Vibration signal synthesis method for interfering mating behavior of liriomyza trifoliate

Abstract

The invention discloses a vibration signal synthesis method for interfering the mating behavior of Liriomyza trifoliate, which can effectively trigger resonance on leaves and interfere natural vibration signals of Liriomyza trifoliate by playing artificially synthesized vibration signals with frequencies similar to those of Liriomyza trifoliate courtship signals, so that the communication between male and female is submerged, and the mating behavior of Liriomyza trifoliate is inhibited. The method has the advantages of preventing the pests from smoothly completing the mating process, utilizing the synthesis and transmission technology of the vibration signals, having remarkable advantages, not only effectively reducing the population reproductive capacity of the pests, but also avoiding the problems of environmental pollution and pest resistance of insecticides, and being a sustainable solution for agricultural pest prevention and control in the future.

Description

Technical Field

[0001] The invention relates to the technical field of pest control methods, and in particular to a vibration signal synthesis method for interfering with the mating behavior of leafminer. Background Art

[0002] In insects, courtship behavior is usually accomplished through chemical, visual and mechanical signals to coordinate and promote complex courtship interactions; current integrated pest management strategies often rely on chemical signal interference techniques, such as pheromone interference, to disrupt insect mating patterns; however, so far, research has shown that the three-leaf leafminer does not rely on sex pheromones for mating, but instead communicates through vibration signals, which makes traditional pheromone interference strategies of limited effectiveness in the control of this pest.

[0003] Mechanical signal interference is an emerging biological control strategy, especially for pests that rely on vibration signals for courtship. Mechanical signals can generally be divided into two categories: vibrations (sounds) transmitted through the air and vibrations transmitted through solid substrates. The courtship behavior of the three-leaf leafminer mainly relies on the substrate vibration signals transmitted through the leaves. The vibration signal synthesis and interference technology for the three-leaf leafminer will provide an environmentally friendly and efficient option for pest control of crops.

[0004] Although vibration communication is a very common signal transmission method in insects, there are relatively few in-depth studies on it. Recent studies have shown that the mechanical vibration signals of insects can be interfered with by external stimuli. For example, by playing specific vibration signals, the insect duet signals can be interrupted, thereby interrupting their mating behavior. However, the current research on the mechanism of courtship signal generation of Flyminer trifoliata is still insufficient, especially in the understanding of its signal characteristics and generation methods. Summary of the invention

[0005] The main purpose of the invention is to provide a vibration signal synthesis method for interfering with the mating behavior of Liriomyza trifoliata.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] A vibration signal synthesis method for interfering with the mating behavior of leafminer, specifically comprising the following steps:

[0008] Step 1: Use a laser vibrometer and a high-speed camera to obtain the natural vibration signal of the three-leaf leafminer in the courtship behavior, analyze the natural vibration signal to obtain the signal characteristics, and determine the main parameters of the signal characteristics; the courtship behavior of the three-leaf leafminer is transmitted by inducing leaf surface vibration. The specific process of the courtship behavior is recorded by a high-speed camera at a rate of 60 frames per second. The image of each experiment is taken with an exposure time of 5.13ms and a resolution of 1920x1080 pixels. The video is directly stored on the computer for frame-by-frame analysis; Use a laser vibrometer to naturally record the vibration signal, aim the laser beam at the position of the reflective membrane close to the leafminer, record the vibration speed at the laser, and import the digital signal into Adobe Audition CS6 software through a USB digital audio interface for sampling. The sampling rate is 48kHz, the resolution is 16 bits, and the data is saved as a wav file. Use a high-speed camera to synchronously record the behavior of the three-leaf leafminer in order to link the vibration signal with the specific behavior.

[0009] Step 2: Using signal generation software, an artificial synthetic vibration signal is designed based on the natural vibration signal;

[0010] Step 3: A high-quality loudspeaker is used to transmit the artificially synthesized vibration signal through the air and induce blade resonance; a non-contact transmission method is adopted to ensure the wide applicability of the interference signal and will not cause damage to the environment and crops, ensuring the wide applicability of the interference signal and will not cause damage to the environment and crops.

[0011] The vibration caused by the artificial synthetic vibration signal on the blade surface is detected by a laser vibrometer, and the parameters are adjusted and optimized to improve the interference effect;

[0012] Step 4: Place male and female adults of the three-leaf leafminer on the disturbed leaves, play artificial synthetic vibration signals, and record the courtship and mating behaviors of the three-leaf leafminer. Based on the results, further optimize the signal parameters.

[0013] In order to improve the accuracy of experimental test results, experimental records were performed multiple times.

