PCR (Polymerase Chain Reaction) detection method and equipment, quantitative PCR instrument and electronic equipment

Abstract

The invention relates to the field of molecular biology, and discloses a PCR detection method, PCR detection equipment, a quantitative PCR instrument, electronic equipment and a computer readable storage medium. The PCR detection method comprises the following steps: respectively carrying out PCR detection on each temperature control device in response to a received detection instruction of the temperature control device; in the detection process, in response to the received new detection instruction, continuously performing PCR detection until all the detection instructions are executed, and obtaining a whole-process detection result of each temperature control device; and selecting a PCR detection result of each temperature control device from the whole-flow detection results of each temperature control device based on the instruction time of each temperature control device. Compared with the prior art, the PCR detection method, the PCR detection equipment, the quantitative PCR instrument, the electronic equipment and the computer readable storage medium provided by the embodiment of the invention have the advantages that the PCR detection cost can be reduced while the reliability of a PCR detection result can be improved.

Description

Technical Field

[0001] The present application relates to the field of molecular biology, and specifically, to a PCR detection method, a PCR detection device, a quantitative PCR instrument, an electronic device, and a computer-readable storage medium. Background Art

[0002] A quantitative PCR (Polymerase Chain Reaction) detector is an instrument that uses dyes or fluorescently labeled specific probes to mark and track PCR products and monitor the reaction process in real time. Combined with the corresponding software, the product can be qualitatively and quantitatively analyzed and the initial concentration of the sample template to be tested can be calculated. Dyes or fluorescent markers are added to the PCR reaction system, and the accumulation of dye or fluorescent marker signals is used to monitor the entire PCR process in real time.

[0003] Existing quantitative PCR instruments are basically equipped with one detection device for one temperature control device. When there are multiple temperature control devices, multiple detection devices are generally equipped, or a motion structure is added so that the detection device moves between multiple temperature control devices. However, configuring a separate detection device for each temperature control device will lead to a significant increase in the production cost of the quantitative PCR instrument; and the method of moving the detection device between multiple temperature control devices requires the motion structure to move at a high speed and stably, and its signal credibility is affected by the movement rate and stability of the motion structure, resulting in reduced signal reliability. Summary of the invention

[0004] The purpose of the present application is to provide a PCR detection method, a PCR detection device, a quantitative PCR instrument, an electronic device and a computer-readable storage medium, which can improve the reliability of PCR detection results while reducing the cost of PCR detection.

[0005] In the first aspect, an embodiment of the present application provides a PCR detection method, which is applied to a PCR detection device connected to at least two temperature control devices, and the PCR detection method includes: in response to receiving a detection instruction from the temperature control device, performing PCR detection on each of the temperature control devices respectively; during the detection process of the PCR detection, in response to receiving a new detection instruction, continuing the PCR detection until all the detection instructions are executed, and obtaining a full-process detection result of each of the temperature control devices; and selecting the PCR detection result of each of the temperature control devices from the full-process detection results of each of the temperature control devices based on the instruction time of each of the temperature control devices.

[0006] Compared with the related art, in the PCR detection method provided in the embodiment of the present application, when there is a detection demand, the temperature control device can send a detection instruction to the PCR detection device. When the PCR detection device receives the detection instruction sent by any one of the at least two temperature control devices, it directly starts to perform PCR detection on each temperature control device respectively. When a new detection instruction sent by any temperature control device is received, since each temperature control device has started PCR detection, the PCR detection process can be directly maintained, and the PCR detection can be continuously performed until all detection instructions are executed, that is, the PCR detection is continuously performed until the detection requirements of all temperature control devices that have sent detection instructions are met. After the PCR detection is completed, the PCR detection results of each temperature control device can be selected from the full-process detection results of each temperature control device based on the instruction time of each temperature control device, thereby realizing a single PCR detection device to monitor at least two temperature control devices at the same time. Reducing the number of PCR detection devices can reduce the PCR detection cost. At the same time, since the detection equipment does not need to be moved between multiple temperature control devices, the reliability of the PCR detection results can also be effectively improved.

[0007] In an optional embodiment, the PCR detection is performed on each of the temperature control devices separately, including: for each of the temperature control devices, a PCR detection is performed on each detection channel of the temperature control device in turn as a detection round, and multiple detection rounds are repeated until all the detection instructions are executed. Repeating multiple detection rounds can make it possible for the detection channels that are at the front of the detection order in the detection round of the temperature control device that sends the detection instruction later to be detected again in the new detection round, so as to more completely detect each temperature control device that sends the detection instruction and more accurately execute all the detection instructions.

