Whitening and acne-removing composition, preparation method and application thereof, whitening and acne-removing cosmetic and preparation method thereof

Abstract

The invention discloses a whitening and acne-removing composition, a preparation method and application thereof, a whitening and acne-removing cosmetic and a preparation method thereof. The whitening and acne-removing composition comprises a solvent and a whitening and acne-removing component dispersed in the solvent, the mass of the whitening and acne removing component accounts for 0.1-0.5% of the total mass of the whitening and acne removing composition, and the whitening and acne removing component comprises DNA sodium, mu-conopeptide and quaternary ammonium salt-73. According to the whitening and acne-removing composition as well as the preparation method and the application thereof, through scientific compounding of functional components, melanin formation can be inhibited through multi-target synergistic inhibition under the condition of low addition concentration, bacteria can be efficiently inhibited, and good whitening and acne-removing effects can be achieved; the composition is transparent clear liquid, has good thermal stability and solubility, is low in irritation and easy to formulate, can be used as a raw material to be added into cosmetic finished products, and is used for preparing cosmetics with whitening and acne removing effects.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and relates to a cosmetic component, in particular to a whitening and anti-acne composition and a preparation method and application thereof, a whitening and anti-acne cosmetic and a preparation method thereof. Background Art

[0002] From ancient times to now, fair and clean skin has always been the pursuit of Oriental women. Skin pigmentation caused by ultraviolet rays, inflammatory response and abnormal hormone levels is one of the main factors affecting skin color. Human skin color is mainly determined by melanin. Currently, whitening cosmetics on the market mainly reduce the formation of melanin by inhibiting melanin synthesis, reducing melanin, inhibiting melanosome transport, especially by inhibiting tyrosinase (TYR), the key rate-limiting enzyme in the melanin biosynthesis pathway, to achieve whitening effects.

[0003] In addition, acne caused by abnormal hormone levels, excessive secretion of sebaceous glands, proliferation of Propionibacterium acnes and Malassezia, abnormal keratinization of the hair follicle sebaceous gland ducts and inflammation are also important factors affecting the appearance of the skin. Acne is prone to pigmentation, which seriously affects the appearance of the skin. At present, acne-removing products mainly use salicylic acid to remove skin keratin, exposing anaerobic Propionibacterium acnes to the air, thereby inhibiting its activity. In addition, ethanol is used to remove excess oil from the skin, tighten pores, and increase the solubility of salicylic acid.

[0004] First, most of the cosmetics on the market currently have a single effect of whitening or acne removal; the technical solution explained in the Chinese invention patent CN202311158810.5 cannot take into account both whitening and acne removal effects; secondly, traditional whitening and acne removal ingredients such as phenethylresorcinol (SymWh ite377), salicylic acid and ethanol are highly irritating, and the composite plant extracts are complex in composition, which can easily cause skin irritation after use and are not suitable for long-term use; in addition, some whitening and acne removal compositions (such as the technical solution disclosed in the Chinese invention patent CN202211038113.1) have the disadvantages of strong volatility, which can easily lead to low bioavailability and unsatisfactory product effects when applied to cosmetics. Finally, traditional acne removal ingredients such as salicylic acid and composite plant extracts have poor solubility and thermal stability, and the plant extracts are dark in color, which will greatly increase the formulation restrictions and formulation difficulty when applied to the formulation.

[0005] In view of this, there is an urgent need to design a new cosmetic component with whitening and acne-removing effects so as to overcome at least part of the above-mentioned defects of existing cosmetic components. Summary of the invention

[0006] The present invention provides a whitening and anti-acne composition and a preparation method and application thereof, a whitening and anti-acne cosmetic and a preparation method thereof, which can synergistically inhibit melanin formation at multiple targets under low added concentration, effectively inhibit bacteria, and exert good whitening and anti-acne effects.

[0007] To solve the above technical problem, according to one aspect of the present invention, the following technical solution is adopted:

[0008] A whitening and anti-acne composition, comprising: a solvent and a whitening and anti-acne component dispersed in the solvent;

[0009] The weight of the whitening and anti-acne component accounts for 0.3-1.5% of the total weight of the whitening and anti-acne composition, and the whitening and anti-acne component includes sodium DNA, μ-conopeptide and quaternary ammonium salt-73.

