Pet halitosis-removing freeze-dried powder containing compound probiotics and preparation method of pet halitosis-removing freeze-dried powder

Abstract

The invention discloses a pet halitosis-removing freeze-dried powder containing compound probiotics and a preparation method thereof, and belongs to the field of pet oral cavity health care. The pet halitosis-removing freeze-dried powder containing the compound probiotics is prepared from the following components in percentage by mass: 95.5 percent to 99.5 percent of chicken breast, 0.01 percent to 0.1 percent of the compound probiotics, 0.1 percent to 0.8 percent of cellulose, 0.01 percent to 0.1 percent of an antioxidant and 0.05 percent to 0.1 percent of an additive. According to the freeze-dried pet feed, direct feeding can be achieved, tooth brushing operation is not needed, operation is convenient and easy, freeze-dried components are natural, feeding operation is easy, and the risk that a pet owner is scratched is avoided.

Description

Technical Field

[0001] The invention belongs to the field of pet oral health care, and particularly relates to a pet breath-eliminating freeze-dried product containing composite probiotics and a preparation method thereof. Background Art

[0002] The oral health of pets (dogs and cats) has always been an aspect that pet owners easily overlook. Nearly half of pets have bad breath to varying degrees. The bad breath of pet dogs and cats can be roughly divided into pathological bad breath and physiological bad breath. Physiological bad breath is mostly short-lived and has less impact. Among pathological bad breath, it is specifically divided into: food residues will ferment in the mouth, forming dental plaque and producing bad breath; the disorder of intestinal flora causes the massive reproduction of Helicobacter pylori, producing bad breath. Therefore, to control the bad breath of pet dogs and cats, it is necessary to prevent or eliminate the bad breath of pets from two aspects: dental plaque and intestinal flora.

[0003] As pet owners become younger, they pay more attention to their pets' oral health. However, dogs and cats have no habit of brushing their teeth and are reluctant to do so. Brushing their teeth can easily cause redness and swelling of their gums, gingivitis, and other symptoms. Products related to pet oral care have been introduced to the market, such as tooth cleaning sticks, mouthwashes, and sprays, to address pet oral health in various situations. However, these current products are slow to take effect and only treat the symptoms but not the root cause.

[0004] Studies have shown that probiotics can adhere to the surface of dog and cat teeth, thereby regulating the composition of the microbiome and promoting oral health. When feeding granular freeze-dried food containing probiotics, the dirt and plaque on the teeth of dogs and cats are removed by friction. In addition, probiotics can change the diversity and abundance of intestinal microbial flora, and their metabolites can reduce the local pH of the intestine, providing a suitable acidic environment for the growth and colonization of beneficial intestinal flora and inhibiting the reproduction and growth of pathogenic bacteria. Cellulose encapsulates probiotics to provide them with a suitable growth environment. Since fatty acid oxidation is a common problem in freeze-dried meat products, the selection of good natural antioxidants is particularly important. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a pet breath-eliminating freeze-dried product containing composite probiotics and a preparation method thereof, which can be fed directly without brushing teeth and is convenient and simple to operate. The freeze-dried product has natural ingredients and is easy to feed, thus avoiding the risk of pet owners being scratched.

[0006] The technical scheme adopted by the present invention is: a pet deodorant freeze-dried product containing compound probiotics, which comprises the following components by mass percentage: 95.5%-99.5% chicken breast, 0.01%-0.1% compound probiotics, 0.1%-0.8% cellulose, 0.01%-0.1% antioxidant and 0.05%-0.1% additive.

[0007] In one of the embodiments, the freeze-dried pet deodorant containing compound probiotics comprises the following components by mass percentage: 99.3% chicken breast, 0.05% compound probiotics, 0.5% cellulose, 0.05% antioxidant and 0.1% additive.

[0008] In one embodiment, the composite probiotics are at least two of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Bifidobacterium breve, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus rhamnosus or Bifidobacterium longum subsp. longum.

[0009] In one embodiment, the cellulose is at least one of dextran, chitosan, guar gum, sodium alginate, barley, flaxseed, psyllium husk, oats or agar.

[0010] In one embodiment, the cellulose is flaxseed, psyllium husk or oat.

[0011] In one embodiment, the antioxidant is at least two of BHT, BHA, tocopherol, tea polyphenols, mint or thymol.

[0012] In one embodiment, the antioxidant is tea polyphenols.

[0013] In one embodiment, the additive is at least two of vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin, calcium pantothenate, folic acid or niacinamide.

[0014] In one embodiment, the additive is vitamin B2 or vitamin B6.

