Airborne pollen aeration sheet sampling membrane and sampling method

Abstract

The invention provides an air-borne pollen aeration sheet sampling membrane and a sampling method. The air-borne pollen aeration sheet sampling membrane comprises: a substrate; the adhesive layer is arranged on the upper surface of the substrate; the adhesive layer is covered with the release layer, and the substrate, the adhesive layer and the release layer are sequentially arranged in the height extension direction of the airborne pollen aeration sheet sampling membrane; an acquisition area is arranged on the release layer. The pollen collecting device can continuously and efficiently collect pollen while reducing manual intervention. It can be ensured that a sample is kept pure in the collection process, and external pollution is effectively prevented.

Description

Technical Field

[0001] The invention relates to the technical field of environmental monitoring and botanical research, and in particular to an airborne pollen exposure sheet sampling membrane and a sampling method. Background Art

[0002] In the traditional pollen collection method, the use of glass slides for pollen collection involves a complex and labor-intensive manual smearing process. According to the "QXT 42-2006 Airborne Pollen Exposure Method Observation Specification", the steps of pollen sampling mainly include: preparing the pollen sampler, glass slide, cover glass and microscope required for sampling, and preparing adhesives and dyes; installing the pollen sampler in a sheltered location without high-rise buildings, usually in an area with rich vegetation; evenly applying adhesive on the glass slide and placing it in the sampler, exposing it for 24 hours to collect pollen; immediately placing the slide in a box covered with a cover glass after collecting it; staining the glass slide; using a microscope to observe and record the morphology and quantity of pollen; recording the observation data and the weather conditions of the day in detail; regularly reviewing the sampling results, calculating the error by repeating the sample test, and ensuring the accuracy of the data; finally, properly maintaining the equipment used to ensure the continuity of the observation work and the stability of the equipment.

[0003] Factors such as airflow, temperature and humidity in the environment will also affect the stability of the smearing process, increase the complexity and uncertainty of the operation, and there is a risk of sample contamination. In addition, the operating environment must be kept highly clean and the operator's hands need to be thoroughly cleaned, but it is still difficult to completely avoid micro-contamination. Constant environmental conditions need to be controlled during the sample drying process, the drying time is long and susceptible to external interference. The handling of slides and preparation for microscopic observation also increase the complexity of the operation. Therefore, these complex steps in the traditional method are not only time-consuming and prone to errors, but also affect the collection efficiency and sample quality. It is particularly necessary to develop a new type of pollen exposure sampling membrane to simplify the operating process, improve accuracy and efficiency, and reduce dependence on manual operations. Summary of the invention

[0004] In view of this, the embodiments of this specification provide an airborne pollen exposure sampling membrane and a sampling method to achieve the purpose of improving accuracy.

[0005] The embodiments of this specification provide the following technical solutions: an air-transferred pollen exposure sheet sampling membrane, comprising: a substrate; an adhesive layer, arranged on the upper surface of the substrate; a release layer, covering the adhesive layer, and the substrate, the adhesive layer and the release layer are arranged in sequence along the height extension direction of the air-transferred pollen exposure sheet sampling membrane; a collection area is arranged on the release layer.

[0006] Furthermore, the present invention also provides a sampling method, which uses the above-mentioned airborne pollen exposure sampling membrane for sampling.

[0007] Further, the sampling method comprises the following steps:

[0008] Step 1: Tear off the surface material in a clean environment;

[0009] Step 2: Place the airborne pollen sampling film in the collection area, so that the air flow containing pollen hits the collection area and makes the pollen adhere to the surface of the collection area and then flow out from the vents;

[0010] Step 3: After the collection is completed, cover the surface material again and place the airborne pollen exposure film sampling film in the sample box or sealed bag.

[0011] Compared with the prior art, the at least one technical solution adopted in the embodiments of this specification can achieve the following beneficial effects:

[0012] The present invention uses a highly light-transmitting polymer film as a substrate, which has excellent cuttability and easy handling. Operators can easily cut to the required length, which is convenient for pollen collection and simplifies the operation steps. The high toughness of the substrate ensures that it will not be easily damaged during operation.

[0013] The vents in the design optimize the airflow path, ensuring that pollen particles can effectively pass through and adhere to the adhesive layer. This design significantly improves the capture rate of pollen and enhances the overall efficiency and accuracy of collection. The adjustability of the vents allows it to adapt to different environmental conditions.

