Kitchen oil stain detergent and preparation method thereof

Abstract

The invention provides a kitchen oil stain detergent and a preparation method thereof, and belongs to the technical field of detergents. The preparation method comprises the following steps: preparing porous hollow microspheres with oleophylic inside and hydrophilic outside, adsorbing alkaline lipase and alkaline protease, fermenting the microspheres with Chinese honeylocust fruit, orange peel and lemon peel to obtain a fermentation product, mixing the fermentation product with a wall-broken product prepared from zymophyte through wall breaking, adding isopropanol, water and essence, and uniformly mixing to obtain the kitchen oil stain detergent. The kitchen oil stain detergent prepared by the invention has a very good removal effect on intractable kitchen stains by adopting an organic solvent, surfactant, enzyme and physical adsorption composite treatment method, is high in cleaning speed, good in effect, safe, environment-friendly, mild, low in toxicity, small in irritation, low in cost and good in stability, and has a wide application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of detergents, and in particular to a kitchen greasy dirt detergent and a preparation method thereof. Background Art

[0002] Kitchen dirt is mainly formed by the dripping of cooking oil and the evaporation of the heated part, which contaminates the utensils, and with the addition of dust, a layer of viscous and highly adhesive grease is formed over time. This kind of grease is difficult to remove with ordinary cleaning agents. At present, the commonly used kitchen oil cleaning mainly adopts strong alkaline cleaning and solvent-based cleaning technology. Although both have a high oil removal rate, they are less safe to use and have a greater corrosive and damaging effect on human skin and kitchen facilities. At the same time, if the Zhejiang West detergent is used for household cleaning, its wastewater is discharged into rivers through the sewer, directly polluting the river water, and is used in the breeding industry or planting industry. The surfactants in it are absorbed by these animals and plants. People who eat these contaminated animals and plants for a long time will cause great harm to their bodies.

[0003] Therefore, the development of efficient, safe and environmentally friendly kitchen grease detergent has great application value and development prospects. Summary of the invention

[0004] The purpose of the present invention is to provide a kitchen oil stain detergent and a preparation method thereof. By adopting a composite treatment method of organic solvent + surfactant + enzyme + physical adsorption, the detergent has a good removal effect on stubborn kitchen stains, has a fast cleaning speed, good effect, safety and environmental protection, is mild, has low toxicity, low irritation, low cost, good stability, and has broad application prospects.

[0005] The technical solution of the present invention is achieved in this way:

[0006] The invention provides a method for preparing a kitchen greasy dirt detergent. A porous hollow microsphere which is lipophilic inside and hydrophilic outside is prepared, alkaline lipase and alkaline protease are adsorbed on the microsphere, and then fermented with soapberry, orange peel and lemon peel to obtain a fermentation product. The microsphere is mixed with a broken wall product obtained by breaking the wall of fermented bacteria, and isopropyl alcohol, water and essence are added to mix the mixture evenly to prepare the kitchen greasy dirt detergent.

[0007] As a further improvement of the present invention, the following steps are included:

[0008] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer parts: dropping aminosilane, long-chain alkyl silane and porogen into an aqueous solution containing an emulsifier, emulsifying, stirring for reaction, spray drying, and obtaining porous hollow microspheres with lipophilic inner and hydrophilic outer parts;

[0009] S2. Adsorption of compound enzyme: alkaline lipase and alkaline protease were mixed evenly, added to water, and porous hollow microspheres with lipophilic inner and hydrophilic outer were added, stirred for reaction, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0010] S3. Fermentation: Gleditsia sinensis, orange peel and lemon peel were mixed and crushed, added to water, inoculated with fermentation bacteria, fermented and cultured, filtered, the solid solution was washed and collected, freeze-dried, and a composite bacteria was obtained; the filtrate was dried to obtain a fermentation product;

[0011] S4. Breaking the wall of the bacterial sludge: adding the composite bacteria to water, adding snail enzyme and lysozyme, heating and stirring for enzymolysis, filtering, and drying the filtrate to obtain a broken wall product;

[0012] S5. Preparation of kitchen grease detergent: Evenly mix the immobilized enzyme degreasing microspheres, fermentation products, broken cell products, isopropanol, water and essence, adjust the pH value of the solution, and prepare the kitchen grease detergent.

