Regulator for regulating and controlling microbial activity of soil at roots of tea trees as well as preparation method and application of regulator

Abstract

The invention relates to the technical field of tea tree planting, in particular to a regulator for regulating and controlling microbial activity of soil at the root of a tea tree and a preparation method and application of the regulator. The regulator disclosed by the invention is prepared from the following components in parts by mass: 20 to 30 parts of soybean straws, 30 to 40 parts of peanut straws, 20 to 30 parts of duckweed, 8 to 16 parts of mushroom residues, 5 to 10 parts of nostoc commune, 2 to 5 parts of cane sugar, 0.4 to 0.8 part of fungicide and 80 to 100 parts of water. The conditioning agent prepared according to the components of the conditioning agent can increase the number and quantity of nitrogen-fixing microorganisms in tea tree rhizosphere soil, reduce the volume weight of the tea tree rhizosphere soil, improve the porosity and increase the nutrient content of the soil, so that the tea yield and the tea quality are improved, and a basis is provided for harmless planting of tea trees.

Description

Technical Field

[0001] The invention relates to the technical field of tea tree planting, and in particular to a regulator for regulating the activity of soil microorganisms at the roots of tea trees, and a preparation method and application thereof. Background Art

[0002] The composition and quantity of tea rhizosphere microorganisms have an important impact on the physiology and development of tea trees, and are crucial to improving the tea rhizosphere ecosystem. The interaction between tea tree roots and rhizosphere microorganisms is mainly reflected in the following aspects:

[0003] (1) Improve soil structure: The activity of microorganisms can increase the porosity of the soil, improve the aeration and water retention of the soil, and create a good growth environment for the growth of tea tree roots.

[0004] (2) Increase soil fertility: Microorganisms can decompose organic matter in the soil and convert it into nutrients that can be absorbed by tea trees, thereby improving soil fertility.

[0005] (3) Enhance the resistance of tea trees to stress: Microorganisms help improve the resistance of tea trees to diseases and pests, and improve their adaptability to adverse environments.

[0006] (4) Promote the growth of tea trees: Microorganisms can produce plant hormones, stimulate the growth and development of tea tree roots, increase the growth rate of tea trees, and increase tea production.

[0007] (5) Improving tea quality: Microorganisms improve the quality of tea by improving the soil environment, increasing soil nutrients, and promoting the synthesis of theanine in tea.

[0008] It can be seen that tea rhizosphere microorganisms affect all aspects of tea trees. However, unreasonable fertilization by people leads to changes in the composition and quantity of tea rhizosphere microorganisms, which in turn leads to poor root growth of tea trees and poor tea quality. A regulator that regulates the activity of tea tree root microorganisms is urgently needed to meet the deficiencies of the existing technology. Summary of the invention

[0009] The purpose of the present invention is to provide a regulator for regulating nitrogen-fixing microorganisms at the roots of tea trees, promoting the growth of the root system of tea trees, and increasing tea yield, and in particular to a regulator for regulating the activity of soil microorganisms at the roots of tea trees, and a preparation method and application thereof.

[0010] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0011] The present invention provides a regulator for regulating the activity of soil microorganisms at the roots of tea trees, which is prepared from the following components in parts by mass:

[0012] 20-30 parts of soybean straw, 30-40 parts of peanut straw, 20-30 parts of duckweed, 8-16 parts of mushroom residues, 5-10 parts of common Nostoc, 2-5 parts of sucrose, 0.4-0.8 parts of bacterial agent, and 80-100 parts of water.

[0013] Preferably, the particle size of the soybean straw is ≤2 mm;

[0014] The particle size of the peanut straw is ≤2 mm.

[0015] Preferably, the bacterial agent includes nitrogen-fixing bacteria, actinomycetes and Paecilomyces lilacinus;

[0016] The mass ratio of the nitrogen-fixing bacteria: actinomycetes: Paecilomyces lilacinus is 2-3:1-2:1-2;

[0017] The initial live bacterial concentration of the nitrogen-fixing bacteria is ≥3.0×10 9 cfu / g;

[0018] The initial live bacterial concentration of the actinomycetes is ≥1.0×10 10 cfu / g;

[0019] The initial viable bacterial concentration of the described Paecilomyces lilacinus is ≥1.0×10 9 cfu / g.