[0008] In an optional embodiment, the PCR test is continued until all the test instructions are executed, including: obtaining all the test rounds required to be executed when the new test instruction is received, and judging whether the new test instruction can be executed in all the test rounds; if the new test instruction cannot be executed in all the test rounds, the total number of test rounds is increased. When a new test instruction is received from any temperature control device, it is judged whether all the test rounds currently required to be executed can execute the new test instruction, that is, it is judged whether all the test rounds currently required to be executed can complete the test of any temperature control device. When all the test rounds currently required to be executed cannot complete the test of any temperature control device, the total number of test rounds is increased, thereby ensuring the integrity of the test process of any temperature control device.

[0009] In an optional embodiment, the PCR detection method further includes: if the new detection instruction can be executed in all detection rounds, the total number of detection rounds is maintained. If all the detection rounds currently required to be executed can complete the detection instructions sent by any temperature control device, it means that the detection of any temperature control device can be completed without adding new detection rounds. At this time, keeping the total number of detection rounds unchanged can minimize the total number of detection rounds and improve detection efficiency.

[0010] In an optional embodiment, the PCR detection result of each temperature control device is selected from the full-process detection results of each temperature control device based on the instruction time of each temperature control device, including: for each temperature control device, obtaining the target detection round and target detection channel that are in progress when the detection instruction of the temperature control device is received, obtaining the first channel detection result of the detection channel after the target detection channel in the target detection round in the full-process detection result, obtaining the second channel detection result of the detection channel before the target detection channel in the next detection round of the target detection round in the full-process detection result, obtaining the third channel detection result of the target detection channel in the target detection round in the full-process detection result, and merging the first channel detection result, the second channel detection result, and the third channel detection result into the PCR detection result.

[0011] In an optional embodiment, the PCR test is continued until all the test instructions are executed, including: obtaining the current test channel that is performing the PCR test when the new test instruction is received, and continuing the PCR test until the PCR test is performed on the current test channel again. When a new test instruction is received, the PCR test is continued until the PCR test of the current test channel is completed again, and there is no need to repeat the test of the subsequent test channels of the current test channel, thereby reducing the total number of test channels for PCR test and improving the test efficiency.

[0012] In the second aspect, an embodiment of the present application provides a PCR detection device, comprising: a communication module, which is used to receive detection instructions from at least two temperature control devices; a PCR detection module, which is used to perform PCR detection on each of the temperature control devices separately when receiving the detection instructions, and during the detection process of the PCR detection, in response to receiving a new detection instruction, continue to perform the PCR detection until all the detection instructions are executed, and obtain the full-process detection results of each temperature control device; a result screening module, which is used to select the PCR detection results of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device.

[0013] Compared with the related art, in the PCR detection device provided in the embodiment of the present application, the temperature control device can send a detection instruction to the PCR detection device when there is a detection demand. When the communication module in the PCR detection device receives the detection instruction sent by any one of at least two temperature control devices, the PCR detection module directly starts to perform PCR detection on each temperature control device separately through the PCR detection module. During the detection process, when the temperature control device receives a new detection instruction sent by any temperature control device, since each temperature control device has started PCR detection, the PCR detection module can directly maintain the PCR detection process and continue to perform PCR detection until all detection instructions are executed, that is, continue to perform PCR detection until all detection requirements of all temperature control devices that have sent detection instructions are met. After the PCR detection is completed, the result screening module can select the PCR detection results of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device, thereby realizing that a single PCR detection device monitors at least two temperature control devices at the same time. Reducing the number of PCR detection devices can reduce the PCR detection cost. At the same time, since the detection equipment does not need to be moved between multiple temperature control devices, the reliability of the PCR detection results can also be effectively improved.

[0014] In a third aspect, an embodiment of the present application provides a quantitative PCR instrument, comprising: a PCR detection device as described above, and at least two temperature control devices communicatively connected to the PCR detection device.

[0015] Compared with the related art, since the quantitative PCR instrument provided in the embodiment of the present application includes the PCR detection device as mentioned above, and at least two temperature control devices communicatively connected to the PCR detection device, it has the same technical effect as the aforementioned PCR detection device.