[0013] As a further improvement of the present invention, the mass ratio of the aminosilane, the long-chain alkyl silane, the porogen and the emulsifier in step S1 is 12-15:10-12:1-2:0.5-1:0.5-1, the aminosilane is selected from at least one of KH550, KH602 and KH792, the long-chain alkyl silane is selected from at least one of dodecyltrimethoxysilane, dodecyltriethoxysilane, dodecylmethyldimethoxysilane, hexadecyltrimethoxysilane and octadecyltrimethoxysilane, the porogen is selected from at least one of hexadecyltrimethylammonium chloride, hexadecyldimethylbenzylammonium chloride and hexadecyltrimethylammonium bromide, the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85, and the stirring reaction time is 5-7h.

[0014] As a further improvement of the present invention, in step S2, the mass ratio of alkaline lipase, alkaline protease and porous hollow microspheres with inner lipophilicity and outer hydrophilicity is 3-4:1-2:12-16, and the stirring reaction time is 2-3h.

[0015] As a further improvement of the present invention, the mass ratio of the sapodilla, orange peel and lemon peel in step S3 is 7-10:4-6:2-4, the fermentation bacteria are Bacillus licheniformis and Bifidobacterium lactis, and the inoculated bacteria are their seed liquids, and the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL, the inoculation amounts were 2-3v / v% and 1-2v / v%, respectively, and the fermentation culture conditions were 39-41°C, 100-200r / min, and the fermentation culture was 36-48h.

[0016] As a further improvement of the present invention, the mass ratio of the composite bacteria, snail enzyme and lysozyme in step S4 is 100:2-3:3-5, the temperature of the heated and stirred enzymolysis is 35-40°C, and the time is 1-3h.

[0017] As a further improvement of the present invention, the mass ratio of the immobilized enzyme deoiling microspheres, fermentation products, broken cell products, ethanol, isopropanol, water and essence in step S5 is 10-15:4-6:3-5:15-25:7-12:100-150:0.5-1, and the pH value of the adjusting solution is 7.3-7.5.

[0018] As a further improvement of the present invention, the present invention specifically comprises the following steps:

[0019] S1. Preparation of porous hollow lipophilic and hydrophilic microspheres: 12-15 parts by weight of aminosilane, 10-12 parts by weight of long-chain alkyl silane, and 1-2 parts by weight of porogen are dropped into an aqueous solution containing 0.5-1 parts by weight of an emulsifier, emulsified, stirred for 5-7h, and spray-dried to obtain porous hollow lipophilic and hydrophilic microspheres;

[0020] S2. Adsorption of the composite enzyme: 3-4 parts by weight of alkaline lipase and 1-2 parts by weight of alkaline protease were mixed uniformly, added to water, 12-16 parts by weight of porous hollow lipophilic and hydrophilic microspheres were added, the reaction was stirred for 2-3h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0021] S3. Fermentation: 7-10 parts by weight of sapodilla, 4-6 parts by weight of orange peel, 2-4 parts by weight of lemon peel were mixed and crushed, added to 200 parts by weight of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amount was 2-3v / v% and 1-2v / v%, 39-41 ° C, 100-200r / min, fermentation culture 36-48h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0022] S4. Broken wall of bacterial sludge: 10 parts by weight of composite bacteria were added to water, 0.2-0.3 parts by weight of snail enzyme and 0.3-0.5 parts by weight of lysozyme were added, heated to 35-40 ° C, stirred for enzymolysis for 1-3h, filtered, and the filtrate was dried to obtain a broken wall product;

[0023] S5. Preparation of kitchen grease detergent: Mix 10-15 parts by weight of immobilized enzyme degreasing microspheres, 4-6 parts by weight of fermentation products, 3-5 parts by weight of broken cell products, 7-12 parts by weight of isopropanol, 100-150 parts by weight of water and 0.5-1 parts by weight of flavor, adjust the pH value of the solution to 7.3-7.5, and prepare a kitchen grease detergent.

