Application of benzyl alcohol in preparation of medicine for promoting healing of scalded wounds

Abstract

The invention discloses an application of benzyl alcohol in preparation of a medicine for promoting healing of scalded wounds, belongs to the field of biological medicines, and provides and verifies the application of benzyl alcohol in treatment of healing of scalded wounds for the first time, and particularly, animal scald model experiments fully prove that benzyl alcohol promotes healing of scalded wounds so as to provide a new thought for scald treatment. The scald wound healing medicine provided by the invention is an old medicine for new use, has the characteristics of safety, effectiveness, low cost and remarkable wound healing promoting effect, and has important clinical significance on scald wound healing.

Description

Technical Field

[0001] The invention relates to the field of biomedicine, and in particular to application of benzyl alcohol in preparing a medicine for promoting healing of scald wounds. Background Art

[0002] Burns are a common accidental skin injury. During the treatment process, it will be accompanied by itching and pain. If the treatment is not good, scars and functional disorders will be formed. At present, burns are generally divided into first-degree burns, second-degree burns and third-degree burns according to the degree of burns. First-degree burns only damage the surface of the skin. The burned area has obvious pain and mild redness and swelling but no blisters. Second-degree burns are divided into shallow second-degree burns and deep second-degree burns. Shallow second-degree burns damage the superficial layers of the epidermis and dermis. The burned skin will turn red and form clear blisters. Deep second-degree burns damage the deep layers of the dermis. The burned skin is red, swollen and white with blood blisters. Third-degree burns mainly damage subcutaneous fat, muscles or bones. The burned skin is gray or reddish brown. Burns are not only physical injuries. Patients who have been burned also bear a great psychological burden. These psychological problems also have a profound impact on the patient's future life and development.

[0003] Benzyl alcohol is often used clinically as a solvent for vitamin B injection and a preservative for ointments or liquid medicines, but there is no literature report that benzyl alcohol can promote the healing of burn wounds. Summary of the invention

[0004] In view of the above problems, the object of the present invention is to provide the use of benzyl alcohol in the preparation of a drug for promoting the healing of scald wounds.

[0005] The benzyl alcohol is benzyl alcohol injection, and the experimental animals involved are ICR mice.

[0006] The medicine contains benzyl alcohol and may further contain pharmaceutically acceptable excipients.

[0007] Furthermore, the degree of burns of the scald wound: the burns in the scald model were deep second degree burns.

[0008] In the study of preparing drugs to promote the healing of burn wounds, the usage and dosage of benzyl alcohol are as follows: wet compresses are applied to the burn wounds of 4-week-old ICR mice, and benzyl alcohol injection is applied once a day, each dose is 100 μL, and continues for 21 days.

[0009] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention proposes and confirms for the first time the use of benzyl alcohol in the preparation of a drug for promoting the healing of scald wounds. The scald wounds of ICR mice were used as the research object. By continuous wet compress for 21 days, benzyl alcohol had the effect of promoting the healing of scald wounds compared with the control group. The drug for promoting the healing of scald wounds provided by the present invention is an old drug with a new use, and has the characteristics of significant effect in promoting wound healing. It is expected to become a new treatment scheme for promoting wound healing, and has a very broad market prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is the result of scald wound modeling in the embodiment of the present invention.

[0011] Figure 2 These are photos of the degree of wound healing at different time points after drug administration at 0.5h, 8h, and 12h after blanching in the examples of the present invention.

[0012] Figure 3 These are H&E staining images of wound tissues at 3, 5, 10, 14, 18 and 21 days after treatment in different groups in the embodiments of the present invention; in the figure, group A was given PBS 0.5 h after scalding, group B was given silver sulfadiazine 0.5 h after scalding, group C was given benzyl alcohol 0.5 h after scalding, group D was given silver sulfadiazine 8 h after scalding, group E was given benzyl alcohol 8 h after scalding, group F was given silver sulfadiazine 12 h after scalding, and group G was given benzyl alcohol 12 h after scalding.

[0013] Figure 4 These are Mason staining images of wound tissues at 3, 5, 10, 14, 18, and 21 days after treatment in different groups in the embodiments of the present invention; in the figure, group A was given PBS 0.5 h after scalding, group B was given silver sulfadiazine 0.5 h after scalding, group C was given benzyl alcohol 0.5 h after scalding, group D was given silver sulfadiazine 8 h after scalding, group E was given benzyl alcohol 8 h after scalding, group F was given silver sulfadiazine 12 h after scalding, and group G was given benzyl alcohol 12 h after scalding. DETAILED DESCRIPTION

[0014] The present invention is further described in detail below with specific embodiments, but it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention.

[0015] Unless otherwise specified, all reagents and materials used in the present invention are commercially available.

[0016] Example: Effect of benzyl alcohol on promoting wound healing after burns 1. Establishment of burn wound model Male ICR mice (4 weeks, body weight = 27 ± 2g) were selected for this experiment and caged for 7 days before the experiment to adapt to the environment. The scald model process in this study was as follows: All mice were anesthetized with 3% sodium pentobarbital (1mL / 100mg) intraperitoneally, the dorsal hair was removed with depilatory cream, and the scratch area was disinfected with 75% ethanol. Then a 100℃ constant temperature heater plate (diameter: 10mm) was used to touch the scratch area of ​​the mouse for 10s. The contact process was not applied by external force, but only depended on the weight of the iron. After 24h of modeling, 3 mice were randomly selected, the scald site and surrounding skin tissue were incised, and fixed with polyformaldehyde solution. H&E and Mason staining were performed to test whether the model was successful.

