Collagen microsphere purification device and use method thereof

Abstract

The invention discloses a collagen microsphere purifying device and a using method thereof, and relates to the field of purifying devices.The collagen microsphere purifying device is characterized by comprising a base, a centrifugal rotating assembly is installed at the top end of the base, and a transparent container is detachably connected to the transmission end of the centrifugal rotating assembly; the purification device for the collagen microspheres further comprises a rotating assembly which is provided with two connecting ends which are respectively connected with a clear liquid collecting box and a sediment collecting tank; the device has the advantages that manual operation is not needed in the whole process, the lifting and storing efficiency can be improved, clear liquid and sediment are separated through the pipettor assembly, and the operation mode that a pipettor adsorbs the clear liquid is relatively mild. When the pipettor is used, the suction head can be slowly and stably close to the surface of supernate, then the supernate is adsorbed by relatively uniform suction force, the disturbance generated to precipitates below in the whole process is extremely small, and the re-suspension of the precipitates is avoided to a greater extent.

Description

Technical Field

[0001] The present invention relates to the field of purification devices, and more specifically, to a purification device for collagen microspheres and a method for using the same. Background Art

[0002] Collagen microspheres are a type of collagen with a spherical structure, which has important application value in many fields such as biomedicine and tissue engineering. As a protein widely present in the human body, collagen has good biocompatibility, degradability and low immunogenicity, which also give collagen microspheres many advantages.

[0003] Most of the conventional crude purification devices for collagen microspheres rely on centrifugation to achieve the purpose of purification. When the collagen microsphere solution is placed in a centrifuge and the centrifugal operation is completed, the staff needs to carefully take out the centrifuge tube (similar to the transparent container in the present invention) from the centrifuge and manually pour out the clear liquid. Since the solution has been stratified at this time, the upper layer is the clear liquid and the lower layer is the collagen microsphere precipitate, if you are not careful, it may cause the solution to shake more and cause the precipitate to re-suspend, affecting the purification effect (a lot of time is wasted here). Later, it is necessary to take out the collagen microsphere precipitate in an appropriate manner. The operation is relatively cumbersome and is manually operated, which makes it impossible to quickly and efficiently purify the collagen microspheres, which seriously restricts the overall efficiency of the purification and is not conducive to meeting the needs of related scientific research or production for rapid purification of collagen microspheres.

[0004] Therefore, in order to solve the above technical problems, the present application proposes a collagen microsphere purification device and a method of using the same. Summary of the invention

[0005] In view of the deficiencies in the prior art, the object of the present invention is to provide a collagen microsphere purification device and a method for using the same.

[0006] To achieve the above object, the present invention provides the following technical solution: a collagen microsphere purification device, comprising a base, a centrifugal rotating assembly is installed on the top of the base, a transparent container is detachably connected to the transmission end of the centrifugal rotating assembly, and the collagen microsphere purification device also includes: The rotating assembly is mounted on the top of the base and has two connecting ends, which are respectively connected to the clear liquid collection box and the sediment collection tank, so that the two are alternately moved to the top of the centrifugal rotating assembly by rotation; The lifting assembly is also installed on the top of the base and is used to drive the pipette assembly to move up and down, and the pipette assembly and the transparent container are located on the same vertical line.

[0007] Preferably, the centrifugal rotating assembly includes a disc fixed on the top of the base, a motor A is installed at the center of the top of the disc, a main gear is fixedly connected to the transmission part of the motor A, and a plurality of driven gears are meshed and connected on the outer wall of the main gear, a plurality of bearings A are fixedly connected to the top of the disc, a rotating rod A is fixedly connected to the inner ring of the bearing A, and the head of the rotating rod A is fixed to the bottom center of the driven gear, and a transparent container is detachably connected to the top of the driven gear.

[0008] Preferably, the rotating assembly includes a motor C fixed to the top of the base, a rotating rod B is fixedly connected to the transmission end of the motor C, and the head of the rotating rod B is respectively fixed to the clear liquid collection box and the sediment collection tank through two connecting rods.

[0009] Preferably, a bearing B is sleeved on the outer wall of the rotating rod B, and the inner ring of the bearing B is fixed to the outer wall of the rotating rod B. The bearing B is fixed to the top of the base through an L-shaped plate, so that the rotating rod B rotates more stably.