[0024] The present invention further protects a kitchen grease detergent prepared by the above preparation method.

[0025] The present invention further protects the use of the above kitchen oil stain detergent in cleaning oil stains and stains.

[0026] The present invention has the following beneficial effects:

[0027] Kitchen grease is generally formed by cross-linking of grease polymers and other dirt, which is difficult to dissolve in water and difficult to be emulsified and solubilized under normal conditions, thus increasing the difficulty of cleaning kitchen grease. The present invention adopts a method of organic solvent + surfactant + enzyme + physical adsorption composite treatment, wherein organic solvents such as isopropanol can effectively clean polymers in grease through wetting, penetration, dissolution and emulsification, but a large amount of organic solvents is prone to cause strong irritation and high storage risk. Therefore, the present invention adds a small amount of isopropanol. The invention does not directly add a surfactant, but can produce a large amount of surfactant substances by fermenting saponins, orange peels and lemon peels under the fermentation action of fermentation bacteria (Bacillus licheniformis and Bifidobacterium lactis), and has the characteristics of being green, high-yield, environmentally friendly and safe. The saponins, orange peels and lemon peels themselves contain rich saponin, saponin phenol, saponin, saponin alcohol, hesperidin, naringin, alkaloids, essential oils and the like, and after fermentation, rich surfactant substances are produced, and the detergent has good anti-oxidation and fragrance effects, thereby improving the stability of the prepared kitchen oil stain detergent.

[0028] In addition, the fermentation bacteria itself can be broken down by the synergistic wall-breaking action of snail enzyme and lysozyme, so that the content is greatly dissolved, which greatly increases the content of surfactants, thereby significantly improving the decontamination rate and oil removal rate. Surfactants can separate oil stains from the surface of objects through wetting, penetration, solubilization and emulsification, and have the characteristics of mildness, low toxicity and low irritation. At the same time, compared with directly adding surfactants, the surfactants produced by the microorganisms of the present invention have the advantages of low cost, good effect, reduced environmental pollution, safety and environmental protection, etc.

[0029] The present invention prepares porous hollow inner lipophilic outer hydrophilic microspheres, aminosilane and long-chain alkylsilane are not initially soluble in water, but are dispersed into small droplets during the stirring and emulsification process, and the amino group is protonated to become an amphiphilic molecule, further stabilizing the silane droplets, and providing an alkaline environment to catalyze the sol-gel reaction process of silane, and the consumption of the internal silane will obtain a hollow ball structure. The long-chain alkyl group will spontaneously face the inside of the shell due to hydrophobicity, so that the outer surface is amino, and the inner surface is a hollow silica ball of long-chain alkyl. Under the action of a porogen, the silica shell presents a porous structure, which increases its specific surface area and provides a rich site for subsequent adsorption and fixation of enzymes. At the same time, since the inner layer of the hollow ball is rich in hydrophobic long-chain alkyl, it can adsorb oil stains into the inside of the hollow ball, and can be enzymolyzed by the composite enzyme loaded thereon, thereby improving the efficiency of degreasing.

[0030] The composite enzyme of the present invention comprises alkaline lipase and alkaline protease, which can improve the decontamination ability of kitchen oil stains, reduce the amount of auxiliaries and surfactants in detergents, and reduce environmental pollution. Kitchen oil stains are mainly formed by the splashing and accumulation of vegetable oil used in daily life. The main component of vegetable oil is triglyceride. Alkaline lipase can hydrolyze triglyceride into fatty acids and glycerol to achieve the effect of cleaning kitchen oil stains. At the same time, some protein mixed stains can also be enzymolyzed together, thereby playing a synergistic effect.

