Preservation equipment and preservation treatment method for exosomes

Abstract

The invention provides preservation equipment and a preservation treatment method for exosomes, and relates to the technical field of storage device.The preservation equipment comprises a main body, a supporting part used for positioning a test tube is installed in the main body, and a positioning mechanism used for assisting in clamping the test tube is installed in the supporting part; the positioning mechanism comprises an auxiliary supporting rod for positioning a test tube and a limiting supporting rod for limiting, and one end of the auxiliary supporting rod is rotationally connected with a roller; the supporting part comprises a positioning sleeve, a connecting plate and a supporting base, and the other end of the auxiliary supporting rod is rotationally connected to the connecting plate. By arranging the positioning mechanism and arranging the pneumatic telescopic rod in the positioning mechanism, after the test tube containing the exosome is effectively positioned, by extending the pneumatic telescopic rod, on one hand, the clamping force on the test tube can be further increased, and on the other hand, the deformation space of the spring can be well compressed; and the test tube positioning effect can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of storage devices, and in particular to a storage device and a storage processing method for exosomes. Background Art

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins, specifically disc-shaped vesicles with a diameter of 40-100nm. In 1983, exosomes were first discovered in sheep reticulocytes. A variety of cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of lysosomal particles in cells, and are released into the extracellular matrix after the fusion of the outer membrane of the multivesicular body with the cell membrane. After the exosomes were first discovered, they were considered to be a way for cells to excrete waste. Now, with a large number of studies on their biological sources, their material composition and transport, the conduction of intercellular signals, and their distribution in body fluids, it is found that exosomes have a variety of functions. The function of exosomes depends on the cell type from which they come. They can participate in the body's immune response, antigen presentation, cell migration, cell differentiation, tumor invasion and other aspects.

[0003] Exosomes are usually stored in special storage devices to ensure the activity of the exosomes. When the exosomes are needed, they can be directly taken out from the storage device. Since the exosomes need to be stored in a low-temperature environment, in order to ensure that the storage environment of other exosomes in the storage device is not destroyed, the existing exosome storage device is provided with a special loading and unloading structure, which can only take out one test tube containing exosomes at a time, which can well ensure that the storage environment of other exosomes will not be affected. However, there will be new problems, that is, the efficiency of loading and unloading exosomes is too low. At the same time, when positioning and clamping the test tube containing exosomes, most of them will use the elastic force generated by the deformation of the spring to position the test tube containing exosomes. Although the positioning effect can be achieved to a certain extent, after the positioning is completed, the spring still has a deformation space for movement. When encountering severe vibration, it is easy to cause the original positioning effect to fail, and then cause the test tube storing the exosomes to be damaged. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides a preservation device and a preservation processing method for exosomes, which solves the problem that in order to ensure that the storage environment of other exosomes in the storage device is not destroyed, the existing exosome storage device is provided with a special taking and placing structure, which can only take out one test tube containing exosomes at a time, and can well ensure that the storage environment of other exosomes will not be affected. However, this will also lead to new problems, that is, the efficiency of taking and placing exosomes is too low. At the same time, when positioning and clamping the test tube containing exosomes, most of the time, the elastic force generated by the deformation of the spring is used to position the test tube containing exosomes. Although the positioning effect can be achieved to a certain extent, after the positioning is completed, the spring still has a deformation space for movement. When encountering severe vibration, it is easy to cause the original positioning effect to fail, and then cause the test tube storing the exosomes to be damaged.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a preservation device and a preservation processing method for exosomes, comprising a main body, a support part for positioning a test tube is installed inside the main body, a driving mechanism for driving the support part to move is installed inside the main body, a positioning mechanism for assisting in clamping the test tube is installed inside the support part, and a refrigeration chamber is fixedly connected to the main body; the positioning mechanism comprises an auxiliary support rod for positioning the test tube and a limiting support rod for limiting, one end of the auxiliary support rod is rotatably connected to a roller; the support part comprises a positioning sleeve, a connecting plate and a supporting base, and the other end of the auxiliary support rod is rotatably connected to the connecting plate.

[0008] Furthermore, the positioning mechanism also includes a guide rail, a moving block, a spring, a support plate, a pneumatic telescopic rod and a pulley, the roller is symmetrically connected to the moving block, the moving block is fixedly connected to the top of the spring, the support plate is fixedly connected to the bottom of the spring, and the pulley is slidably connected to the inside of the guide rail.