[0010] Preferably, the lifting assembly includes a front opening shell fixed to the top of the base, a screw driven by a motor B is installed inside the front opening shell, and a rod sleeve is threadedly connected to the outer wall of the screw, guide rails A are fixedly connected on both sides of the interior of the front opening shell, and both sides of the rod sleeve are slidably connected in the guide rails A through sliders A, and the surface of the rod sleeve is fixed to the pipette assembly through a cross bar.

[0011] Preferably, the top of the driven gear is detachably connected with a transparent container through a quick-release assembly, and the quick-release assembly includes a connecting groove seat fixed on the top of the driven gear, the connecting groove seat extends along its inner side wall to both ends to form grooves, an insert block is inserted into the interior of the connecting groove seat, the interior of the insert block is hollow, and through grooves are opened on both sides, a bearing C is fixedly connected to the inner bottom wall of the insert block, a rotating rod C is fixedly connected to the inner ring portion of the bearing C, and the head of the rotating rod C passes through the top of the insert block, and a rotating plate is fixedly connected to the outer side wall of the rotating rod C, both sides of the top of the rotating plate are rotatably connected to transmission plates, and the head of the transmission plate is rotatably connected to a moving plate, the front and rear sides of the interior of the connecting groove seat are fixedly connected to guide rails B, both sides of the moving plate are slidably connected in the guide rails B through sliders B, and the surface of the moving plate is fixedly connected with protrusions, and both sides of the top of the insert block are fixed to the transparent container through vertical plates, and a locking structure is installed between the head of the rotating rod C and the top of the insert block, so as to facilitate the rapid installation and removal of the transparent container and the driven gear.

[0012] Preferably, the locking structure includes a top plate fixed to the head of the rotating rod C, the side end of the top plate is fixedly connected with an elastic pressing component for pressing down the locking block, the top end of the plug block is fixedly connected with a locking slot seat, and the number of the locking slot seats is two.

[0013] Preferably, the elastic downward pressure assembly includes a lower opening shell fixed to the side end of the top plate, and the inner sides of the lower opening shell are fixedly connected with guide rails C for sliding sliders C, and a horizontal plate is fixedly connected between the sliders C. A spring is installed at the top of the horizontal plate between the top wall of the lower opening shell, and a vertical rod is fixedly connected to the top of the horizontal plate in front of the spring. An open groove is provided at the top of the lower opening shell for the vertical rod to pass through, and a handle is fixedly connected to the top of the vertical rod, and the bottom end of the horizontal plate is fixed to a locking block.

