Preparation for enhancing maternal property, preparation method of preparation and application of preparation in preventing animals from eating cubs
Through a preparation containing vegetable oil, ginger extract, plant polysaccharides and specific essential oils, the problem of eating pups during animal reproduction is solved. Through olfactory training and hormone regulation, the rate of eating pups is significantly reduced and the instinct of motherhood is enhanced.
Patent Information
- Application Number
- CN202411538650.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-31
- Publication Date
- 2025-02-14
AI Technical Summary
The prior art is difficult to effectively avoid the problem of animals eating pups during reproduction, especially because female mice are prone to irritation during production and breastfeeding, resulting in accidental injury or refusal to breastfeed.
Provide a preparation that enhances maternity, including vegetable oil, ginger extract, plant polysaccharides, sodium chloride and water, and forms vegetable oil through specific proportions of chamomile essential oil and orange peel oil to regulate the animal's sense of smell and hormone levels and enhance the maternity's instinct. .
Through olfactory training and hormone regulation, the preparation improves the ability of female mice to distinguish the odor of the puppies, reduces the rate of eating puppies, and relieves anxiety and depression during the reproduction process, thereby enhancing maternal behavior.
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Abstract
Description
Technical Field
[0001] The present application relates to the technical field of drugs for enhancing animal reproduction, and in particular to a preparation for enhancing maternal ability, a preparation method thereof, and an application thereof in preventing animals from cannibalizing their offspring. Background Art
[0002] The sense of smell is ubiquitous in the animal kingdom and is essential to the lives of many creatures. The sense of smell is not only the sense of distinguishing odors, but can also play a role in danger alarm, affecting appetite, regulating emotions, etc. under certain circumstances. Among them, mice have a sensitive sense of smell and are afraid of irritating odors. They are more sensitive to odors. The breeding environment of pregnant and lactating mice should be comfortable and large, and it is necessary to minimize and avoid human influences on the birth and lactation of female mice, including the stimulation of light, noise and odor.
[0003] The olfactory pathways of animals mainly include the main olfactory system and the vomeronasal organ. The main olfactory system is the main receiver of volatile small molecule chemical signals; the vomeronasal organ is the receptor of non-volatile substances and is a special organ that receives intraspecific pheromones (related to reproduction, attack, and defense).
[0004] The process of giving birth is very physically demanding for pregnant mice, and the mother mouse usually replenishes her energy by eating the placenta and fetal membranes. The pups that have just come out of the birth canal are wrapped in the fetal membranes, and the mother mouse needs to tear the fetal membranes to breathe the first breath of fresh air. The mother mouse licks the pups while swallowing the placenta and fetal membranes, and may accidentally injure the pups in the process, and the pups may be eaten as supplements; in addition, if the mother mouse smells a special smell on the pups, the pups are likely to be eaten.
[0005] The current method available on the market to reduce the cannibalism of animals is generally to use alcohol spray, but alcohol is extremely volatile, highly irritating, and has poor durability, and thus cannot achieve the desired effect. Summary of the invention
[0006] In order to solve the above technical problems, the present application provides a maternal enhancement preparation and a preparation method thereof and an application thereof in preventing animals from cannibalizing their cubs.
[0007] In the first aspect, the present application provides a preparation for enhancing maternal ability, specifically comprising the following components in parts by weight: 10-20 parts of vegetable oil, 0.5-1.5 parts of ginger extract, 0.2-0.8 parts of plant polysaccharides, 0.8-2.0 parts of sodium chloride, and 100 parts of water; the vegetable oil is a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.5-1.5:0.5-1.5.
[0008] The preparation provided in the present application can effectively train the sense of smell of animals, allow the animals to adapt to the existing smells, identify the smell of the pups and the smell of the mother mouse's original living environment, temporarily increase the sense of smell to perceive and distinguish the smell of the pups, and reduce the mice's cannibalism; at the same time, the preparation can increase the levels of estradiol, prolactin, and progesterone in the mother mouse, relieve the anxiety and depression of the experimental mother mice during the breeding process, enhance their maternal instinct, and further reduce the mice's cannibalism rate.