[0009] Furthermore, one end of the limiting support rod is rotatably connected to the auxiliary support rod, the other end of the limiting support rod is rotatably connected to the moving block, the pneumatic telescopic rod is fixedly connected to the connecting plate, and the support plate is fixedly connected to the telescopic end of the pneumatic telescopic rod.

[0010] Furthermore, the positioning sleeve is fixedly connected to the top of the connecting plate, the supporting base is fixedly connected to the bottom of the connecting plate, and the connecting plates are distributed on the supporting base in a circular array.

[0011] Furthermore, the driving mechanism includes a motor, an electromagnetic clutch, a driving gear, a driven gear, a screw and a nut. The motor is fixedly connected to the inner bottom of the main body, the electromagnetic clutch is fixedly connected to the output end of the motor, and the end of the electromagnetic clutch away from the motor is fixedly connected to a rotating shaft, and the top of the rotating shaft is rotatably connected to the bottom of the refrigeration chamber.

[0012] Furthermore, the driven gear is slidably connected to the screw rod, the driving gear is fixedly connected to the rotating shaft, the driving gear is meshed with the driven gear, the nut is threadedly connected to the screw rod, and the nut is connected to the support portion.

[0013] Furthermore, a switching mechanism is provided on the screw rod, and the switching mechanism includes a slide groove and an electric push rod, the electric push rod is fixedly connected to the surface of the screw rod, the slide groove is opened on the surface of the screw rod, the telescopic end of the electric push rod is fixedly connected to the bottom of the driven gear, and a limit block is fixedly connected to the driven gear, and the limit block cooperates with the slide groove.

[0014] Furthermore, a slide rail is fixedly connected to the inner surface of the main body, a slider is fixedly connected to the support portion, the slider is slidably connected to the inside of the slide rail, and the slider cooperates with the slide rail.

[0015] A method for preserving exosomes, comprising the following steps:

[0016] Step 1: Put the refrigerated iron block into the refrigeration chamber 3 and pre-cool the interior of the main body 1 in advance;

[0017] Step 2: Use the driving mechanism to extend the support part 2 to the outside of the main body 1, then place the test tube containing exosomes on the support part 2, and then the support part 2 descends to the inside of the main body 1;

[0018] Step 3: When it is necessary to take out the test tube containing the designated exosomes, the corresponding driven gear 11 is controlled to mesh with the driving gear 6 , thereby controlling the support portion 2 to extend out of the main body 1 .

[0019] Furthermore, in the step 2, a protective agent needs to be added to the test tube containing the exosomes. During the process of adding the protective agent, the protective agent is added by inserting a syringe into the rubber sealing plug that seals the test tube.

[0020] (III) Beneficial effects

[0021] The present invention provides a storage device and storage method for exosomes, which have the following beneficial effects:

[0022] 1. In this scheme, by setting a positioning mechanism and providing a pneumatic telescopic rod in the positioning mechanism, after the test tube containing exosomes is effectively positioned, the pneumatic telescopic rod can be extended to further increase the clamping force on the test tube on the one hand, and on the other hand, the deformation space of the spring can be well compressed, which is beneficial to improve the positioning effect of the test tube.

[0023] 2. In this scheme, by installing a roller on the auxiliary support rod, the friction generated when taking and placing the test tube can be effectively reduced, which is conducive to improving the convenience of taking and placing the test tube. In addition, the side surface of the roller is set to a concave arc shape, which can improve the stability of positioning the test tube containing exosomes.

[0024] 3. In this scheme, by setting a switching mechanism on the driving mechanism, the number of driven gears meshing with the driving gear can be adjusted, so that it is possible to effectively take and place only one test tube at a time, or to take and place multiple test tubes at a time, which is beneficial to improving the efficiency and flexibility of taking and placing the test tubes containing exosomes.

[0025] 4. In this scheme, by using a syringe to add a protective agent to the test tube containing exosomes after sealing, the contact between the exosomes in the test tube and the outside air can be effectively avoided, which is conducive to reducing the risk of cross infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A three-dimensional diagram of the external structure of a storage device and storage method for exosomes proposed by the present invention;

[0027] Figure 2 This is a schematic diagram of the internal structure of a storage device and storage processing method for exosomes proposed in the present invention;

[0028] Figure 3 This is a schematic diagram of the support structure of a storage device and storage method for exosomes proposed in the present invention;

[0029] Figure 4 This is a schematic diagram of the positioning mechanism structure of a storage device and storage processing method for exosomes proposed by the present invention;

[0030] Figure 5 The present invention proposes Figure 2 A magnified view of middle;

[0031] Figure 6 The present invention proposes Figure 4 Enlarged view of B.