[0014] Furthermore, the main parameters of the natural vibration signal include frequency, amplitude, duration and signal interval; according to the natural courtship signal characteristics of the three-leaf leafminer, the interference signal is designed with a specific frequency, amplitude, duration and signal interval to improve the interference effect.

[0015] Furthermore, the frequency is mainly concentrated in 250-450 Hz, the amplitude range is 20-40 μm, the duration is 0.4-0.6 s, and the signal interval is 0.3-0.5 s.

[0016] Furthermore, the frequency of the artificially synthesized vibration signal is greater than or equal to the frequency of the natural signal to enhance the interference effect. The amplitude of the artificially synthesized vibration signal is slightly higher than the amplitude of the natural signal to ensure that it can still maintain effective strength when propagating in the air. The time characteristics of the artificially synthesized vibration signal are similar to the time characteristics of the natural signal.

[0017] Furthermore, the frequency of the artificial synthetic vibration signal is set at 300-500 Hz, the amplitude range of the artificial synthetic vibration signal is 20-40 μm, the duration of the artificial synthetic vibration signal is 0.4-0.6 s, and the signal interval is 0.3-0.5 s.

[0018] Furthermore, the speaker is set at a distance of 5-10 cm from the target blade, and the volume of the speaker is moderate, which can cover the leaves in the entire experimental area and avoid interference from environmental noise. The playback can be continuous or random to further simulate the unpredictability of natural signals.

[0019] Furthermore, the relevant experiments on the three-leaf leafminer were carried out on a shock-proof table in a soundproof room. A layer of 400-mesh nylon net was laid on the shock-proof table to minimize signal distortion and enhance the amplitude of the vibration signal. A pair of three-leaf leafminers were gently transferred to the nylon net and covered with a plastic culture dish to prevent them from escaping, and the natural vibration signal was recorded with a laser vibrometer.

[0020] Furthermore, the leaves are kidney bean leaves, and the end of the petiole is inserted into a plastic bottle containing 100 ml of water to ensure the freshness of the leaves.

[0021] Furthermore, the courtship and mating behaviors of the leafminer include the duration of courtship behavior, the frequency changes of duet interactions, and the mating success rate.

[0022] Furthermore, when the laser vibrometer records the vibration signal, it only analyzes the vibration signal within 2 cm of the laser point.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] Compared with traditional chemical insecticide methods, the present invention has many advantages. First, the use of artificially synthesized vibration signals for interference is a completely environmentally friendly biological control strategy that will not have any negative impact on the environment and non-target organisms. Secondly, this method interferes with the courtship behavior of the three-leaf leafminer, controls its population reproduction from the source, and avoids the problem of pests developing resistance to pesticides. Finally, airborne signal technology is easy to apply on a large scale in the field and has a high promotion value. Combined with the existing integrated agricultural pest management system, this technology can complement other biological control methods, further improve the efficiency of prevention and control, reduce the use of pesticides, and ensure the yield and quality of crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic flow chart of a method for synthesizing vibration signals for interfering with the courtship behavior of Liriomyza trifoliata;

[0026] Figure 2 This is a diagram of the application of artificial synthetic vibration signals to interfere with the mating behavior of Liriomyza trifoliata. DETAILED DESCRIPTION

[0027] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the protection scope of the present invention.

[0028] Example

[0029] See also Figure 1 As shown, for the collection and analysis of natural vibration signals, the petiole end of the bean leaf was inserted into a plastic bottle containing 100 ml of water, a cylindrical plastic chamber with a diameter of 6 cm and a height of 2 mm was placed above the leaf and covered with a transparent glass sheet, and a pair of three-leaf leafminers lightly anesthetized by carbon dioxide were gently transferred to the chamber, and the courtship behavior was recorded at a rate of 60 frames per second with a high-speed camera. The images of each experiment were taken with an exposure time of 5.13 ms and a resolution of 1920x1080 pixels. The videos were directly stored on the computer for frame-by-frame analysis. A total of 41 pairs of three-leaf leafminers were tested in the experiment, and the recording time for each pair was 30 minutes, or until the end of mating.

[0030] A layer of 400-mesh nylon net was laid on the shock-proof table in the soundproof room. A pair of three-leaf leafminers were gently transferred to the nylon net and covered with a plastic culture dish to prevent them from escaping. A laser vibrometer was used to record the vibration signals generated by the courtship behavior of the three-leaf leafminers on the leaves. Only the vibration signals within 2 cm of the laser point were analyzed. Finally, vibration signal data of 26 pairs of three-leaf leafminers were collected.