[0016] In a fourth aspect, an embodiment of the present application provides an electronic device, comprising: at least one processor for connecting to a camera and at least two temperature control devices; and a memory communicatively connected to the at least one processor; wherein the memory stores instructions executable by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the PCR detection method as described above to control the camera to perform PCR detection on the at least two temperature control devices.

[0017] In a fifth aspect, an embodiment of the present application provides a computer-readable storage medium storing a computer program, wherein the computer program is executed by a processor to implement the aforementioned PCR detection method. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can also be obtained based on these drawings.

[0019] Figure 1 A schematic diagram of the structure of a quantitative PCR instrument provided in an embodiment of the present application;

[0020] Figure 2 This is a schematic diagram of the process of the PCR detection method provided in Example 1 of the present application;

[0021] Figure 3 A schematic diagram of the detection timing of a single temperature control device in the PCR detection method provided in Example 1 of the present application;

[0022] Figure 4 This is a schematic diagram of the detection timing of three temperature control devices in the PCR detection method provided in Example 1 of the present application;

[0023] Figure 5 A schematic diagram of the process of continuously performing PCR detection in the PCR detection method provided in Example 1 of the present application;

[0024] Figure 6 A schematic diagram of the timing of a single PCR detection process in the PCR detection method provided in Example 1 of the present application;

[0025] Figure 7 A schematic diagram of a process of continuously performing PCR detection in a PCR detection method provided in another embodiment of the present application;

[0026] Figure 8 A schematic diagram of the timing of a single PCR detection process in a PCR detection method provided in another embodiment of the present application;

[0027] Fig. 9 A schematic diagram of the process of selecting PCR detection results in the PCR detection method provided in Example 1 of the present application;

[0028] Fig.10 This is a schematic diagram of the structure of the PCR detection device provided in Example 2 of the present application;

[0029] Fig.11 This is a schematic diagram of the structure of the electronic device provided in Example 4 of the present application. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be described in detail below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0031] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application as claimed, but is merely representative of selected embodiments of the present application.

[0032] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0033] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.

[0034] It should be noted that, in the absence of conflict, the features in the embodiments of the present application may be combined with each other.

[0035] The first embodiment of the present application provides a PCR detection method, which is applied to the PCR detection device provided in one embodiment of the present application, such as Figure 1 As shown, the PCR detection device 100 provided in one embodiment of the present application can be connected to at least two temperature control devices 200, and PCR reactions can be performed in the at least two temperature control devices 200 respectively, and the PCR detection device can detect the PCR reaction process in the at least two temperature control devices 200. Specifically, in some embodiments of the present application, the PCR detection device 100 can include, for example, a filter wheel and a camera, and the PCR detection device 100 and the at least two temperature control devices 200 are connected via optical fibers. The optical signals sent by the at least two temperature control devices 200 via the optical fibers can be photographed and collected by the camera after passing through the filter wheel, thereby realizing the detection of the PCR reaction process of the at least two temperature control devices 200.

[0036] like Figure 2 As shown, the PCR detection method provided in this embodiment 1 may specifically include the following steps:

[0037] Step S101: in response to receiving a detection instruction from a temperature control device, performing PCR detection on each temperature control device respectively.

[0038] Specifically, in step S101, for the temperature control device, when the temperature control device has a detection requirement, a detection instruction can be sent to the PCR detection device, that is, the PCR detection device is informed of its own detection requirement. In response to receiving the detection instruction of the temperature control device, PCR detection is performed on each temperature control device separately, that is, after the PCR detection device receives the detection instruction of any temperature control device, it performs separate and independent PCR detection on all temperature control devices. Corresponding to the aforementioned PCR detection device 100, for example, it can include a filter wheel and a camera in the embodiment, and PCR detection is performed on each temperature control device separately, that is, taking pictures using the light signals sent to each temperature control device separately.