[0032] Among them, 1. main body; 2. support part; 3. refrigeration chamber; 4. motor; 5. electromagnetic clutch; 6. driving gear; 7. screw rod; 8. slide rail; 9. positioning plate; 10. nut; 11. driven gear; 12. electric push rod; 13. rotating shaft; 14. slide groove;

[0033] 201, positioning sleeve; 202, connecting plate; 203, supporting base; 204, auxiliary support rod; 205, roller; 206, limiting support rod; 207, guide rail; 208, moving block; 209, spring; 2010, supporting plate; 2011, pneumatic telescopic rod; 2012, pulley. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] like Figure 1-6 As shown, an embodiment of the present invention provides a preservation device and a preservation processing method for exosomes, including a main body 1, a support part 2 for positioning a test tube is installed inside the main body 1, a driving mechanism for driving the support part 2 to move is installed inside the main body 1, a positioning mechanism for auxiliary clamping of the test tube is installed inside the support part 2, and a refrigeration chamber 3 is fixedly connected to the main body 1; the positioning mechanism includes an auxiliary support rod 204 for positioning the test tube and a limiting support rod 206 for limiting, and one end of the auxiliary support rod 204 is rotatably connected to a roller 205; the support part 2 includes a positioning sleeve 201, a connecting plate 202 and a supporting base 203, the other end of the auxiliary support rod 204 is rotatably connected to the connecting plate 202, the number of the connecting plates 202 is four, and the four connecting plates 202 are distributed in a circular array according to the center of the supporting base 203;

[0036] When the test tube containing exosomes is placed in the support part 2, the bottom of the test tube will squeeze the roller 205 installed on the end of the auxiliary support rod 204, and push the auxiliary support rod 204 installed on the connecting plate 202 to rotate downward, and at the same time push the limiting support rod 206 installed on the auxiliary support rod 204 to move downward, so that on the one hand, the stability of the movement of the auxiliary support rod 204 can be improved, and on the other hand, a supporting force can be provided for the auxiliary support rod 204, thereby providing a clamping force for positioning the test tube containing exosomes. The material of the roller 205 is made of rubber material, and the side surface of the roller 205 is set to an inwardly concave arc shape, which can improve the stability of positioning the test tube containing exosomes;

[0037] When the limiting support rod 206 moves downward, it will drive the moving block 208 installed at the end of the limiting support rod 206 to move along the guide rail 207 installed on the connecting plate 202. The symmetrical installation of the guide rail 207 can effectively ensure the stability of the movement and prevent the moving block 208 from falling off the guide rail 207. The moving block 208 is symmetrically connected to the pulley 2012 for rotation. The pulley 2012 cooperates with the guide rail 207. A triangular structure is formed between the limiting support rod 206, the auxiliary support rod 204 and the connecting plate 202, which can effectively improve the stability of the support for the auxiliary support rod 204.

[0038] As the moving block 208 moves downward, it squeezes the spring 209 fixed at the bottom of the moving block 208. The elastic force generated by squeezing the spring 209 provides a pressure source for clamping the test tube containing the exosomes. As the spring 209 is compressed, the moving block 208 slides on the guide rod, and the compressed spring 209 can be guided at the same time.

[0039] Then, the telescopic end of the pneumatic telescopic rod 2011 is extended to drive the support plate 2010 installed at the bottom of the spring 209 to move upward, so as to further compress the spring 209, thereby increasing the elastic force generated by the spring 209, further increasing the pressure on the test tube containing the exosomes, and thus improving the stability of the positioning of the test tube containing the exosomes. The support plate 2010 is symmetrically mounted with guide rods, the top of which passes through the moving block 208, the moving block 208 is slidably connected to the guide rod, and the spring 209 is sleeved on the outside of the guide rod;

[0040] When the test tube containing exosomes needs to be stored or taken out, the motor 4 fixedly installed at the bottom of the main body 1 drives the driving gear 6 fixed on the rotating shaft 13 to rotate, and the driven gear 11 is engaged to rotate. Since the driven gear 11 is slidably installed on the screw rod 7, the screw rod 7 will also rotate synchronously, so that the nut 10 threadedly installed on the screw rod 7 can be driven to move up and down;

[0041] When the nut 10 moves up and down, it will also drive the support part 2 fixedly connected to the nut 10 to move up and down. At the same time, the support part 2 moving up and down will be coordinated by the slider fixed on the support part 2 and the slide rail 8 fixedly connected to the inner surface of the main body 1, so as to realize the positioning and guiding of the up and down movement of the support part 2. The number of slide rails 8 corresponds to the number of support parts 2.