[0020] The present invention also provides a method for preparing the regulator, comprising the following steps:

[0021] (1) Mixing soybean straw, peanut straw, duckweed, mushroom residue, bacterial agent and 1 / 2 to 2 / 3 of water to obtain a mixture 1; fermenting the mixture 1 for 8 to 12 days, filtering, and taking the filtrate to obtain a first fermentation liquid;

[0022] (2) adding the remaining water to the filter residue remaining after filtering in step (1) to obtain a mixture 2; mixing the mixture 2 with common Nostoc, fermenting for 6 to 10 days, filtering, and collecting the filtrate to obtain a second fermentation liquid;

[0023] (3) After combining the first fermentation liquid and the second fermentation liquid, a mixed liquid is obtained; the mixed liquid is mixed with sucrose, filtered, and the filtrate is collected to obtain a regulator.

[0024] Preferably, the fermentation temperature in step (1) is 25-30°C;

[0025] The filtration in step (1) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

[0026] Preferably, the fermentation temperature in step (2) is 30-35°C;

[0027] The filtration in step (2) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

[0028] Preferably, the filtration in step (3) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

[0029] The present invention also provides the use of the regulator or the regulator prepared by the preparation method in regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of the root system of tea trees, and increasing the yield of tea leaves.

[0030] The present invention also provides the use of the regulator or the regulator prepared by the preparation method in preparing a product for regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of the root system of tea trees, and increasing the yield of tea leaves.

[0031] The present invention also provides a method for regulating soil microorganisms at the roots of tea trees, comprising the following steps:

[0032] Using the regulator or the regulator prepared by the preparation method to perform root irrigation treatment on tea trees;

[0033] During the root irrigation treatment, the regulator is diluted 80 to 100 times with water;

[0034] The application rate of diluted regulator for each root irrigation treatment is 30-70 mL / plant;

[0035] The time for root irrigation is March to April, May to June and September to October every year.

[0036] The present invention provides a regulator for regulating the activity of soil microorganisms at the root of tea trees, and a preparation method and application thereof. The regulator for regulating the activity of soil microorganisms at the root of tea trees is prepared from soybean straw, peanut straw, duckweed, mushroom residue, common Nostoc, sucrose, bacterial agent and water. Duckweed contains nitrogen-fixing bacteria that can fix nitrogen in the air. After fermentation, duckweed combines nitrogen-fixing bacteria contained in itself and nitrogen-fixing bacteria present in common Nostoc with nitrogen in the air and soil to fix nitrogen, which can change the microbial activity between the rhizosphere of tea tree soil, increase the number of nitrogen-fixing microorganisms, and increase the nitrogen content in the rhizosphere, thereby achieving the effect of strengthening and promoting roots. Soybeans and peanuts are leguminous crops, which contain a large number of rhizobia, that is, soybean straw and peanut straw contain a large number of rhizobia. These rhizobia promote the elongation of the root system and the growth of lateral roots by releasing a plant growth hormone called caulobacterin, thereby improving the tea tree's ability to absorb nutrients in the soil. Mushroom residue, peanut straw and soybean straw are also rich in nutrients such as cellulose, protein and sugar, which provide nutrients for the growth of beneficial bacteria and tea trees. Sucrose, as a carbon source, can not only provide carbon source components for the reproduction of beneficial bacteria, but also improve the quality of tea.

[0037] In summary, the components in the regulator of the present invention work together to increase the number and activity of nitrogen-fixing microorganisms in the rhizosphere of tea trees, promote the elongation of the root system and the formation of lateral roots of tea trees, and improve the yield and quality of tea leaves. DETAILED DESCRIPTION

[0038] The present invention provides a regulator for regulating the activity of soil microorganisms at the roots of tea trees, which is prepared from the following components in parts by mass:

[0039] 20-30 parts of soybean straw, preferably 25 parts;

[0040] 30-40 parts of peanut straw, preferably 35 parts;

[0041] 20-30 parts of duckweed, preferably 25 parts;

[0042] 8 to 16 parts of mushroom residue, preferably 12 parts;

[0043] 5-10 parts of common Nostoc, preferably 8 parts;

[0044] 2 to 5 parts of sucrose, preferably 3 parts;

[0045] 0.4-0.8 parts of bacterial agent, preferably 0.6 parts;

[0046] 80 to 100 parts of water, preferably 90 parts.