[0031] The kitchen oil stain detergent prepared by the invention has a good removal effect on stubborn kitchen stains by adopting a composite treatment method of organic solvent + surfactant + enzyme + physical adsorption, has a fast cleaning speed, good effect, safety and environmental protection, mildness, low toxicity, low irritation, low cost, good stability, and has broad application prospects. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Bacillus licheniformis, 20 billion cfu / g, was purchased from Guangzhou Weiyuan Biotechnology Co., Ltd. Bifidobacterium lactis, 10 billion cfu / g, was purchased from Qingdao Norsen Biotechnology Co., Ltd.

[0034] Preparation method of bacterial seed liquid: inoculate the bacterial strain into Gao's medium, activate and culture at the optimum temperature, 150r / min, for 18-24h, and obtain a medium with a bacterial content of 10 8 -10 9 cfu / mL of bacterial seed solution.

[0035] Snail enzyme, with a cell wall breaking rate of 90%, was purchased from Shanghai Yuanye Biotechnology Co., Ltd.; lysozyme, 20,000 U / g, was purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.

[0036] Alkaline lipase, 100,000 U / g, and alkaline protease, 50,000 U / g, were purchased from Wuxi Youpu Biotechnology Co., Ltd.

[0037] Example 1

[0038] This embodiment provides a method for preparing a kitchen grease detergent, which specifically comprises the following steps:

[0039] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces: 12 g of silane coupling agent KH550, 10 g of dodecyltrimethoxysilane, and 1 g of hexadecyltrimethylammonium chloride were dropped into 200 mL of an aqueous solution containing 0.5 g of Tween-20, emulsified at 5000 r / min for 15 min, stirred for 5 h, and spray-dried to obtain porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces;

[0040] S2. Adsorption of compound enzyme: 3 g alkaline lipase and 1 g alkaline protease were mixed evenly, added to 100 mL water, 12 g porous hollow microspheres with lipophilic inner and hydrophilic outer were added, stirred for 2 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0041] S3. Fermentation: 7g of sapodilla, 4g of orange peel, 2g of lemon peel were mixed and crushed, added to 200mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 2v / v% and 1v / v%, 39°C, 100r / min, fermented for 36h, filtered, the solid solution was collected by washing, freeze-dried, and a composite bacteria was obtained; the filtrate was dried to obtain a fermentation product;

[0042] S4. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.2 g of snail enzyme and 0.3 g of lysozyme were added, heated to 35 ° C, stirred for enzymolysis for 1 h, filtered, and the filtrate was dried to obtain a broken product;

[0043] S5. Preparation of kitchen grease detergent: 10 g of immobilized enzyme degreasing microspheres, 4 g of fermentation product, 3 g of broken cell wall product, 7 g of isopropanol, 100 mL of water and 0.5 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.3 to prepare a kitchen grease detergent.

[0044] Example 2

[0045] This embodiment provides a method for preparing a kitchen grease detergent, which specifically comprises the following steps:

[0046] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces: 15 g of silane coupling agent KH602, 12 g of dodecylmethyldimethoxysilane, and 2 g of hexadecyldimethylbenzyl ammonium chloride were dropped into 200 mL of an aqueous solution containing 1 g of Tween-40, emulsified at 5000 r / min for 15 min, stirred for 7 h, and spray-dried to obtain porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces;

[0047] S2. Adsorption of compound enzyme: 4 g alkaline lipase and 2 g alkaline protease were mixed evenly, added to 100 mL water, 16 g porous hollow microspheres with lipophilic inner and hydrophilic outer were added, stirred for 3 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0048] S3. Fermentation: 10 g of sapodilla, 6 g of orange peel, 4 g of lemon peel were mixed and crushed, added to 200 mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 3 v / v% and 2 v / v%, 41 ° C, 200 r / min, fermentation culture 48h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0049] S4. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.3 g of snail enzyme and 0.5 g of lysozyme were added, heated to 40 ° C, stirred for enzymatic hydrolysis for 3 h, filtered, and the filtrate was dried to obtain a broken product;

[0050] S5. Preparation of kitchen grease detergent: 15 g of immobilized enzyme degreasing microspheres, 6 g of fermentation product, 5 g of broken cell wall product, 12 g of isopropanol, 150 mL of water and 1 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.5 to prepare a kitchen grease detergent.