[0015] The present invention also discloses a preparation method of a pet deodorant freeze-dried product containing a composite probiotic, which is used to prepare the pet deodorant freeze-dried product containing a composite probiotic, and comprises the following steps:

[0016] Step 1, weigh each component according to mass percentage for later use;

[0017] Step 2: Cook the chicken breast in boiling water, cool naturally to room temperature, grind into uniform powder in a meat grinder, and set aside;

[0018] Step 3: Place the ground chicken breast in a constant temperature water bath, set the temperature of the constant temperature water bath to 37°C, add 50ml UP water to the beaker, and keep the constant temperature water bath for 2h;

[0019] Step 4: Add cellulose, compound probiotics, antioxidants and additives into an empty beaker, stir evenly to completely dissolve the additives, and obtain a mixture;

[0020] Step 5: Place the obtained mixture in a constant temperature water bath for later use;

[0021] Step 6: Add the mixture to the minced chicken in small amounts several times and stir evenly to obtain a mixed meat paste;

[0022] Step 7: Pour the mixed meat paste into a mold, pre-freeze it at -60°C for 8 hours, and then freeze-dry it in a freeze dryer for 24 hours. After demolding, the pet deodorizing freeze-dried product containing compound probiotics is obtained.

[0023] The beneficial effects of the present invention are as follows: the freeze-dried pet deodorizing product containing the composite probiotics can effectively reduce dental plaque during the chewing process of the pet; after swallowing, the composite probiotics regulate the intestinal flora in the intestine; the freeze-dried product can be directly fed without brushing teeth, and the operation is convenient and simple, avoiding the risk of scratching the pet owner; and the freeze-dried product has a clean ingredient list and a simple preparation method, and is suitable for industrial production. DETAILED DESCRIPTION

[0024] The present invention will be further described in detail below with reference to specific embodiments.

[0025] Embodiment 1:

[0026] The invention discloses a pet breath-eliminating freeze-dried product containing composite probiotics. The pet breath-eliminating freeze-dried product containing composite probiotics comprises the following components by mass percentage: 99.3% chicken breast meat, 0.05% composite probiotics, 0.5% cellulose, 0.05% antioxidant and 0.1% additive.

[0027] In this embodiment, the composite probiotics are at least two of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Bifidobacterium breve, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus rhamnosus or Bifidobacterium longum subsp. longum.

[0028] In this embodiment, the cellulose is at least one of dextran, chitosan, guar gum, sodium alginate, barley, flaxseed, psyllium husk, oats or agar.

[0029] In this embodiment, the cellulose is flaxseed, psyllium husk or oat.

[0030] In this embodiment, the antioxidants are at least two of BHT, BHA, tocopherol, tea polyphenols, mint or thymol.

[0031] In this embodiment, the antioxidant is tea polyphenols.

[0032] In this embodiment, the additive is at least two of vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin, calcium pantothenate, folic acid or niacinamide.

[0033] In this embodiment, the additive is vitamin B2 or vitamin B6.

[0034] Embodiment 2:

[0035] The invention discloses a pet deodorant freeze-dried product containing composite probiotics. The pet deodorant freeze-dried product containing composite probiotics comprises the following components by mass percentage: 99.2% chicken breast, 0.05% composite probiotics, 0.6% cellulose, 0.05% antioxidant and 0.1% additive.

[0036] In this embodiment, the composite probiotics are at least two of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Bifidobacterium breve, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus rhamnosus or Bifidobacterium longum subsp. longum.

[0037] In this embodiment, the cellulose is at least one of dextran, chitosan, guar gum, sodium alginate, barley, flaxseed, psyllium husk, oats or agar.

[0038] In this embodiment, the cellulose is flaxseed, psyllium husk or oat.

[0039] In this embodiment, the antioxidants are at least two of BHT, BHA, tocopherol, tea polyphenols, mint or thymol.

[0040] In this embodiment, the antioxidant is tea polyphenols.

[0041] In this embodiment, the additive is at least two of vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin, calcium pantothenate, folic acid or niacinamide.

[0042] In this embodiment, the additive is vitamin B2 or vitamin B6.

[0043] Embodiment 3:

[0044] The invention discloses a pet deodorant freeze-dried product containing composite probiotics. The pet deodorant freeze-dried product containing composite probiotics comprises the following components by mass percentage: 99.2% chicken breast, 0.05% composite probiotics, 0.55% cellulose, 0.1% antioxidant and 0.1% additive.

[0045] In this embodiment, the composite probiotics are at least two of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Bifidobacterium breve, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus rhamnosus or Bifidobacterium longum subsp. longum.

[0046] In this embodiment, the cellulose is at least one of dextran, chitosan, guar gum, sodium alginate, barley, flaxseed, psyllium husk, oats or agar.

