found that there was no significant difference in the MICs of the homemade sterile ointment preparations (P>0.05).

[0119] There was a significant difference between the homemade sterile ointment preparation and Funo (P < 0.01). The difference with Funo appeared in Staphylococcus (n = 15), Escherichia coli (n = 5), Pseudomonas aeruginosa (n = 5), Klebsiella pneumoniae (n = 5), Enterococcus (n = 1), Streptococcus pyogenes (n = 1) and Bacillus subtilis (n = 1). The in vitro MIC value of the homemade sterile ointment preparation was lower than that of the marketed product, and the antibacterial effect was higher than that of the marketed product.

[0120] MICs test results of different ointment preparations against different species of bacteria

[0121]

[0122]

[0123]

[0124]

[0125] With respect to the above method, the present invention also provides an integrated device for preparing the above product.

[0126] The present invention provides an integrated device for preparing a sterile compound multi-adhesive product.

[0127] The present invention provides an integrated device for preparing sterile compound multi-viscosity products, including a main workbench, a new type of laminar flow hood is arranged above the main workbench, the main workbench and the new type of laminar flow hood are connected and fixed by a fixed square tube, a sterilization component, a preheating component, and a mixing and stirring component are arranged from top to bottom between the main workbench and the new type of laminar flow hood, three storage tanks are arranged on both sides of the new type of laminar flow hood, a supporting fixing rod is arranged on the fixed square tube, and a first new type of stirring mechanism and a second new type of stirring mechanism are arranged on the supporting fixing rod. It is not difficult to see from the above description that the main workbench serves as the supporting component of the entire device, and its new type of laminar flow hood is a protective device that protects the entire device from external bacteria and viruses and internal and external air circulation, and a sterilization component, a preheating component, and a mixing and stirring component are arranged from top to bottom.

[0128] The following is a detailed description of the composition of the above three important components.

[0129] The sterilization component comprises a rotating disc, on which three new beakers A are arranged. The bottom of the new beaker A is a conical structure. A heating jacket is arranged on the outside of the new beaker A, and a small vibrating rod is arranged on the bottom. A motor 3 is installed in the center of the base 1; the motor 3 is fixedly connected to the disc 4; a circle of baffle 5 is erected between the base 1 and the disc 4; two symmetrical vertical rods 6 are erected on the edge of the disc 4; a threaded hole 7 is opened in the upper middle part of the vertical rod 6; a threaded rod 8 is threadedly connected to the threaded hole 7; a semicircular supporting plate 9 is connected to one end of the threaded rod 8; a rotating handle 10 is welded to the other end of the threaded rod 8; a beaker 2 is placed on the disc 4; three buttons 11 are arranged on the base 1; the base 1 is circular; the three buttons 11 are electrically connected to the motor 3; the inner side of the baffle 5 is made of rubber material; a gap is left between the disc 4 and the baffle 5; pour the mixed liquid into the beaker 2, rotate the rotating handle 10 to drive the threaded rod 8 to approach the beaker 2 through the threaded hole 7 on the vertical rod 6, and it cannot be fixed too tightly to leave a certain gap, and then press the button 11 to make the motor 3 starts working, the motor 3 drives the disc 4 to rotate, and the disc 4 drives the beaker 2 to rotate. The three buttons 11 are respectively forward, power switch, and reverse. The corresponding button 11 is pressed as needed. In order to make the reaction more complete and shorten the reaction time, a combination of forward and reverse rotation can be adopted; the motor 3 is installed in the center of the base 1 of the present invention, and the motor 3 is used to replace manpower, which can not only make the reaction complete, but also liberate labor; a semicircular support plate 9 is connected to one end of the threaded rod 8 to prevent the beaker 2 from falling when the beaker 2 rotates; the inner side of the baffle 5 is made of rubber material, which effectively reduces the collision force between the beaker 2 and the baffle 5 when the beaker 2 shakes; a gap is left between the disc 4 and the baffle 5, which will not become a resistance when the disc 4 rotates, and there is no need to worry that the disc 4 will be unevenly stressed and seriously tilted.

[0130] The invention comprises a main workbench, a novel laminar flow hood is arranged above the main workbench, the main workbench and the novel laminar flow hood are connected and fixed by a fixed square tube, a sterilization component, a preheating component, and a mixing and stirring component are arranged in sequence from top to bottom between the main workbench and the novel laminar flow hood, three storage tanks are arranged on both sides of the novel laminar flow hood, a supporting fixing rod is arranged on the fixed square tube, and a first novel stirring mechanism and a second novel stirring mechanism are arranged on the supporting fixing rod

[0131] The sterilization component, preheating component and mixing component are fixed on the main workbench through connecting rods.

[0132] The sterilization component comprises a rotating disc, on which three new beakers A are arranged. The bottom of the new beaker A is a conical structure. A heating jacket is arranged on the outside of the new beaker A, and a small vibrating rod is arranged on the bottom.