[0047] In the present invention, the particle size of the soybean straw is ≤2 mm, preferably 1 mm;

[0048] The particle size of the peanut straw is ≤2 mm, preferably 1 mm.

[0049] In the present invention, the bacterial agent includes nitrogen-fixing bacteria, actinomycetes and Paecilomyces lilacinus;

[0050] The mass ratio of the nitrogen-fixing bacteria: actinomycetes: Paecilomyces lilacinus is 2-3:1-2:1-2, preferably 2.5:1.5:1.5; the nitrogen-fixing bacteria is purchased from Shanghai Collection Biotechnology Center (SHBCCD11138); the initial viable bacterial concentration of the nitrogen-fixing bacteria is ≥3.0×10 9 cfu / g, preferably 3.0×10 9 cfu / g; the actinomycetes were purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd., and the initial viable bacterial concentration of the actinomycetes was ≥1.0×10 10 cfu / g, preferably 1.0×10 10 cfu / g; the Paecilomyces lilacinus was purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd., and the initial viable bacterial concentration of the Paecilomyces lilacinus was ≥1.0×10 9cfu / g, preferably 1.0×10 9 cfu / g.

[0051] The present invention also provides a method for preparing the regulator, comprising the following steps:

[0052] (1) Mixing soybean straw, peanut straw, duckweed, mushroom residue, bacterial agent and 1 / 2 to 2 / 3 of water to obtain a mixture 1; fermenting the mixture 1 for 8 to 12 days, filtering, and taking the filtrate to obtain a first fermentation liquid;

[0053] (2) adding the remaining water to the filter residue remaining after filtering in step (1) to obtain a mixture 2; mixing the mixture 2 with common Nostoc, fermenting for 6 to 10 days, filtering, and collecting the filtrate to obtain a second fermentation liquid;

[0054] (3) After combining the first fermentation liquid and the second fermentation liquid, a mixed liquid is obtained; the mixed liquid is mixed with sucrose, filtered, and the filtrate is collected to obtain a regulator.

[0055] In the present invention, the amount of water added is preferably 7 / 12 of the total mass of water; in the present invention, the fermentation temperature in step (1) is 25-30°C, preferably 27.5°C; in the present invention, the fermentation time is preferably 10 days. The filtration in step (1) is membrane filtration, and the pore size of the membrane is 0.2-0.5 μm, preferably 0.45 μm.

[0056] In the present invention, the fermentation temperature in step (2) is 30-35°C, preferably 32°C; the fermentation time in step (2) is preferably 8 days; the filtration in step (2) is membrane filtration, and the pore size of the membrane is 0.2-0.5 μm, preferably 0.45 μm.

[0057] In the present invention, the filtration in step (3) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm, preferably 0.22 μm.

[0058] The present invention also provides the use of the regulator or the regulator prepared by the preparation method in regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of the root system of tea trees, and increasing the yield of tea leaves.

[0059] The present invention also provides the use of the regulator or the regulator prepared by the preparation method in preparing a product for regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of the root system of tea trees, and increasing the yield of tea leaves.

[0060] The present invention also provides a method for regulating soil microorganisms at the roots of tea trees, comprising the following steps:

[0061] Using the regulator or the regulator prepared by the preparation method to perform root irrigation treatment on tea trees;

[0062] During the root irrigation treatment, the pH of the regulator is adjusted to 5.0 to 5.5, preferably 5.3, and then diluted 80 to 100 times with water, preferably 90 times;

[0063] The application amount of the diluted regulator for each root irrigation treatment is 30-70 mL / plant; for tea trees with a tree age of less than 3 years, the application amount for each root irrigation treatment is preferably 30-40 mL / plant, preferably 35 mL / plant; for tea trees with a tree age of 3-5 years, the application amount for each root irrigation treatment is preferably 41-50 mL / plant, preferably 45 mL / plant; for tea trees with a tree age of 5-8 years, the application amount for each root irrigation treatment is preferably 51-60 mL / plant, preferably 55 mL / plant; for tea trees with a tree age of more than 8 years, the application amount for each root irrigation treatment is preferably 70 mL / plant.