[0042] Reference Figure 2 , Figure 3 and Figure 5The driving mechanism includes a motor 4, an electromagnetic clutch 5, a driving gear 6, a driven gear 11, a screw 7 and a nut 10. The electromagnetic clutch 5 is fixedly connected to the output end of the motor 4. By using the electromagnetic clutch 5, the power output to the driving gear 6 can be cut off without stopping the motor 4, and the driven gear 11 required for meshing can be flexibly adjusted effectively, so that the required test tubes containing exosomes can be taken out or stored at the same time. The end of the electromagnetic clutch 5 away from the motor 4 is fixedly connected to the rotating shaft 13, and the top of the rotating shaft 13 is rotatably connected to the bottom of the refrigeration chamber 3;

[0043] A switching mechanism is provided on the screw rod 7, and the switching mechanism includes a slide groove 14 and an electric push rod 12. The electric push rod 12 is fixedly connected to the surface of the screw rod 7, and the slide groove 14 is opened on the surface of the screw rod 7. The telescopic end of the electric push rod 12 is fixedly connected to the bottom of the driven gear 11, and a limit block is fixedly connected to the driven gear 11. The limit block cooperates with the slide groove 14, and the telescopic end of the electric push rod 12 can be extended or contracted, thereby driving the driven gear 11 to move up and down, and whether the driven gear 11 is engaged with the driving gear 6 can be flexibly adjusted.

[0044] Reference Figure 1 and Figure 2 A reserved opening is provided at the top of the main body 1, and the reserved openings are arranged in a circular array with the axis of the main body 1 as the center. A cover body is connected to the top of the reserved opening by a thread, and the cover body is in sealing contact with the reserved opening, which can ensure that the support part 2 for positioning and storing the test tube containing exosomes can be smoothly extended out of the main body 1 or stored in the inside of the main body 1;

[0045] A sealing cover is installed on the top of the refrigerating chamber 3, and the sealing cover is in sealing contact with the top of the refrigerating chamber 3. A through hole is provided on the side surface of the refrigerating chamber 3. The number of through holes is multiple, and the multiple through holes are distributed in an array on the refrigerating chamber 3, which can effectively ensure that the cold air flow is transmitted to the outside of the refrigerating chamber 3, thereby achieving a refrigeration effect on the inside of the main body 1;

[0046] The inner top of the main body 1 is fixedly connected with a connecting piece, and the bottom of the connecting piece is fixedly connected with a positioning plate 9. The bottom of the positioning plate 9 is rotatably connected to the top of the screw rod 7. The positioning plate 9 is in a circular ring shape, and the bottom of the screw rod 7 is rotatably connected to the inner bottom of the main body 1, which can effectively improve the stability of the positioning of the screw rod 7. The number of screw rods 7 corresponds to the number of support parts 2 and the number of reserved openings.

[0047] A method for preserving exosomes, comprising the following steps:

[0048] Step 1: Put the refrigerated iron block into the refrigeration chamber 3 and pre-cool the interior of the main body 1 in advance;

[0049] Step 2: Use the driving mechanism to extend the support part 2 to the outside of the main body 1, then place the test tube containing the exosomes on the support part 2, and then the support part 2 descends to the inside of the main body 1. The test tube containing the exosomes needs to be added with a protective agent. In the process of adding the protective agent, a syringe is used to insert the protective agent into the rubber sealing plug that seals the test tube;

[0050] Step 3: When it is necessary to take out the test tube containing the designated exosomes, the corresponding driven gear 11 is controlled to mesh with the driving gear 6 , thereby controlling the support portion 2 to extend out of the main body 1 .