[0051] Example 3

[0052] This embodiment provides a method for preparing a kitchen grease detergent, which specifically comprises the following steps:

[0053] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces: 13.5 g of silane coupling agent KH792, 11 g of octadecyltrimethoxysilane, and 1.5 g of hexadecyltrimethylammonium bromide were added to 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for 6 h, and spray-dried to obtain porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces;

[0054] S2. Adsorption of compound enzyme: 3.5 g alkaline lipase and 1.5 g alkaline protease were mixed evenly, added to 100 mL of water, 14 g of porous hollow microspheres with lipophilic inner and hydrophilic outer layers were added, stirred for 2.5 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0055] S3. Fermentation: 8 g of sapodilla, 5 g of orange peel, 3 g of lemon peel were mixed and crushed, added to 200 mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 2.5 v / v% and 1.5 v / v%, 40 ° C, 150 r / min, fermentation culture 42h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0056] S4. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.25 g of snail enzyme and 0.4 g of lysozyme were added, heated to 37 ° C, stirred for enzymolysis for 2 h, filtered, and the filtrate was dried to obtain a broken product;

[0057] S5. Preparation of kitchen grease detergent: 12 g of immobilized enzyme degreasing microspheres, 5 g of fermentation product, 4 g of broken cell wall product, 10 g of isopropanol, 125 mL of water and 0.7 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0058] Comparative Example 1

[0059] Compared with Example 3, the difference is that the porogen hexadecyltrimethylammonium bromide is not added in step S1.

[0060] The details are as follows:

[0061] S1. Preparation of hollow microspheres with lipophilic inner part and hydrophilic outer part: 13.5 g of silane coupling agent KH792 and 11 g of octadecyltrimethoxysilane were added into 200 mL of aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for reaction for 6 h, and spray dried to obtain hollow microspheres with lipophilic inner part and hydrophilic outer part.

[0062] Comparative Example 2

[0063] Compared with Example 3, the difference is that octadecyltrimethoxysilane is not added in step S1.

[0064] The details are as follows:

[0065] S1. Preparation of porous hollow microspheres: 24.5 g of silane coupling agent KH792 and 1.5 g of hexadecyltrimethylammonium bromide were added to 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for reaction for 6 h, and spray-dried to obtain porous hollow microspheres.

[0066] Comparative Example 3

[0067] Compared with Example 3, the difference is that alkaline lipase is not added in step S2.

[0068] The details are as follows:

[0069] S2. Adsorption of composite enzyme: 5 g alkaline protease was mixed evenly, added into 100 mL water, and 14 g porous hollow microspheres with lipophilic inner part and hydrophilic outer part were added, stirred for reaction for 2.5 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres.

[0070] Comparative Example 4

[0071] Compared with Example 3, the difference is that alkaline protease is not added in step S2.

[0072] The details are as follows:

[0073] S2. Adsorption of composite enzyme: 5 g alkaline lipase was mixed evenly, added into 100 mL water, and 14 g porous hollow microspheres with lipophilic inner part and hydrophilic outer part were added, stirred for reaction for 2.5 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres.

[0074] Comparative Example 5

[0075] Compared with Example 3, the difference is that step S2 is not performed.

[0076] The details are as follows:

[0077] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces: 13.5 g of silane coupling agent KH792, 11 g of octadecyltrimethoxysilane, and 1.5 g of hexadecyltrimethylammonium bromide were added to 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for 6 h, and spray-dried to obtain porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces;

[0078] S2. Fermentation: 8 g of sapodilla, 5 g of orange peel, 3 g of lemon peel were mixed and crushed, added to 200 mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 2.5 v / v% and 1.5 v / v%, 40 ° C, 150 r / min, fermentation culture 42h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0079] S3. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.25 g of snail enzyme and 0.4 g of lysozyme were added, heated to 37 ° C, stirred for enzymolysis for 2 h, filtered, and the filtrate was dried to obtain a broken product;

[0080] S4. Preparation of kitchen grease detergent: 12g of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces, 5g of fermentation product, 4g of broken wall product, 10g of isopropanol, 125mL of water and 0.7g of bergamot essence were stirred and mixed for 15min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0081] Comparative Example 6

[0082] Compared with Example 3, the difference is that the Bacillus licheniformis seed liquid is not inoculated in step S3.