[0133] The preheating component includes a preheating part and a cooling part.

[0134] The preheating part includes the outer wall of the beaker, the inner wall of the beaker, the auxiliary cup wall, a closed cooling pipe, a circulating cooling pipe, a circulating pump connection port, a water pipe connection port, and a cup wall sliding sleeve. The inner side of the outer wall of the beaker is connected to the inner wall of the beaker, and the lower part of the outer wall of the beaker is connected to multiple closed cooling pipes. There is cold water inside the closed cooling pipe, and the inner wall of the beaker is connected to the circulating cooling pipe. There is circulating cold water inside the circulating cooling pipe. The left side of the circulating cooling pipe has a circulating pump connection port, and the left side of the circulating pump connection port passes through the outer wall of the beaker to connect the circulating pump. The right side of the circulating cooling pipe has a water pipe connection port, and the water pipe connection port passes through the outer wall of the beaker to connect the water pipe. The outer side of the inner wall of the beaker has a cup wall sliding sleeve, and the outer side of the cup wall sliding sleeve is connected to the circulating cooling pipe. The inner side of the cup wall sliding sleeve is connected to the auxiliary cup wall through a ring screw, and the auxiliary cup wall slides in the cup wall sliding sleeve, and the auxiliary cup wall has fastening grooves on both sides. The fastening chute is symmetrically arranged, and has a plurality of positioning threaded holes inside the fastening chute, and the positioning threaded holes are evenly distributed inside the fastening chute. There are fixed connection ears on both sides of the outer wall of the beaker, and the fixed connection ears are symmetrically arranged, and the upper part of the fixed connection ears is connected to the cup wall fixing plate through a rotating shaft. The cup wall fixing plate rotates on the rotating shaft, and has a fixed circular hole inside the cup wall fixing plate. The two sides of the auxiliary cup wall are connected to the cup wall fixing plate through circular ring screws, and the auxiliary cup wall slides in the cup wall fixing plate. The circular ring screw passes through the fixed circular hole to connect the positioning threaded hole, and the right side of the cup wall fixing plate has a fastening protrusion, and the fastening protrusion is adapted to the fastening chute, and the fastening protrusion slides in the fastening chute. When the auxiliary cup wall slides in the circulating cooling tube, the height of the auxiliary cup wall can be adjusted according to actual conditions. Under the action of the auxiliary cup wall, liquid splashing caused by heating and boiling can be effectively avoided, which plays a good protective role for the staff and makes the overall structure more reasonable. At the same time, the water inside the circulating cooling tube is circulating cold water. Under the action of the circulating cooling tube, the solution inside the outer wall of the beaker is accelerated to cool down, making the overall structure more reasonable.

[0135] The cooling part includes a cooling base, a cooling fan is arranged on the cooling base, a rotating motor for driving the cooling fan to rotate is arranged in the cooling base, a manual switch for controlling the start and stop of the rotating motor is arranged on the outer wall of the cooling base, baffles are arranged around the top of the cooling base, the baffles form a rectangular frame, a cooling inner frame is in the rectangular frame, the cooling inner frame is a mesh structure, a placement outer edge extending outward is arranged on the top of the cooling inner frame; a plurality of partitions are arranged in the cooling inner frame; the cooling inner frame is made of plastic material.