[0039] Specifically, in this step S101, since the PCR detection processes of various temperature control devices are independent of each other, the PCR detection process for each temperature control device can be specifically as follows: performing a PCR detection on each detection channel of the temperature control device in turn as one detection round, and repeating multiple detection rounds until all detection instructions are executed. That is, for each temperature control device, it includes a number of detection channels. During the PCR detection process, PCR detection is performed on each detection channel in turn, and performing a PCR detection on all detection channels once is a detection round. After a detection round is completed, a new detection round is repeated until all detection instructions are executed. Specifically, as Figure 3 As shown in the figure, taking four-channel detection as an example, each temperature control device has four detection channels. Figure 3 In the timing diagram shown, PCR detection starts at timing 4, and the PCR detection process of a detection channel is completed in each timing, then timing 4-7 is a detection round, timing 8-11 is a detection round... until all detection instructions are executed. Repeating multiple detection rounds can make the detection channels that are ahead of the detection order in the detection round of the temperature control device that sends the detection instruction later be detected again in the new detection round, so as to more completely detect each temperature control device that sends the detection instruction and more accurately execute all detection instructions.

[0040] Step S102: During the PCR test, in response to receiving a new test instruction, the PCR test is continued until all test instructions are executed, and the full-process test results of each temperature control device are obtained.

[0041] In this step, during the PCR detection process, in response to receiving a new detection instruction, that is, receiving a new detection instruction sent by any temperature control device during the time period when PCR detection of each temperature control device has started and has not yet been completed, PCR detection of each temperature control device will continue to be performed until all detection instructions are executed.

[0042] Specifically, in this step, if Figure 4 As shown, taking three temperature control devices as an example, when the detection time period of temperature control device 1 starts, the detection time periods of temperature control devices 2 and 3 have not yet arrived, and when the detection time period of temperature control device 2 starts, the detection time period of temperature control device 1 has not yet ended, and the detection time period of temperature control device 3 has not yet arrived..., therefore, when the detection time period of temperature control device 1 starts and a detection instruction is sent to the detection device, according to the relevant records in step S101, PCR detection of temperature control devices 1, 2, and 3 has begun at this time; and when the detection time period of temperature control device 2 starts and a detection instruction is sent to the detection device, the detection time period of temperature control device 1 has not yet ended, that is, the PCR detection process is still ongoing, and this is the case in step S102 where a new detection instruction is received during the detection process of PCR detection; and when the detection time period of temperature control device 3 starts and a detection instruction is sent to the detection device, the detection time periods of temperature control devices 1 and 2 have not yet ended, that is, the PCR detection process is still ongoing, and this is also the case in step S102 where a new detection instruction is received during the detection process of PCR detection.

[0043] Specifically, in this embodiment, if Figure 5 As shown, continuing the PCR test until all the test instructions are executed may specifically include the following steps:

[0044] Step S201: Obtain all detection rounds that need to be executed when a new detection instruction is received.

[0045] Step S202: Determine whether the new detection instructions in all detection rounds can be executed. If not, execute step S203; if so, execute step S204.

[0046] Step S203: Increase the total number of detection rounds.

[0047] Step S204: Maintain the total number of detection rounds.

[0048] In step S201, all detection rounds are all detection rounds that have been accumulated and increased by step S203 when a new detection instruction is received.

[0049] In step S202, judging whether the new detection instruction can be executed in all detection rounds is to judge whether the remaining detection process is sufficient to perform a PCR test on all detection channels of the temperature control device corresponding to the new detection instruction. For example, when a new detection instruction is received for the first time, the detection round has not been increased by step S203, and the corresponding total number of detection rounds is 1; when a new detection instruction is received for the second time, if the detection round has been increased by step S203, the corresponding total number of detection rounds is 2...

[0050] like Figure 6 As shown, taking three temperature control devices and four-channel detection as an example, for temperature control device 1, the detection instruction of temperature control device 1 is executed at timing 4 and detection channel A is detected, and detection channels B, C, and D are detected in subsequent timings 5-7 respectively; for temperature control device 2, as in the technical solution recorded in step S101, although the detection of temperature control device 2 has begun at timing 4, the detection instruction of temperature control device 2 has not been received, and the detection instruction of temperature control device 2 is executed at timing 5 and detection channel B is detected, and detection channels C, D, and A are detected in subsequent timings 6-8 respectively; for temperature control device 3, as in the technical solution recorded in step S101, although the detection of temperature control device 3 has begun at timing 4, the detection instruction of temperature control device 3 has not been received, and the detection instruction of temperature control device 3 is executed at timing 6 and detection channel C is detected, and detection channels D, A, and B are detected in subsequent timings 7-9 respectively. Among them, when a new detection instruction sent by the temperature control device 2 is received at time sequence 5, it corresponds to step S201. At this time, the total number of detection rounds to be executed is 1. At this time, it corresponds to step S202, that is, it is determined whether the detection instruction sent by the temperature control device 2 can be executed within the detection round of time sequence 4-7. Since the detection instruction sent by the temperature control device 2 needs to be completed at least within time sequence 5-8, it is determined that the detection instruction sent by the temperature control device 2 cannot be executed within the detection round of time sequence 4-7. At this time, step S203 is executed; when receiving at time sequence 6, When a new detection instruction is sent by the temperature control device 3, it corresponds to step S201. At this time, the total number of detection rounds to be executed is 2, which corresponds to step S202, that is, it is determined whether the detection instruction sent by the temperature control device 3 can be completed within the two detection rounds of timing 4-7 and timing 8-11. Since the detection instruction sent by the temperature control device 3 needs to be completed at least within timing 6-9, it is determined that the detection instruction sent by the temperature control device 3 can be completed within the two detection rounds of timing 4-7 and timing 8-11, and step S204 is executed at this time.