[0083] The details are as follows:

[0084] S3. Fermentation: Mix and crush 8g of saponin, 5g of orange peel and 3g of lemon peel, add them to 200mL of water, inoculate with 4v / v% of Bifidobacterium lactis seed solution, at 40°C, 150r / min, ferment for 42h, filter, wash the solid and collect the bacterial solution, freeze-dry to obtain a composite bacteria; dry the filtrate to obtain a fermentation product.

[0085] Comparative Example 7

[0086] Compared with Example 3, the difference is that the Bifidobacterium lactis seed liquid is not inoculated in step S3.

[0087] The details are as follows:

[0088] S3. Fermentation: Mix and crush 8g of soap beans, 5g of orange peel and 3g of lemon peel, add them to 200mL of water, inoculate with licheniformis seed solution at an inoculation rate of 4v / v%, 40°C, 150r / min, ferment for 42h, filter, wash the solid and collect the bacterial solution, freeze-dry to obtain a composite bacteria; dry the filtrate to obtain a fermentation product.

[0089] Comparative Example 8

[0090] Compared with Example 3, the difference is that no immobilized enzyme deoiling microspheres are added in step S5.

[0091] The details are as follows:

[0092] S5. Preparation of kitchen grease detergent: 5 g of fermentation product, 4 g of broken cell product, 10 g of isopropanol, 125 mL of water and 0.7 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0093] Comparative Example 9

[0094] Compared with Example 3, the difference is that no fermentation product is added in step S5.

[0095] The details are as follows:

[0096] S5. Preparation of kitchen grease detergent: 12 g of immobilized enzyme degreasing microspheres, 4 g of broken wall product, 10 g of isopropanol, 125 mL of water and 0.7 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0097] Comparative Example 10

[0098] Compared with Example 3, the difference is that no cell wall breaking product is added in step S5.

[0099] The details are as follows:

[0100] S5. Preparation of kitchen grease detergent: 12 g of immobilized enzyme degreasing microspheres, 5 g of fermentation product, 10 g of isopropanol, 125 mL of water and 0.7 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0101] Test Example 1 Determination of biosurfactant production

[0102] The fermentation products and cell wall-breaking products obtained in Examples 1-3 of the present invention and Comparative Examples 6 and 7 were adjusted to a pH of 2.0 with 6 mol / L HCl, extracted twice with a 2:1 mixed solvent of chloroform and methanol, and the organic phases were collected and dried to obtain surfactants, and the yield was calculated. The results are shown in Table 1.

[0103] Yield = mass of surfactant / mass of fermentation product or cell wall-broken product × 100%

[0104] Table 1

[0105]

[0106] It can be seen from the above table that the fermentation products and cell wall-broken products obtained in Examples 1-3 of the present invention contain relatively high contents of surfactants.

[0107] Test Example 2 Determination of Oil Drainage Performance

[0108] Take a culture dish with a diameter of 20 cm, add 50 mL of water, add 2 mL of rapeseed oil in the center, and after the oil film is evenly spread, add 1 drop of kitchen oil detergent prepared in Examples 1-3 and Comparative Examples 1-10 in the center, and measure the diameter of the oil drain ring. The results are shown in Table 2.

[0109] Table 2

[0110]

[0111]

[0112] It can be seen from the above table that the kitchen oily detergents prepared in Examples 1-3 of the present invention have good oil removal performance.

[0113] Test Example 3

[0114] The kitchen grease detergents prepared in Examples 1-3 of the present invention and Comparative Examples 1-10 were tested for their decontamination and degreasing effects. The results are shown in Table 3.