[0047] In this embodiment, the cellulose is flaxseed, psyllium husk or oat.

[0048] In this embodiment, the antioxidants are at least two of BHT, BHA, tocopherol, tea polyphenols, mint or thymol.

[0049] In this embodiment, the antioxidant is tea polyphenols.

[0050] In this embodiment, the additive is at least two of vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin, calcium pantothenate, folic acid or niacinamide.

[0051] In this embodiment, the additive is vitamin B2 or vitamin B6.

[0052] In the above three embodiments, since flaxseed, psyllium husk and oats are all rich in natural dietary fiber ingredients, cellulose is preferably flaxseed, psyllium husk or oats. Tea polyphenols are all natural antioxidant foods extracted from tea leaves. Because they have strong antioxidant capacity, no toxic side effects, no odor and other characteristics, antioxidants are preferably tea polyphenols. Since vitamin B2 can prevent and eliminate inflammation in the oral cavity and promote the normal growth of skin and hair; vitamin B6 has physiological functions such as anti-inflammatory, anti-oxidation, neuromodulation and anti-tumor, which is essential to maintain the health of the nervous system, skin health and immune function; therefore, the additive is preferably vitamin B2 or vitamin B6.

[0053] The preparation methods of the freeze-dried pet deodorant containing composite probiotics described in the above three embodiments are consistent, and include the following steps:

[0054] Step 1, weigh each component according to mass percentage for later use;

[0055] Step 2: Cook the chicken breast in boiling water, cool naturally to room temperature, grind into uniform powder in a meat grinder, and set aside;

[0056] Step 3: Place the ground chicken breast in a constant temperature water bath, set the temperature of the constant temperature water bath to 37°C, add 50ml UP water to the beaker, and keep the constant temperature water bath for 2h;

[0057] Step 4: Add cellulose, compound probiotics, antioxidants and additives into an empty beaker, stir evenly to completely dissolve the additives, and obtain a mixture;

[0058] Step 5: Place the obtained mixture in a constant temperature water bath for later use;

[0059] Step 6: Add the mixture to the minced chicken in small amounts several times and stir evenly to obtain a mixed meat paste;

[0060] Step 7: Pour the mixed meat paste into a mold, pre-freeze it at -60°C for 8 hours, and then freeze-dry it in a freeze dryer for 24 hours. After demolding, the pet deodorizing freeze-dried product containing compound probiotics is obtained.

[0061] In the above method, in step 2, the chicken breast is ground in a meat grinder until 95% of the chicken breast can pass through a No. 5 sieve. In step 5, the obtained mixture is placed in a 37° C. constant temperature water bath.

[0062] In the above-mentioned embodiment, the mechanism of action of probiotics includes that it can adhere to the surface of dog and cat teeth, achieving the effect of regulating microbial composition and promoting oral health. When feeding granular freeze-dried food containing probiotics, dirt and plaque on dog and cat teeth are removed by friction. In addition, probiotics can change the diversity and abundance of intestinal microbial flora, and its metabolites reduce the local pH of the intestine, provide a suitable acidic environment for the growth and colonization of beneficial intestinal flora and inhibit the reproduction and growth of pathogenic bacteria (Helicobacter pylori, etc.), greatly reducing the probability of halitosis. Cellulose encapsulates probiotics to provide them with a suitable growth environment. Since fatty acid oxidation is a common problem in meat freeze-dried products, the selection of good natural antioxidants is particularly important.

[0063] In order to test the effects of the freeze-dried pet deodorant containing composite probiotics prepared in the above three embodiments, an animal feeding test experiment was conducted, and the test results are as follows:

[0064] (1) Experimental method: Five freeze-dried pet deodorant containing composite probiotics prepared by the components of Example 1, Example 2 and Example 3 were respectively mixed into the animal diet and numbered as samples No. 1 / 2 / 3, respectively.

[0065] (2) Experimental animals: 32 pet dogs and 32 pet cats with obvious halitosis were recruited and randomly divided into 8 groups: Groups 1 / 2 / 3 / 4 were all pet dogs, of which Groups 1 / 2 / 3 were experimental groups and Group 4 was a control group; Groups 5 / 6 / 7 / 8 were all pet cats, of which Groups 5 / 6 / 7 were experimental groups and Group 8 was a control group. Experimental groups 1 / 5, 2 / 6, and 3 / 7 were fed diets containing experimental samples 1 / 2 / 3, respectively, and the control group was fed blank diets. Feeding was continued for 7 days, and the improvement of halitosis was observed after 7 days.