[0017] 2. Treatment of burn wounds The mice with successful scald wound model were randomly divided into the following groups: Group A: given PBS 0.5 h after scalding, Group B: given silver sulfadiazine 0.5 h after scalding, Group C: given benzyl alcohol 0.5 h after scalding, Group D: given silver sulfadiazine 8 h after scalding, Group E: given benzyl alcohol 8 h after scalding, Group F: given silver sulfadiazine 12 h after scalding, Group G: given benzyl alcohol 12 h after scalding.

[0018] First, the scald wound was sutured around the wound with a silicone ring of appropriate size to prevent the original mouse skin from contracting to promote wound healing. Then, a 10mm*10mm gauze was applied to the wound, and the corners of the gauze were placed at the bottom of the silicone ring. The wet dressing method was completed. Then 100μL of benzyl alcohol injection was dripped on the gauze, and the mouse was allowed to stand for 30s to allow the benzyl alcohol injection to soak the entire scalded surface. This method was repeated once a day for a total of 21 days. Photos were taken to observe the degree of healing of the scald wound.

[0019] The specific dosing methods for different groups are as follows: Group A: 0.5 hours after the burn, take 100 μL of PBS solution and drop it on the gauze sutured with the silicone ring. Let the mouse stand for 30 seconds to allow the PBS solution to soak the entire burn surface. This method was repeated once a day for a total of 21 days. Take photos to observe the degree of healing of the burn wound.

[0020] Group B: 0.5 hours after the burn, silver sulfadiazine was applied to the burn wound sutured by the silicone ring, and the mice were allowed to stand for 30 seconds to allow the silver sulfadiazine to penetrate into the mice. This was done once a day for a total of 21 days. Photos were taken to observe the degree of healing of the burn wound.

[0021] Group C: 0.5 hours after the burn, take 100 μL of benzyl alcohol injection and drop it on the gauze sutured by the silicone ring. Let the mouse stand for 30 seconds to allow the benzyl alcohol injection to soak the entire burn surface. This method was repeated once a day for a total of 21 days. Take photos to observe the degree of healing of the burn wound.

[0022] Group D: 8 hours after the burn, silver sulfadiazine was applied to the burn wound sutured by the silicone ring, and the mice were allowed to stand for 30 seconds to allow the silver sulfadiazine to penetrate into the mice. This was done once a day for a total of 21 days. Photos were taken to observe the degree of healing of the burn wound.

[0023] Group E: 8 hours after the burn, take 100 μL of benzyl alcohol injection and drop it on the gauze sutured by the silicone ring. Let the mouse stand for 30 seconds to allow the benzyl alcohol injection to soak the entire burn surface. This method was repeated once a day for a total of 21 days. Take photos to observe the degree of healing of the burn wound.

[0024] Group F: 12 hours after the burn, silver sulfadiazine was applied to the burn wound sutured by the silicone ring, and the mice were allowed to stand for 30 seconds to allow the silver sulfadiazine to penetrate into the mice. This was done once a day for a total of 21 days. Photos were taken to observe the degree of healing of the burn wound.

[0025] Group G: 12 hours after the burn, take 100 μL of benzyl alcohol injection and drop it on the gauze sutured by the silicone ring. Let the mouse stand for 30 seconds to allow the benzyl alcohol injection to soak the entire burn surface. This method was repeated once a day for a total of 21 days. Take photos to observe the degree of healing of the burn wound.

[0026] 3. Experimental results (1) If Figure 1 As shown in the figure, the burn wound is pale and inelastic. H&E staining shows complete necrosis of the epidermis, fractured and degenerated collagen fibers, and a large number of neutrophils in the superficial dermis. Mason staining shows that a large number of inflammatory cells (such as neutrophils and monocytes) are visible, and the nuclei are dark blue.

[0027] (2) Figure 2 The following are photos of wound healing at different time points after administration of the drug at 0.5h, 8h, and 12h after scalding. The wounds treated with benzyl alcohol healed better than those treated with silver sulfadiazine when the drug was administered at 0.5h, 8h, and 12h after scalding. The wounds treated at 0.5h after scalding healed faster than those treated at 8h and 12h.

[0028] (3) Figure 3 The H&E staining images of wound tissues after treatment in different groups on days 3, 5, 10, 14, 18, and 21. The drugs were administered at 0.5h, 8h, and 12h after the burn. The wound surface treated with benzyl alcohol began to proliferate fibroblasts first, forming granulation tissue, and the keratinocytes of the epidermis began to cover the wound surface, forming a new skin barrier.

[0029] (4) Figure 4Masson-stained images of wound tissues at 3, 5, 10, 14, 18, and 21 days after treatment in different groups. The wounds treated with benzyl alcohol healed better than those treated with silver sulfadiazine at 0.5, 8, and 12 hours after the burn, with a significant increase in the number of collagen fibers, a more compact and regularly arranged blue-green area of ​​the tissue, and granulation tissue gradually replaced by mature collagen fibers.

Claims

1. Benzyl alcohol is used in the preparation of drugs to promote the healing of burn wounds.

2. The use according to claim 1, characterized in that: The benzyl alcohol is benzyl alcohol injection.

3. The use according to claim 1, characterized in that: The medicament comprises benzyl alcohol.

4. The use according to claim 1, characterized in that: The scald wound has a scald degree of deep second degree scald.

5. The use according to claim 1, characterized in that: The experimental animals used were ICR mice.

6. The use according to claim 5, characterized in that: In the study of preparing drugs to promote the healing of burn wounds, the usage and dosage of benzyl alcohol are as follows: wet compresses are applied to the burn wounds of 4-week-old ICR mice, and benzyl alcohol injection is applied once a day, each dose is 100 μL, and continues for 21 days.

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