[0031] The vibration signal data were analyzed to obtain the signal characteristics of the natural vibration signal generated by the courtship behavior of the three-leaved leafminer. It was determined that the frequency range of the natural vibration signal was 250-450 Hz, the amplitude was 20-40 μm, the duration was 0.4-0.6 s, and the signal interval was 0.3-0.5 s.

[0032] Design an artificial synthetic vibration signal. According to the signal characteristics of the natural vibration signal, use signal generation software such as MATLAB or Audacity to design the interference signal. Determine that the frequency range of the artificial synthetic vibration signal is 300-500 Hz, the amplitude is 20-40 μm, the duration is 0.4-0.6 s, and the signal interval is 0.3-0.5 s.

[0033] The signal transmission device is selected by installing a high-quality audio speaker 5-10 cm away from the target blade. The synthesized artificial vibration signal is transmitted through the air through the high-quality speaker to cause the blade to vibrate. The volume of the speaker and the frequency of the signal can be adjusted according to the actual situation.

[0034] Signal testing and adjustment, using a laser vibrometer to detect the vibration caused by the audio signal on the blade surface.

[0035] Experimental verification and optimization: male and female adults of Liriomyza trifoliata were placed on disturbed leaves, synthetic interference signals were played, and their courtship and mating behaviors were recorded. Based on the results, the main parameters of the interference signals were adjusted and optimized. Each experiment lasted 30 minutes. Figure 2 As shown, the mating behavior of the leafminer in the embodiment was significantly disturbed.

[0036] Comparative Example

[0037] The difference between the comparative example and the embodiment is that the comparative example does not play any interference signal.

[0038] The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiment. Any equivalent modifications or changes made by ordinary technicians in this field based on the contents disclosed by the present invention should be included in the protection scope recorded in the claims.

Claims

1. A vibration signal synthesis method for interfering with the mating behavior of Liriomyza trifoliata, characterized in that: The specific steps include: Step 1: using a laser vibrometer and a high-speed camera to obtain the natural vibration signal of the leafminer during the courtship behavior, analyzing the natural vibration signal to obtain the signal characteristics, and determining the main parameters of the signal characteristics; Step 2: Using signal generation software, an artificial synthetic vibration signal is designed based on the natural vibration signal; Step 3: A high-quality speaker is used to transmit the artificial synthetic vibration signal through the air and induce the blade to resonate; a laser vibrometer is used to detect the vibration caused by the artificial synthetic vibration signal on the blade surface, and the parameters are adjusted and optimized; Step 4: Place male and female adults of the three-leaf leafminer on the disturbed leaves, play artificial synthetic vibration signals, and record the courtship and mating behaviors of the three-leaf leafminer. Based on the results, further optimize the signal parameters.

2. The vibration signal synthesis method for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The natural vibration signal is a blade vibration signal caused by the courtship behavior of the leafminer, and the main parameters include frequency, amplitude, duration and signal interval.

3. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 2, characterized in that: The frequency is mainly concentrated in 250-450 Hz, the amplitude range is 20-40 μm, the duration is 0.4-0.6 s, and the signal interval is 0.3-0.5 s.

4. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The main parameters of the artificial synthetic vibration signal include frequency, amplitude, duration and signal interval.

5. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 4, characterized in that: The frequency of the artificial synthetic vibration signal is set at 300-500 Hz, the amplitude range of the artificial synthetic vibration signal is 20-40 μm, the duration of the artificial synthetic vibration signal is 0.4-0.6 s, and the signal interval is 0.3-0.5 s.

6. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The loudspeaker is arranged at a distance of 5-10 cm from the target blade, and the volume of the loudspeaker can cover the blades in the entire environment, and the playback can be continuous or random.

7. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The test of the natural vibration signal of the three-leaf leafminer is carried out on a shockproof table in a soundproof room, on which a layer of 400-mesh nylon net is laid. A pair of three-leaf leafminers is gently transferred to the nylon net, and then covered with a plastic culture dish to prevent them from escaping, and the natural vibration signal is recorded with a laser vibrometer.

8. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The leaves are kidney bean leaves, and the freshness of the leaves can be maintained during the process.

9. The method for synthesizing vibration signals for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: The courtship and mating behaviors of the leafminer include the duration of courtship behaviors, the frequency changes of duet interactions and the mating success rate.

10. The vibration signal synthesis method for interfering with the mating behavior of Liriomyza trifoliata according to claim 1, characterized in that: When the laser vibrometer records the vibration signal, it only analyzes the vibration signal within 2 cm of the laser point.

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