[0014] The method of using the above collagen microsphere purification device comprises the following steps: Step 1: Fill equal amounts of collagen microsphere solution into multiple transparent containers, cover them with top covers after filling, and centrifuge them with a centrifugal rotating assembly; Step 2: After centrifugation, the transparent container will be layered, with the upper layer being the clear liquid and the lower layer being the collagen microsphere precipitate. Then, open the top cover of the transparent container, and lower the pipette assembly through the lifting assembly so that its aspirating parts enter the transparent container respectively, and first aspirate the clear liquid; Step 3: After the clear liquid is sucked, the pipette assembly is moved upward by the lifting assembly. After the upward movement is completed, the clear liquid collection box is moved to the top of the centrifugal rotating assembly by the rotating assembly. At this time, it is just opposite to the transparent container, and the sucked clear liquid can be discharged into the clear liquid collection box for collection; Step 4: After the collection is completed, the clear liquid collection box is rotated to another position by the rotating assembly, and then the pipette assembly is moved downward by the lifting assembly, so as to enter the transparent container again to absorb the precipitate; Step 5: After the collagen microsphere precipitate is absorbed, the pipette assembly is moved upward, and then the precipitate collection tank is rotated to the bottom of the pipette assembly by rotating the assembly, so as to discharge the collagen microsphere precipitate into the precipitate collection tank.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The whole process of the present invention does not require manual operation, which can improve the efficiency of extraction and storage. In addition, the clear liquid and the precipitate are separated by the pipette assembly, and the adsorption of the clear liquid by the pipette is a relatively gentle operation method. When using a pipette, the tip can slowly and steadily approach the surface of the supernatant, and then adsorb the clear liquid with a relatively uniform suction force. The whole process causes minimal disturbance to the precipitate below, and avoids the re-suspension of the precipitate to a greater extent, so as to solve the problem in the background technology that the staff needs to carefully remove the centrifuge tube from the centrifuge and manually pour out the clear liquid. At this time, because the solution has been separated into layers, the upper layer is the clear liquid, and the lower layer is the collagen microsphere precipitate. If you are not careful, it may cause the solution to shake more and re-suspend the precipitate. In addition, the operation is relatively cumbersome and is manually operated, which makes it impossible to quickly and efficiently complete the purification of collagen microspheres. 2. In the present invention, when the rotating rod B rotates, it drives the inner ring of the bearing B to rotate, and the bearing B rotates along its outer ring, so that the rotating rod B is rotationally supported by the bearing B, making its rotation more stable, and also sharing the pressure for the motor C to avoid damage to it; 3. The present invention designs a quick-release structure between the driven gear and the transparent container, which facilitates the rapid installation and removal of the transparent container and the driven gear, and helps to further improve the purification efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings: Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 For the present invention Figure 1 A local structure enlarged view; Figure 3 It is a schematic diagram of the top specific structure of the present invention; Figure 4 It is a schematic diagram of the connection structure of the driven gear in the present invention; Figure 5 This is a schematic diagram of the plug-in block connection structure in the present invention; Figure 6 It is a schematic diagram of the internal structure of the plug block in the present invention; Figure 7 It is a schematic diagram of the internal structure of the lower opening shell of the present invention.

[0017] In the figure: 1, base; 2, transparent container; 3, pipette assembly; 4, rotating assembly; 401, motor C; 402, rotating rod B; 403, connecting rod; 404, bearing B; 405, L-shaped plate; 5, clear liquid collection box; 6, sedimentation collection tank; 7, lifting assembly; 701, front opening shell; 702, motor B; 703, screw rod; 704, guide rail A; 705, slider A; 706, cross bar; 707, rod sleeve; 8, centrifugal rotating assembly; 801, disc; 802, motor A; 803, main gear; 804, driven gear; 805, bearing A; 806, rotating rod A; 9, quick release assembly; 901, Connecting slot seat; 902, groove; 903, plug block; 904, bearing C; 905, rotating rod C; 906, rotating plate; 907, transmission plate; 908, moving plate; 909, guide rail B; 910, protrusion; 911, through groove; 912, slider B; 913, vertical plate; 10, locking structure; 1001, top plate; 1002, locking block; 1003, pressing assembly; 10031, lower opening shell; 10032, guide rail C; 10033, slider C; 10034, horizontal plate; 10035, spring; 10036, vertical rod; 10037, opening slot; 10038, handle; 1004, locking slot seat. DETAILED DESCRIPTION

[0018] Example 1 like Figure 1 and Figure 2 As shown, the present invention provides a collagen microsphere purification device, comprising a base 1, a centrifugal rotating component 8 is installed on the top of the base 1, and a transparent container 2 is detachably connected to the transmission end of the centrifugal rotating component 8. The collagen microsphere purification device also includes: The rotating assembly 4 is mounted on the top of the base 1 and has two connecting ends, which are respectively connected to the clear liquid collection box 5 and the sediment collection tank 6, so that the two are alternately moved to the top of the centrifugal rotating assembly 8 by rotation; The lifting assembly 7 is also installed on the top of the base 1 and is used to drive the pipette assembly 3 to move up and down, and the pipette assembly 3 and the transparent container 2 are located on the same vertical line.