[0010] As an embodiment of the present invention, the mass of the DNA sodium accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition, and is extracted from deep-sea salmon milt in an unpolluted area in the North Pacific Ocean;

[0011] The μ-conopeptide accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition and is a mini growth factor obtained by a polypeptide solid phase synthesis method; the molecular weight of the μ-conopeptide is 2000-2200Da;

[0012] The quaternary ammonium salt-73 accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition. The quaternary ammonium salt-73 has whitening effect and strong anti-acne effect.

[0013] As an embodiment of the present invention, the solvent is a 50% propylene glycol aqueous solution or a 50% butylene glycol aqueous solution.

[0014] According to another aspect of the present invention, the following technical scheme is adopted: a preparation method of a whitening and anti-acne composition, the preparation method comprising:

[0015] Step S1, weighing DNA sodium powder, mixing the DNA sodium powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a material-liquid mass ratio of 1: (9-199), heating, filtering, and cooling to obtain a DNA sodium extract, wherein the heating time is 1-2 hours;

[0016] Step S2, weighing quaternary ammonium salt-73 powder, mixing the quaternary ammonium salt-73 powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a feed-liquid mass ratio of 1: (9-199), heating, filtering, and cooling to obtain a quaternary ammonium salt-73 solution, wherein the heating time is 1-2 hours;

[0017] Step S3, weighing μ-conopeptide powder, mixing the μ-conopeptide powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a feed-liquid mass ratio of 1:(9-199), heating, filtering and cooling to obtain a μ-conopeptide solution, wherein the heating time is 1-2 hours;

[0018] Step S4, uniformly mix the extracts obtained in steps S1, S2, and S3 to obtain the whitening and anti-acne composition.

[0019] As an embodiment of the present invention, in steps S1, S2, and S3, the heating temperature is 60-80°C, and the cooling temperature is 30-40°C.

[0020] According to another aspect of the present invention, the following technical scheme is adopted: use of the above-mentioned whitening and anti-acne composition or the whitening and anti-acne composition prepared by the above-mentioned preparation method in the preparation of whitening and anti-acne cosmetics.

[0021] According to another aspect of the present invention, the following technical solution is adopted: a whitening and anti-acne cosmetic, wherein the whitening and anti-acne cosmetic comprises the above-mentioned whitening and anti-acne composition.

[0022] As an embodiment of the present invention, the whitening and anti-acne cosmetic is at least one of toner, lotion, essence, ointment, cream, facial mask, and freeze-dried powder;

[0023] The whitening and anti-acne cosmetics further comprise at least one of a moisturizer, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative;

[0024] The mass of the whitening and anti-acne composition accounts for 1-10% of the mass of the cosmetic.

[0025] As an embodiment of the present invention, the moisturizer comprises at least one of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, trehalose, betaine and panthenol; the skin conditioner comprises at least one of ceramide, allantoin, niacinamide and glucose; the emollient comprises at least one of squalene, beeswax, jojoba seed oil, castor oil, avocado oil and avocado butter; the emulsifier comprises at least one of cetearyl alcohol, stearic acid, cetearyl glucoside lanolin, lanolin, sorbitan stearate and sorbitan olivate; the thickener comprises at least one of xanthan gum, carbomer, cetyl alcohol, acrylates / C10-30 alkyl acrylate crosspolymer, sodium polyacrylate and cyclopentasiloxane; the preservative is phenoxyethanol.

[0026] According to another aspect of the present invention, the following technical scheme is adopted: a method for preparing the above-mentioned whitening and anti-acne cosmetics, the preparation method comprising the following steps:

[0027] (1) adding a moisturizer, a thickener and a skin conditioner into deionized water, heating and homogenizing, and stirring to obtain a mixed solution A;

[0028] (2) heating the emulsifier and the emollient, and after they are completely dissolved, adding them to the mixed solution A, and adding the preservative, homogenizing and stirring to obtain a mixed solution B;

[0029] (3) Cooling the mixed solution B, adding the whitening and anti-acne composition, homogenizing, stirring, and filtering.

