Animal skin burn modeling standardization device

Abstract

The invention relates to the technical field of burn and scald experiments, in particular to an animal skin burn modeling standardization device which mainly structurally comprises a main machine, a handle connected with the main machine and a metal sheet connected with the handle through a metal rod. The main machine is at least provided with a temperature adjusting knob, a switch button, a temperature display screen and a pressure display screen, the handle is at least provided with a pressure adjusting push rod, a heating wire, a heat insulation layer, a pressure sensor and a spring are at least arranged in the handle, the burn degree is controlled by adjusting temperature and pressure and shortening contact time, and I-degree or shallow II-degree burn is achieved. The shape and the size of the metal sheet can be adjusted to carry out burn modeling on animals such as mice or rats, especially to prepare deep II-degree burn models, and the model can also be prepared through measures such as overheat protection and emergency shutdown. The safety of personnel, animals and equipment in the experiment process can be guaranteed, and the device can be widely applied to multiple fields of basic medicine such as preclinical medicine screening, treatment method evaluation and burn pathological mechanism analysis.

Description

Technical field:

[0001] The invention relates to the technical field of burn and scald experiments, and relates to a standardized device for animal skin burn modeling, which can prepare burn models, especially deep II degree burn models, on the skins of various large, medium and small animals. Background technology:

[0002] Traditional treatments such as flap transplantation and burn plasters used in early burn healing can help burn patients recover to a certain extent, but there are problems such as large surgical trauma, long recovery time, and many complications. With the continuous advancement of science and technology, research in the field of burn repair has gradually shifted to a more advanced and refined direction. The emergence of stem cell transplantation technology has brought revolutionary changes to the field of burn repair. In addition to stem cell transplantation technology, the application of new materials such as artificial dermal scaffolds and composite biological dressings has also brought important breakthroughs in the field of burn repair. However, with the gradual deepening of research, the previous mouse burn model is no longer sufficient to support scientific exploration. There is an urgent need to prepare a large animal model that is closer to human burns. The existing technology and tools are far from meeting research needs.

[0003] At present, the establishment of large animal models of burns mainly adopts the methods of flame burns or chemical burns: flame burns are simple to operate and have obvious effects, but it is difficult to control the exact temperature, and improper operation may lead to the death of animals, and there are certain risks for experimenters; chemical burns have higher controllability, but chemical agents are highly corrosive and irritating, which may cause other unknown effects on animals, and also bring certain risks to experimenters. With the continuous deepening of research, the controllability and safety of the burn modeling process are also increasing, and traditional modeling methods can no longer meet the needs of large-scale, high-precision experiments.

[0004] Chinese Patent 202221418172.7 discloses a rat scald wound standardization device, comprising a pressure plate pressed on the surface of rat skin, an opening provided on the pressure plate for the rat skin to be squeezed out after being pressed, a plurality of limiting nails that penetrate into the surface layer of rat skin are provided on the side of the opening close to the rat skin, and the limiting nails are evenly spaced along the outer peripheral edge of the opening, the opening is equipped with an ironing plate for scalding the rat skin, the ironing plate covers the side of the opening away from the rat skin, and a heat insulation layer is provided on the side of the opening away from the rat skin, the inner peripheral edge of the opening has an annular thin plate that abuts against the rat skin along its radial direction, the opening has a conical structure whose radial cross-section gradually narrows from the rat skin to the annular thin plate, a pressure buffer layer is provided between the pressure plate and the rat skin, a buffer layer opening is provided on the pressure buffer layer for the rat skin to squeeze out of the opening, and the buffer layer opening ring is sleeved on the outer periphery of the opening On the peripheral side, the opening of the buffer layer is a conical structure whose cross section gradually narrows from the rat skin to the opening. The pressure buffer layer is a polyurethane foam layer attached to the pressure plate. The side of the polyurethane foam layer close to the rat skin is provided with a friction layer to prevent the rat skin from sliding. The pressure plate and the pressure buffer layer are overall an arc-shaped plate-shaped structure that matches the rat shape. The ironing plate is an arc-shaped plate-shaped structure with the same curvature as the pressure plate. The ironing plate is a heat-conducting copper plate, and a heating coil connected to the power supply mechanism is inserted into the heat-conducting copper plate. The side of the limiting pin close to the opening is a planar structure arranged parallel to the insertion direction, and the radial cross-section of the side of the limiting pin far from the opening is a conical knot that gradually narrows from the opening to the rat skin. The scald model is prepared on the rat skin through the cooperation of the pressure plate and the ironing plate, which is only suitable for the preparation of models of small animals such as mice, and can only control the scald area, but cannot control the temperature and degree of the scald.

