Air bath hatching method for horseshoe crab eggs

Through the air bath incubation method, a multi-layer incubation rack and automatic control system are used to solve the problems of insufficient dissolved oxygen, difficulty in temperature control and water quality pollution in the incubation in water, and an efficient and safe horseshoe egg hatching process is achieved.

CN119422964APending Publication Date: 2025-02-14BEIBU GULF UNIV
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Patent Information

Application Number
CN202411743818.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-30
Publication Date
2025-02-14

AI Technical Summary

Technical Problem

In the incubation method, there are problems such as insufficient dissolved oxygen, difficulty in temperature control and water quality pollution.

Method used

The air bath incubation method is adopted, by building a multi-layer incubation rack, setting up a water sprinkler pipe and a temperature control system, maintaining appropriate humidity and temperature, ensuring that the horseshoe horse eggs are hatched in a stable air environment.

Benefits of technology

Accurate control of the incubation environment is achieved, the success rate of incubation is improved, energy and maintenance costs are reduced, and the risk of microbial pollution is reduced.

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Abstract

The invention relates to the technical field of biological engineering and aquaculture, and discloses a horseshoe crab egg air bath incubation method which comprises the following steps: S1, constructing a multi-layer incubation frame, arranging incubation basins on each layer, slightly inclining the incubation basins, forming openings in the bottoms of the lower ends of the incubation basins, connecting drainage pipes for draining water, and mounting water spray pipes above the incubation basins; s2, a net disc is placed in the hatching basin, a water-absorbing non-woven fabric is placed on the net disc, and horseshoe crab eggs are evenly distributed on the wet non-woven fabric; s3, continuously or alternately spraying the hatching basins on each layer of the hatching frame through a continuous spraying system, and maintaining proper humidity. Through the independent temperature and humidity regulation and control units, the accuracy and response speed of environment control are improved, waste of energy and water resources is effectively reduced through an automatic temperature and humidity management system, sanitation and safety of incubation equipment are improved through cleaning and disinfection measures, the pollution risk of pathogens is reduced, and therefore the incubation efficiency and the success rate are improved.
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Description

Technical Field

[0001] The invention relates to the technical field of bioengineering and aquaculture, and in particular to an air bath hatching method for horseshoe crab eggs. Background Art

[0002] The air bath incubation method for horseshoe crab eggs is a technology that incubates horseshoe crab eggs in an environment mainly composed of air. Compared with the traditional incubation method in water, this method mainly relies on controlling the humidity and temperature in the air to promote the hatching of eggs. This technology simulates the incubation conditions of horseshoe crab eggs in the natural environment, uses a continuous spray system to maintain the necessary humidity, and adjusts the ambient temperature through a temperature control system to support the healthy development of horseshoe crab eggs.

[0003] Traditionally, horseshoe crab eggs are incubated in water, and the incubation environment usually depends on water temperature and dissolved oxygen levels. To improve the efficiency of incubation, net baskets are usually added to the surface of the water to keep the horseshoe crab eggs in a high dissolved oxygen area, and water quality is maintained by aerating or changing the water.

[0004] However, it is difficult to control water temperature, and the continuous water change process is often accompanied by high energy and equipment maintenance costs. In addition, the water quality can easily be polluted if not handled with care, leading to obstructed embryonic development or hatching failure. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides an air bath incubation method for horseshoe crab eggs, which solves the problems of insufficient dissolved oxygen, difficult temperature control and water pollution in incubation in water.

[0006] To achieve the above object, the present invention is implemented by the following technical scheme: an air bath incubation method for horseshoe crab eggs, comprising the following steps:

[0007] S1. Construct a multi-layer hatching rack, with shallow hatching basins on each layer. The hatching basins are slightly tilted, with an opening at the bottom of the lower end connected to a drain pipe for drainage. A water spray pipe is installed above the hatching basin and fixed on the hatching rack;

[0008] S2. A mesh tray is set in the incubation basin, a water-absorbing non-woven fabric is placed on the mesh tray, and horseshoe crab eggs are evenly distributed on the moist non-woven fabric;

[0009] S3. Use the spray system to spray each layer of the hatching rack continuously or intermittently to maintain appropriate humidity;

[0010] S4. Regulate the indoor temperature through the temperature control system to maintain a stable incubation environment;

[0011] S5. Continue to spray and monitor temperature and humidity until hatching is complete.