[0030] The beneficial effects of the present invention are as follows: the whitening and anti-acne composition and its preparation method, application, whitening and anti-acne cosmetics and its preparation method proposed by the present invention can inhibit melanin formation at multiple targets in a low added concentration through scientific compounding of functional ingredients, and effectively inhibit bacteria, thereby exerting good whitening and anti-acne effects. The composition is a transparent and clear liquid, has good thermal stability and solubility, is low in irritation, is easy to formulate, can be added as a raw material to finished cosmetics, and is used to prepare cosmetics with whitening and anti-acne effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The present invention is a flowchart of a method for preparing a whitening and anti-acne composition according to an embodiment of the present invention.

[0032] Figure 2 The present invention is a flowchart of a method for preparing whitening and anti-acne cosmetics in one embodiment of the present invention. DETAILED DESCRIPTION

[0033] The preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0034] In order to further understand the present invention, preferred embodiments of the present invention are described below in conjunction with examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, rather than limiting the claims of the present invention.

[0035] The description in this section is only for several typical embodiments, and the present invention is not limited to the scope of the embodiments. The same or similar prior art means and some technical features in the embodiments are mutually replaced within the scope of the present invention.

[0036] Unless otherwise indicated, implied from the context, or customary in the prior art, all parts and percentages in this application are based on weight, and the test and characterization methods used are all current as of the filing date of this application. Where applicable, the contents of any patent, patent application or publication referred to in this application are fully incorporated herein by reference, and their equivalent patent families are also incorporated by reference, especially the definitions of synthesis techniques, product and processing designs, polymers, comonomers, initiators or catalysts disclosed in these documents in the art. If the definition of a specific term disclosed in the prior art is inconsistent with any definition provided in this application, the definition of the term provided in this application shall prevail.

[0037] Numerical ranges in this application are approximate values, so unless otherwise specified, they may include numerical values ​​outside the range. Numerical ranges include all numerical values ​​from the lower limit to the upper limit increased by 1 unit, provided that there is an interval of at least 2 units between any lower value and any higher value. For example, if the recorded component, physical or other properties (such as molecular weight, melt index, etc.) are 100 to 1000, it means that all single values ​​are clearly listed, such as 100, 101, 102, etc., and all sub-ranges, such as 100 to 166, 155 to 170, 198 to 200, etc. For a range containing a numerical value less than 1 or containing a fraction greater than 1 (such as 1.1, 1.5, etc.), 1 unit is appropriately regarded as 0.0001, 0.001, 0.01 or 0.1. For a range containing a single digit less than 10 (such as 1 to 5), 1 unit is usually regarded as 0.1. These are only specific examples of what is intended to be expressed, and all possible combinations of values ​​between the lowest and highest values ​​listed are considered to be clearly recorded in this application. It should also be noted that the terms "first", "second", etc. in this article do not limit the order of precedence, but are only used to distinguish substances with different structures.

[0038] When used with respect to chemical compounds, unless expressly specified otherwise, the singular includes all isomeric forms and vice versa (e.g., "hexane" includes all isomers of hexane, individually or collectively). In addition, nouns using "a," "an," or "the" also include their plural forms unless expressly specified otherwise.

[0039] The terms "comprising", "including", "having" and their derivatives do not exclude the presence of any other components, steps or processes, and are irrelevant to whether these other components, steps or processes are disclosed in this application. To eliminate any doubt, unless explicitly stated, all compositions using the terms "comprising", "including", or "having" in this application may include any additional additives, adjuvants or compounds. On the contrary, except for those necessary for operating performance, the term "essentially consisting of..." excludes any other components, steps or processes from the scope of any description of the term below. The term "consisting of..." does not include any components, steps or processes that are not specifically described or listed. Unless explicitly stated, the term "or" refers to the listed individual members or any combination thereof.

[0040] The description of the steps in each embodiment in the specification is only for the convenience of explanation, and the implementation method of the present application is not limited by the order of implementation of the steps.

[0041] The invention discloses a whitening and anti-acne composition, which comprises: a solvent and a whitening and anti-acne component dispersed in the solvent; the mass of the whitening and anti-acne component accounts for 0.3-1.5% of the total mass of the whitening and anti-acne composition, and the whitening and anti-acne component comprises sodium DNA, μ-conopeptide and quaternary ammonium salt-73.