[0136] The mixing and stirring assembly comprises a base and a frame, wherein a central motor is installed in the middle of the interior of the base, and the shaft end of the central motor is connected to the central rotating shaft, a support is fixed to the upper end of the central rotating shaft, and a cushion ball is arranged between the support and the base, unit motors are installed on both the left and right sides of the interior of the support, and the shaft end of the unit motor is connected to the unit rotating shaft, and heat dissipation nets are arranged on both the left and right sides of the unit motor, stirring motors are arranged on both the left and right sides above the frame, and the bottom end of the frame is welded to the upper surface of the base, the shaft end of the stirring motor is connected to the stirring shaft, and a ferrule is arranged on the lower outer side of the stirring shaft.This part is connected to the power plug through a wire, a control panel is installed on one side of the base, a display screen, control buttons and indicator lights are installed on one side of the control panel 2, a temperature controller and a PLC controller are installed inside the control panel, a beaker placement slot is installed on the top of the base, a heater 9 is installed at the bottom of the beaker placement slot 3, an electric push rod 8 is installed on one side of the beaker placement slot 3, the bottom of the electric push rod 8 is installed inside the base 1, a top plate 6 is installed on the top of the electric push rod 8, a rotating motor 7 is installed at the top center of the top plate 6, a rotating disc 11 is installed at the bottom center of the top plate 6, two stirring rods 5 are installed at the bottom of the rotating disc 11, and the bottom of the stirring rod 5 A temperature sensor 4 is installed on the top, a distance sensor 13 is installed on one side of the rotating disk 11, and a reducer 12 is installed inside the top plate 6; the control panel 2, the temperature sensor 4, the rotating motor 7, the electric push rod 8, the heater 9 and the distance sensor 13 are all electrically connected to the PLC controller 15 to facilitate signal transmission; the electric push rod 8 is composed of a driving motor, a reduction gear, a screw, a nut, a guide sleeve, a push rod, a slide seat, a spring, a housing, a turbine and a micro-control switch to facilitate the top plate 6 to move up and down; the rotating motor 7 is movably connected to the reducer 12 through gears, and the reducer 12 is movably connected to the rotating disk 11 through gears to facilitate the rotation. The disc 11 rotates; further, the rotating motor 7 is movably connected to the top plate 6 by bolts, which is convenient for disassembly and is conducive to the maintenance and maintenance of the rotating motor 7; further, the stirring rod 5 is movably connected to the rotating disc 11 by bolts, which is convenient for disassembly and is conducive to the maintenance and maintenance of the stirring rod 5; Working principle: when working, a beaker containing a mixture of solid and solution is placed on the beaker placement slot 3, and the PLC controller 15 controls the electric push rod 8 to run by operating the control panel 2, driving the top plate 6 to run, and the distance sensor 13 is convenient for real-time monitoring of the distance between the top plate 6 and the base 1. When the distance between the top plate 6 and the base 1 reaches the preset value of the PLC controller 15, The PLC controller 15 controls the electric push rod 8 to stop running, and controls the heater 9 to run by operating the control panel 2. The temperature sensor 4 at the bottom of the stirring rod 5 can monitor the changes in the internal temperature of the beaker in real time, and transmit the collected signal to the PLC controller 15. When the temperature reaches the preset value of the PLC controller 15, the PLC controller 15 controls the temperature controller 14 to run, so that the internal temperature of the beaker remains stable. Then, the PLC controller 15 controls the rotating motor 7 to run by operating the control panel 2, and then drives the stirring rod 5 to run, so as to stir the solution, thereby facilitating precise temperature control, increasing the solid dissolution rate, and helping to improve work efficiency.

[0137] The support platform forms a rotating structure with the base through a central rotating shaft and a central motor, and the unit motors are symmetrical about the symmetry center line of the support platform. A chuck is fixed to the upper end of the unit rotating shaft, and a hydraulic cylinder is arranged at the inner bottom of the chuck. A hydraulic oil pipe is connected to a port on the side of the hydraulic cylinder away from the vertical center line of the chuck.

[0138] The inner wall of the chuck is evenly and evenly provided with hydraulic telescopic rods in a ring shape, and the hydraulic telescopic rods are all connected to the hydraulic oil pipes. A rubber pad is bonded to the side of the hydraulic telescopic rod close to the vertical center line of the chuck. A cup holder is fixed on the upper inner side of the chuck, and a protrusion is provided in the middle of the upper surface of the cup holder. The protrusion is a truncated cone structure, and anti-slip pads are evenly provided around the protrusion.

[0139] A limiting pressure ring is fixed on the upper surface of the support, and the inner side of the limiting pressure ring fits the edge of the upper surface of the chuck. A fixing rod is fixed on the upper surface of the limiting pressure ring, and two fixing rods are symmetrically arranged about the vertical center line of the chuck.

[0140] A pressure edge frame is installed above the fixed rod, and the fixed rod and the pressure edge frame are rotatably connected by connecting bolts. An upper pressure block is installed on the surface of the pressure edge frame close to the vertical center line of the chuck, and a lower pressure block is arranged below the upper pressure block. The dimensions of the lower pressure block and the pressure edge frame are matched, and a torsion spring is arranged between the inner side surface of the lower pressure block and the surface of the pressure edge frame.

[0141] Screws are fixed on both sides of the upper surface of the support platform, and a screw sleeve is sleeved on the upper outer side of the screw. The thread sizes of the inner side of the screw sleeve match the outer side of the screw, and the outer wall of the screw sleeve is evenly embedded with telescopic thin plates.

[0142] The connecting end between the telescopic thin plate and the screw sleeve is a spherical structure. A fastening screw passes through the telescopic connection of the telescopic thin plate, and a connecting rod is fixed to the inner side of the outer end of the telescopic thin plate. A measuring rod is embedded in the middle of the telescopic thin plate, and the connecting rod passes through the inside of the end of the measuring rod. A water immersion sensor is installed at one end of the measuring rod.

[0143] A pneumatic folding frame is installed on the outer side of the ring, and the pneumatic folding frame is evenly arranged in a ring shape about the central axis of the ring. A support plate is installed on the lower end of the pneumatic folding frame. An air pipe passes through the upper outer side of the ring, and the air pipe and the pneumatic folding frame are interconnected. The upper end of the air pipe is connected to an air box, and the support plate forms a rotating structure with the ring through the pneumatic folding frame.