[0014] The design of the collector includes covering the sample with a protective film or tissue before collection, which prevents the collection area from being contaminated by the outside world before use. This protective measure ensures the purity of the sample during the collection process and improves the reliability of the data. The antimicrobial treatment of the surface material further ensures the sterility of the sample.

[0015] The entire collection device is light and compact, designed to be portable, and is very suitable for use in field environments and laboratories. Its compact design makes it easier to carry and store, providing a flexible solution for different usage scenarios. The device comes with a portable case for easy carrying and protection when collecting outside. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 is a cross-sectional view of an embodiment of the present invention;

[0018] Figure 2 is a top view of an embodiment of the present invention;

[0019] Figure 3 It is a schematic diagram of a top view structure of other examples of the embodiments of the present invention.

[0020] Reference numerals in the figure: 1, base material; 2, adhesive layer; 3, release layer; 31, collection area; 32, ventilation hole; 4, surface material. DETAILED DESCRIPTION

[0021] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0022] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0023] like Figures 1 to 3 As shown, an airborne pollen exposure film sampling membrane comprises: a substrate 1, an adhesive layer 2 and a release layer 3. The adhesive layer 2 is arranged on the upper surface of the substrate 1; the release layer 3 covers the adhesive layer 2, and the substrate 1, the adhesive layer 2 and the release layer 3 are arranged in sequence along the height extension direction of the airborne pollen exposure film sampling membrane; and a collection area 31 is arranged on the release layer 3.

[0024] The embodiment of the present invention can continuously and efficiently collect pollen while reducing manual intervention, ensure that the sample remains pure during the collection process, and effectively prevent external contamination.

[0025] It should be noted that a highly light-transmitting polymer film is used as the substrate 1. The substrate 1 is made of advanced materials with excellent optical transparency and high toughness, ensuring the clarity of optical observation and the stability of mechanical operation. Its excellent light transmittance significantly improves the visibility and convenience of operation during pollen collection. This substrate 1 can not only withstand the influence of various environmental conditions, but also has certain anti-static properties to prevent pollen particles from being offset due to static electricity.

[0026] A layer of high-performance special adhesive is precisely coated on the substrate 1 to form an adhesive layer 2. This adhesive layer 2 has super strong adhesion and excellent transparency, and can efficiently capture tiny pollen particles while maintaining the transparency of the substrate to ensure accurate recording and analysis of pollen samples. The formulation of the adhesive has been optimized and a combination of various polymer materials is used. It can work stably under different environmental conditions and provide a reliable capture effect. The adhesive layer 2 also has a certain waterproof performance to avoid the degradation of the adhesive performance in a high humidity environment. The above-mentioned substrate 1 and adhesive layer 2 are both made of existing materials, and their specific components are not described in detail here.

[0027] The release layer 3 is made of release paper or release film with silicone oil coating. The release layer 3 adopts advanced silicone oil coating technology and has excellent anti-adhesion performance, which can effectively prevent the adhesive from accidentally sticking during storage and transportation, and maintain the best use state of the adhesive layer 2. The release layer 3 is designed to be a reusable structure, which can reduce material consumption and use costs through multiple uses.

[0028] The reuse of the release layer 3 also helps to improve the environmental performance of the sampling film. In environmental monitoring and botanical research, reducing the use of disposable plastics is one of the important measures to achieve sustainable development. By adopting a reusable release layer 3, the waste generated after the use of the sampling film can be reduced and the impact on the environment can be reduced. In addition, this design is also convenient for the cleaning and maintenance of the sampling film. The operator can clean and disinfect the release layer 3 after the collection cycle to ensure the cleanliness and reliability of the next use, thereby improving the efficiency and safety of the entire sampling process. This design not only reflects the consideration of environmental protection, but also meets the requirements of resource conservation and recycling in modern scientific research and industrial production.

[0029] The collecting area 31 is a hole-shaped structure penetrating through the release layer 3 , and the collecting area 31 can connect the upper surface of the adhesive layer 2 with the outer space of the release layer 3 .