[0005] Therefore, there is an urgent need for a more mature and stable large animal burn modeling instrument to prepare burn model-assisted experiments. The small animal burn modeling instrument that can control temperature in the prior art cannot control the pressure applied to the animal wound, and the scalding component is not sufficient to support wounds larger than 2 cm in diameter. Abroad, the research on burn modeling instruments is also in a similar situation. Researchers usually cannot ensure that model animals in different groups are in the same scald state, and lack systematic instrument-assisted modeling. The future development trend is bound to be towards a more mature and intelligent burn modeling instrument. Therefore, the research and development of a standardized device for animal burn modeling has economic and social benefits. Summary of the invention:

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art and design a standardized device for animal skin burn models with safe operation, good repeatability, and accurate control of burn area and depth, so as to ensure the accuracy of the burn model and the safety of experimenters and animals.

[0007] In order to achieve the above-mentioned purpose, the main structure of the standardized device for modeling animal skin burns involved in the present invention includes a main unit and a handle connected thereto, and a metal sheet connected to the handle through a metal rod, and the metal rod and the metal sheet are detachably connected; wherein the main unit has at least a temperature adjustment knob and a switch button as well as a temperature display screen and a pressure display screen; the handle has at least a pressure adjustment push rod, and at least a heating wire, a heat insulation layer, a pressure sensor and a spring are arranged inside.

[0008] The standardized device for modeling animal skin burns of the present invention can achieve consistency in modeling of the same batch, and meet the requirements of different burn modeling standards by accurately regulating temperature, pressure and time; the specific process of modeling is as follows:

[0009] First, the animals were anesthetized, disinfected, and shaved before surgery;

[0010] Then, a metal sheet of a set size and shape is mounted on the end of the metal rod;

[0011] Connect the power supply of the host, rotate the temperature knob to set the temperature, press the temperature knob to confirm the current temperature, and press the power button to turn on the machine;

[0012] When the temperature displayed on the temperature display reaches the set temperature, push the pressure adjustment push rod, and when the pressure displayed on the pressure display reaches the set value, stop pushing;

[0013] Finally, a metal sheet is placed on the set burn site to perform burn modeling;

[0014] During this process, the animal's reaction was closely observed to ensure safety. After the modeling was completed, the wound was treated (cold compress, cleaning and bandaging) to relieve pain and prevent infection.

[0015] The standardized device for animal skin burn modeling disclosed by the present invention is used for preparing a pig skin burn model at a temperature of 60-200°C, a pressure of 15-30N, and a scalding time of 30-60s;

[0016] The wound condition was observed on the third day after modeling, and photos were taken to analyze the burn extent. The burn tissues were taken for HE and Masson staining to verify the burn extent.

[0017] The wound is red, swollen, hot and painful, with clear wound edges and little exudate;

[0018] After HE staining, it can be seen that the morphology of the superficial dermis is abnormal, while the morphology of the deep dermis is normal, which can be judged as a deep second-degree burn.

[0019] Masson staining showed that the collagen fiber structure in the burn area was disordered, while the collagen morphology in the deep dermis was normal, which could be judged as a deep second-degree burn.

[0020] Compared with the prior art, the present invention controls the degree of burns by adjusting temperature, pressure, and shortening contact time to achieve first-degree or shallow second-degree burns. It can also adjust the shape and size of the metal sheet to perform burn modeling on animals such as mice or rats, especially to prepare a deep second-degree burn model. It can also ensure the safety of personnel, animals and equipment during the experiment through measures such as overheating protection and emergency shutdown. It can be widely used in multiple fields of basic medicine such as preclinical drug screening, treatment method evaluation, and analysis of burn pathological mechanisms to meet experimental needs, and is expected to become an important animal modeling instrument in basic burn research; it has a simple structure, and can control the degree of burns of experimental animals by setting parameters such as burn temperature, time and range to simulate burns of different degrees. It has strong operability and high repeatability, and the intra-batch and inter-batch differences in the prepared burn animal models are small, and it is an indispensable tool in burn modeling research. Description of the drawings:

[0021] Figure 1 It is a schematic diagram of the main structural principle of the present invention.

[0022] Figure 2 It is a comparative schematic diagram of the spring before and after expansion and contraction of the present invention.

[0023] Figure 3 The figure is a schematic diagram of the structural principle of the metal sheet involved in the present invention.

[0024] Figure 4 Schematic diagram of the wound of the burn model prepared in Examples 1-3 of the present invention.

[0025] Figure 5 Schematic diagram of HE staining results of the wound of the burn model prepared in Examples 1-3 of the present invention and normal skin.

[0026] Figure 6 Schematic diagram of Masson staining results of the wound of the burn model prepared in Examples 1-3 of the present invention and normal skin. Specific implementation method:

[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] Embodiment 1:

[0029] The standardized device for animal skin burn modeling involved in this embodiment can accurately burn large animals and prepare animal burn models with consistent burn degrees. The burn degrees can be verified by HE and Masson experiments.