[0064] The root irrigation time is from March to April, May to June and September to October every year. In March to April, the root irrigation is carried out 2 to 3 times, once every 10 to 15 days. In May to June, the root irrigation is carried out 3 to 4 times, once every 5 to 10 days. In September to October, the root irrigation is carried out 2 to 3 times, once every 10 to 15 days.

[0065] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0066] The nitrogen-fixing bacteria described in the examples of the present invention were purchased from Shanghai Collection Biotechnology Center (SHBCCD11138), and the initial viable bacteria concentration was 3.0×10 9 cfu / g; the actinomycetes were purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd., and the initial viable bacteria concentration was 1.0×10 10 cfu / g; the Paecilomyces lilacinus was purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd., and the initial viable bacteria concentration was 1.0×10 9 cfu / g.

[0067] The mushroom residue described in the embodiment of the present invention is mushroom residue purchased from Huamo (Shandong) Biotechnology Co., Ltd.

[0068] Example 1

[0069] Take 0.3 kg of nitrogen-fixing bacteria, 0.1 kg of actinomycetes, and 0.15 kg of Paecilomyces lilacinus and mix them to obtain a bacterial agent for later use.

[0070] Take soybean straw and crush it into particles with a diameter of 1 mm for later use;

[0071] Take peanut straw and crush it into particles of 1 mm and set aside.

[0072] Mix 25kg of soybean straw, 30kg of peanut straw, 30kg of duckweed, 10kg of mushroom residue, bacterial agent and 60kg of water to obtain mixture 1. Ferment mixture 1 at 28°C for 10 days, filter with a 0.45μm filter membrane, and take the filtrate to obtain the first fermentation liquid. Add 30kg of water to the residue after taking the filtrate to obtain mixture 2, add 8kg of common Nostoc to mixture 2, mix, ferment at 32°C for 8 days, filter with a 0.45μm filter membrane, and take the filtrate to obtain the second fermentation liquid. After combining the first fermentation liquid and the second fermentation liquid, add 4kg of sucrose, stir evenly, filter with a 0.22μm filter membrane, and take the filtrate to obtain a regulator.

[0073] Example 2

[0074] Take 0.25 kg of nitrogen-fixing bacteria, 0.2 kg of actinomycetes, and 0.2 kg of Paecilomyces lilacinus and mix them to obtain a bacterial agent for later use.

[0075] Take soybean straw and crush it into particles with a diameter of 1 mm for later use;

[0076] Take peanut straw and crush it into particles of 1 mm and set aside.

[0077] Mix 30kg of soybean straw, 35kg of peanut straw, 20kg of duckweed, 8kg of mushroom residue, bacterial agent and 50kg of water to obtain mixture 1. Ferment mixture 1 at 30℃ for 8d, filter with 0.45μm filter membrane, and take the filtrate to obtain the first fermentation liquid. Add 50kg of water to the residue after taking the filtrate to obtain mixture 2, add 10kg of common Nostoc to mixture 2, mix, ferment at 35℃ for 6d, filter with 0.45μm filter membrane, and take the filtrate to obtain the second fermentation liquid. After combining the first fermentation liquid and the second fermentation liquid, add 5kg of sucrose, stir evenly, filter with 0.22μm filter membrane, and take the filtrate to obtain the regulator.

[0078] Example 3

[0079] Take 0.2 kg of nitrogen-fixing bacteria, 0.2 kg of actinomycetes, and 0.1 kg of Paecilomyces lilacinus and mix them to obtain a bacterial agent for later use.

[0080] Take soybean straw and crush it into particles with a diameter of 1 mm for later use;

[0081] Take peanut straw and crush it into particles of 1 mm and set aside.

[0082] Mix 20kg of soybean straw, 40kg of peanut straw, 25kg of duckweed, 16kg of mushroom residue, bacterial agent and 40kg of water to obtain mixture 1. Ferment mixture 1 at 25°C for 12 days, filter with a 0.45μm filter membrane, and take the filtrate to obtain the first fermentation liquid. Add 40kg of water to the residue after taking the filtrate to obtain mixture 2, add 5kg of common Nostoc to mixture 2, mix, ferment at 30°C for 10 days, filter with a 0.45μm filter membrane, and take the filtrate to obtain the second fermentation liquid. After combining the first fermentation liquid and the second fermentation liquid, add 2kg of sucrose, stir evenly, filter with a 0.22μm filter membrane, and take the filtrate to obtain a regulator.