[0030] The air-transfer pollen sampling film also includes a vent hole 32, which passes through the release layer 3, the adhesive layer 2 and the base material 1 in sequence and is located outside the collection area 31. The vent hole 32 is connected to the collection area 31. The setting of the vent hole 32 optimizes the capture efficiency of pollen particles. The position and size of the vent hole are precisely calculated to ensure that the air flow has an effective effect on the adhesive layer, thereby improving the accuracy and efficiency of collection.

[0031] In the embodiment of the present invention, the gas is introduced from above the release layer 3 and enters the collection area 31, the pollen particles carried in the gas are adsorbed by the adhesive layer 2, the gas enters from the collection area 31 into the vent holes 32 and is discharged from the vent holes 32, thereby improving the accuracy and efficiency of collection.

[0032] In the present invention, fine airflow management is achieved by optimizing the structural design of the collection area 31, the geometric parameters of the vents 32, and the airflow guidance method in detail. In order to ensure that pollen particles effectively enter and adhere to the adhesive layer 2 during the air flow, the position, size and shape of the vents 32 in the collection area 31 must be accurately designed so that the airflow produces an ideal flow path and pressure distribution when passing through the vents 32, thereby achieving the best capture efficiency.

[0033] like Figure 3As shown, in the embodiment of the present invention, the shape of the collection area can be rectangular, circular, elliptical or other shapes. The shape of the vent hole 32 can be a straight hole or an oblique hole in the shape of a rectangle, circle, ellipse or polygon.

[0034] The inclined hole design can optimize the guidance of airflow, making pollen particles pass through the vents more smoothly, reducing the disturbance of airflow on pollen particles, thereby increasing the adhesion rate of pollen particles on the adhesive layer. In addition, the inclined hole design can also reduce the direct impact of air flow on the sampling membrane, reduce the rebound or loss of pollen particles caused by direct impact of airflow, and ensure that pollen particles can be collected and fixed more effectively.

[0035] The inclined hole design also helps to adapt to different environmental conditions. For example, in an environment with high wind speed, the inclined holes can reduce the direct impact of wind speed on the sampling membrane, maintain the stability of the sampling membrane, and avoid the decrease in pollen collection efficiency due to changes in wind speed. At the same time, the size and angle of the inclined holes can be adjusted according to actual needs to adapt to different pollen types and sizes, as well as different environmental conditions such as humidity and temperature, so as to achieve more accurate pollen collection. This flexibility and adaptability is an important supplement to the sampling membrane design in the existing technology, which helps to improve the accuracy and efficiency of pollen collection.

[0036] In addition to conventional symmetrical vents 32, the present invention also proposes the use of asymmetrical vents 32. This asymmetrical design can guide the airflow to form a vortex effect at the outlet of the air inlet. The vortex can increase the residence time of pollen particles in the collection area 31, increase the probability of their contact with the adhesive layer 2, and thus further improve the sampling efficiency. This structural design is particularly suitable for outdoor collection environments with high wind speeds.

[0037] The above shapes and structures do not limit the present application. All pore structures with gas extraction function should be within the protection scope of the present application, and they are not listed one by one here.

[0038] Preferably, there are a plurality of vents 32, which are arranged at intervals along the periphery of the collection area 31. The vents 32 may be arranged evenly or unevenly.

[0039] The number, diameter and distance between the vent holes 32 directly affect the stability and uniformity of the airflow. The hole spacing between the vent holes 32 is designed to be balanced with each other, so that when the airflow passes through the vent holes 32, an overly concentrated airflow beam is avoided, thereby improving the capture efficiency of the adhesive layer 2. In the present invention, the diameter of the vent holes 32 is usually controlled between 0.5 and 2 mm to ensure that the airflow can pass smoothly without reducing the pollen capture efficiency due to an excessively large aperture. An accurate proportional relationship is formed between the aperture and the airflow velocity.

[0040] Preferably, the positions of the vent holes 32 need to be evenly distributed around the collection area 31 to avoid excessive concentration of airflow in a certain area, which may cause local overuse or underuse of the adhesive layer. In order to achieve the best effect, the layout of the vent holes 32 should be evenly distributed so that pollen particles entering the collection area 31 can be evenly captured.

[0041] The design conditions of the aperture size of the vent hole 32 in the present invention are as follows:

[0042] Small aperture (0.5 to 1 mm): Suitable for high wind speed environments and low pollen particle concentrations. Small apertures can effectively reduce the air flow rate, making it easier for pollen particles to adhere to the adhesive layer. In addition, small apertures can also reduce interference with the adhesive layer and extend its service life.