[0009] Estradiol is a natural estrogen produced by ovarian follicles, corpus luteum and placenta during pregnancy. Studies have found that it can regulate nerve function, enhance the expression of its receptors and nerve growth factor, regulate nerve function, and thus play an anti-depressant role; prolactin has certain anti-anxiety and analgesic effects, and progesterone helps the synthesis of serotonin, which promotes the body to produce a happy mood. During pregnancy, prolactin and progesterone are at a high level in experimental animals, which can reduce tension and anxiety during pregnancy. After giving birth, the hormone levels of experimental animals undergo tremendous changes. If the hormone levels drop too fast, it is easy to cause corresponding depression and behavioral changes, causing anxiety and depression in the mother mouse. In this case, the amount of milk secretion decreases, which will cause the young mice to compete for milk and bite the mother mouse's nipples. The mother mouse refuses to breastfeed due to the pain of breastfeeding, and even bites the young mice.
[0010] The liquid formulation of the preparation provided in this application mainly comprises chamomile essential oil, sweet orange peel oil, ginger extract, and plant polysaccharides, and does not contain alcohol or essence. Among them, chamomile essential oil has the effect of helping sleep, and has an excellent soothing effect. It can relieve anxiety, tension, anger and fear, and make animals relax and patient, and feel peaceful; it can relieve worries and calm the mind, which is very helpful for insomnia; it also has the function of analgesia, which can relieve muscle pain, especially pain caused by nervous tension, and is also very helpful for lower back pain; the same effect can also calm headaches, neuralgia, toothaches and earaches. Sweet orange peel oil has sedative and soothing properties, which is helpful to relieve anxiety and stress. Through aromatherapy, sweet orange peel oil can promote relaxation of the nervous system, improve mood, and relieve anxiety and tension. These fragrance molecules pass through the nasal cavity, stimulate the olfactory nerves, and are transmitted to the brain, thereby producing a soothing and sleep-aiding effect on animals.
[0011] The applicant of the present application discovered during the experimental process that vegetable oil is composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.5-1.5:0.5-1.5, and scientific experiments have verified that a preparation is prepared by mixing vegetable oil in specific weight portions with specific amounts of ginger extract, plant polysaccharides, sodium chloride, and water. The synergistic effects of the raw material components can increase the hormone level in the animal's body or adjust its nervous system activity, enhance its maternal instinct, have a complex regulatory effect on maternal behavior, regulate maternal aggressive behavior, arouse its innate desire to protect, make it more caring and protective of its cubs, and reduce the occurrence of cub-eating behavior.
[0012] The ingredients of this preparation are non-toxic and harmless. After testing, most of them are used in the ingredients of various skin care products and perfumes. They are harmless to animals, and have better fragrance retention and persistence. In this way, the odor molecules can maintain the odor in the mouse cage for a longer time, allowing the mother mouse to adapt to the odor of the entire environment in advance. When the pups are born, the corresponding liquid is also sprayed on the pups so that the mother mouse can identify the odor in time and prevent animals from eating the pups.
[0013] Preferably, the maternal enhancement preparation specifically comprises the following components in parts by weight: 13-18 parts of vegetable oil, 0.8-1.2 parts of ginger extract, 0.4-0.6 parts of plant polysaccharides, 1.2-1.7 parts of sodium chloride, and 100 parts of water.
[0014] Preferably, the maternal enhancement preparation specifically comprises the following components in parts by weight: 15 parts of vegetable oil, 1 part of ginger extract, 0.5 parts of plant polysaccharides, 1.5 parts of sodium chloride, and 100 parts of water.
[0015] Preferably, the vegetable oil is a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.2-1.5:0.5-0.8.
[0016] Preferably, the plant polysaccharide is selected from one or more of wolfberry polysaccharide, ganoderma lucidum polysaccharide, tuckahoe polysaccharide and cordyceps polysaccharide.
[0017] Preferably, the plant polysaccharide is composed of a mixture of wolfberry polysaccharide and tuckahoe polysaccharide in a weight ratio of 11:1-5.
[0018] Preferably, the plant polysaccharide is composed of a mixture of wolfberry polysaccharide and tuckahoe polysaccharide in a weight ratio of 11:2-4.