[0144] The support plate is evenly provided with threaded holes inside, and a glass rod is installed below the support plate. The upper end of the glass rod is threadedly connected to the threaded hole, and a rubber head is fixed to the bottom end of the glass rod.

[0145] The cleaning assembly comprises a rotating column, a supporting tube movably penetrates the upper side of the rotating column, a supporting column is fixedly sleeved on the upper side of the supporting tube, a rotating motor is fixedly installed on the outer side wall of the supporting column, a gear a is fixedly installed on the lower end of the output shaft of the rotating motor, an annular rack is fixedly installed on the upper side of the rotating column, the gear a is meshed and connected with the annular rack, two vertical grooves are provided on the outer side wall of the rotating column, circular grooves are provided on the upper sides of the two vertical grooves, movable balls are movably connected in the two circular grooves, connecting columns are fixedly installed on the lower sides of the two movable balls, cleaning rods are fixedly installed on the lower sides of the two connecting columns, the two cleaning rods are movably connected to the two vertical grooves respectively, and the inner side of the rotating column is provided with a plurality of movable balls, and the two movable balls are fixedly installed on the lower sides of the two connecting columns. The wall is provided with an installation groove, the support tube is movably connected with the installation groove, a trapezoidal ring is movably connected in the installation groove, sliders are fixedly installed on the left and right sides of the trapezoidal ring, sliding grooves are provided on the left and right walls of the installation groove, the two sliders are movably connected with the two sliding grooves respectively, the trapezoidal ring is movably sleeved on the support tube, a threaded groove is provided on the outer wall of the support tube, a thread is provided on the inner wall of the trapezoidal ring, the trapezoidal ring is meshed with the support tube, a linkage plate is provided on the lower side of the linkage plate, a pressure tension spring is fixedly installed on the lower side of the linkage plate, a support plate is fixedly installed on the lower end of the tension spring, the support plate is fixedly sleeved on the support tube, a trigger strip is movably penetrated through one wall of the two vertical grooves close to each other, and the two The side of the trigger bars close to each other fits with the inclined surface of the trapezoidal ring, and the ends of the two trigger bars away from each other are fixedly installed with arc plates, and the two arc plates fit with the two cleaning rods respectively; an annular groove is provided on the lower side of the trapezoidal ring, and annular blocks are movably connected in the two annular grooves, and the lower side of the annular block is fixedly connected to the upper side of the linkage plate; a lifting plate is fixedly installed on the outer wall of the support column, and a support bar movably penetrates the lifting plate, and a bottom plate is fixedly installed at the lower end of the support bar; a vertical bar is fixedly installed on the lower side of the lifting plate, and a rubber ring is fixedly installed at the lower end of the vertical bar, and the rubber ring is movably sleeved on the outer side of the rotating column; a linkage groove is provided in the rotating column, and a conical Gear, the bevel gear is fixedly sleeved on the support tube; two gears b are meshed and connected on the bevel gear, and inclined rods are movably penetrated on the upper sides of the two gears b, and the two inclined rods are fixedly connected to the upper wall of the linkage groove; two connecting grooves are provided on the upper walls of the two circular grooves, and the two connecting grooves are connected with movable columns movably; the two movable columns are respectively fixedly connected with two movable balls, and gears c are fixedly sleeved on the two movable columns; a transverse groove is provided on the lower side of the rotating column, and two telescopic tubes are movably connected in the transverse groove; the two telescopic tubes are fixedly connected to the support tube, and water outlet grooves are provided on the upper sides of the two telescopic tubes.The utility model comprises a circular cleaning tank 1, a cleaning disc 2 clamped with the circular cleaning tank 1 and a fixed cover 3, wherein the circular cleaning tank 1 is a cylindrical hollow structure with an opening upward, and a fixed clamping groove 4 is symmetrically provided along the length of the opening edge of the upper end of the circular cleaning tank 1, the lower end surface of the cleaning disc 2 is provided with two or more transmission shafts 5 penetrating the cleaning disc 2, and a synchronous wheel 6 is provided on the transmission shaft 5 located at the lower end surface of the cleaning disc 2, the upper end surface of the cleaning disc 2 is provided with a seat bearing 7 for fixing the transmission shaft 5, and a waterproof motor 8 is provided on the upper end surface of the cleaning disc 2, and the waterproof motor 8 is electrically connected to the power supply arranged on the circular cleaning tank 1 through a wire, and the output shaft of the waterproof motor 8 is transmission-connected to the synchronous wheel 6 through a synchronous belt, and a cleaning brush 9 is installed on the transmission shaft 5 located at the upper end of the cleaning disc 2, and the cleaning brush 9 is composed of a fixing rod 9a and a spiral brush 9b distributed on the fixing rod 9a, the bottom end of the fixing rod 9a is provided with a thread, and the fixing rod 9a is connected to the transmission shaft located at the upper end of the cleaning disc 2 through a thread. The threaded connection of the threaded hole opened at the upper end of 5 is not only convenient for replacing the cleaning brush 9b, but also can play a role in guiding, so that the medicine flows into the circular cleaning tank 1 along with the brush 9b, avoiding the medicine residue from remaining on the brush 9b. The two sides of the cleaning disc 2 are provided with limit blocks 10 that are engaged with the fixed card slot 4, and the cleaning disc 2 is provided with a shaped hose 11 for flushing the experimental tools at the edge of the cleaning brush 9. The cleaning disc 2 is provided with a water seepage hole, and the fixed cover 3 is opened on the side facing the cleaning disc 2. A circular limiting groove 12 is provided which is compatible with the cleaning brush 9. The fixed cover 3 is made of rubber material so that the fixed cover 3 has a certain elasticity. Long strip holes 14 are symmetrically opened around the outer side of the circular limiting groove 12 along the radial extension direction. The part between two adjacent long strip holes 14 of the same circular limiting groove 12 can be recessed toward one side of the fixed cover 3. Therefore, it can be used to fix test tubes or cylindrical beakers of different sizes, and limiting blocks 13 2 which are engaged with the fixed slot 4 are provided on both sides of the fixed cover 3.Working principle: when in use, insert the test tube or beaker onto the cleaning brush 9, and then fix the test tube or beaker on the cleaning disc 2. At the same time, ensure that the qualitative hose 11 is located in the inner cavity of the test tube or beaker. Then, the other end of the qualitative hose 11 is connected to a water pump through a main pipe, which is convenient for flushing the inner wall of the test tube or beaker during the cleaning process. Then, align the circular limiting groove 12 on the fixed cover 3 with the test tube or beaker on the cleaning disc 2, and clamp the limiting card block 2 13 on the fixed cover 3 in the fixed card groove 4 on the circular cleaning groove 1. Then, start the waterproof motor 8, so that the waterproof motor 8 drives the transmission shaft 5 to rotate, and then the cleaning brush 9 rotates in the inner cavity of the test tube or beaker to clean the test tube or beaker. The cleaning brush 9 is composed of a fixed rod 9a and a spiral brush 9b distributed on the fixed rod 9a. The bottom end of the fixed rod 9a is provided with a thread, and the fixed rod 9a is connected to the position through the thread. The threaded hole opened at the upper end of the transmission shaft 5 at the upper end of the cleaning disc 2 is threadedly connected, and the cleaning brush 9 can be replaced according to the size of the beaker or test tube, so that cleaning is cleaner, and it can also play a role of diversion, so that the medicine flows into the circular cleaning tank 1 through the seepage hole along the brush 9b, avoiding the residue of the medicine remaining on the brush 9b. Among them, the outer side of the circular limiting groove 12 is symmetrically provided with long holes 14 along the extension direction of its radius. The part between two adjacent long holes 14 of the same circular limiting groove 12 can be recessed toward one side of the fixed cover 3. Therefore, it can be used to fix test tubes or cylindrical beakers of different sizes (referring to the area between the radius of the circular limiting groove 12 and the radius of the sum of the radius of the circular limiting groove 12 and the length of the long hole 13). In addition, since the cleaning disc 2 and the fixed cover 3 are all snap-fitted to the circular cleaning tank 1, it is convenient to disassemble and clean.