[0042] In one embodiment of the present invention, the mass of the DNA sodium accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition, and can be extracted from deep-sea salmon milt in the unpolluted area of ​​the North Pacific Ocean. The μ-conopeptide accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition, and the μ-conopeptide is a mini growth factor obtained by a polypeptide solid phase synthesis method. The quaternary ammonium salt-73 accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition, and the quaternary ammonium salt-73 is a multifunctional ingredient with a structure similar to cyanine dyes, which has a whitening effect similar to kojic acid and a strong acne removal effect. The solvent can be a 50% propylene glycol aqueous solution or a 50% butylene glycol aqueous solution. In one embodiment, the solvent can be a 1,2-propylene glycol solution.

[0043] Through the above technical scheme, DNA sodium is extracted from deep-sea salmon milt in the unpolluted area of ​​the North Pacific Ocean. The fish sperm is highly mature and rich in DNA nucleic acid. Using it as a cosmetic raw material can inhibit the activity of tyrosinase by chelating copper ions to prevent the substrate from binding to tyrosinase, thereby achieving a whitening effect. In addition, it has the effect of absorbing UVB and preventing ultraviolet damage. Due to its large molecular weight, it will not penetrate into the skin, so it has good safety and stability. In addition, as a nutrient, DNA sodium has the effect of repairing scar pigmentation, which helps to whiten and repair the skin after acne removal. μ-conopeptide is a mini growth factor obtained by peptide solid phase synthesis. It can induce fibroblast differentiation and regeneration through the TGF-β pathway, increase dermal density and thickness, reduce wrinkles, and improve skin color and uniformity, whitening and enhancing synergy. At the same time, it can promote skin tolerance and reduce the irritation of other ingredients to the skin. Quaternary ammonium salt-73 is a multifunctional ingredient with a structure similar to cyanine dyes. It has a whitening effect similar to kojic acid, can inhibit melanin production, and play a synergistic whitening effect. In addition, it can achieve a broad-spectrum antibacterial effect by destroying bacterial cell membranes and promoting bacterial intercellular degradation. It can effectively inhibit Propionibacterium acnes at low dosages and has a powerful acne-removing effect. Sodium DNA, μ-conopeptide and quaternary ammonium salt-73 can synergistically inhibit melanin formation at low added concentrations, effectively inhibit bacteria, and exert a powerful whitening and acne-removing effect.

[0044] The present invention also discloses a method for preparing a whitening and anti-acne composition. Figure 1 is a flow chart of a method for preparing a whitening and anti-acne composition according to an embodiment of the present invention; please refer to Figure 1 , the preparation method comprises:

[0045] [Step S1] Weighing sodium DNA powder, mixing the sodium DNA powder with a 50% propylene glycol aqueous solution or a 50% butylene glycol aqueous solution at a material-liquid mass ratio of 1:(9-199), heating, filtering, and cooling to obtain a sodium DNA extract, wherein the heating time is 1-2 hours;

[0046] [Step S2] Weigh quaternary ammonium salt-73 powder, mix the quaternary ammonium salt-73 powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a feed-liquid mass ratio of 1:(9-199), heat, filter, and cool to obtain a quaternary ammonium salt-73 solution, wherein the heating time is 1-2 hours;

[0047] [Step S3] Weighing μ-conopeptide powder, mixing the μ-conopeptide powder with a 50% propylene glycol aqueous solution or a 50% butylene glycol aqueous solution at a material-liquid mass ratio of 1:(9-199), heating, filtering, and cooling to obtain a μ-conopeptide solution, wherein the heating time is 1-2 hours;

[0048] [Step S4] The extracts prepared in steps S1, S2, and S3 are mixed evenly to obtain the whitening and anti-acne composition.

[0049] In one embodiment of the present invention, in steps S1, S2, and S3, the heating temperature is 60-80° C., and the cooling temperature is 30-40° C. The present invention also discloses a use of the above-mentioned whitening and anti-acne composition or the whitening and anti-acne composition prepared by the above-mentioned preparation method in the preparation of whitening and anti-acne cosmetics.

[0050] The present invention further discloses a whitening and anti-acne cosmetic, the cosmetic comprising the above-mentioned whitening and anti-acne composition. In one embodiment of the present invention, the mass of the whitening and anti-acne composition accounts for 1-10% of the mass of the cosmetic. The whitening and anti-acne cosmetic is at least one of toner, lotion, essence, ointment, cream, facial mask, and freeze-dried powder. The mass of the whitening and anti-acne composition accounts for 1-10% of the mass of the cosmetic.

