Chromosome slide preparation system and slide preparation method

Abstract

The invention provides a chromosome slide preparation system and a slide preparation method, belongs to the technical field of biological detection, and is used for solving the problems that in the existing chromosome slide preparation operation, the requirements for temperature and humidity of an operation environment are high due to a centrifugation step, and in the prior art, the operation steps of centrifugation, slide dropping and overfire, slide drying and the like in the chromosome slide preparation operation are independent. In order to solve the problems that in the prior art, a cell culture box with glass slides is installed on a slide preparation device in a matched mode, a fixing mechanism and a liquid feeding mechanism are additionally installed in a device body, and the fixing mechanism is divided into a push-pull assembly, an oscillation assembly and a lifting assembly, so that the slide preparation efficiency is low; according to the chromosome preparation device, the liquid feeding assembly and the liquid feeding box of the liquid feeding mechanism are matched, so that the steps of hypotonic, fixing and baking in chromosome preparation can be completed in the same equipment, meanwhile, the steps of transferring, centrifuging and the like in the traditional preparation process are avoided, the preparation efficiency is improved, and the working intensity of operators is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a chromosome preparation system and a preparation method. Background Art

[0002] In modern science, biological detection technology has made great progress. However, during the preparation of cytogenetic specimens for the purpose of chromosome karyotype analysis, how to continuously and stably obtain well-dispersed metaphase division images is an important link related to the subsequent chromosome banding quality and analysis accuracy.

[0003] The current technology has high requirements for the temperature and humidity of the environment during chromosome preparation. In actual operation, the cell suspension before drop-slide needs to be transferred to a centrifuge tube for centrifugation. The subsequent slide preparation, uniform over-firing during the drop-slide operation, and subsequent baking are all independent and require walking back and forth between different devices. The steps are cumbersome and affect work efficiency. The instruments used in the experiment have a single function and cannot meet actual work needs. Summary of the invention

[0004] In view of the shortcomings of the prior art mentioned above, the object of the present invention is to provide a chromosome preparation system and preparation method, which are used to solve the problems in the current chromosome preparation operation that the centrifugation step has high requirements on the temperature and humidity of the operating environment, and the centrifugation, dripping and glass slide drying steps in the chromosome preparation operation in the prior art are independent of each other, and it is necessary to walk back and forth between different preparation equipment, resulting in low preparation efficiency.

[0005] To achieve the above-mentioned and other related purposes, the present invention provides a chromosome preparation system, comprising: a cell culture box, The cell culture box is used to culture experimental cells; the bottom plate of the cell culture box is a glass slide, and the top wall of the cell culture box is provided with an operation hole; Device body, The device body comprises a device body, a push-pull assembly, an oscillating assembly, a lifting assembly and a temperature control assembly; the push-pull assembly is slidably connected to the bottom wall of the device body and is used to push out the mounting fixture of the cell culture box; the oscillating assembly is installed on the push-pull assembly and is used to drive the cell culture box to oscillate horizontally; the lifting assembly is installed on the oscillating assembly and the inclination angle of the cell culture box is controlled by the lifting assembly; the temperature control assembly is installed on the oscillating assembly and when the cell culture box is placed on the temperature control assembly, the temperature in the cell culture box is controlled by the temperature control assembly; Liquid supply mechanism, The liquid feeding mechanism includes a liquid feeding assembly and a liquid feeding box; the liquid feeding box is used to store reagents for film making; the liquid feeding assembly is fixedly connected to the inner side of the top wall of the device body and is arranged corresponding to the cell culture box, the liquid feeding assembly is connected to the liquid feeding box, and the liquid feeding assembly is used to complete the liquid feeding operation on the cell culture box.

[0006] Optionally, the push-pull assembly includes a slide rail and a slide plate; There are at least two slide rails arranged in parallel, and the slide rails are fixedly connected to the bottom wall of the device body; the sliding plate is slidably matched with the slide rails; The oscillation assembly includes a servo motor, an annular slide rail and a horizontal oscillation plate; The servo motor is fixedly connected to the top center of the sliding plate, the servo motor is arranged vertically, and the output end is fixedly connected to the center of the horizontal oscillation plate; the annular slide rail is fixedly connected to the top of the sliding plate around the servo motor; the horizontal oscillation plate and the annular slide rail are slidably matched; The lifting assembly includes a lift and a mounting fixture. There are multiple lifts, and the multiple lifts are evenly distributed vertically on the top of the horizontal oscillation plate, and the output end is fixedly connected to the mounting fixture.