[0019] It should be noted that the pipette assembly 3 in the present invention is composed of a plurality of pipettes, and each pipette is opposite to a transparent container 2. When in use, an equal amount of collagen microsphere solution (the collagen microsphere content therein is also approximately the same) is loaded into the plurality of transparent containers 2. After loading, the top cover is covered, and the centrifugal rotation assembly 8 is used to centrifuge the transparent container 2. After centrifugation, the transparent container 2 will be layered, with the upper layer being a clear liquid and the lower layer being a collagen microsphere precipitate. Then, the top cover of the transparent container 2 is opened, and the pipette assembly 3 is moved downward by the lifting assembly 7 so that its liquid absorption parts enter the transparent container 2 respectively, and the clear liquid is first absorbed (it should be noted here that since the collagen microsphere solution is of equal amount and the collagen microsphere content therein is approximately the same, the clear liquid height and the precipitation height formed in the transparent container 2 are also approximately the same, but there will be some errors. Since the plurality of pipettes are kept lifted uniformly, in order not to absorb the precipitate by mistake, the clear liquid in each transparent container cannot be completely discharged. A small amount of clear liquid residue can be removed in the later production The main purpose of the present invention is to quickly separate most of the clear liquid from the collagen microsphere precipitate). After the absorption is completed, the pipette component 3 is moved up by the lifting component 7. After the upward movement is completed, the clear liquid collection box 5 is moved to the top of the centrifugal rotating component 8 by the rotating component 4. At this time, it is just opposite to the transparent container 2, and the sucked clear liquid can be discharged into the clear liquid collection box 5 for collection. After the collection is completed, the clear liquid collection box 5 is rotated to other parts by the rotating component 4, and the pipette component 3 is moved downward by the lifting component 7, so as to enter the transparent container 2 again to absorb the precipitate. After the absorption is completed, the pipette component 3 is moved up again, and then the precipitate collection tank 6 is rotated to the bottom of the pipette component 3 (the pipette in the pipette component 3 is inhaled and discharged from one port) by the rotating component 4, so that the collagen microsphere precipitate is discharged into the precipitate collection tank 6. The whole process does not require manual operation, which can improve the efficiency of extraction and storage. Moreover, the clear liquid and the precipitate are separated by the pipette component 3, and the pipette adsorbs the clear liquid in a relatively gentle operation mode. When using a pipette, the tip can be slowly and steadily brought close to the surface of the supernatant, and then absorb the clear liquid with a relatively uniform suction force. The whole process causes minimal disturbance to the precipitate below, thus avoiding re-suspension of the precipitate to a greater extent.

[0020] Example 2 like Figures 1 to 3 As shown, this embodiment describes in detail the specific structures of the centrifugal rotating assembly 8, the rotating assembly 4, and the lifting assembly 7 in Embodiment 1: The centrifugal rotating assembly 8 includes a disc 801 fixed on the top of the base 1, a motor A802 is installed at the top center of the disc 801, a main gear 803 is fixedly connected to the transmission part of the motor A802, and a plurality of driven gears 804 are meshed and connected on the outer wall of the main gear 803, a plurality of bearings A805 are fixedly connected to the top of the disc 801, a rotating rod A806 is fixedly connected to the inner ring of the bearing A805, and the head of the rotating rod A806 is fixed to the bottom center of the driven gear 804, and the top of the driven gear 804 is detachably connected to a transparent container 2.

[0021] That is, equal amounts of collagen microsphere solution are loaded into multiple transparent containers 2, and after loading, the top covers are closed, and the transparent containers 2 are installed on the driven gears 804 respectively. During centrifugation, the motor A802 drives the main gear 803 to rotate, the main gear 803 drives the driven gear 804 to rotate, the driven gear 804 drives the rotating rod A806 to rotate, the rotating rod A806 drives the inner ring of the bearing A805 to rotate, and the inner ring of the bearing A805 rotates along its outer ring, thereby rotatably supporting the driven gear 804, and the driven gear 804 drives the self-rotation of multiple transparent containers 2, thereby performing centrifugal rotation.

[0022] The rotating assembly 4 includes a motor C401 fixed on the top of the base 1, and a rotating rod B402 is fixedly connected to the transmission end of the motor C401. The head of the rotating rod B402 is respectively fixed to the clear liquid collecting box 5 and the sediment collecting tank 6 through two connecting rods 403, that is, the motor C401 drives the rotating rod B402 to rotate, and the rotating rod B402 drives the clear liquid collecting box 5 and the sediment collecting tank 6 to rotate with the rotating rod B402 as the center through the connecting rod 403, thereby alternately moving the clear liquid collecting box 5 and the sediment collecting tank 6 to the top of the centrifugal rotating assembly 8, that is, to the position flush with the pipette assembly 3.