[0019] In a second aspect, the present application provides a method for preparing the above-mentioned maternal enhancement preparation, which specifically comprises the following steps: respectively weighing the corresponding weights of the raw material components, mixing sodium chloride and water to prepare a sodium chloride aqueous solution, and then respectively adding vegetable oil, ginger extract and plant polysaccharides to the sodium chloride aqueous solution to obtain.
[0020] In a third aspect, the present application provides the use of the above-mentioned maternal enhancement preparation in preventing animals from cannibalizing their young.
[0021] In a fourth aspect, the present application provides the above-mentioned preparation for olfactory training of experimental animals to prevent animals from eating their cubs, comprising the above-mentioned preparation for enhancing maternal instinct.
[0022] In summary, the technical solution of this application has the following effects: The application uses a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.5-1.5:0.5-1.5 to form a vegetable oil, and a preparation is prepared with a specific weight of vegetable oil and a specific amount of ginger extract, plant polysaccharide, sodium chloride, and water. The preparation can effectively train the sense of smell of animals, allow the animals to adapt to existing smells, identify the smell of pups and the smell of the mother mouse's original living environment, and temporarily increase the sense of smell to perceive and distinguish the smell of pups; at the same time, the preparation can increase the levels of estradiol E2, prolactin PRL, and progesterone PROG in the mother mouse, relieve the anxiety and depression of the experimental mother mice during the breeding process, enhance their maternal instinct, and thereby reduce the rate of mice eating their pups.
[0023] This application is verified through scientific experiments, and the types and dosage ratios of vegetable oils and plant polysaccharides are screened and optimized, thereby further improving the application performance of the preparation. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 These are photographs of mice in Example 2 on the 2nd, 4th, 8th and 10th days after birth (a - 2nd day, b - 4th day, c - 2nd day, d - 10th day).
[0025] Figure 2 These are photographs of mice in Comparative Example 1 on the 2nd, 4th, 8th and 10th days after birth (a - 2nd day, b - 4th day, c - 2nd day, d - 10th day). DETAILED DESCRIPTION
[0026] The present application is further described in detail below in conjunction with examples, comparative examples and performance testing experiments. These examples should not be construed as limiting the scope of protection claimed in the present application.
[0027] The sweet orange peel oil used in this application was purchased from the main store of Runshuo natural plant essential oil brand; ginger extract and red date extract were purchased from Xinyang Mufan Biotechnology Co., Ltd.; chamomile essential oil, gardenia essential oil, wolfberry polysaccharide (item number: B20460-500mg), Ganoderma lucidum polysaccharide (item number: S27814), Poria cocos polysaccharide (item number: S25272), and Cordyceps polysaccharide (item number: B24633) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Example
[0028] Examples 1-7 Examples 1-7 provide a maternal enhancement preparation The difference between the above embodiments is that the dosage of each component in the preparation is different, as shown in Table 1.
[0029] The preparation method of the preparation in the above embodiment is as follows: as shown in Table 1, the corresponding weights of the raw material components are weighed respectively, sodium chloride and water are mixed to prepare a sodium chloride aqueous solution, and then vegetable oil (composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.4:0.6), ginger extract and plant polysaccharide (composed of a mixture of wolfberry polysaccharide and Poria cocos polysaccharide in a weight ratio of 11:3) are added to the sodium chloride aqueous solution respectively to obtain Table 1 Amount of each component in the preparations of Examples 1-7 Embodiment 8-11 Examples 8-11 provide a maternal enhancement preparation The difference between the above embodiment and embodiment 2 is that the types of vegetable oils are different, as shown below.
[0030] In Example 8: the vegetable oil is composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.6:1.4.
[0031] In Example 9: the vegetable oil is composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.2:0.9.
[0032] In Example 10: the vegetable oil is composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.2:0.8.
[0033] In Example 11: the vegetable oil is composed of a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.5:0.5.
[0034] The other raw materials and their amounts and the preparation method of the preparation in the above examples are the same as those in Example 2.
[0035] Examples 12-17 Examples 12-17 each provide a maternal enhancement formulation.
[0036] The difference between the above embodiment and embodiment 2 is that the types of vegetable oils are different, as shown below.
[0037] In Example 12: the plant polysaccharide is pachymaran.