[0066] (3) Rating criteria: A 15-member oral odor evaluation team was formed, with 5 evaluators aged 20-30 years old, 5 evaluators aged 30-40 years old, and 5 evaluators aged 40-60 years old.

[0067] a: Open the mouths of dogs and cats, and let 15 people in the evaluation team smell the odor, and more than 90% of them judge that there is no odor;

[0068] b: The mouths of dogs and cats were opened, and 15 people from the evaluation team smelled the odor, and more than 90% of them judged that there was a slight odor;

[0069] c: The mouths of dogs and cats were opened, and 15 people from the evaluation team smelled the odor, and more than 90% of them judged that there was an odor;

[0070] d: The mouths of dogs and cats were opened, and 15 people from the evaluation team smelled the odor, and more than 90% of them judged that the odor was strong;

[0071] e: The mouths of dogs and cats were opened and 15 people from the evaluation team smelled the odor, and more than 90% of them judged that there was an unpleasant odor.

[0072] (4) Experimental results

[0073] The experimental results are shown in Table 1.

[0074] Table 1 Oral odor evaluation results

[0075]

[0076]

[0077] As can be seen from Table 1, after 7 days of feeding, there were 6, 6, and 5 dogs without odor in Experimental Groups 1, 2, and 3, respectively, while there were 7 dogs with unpleasant odor in Control Group 4, and 1 dog with a relatively strong odor, indicating that Experimental Groups 1, 2, and 3 had significant effects on improving bad odor in the mouths of pet dogs, among which Sample 1 was the best, with significant differences compared with the control group; there were 6, 5, and 5 cats without odor in Experimental Groups 5, 6, and 7, respectively, while there were 7 cats with unpleasant odor in Control Group 8, and 1 cat with a relatively strong odor, indicating that Experimental Groups 5, 6, and 7 had significant effects on improving bad odor in the mouths of pet dogs, among which Sample 1 was the best, with significant differences compared with the control group.

[0078] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A freeze-dried product for removing bad breath of pets containing composite probiotics, characterized in that: The pet deodorant freeze-dried product containing compound probiotics comprises the following components by mass percentage: 95.5%-99.5% chicken breast, 0.01%-0.1% compound probiotics, 0.1%-0.8% cellulose, 0.01%-0.1% antioxidant and 0.05%-0.1% additive.

2. The freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 1, characterized in that: The pet deodorant freeze-dried product containing compound probiotics comprises the following components by mass percentage: 99.3% chicken breast, 0.05% compound probiotics, 0.5% cellulose, 0.05% antioxidant and 0.1% additive.

3. A freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 1 or 2, characterized in that: The composite probiotics are at least two of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Bifidobacterium breve, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus rhamnosus or Bifidobacterium longum subsp. longum.

4. A freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 1 or 2, characterized in that: The cellulose is at least one of dextran, chitosan, guar gum, sodium alginate, barley, flaxseed, psyllium husk, oat or agar.

5. The method for preparing a freeze-dried pet deodorant containing composite probiotics according to claim 4, characterized in that: The cellulose is flaxseed, psyllium husk or oat.

6. A freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 1 or 2, characterized in that: The antioxidant is at least two of BHT, BHA, tocopherol, tea polyphenols, mint or thymol.

7. The freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 6, characterized in that: The antioxidant is tea polyphenols.

8. A freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 1 or 2, characterized in that: The additives are at least two of vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin, calcium pantothenate, folic acid or niacinamide.

9. The freeze-dried product for removing bad breath of pets containing composite probiotics according to claim 8, characterized in that: The additive is vitamin B2 or vitamin B6.

10. A method for preparing a freeze-dried pet deodorant containing a composite probiotic, which is used to prepare the freeze-dried pet deodorant containing a composite probiotic according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1, weigh each component according to mass percentage for later use; Step 2: Cook the chicken breast in boiling water, cool naturally to room temperature, grind into uniform powder in a meat grinder, and set aside; Step 3: Place the ground chicken breast in a constant temperature water bath, set the temperature of the constant temperature water bath to 37°C, add 50ml UP water to the beaker, and keep the constant temperature water bath for 2h; Step 4: Add cellulose, compound probiotics, antioxidants and additives into an empty beaker, stir evenly to completely dissolve the additives, and obtain a mixture; Step 5: Place the obtained mixture in a constant temperature water bath for later use; Step 6: Add the mixture to the minced chicken in small amounts several times and stir evenly to obtain a mixed meat paste; Step 7: Pour the mixed meat paste into a mold, pre-freeze it at -60°C for 8 hours, and then freeze-dry it in a freeze dryer for 24 hours. After demolding, the pet deodorizing freeze-dried product containing compound probiotics is obtained.

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