[0051] When receiving a new detection instruction sent by a new temperature control device, determine whether all the detection rounds currently required to be executed can execute the new detection instruction, that is, determine whether all the detection rounds currently required to be executed can complete the detection of the new temperature control device. When all the detection rounds currently required to be executed cannot complete the detection of the new temperature control device, increase the total number of detection rounds to ensure the integrity of the detection process of the new temperature control device; and if all the detection rounds currently required to be executed can complete the detection instruction sent by the new temperature control device, it means that the detection of the new temperature control device can be completed without adding new detection rounds. At this time, keeping the total number of detection rounds unchanged can minimize the total number of detection rounds and improve detection efficiency.

[0052] It is understandable that the above Figure 5 The technical solution shown is only an example of a specific implementation method of continuously performing PCR detection until all detection instructions are executed in this embodiment 1. In some other embodiments of the present application, for example, it can also be as follows Figure 7 As shown, the following steps are included:

[0053] Step S301: Acquire the current detection channel that is performing PCR detection when a new detection instruction is received.

[0054] Step S302: Continue to perform PCR detection until the current detection channel is subjected to PCR detection again.

[0055] Still taking three temperature control devices and four-channel detection as an example, Figure 8 As shown, for temperature control device 1, the detection instruction of temperature control device 1 is executed at timing 4, and detection channel A is detected. In subsequent timings 5-7, detection channels B, C, and D are detected respectively; for temperature control device 2, as in the technical solution recorded in step S101, although the detection of temperature control device 2 has been started at timing 4, the detection instruction of temperature control device 2 has not been received. The detection instruction of temperature control device 2 is executed at timing 5 and detection channel B is detected. In subsequent timings 6-8, detection channels C, D, and A are detected respectively; for temperature control device 3, as in the technical solution recorded in step S101, although the detection of temperature control device 3 has been started at timing 4, the detection instruction of temperature control device 3 has not been received. The detection instruction of temperature control device 3 is executed at timing 6 and detection channel C is detected. In subsequent timings 7-9, detection channels D, A, and B are detected respectively. Among them, when a new detection instruction sent by the temperature control device 2 is received at timing 5, corresponding to step S301, the current detection channel B that is performing PCR detection when the new detection instruction is received is obtained, corresponding to step S302, that is, the PCR detection is continuously performed until the current detection channel B is PCR detected again at timing 8; when a new detection instruction sent by the temperature control device 3 is received at timing 6, corresponding to step S301, the current detection channel C that is performing PCR detection when the new detection instruction is received is obtained, corresponding to step S302, that is, the PCR detection is continuously performed until the current detection channel C is PCR detected again at timing 9.

[0056] When a new detection instruction is received, the PCR detection is continued until the PCR detection of the current detection channel is completed again. Figure 5 In the technical solution shown, the detection time is extended by increasing the number of detection rounds, and there is no need to repeat the detection of subsequent detection channels of the current detection channel, thereby reducing the total number of detection channels for PCR detection and improving detection efficiency.

[0057] In this step, starting from the PCR test of each temperature control device in step S101 and ending with the completion of all test instructions, all test results of each test device during the entire PCR test process are the full-process test results of the test device. Figure 6 As shown, the full-process detection results corresponding to temperature control devices 1, 2, and 3 are all the detection results of time sequences 4-11.

[0058] Step S103: Selecting the PCR detection result of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device.