[0038] In Example 13: the plant polysaccharide is composed of a mixture of Ganoderma lucidum polysaccharide and Poria cocos polysaccharide in a weight ratio of 11:3.
[0039] In Example 14: the polysaccharide is composed of a mixture of wolfberry polysaccharide and cordyceps polysaccharide in a weight ratio of 11:3.
[0040] In Example 15: the plant polysaccharide is composed of a mixture of wolfberry polysaccharide and poria polysaccharide in a weight ratio of 3:11.
[0041] In Example 16: the plant polysaccharide is composed of a mixture of wolfberry polysaccharide and poria polysaccharide in a weight ratio of 11:1.
[0042] In Example 17: the plant polysaccharide is composed of a mixture of wolfberry polysaccharide and poria polysaccharide in a weight ratio of 11:5.
[0043] The other raw materials and their amounts and the preparation method of the preparation in the above examples are the same as those in Example 2.
[0044] Comparative Example Comparative Examples 1-3 Comparative Examples 1-3 each provide a preparation.
[0045] The difference between the comparative example and Example 2 is that the dosage of each component in the preparation is different, as shown in Table 2.
[0046] Table 2 The dosage of each component in the preparations of Comparative Examples 1-3 The other raw materials and their amounts and the preparation method of the preparation in the above comparative example are the same as those in Example 2.
[0047] Comparative Examples 4-6 Comparative Examples 4-6 each provide a preparation.
[0048] The differences between the above comparative example and Example 2 are specifically as follows.
[0049] In Comparative Example 4, the vegetable oil is composed of a mixture of gardenia essential oil and sweet orange peel oil in a weight ratio of 1.4:0.6.
[0050] In Comparative Example 5: the vegetable oil is chamomile essential oil.
[0051] In Comparative Example 6: an equal amount of red date extract was used to replace the ginger extract.
[0052] The other raw materials and their amounts and the preparation method of the preparation in the above comparative example are the same as those in Example 2.
[0053] Comparative Example 7 Comparative Example 7 provides a preparation using physiological saline as the preparation.
[0054] Performance testing Animal selection: C57BL / 6J pregnant mice, 14 days of gestation, 6 mice in each group.
[0055] Reagent selection: administration by spray.
[0056] Experimental groups: 6 mice in each group. The experimental group of mother mice in the middle and late stages of pregnancy, the mother mice were sprayed twice a day. On the second day of the newborn mice, all the pups were sprayed; the blank group of mother mice in the middle and late stages of pregnancy, the mother mice and the newborn mice were not treated every day.
[0057] (1) Observation results: Observe and record the number of mice born, and continuously record whether the mother mice eat their pups. Observation results: as shown in Table 3.
[0058] During the observation process, no mother rat cannibalism occurred in Examples 1-17; Figure 1 As shown, the photos of mice in Example 2 on the 2nd, 4th, 8th and 10th days after birth (a-2nd day, b-4th day, c-2nd day, d-10th day). In Comparative Examples 1-7, the phenomenon of mother mice eating their pups occurred; Figure 2 Shown are photographs of mice in Comparative Example 1 on the 2nd, 4th, 8th and 10th days after birth (a - 2nd day, b - 4th day, c - 2nd day, d - 10th day).
[0059] (2) Sample collection and testing: On the 4th day after the birth of the newborn mice, the mother mice were fasted for 12 h, anesthetized with 10% chloral hydrate, and 4.0 mL of blood was collected from the abdominal aorta. The blood sample was allowed to stand at room temperature for 1 h. After the blood sample coagulated, it was centrifuged at 3000 r / min for 10 min. The upper serum was collected and stored at -20°C. The double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of estradiol E2, prolactin PRL, and progesterone PROG in the mother mice using immunohistochemistry kits.
[0060] Test results: as shown in Table 3.
[0061] Table 3 Application performance test results of the preparations in Examples 1-17 and Comparative Examples 1-7 Combined with Table 3, by comparing the test results of Examples 1-17 with those of Comparative Examples 1-7, it can be seen that the present application uses a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.5-1.5:0.5-1.5 to form a vegetable oil, and a specific weight portion of vegetable oil and a specific amount of ginger extract, plant polysaccharide, sodium chloride, and water to prepare a preparation, which can effectively train the sense of smell of animals, allow the animals to adapt to the existing smell, identify the smell of the pups and the smell of the original living environment of the mother mouse, and temporarily increase the sense of smell to perceive and distinguish the smell of the pups; at the same time, the preparation can increase the levels of estradiol E2, prolactin PRL, and progesterone PROG in the mother mouse, relieve the anxiety and depression of the experimental mother mice during the breeding process, enhance their maternal instinct, and thereby reduce the rate of mice eating their pups.