[0051] The whitening and anti-acne cosmetics may also include at least one of a moisturizer, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative. The moisturizer includes at least one of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, trehalose, betaine, and panthenol; the skin conditioner includes at least one of ceramide, allantoin, niacinamide, and glucose; the emollient includes at least one of squalene, beeswax, jojoba seed oil, castor oil, avocado oil, and avocado butter; the emulsifier includes at least one of cetearyl alcohol, stearic acid, cetearyl glucoside lanolin, lanolin, sorbitan stearate, and sorbitan olivate; the thickener includes at least one of xanthan gum, carbomer, cetyl alcohol, acrylates / C10-30 alkyl acrylate crosspolymer, sodium polyacrylate, and cyclopentasiloxane; the preservative is phenoxyethanol.

[0052] The present invention below sets various groups of embodiments and comparative examples using a job interview as an example, but is not limited to the use of facial creams. The composition can also be applied to various skin care products such as toners, emulsions, essences, creams, facial masks, freeze-dried powders, etc.

[0053] Table 1 Components and dosage of Examples 1-5

[0054]

[0055]

[0056] The preparation method of the whitening and anti-acne composition is as follows:

[0057] Step S1, weighing 1 part (by mass, the same below) of sodium DNA powder, adding 99 parts of 50% propylene glycol aqueous solution, heating, filtering, cooling, and preparing a sodium DNA extract, wherein the heating time is 1 to 2 hours;

[0058] Step S2, weighing 1 part of quaternary ammonium salt-73 powder, adding 99 parts of 50% propylene glycol aqueous solution, heating, filtering, cooling, and preparing quaternary ammonium salt-73 solution, wherein the heating time is 1 to 2 hours;

[0059] Step S3, weighing 1 part of the μ-conopeptide solution, adding 99 parts of a 50% propylene glycol aqueous solution, heating, filtering, and cooling to obtain a μ-conopeptide solution, wherein the heating time is 1 to 2 hours;

[0060] Step S4, uniformly mixing the extracts obtained in step S1, step S2 and step S3 to obtain the whitening and anti-acne composition.

[0061] In one embodiment of the present invention, the heating temperature in steps S1 to S3 is 60-80°C; the cooling temperature in steps S1 to S3 is 30-40°C.

[0062] The present invention also discloses a method for preparing a whitening and anti-acne cosmetic. Figure 2 is a flow chart of a method for preparing a whitening and anti-acne cosmetic according to an embodiment of the present invention; Figure 2 , the preparation method comprises the following steps:

[0063] Step 1, adding a moisturizer, a thickener and a skin conditioner into deionized water, heating, homogenizing and stirring to obtain a mixed solution A;

[0064] Step 2, heating the emulsifier and the emollient, and after they are completely dissolved, adding them to the mixed solution A, and adding the preservative, homogenizing and stirring to obtain a mixed solution B;

[0065] Step 3: Cool the mixed solution B, add the whitening and anti-acne composition, homogenize, stir, and filter.

[0066] In one embodiment of the present invention, the desired whitening and anti-acne cream is obtained by filtering and sterilizing before filling, detecting the bacterial content, and performing quality control on toxins, immunogenicity, etc.

[0067] The heating temperature in step 1 and step 2 can be 75-80°C; the temperature is lowered to 35-38°C in step 3; the stirring speed in steps 1 to 3 is 200-400 rpm; the homogenization speed in steps 1 to 3 is 2000-4000 rpm.

[0068] Example 1

[0069] An embodiment of the whitening and anti-acne composition and the whitening and anti-acne cream of the present invention comprises 0.3% of whitening and anti-acne effective ingredients by total weight of the composition, wherein the content of sodium DNA is 0.1%, the content of μ-conopeptide is 0.1%, and the content of quaternary ammonium salt-73 is 0.1%.

[0070] Example 2

[0071] An embodiment of the whitening and anti-acne composition and the whitening and anti-acne cream of the present invention comprises 0.6% of whitening and anti-acne effective ingredients by total weight of the composition, wherein the content of sodium DNA is 0.2%, the content of μ-conopeptide is 0.2%, and the content of quaternary ammonium salt-73 is 0.2%.