[0007] Optionally, the temperature control component includes a temperature control platform and a support member, and there are multiple support members. The bottom ends of the multiple support members are fixedly connected to the horizontal oscillation plate, and the top ends pass through the bottom plate of the mounting fixture and are fixedly connected to the temperature control platform; the temperature control platform is fixedly connected with mounting guide rails on both sides along the length direction.

[0008] Optionally, a vertically arranged elastic support member is fixedly connected to the bottom plate of the mounting fixture, there are multiple elastic support members, and through holes corresponding to the elastic support members are opened on the temperature control table.

[0009] Optionally, there are multiple liquid supply boxes, including a reagent box and a waste liquid box; The liquid feeding assembly includes a liquid feeding box, a delivery pump and a dropper; the liquid feeding box is fixedly connected to the inner side of the top wall of the device body; there are two delivery pumps, and both are fixedly connected to the inside of the liquid feeding box; the dropper is fixedly connected to the bottom end of the liquid feeding box; Optionally, the dropper includes a liquid feeding dropper and a liquid suction dropper; The tail end of the liquid feeding dropper is connected with a liquid feeding tube, which is connected with a delivery pump and a reagent box in sequence; the tail end of the liquid suction dropper is connected with a liquid discharge tube, which is connected with another delivery pump and a waste liquid tank in sequence.

[0010] Optionally, the liquid feeding component further comprises a liquid suction lifting member, which is vertically fixedly connected to the bottom end of the liquid feeding box, and the output end is fixedly connected to the liquid suction dropper.

[0011] Optionally, a side wall of the cell culture box is fixedly connected with a clamping assembly, and the clamping assembly includes a slide hook and a slide clamping plate; The slide hook is fixedly connected to the bottom of the side wall of the cell culture box body; The glass slide clamping plate is fixedly connected to the side wall away from the glass slide hook, and the glass slide clamping plate is a vertically arranged triangular plate; the vertical side of the glass slide clamping plate is fixedly connected to the side wall of the box body; the end of the bottom side of the glass slide clamping plate away from the side wall of the box body is away from the glass slide and forms a V-shaped gap with the glass slide; a clamping piece is fixedly connected to the long side of the glass slide clamping plate, and the clamping piece is a V-shaped clamping piece, including two supporting columns; a trigger is provided on the supporting column fixedly connected to the glass slide clamping plate, and the other supporting column is vertically arranged and fixedly connected to a hook at the bottom.

[0012] The present invention also provides a chromosome preparation method, comprising the following steps: S1. Take out the cell culture box from the clean bench and mark it; S2. Inoculate the centrifuged specimen into a cell culture box using a pipette, add liquid culture medium, place in an incubator and set up culture environment conditions to form a cell clone group; S3. After forming cell clones, digest the adherent cells with trypsin, replace with new culture medium and wait for them to reattach and grow. The growing cells will grow evenly. S4, installing the cell culture box that has completed adherent growth in the installation fixture in the main body of the device, opening the lifting assembly, lifting one side of the cell culture box through the elastic support, and at the same time sucking out the supernatant in the cell culture box through the delivery pump connected to the liquid pipette; S5. Add hypotonic solution, pre-fixing solution and fixing solution into different reagent boxes respectively, open the liquid supply pipe of the hypotonic solution reagent box, and inject the hypotonic solution into the cell culture box through the delivery pump connected to the liquid supply dropper (turn on the shaking component to ensure that the hypotonic solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 30 minutes; S6, open the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the hypotonic solution through the delivery pump connected to the liquid dropper, open the liquid feeding pipe of the pre-fixative solution reagent box, and inject the pre-fixative solution into the cell culture box through the delivery pump connected to the liquid feeding pipette (turn on the shaking assembly to ensure that the pre-fixative solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 7 minutes; S7, turn on the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the pre-fixative solution through the delivery pump connected to the liquid dropper, open the liquid supply pipe of the fixative reagent box, and inject the fixative solution into the cell culture box through the delivery pump connected to the liquid supply pipette (turn on the shaking assembly to ensure that the fixative solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 5 minutes; S8, repeat step S3 to complete the fixation three times; S9. Turn on the lifting assembly, lift up one side of the cell culture box through the elastic support, and at the same time suck out the fixative through the delivery pump connected to the liquid pipette, turn on the temperature control table, and complete the annealing and baking.