[0023] Furthermore, a bearing B404 is sleeved on the outer wall of the rotating rod B402, and the inner ring of the bearing B404 is fixed to the outer wall of the rotating rod B402. The bearing B404 is fixed to the top of the base 1 through the L-shaped plate 405, that is, when the rotating rod B402 rotates, it will drive the inner ring of the bearing B404 to rotate, and the bearing B404 rotates along its outer ring, so that the rotating rod B402 is rotationally supported by the bearing B404, making its rotation more stable, and can also share the pressure for the motor C401 to prevent it from being damaged.

[0024] The lifting assembly 7 includes a front opening shell 701 fixed to the top of the base 1, a screw rod 703 driven by a motor B702 is installed inside the front opening shell 701, and a rod sleeve 707 is threadedly connected to the outer wall of the screw rod 703, and the interior of the front opening shell 701 is fixedly connected to guide rails A704 on both sides, and the two sides of the rod sleeve 707 are slidably connected in the guide rails A704 through sliders A705, and the surface of the rod sleeve 707 is fixed to the pipette assembly 3 through a cross bar 706.

[0025] When in use, the motor B702 drives the screw rod 703 to rotate clockwise or counterclockwise, the screw rod 703 drives the rod sleeve 707 to move up and down, the rod sleeve 707 drives the slider A705 to move up and down, the slider A705 slides along the guide rail A704 to maintain the up and down linear movement of the rod sleeve 707, and the rod sleeve 707 can drive the up and down linear movement of the pipette assembly 3 through the cross bar 706 so as to be used in conjunction with other components.

[0026] Example 3 like Figures 3 to 7 As shown, in this example, a quick-release structure is designed between the driven gear 804 and the transparent container 2, which facilitates the rapid installation and removal of the transparent container 2 and the driven gear 804, and helps to further improve the purification efficiency. The specific structure of the quick-release assembly 9 is as follows: The top of the driven gear 804 is detachably connected to the transparent container 2 through a quick-release assembly 9. The quick-release assembly 9 includes a connecting slot seat 901 fixed to the top of the driven gear 804. The connecting slot seat 901 extends along its inner side wall to both ends to form grooves 902. An insert block 903 is inserted into the interior of the connecting slot seat 901. The interior of the insert block 903 is hollow, and through grooves 911 are opened on both sides. A bearing C904 is fixedly connected to the inner bottom wall of the insert block 903. A rotating rod C905 is fixedly connected to the inner ring of the bearing C904, and the head of the rotating rod C905 passes through the top of the insert block 903. The outer wall of the rotating rod C905 is fixedly connected with a rotating plate 906, and the top sides of the rotating plate 906 are rotatably connected with transmission plates 907, and the head of the transmission plate 907 is rotatably connected with a moving plate 908. The front and rear sides of the interior of the connecting groove seat 901 are fixedly connected with guide rails B909, and the two sides of the moving plate 908 are slidably connected in the guide rails B909 through sliders B912. The surface of the moving plate 908 is fixedly connected with a protrusion 910, and the top sides of the plug block 903 are fixed to the transparent container 2 through vertical plates 913. The head of the rotating rod C905 and the top of the plug block 903 are fixedly connected. A locking structure 10 is installed between the ends (it should be noted that the quick-release assembly 9 includes the locking structure 10), the locking structure 10 includes a top plate 1001 fixed to the head of the rotating rod C905, the side end of the top plate 1001 is fixedly connected to an elastic pressing assembly 1003 for pressing down the locking block 1002, the top end of the plug block 903 is fixedly connected to a locking groove seat 1004, and the number of the locking groove seats 1004 is two, the elastic pressing assembly 1003 includes a lower opening shell 10031 fixed to the side end of the top plate 1001, and the inner sides of the lower opening shell 10031 are fixedly connected to the slider C The guide rail C10032 for sliding 10033, a horizontal plate 10034 is fixedly connected between the slider C10033, a spring 10035 is installed between the top of the horizontal plate 10034 and the inner top wall of the lower opening shell 10031, the top of the horizontal plate 10034 is located in front of the spring 10035 and is fixedly connected with a vertical rod 10036, the top of the lower opening shell 10031 is provided with an open groove 10037 for the vertical rod 10036 to pass through, the top of the vertical rod 10036 is fixedly connected with a handle 10038, and the bottom end of the horizontal plate 10034 is fixed to the locking block 1002.