[0072] Example 3

[0073] An embodiment of the whitening and anti-acne composition and the whitening and anti-acne cream of the present invention comprises 0.9% of whitening and anti-acne effective ingredients by total weight of the composition, wherein the content of sodium DNA is 0.3%, the content of μ-conopeptide is 0.3%, and the content of quaternary ammonium salt-73 is 0.3%.

[0074] Example 4

[0075] An embodiment of the whitening and anti-acne composition and the whitening and anti-acne cream of the present invention comprises 1.2% of whitening and anti-acne effective ingredients by total weight of the composition, wherein the content of sodium DNA is 0.4%, the content of μ-conopeptide is 0.4%, and the content of quaternary ammonium salt-73 is 0.4%.

[0076] Example 5

[0077] An embodiment of the whitening and anti-acne composition and the whitening and anti-acne cream of the present invention comprises 1.5% of whitening and anti-acne effective ingredients by total weight of the composition, wherein the content of sodium DNA is 0.5%, the content of μ-conopeptide is 0.5%, and the content of quaternary ammonium salt-73 is 0.5%.

[0078] Comparative Example 1

[0079] Control Example 1 provides a preparation method of a whitening and anti-acne composition and a whitening and anti-acne cream, which is different from Example 1 in that the proportions of the effective ingredients of the whitening and anti-acne composition are different during the preparation process, wherein the content of sodium DNA is 0.001%, the content of μ-conopeptide is 0.0005%, and the content of quaternary ammonium salt-73 is 0.0015%.

[0080] Comparative Example 2

[0081] Control Example 2 provides a method for preparing a whitening and anti-acne composition and a whitening and anti-acne cream, which is different from Example 1 in that the proportions of the effective ingredients of the whitening and anti-acne composition are different during the preparation process, wherein the content of sodium DNA is 0.001%, the content of μ-conopeptide is 0.0015%, and the content of quaternary ammonium salt-73 is 0.0005%.

[0082] Comparative Example 3

[0083] Control Example 3 provides a preparation method of a whitening and anti-acne composition and a whitening and anti-acne cream, which is different from Example 1 in that the proportions of the effective ingredients of the whitening and anti-acne composition are different during the preparation process, wherein the content of sodium DNA is 0.0005%, the content of μ-conopeptide is 0.001%, and the content of quaternary ammonium salt-73 is 0.0015%.

[0084] Comparative Example 4

[0085] Control Example 4 is a commercially available cream that claims to have acne-removing and whitening effects and is an alternative to the whitening and acne-removing cream. The product claims that the effective ingredients are niacinamide, salicylic acid, and glycolic acid. After testing, the product contains 0.5%, 0.2%, and 0.001% respectively.

[0086] Characterization data and effect data of the products of the embodiments and comparative examples

[0087] 1. Determination of tyrosinase activity in human skin melanocytes

[0088] Test product: The whitening and anti-acne creams obtained in Examples 1 to 5 and Control Examples 1 to 4 were diluted to 100 mg / mL with ethanol solution.

[0089] Materials: Human skin melanocytes.

[0090] 2. Malassezia inhibition test

[0091] Test product: the whitening and anti-acne facial cream obtained in Examples 1 to 5 and Control Examples 1 to 4.

[0092] Species: Malassezia furfur.

[0093] 3. Propionibacterium inhibition test

[0094] Test product: the whitening and anti-acne facial cream obtained in Examples 1 to 5 and Control Examples 1 to 4.

[0095] Bacteria species: Propionibacterium acnes.

[0096] 4. Free radical (DPPH) scavenging test

[0097] Test product: the whitening and anti-acne facial cream obtained in Examples 1 to 5 and Control Examples 1 to 4.

[0098] Table 1 Comparison of the performance of the whitening and anti-acne creams obtained in Examples 1 to 5 and Comparative Examples 1 to 4

[0099]

[0100] As shown in Table 1, no Malassezia and Propionibacterium were detected in Examples 1-5, indicating that the whitening and anti-acne cream obtained by the present invention has a significant inhibitory effect on Malassezia and Propionibacterium, and also has a significant inhibitory effect on tyrosinase, which can reduce the production of melanin, indicating that the whitening and anti-acne cream has a good whitening effect.