[0043] Large aperture (1.5 to 2 mm): Suitable for low wind speed or still air environment, or when pollen concentration is high. Large aperture can ensure sufficient airflow to improve the efficiency of pollen collection, especially when air flow is not significant, it can increase the total amount of air entering the collection area 31.

[0044] The design principle of the spacing between the vent holes 32 in the present invention is as follows:

[0045] Smaller spacing: When the airflow is small or the sampling sensitivity needs to be improved, the spacing between the vent holes 32 can be shortened, usually between 2 mm and 5 mm, to ensure sufficient airflow through the aperture while reducing the burden on a single hole, making the pollen particles more evenly distributed.

[0046] Larger spacing: Suitable for environments with large airflow. When the spacing is more than 5 mm, it can reduce the impact of airflow on the adhesive layer and avoid adhesive failure due to excessive airflow.

[0047] The relationship between the shape of the vent hole 32 and the airflow direction in the present invention is as follows:

[0048] Symmetrical holes are suitable for straight-line airflow, ensuring smooth airflow and suitable for a relatively stable environment.

[0049] The asymmetric hole design is suitable for use in an environment where the airflow direction changes frequently. Its asymmetric shape can generate airflow disturbances, thereby increasing the contact opportunities between pollen particles and the adhesive layer 2.

[0050] At the same time, in order to ensure that the number of vents 32 and the area of ​​the adhesive layer are reasonably matched, the total area of ​​the vents 32 should account for 20%-40% of the area of ​​the adhesive layer 2. This can ensure sufficient air circulation without affecting the pollen capture ability of the adhesive layer 2.

[0051] Furthermore, in the operation of the automated machine, the flow rate of the vent 32 can be precisely adjusted by controlling the air intake of the control system. The system automatically adjusts the flow rate of the vent 32 according to real-time meteorological data (such as wind speed, humidity, etc.) to ensure air flow stability and pollen particle capture efficiency during the sampling process.

[0052] Through the above structural design, fine airflow management can achieve efficient capture of pollen particles in the air under different environmental conditions by optimizing the shape, size, position and airflow guide system of the vent 32. The flow regulation of the vent 32 further enhances the adaptability of the invention, ensuring that the sampling membrane can still maintain an efficient and stable working state in a complex and changing environment. This series of innovative designs greatly improves the efficiency, accuracy and reliability of pollen collection.

[0053] The airborne pollen exposure film sampling film also includes a surface material 4, which covers the release layer 3. The surface material 4 is made of a highly transparent plastic film or thin paper, and serves as a protective layer to prevent any form of contamination of the collection area 31 before use. The design of the surface material 4 adopts advanced materials and processes, which can effectively prevent the external environment from interfering with the collection area 31. When the user needs to collect, the surface material 4 can be easily torn off to quickly expose the collection area 31, ensuring cleanliness and efficiency during use. The surface material 4 is treated with antibacterial treatment to prevent the pollen sample from being contaminated by microorganisms during the collection process.

[0054] The pollen exposure sheet sampling membrane of the present invention can realize an automated and continuous pollen collection process. Its efficient and convenient design not only improves the simplicity of operation, but also greatly reduces the risk of contamination, ensuring the high quality and reliability of the collected samples.

[0055] The present invention also provides a sampling method, which uses the above-mentioned airborne pollen exposure sheet sampling membrane for sampling, and the sampling method comprises the following steps:

[0056] Step 1: tear open the surface material 4 in a clean environment;

[0057] Step 2: Place the airborne pollen sampling film in the collection area 31, so that the air flow containing pollen hits the collection area 31, and the pollen adheres to the surface of the collection area 31, and then flows out from the vent 32;

[0058] Step 3: After the collection is completed, cover the surface material 4 again and put the airborne pollen exposure film sampling film in a sample box or a sealed bag.

[0059] Before step 1, preparation operations need to be performed, as follows:

[0060] Cut the airborne pollen sampling film to the required length (if it is manually operated) or install the airborne pollen sampling film into the machine (if it is machine operated), ensuring that the collection area 31 and the vent 32 are intact. When setting up the device, special attention should be paid to the alignment of the collection area 31 and the vent 32 to ensure the consistency and stability of the collection effect.