[0083] Comparative Example 1

[0084] The regulator of Comparative Example 1 was set according to the scheme of Example 1, and the first fermentation liquid obtained in Example 1 was used as the regulator of Comparative Example 1.

[0085] Comparative Example 2

[0086] Weigh each component according to the mass fraction of each component in Example 1 for later use, mix soybean straw, peanut straw, duckweed, mushroom residue, bacterial agent, common Nostoc, sucrose and water, ferment at 30°C for 12 days, filter through a 0.22 μm filter membrane, and take the filtrate to obtain a regulator.

[0087] Comparative Example 3

[0088] The regulator of Comparative Example 3 was prepared according to the method of Example 1, except that the mixed liquid obtained by combining the first fermentation liquid and the second fermentation liquid was used as the regulator.

[0089] Comparative Example 4

[0090] The regulator of Comparative Example 4 was prepared according to the method of Example 1, except that duckweed was not added during the first fermentation liquid preparation process.

[0091] Comparative Example 5

[0092] The regulator of comparative example 5 was prepared according to the method of example 1, except that common Nostoc was not added during the second fermentation, and the filtrate obtained by fermenting the remaining residue again was directly used as the second fermentation liquid. After combining the two fermentation liquids, sucrose was added, filtered, and the filtrate was taken to obtain the regulator.

[0093] Experimental Example 1

[0094] The regulators of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5 were adjusted to pH 5.5 and then diluted 100 times with water for standby use.

[0095] Healthy semi-lignified Zhongcha 108 branches were selected as cuttings, each cutting retained 4 complete leaves, and each leaf had a complete axillary bud at the base. The cuttings were randomly divided into 7 groups, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group and control group, with 15 cuttings in each group.

[0096] The cuttings of Example 1 and Comparative Examples 1 to 5 were soaked in the corresponding regulator diluted 100 times for 30 minutes, and then cut into the seedling medium containing perlite. The cuttings of the control group were soaked in a 500 mg / L indoleacetic acid aqueous solution for 30 minutes, and then cut into the seedling medium containing perlite. The cuttings of Example 1 and Comparative Examples 1 to 5 were irrigated with 30 mL / plant of the regulator diluted 100 times of the corresponding group every 10 days, and the control group was not treated in any way. After 60 days of cultivation, the survival of each group of tea trees was observed and the survival rate was calculated; each group of cuttings was pulled out, the number of rooted cuttings was counted, and the average number of roots was calculated; the root length of the longest root of the cuttings was measured. The results are shown in Table 1.

[0097] Survival rate = (number of surviving plants / number of cuttings) × 100%;

[0098] Average number of roots = total number of roots / number of surviving cuttings.

[0099] Table 1 Effects of different regulators on rooting of cuttings

[0100] Group Survival rate% Average number of hair roots / root Longest root length / cm Example 1 100 3.7 7.8 Comparative Example 1 86.7 1.38 2.4 Comparative Example 2 100 2.27 4.3 Comparative Example 3 100 2.40 5.1 Comparative Example 4 93.3 1.86 3.7 Comparative Example 5 93.3 1.71 3.1 Control group 100 2.33 3.7

[0101] Table 1 shows that after using the regulator of Example 1, the survival rate reached 100%, the average number of roots reached 3.7, and the longest root was 7.8 cm long. The root growth was stronger and the number of roots was greater than that of the groups using other groups of regulators and hormones. This shows that the regulator of the present invention can promote the rooting of tea trees and the elongation of the root system of tea trees.

[0102] Experimental Example 2

[0103] The experiment was conducted in the 12-year-old Zhongcha 108 tea garden.

[0104] The regulators of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5 were adjusted to pH 5.5 and then diluted 100 times with water for standby use.