[0101] 5. Human trials

[0102] Subjects: About 30 subjects aged 18 to 60 years old were selected for each group of examples, and skin lesion counts and ISGA scores were performed before and after using the whitening and anti-acne cream.

[0103] Test method: Based on "Q / CX001-2022 Test Method for Anti-Acne Effect of Cosmetics".

[0104] Test product: the whitening and anti-acne facial cream obtained in Examples 1 to 5 and Control Examples 1 to 4.

[0105] Statistical methods:

[0106] Skin lesion reduction percentage (%) = (ISGA score before use - ISGA score after use) / ISGA score before use * 100%.

[0107] Skin lesion regression rate (%) = (number of skin lesions before use - number of skin lesions after use) / number of skin lesions before use * 100%.

[0108] Table 2 ISGA evaluation criteria

[0109]

[0110] Table 3 Acne removal data results of whitening and anti-acne creams obtained in Examples 1 to 5 and Comparative Examples 1 to 4

[0111] Test Project Skin lesion reduction percentage (%) Skin lesion regression rate (%) Example 1 50.32 63.45 Example 2 53.22 67.24 Example 3 56.19 70.19 Example 4 60.74 75.01 Example 5 63.09 79.82 Comparative Example 1 51.74 62,75 Comparative Example 2 50.88 59.63 Comparative Example 3 49.31 64.08 Comparative Example 4 55.72 68.71

[0112] The results of human trials show that:

[0113] The skin lesion counts of the subjects in Example 1 before and after using the whitening and anti-acne cream were statistically improved, with a skin lesion reduction rate of 50.23% and a skin lesion regression rate of 63.45%. The whitening and anti-acne cream has an acne-removing effect.

[0114] The skin lesion counts of the subjects in Example 2 before and after using the whitening and anti-acne cream were statistically improved, with a skin lesion reduction rate of 53.22% and a skin lesion regression rate of 67.24%. The whitening and anti-acne cream has an acne-removing effect.

[0115] The skin lesion counts of the subjects in Example 3 before and after using the whitening and anti-acne cream were statistically improved, with a skin lesion reduction rate of 56.19% and a skin lesion regression rate of 70.19%. The whitening and anti-acne cream has an acne-removing effect.

[0116] The skin lesion counts of the subjects in Example 4 before and after using the whitening and anti-acne cream were statistically improved, with a skin lesion reduction rate of 60.74% and a skin lesion regression rate of 75.01%. The whitening and anti-acne cream has an acne-removing effect.

[0117] The skin lesion counts of the subjects in Example 5 before and after using the whitening and anti-acne cream were statistically improved, with a skin lesion reduction rate of 63.09% and a skin lesion regression rate of 79.82%. The whitening and anti-acne cream has an acne-removing effect.

[0118] In summary, the whitening and anti-acne composition and its preparation method, application, whitening and anti-acne cosmetics and their preparation method proposed in the present invention can inhibit melanin formation at multiple targets in a low added concentration through scientific compounding of functional ingredients, and effectively inhibit bacteria, thereby exerting good whitening and anti-acne effects. The composition is a transparent and clear liquid, has good thermal stability and solubility, is low in irritation, is easy to formulate, and can be added as a raw material to finished cosmetics for the preparation of cosmetics with whitening and anti-acne effects.

[0119] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0120] The description and application of the present invention here are illustrative, and it is not intended to limit the scope of the present invention to the above-mentioned embodiments. The effects or advantages involved in the embodiments may not be embodied in the embodiments due to interference from various factors, and the description of the effects or advantages is not used to limit the embodiments. The deformation and change of the embodiments disclosed here are possible, and the replacement of the embodiments and the various equivalent parts are well known to those of ordinary skill in the art. It should be clear to those skilled in the art that the present invention can be implemented in other forms, structures, arrangements, proportions, and with other components, materials and parts without departing from the spirit or essential features of the present invention. Other deformations and changes can be made to the embodiments disclosed here without departing from the scope and spirit of the present invention.