[0146] The first stirring mechanism and the second stirring mechanism have the same structure, including an upper base 1, a rotating assembly 2 is fixedly installed in the upper base 1, and two columns 3 are fixedly installed on the upper end surface of the upper base 1, and a strip groove 4 is provided in the column 3, and a cover plate 5 is movably installed between the two columns 3, and two sliders 6 are fixedly installed on the sides of the cover plate 5, and the sliders 6 are snap-fitted and connected with the strip groove 4, the sliders 6 are symmetrically arranged about the center of the cover plate 5, and a snap-fitting sliding connection structure is formed between the sliders 6 and the strip groove 4, which increases the stability of the cover plate 5 when it moves, and an annular groove 7 is provided on the lower end surface of the cover plate 5, and two first support rods 8 are snap-fitted and installed in the annular groove 7, and the lower ends of the two first support rods 8 are connected by a connecting disc 9, and a magnetic rotor 10 is fixedly installed on the lower end surface of the connecting disc 9, and the first support rod 8 and the annular groove 7 form a snap-fitting sliding connection structure, and the first support rod 8 and the connecting disc 9 are welded as an integrated structure, which does not affect the synchronous rotation of the connecting disc 9 driven by the magnetic rotor 10. Four telescopic rods 11 are fixedly mounted on the inner wall of the disk 9, and a fixed shaft 12 is fixedly mounted on the inner side of the end of the telescopic rod 11, and a stirring rod 13 is fixedly mounted on the upper end surface of the output shaft of the telescopic rod 11, and the four fixed shafts 12 are connected by four connecting rods 14. The telescopic rods 11 are distributed at equal angles on the inner wall of the connecting disk 9, and the side section of the connecting disk 9 is a concave structure, which is conducive to the telescopic rod 11 driving the stirring rod 13 to stir evenly, and the connecting rod 14 is relatively rotatably connected to the fixed shaft 12, and There is a one-to-one correspondence between the fixed shaft 12 and the telescopic rod 11, so that the operation of the connecting rod 14 is not hindered by the fixed shaft 12. A second support rod 15 is fixedly installed in the middle of the lower end surface of the cover plate 5, and a two-way cam 16 is fixedly installed at the lower end of the second support rod 15. The horizontal plane height of the two-way cam 16 is equal to the horizontal plane height of the connecting rod 14, and the outer wall of the two-way cam 16 is fitted and connected with the connecting rod 14, so that the telescopic rod 11 can be extended and retracted by limiting the connecting rod 14 through the two-way cam 16. The present invention drives the slider 6 to slide in the strip groove 4 through the cover plate 5, so that the movement of the cover plate 5 is more stable, and the upper end of the beaker is sealed by the cover plate 5, which increases the heat preservation effect and prevents the solution from splashing out to affect the experimental process. The present invention drives the telescopic rod 11 to operate through the fitting connection between the connecting rod 14 and the two-way cam 16, so that the four stirring rods 13 rotate at different radii, enhance the stirring effect, and improve work efficiency.By sliding the slider 6 in the strip groove 4, the lifting of the cover plate 5 is more stable, which is convenient for placing the beaker. The rotating component 2 drives the magnetic rotor 10 to rotate, so that the connecting disc 9 rotates synchronously. By sliding the first support rod 8 in the annular groove 7, the operation of the connecting disc 9 is convenient. Since the two-way cam 16 is fixedly connected to the cover plate 5 through the second support rod 15, the two-way cam 16 remains in a stationary state. The rotation of the connecting disc 9 causes the four connecting rods 14 to be limited by the two-way cam 16, thereby driving the four telescopic rods 11 to continuously perform telescopic movements, and at the same time drives the four stirring rods 13 to move horizontally, thereby increasing the stirring effect of the equipment. A slot is provided at the bottom of the connecting disc 9 to prevent residual solution when the cover plate 5 drives the connecting disc 9 to be taken out of the beaker.