[0013] Optionally, the hypotonic solution is potassium chloride-trisodium citrate dihydrate solution, the pre-fixative solution is glacial acetic acid, and the fixative solution is methanol-glacial acetic acid solution.

[0014] As described above, the chromosome preparation system and preparation method of the present invention have at least the following beneficial effects: The present invention provides a slide making device body that cooperates with a cell culture box installed with a glass slide, and further installs a push-pull component, an oscillation component, a lifting component and a temperature control component in the device body. The cell culture box that completes cell culture is installed in a mounting fixture in a fixing mechanism through the push-pull component, and the liquid feeding mechanism cooperates with the oscillation component, the lifting component and the temperature control component to achieve that the steps of hypotonicity, fixation and baking in chromosome slide making can be completed in the same device, while avoiding the steps of transfer, centrifugation and the like in the traditional slide making process, thereby increasing the slide making efficiency and reducing the workload of operators.

[0015] The present invention facilitates the installation and fixation of the cell culture box by adding a push-pull component, and can drive the cell culture box to oscillate in the horizontal direction by adding an oscillation component to ensure that the hypotonic liquid and the fixative liquid are evenly distributed on the bottom wall of the cell culture box. The waste liquid of the cell culture box can be sucked out by adding a lifting component, cooperating with an elastic support member, and a liquid suction dropper.

[0016] The present invention can ensure uniform baking of the slices by installing a temperature control table, and the installation rails on both sides of the temperature control table facilitate the installation and fixation of the cell culture box.

[0017] The present invention designs a cell culture box as a cell culture box, replaces the bottom plate of the box body with a glass slide, and the glass slide is fixed to the bottom of the box body by clamping with a clamping component installed on the side wall of the box body. The clamping component is designed to be a glass slide hook and a glass slide clamping plate, and a clamping component is added to the glass slide clamping plate. The glass slide is fixed and removed by a trigger and a hook on the clamping component, thereby avoiding the situation in which a scraper needs to be extended from the bottle mouth into the culture bottle during the harvesting process of a traditional cell culture bottle, which is inconvenient to operate and has a low cell harvesting rate.

[0018] The invention provides an operation hole on the top wall of the box body and cooperates with a heat-sealed transparent film, so that the experimenter can adjust the sealing state of the operation hole through the transparent film to perform the experiment operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the overall structure of the present invention; Figure 3 It is a schematic diagram of the overall structure of the present invention; Figure 4 It is a schematic diagram of the coordination between the installation fixture and the lifting assembly of the present invention; Figure 5 It is a schematic diagram of the cooperation between the push-pull component and the oscillating component of the present invention; Figure 6 It is a schematic structural diagram of the liquid feeding assembly of the present invention; Figure 7 It is a schematic diagram of the overall structure of the cell culture box of the present invention; Figure 8 It is a schematic diagram of the overall structure of the cell culture box of the present invention.

[0020] Component number description Device body-1, push-pull component-2, sliding plate-201, oscillation component-3, horizontal oscillation plate-301, temperature control table-4, mounting rail-401, mounting fixture-5, liquid supply component-6, liquid suction lifting component-601, liquid supply box-7, cell culture box-8, slide glass-9, slide hook-10, slide clamping plate-11, operation hole-12. DETAILED DESCRIPTION

[0021] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0022] See also Figures 1 to 8 . It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical content disclosed by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0023] The following embodiments are only for illustration purposes and can be combined with each other, and are not limited to the contents presented in the following single embodiments.