[0012] Preferably, the S1 comprises the following steps:

[0013] The hatching rack is made of corrosion-resistant materials, preferably stainless steel or anti-corrosion treated wood;

[0014] The bottom of the incubation basin is slightly tilted to one side, and a drain port is provided at the bottom of the basin to facilitate the discharge of excess water.

[0015] Preferably, S2 comprises the following steps:

[0016] The net plate is composed of a frame and a sieve silk net. The frame contains a number of pillars, which support the net plate about 5 cm from the bottom. The sieve silk net can be made of a nylon net with a mesh size of about 20, which can effectively support horseshoe crab eggs and non-woven fabrics.

[0017] The non-woven fabric is a highly absorbent material and has the strength to maintain stability when wet, thus preventing the displacement of horseshoe crab eggs.

[0018] Preferably, S3 comprises the following steps:

[0019] The spray system uses an automatic control device to adjust the intensity and frequency of the spray according to the actual humidity. The water source is filtered, pollution-free clean seawater.

[0020] The nozzles are evenly distributed on the top of each layer of hatching racks, and the water droplets sprayed are small and uniform.

[0021] Preferably, S4 comprises the following steps:

[0022] The indoor temperature control system includes electronic temperature control devices, air conditioners, etc., which can adjust the temperature of the incubation environment in real time;

[0023] The temperature control system can monitor the temperature of each layer through sensors and automatically adjust it to the set temperature range.

[0024] Preferably, S3 further comprises:

[0025] The spray frequency range is set to 1 to 3 times per minute, and the continuous spray duration is adjusted between 10 seconds and 30 seconds per time according to the humidity conditions to ensure that the humidity of the hatching environment is always maintained between 60% and 80%.

[0026] Preferably, S5 includes:

[0027] The monitoring steps include visual inspection of horseshoe crab eggs for color and morphological changes, examination of embryonic development using a stereo microscope, and combined with sensors to monitor temperature and humidity parameters;

[0028] When hatching abnormalities are detected, timely adjust the continuous spray intensity, frequency and indoor temperature to ensure that horseshoe crab eggs are hatched under optimal conditions.

[0029] Preferably, the method further comprises:

[0030] After hatching is completed, clean and disinfect the net tray, hatching basin and hatching rack;

[0031] Cleaning steps: Use fresh water to clean the hatching basin and mesh plate to remove surface residues;

[0032] The disinfection step uses disinfectants, sanitizers or ultraviolet disinfection devices to thoroughly disinfect the area to ensure that no pathogens or harmful substances remain during the next batch of incubation;

[0033] Once cleaning and disinfection is complete, the equipment is dried and reassembled, ready for the next batch of hatching operations.

[0034] Preferably, the S4 further comprises:

[0035] Each layer of the hatching rack is equipped with an independent humidity control unit to ensure that the hatching conditions of each layer can be adjusted separately to meet the needs of horseshoe crab eggs in different batches or horseshoe crab eggs in different developmental stages in the same batch;

[0036] Each temperature and humidity control unit is connected through a central control system, supporting batch adjustment and layered independent control.

[0037] Preferably, the continuous spray system is linked to the central air-conditioning system via a temperature sensor, and when it is detected that the incubation environment temperature exceeds a preset upper limit, the system automatically starts a temperature adjustment mode.

[0038] The present invention provides an air bath incubation method for horseshoe crab eggs. The method has the following beneficial effects:

[0039] 1. The present invention solves the limitation of dissolved oxygen in water and realizes precise management of temperature and humidity of hatching environment by linking independent humidity control units on each floor with central air conditioning control system. This automated control system can adjust hatching conditions in real time to ensure that horseshoe crab eggs at each developmental stage are hatched in the most suitable environment, thereby improving hatching success rate.

[0040] 2. The present invention adopts a method of dynamically adjusting the frequency and duration of continuous spraying. The system not only responds quickly, but also enhances the economic benefits of the entire hatching process by optimizing the use of water resources and energy. This intelligent regulation reduces energy waste and ensures the rapid stabilization of environmental conditions.