[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A storage device and storage method for exosomes, comprising a main body (1), characterized in that: A support portion (2) for positioning a test tube is installed inside the main body (1), a driving mechanism for driving the support portion (2) to move is installed inside the main body (1), a positioning mechanism for assisting in clamping the test tube is installed inside the support portion (2), and a refrigeration chamber (3) is fixedly connected to the main body (1); The positioning mechanism comprises an auxiliary support rod (204) for positioning the test tube and a limiting support rod (206) for limiting the position, and one end of the auxiliary support rod (204) is rotatably connected to a roller (205); The support portion (2) comprises a positioning sleeve (201), a connecting plate (202) and a supporting base (203), and the other end of the auxiliary support rod (204) is rotatably connected to the connecting plate (202).

2. The storage device and storage method for exosomes according to claim 1, characterized in that: The positioning mechanism further comprises a guide rail (207), a moving block (208), a spring (209), a support plate (2010), a pneumatic telescopic rod (2011) and a pulley (2012); the pulley (2012) is symmetrically connected to the moving block (208) for rotation; the moving block (208) is fixedly connected to the top of the spring (209); the support plate (2010) is fixedly connected to the bottom of the spring (209); and the pulley (2012) is slidably connected to the inside of the guide rail (207).

3. The storage device and storage method for exosomes according to claim 2, characterized in that: One end of the limiting support rod (206) is rotatably connected to the auxiliary support rod (204), and the other end of the limiting support rod (206) is rotatably connected to the moving block (208). The pneumatic telescopic rod (2011) is fixedly connected to the connecting plate (202), and the support plate (2010) is fixedly connected to the telescopic end of the pneumatic telescopic rod (2011).

4. The storage device and storage method for exosomes according to claim 1, characterized in that: The positioning sleeve (201) is fixedly connected to the top of the connecting plate (202), the supporting base (203) is fixedly connected to the bottom of the connecting plate (202), and the connecting plates (202) are distributed on the supporting base (203) in a circular array.

5. The storage device and storage method for exosomes according to claim 1, characterized in that: The driving mechanism comprises a motor (4), an electromagnetic clutch (5), a driving gear (6), a driven gear (11), a screw rod (7) and a nut (10); the motor (4) is fixedly connected to the inner bottom of the main body (1); the electromagnetic clutch (5) is fixedly connected to the output end of the motor (4); an end of the electromagnetic clutch (5) away from the motor (4) is fixedly connected to a rotating shaft (13); and the top of the rotating shaft (13) is rotatably connected to the bottom of the refrigerating chamber (3).

6. The storage device and storage method for exosomes according to claim 5, characterized in that: The driven gear (11) is slidably connected to the screw rod (7), the driving gear (6) is fixedly connected to the rotating shaft (13), the driving gear (6) and the driven gear (11) are meshed together, the nut (10) is connected to the screw rod (7) by threads, and the nut (10) is connected to the support part (2).

7. The storage device and storage method for exosomes according to claim 5, characterized in that: The screw rod (7) is provided with a switching mechanism, the switching mechanism comprising a slide groove (14) and an electric push rod (12), the electric push rod (12) is fixedly connected to the surface of the screw rod (7), the slide groove (14) is opened on the surface of the screw rod (7), the telescopic end of the electric push rod (12) is fixedly connected to the bottom of the driven gear (11), the driven gear (11) is fixedly connected to a limit block, and the limit block cooperates with the slide groove (14).

8. The storage device and storage method for exosomes according to claim 1, characterized in that: The inner surface of the main body (1) is fixedly connected with a slide rail (8), the support portion (2) is fixedly connected with a slider, the slider is slidably connected inside the slide rail (8), and the slider cooperates with the slide rail (8).

9. A method for preserving exosomes according to claim 1, characterized in that: The following steps are involved: Step 1: Put the refrigerated iron block into the refrigeration chamber 3 and pre-cool the interior of the main body 1 in advance; Step 2: Use the driving mechanism to extend the support part 2 to the outside of the main body 1, then place the test tube containing exosomes on the support part 2, and then the support part 2 descends to the inside of the main body 1; Step 3: When it is necessary to take out the test tube containing the exosomes, the corresponding driven gear 11 is controlled to mesh with the driving gear 6 , thereby controlling the support part 2 to extend out of the main body 1 .

10. A method for preserving exosomes according to claim 9, characterized in that: In the step 2, a protective agent needs to be added to the test tube containing the exosomes. During the process of adding the protective agent, the protective agent is added by inserting a syringe into the rubber sealing plug that seals the test tube.

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