[0024] See also Figures 1 to 8The present invention provides a chromosome film making system, comprising a film making device, a liquid feeding mechanism and a cell culture box 8; the film making device comprises a device body 1, the device body 1 is a mounting box, and a mounting fixture 5 of the cell culture box 8 is arranged inside, and the film making device also comprises a push-pull component 2, an oscillation component 3, a lifting component and a temperature control component; the push-pull component 2 is slidably connected to the bottom wall of the device body 1, and is used to push out the mounting fixture 5 of the cell culture box 8; the oscillation component 3 is installed on the top of the push-pull component 2, and is used to drive the cell culture box 8 to oscillate horizontally; the lifting component is installed on the top of the oscillation component 3, and the output end is fixedly connected to the mounting fixture 5; the temperature control component is fixedly installed on the oscillation component 3, and when the cell culture box 8 is placed on the temperature control component, the temperature in the cell culture box 8 is controlled by the temperature control component; the liquid feeding mechanism comprises a liquid feeding component 6 and a liquid feeding box 7; the liquid feeding box 7 is fixedly connected to the bottom wall of the device body 1 The outer side is used to store reagents for preparation; the liquid feeding component 6 is fixedly connected to the inner side of the top wall of the device body 1 and is arranged corresponding to the cell culture box 8. The liquid feeding component 6 is connected to the liquid feeding box 7, and the liquid feeding component 6 is used to complete the liquid feeding operation of the cell culture box 8; the bottom plate of the cell culture box 8 is a glass slide 9, and the top wall of the cell culture box 8 is provided with an operation hole 12. In the present application, a chromosome device body 1 is matched with a cell culture box 8 equipped with a glass slide 9, a fixing mechanism and a liquid feeding mechanism are added to the device body 1, and the fixing mechanism is split into a push-pull component 2, an oscillation component 3 and a lifting component. The cell culture box 8 that completes cell culture is installed in the installation fixture 5 in the fixing mechanism through the push-pull component 2. The liquid feeding mechanism is matched with the oscillation component 3 and the lifting component to achieve the steps of hypotonicity, fixation and baking in chromosome preparation. It can be completed in the same device, while avoiding the steps of transfer, centrifugation and the like in the traditional preparation process, thereby increasing the preparation efficiency and reducing the workload of the operator.

[0025] In this embodiment, please refer to Figure 1 and Figure 5The push-pull component 2 includes a slide rail and a slide plate 201; there are at least two slide rails arranged in parallel, and the slide rails are fixedly connected to the top of the bottom wall of the device body 1; the slide plate 201 is slidably matched with the slide rail (the side walls of the device body 1 parallel to the slide rails are provided with guide grooves, and the guide grooves are used in conjunction with the slide plate 201; the side wall of the device body 1 perpendicular to the slide rail is hinged to the device body 1); the oscillation component 3 includes a servo motor, an annular slide rail and a horizontal oscillation plate 301; the servo motor is fixedly connected to the top center of the slide plate 201, the servo motor is arranged vertically, and the output end is fixedly connected to the center of the horizontal oscillation plate 301; the annular slide rail is fixedly connected to the top of the slide plate 201 around the servo motor; the horizontal oscillation plate 301 Sliding with the annular slide rail; the lifting component includes an elevator and a mounting fixture 5, and the elevator is multiple, and the multiple elevators are vertically evenly distributed on the top of the horizontal oscillation plate 301, and the output end is fixedly connected to the mounting fixture 5. The application ensures that the sliding plate 201 can slide horizontally smoothly on the bottom plate of the device body 1 through the installation of multiple strip slide rails and the opening of guide grooves. The hinged side walls facilitate the opening and closing of the device body 1 and the pulling out of the sliding plate 201. The horizontal oscillation of the horizontal oscillation plate 301 is ensured by the installation of annular slide rails and servo motors. The servo motor switches between forward and reverse rotation to drive the horizontal oscillation plate 301 to oscillate and rotate clockwise and counterclockwise, ensuring that the hypotonic solution and fixative solution are evenly distributed on the bottom wall of the cell culture box 8 during chromosome preparation.

[0026] In this embodiment, please refer to Figure 1 and Figure 4 The temperature control component includes a temperature control table 4 and a support member, and the support member is multiple. The bottom ends of the multiple support members are fixedly connected to the horizontal oscillation plate 301, and the top ends penetrate the bottom plate of the mounting fixture 5 and are fixedly connected to the temperature control table 4; the temperature control table 4 is fixedly connected with mounting rails 401 on both sides along the length direction. The present application facilitates the sequential installation of the cell culture box 8 by adding mounting rails 401 on both sides of the temperature control table 4. The two mounting rails 401 are arranged in parallel along the length direction. The installation of the temperature control table 4 realizes the step of baking the chromosomes. The installation of the temperature control table 4 also avoids the situation where the cell membrane is ruptured and the chromosomes are released due to the unstable external environment, ensuring uniform baking.