[0041] 3. The present invention uses high-temperature steam disinfection and ultraviolet sterilization technology to thoroughly clean and disinfect the incubation equipment, greatly reducing the risk of microbial contamination. The thorough disinfection of the equipment ensures the sanitation and safety of the incubation environment, avoids the spread of pathogens, and provides a healthier growth environment for horseshoe crab eggs. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 The present invention is a flow chart of the method. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Please see attached Figure 1 The embodiment of the present invention provides an air bath incubation method for horseshoe crab eggs, comprising the following steps:

[0045] S1. Construct a multi-layer hatching rack with a spacing of more than 20 cm between each layer. Each layer is equipped with a hatching basin. The hatching basin is slightly tilted and has an opening at the bottom of the lower end. A drainage pipe is connected to it for drainage. A water spray pipe is installed above the hatching basin. The water spray pipe provides a stable bearing surface, which is not only convenient for keeping the environment clean during hatching, but also helps to evenly distribute water, effectively improving the success rate of hatching;

[0046] S2. Place a mesh plate in the incubation basin, 5 to 10 cm away from the bottom of the incubation basin, place a water-absorbing non-woven fabric on the mesh plate, and evenly distribute the horseshoe crab eggs on the moist non-woven fabric. The water-absorbing non-woven fabric can not only continuously maintain the humidity required for incubation, but also avoid the risk of friction or damage caused by direct contact between the horseshoe crab eggs and the mesh plate. The evenly distributed horseshoe crab eggs ensure that they can receive appropriate humidity and oxygen supply, reducing the possibility of hatching failure.

[0047] S3. Continuously or intermittently spray each layer of the hatching rack through a continuous spray system to maintain appropriate humidity. Through the application of the automatic continuous spray system, the system can respond to humidity changes in a timely manner to ensure that the hatching environment is always at the optimal humidity level. Automatic adjustment of the continuous spray frequency and intensity can reduce human intervention and reduce operational complexity, while ensuring the success rate and efficiency of horseshoe crab egg hatching;

[0048] S4. A heated air conditioning control system is installed indoors to adjust the indoor temperature in 0.5°C intervals. The temperature control system is linked with the continuous spray system to achieve automated temperature management, ensuring that the ambient temperature is stable and does not fluctuate, avoiding the impact of excessively high or low temperatures on the hatching rate, and further improving the hatching success rate.

[0049] S5. Continuously or intermittently spray and monitor the temperature and humidity until the hatching is completed. Through continuous spraying and precise temperature and humidity monitoring, the entire hatching process can be operated unmanned, greatly reducing the frequency of human intervention. At the same time, real-time monitoring and automatic adjustment functions ensure the stability of the hatching environment and improve the safety and success rate of the hatching process.

[0050] Please see attached Figure 1 In a preferred embodiment of the present invention, S1 comprises the following steps:

[0051] The hatching rack is made of corrosion-resistant materials, preferably stainless steel or anti-corrosion treated wood. The hatching rack is placed indoors. The use of corrosion-resistant materials not only makes the hatching rack have good corrosion resistance and strength, but also can bear the weight of the multi-layer structure, so that it will not be damaged by corrosion when used for a long time, reducing the frequency of maintenance and replacement;

[0052] The bottom of the incubation basin is slightly tilted to one side, and a drain port is set at the bottom of the basin to facilitate the discharge of excess water. A water spray pipe is arranged above the incubation basin so that the water can cover the incubation basin. The tilted design of the bottom of the incubation basin and the configuration of the drain port can effectively prevent water from accumulating during the incubation process. Timely discharge of water can maintain the appropriate humidity of the incubation environment and avoid affecting the incubation process due to excessive water accumulation. At the same time, this design improves the stability of the environment and reduces the maintenance requirements of the equipment.

[0053] Please see attached Figure 1 In a preferred embodiment of the present invention, S2 comprises the following steps:

[0054] The mesh plate is composed of a frame and a sieve mesh. The sieve mesh is a nylon mesh with a mesh size of about 20, which can effectively support horseshoe crab eggs and non-woven fabrics. By using fine and durable materials, it can prevent horseshoe crab eggs from passing through the mesh due to their small size, while allowing air and water mist to flow freely to maintain a moist environment. In addition, the surface of the screen should be treated to be relatively smooth to avoid physical damage to horseshoe crab eggs or non-woven fabrics, thereby reducing maintenance costs and frequency, while ensuring a stable environment during the incubation process and improving the hatching success rate;

[0055] Non-woven fabric is a highly absorbent material with the ability to maintain stable strength when wet, preventing horseshoe crab eggs from shifting. Non-woven fabric can still maintain a high physical strength when wet and will not break or deform excessively due to water absorption, thereby protecting horseshoe crab eggs from being compressed or shifted during the incubation process. The use of non-woven fabric ensures that the position of horseshoe crab eggs is fixed during the incubation process, reducing the risk of hatching failure due to displacement. In addition, the easy-to-replace feature also simplifies the cleaning and preparation work after hatching, and improves the efficiency and sanitary conditions of the incubation process.