[0027] In the initial state: the lock block 1002 is inserted into one of the lock slots 1004, and the protrusion 910 is located inside the insert block 903. When the insert block 903 is inserted into the connection slot 901, the handle 10038 can be used to pull the vertical rod 10036 upward, and the vertical rod 10036 drives the horizontal plate 10034 to move upward, and the horizontal plate 10034 drives the slider C10033 to move upward, and the slider C10033 slides upward along the guide rail C10032 to maintain the linear movement of the horizontal plate 10034, thereby squeezing the horizontal plate 10034 and the slider C10033 upward. The spring 10035 before the lower opening shell 10031 pulls the lock block 1002 out of the initial lock slot seat 1004, thereby releasing the lock of the rotating rod C905, thereby rotating the rotating rod C905 and aligning the lock block 1002 with another lock slot seat 1004. In this process, the rotating rod C905 drives the rotation of the rotating plate 906, the rotating plate 906 drives the rotation of the transmission plate 907, the transmission plate 907 drives the two moving plates 908 to move to both sides, the moving plate 908 drives the movement of the guide rail B909, and the guide rail B909 moves along the slider B912 The movable plate 908 moves in a straight line, and the movable plate 908 drives the protrusion 910 to move to both sides, so as to pass through the through slot 911 on the insert block 903 and enter the grooves 902 on both sides of the connecting slot seat 901, and fix the insert block 903 in the connecting slot seat 901, that is, the transparent container 2 is installed on the driven gear 804. At this time, the handle 10038 is released, and the spring 10035 begins to extend in the opposite direction by its elasticity, thereby pressing the locking block 1002 downward, allowing it to enter another locking slot seat 1004, and restricting the rotating rod C905 again. Rotation to avoid affecting the fixation between the plug block 903 and the connecting groove seat 901. Similarly, repeat the above operation, pull up the locking block 1002 again through the handle 10038, and then rotate the rotating rod C905 in the opposite direction to align the locking block 1002 with the initial locking groove seat 1004 again. At this time, the movable plate 908 also drives the protrusion 910 to move toward the middle part, thereby entering the plug block 903, and the fixation between the plug block 903 and the connecting groove seat 901 can be released. The plug block 903 only needs to be pulled off from the connecting groove seat 901 to complete the disassembly of the transparent container 2.

[0028] The present invention also provides a method for using the collagen microsphere purification device: Step 1: Fill equal amounts of collagen microsphere solution into multiple transparent containers 2, cover them with top covers after filling, and centrifuge them with a centrifugal rotating assembly 8; Step 2: After centrifugation, the transparent container 2 will be layered, with the upper layer being the clear liquid and the lower layer being the collagen microsphere precipitate. Then, the top cover of the transparent container 2 is opened, and the pipette component 3 is moved downward by the lifting component 7 so that its liquid aspiration parts enter the transparent container 2 respectively, and the clear liquid is aspirated first; Step 3: After the clear liquid is sucked, the pipette assembly 3 is moved upward by the lifting assembly 7. After the upward movement is completed, the clear liquid collection box 5 is moved to the top of the centrifugal rotating assembly 8 by the rotating assembly 4. At this time, it is just opposite to the transparent container 2, and the sucked clear liquid can be discharged into the clear liquid collection box 5 for collection; Step 4: After the collection is completed, the clear liquid collection box 5 is rotated to another position by the rotating assembly 4, and then the pipette assembly 3 is moved downward by the lifting assembly 7, so as to enter the transparent container 2 again to absorb the sediment; Step 5: After the collagen microsphere precipitate is absorbed, the pipette component 3 is moved upward, and then the precipitate collection tank 6 is rotated to the bottom of the pipette component 3 by the rotating component 4, so that the collagen microsphere precipitate is discharged into the precipitate collection tank 6.