[0059] In this step, if Fig. 9 As shown, selecting the PCR test result may specifically include the following steps:

[0060] Step S401: Acquire the target detection round and target detection channel that are being performed when receiving the detection instruction of the temperature control device.

[0061] like Figure 6 As shown, for temperature control device 1, the target detection round in progress when the detection instruction of the temperature control device is received is 1, and the target detection channel is detection channel A; for temperature control device 2, the target detection round in progress when the detection instruction of the temperature control device is received is 1, and the target detection channel is detection channel B; for temperature control device 3, the target detection round in progress when the detection instruction of the temperature control device is received is 1, and the target detection channel is detection channel C.

[0062] Step S402: Obtain the first channel detection result of the detection channel after the target detection channel in the target detection round in the full process detection result.

[0063] like Figure 6 As shown, for temperature control device 1, the target detection round is 1, and the target detection channel is detection channel A. At this time, the target detection round is 1, and the detection channels B, C, and D after the target detection channel A are obtained in the first channel detection result of the whole process detection result; for temperature control device 2, the target detection round is 1, and the target detection channel is detection channel B. At this time, the target detection round is 1, and the target detection channel is detection channel B. At this time, the target detection round is 1, and the detection channels C and D after the target detection channel B are obtained in the first channel detection result of the whole process detection result; for temperature control device 3, the target detection round is 1, and the target detection channel is detection channel C. At this time, the target detection round is 1, and the detection channel D after the target detection channel C is obtained in the first channel detection result of the whole process detection result.

[0064] Step S403: obtaining the second channel detection result of the detection channel before the target detection channel in the next detection round of the target detection round in the full-process detection result.

[0065] like Figure 6 As shown, for temperature control device 1, the target detection round is 1, and the target detection channel is detection channel A. At this time, in the next detection round 2, there is no detection channel before target detection channel A, and the second channel detection result corresponding to temperature control device 1 is empty; for temperature control device 2, the target detection round is 1, and the target detection channel is detection channel B. At this time, in the next detection round 2, the second channel detection result of detection channel A before target detection channel B in the whole process detection result is obtained; for temperature control device 3, the target detection round is 1, and the target detection channel is detection channel C. At this time, in the next detection round 2, the second channel detection results of detection channels A and B before target detection channel C in the whole process detection result are obtained.

[0066] Step S404: Obtain the third channel detection result of the target detection channel in the target detection round in the full process detection result.

[0067] like Figure 6 As shown, for temperature control device 1, the target detection round is 1, and the target detection channel is detection channel A. At this time, the third channel detection result of target detection channel A in target detection round 1 in the whole process detection result is obtained; for temperature control device 2, the target detection round is 1, and the target detection channel is detection channel B. At this time, the third channel detection result of target detection channel B in target detection round 1 in the whole process detection result is obtained; for temperature control device 3, the target detection round is 1, and the target detection channel is detection channel C. At this time, the third channel detection result of target detection channel C in target detection round 1 in the whole process detection result is obtained.

[0068] Step S405: merging the first channel detection result, the second channel detection result, and the third channel detection result into a PCR detection result.

[0069] The following table shows the test results corresponding to the temperature control device 3. Each cycle number corresponds to Figure 4 In the detection time period shown, starting from the 26th cycle, there are multiple temperature control devices that need to be detected, and two rounds of detection are required.

[0070]

[0071] Correspondingly, as shown in the following table, the data of channels C and D in detection round 1 and the data of channels A and B in detection round 2 can be selected as the PCR detection results of the temperature control device 3.

[0072]

[0073] Compared with the related art, in the PCR detection method provided in the first embodiment of the present invention, when there is a detection demand, the temperature control device can send a detection instruction to the PCR detection device. When the PCR detection device receives the detection instruction sent by any one of the at least two temperature control devices, it directly starts to perform PCR detection on each temperature control device respectively. When a new detection instruction sent by any temperature control device is received, since each temperature control device has started PCR detection, the PCR detection process can be directly maintained, and the PCR detection can be continuously performed until all detection instructions are executed, that is, the PCR detection is continuously performed until the detection requirements of all temperature control devices that have sent detection instructions are met. After the PCR detection is completed, the PCR detection results of each temperature control device can be selected from the full-process detection results of each temperature control device based on the instruction time of each temperature control device, thereby realizing that a single PCR detection device monitors at least two temperature control devices at the same time. Reducing the number of PCR detection devices can reduce the PCR detection cost. At the same time, since the detection equipment does not need to be moved between multiple temperature control devices, the reliability of the PCR detection results can also be effectively improved.