[0056] Please see attached Figure 1 In a preferred embodiment of the present invention, S3 includes the following steps:

[0057] The continuous spraying system uses an automatic control device to adjust the intensity and frequency of continuous spraying according to the actual humidity. Through automatic control, when the humidity is lower than the set threshold, the controller activates the continuous spraying system and adjusts the frequency and intensity of continuous spraying to restore to the ideal humidity. On the contrary, when the ambient humidity is too high, the system will reduce continuous spraying to avoid over-humidification, reduce the need for manual intervention, reduce operational complexity and potential human errors, and improve the overall hatching efficiency and success rate;

[0058] The nozzles are evenly distributed on the top of each layer of hatching racks, and the water droplets sprayed are small and uniform. Such water droplets can evaporate quickly and evenly increase the air humidity without leaving large water droplets on the equipment or horseshoe crab eggs, avoiding potential damage or pathogen growth caused by direct contact with water droplets, thereby reducing the risk of pathogen growth and improving the uniformity and success rate of hatching.

[0059] Please see attached Figure 1 In a preferred embodiment of the present invention, S4 comprises the following steps:

[0060] The indoor temperature control system includes an air conditioning device that automatically adjusts the temperature of the incubation environment in real time. By using an electronic temperature control device, precise temperature management can be achieved to ensure that the incubation environment is always kept within the ideal temperature range. This precise control helps to optimize the incubation process and reduce the impact of temperature fluctuations on the hatching of horseshoe crab eggs, thereby improving the efficiency and success rate of hatching. At the same time, automated temperature regulation reduces the need for manual monitoring and intervention, improving the convenience of operation and the reliability of the system.

[0061] The temperature control system can monitor the temperature of each layer through sensors and automatically adjust it to the set temperature range. This setting of individually monitoring the temperature of each layer can capture the specific temperature conditions of each layer, allowing the system to perform precise temperature control at each layer. When the temperature of a certain layer deviates from the set range, the control system can independently adjust the heating or cooling equipment of that layer without affecting the temperature of other layers, thereby improving the accuracy and flexibility of temperature management. At the same time, because only the layers that need to be adjusted are adjusted, unnecessary energy consumption is reduced.

[0062] Please see attached Figure 1 In a preferred embodiment of the present invention, S3 further comprises:

[0063] Continuous or intermittent spraying, the frequency range of intermittent spraying is set at 1 to 3 times per minute, and the duration of each spraying is adjusted between 10 seconds and 30 seconds according to the humidity conditions to ensure that the humidity of the hatching environment is always maintained between 60% and 80%. If the ambient humidity is monitored to be lower than 60%, the frequency of continuous spraying is increased or the duration of each continuous spraying is extended; conversely, if the humidity exceeds 80%, the frequency of continuous spraying is reduced or the continuous spraying time is shortened to gradually adjust to the target humidity range, which not only ensures the stability of the humidity of the hatching environment, but also avoids the development problems of horseshoe crab eggs caused by too low or too high humidity.

[0064] Please see attached Figure 1 In a preferred embodiment of the present invention, S5 includes:

[0065] The monitoring steps include visual inspection of the color and morphological changes of horseshoe crab eggs, using a stereo microscope to check the embryonic development, and combining sensors to monitor temperature and humidity parameters. Electron microscopes can capture tiny structural changes, which is crucial for early identification of developmental abnormalities. The combined use of visual inspection, electron microscopes and real-time sensor monitoring can comprehensively evaluate the incubation environment and the health status of horseshoe crab eggs, thereby achieving early diagnosis and intervention of problems and reducing hatching failures caused by environmental instability or abnormal embryonic development.