[0105] The test tea trees were randomly divided into 7 groups, Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5 and Control Group, each group of 2 mu, each group of 3 repetitions. Before the test, the soil of 0-40 cm in the rhizosphere of each group of tea trees was randomly taken to detect the number of species and individual number of nitrogen-fixing microorganisms in the rhizosphere soil of different groups of tea trees, the bulk density and total porosity of the rhizosphere soil, and the total nitrogen, total phosphorus and effective potassium content in the rhizosphere soil. The results are shown in Tables 2-3. On March 5, the tea trees of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5 were irrigated with 70 mL / plant of the root of the tea tree. The root irrigating agent was a 100-fold diluted regulator for the corresponding group, and the root was irrigated once every 15 days, and the root was irrigated twice in total. The control group was irrigated with the same dose of water. On the 10th day after the second root irrigation, the soil from 0 to 40 cm in the rhizosphere of each group of tea trees was collected to detect the number of species and individuals of nitrogen-fixing microorganisms in the rhizosphere soil of different groups of tea trees, the bulk density and total porosity of the rhizosphere soil, and the total nitrogen, total phosphorus and available potassium content in the rhizosphere soil. The results are shown in Tables 2 and 3. Nitrogen-fixing microorganisms include active nitrogen-fixing microorganisms, micro-nitrogen-loving microorganisms, and combined nitrogen-fixing microorganisms.

[0106] Table 2 Effects of different regulators on microorganisms and soil properties in the rhizosphere soil of tea trees

[0107]

[0108]

[0109] Table 2 shows that the conditioning agent of Example 1 can increase the quantity and number of nitrogen-fixing microorganisms in the soil, reduce the soil bulk density, and increase the soil porosity.

[0110] Table 3 Effects of different regulators on nutrients in the rhizosphere soil of tea trees

[0111]

[0112] Table 3 shows that the treatment of tea rhizosphere soil with the regulator of Example 1 increases the content of total nitrogen, total phosphorus and available potassium in the soil. The increase in total nitrogen, total phosphorus and available potassium content in the control group after treatment is because tea tree organic fertilizer produced by Hebei Wanbang Fertilizer Leaf Co., Ltd. was applied to the tea trees before the first application of the regulator, so the total nitrogen, total phosphorus and available potassium content in the control group after treatment is higher than that before treatment.

[0113] Experimental Example 3

[0114] The regulators of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5 were adjusted to pH 5.5 and then diluted 100 times with water for standby use.

[0115] Continue to plant tea trees of each group according to the method of Experimental Example 2. On May 5, the tea trees of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5 were root-irrigated at an amount of 70 mL / plant, and the root-irrigating agent was a 100-fold dilution of the corresponding group, and the root-irrigation was performed once every 8 days, and the root-irrigation was performed 4 times in total, and the control group was root-irrigated with a corresponding amount of water. On September 6, the tea trees of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5 were root-irrigated at an amount of 70 mL / plant, and the corresponding group was root-irrigated with a 100-fold dilution of the regulator, and the root-irrigation was performed once every 15 days, and the root-irrigation was performed 3 times in total, and the control group was root-irrigated with a corresponding amount of water. Tea leaves were harvested at different times, and the yield of one bud and two leaves in spring tea, summer tea, and autumn tea was counted, and the results are shown in Table 4. The content of amino acids in autumn tea treated with different adjustments was counted. The results are shown in Table 4.

[0116] Table 4 Effects of different regulators on tea yield and quality

[0117]

[0118] Table 4 shows that the tea trees treated with the regulator of Example 1 can significantly increase the yield of spring tea, summer tea and autumn tea compared with the control group, and can also increase the content of amino acids in tea leaves. It can be seen that the regulator of the present invention has the effect of increasing the yield and quality of tea leaves.