Claims

1. A whitening and anti-acne composition, characterized in that: The whitening and anti-acne composition comprises: a solvent and a whitening and anti-acne component dispersed in the solvent; The weight of the whitening and anti-acne component accounts for 0.3-1.5% of the total weight of the whitening and anti-acne composition, and the whitening and anti-acne component includes sodium DNA, μ-conopeptide and quaternary ammonium salt-73.

2. The whitening and anti-acne composition according to claim 1, characterized in that: The DNA sodium accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition, and is extracted from deep-sea salmon milt in an unpolluted area in the North Pacific Ocean; The μ-conopeptide accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition and is a mini growth factor obtained by a polypeptide solid phase synthesis method; the molecular weight of the μ-conopeptide is 2000-2200Da; The quaternary ammonium salt-73 accounts for 0.1-0.5% of the total mass of the whitening and anti-acne composition. The quaternary ammonium salt-73 has whitening effect and strong anti-acne effect.

3. The whitening and anti-acne composition according to claim 1, characterized in that: The solvent is a 50% propylene glycol aqueous solution or a 50% butylene glycol aqueous solution.

4. A method for preparing a whitening and anti-acne composition, characterized in that: The preparation method comprises: Step S1, weighing DNA sodium powder, mixing the DNA sodium powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a material-liquid mass ratio of 1: (9-199), heating, filtering, and cooling to obtain a DNA sodium extract, wherein the heating time is 1-2 hours; Step S2, weighing quaternary ammonium salt-73 powder, mixing the quaternary ammonium salt-73 powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a feed-liquid mass ratio of 1: (9-199), heating, filtering, and cooling to obtain a quaternary ammonium salt-73 solution, wherein the heating time is 1-2 hours; Step S3, weighing μ-conopeptide powder, mixing the μ-conopeptide powder with 50% propylene glycol aqueous solution or 50% butylene glycol aqueous solution at a feed-liquid mass ratio of 1:(9-199), heating, filtering and cooling to obtain a μ-conopeptide solution, wherein the heating time is 1-2 hours; Step S4, uniformly mix the extracts obtained in steps S1, S2, and S3 to obtain the whitening and anti-acne composition.

5. The preparation method according to claim 4, characterized in that: In steps S1, S2 and S3, the heating temperature is 60-80°C and the cooling temperature is 30-40°C.

6. Use of the whitening and anti-acne composition according to any one of claims 1 to 3 or the whitening and anti-acne composition prepared by the preparation method according to claim 4 or 5 in the preparation of whitening and anti-acne cosmetics.

7. A whitening and anti-acne cosmetic, characterized in that: The whitening and anti-acne cosmetic comprises the whitening and anti-acne composition according to any one of claims 1 to 3.

8. The whitening and anti-acne cosmetic according to claim 7, characterized in that: The whitening and anti-acne cosmetic is at least one of toner, lotion, essence, ointment, cream, facial mask and freeze-dried powder; The whitening and anti-acne cosmetics further comprise at least one of a moisturizer, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative; The mass of the whitening and anti-acne composition accounts for 1-10% of the mass of the cosmetic.

9. The whitening and anti-acne cosmetic according to claim 8, characterized in that: The moisturizer comprises at least one of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, trehalose, betaine and panthenol; the skin conditioner comprises at least one of ceramide, allantoin, niacinamide and glucose; the emollient comprises at least one of squalene, beeswax, jojoba seed oil, castor oil, avocado oil and avocado butter; the emulsifier comprises at least one of cetearyl alcohol, stearic acid, cetearyl glucoside lanolin, lanolin, sorbitan stearate and sorbitan olivate; the thickener comprises at least one of xanthan gum, carbomer, cetyl alcohol, acrylates / C10-30 alkyl acrylate crosspolymer, sodium polyacrylate and cyclopentasiloxane; the preservative is phenoxyethanol.

10. A method for preparing the whitening and anti-acne cosmetic according to any one of claims 7 to 9, characterized in that: The preparation method comprises the following steps: Step 1, adding a moisturizer, a thickener and a skin conditioner into deionized water, heating, homogenizing and stirring to obtain a mixed solution A; Step 2, heating the emulsifier and the emollient, and after they are completely dissolved, adding them to the mixed solution A, and adding the preservative, homogenizing and stirring to obtain a mixed solution B; Step 3: Cool the mixed solution B, add the whitening and anti-acne composition, homogenize, stir, and filter.

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