[0029] The collagen microsphere purification device and the use method thereof of the present invention have the following advantages: The process does not require manual operation, which can improve the efficiency of extraction and storage. In addition, the clear liquid and the precipitate are separated by the pipette component 3, and the adsorption of the clear liquid by the pipette is a relatively gentle operation method. When using a pipette, the tip can slowly and steadily approach the surface of the supernatant, and then adsorb the clear liquid with a relatively uniform suction force. The whole process causes minimal disturbance to the precipitate below, and avoids the re-suspension of the precipitate to a greater extent, so as to solve the problem in the background technology that the staff needs to carefully remove the centrifuge tube from the centrifuge and manually pour out the clear liquid. Since the solution has been separated into layers at this time, the upper layer is the clear liquid and the lower layer is the collagen microsphere precipitate. If you are not careful, the solution may shake more and the precipitate may be re-suspended. In addition, the operation is relatively cumbersome and is manually operated, which makes it impossible to quickly and efficiently complete the purification of collagen microspheres. When the rotating rod B402 rotates, it drives the inner ring of the bearing B404 to rotate, and the bearing B404 rotates along its outer ring, so that the rotating rod B402 is supported by the bearing B404 for rotation, making its rotation more stable, and also sharing the pressure for the motor C401 to prevent it from being damaged; A quick-release structure is designed between the driven gear 804 and the transparent container 2 to facilitate quick installation and removal of the transparent container 2 and the driven gear 804, which helps to further improve the purification efficiency.

[0030] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any ordinary technician in the industry can smoothly implement the present invention as shown in the drawings and described above. However, any equivalent changes, modifications and evolutions made by technicians familiar with the profession without departing from the scope of the technical solution of the present invention using the technical content disclosed above are all equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the protection scope of the technical solution of the present invention.

Claims

1. A collagen microsphere purification device, comprising a base (1), characterized in that: A centrifugal rotating assembly (8) is installed at the top of the base (1), and a transparent container (2) is detachably connected to the transmission end of the centrifugal rotating assembly (8). The collagen microsphere purification device also includes: A rotating assembly (4) is mounted on the top of the base (1) and has two connection ends, which are respectively connected to a clear liquid collection box (5) and a sediment collection tank (6), so that the two can be alternately moved to the top of the centrifugal rotating assembly (8) by rotation; The lifting assembly (7) is also mounted on the top of the base (1) and is used to drive the pipette assembly (3) to move up and down, and the pipette assembly (3) and the transparent container (2) are located on the same vertical line.

2. A collagen microsphere purification device according to claim 1, characterized in that: The centrifugal rotating assembly (8) comprises a disc (801) fixed to the top of a base (1); a motor A (802) is installed at the center of the top of the disc (801); a main gear (803) is fixedly connected to the transmission part of the motor A (802); and a plurality of driven gears (804) are meshedly connected on the outer wall of the main gear (803); a plurality of bearings A (805) are fixedly connected to the top of the disc (801); a rotating rod A (806) is fixedly connected to the inner ring of the bearing A (805); and the head of the rotating rod A (806) is fixed to the bottom center of the driven gear (804); and a transparent container (2) is detachably connected to the top of the driven gear (804).

3. The collagen microsphere purification device according to claim 1, characterized in that: The rotating assembly (4) comprises a motor C (401) fixed to the top of the base (1); a rotating rod B (402) is fixedly connected to the driving end of the motor C (401); and the head of the rotating rod B (402) is respectively fixed to the clear liquid collection box (5) and the sediment collection tank (6) via two connecting rods (403).

4. The collagen microsphere purification device according to claim 1, characterized in that: A bearing B (404) is sleeved on the outer wall of the rotating rod B (402), and the inner ring of the bearing B (404) is fixed to the outer wall of the rotating rod B (402). The bearing B (404) is fixed to the top end of the base (1) via an L-shaped plate (405).

5. The collagen microsphere purification device according to claim 1, characterized in that: The lifting assembly (7) comprises a front opening shell (701) fixed to the top of the base (1), a screw rod (703) driven by a motor B (702) is installed inside the front opening shell (701), and a rod sleeve (707) is threadedly connected to the outer wall of the screw rod (703), and the interior of the front opening shell (701) is fixedly connected to guide rails A (704) on both sides, and the two sides of the rod sleeve (707) are slidably connected to the guide rails A (704) through sliders A (705), and the surface of the rod sleeve (707) is fixed to the pipette assembly (3) through a cross bar (706).