[0074] Embodiment 2

[0075] Embodiment 2 of the present application provides a PCR detection device 100, such as Figure 1 As shown, the PCR detection device 100 can be connected to at least two temperature control devices 200. Fig.10 As shown, the PCR detection device 100 may specifically include: a communication module 101, the communication module 101 is used to receive detection instructions from at least two temperature control devices; a PCR detection module 102, the PCR detection module 102 is used to perform PCR detection on each temperature control device respectively when receiving the detection instruction, and in the detection process of the PCR detection, in response to receiving a new detection instruction, continue to perform PCR detection until all detection instructions are executed, and obtain the full-process detection results of each temperature control device; a result screening module 103, the result screening module 103 is used to select the PCR detection results of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device.

[0076] Among them, the PCR detection module 102 can be composed of a filter wheel and a camera, for example. The PCR detection module 102 is connected to at least two temperature control devices via optical fiber. The optical signals sent by the at least two temperature control devices via the optical fiber can be photographed and collected by the camera after passing through the filter wheel, thereby realizing the detection of the PCR reaction process of the at least two temperature control devices.

[0077] Compared with the related art, in the PCR detection device provided in the embodiment of the present application, the temperature control device can send a detection instruction to the PCR detection device when there is a detection demand. When the communication module 101 in the PCR detection device receives the detection instruction sent by any one of at least two temperature control devices, the PCR detection module 102 directly starts to perform PCR detection on each temperature control device. During the detection process, when the temperature control device receives a new detection instruction sent by a new temperature control device, since each temperature control device has started PCR detection, the PCR detection module 102 can directly maintain the PCR detection process and continue to perform PCR detection until all detection instructions are executed, that is, continue to perform PCR detection until all detection requirements of all temperature control devices that have sent detection instructions are met. After the PCR detection is completed, the result screening module 103 can select the PCR detection results of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device, thereby realizing that a single PCR detection device monitors at least two temperature control devices at the same time. Reducing the number of PCR detection devices can reduce the PCR detection cost. At the same time, since the detection device does not need to be moved between multiple temperature control devices, the reliability of the PCR detection results can also be effectively improved.

[0078] Embodiment 3

[0079] Embodiment 3 of the present application provides a quantitative PCR instrument, such as Figure 1 As shown, it includes: a PCR detection device 100 as provided in the above embodiment, and at least two temperature control devices 200 communicatively connected to the PCR detection device 100.

[0080] Compared with the related art, since the quantitative PCR instrument provided in the embodiment of the present application includes the PCR detection device provided in the previous embodiment, and at least two temperature control devices communicatively connected to the PCR detection device, it has the same technical effect as the PCR detection device provided in the previous embodiment.

[0081] Embodiment 4

[0082] Embodiment 4 of the present application relates to an electronic device, such as Fig.11 As shown, it includes: at least one processor 111, which can be connected to a camera and at least two temperature control devices; and a memory 112 that is communicatively connected to the at least one processor 111; wherein the memory 112 stores instructions that can be executed by the at least one processor 111, and the instructions are executed by the at least one processor 111 so that the at least one processor 111 can execute the methods in the above-mentioned embodiments to control the camera to perform PCR detection on at least two temperature control devices.

[0083] Specifically, the processor can respond to receiving a detection instruction from the temperature control device and control the camera to perform PCR detection on each temperature control device respectively; during the PCR detection process, the processor can also respond to receiving a new detection instruction and control the camera to continue to perform PCR detection until all detection instructions are executed, thereby obtaining the full-process detection results of each temperature control device; the processor can also select the PCR detection results of each temperature control device from the full-process detection results of each temperature control device based on the instruction time of each temperature control device.

[0084] Among them, the memory and the processor are connected in a bus manner, and the bus may include any number of interconnected buses and bridges, and the bus connects various circuits of one or more processors and memories together. The bus can also connect various other circuits such as peripherals, voltage regulators, and power management circuits, which are well known in the art and are therefore not further described herein. The bus interface provides an interface between the bus and the transceiver. The transceiver can be one element or multiple elements, such as multiple receivers and transmitters, providing a unit for communicating with various other devices on a transmission medium. The data processed by the processor is transmitted on a wireless medium via an antenna, and further, the antenna also receives data and transmits the data to the processor.