[0027] In this embodiment, please refer to Figure 1 , Figure 3 , Figure 4 and Figure 6, a vertically arranged elastic support is fixedly connected to the bottom plate of the mounting fixture 5, there are multiple elastic support members, through holes corresponding to the elastic support members are opened on the temperature control table 4, there are multiple liquid feeding boxes 7, including a reagent box and a waste liquid box; the liquid feeding assembly 6 includes a liquid feeding box, a delivery pump and a dropper; the liquid feeding box is fixedly connected to the inner side of the top wall of the device body 1; there are two delivery pumps, and both are fixedly connected to the inside of the liquid feeding box; the dropper is fixedly connected to the bottom end of the liquid feeding box; the dropper includes a liquid feeding dropper and a liquid suction dropper; the tail end of the liquid feeding dropper is connected to a liquid feeding tube, and the liquid feeding tube is connected to a delivery pump, a reagent box, and a reagent tank in turn. The tail end of the liquid-sucking dropper is connected with a liquid discharge pipe, which is connected with another delivery pump and a waste liquid tank in turn. The liquid-feeding assembly 6 also includes a liquid-sucking lifting member 601, which is vertically fixedly connected to the bottom end of the liquid-feeding box, and the output end is fixedly connected to the liquid-sucking dropper. The present application installs an elastic support member on the bottom plate of the mounting fixture 5, and installs a liquid-sucking lifting member 601 on the liquid-sucking dropper. When absorbing waste liquid, the mounting fixture 5 is driven to rise by the lifting assembly until the cell culture box 8 is tilted under the upward force of the elastic support member, and the descending liquid-sucking dropper cooperates with the tilted cell culture box 8 to complete the absorption of waste liquid.

[0028] In this embodiment, please refer to Figure 7 and Figure 8The side wall of the cell culture box 8 is fixedly connected with a clamping assembly, and the clamping assembly includes a glass slide hook 10 and a glass slide clamping plate 11; the glass slide hook 10 is fixedly connected to the bottom of the side wall of the cell culture box body; the glass slide clamping plate 11 is fixedly connected to the side wall away from the glass slide hook 10, and the glass slide clamping plate 11 is a vertically arranged triangular plate; the vertical side of the glass slide clamping plate 11 is fixedly connected to the side wall of the box body; the end of the bottom edge of the glass slide clamping plate 11 away from the side wall of the box body is away from the slide 9, and forms a V-shaped gap with the slide 9 (for easy installation and removal of the slide 9); a clamping piece is fixedly connected to the long side of the slide clamping plate 11, and the clamping piece is a V-shaped clamping piece, including two support columns; a trigger is provided on the support column fixedly connected to the slide clamping plate 11, and the other support column is vertically connected to the slide 9. A hook is arranged in the middle and fixedly connected at the bottom, the glass slide 9 includes a glass slide frosting area and a glass slide culture area; the glass slide culture area is adapted to the shape of the box body and cooperates with the glass slide hook 10 on the side wall of the box body; the end of the glass slide frosting area away from the glass slide culture area cooperates with the hook. In the present application, the cell culture dish is designed into a cell culture box 8, and the bottom plate of the box body is replaced with a glass slide 9, the glass slide 9 is fixed to the bottom of the box body by a clamping component installed on the side wall of the box body, and the clamping component is designed into a glass slide hook 10 and a glass slide clamping plate 11, and a clamping part is added to the glass slide clamping plate 11. The glass slide 9 is fixed and disassembled by the trigger on the clamping part and the hook, thereby avoiding the need for a scraper to extend from the bottle mouth into the culture bottle during the harvesting process of the traditional cell culture bottle, which is inconvenient to operate and has a low cell harvesting rate.