[0030] Its main structure is as follows Figure 1 As shown, it includes a main unit 1, a handle 2, a metal rod 3, a metal sheet 4, a temperature adjustment knob 5, a switch button 6, a temperature display screen 7, a pressure display screen 8 and a pressure adjustment push rod 9;

[0031] The host 1 is connected to the handle 2, the handle 2 is connected to the metal rod 3, and the metal rod 3 is detachably connected to the metal sheet 4;

[0032] The host 1 has a temperature adjustment knob 5, a switch button 6, a temperature display screen 7 and a pressure display screen 8;

[0033] The handle 2 has a pressure regulating push rod 9, which has a heating wire, a heat insulation layer, a pressure sensor and a spring inside. The heating wire and the pressure sensor are electrically connected to the host 1 respectively, the pressure sensor is connected to the pressure regulating push rod 9, and the spring is mechanically connected to the pressure regulating push rod 9.

[0034] The host 1 involved in this embodiment adjusts the temperature by rotating the temperature adjustment knob 5, pressing the temperature adjustment knob 5 to confirm the set temperature, and turning it on and off by pressing the switch button 6. The current temperature is displayed on the temperature display screen 7, and the current pressure is displayed on the pressure display screen 8;

[0035] The handle 2 is made of wood. The heating wire heats the metal rod 3 and transfers the heat to the metal sheet 4, so that the metal rod 3 can burn the animal skin. The heat insulation layer can prevent the modeling personnel from being burned. The pressure sensor monitors the pressure of the pressure regulating push rod 9 and uploads it to the host 1 to display it through the pressure display screen 8. The expansion and contraction of the spring drives the metal rod 3 to expand and contract. Figure 2 As shown;

[0036] The size and shape of the metal sheet 4 vary, such as Figure 3 As shown, different wounds are prepared on the skin of different animals. For example, a circular metal sheet 4 with a diameter of 1 cm is used for mice, a metal sheet 4 with a diameter of 2 cm is used for rats or rabbits, and a metal sheet 4 with a diameter of 4 cm is used for large animals such as rhesus monkeys or pigs;

[0037] The pressure regulating push rod 9 adjusts the extension length (0-3 cm) of the metal rod 3 through the spring and applies a set pressure (0-30N).

[0038] When the standardized device for animal skin burn modeling involved in this embodiment is used, a metal sheet 4 with a diameter of 4 cm is installed on the end of the metal rod 3, the temperature is set to 95°C, and a scald is performed on a selected area of ​​the pig's back. Under a pressure of 15N, the metal sheet 4 is kept in contact with the pig's skin for 30 seconds to prepare a burn model.

[0039] Embodiment 2:

[0040] When the standardized device for animal skin burn modeling involved in this embodiment is used, a metal sheet 4 with a diameter of 4 cm is installed on the end of the metal rod 3, the temperature is set to 120°C, and a selected area on the pig's back is scalded. Under a pressure of 20N, the metal sheet 4 is kept in contact with the pig's skin for 30 seconds to prepare a burn model.

[0041] Embodiment 3:

[0042] When the standardized device for animal skin burn modeling involved in this embodiment is used, a metal sheet 4 with a diameter of 4 cm is installed on the end of the metal rod 3, the temperature is set to 150°C, and a scald is performed on a selected area of ​​the pig's back. Under a pressure of 30N, the metal sheet 4 is kept in contact with the pig's skin for 30 seconds to prepare a burn model.

[0043] Embodiment 4:

[0044] In this example, the burn models prepared in Examples 1-3 were verified for burn degree and it was found that all of them reached deep II degree burns; among them, the wound conditions on the day of modeling and on the 3rd day after modeling were as follows: Figure 4 The HE staining results are shown in Figure 5 The results of Masson staining are shown in Figure 6 shown.

Claims

1. A standardized device for animal skin burn modeling, characterized in that: The main structure comprises a main body and a handle connected thereto, and a metal sheet connected to the handle via a metal rod, wherein the metal rod and the metal sheet are detachably connected.

2. The standardized device for animal skin burn modeling according to claim 1, characterized in that: The host machine at least has a temperature adjustment knob and a switch button as well as a temperature display screen and a pressure display screen.

3. The standardized device for animal skin burn modeling according to claim 2, characterized in that: The handle at least has a pressure regulating push rod, and at least a heating wire, a heat insulating layer, a pressure sensor and a spring are arranged inside.

4. The standardized device for animal skin burn modeling according to claim 3, characterized in that: The specific process of modeling is as follows: First, the animals were subjected to preoperative procedures; Then, install the metal sheet on the end of the metal rod; Connect the host to the power supply, set the temperature, and turn on the machine; When the set temperature value is reached, push the pressure regulating push rod, and when the set pressure value is reached, stop pushing; Finally, burns were modeled on the animals using metal sheets.

5. The standardized device for animal skin burn modeling according to claim 3 or 4, characterized in that: When preparing the pig skin burn model, the temperature is 60-200°C, the pressure is 15-30N, and the scalding time is 30-60s.

Images