[0085] The processor is responsible for managing the bus and general processing, and can also provide various functions, including timing, peripheral interfaces, voltage regulation, power management, and other control functions. Memory can be used to store data used by the processor when performing operations.

[0086] Embodiment 5

[0087] Embodiment 5 of the present application relates to a computer-readable storage medium storing a computer program. When the computer program is executed by a processor, the above method embodiment is implemented.

[0088] That is, those skilled in the art can understand that all or part of the steps in the above-mentioned embodiment method can be completed by instructing the relevant hardware through a program, and the program is stored in a storage medium, including several instructions to enable a device (which can be a single-chip microcomputer, chip, etc.) or a processor to execute all or part of the steps of each embodiment method of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk and other media that can store program codes.

[0089] The above are only specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A PCR detection method, applied to a PCR detection device connected to at least two temperature control devices, characterized in that: The PCR detection method comprises: In response to receiving a detection instruction from the temperature control device, performing PCR detection on each of the temperature control devices; During the detection process of the PCR detection, in response to receiving a new detection instruction, the PCR detection is continuously performed until all the detection instructions are executed, and the full-process detection results of each of the temperature control devices are obtained; The PCR detection result of each temperature control device is selected from the full-process detection results of each temperature control device based on the instruction time of each temperature control device.

2. The PCR detection method according to claim 1, characterized in that The PCR detection is performed on each of the temperature control devices respectively, including: For each of the temperature control devices, a PCR test is performed on each detection channel of the temperature control device in turn as one detection round, and multiple detection rounds are repeated until all the detection instructions are executed.

3. The PCR detection method according to claim 2, characterized in that: The step of continuing the PCR test until all the test instructions are executed includes: Obtaining all detection rounds required to be executed when the new detection instruction is received, and determining whether the new detection instruction can be executed within all detection rounds; If the new detection instruction cannot be executed completely within all detection rounds, the total number of detection rounds is increased.

4. The PCR detection method according to claim 3, characterized in that: The PCR detection method further comprises: If the new detection instruction can be executed completely within all detection rounds, the total number of detection rounds is maintained.

5. The PCR detection method according to claim 2, characterized in that: The selecting the PCR test result of each temperature control device from the full-process test results of each temperature control device based on the instruction time of each temperature control device includes: For each of the temperature control devices, obtain the target detection round and target detection channel that are in progress when the detection instruction of the temperature control device is received, obtain the first channel detection result of the detection channel after the target detection channel in the target detection round in the full-process detection result, obtain the second channel detection result of the detection channel before the target detection channel in the next detection round of the target detection round in the full-process detection result, obtain the third channel detection result of the target detection channel in the target detection round in the full-process detection result, and merge the first channel detection result, the second channel detection result, and the third channel detection result into the PCR detection result.

6. The PCR detection method according to claim 2, characterized in that: The step of continuing the PCR test until all the test instructions are executed includes: The current detection channel that is performing PCR detection when the new detection instruction is received is obtained, and the PCR detection is continued until the PCR detection is performed on the current detection channel again.

7. A PCR detection device, characterized in that: include: A communication module, the communication module is used to receive detection instructions from at least two temperature control devices; A PCR detection module, wherein the PCR detection module is used to perform PCR detection on each of the temperature control devices respectively when receiving the detection instruction. During the detection process of the PCR detection, in response to receiving a new detection instruction, the PCR detection is continuously performed until all the detection instructions are executed, thereby obtaining the full-process detection results of each of the temperature control devices; A result screening module is used to select the PCR test results of each temperature control device from the full-process test results of each temperature control device based on the instruction time of each temperature control device.

8. A quantitative PCR instrument, characterized in that: include: The PCR detection device as described in claim 7, and at least two temperature control devices communicatively connected to the PCR detection device.

9. An electronic device, characterized in that: include: at least one processor for interfacing with the camera and the at least two temperature control devices; and, a memory communicatively coupled to the at least one processor; Wherein, the memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the PCR detection method as described in any one of claims 1 to 6 to control the camera to perform PCR detection on the at least two temperature control devices.

10. A computer-readable storage medium storing a computer program, characterized in that: The computer program is executed by a processor to implement the PCR detection method according to any one of claims 1 to 6.

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