[0061] The clean environment in step 1 can be operated in a laboratory environment using dust-free gloves to further reduce the risk of contamination.

[0062] The collection steps in step 2 are divided into manual operation and mechanical operation:

[0063] Manual operation: Place the prepared collector (with the airborne pollen sampling film) in the target environment, so that pollen particles in the air enter the collection area 31 through the vents and adhere to the adhesive layer 2. The collector should be kept stable during operation to avoid human interference. It is recommended to use a fixed bracket or other stabilizing equipment to ensure that the collector remains in a fixed position throughout the collection process.

[0064] Machine operation: Install the collector (with airborne pollen sampling film) on the machine, and use the machine's wheel system to automatically switch the target environment. By rotating the wheel, the machine will automatically place the collector in the target environment, and the pollen particles will enter the collection area 31 and adhere to the adhesive layer 2. This method does not require manual cutting, and the operation is simple and efficient. The machine equipment should be equipped with a constant temperature control system to ensure the stability of environmental conditions during the collection process.

[0065] In step 3, the airborne pollen film sampling film is placed in a sample box or sealed bag to ensure the integrity and safety of the sample, which is convenient for subsequent analysis and storage. The sample box should be designed to be shockproof to prevent damage to the sample during transportation.

[0066] The pollen exposure sheet sampling membrane of the present invention supports flexible manual and automatic operation modes, ensuring the high efficiency of pollen collection and the high-quality preservation of samples.

[0067] The above is only a specific embodiment of the present invention, and cannot be used to limit the scope of the invention. Therefore, the replacement of equivalent components, or equivalent changes and modifications made according to the scope of protection of the patent of the present invention, should still fall within the scope of this patent. In addition, the technical features of the present invention can be freely combined with each other, with each other, and with each other.

Claims

1. An airborne pollen exposure sampling membrane, characterized in that: include: Substrate (1); An adhesive layer (2) is disposed on the upper surface of the substrate (1); The release layer (3) covers the adhesive layer (2), and the base material (1), the adhesive layer (2) and the release layer (3) are arranged in sequence along the height extension direction of the airborne pollen exposure sheet sampling membrane; and a collection area (31) is arranged on the release layer (3).

2. The airborne pollen exposure sampling membrane according to claim 1, characterized in that: The release layer (3) is made of release paper or release film with a silicone oil coating.

3. The airborne pollen exposure sampling membrane according to claim 1, characterized in that: The collection area (31) is a hole-shaped structure that penetrates the release layer (3), and the collection area (31) can connect the upper surface of the adhesive layer (2) and the outer space of the release layer (3).

4. The airborne pollen sampling membrane according to claim 1, characterized in that: The air-transferred pollen exposure sheet sampling membrane further comprises a vent hole (32), which sequentially penetrates the release layer (3), the adhesive layer (2) and the base material (1) and is located outside the collection area (31), and the vent hole (32) is connected to the bottom of the collection area (31).

5. The airborne pollen exposure sampling membrane according to claim 4, characterized in that: The vent hole (32) is a straight hole or an oblique hole in a rectangular, circular or polygonal shape.

6. The airborne pollen exposure sampling membrane according to claim 5, characterized in that: There are a plurality of vent holes (32) which are arranged at intervals along the outer periphery of the collection area (31).

7. The airborne pollen sampling membrane according to claim 1, characterized in that: The airborne pollen exposure film sampling membrane also includes a surface material (4) covering the release layer (3).

8. The airborne pollen exposure sampling membrane according to claim 7, characterized in that: The surface material (4) is made of transparent plastic film or thin paper.

9. A sampling method, using an airborne pollen sampling membrane for sampling, characterized in that: The airborne pollen exposure sheet sampling membrane is the airborne pollen exposure sheet sampling membrane described in any one of claims 1 to 8.

10. The sampling method according to claim 9, characterized in that: The sampling method comprises the following steps: Step 1: tear off the surface material (4) in a clean environment; Step 2: placing the airborne pollen exposure sheet sampling membrane in the collection area (31), allowing the air flow containing pollen to impact the collection area (31), and allowing the pollen to adhere to the surface of the collection area (31), and then flow out from the vents (32); Step 3: After the collection is completed, cover the surface material (4) again and place the airborne pollen exposure film sampling film in a sample box or a sealed bag.

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