[0147] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A method for preparing a sterile compound multi-adhesive product, characterized in that: The sterile compound multi-adhesive product specifically includes the following components: The specific preparation method is as follows: A. Preheat and melt white vaseline and yellow vaseline respectively to obtain flowable white vaseline and yellow vaseline; B. Then, put white vaseline, yellow vaseline and light liquid paraffin into three beakers A respectively for sterilization; C. Add white vaseline and yellow vaseline into two beakers B respectively and heat them in a water bath to keep them warm; D. Place light liquid paraffin in beaker B, add sterile raw material A into beaker B, and stir to mix to obtain homogenized liquid A; E. Add the weighed sterile material B to the homogenized solution, and mix and stir the standby main drug suspension; F. Cool the white vaseline and yellow vaseline in beaker B, add them to the standby main drug suspension, and mix and stir them to obtain a sterile compound multi-viscosity product.

2. A method for preparing a sterile compound multi-adhesive product according to claim 1, characterized in that: The sterilization temperature in step B is 145-160°C and the duration is 1-3h.

3. A method for preparing a sterile compound multi-adhesive product according to claim 2, characterized in that: The water bath heating temperature in step C is 80-90°C.

4. A method for preparing a sterile compound multi-adhesive product according to claim 3, characterized in that: The sterile material A is any one of lidocaine hydrochloride, pramoxine hydrochloride, procaine hydrochloride, tetracaine hydrochloride, prilocaine hydrochloride, and ropivacaine hydrochloride.

5. A method for preparing a sterile compound multi-adhesive product according to claim 4, characterized in that: The sterile material B is an external antibiotic, such as polymyxin B sulfate, neomycin sulfate and bacitracin (bacitracin zinc). It is best to talk about the general concept here.

6. A method for preparing a sterile compound multi-adhesive product according to claim 5, characterized in that: The temperature in step F is lowered to 45-50° C., and the temperature is kept constant for 8-12 minutes after the temperature is lowered.

7. A method for preparing a sterile compound multi-adhesive product according to claim 6, characterized in that: The mixing and stirring time in step F is 40-50 minutes, and the rotation speed is 1500 r / min.