[0029] The present invention also provides a chromosome preparation method, comprising the following steps: S1. Take out the cell culture box 8 from the clean bench and mark it; S2, inoculating the centrifuged specimen into the cell culture box 8 through the operation hole 12 with a pipette, adding liquid culture medium, placing it in an incubator and setting the culture environment conditions to form a cell clone group; S3. After forming cell clones, digest the adherent cells with trypsin, replace with new culture medium and wait for them to reattach and grow. The growing cells will grow evenly. S4, installing the cell culture box 8 that has completed the adherent growth in the installation fixture 5 in the device body 1, opening the lifting assembly, lifting one side of the cell culture box 8 through the elastic support, and at the same time sucking out the supernatant in the cell culture box 8 through the delivery pump connected to the liquid pipette; S5, respectively inject the hypotonic solution, the pre-fixing solution and the fixing solution into different reagent boxes, open the liquid feeding pipe of the hypotonic solution reagent box, and inject the hypotonic solution into the cell culture box 8 through the delivery pump connected to the liquid feeding dropper (turn on the shaking component 3 to ensure that the hypotonic solution is evenly distributed on the bottom wall of the cell culture box 8), and let it stand for 30 minutes; S6, open the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the hypotonic solution through the delivery pump connected to the liquid dropper, open the liquid feeding pipe of the pre-fixative solution reagent box, and inject the pre-fixative solution into the cell culture box 8 through the delivery pump connected to the liquid feeding pipette (open the shaking assembly 3 to ensure that the pre-fixative solution is evenly distributed on the bottom wall of the cell culture box 8), and let it stand for 7 minutes; S7, open the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the pre-fixative solution through the delivery pump connected to the liquid dropper, open the liquid feeding pipe of the fixative reagent box, and inject the fixative solution into the cell culture box 8 through the delivery pump connected to the liquid feeding pipette (open the shaking assembly 3 to ensure that the fixative solution is evenly distributed on the bottom wall of the cell culture box 8), and let it stand for 5 minutes; S8, repeat step S3 to complete the fixation three times; S9, open the lifting assembly, lift up one side of the cell culture box through the elastic support, and at the same time suck out the fixative through the delivery pump connected to the liquid suction dropper, turn on the temperature control table 4, and complete the annealing and baking.

[0030] In this embodiment, the hypotonic solution is potassium chloride-trisodium citrate dihydrate solution, the pre-fixative solution is glacial acetic acid, and the fixative solution is methanol-glacial acetic acid solution.

[0031] In the present invention, steps S4-S9 are controlled by a fixed program, and the film-making equipment operates according to the fixed program to automatically complete the film-making work.

[0032] In summary, the present invention overcomes various shortcomings in the prior art.

[0033] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A chromosome preparation system, characterized in that: include: Cell culture box, The cell culture box is used to culture experimental cells; the bottom plate of the cell culture box is a glass slide, and the top wall of the cell culture box is provided with an operation hole; Device body, The device body comprises a device body, a push-pull assembly, an oscillating assembly, a lifting assembly and a temperature control assembly; the push-pull assembly is slidably connected to the bottom wall of the device body, and is used to push out the mounting fixture of the cell culture box; the oscillating assembly is installed on the push-pull assembly, and is used to drive the cell culture box to oscillate horizontally; the lifting assembly is installed on the oscillating assembly, and the inclination angle of the cell culture box is controlled by the lifting assembly; the temperature control assembly is installed on the oscillating assembly, and when the cell culture box is placed on the temperature control assembly, the temperature in the cell culture box is controlled by the temperature control assembly; Liquid supply mechanism, The liquid feeding mechanism includes a liquid feeding assembly and a liquid feeding box; the liquid feeding box is used to store reagents for film making; the liquid feeding assembly is fixedly connected to the inner side of the top wall of the device body and is arranged corresponding to the cell culture box, the liquid feeding assembly is connected to the liquid feeding box, and the liquid feeding assembly is used to complete the liquid feeding operation on the cell culture box.

2. The chromosome preparation system according to claim 1, characterized in that: The push-pull assembly includes a slide rail and a slide plate; There are at least two slide rails arranged in parallel, and the slide rails are fixedly connected to the bottom wall of the device body; the sliding plate is slidably matched with the slide rails; The oscillation assembly includes a servo motor, an annular slide rail and a horizontal oscillation plate; The servo motor is fixedly connected to the center of the top of the sliding plate. The servo motor is arranged vertically, and the output end is fixedly connected to the center of the horizontal oscillation plate. The annular slide rail is fixedly connected to the top of the sliding plate around the servo motor. The horizontal oscillation plate and the annular slide rail are slidably matched. The lifting assembly includes a lift and a mounting fixture. There are multiple lifts, and the multiple lifts are evenly distributed vertically on the top of the horizontal oscillation plate, and the output end is fixedly connected to the mounting fixture.

3. The chromosome preparation system according to claim 2, characterized in that: The temperature control component includes a temperature control platform and a support member. There are multiple support members, the bottom ends of which are fixedly connected to the horizontal oscillation plate, and the top ends penetrate the bottom plate of the mounting fixture and are fixedly connected to the temperature control platform; the temperature control platform is fixedly connected to mounting guide rails on both sides along the length direction.

4. The chromosome preparation system according to claim 3, characterized in that: A vertically arranged elastic support member is fixedly connected to the bottom plate of the mounting fixture, there are multiple elastic support members, and through holes corresponding to the elastic support members are opened on the temperature control table.