[0115] Decontamination test:

[0116] Clean the glassware with anhydrous ethanol, dry it and weigh it as M1, pour the stain (equal amounts of vegetable oil and lard account for 15%, wheat flour accounts for 15%, whole milk powder accounts for 7%, egg liquid accounts for 30%, and distilled water accounts for 33%) into the plate and soak for 10 seconds, dry it for 30 minutes, scrub the unabsorbed oil on the plate and weigh it again and record it as M2, dilute the kitchen oil detergent 100 times, control the water temperature at (35±2)℃, put the plate in it, stir it twice with a stick, then dry the plate, cool it, weigh it again and record it as M3. The calculation formula is as follows:

[0117] Decontamination rate (%) = (M2-M3) / (M2-M1) × 100%

[0118] Oil removal rate test:

[0119] Clean the glassware with anhydrous ethanol, dry it and weigh it as M1. Pour vegetable oil into the tableware and soak it for 10 seconds, dry it for 30 minutes, wipe off the unabsorbed oil on the tableware with filter paper and weigh it again as M2. Dilute the kitchen oil detergent by 100 times. Add 2500mL to each 5L plastic bucket, control the water temperature at (35±2)℃, soak the tableware with oil stains in the detergent solution and shake or stir it slightly for 30 seconds, then dry the tableware, cool it and weigh it, record it as M3, and calculate the oil removal rate.

[0120] Oil removal rate (%) = (M2-M3) / (M2-M1) × 100%

[0121] Table 3

[0122] Group Decontamination rate (%) Oil removal rate (%) Example 1 97.8 98.9 Example 2 97.4 99.2 Example 3 98.0 99.5 Comparative Example 1 93.9 94.1 Comparative Example 2 95.6 92.8 Comparative Example 3 94.9 90.7 Comparative Example 4 90.8 93.9 Comparative Example 5 88.7 87.2 Comparative Example 6 91.1 92.4 Comparative Example 7 90.7 91.1 Comparative Example 8 84.1 81.7 Comparative Example 9 86.9 87.2 Comparative Example 10 85.5 84.9

[0123] It can be seen from the above table that the kitchen oily detergents prepared in Examples 1-3 of the present invention have good decontamination and degreasing performance.

[0124] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a kitchen grease detergent, characterized in that: A porous hollow microsphere with lipophilic inner part and hydrophilic outer part is prepared, alkaline lipase and alkaline protease are adsorbed on the microsphere, and then the microsphere is mixed with saponin, orange peel and lemon peel to obtain a fermentation product and a broken wall product obtained by breaking the wall of fermented bacteria, and then isopropyl alcohol, water and essence are added and mixed evenly to obtain a kitchen grease detergent.

2. The preparation method according to claim 1, characterized in that: The following steps are involved: S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer parts: dropping aminosilane, long-chain alkyl silane and porogen into an aqueous solution containing an emulsifier, emulsifying, stirring for reaction, spray drying, and obtaining porous hollow microspheres with lipophilic inner and hydrophilic outer parts; S2. Adsorption of compound enzyme: alkaline lipase and alkaline protease were mixed evenly, added to water, and porous hollow microspheres with lipophilic inner and hydrophilic outer were added, stirred for reaction, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres; S3. Fermentation: Gleditsia sinensis, orange peel and lemon peel were mixed and crushed, added to water, inoculated with fermentation bacteria, fermented and cultured, filtered, the solid solution was washed and collected, freeze-dried, and a composite bacteria was obtained; the filtrate was dried to obtain a fermentation product; S4. Breaking the wall of the bacterial sludge: adding the composite bacteria to water, adding snail enzyme and lysozyme, heating and stirring for enzymolysis, filtering, and drying the filtrate to obtain a broken wall product; S5. Preparation of kitchen grease detergent: Evenly mix the immobilized enzyme degreasing microspheres, fermentation products, broken cell products, isopropanol, water and essence, adjust the pH value of the solution, and prepare the kitchen grease detergent.