[0066] When hatching abnormalities are detected, the continuous spray intensity, frequency and indoor temperature are adjusted in time to ensure that the horseshoe crab eggs are hatched under optimal conditions. If the sensor detects that the humidity or temperature exceeds the set safety range, the system will adjust the intensity and frequency of the continuous spray system, or adjust the settings of the indoor temperature control equipment to quickly restore to the ideal hatching environment, thereby ensuring that the horseshoe crab eggs always develop under the best hatching conditions, greatly increasing the possibility of successful hatching. In addition, this automatic intervention reduces the need for manual adjustments, optimizes resource utilization, and improves the efficiency and reliability of the hatching process.

[0067] Please see attached Figure 1 In a preferred embodiment of the present invention, the method further comprises:

[0068] After hatching is completed, the net tray, hatching basin and hatching rack should be cleaned and disinfected. Cleaning and disinfection can remove the organic matter and microorganisms accumulated during the hatching process, thus avoiding threats to the next batch of horseshoe crab eggs to be hatched;

[0069] The cleaning step uses warm water to clean the incubation basin and mesh plate to remove surface residues. Warm water can effectively dissolve most organic matter and sediments and soften the residues fixed on the surface of the equipment. This step is mainly to remove dirt and organic residues that can be removed by simple rinsing. This is a necessary step before the disinfection process to ensure that the disinfectant can act more effectively on the surface of the equipment and reduce the microbial risk during the next incubation process.

[0070] The disinfection step uses high-temperature steam disinfection, disinfectants or ultraviolet sterilization devices for thorough disinfection to ensure that no pathogens or harmful substances remain during the next batch of incubation. The use of high-temperature steam disinfection or ultraviolet sterilization can ensure that all incubation tools are sterile, greatly reducing the risk of microbial contamination. These methods are not only efficient, but also environmentally friendly, avoiding the residue problems that may be caused by the use of chemical disinfectants;

[0071] After cleaning and disinfection, the equipment is dried and reassembled in preparation for the next batch of incubation operations. By thoroughly drying and reassembling the incubation equipment, it can be ensured that the equipment is kept in optimal condition when not in use, avoiding microbial growth or material damage caused by moisture. This process ensures the long-term availability of the equipment and the continuity of the incubation process, providing a guarantee for continued efficient incubation.

[0072] Please see attached Figure 1 In a preferred embodiment of the present invention, S4 further comprises:

[0073] Each layer of the hatching rack is equipped with an independent temperature and humidity control unit to ensure that the hatching conditions of each layer can be adjusted separately to meet the needs of different batches of horseshoe crab eggs or different developmental stages within the same batch. This design allows adjustment according to the specific needs of the internal conditions of each layer. For example, if the temperature of the bottom layer is too high due to its proximity to the heating equipment, the temperature of this layer can be lowered separately without affecting other layers. Similarly, if the humidity of the top layer is insufficient, the humidification frequency of only this layer can be increased, improving the flexibility and accuracy of environmental control, ensuring that each layer can provide the most suitable growth environment for horseshoe crab eggs, thereby improving the overall hatching success rate;

[0074] Each humidity control unit is connected through a central control system, which supports batch adjustment and layered independent control. This method allows operators to perform batch adjustment or independent control of specific layers as needed, so as to handle emergencies or perform specific experimental operations. Through the integrated management of the central control system, in addition, centralized control reduces the need for repeated settings, reduces energy consumption and operational complexity, and can effectively simplify the monitoring and adjustment of the incubation process and improve operational efficiency.

[0075] Please see attached Figure 1In a preferred embodiment of the present invention, the continuous spray system is linked to the central control system through a temperature sensor. When the temperature of the incubation environment is detected to exceed a preset upper limit, the system automatically starts the temperature adjustment mode. By integrating the temperature sensor with the central control system, the incubation equipment can monitor the temperature of each layer of the incubation environment in real time. When the sensor detects that the temperature exceeds the preset upper limit, the central control system automatically activates the temperature adjustment mode;

[0076] The continuous spraying system is linked to the central air-conditioning system through a temperature sensor. When it is detected that the incubation environment temperature exceeds a preset upper limit, the system automatically starts the temperature adjustment mode. When the ambient temperature returns to a predetermined range, the system gradually restores the normal frequency and duration of continuous spraying, and adjusts the continuous spraying interval according to the actual humidity to avoid excessive cooling or over-humidification. The continuous spraying interval is automatically adjusted to ensure that the incubation environment will not cause other problems due to over-adjustment after reaching the ideal temperature and humidity. This intelligent environmental management strategy optimizes resource utilization, avoids energy waste, and ensures the continuous stability of the incubation process, thereby improving the overall success rate and efficiency of incubation.