8. An integrated device for preparing the sterile composite multi-adhesive product according to any one of claims 1 to 6, characterized in that: The utility model comprises a main workbench, a novel laminar flow hood is arranged above the main workbench, the main workbench and the novel laminar flow hood are connected and fixed by a fixed square tube, a sterilization component, a preheating component and a mixing and stirring component are arranged in sequence from top to bottom between the main workbench and the novel laminar flow hood, three storage tanks are arranged on both sides of the novel laminar flow hood, a supporting fixing rod is arranged on the fixed square tube, a first novel stirring mechanism and a second novel stirring mechanism are arranged on the supporting fixing rod; the sterilization component, the preheating component and the mixing and stirring component are fixed on the main workbench by a connecting rod; the sterilization component comprises a rotating disc, three novel beakers A are arranged on the rotating disc, the bottom of the novel beaker A is a conical structure, the novel A heating sleeve is arranged on the outside of beaker A, and a small vibrating rod is arranged on the bottom; the preheating assembly comprises a preheating part and a cooling part; the preheating part comprises an outer wall of the beaker, an inner wall of the beaker, an auxiliary cup wall, a closed cooling tube, a circulating cooling tube, a circulating pump connection port, a water pipe connection port, and a cup wall sliding sleeve; the inner side of the outer wall of the beaker is connected to the inner wall of the beaker; a plurality of closed cooling tubes are connected to the lower part of the outer wall of the beaker; cold water is arranged inside the closed cooling tube; the inner wall of the beaker is connected to the circulating cooling tube; the circulating cooling tube has circulating cold water inside; a circulating pump connection port is arranged on the left side of the circulating cooling tube; the left side of the circulating pump connection port passes through the outer wall of the beaker to connect to the circulating pump; a water pipe connection port is arranged on the right side of the circulating cooling tube; the water pipe connection port passes through the outer wall of the beaker The wall is connected to a water pipe, and the outer side of the inner wall of the beaker is provided with a cup wall sleeve, the outer side of the cup wall sleeve is connected to a circulating cooling pipe, the inner side of the cup wall sleeve is connected to an auxiliary cup wall by a circular screw, the auxiliary cup wall slides in the cup wall sleeve, and fastening slide grooves are provided on both sides of the auxiliary cup wall; the cooling part includes a cooling base, a cooling fan is provided on the cooling base, a rotating motor for driving the cooling fan to rotate is provided in the cooling base, a manual switch for controlling the start and stop of the rotating motor is provided on the outer wall of the cooling base, baffles are provided around the top of the cooling base, the baffles form a rectangular frame, a cooling inner frame is included in the rectangular frame, the cooling inner frame is a mesh structure, and the top of the cooling inner frame is provided with an outer edge extending outward for placement; the cooling A plurality of partitions are arranged in the inner frame; the cooling inner frame is made of plastic material; the mixing and stirring assembly comprises a base and a frame, a central motor is installed in the middle of the inner part of the base, and the shaft end of the central motor is connected to the central rotating shaft, a support is fixed to the upper end of the central rotating shaft, and a cushion ball is arranged between the support and the base, unit motors are installed on the left and right sides of the inner part of the support, and the shaft end of the unit motor is connected to the unit rotating shaft, and heat dissipation nets are arranged on the left and right sides of the unit motor, stirring motors are arranged on the upper left and right sides of the frame, and the bottom end of the frame is welded to the upper surface of the base, the shaft end of the stirring motor is connected to the stirring shaft, and a ferrule is arranged on the lower outer side of the stirring shaft;The support platform forms a rotating structure with the base through a central rotating shaft and a central motor, and the unit motors are symmetrical about the symmetry center line of the support platform. A chuck is fixed to the upper end of the unit rotating shaft, and a hydraulic cylinder is arranged at the inner bottom of the chuck, and a hydraulic oil pipe is connected to the port of the hydraulic cylinder on the side away from the vertical center line of the chuck; hydraulic telescopic rods are evenly arranged on the inner wall of the chuck in a ring shape, and the hydraulic telescopic rods are connected to the hydraulic oil pipe, and a rubber pad is bonded to the side of the hydraulic telescopic rod close to the vertical center line of the chuck, and a cup holder is fixed on the upper inner side of the chuck, and a bulge is arranged in the middle of the upper surface of the cup holder The protrusion is a truncated cone structure, and anti-slip pads are evenly arranged around the protrusion; a limiting pressure ring is fixed on the upper surface of the support, and the inner side surface of the limiting pressure ring is in contact with the edge of the upper surface of the chuck, and a fixing rod is fixed on the upper surface of the limiting pressure ring, and two fixing rods are symmetrically arranged about the vertical center line of the chuck; a pressure edge frame is installed above the fixing rod, and the fixing rod and the pressure edge frame are rotatably connected by connecting bolts, an upper pressure block is installed on the side surface of the pressure edge frame