3. The preparation method according to claim 2, characterized in that: The mass ratio of the aminosilane, long-chain alkylsilane, porogen and emulsifier in step S1 is 12-15:10-12:1-2:0.5-1:0.5-1, the aminosilane is selected from at least one of KH550, KH602 and KH792, the long-chain alkylsilane is selected from at least one of dodecyltrimethoxysilane, dodecyltriethoxysilane, dodecylmethyldimethoxysilane, hexadecyltrimethoxysilane and octadecyltrimethoxysilane, the porogen is selected from at least one of hexadecyltrimethylammonium chloride, hexadecyldimethylbenzylammonium chloride and hexadecyltrimethylammonium bromide, the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85, and the stirring reaction time is 5-7h.

4. The preparation method according to claim 2, characterized in that: In step S2, the mass ratio of alkaline lipase, alkaline protease and porous hollow microspheres with lipophilic inner part and hydrophilic outer part is 3-4:1-2:12-16, and the stirring reaction time is 2-3h.

5. The preparation method according to claim 2, characterized in that: In step S3, the mass ratio of sapodilla, orange peel and lemon peel is 7-10:4-6:2-4, the fermentation bacteria are Bacillus licheniformis and Bifidobacterium lactis, and the inoculated bacteria are their seed liquid, and the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL, the inoculation amounts were 2-3v / v% and 1-2v / v%, respectively, and the fermentation culture conditions were 39-41°C, 100-200r / min, and the fermentation culture was 36-48h.

6. The preparation method according to claim 2, characterized in that: In step S4, the mass ratio of the composite bacteria, snail enzyme and lysozyme is 100:2-3:3-5, and the temperature of the heated stirring enzymolysis is 35-40° C. and the time is 1-3 h.

7. The preparation method according to claim 2, characterized in that: In step S5, the mass ratio of the immobilized enzyme deoiling microspheres, fermentation products, broken cell products, ethanol, isopropanol, water and essence is 10-15:4-6:3-5:15-25:7-12:100-150:0.5-1, and the pH value of the adjustment solution is 7.3-7.

5.

8. The preparation method according to claim 2, characterized in that: The specific steps include: S1. Preparation of porous hollow lipophilic and hydrophilic microspheres: 12-15 parts by weight of aminosilane, 10-12 parts by weight of long-chain alkyl silane, and 1-2 parts by weight of porogen are dropped into an aqueous solution containing 0.5-1 parts by weight of an emulsifier, emulsified, stirred for 5-7h, and spray-dried to obtain porous hollow lipophilic and hydrophilic microspheres; S2. Adsorption of the composite enzyme: 3-4 parts by weight of alkaline lipase and 1-2 parts by weight of alkaline protease were mixed uniformly, added to water, 12-16 parts by weight of porous hollow lipophilic and hydrophilic microspheres were added, the reaction was stirred for 2-3h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres; S3. Fermentation: 7-10 parts by weight of sapodilla, 4-6 parts by weight of orange peel, 2-4 parts by weight of lemon peel were mixed and crushed, added to 200 parts by weight of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amount was 2-3v / v% and 1-2v / v%, 39-41 ° C, 100-200r / min, fermentation culture 36-48h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product; S4. Broken wall of bacterial sludge: 10 parts by weight of composite bacteria were added to water, 0.2-0.3 parts by weight of snail enzyme and 0.3-0.5 parts by weight of lysozyme were added, heated to 35-40 ° C, stirred for enzymolysis for 1-3h, filtered, and the filtrate was dried to obtain a broken wall product; S5. Preparation of kitchen grease detergent: Mix 10-15 parts by weight of immobilized enzyme degreasing microspheres, 4-6 parts by weight of fermentation products, 3-5 parts by weight of broken cell products, 7-12 parts by weight of isopropanol, 100-150 parts by weight of water and 0.5-1 parts by weight of flavor, adjust the pH value of the solution to 7.3-7.5, and prepare a kitchen grease detergent.

9. A kitchen grease detergent prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the kitchen oil stain detergent as claimed in claim 9 in cleaning oil stains and stains.

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