[0077] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for hatching horseshoe crab eggs in an air bath, characterized in that: The following steps are involved: S1. Construct a multi-layer hatching rack with each layer spaced at least 20 cm apart. Each layer is provided with a hatching basin, which is slightly tilted and has an opening at the bottom of the lower end. A drainage pipe is connected to the bottom for drainage. A water spray pipe is installed above the hatching basin. S2. Place a mesh plate in the incubation basin, 5 to 10 cm away from the bottom of the incubation basin, place a water-absorbing non-woven fabric on the mesh plate, and evenly distribute the horseshoe crab eggs on the moist non-woven fabric; S3. Use the spray system to spray each layer of the hatching rack continuously or intermittently to maintain appropriate humidity; S4. A heating air conditioning control system is installed indoors to adjust the indoor temperature in intervals of 0.5°C; S5. Spray continuously or intermittently and monitor temperature and humidity until hatching is completed.

2. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The S1 comprises the following steps: The hatching rack is made of corrosion-resistant materials, preferably stainless steel or anti-corrosion treated wood, and is placed indoors; The bottom of the hatching basin is slightly tilted to one side, a drainage outlet is arranged at the bottom of the basin to facilitate the discharge of excess water, and a water spray pipe is arranged above the hatching basin so that the water spray can cover the hatching basin.

3. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The S2 comprises the following steps: The net plate is composed of a frame and a sieve mesh. The sieve mesh is a nylon mesh with a mesh size of about 20, which can effectively support horseshoe crab eggs and non-woven fabrics. The non-woven fabric is a highly absorbent material and has the strength to maintain stability when wet, thus preventing the displacement of horseshoe crab eggs.

4. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The S3 comprises the following steps: The continuous spray system uses an automatic control device to adjust the intensity and frequency of continuous spraying according to the actual humidity; The nozzles are evenly distributed on the top of each layer of hatching racks, and the water droplets sprayed are small and uniform.

5. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The S4 comprises the following steps: The indoor temperature control system includes an air conditioning device that automatically adjusts the temperature of the incubation environment in real time.

6. The air bath hatching method for horseshoe crab eggs according to claim 4, characterized in that: The S3 further comprises: Continuous or intermittent spraying, the frequency range of intermittent spraying is set to 1 to 3 times per minute, and the duration of each spraying is adjusted between 10 seconds and 30 seconds according to the humidity conditions to ensure that the humidity of the hatching environment is always maintained between 60% and 80%.

7. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The S5 includes: The monitoring steps include visual inspection of horseshoe crab eggs for color and morphological changes, examination of embryonic development using a stereo microscope, and combined with sensors to monitor temperature and humidity parameters; When hatching abnormalities are detected, timely adjust the continuous spray intensity, frequency and indoor temperature to ensure that horseshoe crab eggs are hatched under optimal conditions.

8. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The method further comprises: After hatching is completed, clean and disinfect the net tray, hatching basin and hatching rack; Cleaning steps: Use warm water to clean the incubation basin and mesh plate to remove surface residues; The disinfection step uses high-temperature steam disinfection, disinfectants or ultraviolet disinfection devices to thoroughly disinfect and ensure that no pathogens or harmful substances remain during the next batch of incubation; Once cleaning and disinfection is complete, the equipment is dried and reassembled, ready for the next batch of hatching operations.

9. The air bath hatching method for horseshoe crab eggs according to claim 5, characterized in that: The S4 further comprises: Each layer of the hatching rack is equipped with an independent humidity control unit to ensure that the hatching conditions of each layer can be adjusted individually to meet the needs of different batches of horseshoe crab eggs or different developmental stages within the same batch; Each humidity control unit is connected through a central control system, supporting batch adjustment and layered independent control.

10. The air bath hatching method for horseshoe crab eggs according to claim 1, characterized in that: The continuous spray system is linked to the central air conditioning system through a temperature sensor. When the incubation environment temperature is detected to exceed a preset upper limit, the system automatically starts a temperature adjustment mode.

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