close to the vertical center line of the chuck, and a lower pressure block is arranged below the upper pressure block, and the size between the lower pressure block and the pressure edge frame The support platform is equipped with a screw rod on both sides of the upper surface, and a screw sleeve is provided on the upper outer side of the screw rod. The thread size between the inner side of the screw sleeve and the outer side of the screw rod matches, and the outer wall of the screw sleeve is evenly embedded with a telescopic thin plate. The connecting end of the telescopic thin plate and the screw sleeve is a spherical structure, and a fastening screw passes through the telescopic connection of the telescopic thin plate, and a connecting rod is fixed to the inner side of the outer end of the telescopic thin plate, and a measuring rod is embedded in the middle of the telescopic thin plate, and the connecting rod passes through the inside of the end of the measuring rod. One end of the measuring rod A water immersion sensor is installed; a pneumatic folding frame is installed on the outer side of the ferrule, and the pneumatic folding frame is evenly arranged in a ring shape about the central axis of the ferrule, a support plate is installed at the lower end of the pneumatic folding frame, an air pipe runs through the upper outer side of the ferrule, and the air pipe and the pneumatic folding frame are interconnected, the upper end of the air pipe is connected to an air box, and the support plate forms a rotating knot with the ferrule through the pneumatic folding frame; threaded holes are evenly arranged inside the support plate, and a glass rod is installed below the support plate, the upper end of the glass rod is threadedly connected to the threaded hole, and a rubber head is fixed to the bottom end of the glass rod;The cleaning assembly includes a rotating column, a supporting tube movably penetrates the upper side of the rotating column, a supporting column is fixedly sleeved on the upper side of the supporting tube, a rotating motor is fixedly installed on the outer wall of the supporting column, a gear a is fixedly installed on the lower end of the output shaft of the rotating motor, an annular rack is fixedly installed on the upper side of the rotating column, and the gear a is meshed and connected with the annular rack, two vertical grooves are provided on the outer wall of the rotating column, circular grooves are provided on the upper sides of the two vertical grooves, movable balls are movably connected in the two circular grooves, connecting columns are fixedly installed on the lower sides of the two movable balls, cleaning rods are fixedly installed on the lower sides of the two connecting columns, and the two cleaning rods are movably connected to the two vertical grooves respectively, an installation groove is provided on the inner wall of the rotating column, the supporting tube is movably connected to the installation groove, and there is a A trapezoidal ring, sliders are fixedly installed on the left and right sides of the trapezoidal ring, and sliding grooves are provided on the left and right walls of the device groove. The two sliders are movably connected with the two sliding grooves respectively. The trapezoidal ring is movably sleeved on the support tube, and a threaded groove is provided on the outer wall of the support tube, and a thread is provided on the inner wall of the trapezoidal ring. The trapezoidal ring is meshed and connected with the support tube. A linkage plate is provided on the lower side of the trapezoidal ring, and a pressure tension spring is fixedly installed on the lower side of the linkage plate. A support plate is fixedly installed on the lower end of the tension spring, and the support plate is fixedly sleeved on the support tube. A trigger strip is movably penetrated on one wall of the two vertical grooves close to each other, and the side of the two trigger strips close to each other fits with the inclined surface of the trapezoidal ring, and an arc plate is fixedly installed on one end of the two trigger strips away from each other, and the two arc plates fit with the two cleaning rods respectively. The lower side of the trapezoidal ring is provided with an annular groove, and an annular block is movably connected in both annular grooves, and the lower side of the annular block is fixedly connected to the upper side of the linkage plate; The outer wall of the support column is fixedly mounted with a lifting plate, a support strip is movably passed through the lifting plate, and a bottom plate is fixedly mounted at the lower end of the support strip; A vertical bar is fixedly installed on the lower side of the lifting plate, a rubber ring is fixedly installed on the lower end of the vertical bar, and the rubber ring is movably sleeved on the outer side of the rotating column; The rotating column is provided with a linkage groove, in which a bevel gear is movably connected, and the bevel gear is fixedly sleeved on the support tube; The bevel gear is meshed with two gears b, and the upper sides of the two gears b are movably penetrated by inclined rods, and the two inclined rods are fixedly connected to the upper wall of the linkage groove; The upper walls of the two circular grooves are provided with two connecting grooves, the two connecting grooves are connected with the linkage groove, and movable columns are movably connected in the two connecting grooves; The two movable columns are fixedly connected to the two movable balls respectively, and the two movable columns are fixedly sleeved with gears c; A transverse groove is provided on the lower side of the rotating column, and two telescopic tubes are movably connected in the transverse groove; The two telescopic tubes are both fixedly connected to the supporting tube, and water outlet grooves are provided on the upper sides of the two telescopic tubes.

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