6. The collagen microsphere purification device according to claim 1, characterized in that: The top end of the driven gear (804) is detachably connected to a transparent container (2) via a quick-release assembly (9). The quick-release assembly (9) comprises a connecting groove seat (901) fixed to the top end of the driven gear (804). The connecting groove seat (901) extends along its inner side wall to both ends to form grooves (902). An insert block (903) is inserted into the interior of the connecting groove seat (901). The interior of the insert block (903) is hollow and has through grooves (911) on both sides. A bearing C (904) is fixedly connected to the inner bottom wall of the insert block (903). A rotating rod C (905) is fixedly connected to the inner ring of the bearing C (904). The head of the rotating rod C (905) passes through the top of the insert block (903). The rotating rod C (905) is fixedly connected to the inner ring of the bearing C (904). A rotating plate (906) is fixedly connected to the outer wall of the connecting groove seat (905), the top sides of the rotating plate (906) are rotatably connected to transmission plates (907), the head of the transmission plate (907) is rotatably connected to a moving plate (908), the front and rear sides of the interior of the connecting groove seat (901) are fixedly connected to guide rails B (909), the two sides of the moving plate (908) are slidably connected in the guide rails B (909) through sliders B (912), the surface of the moving plate (908) is fixedly connected to a protrusion (910), the top sides of the insert block (903) are fixed to the transparent container (2) through vertical plates (913), and a locking structure (10) is installed between the head of the rotating rod C (905) and the top of the insert block (903).

7. The collagen microsphere purification device according to claim 6, characterized in that: The locking structure (10) comprises a top plate (1001) fixed to the head of a rotating rod C (905); a side end of the top plate (1001) is fixedly connected to an elastic pressing component (1003) for pressing down a locking block (1002); a top end of the insert block (903) is fixedly connected to a locking slot seat (1004), and the number of the locking slot seats (1004) is two.

8. The collagen microsphere purification device according to claim 7, characterized in that: The elastic downward pressing component (1003) comprises a lower opening shell (10031) fixed to the side end of the top plate (1001), the inner sides of the lower opening shell (10031) are fixedly connected with guide rails C (10032) for sliding of the slider C (10033), the sliders C (10033) are fixedly connected with a cross plate (10034), and a top end of the cross plate (10034) and an inner top wall of the lower opening shell (10031) are installed. The top end of the horizontal plate (10034) is located in front of the spring (10035) and is fixedly connected to a vertical rod (10036); the top end of the lower opening shell (10031) is provided with an opening slot (10037) for the vertical rod (10036) to pass through; the top end of the vertical rod (10036) is fixedly connected to a handle (10038); and the bottom end of the horizontal plate (10034) is fixed to the locking block (1002).

9. A method for using the collagen microsphere purification device according to any one of claims 1 to 8, characterized in that: The steps include: Step 1: Filling a plurality of transparent containers (2) with equal amounts of collagen microsphere solution, covering the containers with top covers after filling, and centrifugally rotating the containers using a centrifugal rotating assembly (8); Step 2: After centrifugation, the transparent container (2) will be layered, with the upper layer being the clear liquid and the lower layer being the collagen microsphere precipitate. The top cover of the transparent container (2) is then opened, and the pipette component (3) is moved downward by the lifting component (7) so that its liquid aspiration part enters the transparent container (2) to aspirate the clear liquid first. Step 3: After the clear liquid is sucked, the pipette assembly (3) is moved upward by the lifting assembly (7). After the upward movement is completed, the clear liquid collection box (5) is moved to the top of the centrifugal rotating assembly (8) by the rotating assembly (4). At this time, it is just opposite to the transparent container (2), and the sucked clear liquid can be discharged into the clear liquid collection box (5) for collection; Step 4: After the collection is completed, the clear liquid collection box (5) is rotated to another position by the rotating component (4), and then the pipette component (3) is moved downward by the lifting component (7), so as to enter the transparent container (2) again to absorb the precipitate; Step 5: After the collagen microsphere precipitate is absorbed, the pipette component (3) is moved upward, and then the precipitate collection tank (6) is rotated to the bottom of the pipette component (3) by the rotating component (4), so that the collagen microsphere precipitate is discharged into the precipitate collection tank (6).

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