[0119] As can be seen from the above embodiments, the present invention provides a regulator for regulating the activity of soil microorganisms at the root of tea trees, and a preparation method and application thereof. The regulator for regulating the activity of soil microorganisms at the root of tea trees is prepared by soybean straw, peanut straw, duckweed, mushroom residue, common Nostoc, sucrose, bacterial agent and water. Duckweed contains nitrogen-fixing bacteria that can fix nitrogen in the air. After fermentation, duckweed combines nitrogen-fixing bacteria contained in itself and nitrogen-fixing bacteria present in common Nostoc species with nitrogen in the air and soil to fix nitrogen, which can change the microbial activity between the rhizosphere of tea tree soil, increase the number of nitrogen-fixing microorganisms, and increase the nitrogen content in the rhizosphere, thereby achieving the effect of strengthening roots and promoting roots. Soybeans and peanuts are leguminous crops, and leguminous crops contain a large number of rhizobia, that is, soybean straw and peanut straw contain a large number of rhizobia. These rhizobia promote the elongation of the root system and the growth of lateral roots by releasing a plant growth hormone called caulobacterin, thereby improving the tea tree's ability to absorb nutrients in the soil. Mushroom residue, peanut straw and soybean straw are also rich in nutrients such as cellulose, protein and sugar, which provide nutrients for the growth of beneficial bacteria and tea trees. Sucrose, as a carbon source, can not only provide carbon source components for the reproduction of beneficial bacteria, but also improve the quality of tea.

[0120] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A regulator for regulating the activity of soil microorganisms at the roots of tea trees, characterized in that: It is prepared from the following components in parts by weight: 20-30 parts of soybean straw, 30-40 parts of peanut straw, 20-30 parts of duckweed, 8-16 parts of mushroom residues, 5-10 parts of common Nostoc, 2-5 parts of sucrose, 0.4-0.8 parts of bacterial agent, and 80-100 parts of water.

2. The regulator according to claim 1, characterized in that The particle size of the soybean straw is ≤2 mm; The particle size of the peanut straw is ≤2 mm.

3. The regulator according to claim 2, characterized in that The bacterial agent includes nitrogen-fixing bacteria, actinomycetes and Paecilomyces lilacinus; The mass ratio of the nitrogen-fixing bacteria: actinomycetes: Paecilomyces lilacinus is 2-3:1-2:1-2; The initial live bacterial concentration of the nitrogen-fixing bacteria is ≥3.0×10 9 cfu / g; The initial live bacterial concentration of the actinomycetes is ≥1.0×10 10 cfu / g; The initial viable bacterial concentration of the described Paecilomyces lilacinus is ≥1.0×10 9 cfu / g.

4. The method for preparing the regulator according to any one of claims 1 to 3, characterized in that: The steps include: (1) Mixing soybean straw, peanut straw, duckweed, mushroom residue, bacterial agent and 1 / 2 to 2 / 3 of water to obtain a mixture 1; fermenting the mixture 1 for 8 to 12 days, filtering, and taking the filtrate to obtain a first fermentation liquid; (2) adding the remaining water to the filter residue remaining after filtering in step (1) to obtain a mixture 2; mixing the mixture 2 with common Nostoc, fermenting for 6 to 10 days, filtering, and collecting the filtrate to obtain a second fermentation liquid; (3) After combining the first fermentation liquid and the second fermentation liquid, a mixed liquid is obtained; the mixed liquid is mixed with sucrose, filtered, and the filtrate is collected to obtain a regulator.

5. The preparation method according to claim 4, characterized in that: The fermentation temperature in step (1) is 25-30°C; The filtration in step (1) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

6. The preparation method according to claim 5, characterized in that: The fermentation temperature in step (2) is 30-35°C; The filtration in step (2) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

7. The preparation method according to claim 6, characterized in that: The filtration in step (3) is membrane filtration, and the pore size of the membrane is 0.2 to 0.5 μm.

8. Use of the regulator according to any one of claims 1 to 3 or the regulator prepared by the preparation method according to any one of claims 4 to 7 in regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of tea tree roots, and increasing tea yield.

9. Use of the regulator according to any one of claims 1 to 3 or the regulator prepared by the preparation method according to any one of claims 4 to 7 in the preparation of products for regulating nitrogen-fixing microorganisms in the roots of tea trees, promoting the growth of tea tree roots, and increasing tea yield.

10. A method for regulating soil microorganisms at the roots of tea trees, characterized in that: The steps include: Using the regulator according to any one of claims 1 to 3 or the regulator prepared by the preparation method according to any one of claims 4 to 7 to perform root irrigation treatment on tea trees; During the root irrigation treatment, the regulator is diluted 80 to 100 times with water; The application rate of diluted regulator for each root irrigation treatment is 30-70 mL / plant; The time for root irrigation is March to April, May to June and September to October every year.

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