5. The chromosome preparation system according to claim 1, characterized in that: There are multiple liquid supply boxes, including a reagent box and a waste liquid box; The liquid feeding assembly includes a liquid feeding box, a delivery pump and a dropper; the liquid feeding box is fixedly connected to the inner side of the top wall of the device body; there are two delivery pumps, both of which are fixedly connected to the inside of the liquid feeding box; the dropper is fixedly connected to the bottom end of the liquid feeding box.

6. The chromosome preparation system according to claim 5, characterized in that: The dropper comprises a liquid supply dropper and a liquid suction dropper; The tail end of the liquid feeding dropper is connected with a liquid feeding tube, which is connected with a delivery pump and a reagent box in sequence; the tail end of the liquid suction dropper is connected with a liquid discharge tube, which is connected with another delivery pump and a waste liquid tank in sequence.

7. The chromosome preparation system according to claim 6, characterized in that: The liquid feeding component also includes a liquid suction lifting member, which is vertically fixedly connected to the bottom end of the liquid feeding box, and the output end is fixedly connected to the liquid suction dropper.

8. The chromosome preparation system according to claim 1, characterized in that: The side wall of the cell culture box is fixedly connected with a clamping assembly, and the clamping assembly includes a slide hook and a slide clamping plate; The slide hook is fixedly connected to the bottom of the side wall of the cell culture box body; The glass slide clamping plate is fixedly connected to the side wall away from the glass slide hook, and the glass slide clamping plate is a vertically arranged triangular plate; the vertical side of the glass slide clamping plate is fixedly connected to the side wall of the box body; the end of the bottom side of the glass slide clamping plate away from the side wall of the box body is away from the glass slide and forms a V-shaped gap with the glass slide; a clamping piece is fixedly connected to the long side of the glass slide clamping plate, and the clamping piece is a V-shaped clamping piece, including two supporting columns; a trigger is provided on the supporting column fixedly connected to the glass slide clamping plate, and the other supporting column is vertically arranged and fixedly connected to a hook at the bottom.

9. A method for preparing chromosome slices, characterized in that: The steps include: S1. Take out the cell culture box from the clean bench and mark it; S2. Inoculate the centrifuged specimen into a cell culture box using a pipette, add liquid culture medium, place in an incubator and set up culture environment conditions to form a cell clone group; S3. After forming cell clones, digest the adherent cells with trypsin, replace with new culture medium and wait for them to reattach and grow. The growing cells will grow evenly. S4, installing the cell culture box that has completed adherent growth in the installation fixture in the main body of the device, opening the lifting assembly, lifting one side of the cell culture box through the elastic support, and at the same time sucking out the supernatant in the cell culture box through the delivery pump connected to the liquid pipette; S5. Add hypotonic solution, pre-fixing solution and fixing solution into different reagent boxes respectively, open the liquid supply pipe of the hypotonic solution reagent box, and inject the hypotonic solution into the cell culture box through the delivery pump connected to the liquid supply dropper (turn on the shaking component to ensure that the hypotonic solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 30 minutes; S6, open the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the hypotonic solution through the delivery pump connected to the liquid dropper, open the liquid feeding pipe of the pre-fixative solution reagent box, and inject the pre-fixative solution into the cell culture box through the delivery pump connected to the liquid feeding pipette (turn on the shaking assembly to ensure that the pre-fixative solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 7 minutes; S7, turn on the lifting assembly, lift one side of the cell culture box through the elastic support, and at the same time suck out the pre-fixative solution through the delivery pump connected to the liquid dropper, open the liquid supply pipe of the fixative reagent box, and inject the fixative solution into the cell culture box through the delivery pump connected to the liquid supply pipette (turn on the shaking assembly to ensure that the fixative solution is evenly distributed on the bottom wall of the cell culture box), and let it stand for 5 minutes; S8, repeat step S3 to complete the fixation three times; S9. Turn on the lifting assembly, lift up one side of the cell culture box through the elastic support, and at the same time suck out the fixative through the delivery pump connected to the liquid pipette, turn on the temperature control table, and complete the annealing and baking.

10. The chromosome preparation method according to claim 9, characterized in that: The hypotonic solution is potassium chloride-trisodium citrate dihydrate solution, the pre-fixative solution is glacial acetic acid, and the fixative solution is methanol-glacial acetic acid solution.

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