[0062] In Comparative Example 1, sodium chloride aqueous solution alone was used as the preparation. In Comparative Example 4, the vegetable oil was a mixture of gardenia essential oil and sweet orange peel oil in a weight ratio of 1.4:0.6. In Comparative Example 5, the vegetable oil was chamomile essential oil. In Comparative Example 6, an equal amount of red date extract was used instead of ginger extract. The application performance of the preparations prepared in the above comparative examples was poor.
[0063] By comparing the test results of Examples 1-7 with those of Comparative Examples 2-3, it can be seen that the dosage of each component raw material in the preparation of the present application has a significant effect on the performance of the female mice. By controlling the dosage of each raw material component, the present application can effectively enable the prepared preparation to obtain excellent results.
[0064] By comparing the test results of Examples 2 and 8-11, the present application further improves the performance of the preparation for experimental animals by controlling the vegetable oil to be a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 1.2-1.5:0.5-0.8.
[0065] By comparing the test results of Examples 2 and 12-17, the present application further improves the performance of the preparation for experimental animals by mixing plant polysaccharides composed of wolfberry polysaccharides and poria polysaccharides in a weight ratio of 11:1-5.
[0066] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.
Claims
1. A preparation for enhancing maternity, characterized in that Specifically, it includes the following components in parts by weight: 10-20 parts of vegetable oil, 0.5-1.5 parts of ginger extract, 0.2-0.8 parts of plant polysaccharides, 0.8-2.0 parts of sodium chloride, and 100 parts of water; the vegetable oil is a mixture of chamomile essential oil and sweet orange peel oil in a weight ratio of 0.5-1.5:0.5-1.
5.
2. The maternity enhancing preparation according to claim 1, characterized in that Specifically, the invention comprises the following components in parts by weight: 13-18 parts of vegetable oil, 0.8-1.2 parts of ginger extract, 0.4-0.6 parts of plant polysaccharide, 1.2-1.7 parts of sodium chloride and 100 parts of water.
3. The maternity enhancing preparation according to claim 1, characterized in that Specifically, it includes the following components in parts by weight: 15 parts of vegetable oil, 1 part of ginger extract, 0.5 parts of plant polysaccharides, 1.5 parts of sodium chloride, and 100 parts of water.
4. The maternity enhancing preparation according to claim 1, characterized in that The vegetable oil is prepared by mixing chamomile essential oil and sweet orange peel oil in a weight ratio of 1.2-1.5:0.5-0.
8.
5. The maternity-enhancing preparation according to claim 1, characterized in that The plant polysaccharide is selected from one or more of wolfberry polysaccharide, ganoderma lucidum polysaccharide, tuckahoe polysaccharide and cordyceps polysaccharide.
6. The maternity-enhancing preparation according to claim 5, characterized in that The plant polysaccharide is composed of a mixture of wolfberry polysaccharide and tuckahoe polysaccharide in a weight ratio of 11:1-5.
7. The maternity enhancing preparation according to claim 6, characterized in that The plant polysaccharide is composed of a mixture of wolfberry polysaccharide and tuckahoe polysaccharide in a weight ratio of 11:2-4.
8. A method for preparing the maternity-enhancing preparation according to any one of claims 1 to 7, characterized in that: The specific steps include: Weigh the corresponding weights of the raw material components respectively, mix sodium chloride and water to prepare a sodium chloride aqueous solution, and then add the vegetable oil, ginger extract and plant polysaccharide into the sodium chloride aqueous solution respectively to obtain the product.
9. Use of the maternal enhancement preparation according to any one of claims 1 to 7 for preventing animals from cannibalizing their young.
10. A preparation for olfactory training of experimental animals to prevent them from eating their offspring, characterized in that: A preparation for enhancing maternity comprising any one of claims 1 to 7.